AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880046704.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046704.01.T01 MTR_4g113270 99.133 346 2 1 1 346 1 345 0.0 681
MsG0880046704.01.T01 MTR_4g113270 99.118 340 2 1 1 340 1 339 0.0 670
MsG0880046704.01.T01 MTR_1g100860 50.158 317 149 3 15 328 41 351 3.76e-98 295
MsG0880046704.01.T01 MTR_4g018770 49.527 317 151 3 15 328 35 345 4.61e-98 295
MsG0880046704.01.T01 MTR_4g018770 49.412 255 120 3 77 328 5 253 2.26e-76 236
MsG0880046704.01.T01 MTR_1g100860 49.804 255 119 3 77 328 5 253 3.06e-76 236
MsG0880046704.01.T01 MTR_3g463360 24.242 297 206 6 19 304 108 396 9.78e-13 68.9
MsG0880046704.01.T01 MTR_3g109070 27.692 260 164 7 18 267 20 265 2.18e-12 67.4
MsG0880046704.01.T01 MTR_3g109070 27.692 260 164 7 18 267 20 265 3.44e-12 66.2
MsG0880046704.01.T01 MTR_3g096310 23.102 303 218 5 18 311 45 341 6.90e-12 66.2
MsG0880046704.01.T01 MTR_3g109070 28.218 202 126 5 18 210 20 211 2.98e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046704.01.T01 AT5G19980 71.470 347 91 3 1 346 1 340 1.73e-169 475
MsG0880046704.01.T01 AT1G76340 48.148 324 166 2 15 337 37 359 8.55e-100 300
MsG0880046704.01.T01 AT4G31600 26.848 257 170 5 18 267 19 264 4.45e-13 69.7
MsG0880046704.01.T01 AT2G13650 22.896 297 212 5 18 304 38 327 8.98e-12 65.9
MsG0880046704.01.T01 AT2G13650 22.896 297 212 5 18 304 38 327 8.98e-12 65.9
MsG0880046704.01.T01 AT4G31600 27.136 199 132 3 18 210 19 210 1.32e-11 65.1

Find 62 sgRNAs with CRISPR-Local

Find 79 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTTGGTTGTTATTCTTGC+TGG 0.233454 8:-77033496 MsG0880046704.01.T01:CDS
GCTACCGCGTTTACCGTTAC+TGG 0.269983 8:-77033130 MsG0880046704.01.T01:CDS
GGTTTGTTGATTAGTTTCTT+TGG 0.270396 8:-77033178 MsG0880046704.01.T01:CDS
GAAACCCTAATTTACCTAAA+AGG 0.287655 8:+77033728 None:intergenic
TTACACTCACGGCTTATTCT+TGG 0.301434 8:-77033441 MsG0880046704.01.T01:CDS
AATAACAACCAAGGACAAAA+AGG 0.342091 8:+77033504 None:intergenic
TACACTCACGGCTTATTCTT+GGG 0.343245 8:-77033440 MsG0880046704.01.T01:CDS
GGTTATGTTGCTACTGATTC+CGG 0.348332 8:-77033466 MsG0880046704.01.T01:CDS
CTACCGCGTTTACCGTTACT+GGG 0.359242 8:-77033129 MsG0880046704.01.T01:CDS
TGGCAATTAATGTGACGATT+TGG 0.360897 8:-77033084 MsG0880046704.01.T01:CDS
GTTTCGTTGTCTTGTGTGTT+TGG 0.368137 8:-77033199 MsG0880046704.01.T01:CDS
TGAGTCTTGGATTGAATACT+TGG 0.371617 8:-77033360 MsG0880046704.01.T01:CDS
GATGGTTTATATTAAGCATA+TGG 0.374607 8:-77033386 MsG0880046704.01.T01:CDS
GCATGCTAGTCCAGCTGGTT+TGG 0.378044 8:-77033056 MsG0880046704.01.T01:CDS
AAGCATATGGTTATGAGTCT+TGG 0.400403 8:-77033373 MsG0880046704.01.T01:CDS
GGCAATTAATGTGACGATTT+GGG 0.402674 8:-77033083 MsG0880046704.01.T01:CDS
GACTGGAAGTGGTTCGCAAC+AGG 0.414839 8:-77032984 MsG0880046704.01.T01:CDS
GTTATTCTTGCTGGTGCTGT+TGG 0.419854 8:-77033487 MsG0880046704.01.T01:CDS
GATGTTGAAAGTAGTCTTGT+TGG 0.433983 8:-77032935 MsG0880046704.01.T01:CDS
GCCACAAGTGGCTTAGTAAT+CGG 0.437849 8:-77033865 MsG0880046704.01.T01:CDS
GGGAAGAATTTCTTAGCGAT+TGG 0.443284 8:+77033682 None:intergenic
AAGAAGAAAACGAGTGCTGC+AGG 0.450306 8:+77033661 None:intergenic
CAGCAATCGGTGACTGGAAG+TGG 0.465887 8:-77032995 MsG0880046704.01.T01:CDS
GGGTGTTCTTTATCAGCAAT+CGG 0.471127 8:-77033008 MsG0880046704.01.T01:CDS
CTTTATCAGCAATCGGTGAC+TGG 0.474553 8:-77033001 MsG0880046704.01.T01:CDS
TGGTTCGCAACAGGCGGTTG+TGG 0.476669 8:-77032975 MsG0880046704.01.T01:CDS
GTTAGCGTGACGAAGGAGAT+TGG 0.477146 8:+77033621 None:intergenic
ATAAGCCGTGAGTGTAAAAC+CGG 0.495535 8:+77033447 None:intergenic
ACTGCCTTGAGATCGAGTAC+TGG 0.496102 8:-77033253 MsG0880046704.01.T01:CDS
TGAAGATGAGAGTGAAGGAA+AGG 0.502584 8:-77032900 MsG0880046704.01.T01:CDS
CAATATCTCACTTCTTCACT+CGG 0.505402 8:-77033757 MsG0880046704.01.T01:CDS
CTGCCTTGAGATCGAGTACT+GGG 0.507997 8:-77033252 MsG0880046704.01.T01:CDS
GATAAGCATGCTAGTCCAGC+TGG 0.512847 8:-77033061 MsG0880046704.01.T01:CDS
CTCGCTGATACTGCGTTTCG+CGG 0.516331 8:-77033550 MsG0880046704.01.T01:CDS
ATAAACCATCTCAGTAGTAA+TGG 0.529935 8:+77033399 None:intergenic
GAAGATGAGAGTGAAGGAAA+GGG 0.531374 8:-77032899 MsG0880046704.01.T01:CDS
AGCAAACAAACCAAACCAGC+TGG 0.537823 8:+77033046 None:intergenic
TTAGCGATTGGAATAGTGAA+AGG 0.542673 8:+77033694 None:intergenic
AGTCTTGGATTGAATACTTG+GGG 0.551343 8:-77033358 MsG0880046704.01.T01:CDS
GGTTACCATTACTACTGAGA+TGG 0.551400 8:-77033404 MsG0880046704.01.T01:CDS
GAGTCTTGGATTGAATACTT+GGG 0.552814 8:-77033359 MsG0880046704.01.T01:CDS
TGTAACGCAGTTAAAAGACC+TGG 0.556729 8:+77033778 None:intergenic
CAGCGAGAGCAACAAGAAGA+GGG 0.556974 8:+77033566 None:intergenic
TCAGCGAGAGCAACAAGAAG+AGG 0.569697 8:+77033565 None:intergenic
AAGGTAAAATTAGACGGAGA+AGG 0.587179 8:+77033523 None:intergenic
CAACAACACTACGCCACAAG+TGG 0.587960 8:-77033877 MsG0880046704.01.T01:CDS
GAAAGTAGTCTTGTTGGTGA+TGG 0.589182 8:-77032929 MsG0880046704.01.T01:CDS
ACCAGCAAGAATAACAACCA+AGG 0.595444 8:+77033495 None:intergenic
CTTATTCACAACCCCAGTAA+CGG 0.607538 8:+77033117 None:intergenic
GTGACGAAGGAGATTGGTGT+TGG 0.632002 8:+77033627 None:intergenic
ACTCCCAGTACTCGATCTCA+AGG 0.635928 8:+77033249 None:intergenic
ACCGATTACTAAGCCACTTG+TGG 0.637733 8:+77033864 None:intergenic
TATCAACGTTAGCGTGACGA+AGG 0.639770 8:+77033614 None:intergenic
ATTACTCAATTCAACTACCC+AGG 0.649152 8:-77033796 MsG0880046704.01.T01:CDS
GTAACGCAGTTAAAAGACCT+GGG 0.655430 8:+77033779 None:intergenic
AGAAGAAAACGAGTGCTGCA+GGG 0.656660 8:+77033662 None:intergenic
TGGAAGTGGTTCGCAACAGG+CGG 0.657769 8:-77032981 MsG0880046704.01.T01:CDS
AGCGAGAGCAACAAGAAGAG+GGG 0.662395 8:+77033567 None:intergenic
TGTGAATAAGTTTCTGACTG+TGG 0.688581 8:-77033104 MsG0880046704.01.T01:CDS
GATTCTGAAGATGAGAGTGA+AGG 0.689840 8:-77032905 MsG0880046704.01.T01:CDS
TACCGCGTTTACCGTTACTG+GGG 0.705827 8:-77033128 MsG0880046704.01.T01:CDS
AACCCCAGTAACGGTAAACG+CGG 0.739549 8:+77033126 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTACCTTTTAGGTAAATTA+GGG - Chr8:77033046-77033065 MsG0880046704.01.T01:CDS 20.0%
! GACAAAAAGGTAAAATTAGA+CGG + Chr8:77033264-77033283 None:intergenic 25.0%
! GATGGTTTATATTAAGCATA+TGG - Chr8:77033392-77033411 MsG0880046704.01.T01:CDS 25.0%
!! TAATTTTACCTTTTTGTCCT+TGG - Chr8:77033266-77033285 MsG0880046704.01.T01:CDS 25.0%
!!! GTTTACCTTTTAGGTAAATT+AGG - Chr8:77033045-77033064 MsG0880046704.01.T01:CDS 25.0%
!!! GTTTGCTTTTTACGATTATT+GGG - Chr8:77033748-77033767 MsG0880046704.01.T01:CDS 25.0%
!!! TGTTTGCTTTTTACGATTAT+TGG - Chr8:77033747-77033766 MsG0880046704.01.T01:CDS 25.0%
!!! TTTGCTTTTTACGATTATTG+GGG - Chr8:77033749-77033768 MsG0880046704.01.T01:CDS 25.0%
AATAACAACCAAGGACAAAA+AGG + Chr8:77033277-77033296 None:intergenic 30.0%
ATAAACCATCTCAGTAGTAA+TGG + Chr8:77033382-77033401 None:intergenic 30.0%
GAAACCCTAATTTACCTAAA+AGG + Chr8:77033053-77033072 None:intergenic 30.0%
!! GGTTTGTTGATTAGTTTCTT+TGG - Chr8:77033600-77033619 MsG0880046704.01.T01:CDS 30.0%
!!! TTGCTTTTTACGATTATTGG+GGG - Chr8:77033750-77033769 MsG0880046704.01.T01:CDS 30.0%
AAGCATATGGTTATGAGTCT+TGG - Chr8:77033405-77033424 MsG0880046704.01.T01:CDS 35.0%
AAGGTAAAATTAGACGGAGA+AGG + Chr8:77033258-77033277 None:intergenic 35.0%
AGTCTTGGATTGAATACTTG+GGG - Chr8:77033420-77033439 MsG0880046704.01.T01:CDS 35.0%
ATTACTCAATTCAACTACCC+AGG - Chr8:77032982-77033001 MsG0880046704.01.T01:CDS 35.0%
CAATATCTCACTTCTTCACT+CGG - Chr8:77033021-77033040 MsG0880046704.01.T01:CDS 35.0%
CCAGTCAAAAACCAAAAAAC+AGG + Chr8:77033486-77033505 None:intergenic 35.0%
GAGTCTTGGATTGAATACTT+GGG - Chr8:77033419-77033438 MsG0880046704.01.T01:CDS 35.0%
GGCAATTAATGTGACGATTT+GGG - Chr8:77033695-77033714 MsG0880046704.01.T01:CDS 35.0%
TGAGTCTTGGATTGAATACT+TGG - Chr8:77033418-77033437 MsG0880046704.01.T01:CDS 35.0%
TGGCAATTAATGTGACGATT+TGG - Chr8:77033694-77033713 MsG0880046704.01.T01:CDS 35.0%
TGTGAATAAGTTTCTGACTG+TGG - Chr8:77033674-77033693 MsG0880046704.01.T01:CDS 35.0%
TTAGCGATTGGAATAGTGAA+AGG + Chr8:77033087-77033106 None:intergenic 35.0%
! TTCTTGGGCTTTTGCTTATT+TGG - Chr8:77033353-77033372 MsG0880046704.01.T01:CDS 35.0%
! TTTGACTGGTGAGAATTTTG+AGG - Chr8:77033497-77033516 MsG0880046704.01.T01:CDS 35.0%
!! GATGTTGAAAGTAGTCTTGT+TGG - Chr8:77033843-77033862 MsG0880046704.01.T01:CDS 35.0%
!!! CCTGTTTTTTGGTTTTTGAC+TGG - Chr8:77033483-77033502 MsG0880046704.01.T01:CDS 35.0%
!!! TGATGATTGCTCCTGTTTTT+TGG - Chr8:77033472-77033491 MsG0880046704.01.T01:CDS 35.0%
ACCAGCAAGAATAACAACCA+AGG + Chr8:77033286-77033305 None:intergenic 40.0%
ATAAGCCGTGAGTGTAAAAC+CGG + Chr8:77033334-77033353 None:intergenic 40.0%
CTTATTCACAACCCCAGTAA+CGG + Chr8:77033664-77033683 None:intergenic 40.0%
GAAGATGAGAGTGAAGGAAA+GGG - Chr8:77033879-77033898 MsG0880046704.01.T01:CDS 40.0%
GGGAAGAATTTCTTAGCGAT+TGG + Chr8:77033099-77033118 None:intergenic 40.0%
GGGTGTTCTTTATCAGCAAT+CGG - Chr8:77033770-77033789 MsG0880046704.01.T01:CDS 40.0%
GGTTACCATTACTACTGAGA+TGG - Chr8:77033374-77033393 MsG0880046704.01.T01:CDS 40.0%
GTAACGCAGTTAAAAGACCT+GGG + Chr8:77033002-77033021 None:intergenic 40.0%
GTTTCGTTGTCTTGTGTGTT+TGG - Chr8:77033579-77033598 MsG0880046704.01.T01:CDS 40.0%
TACACTCACGGCTTATTCTT+GGG - Chr8:77033338-77033357 MsG0880046704.01.T01:CDS 40.0%
TGAAGATGAGAGTGAAGGAA+AGG - Chr8:77033878-77033897 MsG0880046704.01.T01:CDS 40.0%
TGTAACGCAGTTAAAAGACC+TGG + Chr8:77033003-77033022 None:intergenic 40.0%
TTACACTCACGGCTTATTCT+TGG - Chr8:77033337-77033356 MsG0880046704.01.T01:CDS 40.0%
! GATTCTGAAGATGAGAGTGA+AGG - Chr8:77033873-77033892 MsG0880046704.01.T01:CDS 40.0%
! TCCTTGGTTGTTATTCTTGC+TGG - Chr8:77033282-77033301 MsG0880046704.01.T01:CDS 40.0%
! TGATTCCGGTTTTACACTCA+CGG - Chr8:77033326-77033345 MsG0880046704.01.T01:CDS 40.0%
!! GAAAGTAGTCTTGTTGGTGA+TGG - Chr8:77033849-77033868 MsG0880046704.01.T01:CDS 40.0%
!! GGTTATGTTGCTACTGATTC+CGG - Chr8:77033312-77033331 MsG0880046704.01.T01:CDS 40.0%
!!! TCACTCGGTGTTTACCTTTT+AGG - Chr8:77033036-77033055 MsG0880046704.01.T01:CDS 40.0%
ACCGATTACTAAGCCACTTG+TGG + Chr8:77032917-77032936 None:intergenic 45.0%
AGCAAACAAACCAAACCAGC+TGG + Chr8:77033735-77033754 None:intergenic 45.0%
CTTTATCAGCAATCGGTGAC+TGG - Chr8:77033777-77033796 MsG0880046704.01.T01:CDS 45.0%
GCCACAAGTGGCTTAGTAAT+CGG - Chr8:77032913-77032932 MsG0880046704.01.T01:CDS 45.0%
TATCAACGTTAGCGTGACGA+AGG + Chr8:77033167-77033186 None:intergenic 45.0%
! AAGAAGAAAACGAGTGCTGC+AGG + Chr8:77033120-77033139 None:intergenic 45.0%
! AGAAGAAAACGAGTGCTGCA+GGG + Chr8:77033119-77033138 None:intergenic 45.0%
! GTTCGACGTGAATGCGTTTT+TGG - Chr8:77033554-77033573 MsG0880046704.01.T01:CDS 45.0%
!! GTTATTCTTGCTGGTGCTGT+TGG - Chr8:77033291-77033310 MsG0880046704.01.T01:CDS 45.0%
AACCCCAGTAACGGTAAACG+CGG + Chr8:77033655-77033674 None:intergenic 50.0%
ACTCCCAGTACTCGATCTCA+AGG + Chr8:77033532-77033551 None:intergenic 50.0%
ACTGCCTTGAGATCGAGTAC+TGG - Chr8:77033525-77033544 MsG0880046704.01.T01:CDS 50.0%
AGCGAGAGCAACAAGAAGAG+GGG + Chr8:77033214-77033233 None:intergenic 50.0%
CAACAACACTACGCCACAAG+TGG - Chr8:77032901-77032920 MsG0880046704.01.T01:CDS 50.0%
CAGCGAGAGCAACAAGAAGA+GGG + Chr8:77033215-77033234 None:intergenic 50.0%
CTACCGCGTTTACCGTTACT+GGG - Chr8:77033649-77033668 MsG0880046704.01.T01:CDS 50.0%
CTGCCTTGAGATCGAGTACT+GGG - Chr8:77033526-77033545 MsG0880046704.01.T01:CDS 50.0%
GATAAGCATGCTAGTCCAGC+TGG - Chr8:77033717-77033736 MsG0880046704.01.T01:CDS 50.0%
GTGACGAAGGAGATTGGTGT+TGG + Chr8:77033154-77033173 None:intergenic 50.0%
GTTAGCGTGACGAAGGAGAT+TGG + Chr8:77033160-77033179 None:intergenic 50.0%
TACCGCGTTTACCGTTACTG+GGG - Chr8:77033650-77033669 MsG0880046704.01.T01:CDS 50.0%
TCAGCGAGAGCAACAAGAAG+AGG + Chr8:77033216-77033235 None:intergenic 50.0%
!! CGACGTGAATGCGTTTTTGG+CGG - Chr8:77033557-77033576 MsG0880046704.01.T01:CDS 50.0%
CAGCAATCGGTGACTGGAAG+TGG - Chr8:77033783-77033802 MsG0880046704.01.T01:CDS 55.0%
CTCGCTGATACTGCGTTTCG+CGG - Chr8:77033228-77033247 MsG0880046704.01.T01:CDS 55.0%
GACTGGAAGTGGTTCGCAAC+AGG - Chr8:77033794-77033813 MsG0880046704.01.T01:CDS 55.0%
GCATGCTAGTCCAGCTGGTT+TGG - Chr8:77033722-77033741 MsG0880046704.01.T01:CDS 55.0%
GCTACCGCGTTTACCGTTAC+TGG - Chr8:77033648-77033667 MsG0880046704.01.T01:CDS 55.0%
TGGAAGTGGTTCGCAACAGG+CGG - Chr8:77033797-77033816 MsG0880046704.01.T01:CDS 55.0%
TGGTTCGCAACAGGCGGTTG+TGG - Chr8:77033803-77033822 MsG0880046704.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr8 gene 77032880 77033920 77032880 ID=MsG0880046704.01;Name=MsG0880046704.01
Chr8 mRNA 77032880 77033920 77032880 ID=MsG0880046704.01.T01;Parent=MsG0880046704.01;Name=MsG0880046704.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|346
Chr8 exon 77032880 77033920 77032880 ID=MsG0880046704.01.T01:exon:8761;Parent=MsG0880046704.01.T01
Chr8 CDS 77032880 77033920 77032880 ID=MsG0880046704.01.T01:cds;Parent=MsG0880046704.01.T01
Gene Sequence

>MsG0880046704.01.T01

ATGTCATCAACAACATTGAAACAACAACACTACGCCACAAGTGGCTTAGTAATCGGTTACGCACTCTGTTCAAGCTTATTAGCAATCATAAACAAATACGCTATTACTCAATTCAACTACCCAGGTCTTTTAACTGCGTTACAATATCTCACTTCTTCACTCGGTGTTTACCTTTTAGGTAAATTAGGGTTTCTTCATCACGATCCTTTCACTATTCCAATCGCTAAGAAATTCTTCCCTGCAGCACTCGTTTTCTTCTTAGCGATCTTCACCAACACCAATCTCCTTCGTCACGCTAACGTTGATACGTTCATTGTTTTCAGATCTCTTACCCCTCTTCTTGTTGCTCTCGCTGATACTGCGTTTCGCGGTCAACCTTCTCCGTCTAATTTTACCTTTTTGTCCTTGGTTGTTATTCTTGCTGGTGCTGTTGGTTATGTTGCTACTGATTCCGGTTTTACACTCACGGCTTATTCTTGGGCTTTTGCTTATTTGGTTACCATTACTACTGAGATGGTTTATATTAAGCATATGGTTATGAGTCTTGGATTGAATACTTGGGGTTTTGTGCTTTATAATAATGTATTGTCATTGATGATTGCTCCTGTTTTTTGGTTTTTGACTGGTGAGAATTTTGAGGTTTTTACTGCCTTGAGATCGAGTACTGGGAGTTTGTTCGACGTGAATGCGTTTTTGGCGGTTTCGTTGTCTTGTGTGTTTGGTTTGTTGATTAGTTTCTTTGGATTTGCTGCTAGAAAAGCTGTTTCTGCTACCGCGTTTACCGTTACTGGGGTTGTGAATAAGTTTCTGACTGTGGCAATTAATGTGACGATTTGGGATAAGCATGCTAGTCCAGCTGGTTTGGTTTGTTTGCTTTTTACGATTATTGGGGGTGTTCTTTATCAGCAATCGGTGACTGGAAGTGGTTCGCAACAGGCGGTTGTGGTGACTAAACAACAGTCTGATGTTGAAAGTAGTCTTGTTGGTGATGGTGATTCTGAAGATGAGAGTGAAGGAAAGGGTAAACATGCTTCTCTATGA

Protein sequence

>MsG0880046704.01.T01

MSSTTLKQQHYATSGLVIGYALCSSLLAIINKYAITQFNYPGLLTALQYLTSSLGVYLLGKLGFLHHDPFTIPIAKKFFPAALVFFLAIFTNTNLLRHANVDTFIVFRSLTPLLVALADTAFRGQPSPSNFTFLSLVVILAGAVGYVATDSGFTLTAYSWAFAYLVTITTEMVYIKHMVMSLGLNTWGFVLYNNVLSLMIAPVFWFLTGENFEVFTALRSSTGSLFDVNAFLAVSLSCVFGLLISFFGFAARKAVSATAFTVTGVVNKFLTVAINVTIWDKHASPAGLVCLLFTIIGGVLYQQSVTGSGSQQAVVVTKQQSDVESSLVGDGDSEDESEGKGKHASL*