AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880046549.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046549.01.T01 MTR_4g109830 70.526 95 10 2 7 87 110 200 1.62e-31 110
MsG0880046549.01.T01 MTR_2g461760 46.465 99 35 2 8 88 49 147 3.86e-17 72.0
MsG0880046549.01.T01 MTR_2g461760 56.338 71 17 1 8 64 49 119 7.49e-17 71.2
MsG0880046549.01.T01 MTR_5g046790 44.156 77 29 1 9 71 112 188 1.50e-13 63.5
MsG0880046549.01.T01 MTR_7g016630 44.048 84 32 2 6 75 107 189 4.39e-12 60.1
MsG0880046549.01.T01 MTR_8g066260 50.000 60 16 1 6 51 109 168 9.44e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046549.01.T01 AT5G60910 57.353 68 15 1 7 60 48 115 6.06e-17 71.6
MsG0880046549.01.T01 AT5G60910 57.353 68 15 1 7 60 110 177 1.67e-16 71.6
MsG0880046549.01.T01 AT1G69120 45.455 66 22 1 9 60 50 115 1.48e-11 57.8
MsG0880046549.01.T01 AT1G69120 44.118 68 24 1 7 60 110 177 2.23e-11 57.8
MsG0880046549.01.T01 AT1G69120 44.118 68 24 1 7 60 110 177 3.44e-11 57.8

Find 64 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGGCAACTGCTTTGTTTC+AGG 0.137538 8:+74752570 None:intergenic
TTCTGCTACTATTCATTTAT+AGG 0.193739 8:-74755363 None:intergenic
GTTACAAGCCACCAAGATTT+TGG 0.200402 8:-74755091 None:intergenic
CCTACATTTGCACTATCATT+AGG 0.222452 8:+74754838 MsG0880046549.01.T01:three_prime_UTR
CGAGCAAGTTGTTATGTTCT+TGG 0.265775 8:-74754426 None:intergenic
AGAAAACATCATTACCTTCT+TGG 0.311252 8:-74754449 None:intergenic
CACTAACCCTGTAGTATTCA+TGG 0.329083 8:-74754644 None:intergenic
TCTCAAAGAGCTTCAAAGTT+TGG 0.334932 8:+74752624 MsG0880046549.01.T01:CDS
CTACATTTGCACTATCATTA+GGG 0.339253 8:+74754839 MsG0880046549.01.T01:three_prime_UTR
GAGAGCATTAAATCTCTATA+GGG 0.340061 8:-74754984 None:intergenic
TGAGAGCATTAAATCTCTAT+AGG 0.349672 8:-74754985 None:intergenic
TGACTGAATTTCATTGTTGC+TGG 0.353886 8:+74755547 MsG0880046549.01.T01:three_prime_UTR
GGGGAAGACTTGGATGGTTT+AGG 0.365453 8:+74752601 MsG0880046549.01.T01:CDS
TATATGTTGCATATTCAAAT+AGG 0.369347 8:-74755219 None:intergenic
TAAAATATGCATAGTTATTG+AGG 0.376925 8:-74755117 None:intergenic
TTACTGAGTTGATGCAGATC+TGG 0.379364 8:+74755263 MsG0880046549.01.T01:three_prime_UTR
TCCAATGGTTGAGTTTCAAT+TGG 0.387618 8:-74754619 None:intergenic
TCTTCATTGCAATTCATGTT+GGG 0.391131 8:-74755288 None:intergenic
CGATGATATACTATATCTAT+AGG 0.401689 8:-74755458 None:intergenic
ATAGTATATCATCGATATAT+AGG 0.402522 8:+74755467 MsG0880046549.01.T01:three_prime_UTR
TTCTTCATTGCAATTCATGT+TGG 0.402872 8:-74755289 None:intergenic
GAGAGAGAATAAAATATTGT+AGG 0.408766 8:-74754882 None:intergenic
TGCTGGCCGGCCATACTATA+TGG 0.414688 8:+74755564 MsG0880046549.01.T01:three_prime_UTR
AAAACACAAATACAGCTTGT+AGG 0.421950 8:+74755432 MsG0880046549.01.T01:three_prime_UTR
TATAGCGAGACCATATAGTA+TGG 0.428706 8:-74755574 None:intergenic
TACTGAGTTGATGCAGATCT+GGG 0.436324 8:+74755264 MsG0880046549.01.T01:three_prime_UTR
TTGGATACTCTTATATTCAC+TGG 0.446454 8:+74754788 MsG0880046549.01.T01:three_prime_UTR
TTCAAAGGTTCCCACCTCTC+TGG 0.448054 8:+74754691 MsG0880046549.01.T01:three_prime_UTR
CATTCCAAATGCCAAAATCT+TGG 0.450511 8:+74755080 MsG0880046549.01.T01:three_prime_UTR
AGCAAAGCGTTTATCTCTAT+AGG 0.477083 8:-74754906 None:intergenic
TTGGAATCCATGAATACTAC+AGG 0.477322 8:+74754637 MsG0880046549.01.T01:CDS
TCCAATTGAAACTCAACCAT+TGG 0.486216 8:+74754618 MsG0880046549.01.T01:CDS
GCAAGTTGTTATGTTCTTGG+AGG 0.493736 8:-74754423 None:intergenic
CAAGTTGTTATGTTCTTGGA+GGG 0.519113 8:-74754422 None:intergenic
TGATGTATAATTGAATTCAA+AGG 0.523402 8:+74754676 MsG0880046549.01.T01:exon
TAATTACACAATTACAATGC+AGG 0.526513 8:-74755513 None:intergenic
TCCAAATGCCAAAATCTTGG+TGG 0.528543 8:+74755083 MsG0880046549.01.T01:three_prime_UTR
ATCGATATATAGGCATATGA+TGG 0.529791 8:+74755477 MsG0880046549.01.T01:three_prime_UTR
CCTAATGATAGTGCAAATGT+AGG 0.530968 8:-74754838 None:intergenic
TTATAAATAGAGCTAATTAG+AGG 0.531420 8:-74755055 None:intergenic
AATCTAATATATTTGAGCAT+AGG 0.533676 8:-74755017 None:intergenic
AAGAGAAGGACTTGCTCAAG+AGG 0.534200 8:+74754571 MsG0880046549.01.T01:CDS
AATCATCCAAGGTGGAAGAA+TGG 0.535408 8:-74755337 None:intergenic
GCGAGACCATATAGTATGGC+CGG 0.548833 8:-74755570 None:intergenic
GTAGTATTCATGGATTCCAA+TGG 0.573152 8:-74754634 None:intergenic
TGAATTTCATTGTTGCTGGC+CGG 0.573310 8:+74755551 MsG0880046549.01.T01:three_prime_UTR
ACTATACACGGCCAGAGAGG+TGG 0.575565 8:-74754702 None:intergenic
TAACAACTTGCTCGCCAAGA+AGG 0.586073 8:+74754435 MsG0880046549.01.T01:CDS
CTATACACGGCCAGAGAGGT+GGG 0.592227 8:-74754701 None:intergenic
AGAGCATTAAATCTCTATAG+GGG 0.595391 8:-74754983 None:intergenic
GTGGTGCCATTCTTCCACCT+TGG 0.595581 8:+74755331 MsG0880046549.01.T01:three_prime_UTR
CTCTCAAACAAATTAGATCA+CGG 0.597625 8:+74752665 MsG0880046549.01.T01:CDS
TTTATAGGACGAATCATCCA+AGG 0.606737 8:-74755348 None:intergenic
CAAACAAATTAGATCACGGA+AGG 0.617902 8:+74752669 MsG0880046549.01.T01:CDS
AAGAGGAACTGCAAATAGTG+TGG 0.624610 8:+74754588 MsG0880046549.01.T01:CDS
ACAGCAGCACAAACAAGATG+TGG 0.645176 8:+74755312 MsG0880046549.01.T01:three_prime_UTR
GAGCATTAAATCTCTATAGG+GGG 0.677837 8:-74754982 None:intergenic
AGGAACTGCAAATAGTGTGG+AGG 0.678073 8:+74754591 MsG0880046549.01.T01:CDS
AGATGCATATGCACTATACA+CGG 0.680447 8:-74754714 None:intergenic
GGAAGAACTATATTAAGACA+TGG 0.692653 8:-74755198 None:intergenic
TGGAATCCATGAATACTACA+GGG 0.694406 8:+74754638 MsG0880046549.01.T01:CDS
TGCACTATACACGGCCAGAG+AGG 0.696503 8:-74754705 None:intergenic
TGTGCTATTAATTTGTCACA+TGG 0.709765 8:+74754934 MsG0880046549.01.T01:three_prime_UTR
ATAGGACGAATCATCCAAGG+TGG 0.747780 8:-74755345 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACTTGTTTTATAGTAAAATA+GGG + Chr8:74754199-74754218 MsG0880046549.01.T01:intron 15.0%
!!! TACTTGTTTTATAGTAAAAT+AGG + Chr8:74754198-74754217 MsG0880046549.01.T01:intron 15.0%
!! AAATAGATTCATACATAACT+TGG - Chr8:74752789-74752808 None:intergenic 20.0%
!! AATCTAATATATTTGAGCAT+AGG - Chr8:74755020-74755039 None:intergenic 20.0%
!! ATAGTATATCATCGATATAT+AGG + Chr8:74755467-74755486 MsG0880046549.01.T01:three_prime_UTR 20.0%
!! ATATTATTACATAAAAGTGG+AGG - Chr8:74754297-74754316 None:intergenic 20.0%
!! CACATATTATTACATAAAAG+TGG - Chr8:74754300-74754319 None:intergenic 20.0%
!! GATAGTCATACATTATAATT+TGG + Chr8:74754354-74754373 MsG0880046549.01.T01:intron 20.0%
!! TAAAACAACCTAAAAATTGT+TGG + Chr8:74754957-74754976 MsG0880046549.01.T01:three_prime_UTR 20.0%
!! TAAAATATGCATAGTTATTG+AGG - Chr8:74755120-74755139 None:intergenic 20.0%
!! TACATAACAAGCTTAAAATT+TGG + Chr8:74753052-74753071 MsG0880046549.01.T01:intron 20.0%
!! TACTATAAAACAAGTATGTT+TGG - Chr8:74754194-74754213 None:intergenic 20.0%
!! TATATGTTGCATATTCAAAT+AGG - Chr8:74755222-74755241 None:intergenic 20.0%
!! TCAAAAAAATAGTACACATT+AGG - Chr8:74754767-74754786 None:intergenic 20.0%
!! TGAATTATACTCACTTAATT+TGG + Chr8:74752749-74752768 MsG0880046549.01.T01:intron 20.0%
!! TGATGTATAATTGAATTCAA+AGG + Chr8:74754676-74754695 MsG0880046549.01.T01:exon 20.0%
!! TTATAAATAGAGCTAATTAG+AGG - Chr8:74755058-74755077 None:intergenic 20.0%
!!! TGTGTACTATTTTTTTGATT+TGG + Chr8:74754769-74754788 MsG0880046549.01.T01:three_prime_UTR 20.0%
!!! TTTAGTGAGAATCAATTTTA+CGG + Chr8:74754146-74754165 MsG0880046549.01.T01:intron 20.0%
! AATACAATCTGATTAAAGCT+CGG + Chr8:74753134-74753153 MsG0880046549.01.T01:intron 25.0%
! ATGCAATTTCTTGATGTTAT+CGG + Chr8:74753431-74753450 MsG0880046549.01.T01:intron 25.0%
! CGATGATATACTATATCTAT+AGG - Chr8:74755461-74755480 None:intergenic 25.0%
! GAGAGAGAATAAAATATTGT+AGG - Chr8:74754885-74754904 None:intergenic 25.0%
! TAATTACACAATTACAATGC+AGG - Chr8:74755516-74755535 None:intergenic 25.0%
! TATTTCAGAACTTCAGAAAA+AGG + Chr8:74752804-74752823 MsG0880046549.01.T01:intron 25.0%
! TTCTGCTACTATTCATTTAT+AGG - Chr8:74755366-74755385 None:intergenic 25.0%
! TTGAACCATTTGATAAAGAT+CGG + Chr8:74753208-74753227 MsG0880046549.01.T01:intron 25.0%
! TTTAGTACCAAAATTGTGTT+AGG - Chr8:74754017-74754036 None:intergenic 25.0%
!! AATTTTACGGAATCAATTCT+TGG + Chr8:74754159-74754178 MsG0880046549.01.T01:intron 25.0%
!! TAGGATTTTGCAATCAATTT+GGG + Chr8:74752958-74752977 MsG0880046549.01.T01:intron 25.0%
!!! AAGCTTGTTATGTAATTTGA+TGG - Chr8:74753046-74753065 None:intergenic 25.0%
!!! AGAATCAGTTCTAAAATCAA+AGG - Chr8:74754053-74754072 None:intergenic 25.0%
!!! ATTTTTTTGAGAAGTCAACT+TGG + Chr8:74753626-74753645 MsG0880046549.01.T01:intron 25.0%
!!! GTGTTTTGAATAAAGAAAAG+TGG - Chr8:74755419-74755438 None:intergenic 25.0%
AAAAAATGGTGCAAAAGATG+TGG + Chr8:74752924-74752943 MsG0880046549.01.T01:intron 30.0%
AAAACACAAATACAGCTTGT+AGG + Chr8:74755432-74755451 MsG0880046549.01.T01:three_prime_UTR 30.0%
AATATTGACCAAACACACAT+TGG + Chr8:74754332-74754351 MsG0880046549.01.T01:intron 30.0%
ACAATCCGATCTTTATCAAA+TGG - Chr8:74753216-74753235 None:intergenic 30.0%
AGAAAACATCATTACCTTCT+TGG - Chr8:74754452-74754471 None:intergenic 30.0%
ATAAAGGAAATGGCTACAAT+CGG - Chr8:74753827-74753846 None:intergenic 30.0%
ATCGATATATAGGCATATGA+TGG + Chr8:74755477-74755496 MsG0880046549.01.T01:three_prime_UTR 30.0%
ATTATCCGATCTTTACCAAA+TGG + Chr8:74753538-74753557 MsG0880046549.01.T01:intron 30.0%
CTACATTTGCACTATCATTA+GGG + Chr8:74754839-74754858 MsG0880046549.01.T01:three_prime_UTR 30.0%
CTCTCAAACAAATTAGATCA+CGG + Chr8:74752665-74752684 MsG0880046549.01.T01:CDS 30.0%
CTGCATGATATAGAAGAAAA+GGG - Chr8:74754395-74754414 None:intergenic 30.0%
GGAAGAACTATATTAAGACA+TGG - Chr8:74755201-74755220 None:intergenic 30.0%
GTCAATTTAATCACGCATTT+AGG - Chr8:74753248-74753267 None:intergenic 30.0%
GTTATCGGGATAATATATGT+CGG + Chr8:74753446-74753465 MsG0880046549.01.T01:intron 30.0%
TAGGCTTAAATCCTAACAAT+TGG - Chr8:74753914-74753933 None:intergenic 30.0%
TCTTCATTGCAATTCATGTT+GGG - Chr8:74755291-74755310 None:intergenic 30.0%
TGCAATTTCTTGATGTTATC+GGG + Chr8:74753432-74753451 MsG0880046549.01.T01:intron 30.0%
TGCAGAAAAATTGAATGTAG+TGG - Chr8:74754814-74754833 None:intergenic 30.0%
TGCAGATAAAGAAAAAGAGA+AGG + Chr8:74754557-74754576 MsG0880046549.01.T01:intron 30.0%
TTCTCTTTCCCTTGAAAAAA+TGG + Chr8:74752910-74752929 MsG0880046549.01.T01:intron 30.0%
TTCTTCATTGCAATTCATGT+TGG - Chr8:74755292-74755311 None:intergenic 30.0%
TTGGATACTCTTATATTCAC+TGG + Chr8:74754788-74754807 MsG0880046549.01.T01:three_prime_UTR 30.0%
! AGAGCATTAAATCTCTATAG+GGG - Chr8:74754986-74755005 None:intergenic 30.0%
! CTAGGATTTTGCAATCAATT+TGG + Chr8:74752957-74752976 MsG0880046549.01.T01:intron 30.0%
! GACACTTATAAAGCTTTTGA+TGG - Chr8:74753571-74753590 None:intergenic 30.0%
! TGCTACATTGAGAATACTTT+TGG + Chr8:74752705-74752724 MsG0880046549.01.T01:intron 30.0%
! TTCTCTTTTTCTTTATCTGC+AGG - Chr8:74754558-74754577 None:intergenic 30.0%
! TTTGTTTCAGGATTTTATGG+GGG + Chr8:74752582-74752601 MsG0880046549.01.T01:CDS 30.0%
!! CTTTGTTTCAGGATTTTATG+GGG + Chr8:74752581-74752600 MsG0880046549.01.T01:CDS 30.0%
!! GAGAGCATTAAATCTCTATA+GGG - Chr8:74754987-74755006 None:intergenic 30.0%
!! TGAGAGCATTAAATCTCTAT+AGG - Chr8:74754988-74755007 None:intergenic 30.0%
!! TGTGCTATTAATTTGTCACA+TGG + Chr8:74754934-74754953 MsG0880046549.01.T01:three_prime_UTR 30.0%
!!! CTTTTGCACCATTTTTTCAA+GGG - Chr8:74752921-74752940 None:intergenic 30.0%
!!! GCTTTGTTTCAGGATTTTAT+GGG + Chr8:74752580-74752599 MsG0880046549.01.T01:CDS 30.0%
!!! TCTTTTGCACCATTTTTTCA+AGG - Chr8:74752922-74752941 None:intergenic 30.0%
!!! TGCTTTGTTTCAGGATTTTA+TGG + Chr8:74752579-74752598 MsG0880046549.01.T01:CDS 30.0%
AATGTCAAACATAAGTCGTG+AGG - Chr8:74753660-74753679 None:intergenic 35.0%
AATGTCGAAAAGTGATCAGA+CGG + Chr8:74753005-74753024 MsG0880046549.01.T01:intron 35.0%
AGATGCATATGCACTATACA+CGG - Chr8:74754717-74754736 None:intergenic 35.0%
AGTAAAATAGGGACGAACAT+TGG + Chr8:74754210-74754229 MsG0880046549.01.T01:intron 35.0%
AGTTGAAGGTAGAGTGATTT+GGG - Chr8:74754122-74754141 None:intergenic 35.0%
ATGACTATCCAATGTGTGTT+TGG - Chr8:74754343-74754362 None:intergenic 35.0%
ATTGAGAGTTGGACACAAAA+GGG - Chr8:74753884-74753903 None:intergenic 35.0%
CAAACAAATTAGATCACGGA+AGG + Chr8:74752669-74752688 MsG0880046549.01.T01:CDS 35.0%
CAAGTTGTTATGTTCTTGGA+GGG - Chr8:74754425-74754444 None:intergenic 35.0%
CACTTTAATCTACACGATGT+GGG + Chr8:74753734-74753753 MsG0880046549.01.T01:intron 35.0%
CACTTTAATCTACTCGATGT+GGG + Chr8:74753988-74754007 MsG0880046549.01.T01:intron 35.0%
CATTCCAAATGCCAAAATCT+TGG + Chr8:74755080-74755099 MsG0880046549.01.T01:three_prime_UTR 35.0%
CCTACATTTGCACTATCATT+AGG + Chr8:74754838-74754857 MsG0880046549.01.T01:three_prime_UTR 35.0%
CCTGCATGATATAGAAGAAA+AGG - Chr8:74754396-74754415 None:intergenic 35.0%
CTCAACCATTTGGTAAAGAT+CGG - Chr8:74753546-74753565 None:intergenic 35.0%
CTTCTATATCATGCAGGATA+AGG + Chr8:74754399-74754418 MsG0880046549.01.T01:intron 35.0%
GAGCATTAAATCTCTATAGG+GGG - Chr8:74754985-74755004 None:intergenic 35.0%
GCATGTGTATACAAACGATA+GGG + Chr8:74752868-74752887 MsG0880046549.01.T01:intron 35.0%
GTAGTATTCATGGATTCCAA+TGG - Chr8:74754637-74754656 None:intergenic 35.0%
TAAAGGAAATGGCTACAATC+GGG - Chr8:74753826-74753845 None:intergenic 35.0%
TATAGCGAGACCATATAGTA+TGG - Chr8:74755577-74755596 None:intergenic 35.0%
TCAAGTTTCTCTAGTAGTGT+AGG - Chr8:74753102-74753121 None:intergenic 35.0%
TCCAATGGTTGAGTTTCAAT+TGG - Chr8:74754622-74754641 None:intergenic 35.0%
TCCAATTGAAACTCAACCAT+TGG + Chr8:74754618-74754637 MsG0880046549.01.T01:CDS 35.0%
TCTCAAAGAGCTTCAAAGTT+TGG + Chr8:74752624-74752643 MsG0880046549.01.T01:CDS 35.0%
TGACTGAATTTCATTGTTGC+TGG + Chr8:74755547-74755566 MsG0880046549.01.T01:three_prime_UTR 35.0%
TGCATGTGTATACAAACGAT+AGG + Chr8:74752867-74752886 MsG0880046549.01.T01:intron 35.0%
TGGAATCCATGAATACTACA+GGG + Chr8:74754638-74754657 MsG0880046549.01.T01:CDS 35.0%
TGTTAGGATTTAAGCCTATG+AGG + Chr8:74753916-74753935 MsG0880046549.01.T01:intron 35.0%
TTGGAATCCATGAATACTAC+AGG + Chr8:74754637-74754656 MsG0880046549.01.T01:CDS 35.0%
TTTATAGGACGAATCATCCA+AGG - Chr8:74755351-74755370 None:intergenic 35.0%
! AGCAAAGCGTTTATCTCTAT+AGG - Chr8:74754909-74754928 None:intergenic 35.0%
! ATCGAGTAGATTAAAGTGCT+TGG - Chr8:74753986-74754005 None:intergenic 35.0%
! CAATGACTAGTCCATTAAGA+GGG + Chr8:74753950-74753969 MsG0880046549.01.T01:intron 35.0%
! CCTAATGATAGTGCAAATGT+AGG - Chr8:74754841-74754860 None:intergenic 35.0%
! CCTTTTCTTCTATATCATGC+AGG + Chr8:74754393-74754412 MsG0880046549.01.T01:intron 35.0%
! GTAGATTAAAGTGCTTGATG+TGG - Chr8:74753727-74753746 None:intergenic 35.0%
! TCTCACTAAAAGTGAGTTGA+AGG - Chr8:74754136-74754155 None:intergenic 35.0%
! TTTGATGGATCTCAACCATT+TGG - Chr8:74753556-74753575 None:intergenic 35.0%
! TTTTAACACTATGCCGAATG+TGG + Chr8:74754481-74754500 MsG0880046549.01.T01:intron 35.0%
!! CAAGATTTTGGCATTTGGAA+TGG - Chr8:74755082-74755101 None:intergenic 35.0%
!! CAATGAAAGCACCAATTGTT+AGG + Chr8:74753900-74753919 MsG0880046549.01.T01:intron 35.0%
!! CTCTTAATGGACTAGTCATT+GGG - Chr8:74753951-74753970 None:intergenic 35.0%
!! TCTTAATGGACTAGTCATTG+GGG - Chr8:74753950-74753969 None:intergenic 35.0%
!! TTTTTGAGAAGTCAACTTGG+TGG + Chr8:74753629-74753648 MsG0880046549.01.T01:intron 35.0%
!!! TGAGAATACTTTTGGTGATG+TGG + Chr8:74752713-74752732 MsG0880046549.01.T01:intron 35.0%
AAGAGGAACTGCAAATAGTG+TGG + Chr8:74754588-74754607 MsG0880046549.01.T01:CDS 40.0%
AATCATCCAAGGTGGAAGAA+TGG - Chr8:74755340-74755359 None:intergenic 40.0%
AGATGTGGTAGATGGTTTCT+AGG + Chr8:74752939-74752958 MsG0880046549.01.T01:intron 40.0%
ATGTCGAAAAGTGATCAGAC+GGG + Chr8:74753006-74753025 MsG0880046549.01.T01:intron 40.0%
CACTAACCCTGTAGTATTCA+TGG - Chr8:74754647-74754666 None:intergenic 40.0%
CATTGAGAGTTGGACACAAA+AGG - Chr8:74753885-74753904 None:intergenic 40.0%
CCAAATCCAAACACCACATT+CGG - Chr8:74754497-74754516 None:intergenic 40.0%
CCAACTTCATTAGCTCGATT+CGG + Chr8:74753278-74753297 MsG0880046549.01.T01:intron 40.0%
CGAGCAAGTTGTTATGTTCT+TGG - Chr8:74754429-74754448 None:intergenic 40.0%
GAGTTGAAGGTAGAGTGATT+TGG - Chr8:74754123-74754142 None:intergenic 40.0%
GATGTGGTATTTAAGTCGTG+AGG - Chr8:74753711-74753730 None:intergenic 40.0%
GCAAGTTGTTATGTTCTTGG+AGG - Chr8:74754426-74754445 None:intergenic 40.0%
GCACTTTAATCTACACGATG+TGG + Chr8:74753733-74753752 MsG0880046549.01.T01:intron 40.0%
GCACTTTAATCTACTCGATG+TGG + Chr8:74753987-74754006 MsG0880046549.01.T01:intron 40.0%
GCGTGATTAAATTGACTGCT+AGG + Chr8:74753252-74753271 MsG0880046549.01.T01:intron 40.0%
TCAACTTCGTTAGCTCGATT+AGG + Chr8:74753499-74753518 MsG0880046549.01.T01:intron 40.0%
TCCAAATGCCAAAATCTTGG+TGG + Chr8:74755083-74755102 MsG0880046549.01.T01:three_prime_UTR 40.0%
TGAAGCGGTCATAAAGGAAA+TGG - Chr8:74753837-74753856 None:intergenic 40.0%
TGAATTTCATTGTTGCTGGC+CGG + Chr8:74755551-74755570 MsG0880046549.01.T01:three_prime_UTR 40.0%
! AAGGTAGAGTGATTTGGGTT+TGG - Chr8:74754117-74754136 None:intergenic 40.0%
! AGCTCGGGATAATTTTCATC+GGG - Chr8:74753333-74753352 None:intergenic 40.0%
! CAGCTCGGGATAATTTTCAT+CGG - Chr8:74753334-74753353 None:intergenic 40.0%
! CCAATGACTAGTCCATTAAG+AGG + Chr8:74753949-74753968 MsG0880046549.01.T01:intron 40.0%
! GATCAGACGGGATCATATTT+TGG + Chr8:74753018-74753037 MsG0880046549.01.T01:intron 40.0%
! GGAGGGTGTGGTTATAATTT+AGG + Chr8:74753404-74753423 MsG0880046549.01.T01:intron 40.0%
! GTAGATTAAAGTGCTTGGTG+TGG - Chr8:74753981-74754000 None:intergenic 40.0%
! GTTACAAGCCACCAAGATTT+TGG - Chr8:74755094-74755113 None:intergenic 40.0%
! TGGGACTCCTAACACAATTT+TGG + Chr8:74754007-74754026 MsG0880046549.01.T01:intron 40.0%
! TTGGTGCTTTCATTGAGAGT+TGG - Chr8:74753895-74753914 None:intergenic 40.0%
!! CCTCTTAATGGACTAGTCAT+TGG - Chr8:74753952-74753971 None:intergenic 40.0%
!! TACTGAGTTGATGCAGATCT+GGG + Chr8:74755264-74755283 MsG0880046549.01.T01:three_prime_UTR 40.0%
!! TTACTGAGTTGATGCAGATC+TGG + Chr8:74755263-74755282 MsG0880046549.01.T01:three_prime_UTR 40.0%
!!! AGGGGGTACCAACAATTTTT+AGG - Chr8:74754968-74754987 None:intergenic 40.0%
ACAGCAGCACAAACAAGATG+TGG + Chr8:74755312-74755331 MsG0880046549.01.T01:three_prime_UTR 45.0%
ACTATGCCGAATGTGGTGTT+TGG + Chr8:74754488-74754507 MsG0880046549.01.T01:intron 45.0%
AGGAACTGCAAATAGTGTGG+AGG + Chr8:74754591-74754610 MsG0880046549.01.T01:CDS 45.0%
ATAGGACGAATCATCCAAGG+TGG - Chr8:74755348-74755367 None:intergenic 45.0%
CACGACGACTTTCACTCTAT+CGG + Chr8:74753794-74753813 MsG0880046549.01.T01:intron 45.0%
CCGAATCGAGCTAATGAAGT+TGG - Chr8:74753281-74753300 None:intergenic 45.0%
CCGAATGTGGTGTTTGGATT+TGG + Chr8:74754494-74754513 MsG0880046549.01.T01:intron 45.0%
GGGCTACACGAATTACCAAT+AGG - Chr8:74753864-74753883 None:intergenic 45.0%
GGTGCAAAAGATGTGGTAGA+TGG + Chr8:74752931-74752950 MsG0880046549.01.T01:intron 45.0%
TTACCAATAGGCGAGTGAAG+CGG - Chr8:74753852-74753871 None:intergenic 45.0%
TTTATGGGGGAAGACTTGGA+TGG + Chr8:74752595-74752614 MsG0880046549.01.T01:CDS 45.0%
! AAGAGAAGGACTTGCTCAAG+AGG + Chr8:74754571-74754590 MsG0880046549.01.T01:CDS 45.0%
! GGATTTTATGGGGGAAGACT+TGG + Chr8:74752591-74752610 MsG0880046549.01.T01:CDS 45.0%
! TAACAACTTGCTCGCCAAGA+AGG + Chr8:74754435-74754454 MsG0880046549.01.T01:CDS 45.0%
! TGTCCAGTTAAGTTGCTCCT+TGG + Chr8:74753369-74753388 MsG0880046549.01.T01:intron 45.0%
!! GCCACCAAGATTTTGGCATT+TGG - Chr8:74755087-74755106 None:intergenic 45.0%
CGATAGAGTGAAAGTCGTCG+TGG - Chr8:74753796-74753815 None:intergenic 50.0%
GCGAGACCATATAGTATGGC+CGG - Chr8:74755573-74755592 None:intergenic 50.0%
GGGGAAGACTTGGATGGTTT+AGG + Chr8:74752601-74752620 MsG0880046549.01.T01:CDS 50.0%
GTGTGGTGACTCCCTCTTAA+TGG - Chr8:74753964-74753983 None:intergenic 50.0%
TTCAAAGGTTCCCACCTCTC+TGG + Chr8:74754691-74754710 MsG0880046549.01.T01:three_prime_UTR 50.0%
! CAGTTAAGTTGCTCCTTGGC+CGG + Chr8:74753373-74753392 MsG0880046549.01.T01:intron 50.0%
! TTGTCACGCCCTTTAAGAGC+CGG + Chr8:74753767-74753786 MsG0880046549.01.T01:intron 50.0%
ACTATACACGGCCAGAGAGG+TGG - Chr8:74754705-74754724 None:intergenic 55.0%
ATCCCGAGCTGATCACGAAG+AGG + Chr8:74753343-74753362 MsG0880046549.01.T01:intron 55.0%
CACCTCTTCGTGATCAGCTC+GGG - Chr8:74753348-74753367 None:intergenic 55.0%
CCACACCCTCCCTTGAATTC+CGG - Chr8:74753395-74753414 None:intergenic 55.0%
CGGCCAAGGAGCAACTTAAC+TGG - Chr8:74753375-74753394 None:intergenic 55.0%
CTATACACGGCCAGAGAGGT+GGG - Chr8:74754704-74754723 None:intergenic 55.0%
CTTGGCCGGAATTCAAGGGA+GGG + Chr8:74753387-74753406 MsG0880046549.01.T01:intron 55.0%
GCACCTCTTCGTGATCAGCT+CGG - Chr8:74753349-74753368 None:intergenic 55.0%
GCTCCTTGGCCGGAATTCAA+GGG + Chr8:74753383-74753402 MsG0880046549.01.T01:intron 55.0%
GGCGAGTGAAGCGGTCATAA+AGG - Chr8:74753843-74753862 None:intergenic 55.0%
GTGAAAGTCGTCGTGGACAC+CGG - Chr8:74753789-74753808 None:intergenic 55.0%
TGACCGCTTCACTCGCCTAT+TGG + Chr8:74753846-74753865 MsG0880046549.01.T01:intron 55.0%
TGCACTATACACGGCCAGAG+AGG - Chr8:74754708-74754727 None:intergenic 55.0%
TGCTCCTTGGCCGGAATTCA+AGG + Chr8:74753382-74753401 MsG0880046549.01.T01:intron 55.0%
TGCTGGCCGGCCATACTATA+TGG + Chr8:74755564-74755583 MsG0880046549.01.T01:three_prime_UTR 55.0%
TTGGGGTGAGCTCTCCTCAT+AGG - Chr8:74753933-74753952 None:intergenic 55.0%
!! CGTGGACACCGGCTCTTAAA+GGG - Chr8:74753778-74753797 None:intergenic 55.0%
!! GTGGTGCCATTCTTCCACCT+TGG + Chr8:74755331-74755350 MsG0880046549.01.T01:three_prime_UTR 55.0%
!! TCGTGGACACCGGCTCTTAA+AGG - Chr8:74753779-74753798 None:intergenic 55.0%
CCGGAATTCAAGGGAGGGTG+TGG + Chr8:74753392-74753411 MsG0880046549.01.T01:intron 60.0%
CCTCCCTTGAATTCCGGCCA+AGG - Chr8:74753389-74753408 None:intergenic 60.0%
CCTTGGCCGGAATTCAAGGG+AGG + Chr8:74753386-74753405 MsG0880046549.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr8 gene 74752571 74755615 74752571 ID=MsG0880046549.01;Name=MsG0880046549.01
Chr8 mRNA 74752571 74755615 74752571 ID=MsG0880046549.01.T01;Parent=MsG0880046549.01;Name=MsG0880046549.01.T01;_AED=0.37;_eAED=0.37;_QI=0|0.5|0.66|1|0|0|3|937|92
Chr8 exon 74752571 74752690 74752571 ID=MsG0880046549.01.T01:exon:31275;Parent=MsG0880046549.01.T01
Chr8 exon 74754415 74754456 74754415 ID=MsG0880046549.01.T01:exon:31276;Parent=MsG0880046549.01.T01
Chr8 exon 74754562 74755615 74754562 ID=MsG0880046549.01.T01:exon:31277;Parent=MsG0880046549.01.T01
Chr8 CDS 74752571 74752690 74752571 ID=MsG0880046549.01.T01:cds;Parent=MsG0880046549.01.T01
Chr8 CDS 74754415 74754456 74754415 ID=MsG0880046549.01.T01:cds;Parent=MsG0880046549.01.T01
Chr8 CDS 74754562 74754678 74754562 ID=MsG0880046549.01.T01:cds;Parent=MsG0880046549.01.T01
Chr8 three_prime_UTR 74754679 74755615 74754679 ID=MsG0880046549.01.T01:three_prime_utr;Parent=MsG0880046549.01.T01
Gene Sequence

>MsG0880046549.01.T01

ATGGCAACTGCTTTGTTTCAGGATTTTATGGGGGAAGACTTGGATGGTTTAGGTCTCAAAGAGCTTCAAAGTTTGGAGCAACAACTTGATTCAGCTCTCAAACAAATTAGATCACGGAAGGATAAGGCCCTCCAAGAACATAACAACTTGCTCGCCAAGAAGATAAAGAAAAAGAGAAGGACTTGCTCAAGAGGAACTGCAAATAGTGTGGAGGTGACTCCAATTGAAACTCAACCATTGGAATCCATGAATACTACAGGGTTAGTGTATTTTCTTTGA
Protein sequence

>MsG0880046549.01.T01

MATALFQDFMGEDLDGLGLKELQSLEQQLDSALKQIRSRKDKALQEHNNLLAKKIKKKRRTCSRGTANSVEVTPIETQPLESMNTTGLVYFL*