AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880046751.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046751.01.T01 MTR_4g114000 97.256 328 9 0 1 328 6 333 0.0 661
MsG0880046751.01.T01 MTR_2g103300 46.201 329 154 8 2 320 1 316 1.37e-91 276
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046751.01.T01 AT1G15410 45.872 327 168 5 3 320 1 327 3.36e-97 291
MsG0880046751.01.T01 AT1G15410 45.872 327 168 5 3 320 1 327 3.36e-97 291

Find 67 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGCTAGGTCAATTATAAAT+TGG 0.165914 8:-77653935 MsG0880046751.01.T01:CDS
CGATTCGATGTGATCTATTC+TGG 0.248327 8:+77654472 None:intergenic
CAAAATACTATTGGTGTTAT+AGG 0.255278 8:-77654647 MsG0880046751.01.T01:CDS
CTGGCATCTGATGATTTGTT+AGG 0.271912 8:-77654014 MsG0880046751.01.T01:CDS
AATTGCAATCCATGTTCTTT+TGG 0.314597 8:-77654060 MsG0880046751.01.T01:CDS
GGGAGTGAATGTGGTGATTC+TGG 0.318342 8:-77654033 MsG0880046751.01.T01:CDS
AATCATCCTCAATTTCTGTT+TGG 0.330947 8:-77654839 MsG0880046751.01.T01:CDS
CAAGGAAAGGCTTCAAAGTC+AGG 0.353035 8:-77654189 MsG0880046751.01.T01:CDS
ATCAGGGAAGTACCATTTCC+TGG 0.376067 8:+77654713 None:intergenic
TCTATTCTGGTTCTTGTAGA+AGG 0.402553 8:+77654485 None:intergenic
GGCTAGGTCAATTATAAATT+GGG 0.406221 8:-77653934 MsG0880046751.01.T01:CDS
TATAGATCCTATGGATGCTT+TGG 0.411384 8:-77653955 MsG0880046751.01.T01:CDS
CTTTGTAGTTTGTTATTACA+AGG 0.412464 8:-77654207 MsG0880046751.01.T01:CDS
AAGATATGTGACCTCTCATC+AGG 0.424860 8:+77654696 None:intergenic
GCAATCTGGAGCTCGCGGTC+TGG 0.427237 8:-77654399 MsG0880046751.01.T01:CDS
AGATCACATCGAATCGAATC+AGG 0.427968 8:-77654465 MsG0880046751.01.T01:CDS
CTAAGCCAACAAAATACTAT+TGG 0.433449 8:-77654656 MsG0880046751.01.T01:CDS
ATAAACGTTCTTCAGCAATC+TGG 0.436621 8:-77654413 MsG0880046751.01.T01:CDS
CTCTGCAAAGCATCCGCAGC+AGG 0.440584 8:+77654116 None:intergenic
TAAGAAATGTATAGATCCTA+TGG 0.442345 8:-77653964 MsG0880046751.01.T01:CDS
CTTGTCATCTGTCACATGCT+TGG 0.447830 8:-77654370 MsG0880046751.01.T01:CDS
AGCTGATGATTCAGAATTTG+CGG 0.455725 8:-77654442 MsG0880046751.01.T01:CDS
TGGCATCTGATGATTTGTTA+GGG 0.460265 8:-77654013 MsG0880046751.01.T01:CDS
TCTTGGAAAAGCTAGCTTGT+TGG 0.468035 8:-77654595 MsG0880046751.01.T01:CDS
ACGGTACTTGTGGCATGAAT+CGG 0.469704 8:+77654260 None:intergenic
AGGATGATTCAGCGTCTGTA+TGG 0.476525 8:+77654853 None:intergenic
AACTATGAGGAACCAGGAAA+TGG 0.476902 8:-77654725 MsG0880046751.01.T01:CDS
AGTCTGAGTTCTGTTTCTAA+AGG 0.482149 8:+77654811 None:intergenic
GGATCACTGCAGACAACAAA+AGG 0.483038 8:+77654545 None:intergenic
TAGTTTGTTATTACAAGGAA+AGG 0.496945 8:-77654202 MsG0880046751.01.T01:CDS
GCCACAAGTACCGTGCGGAT+TGG 0.497549 8:-77654251 MsG0880046751.01.T01:CDS
CTTAGCATTCTTGAGTTCCA+TGG 0.499260 8:+77654289 None:intergenic
TTTGGTGAGGGGAGTGAATG+TGG 0.499971 8:-77654042 MsG0880046751.01.T01:CDS
TCCAATCCGCACGGTACTTG+TGG 0.513320 8:+77654250 None:intergenic
CAGTCGCCAACATGAAGAAA+AGG 0.514256 8:+77654317 None:intergenic
TTGCAGAGAAAAGACATAGA+AGG 0.515880 8:-77654101 MsG0880046751.01.T01:CDS
GAAACAACAACAAAGGTACC+TGG 0.538159 8:-77653909 MsG0880046751.01.T01:CDS
TCTATACATTTCTTAAGAAG+AGG 0.540694 8:+77653972 None:intergenic
TGATGAGAGTGGAAACTATG+AGG 0.558216 8:-77654738 MsG0880046751.01.T01:CDS
GCAACACCAAACAGAAATTG+AGG 0.563924 8:+77654833 None:intergenic
CTCCCCTCACCAAAAGAACA+TGG 0.568950 8:+77654051 None:intergenic
GAAAAGACATAGAAGGTGCA+AGG 0.573204 8:-77654094 MsG0880046751.01.T01:CDS
AAGGAAAGGCTTCAAAGTCA+GGG 0.573789 8:-77654188 MsG0880046751.01.T01:CDS
CAGTGATCCTGTGTTAAGCA+AGG 0.576101 8:-77654531 MsG0880046751.01.T01:CDS
GCTTGTTGGAGTTCAAGAAA+CGG 0.592696 8:-77654581 MsG0880046751.01.T01:CDS
AATACTATTGGTGTTATAGG+AGG 0.597817 8:-77654644 MsG0880046751.01.T01:CDS
TGTTGGCGACTGTGTTGCCA+TGG 0.603805 8:-77654306 MsG0880046751.01.T01:CDS
AGCAAGGCGCTTTCATTACG+CGG 0.605632 8:-77654515 MsG0880046751.01.T01:CDS
ATGTGACAGATGACAAGGCA+AGG 0.610214 8:+77654376 None:intergenic
CAAGCATGTGACAGATGACA+AGG 0.621083 8:+77654371 None:intergenic
CTTGTAATAACAAACTACAA+AGG 0.629407 8:+77654208 None:intergenic
CTTCAGCAATCTGGAGCTCG+CGG 0.639447 8:-77654404 MsG0880046751.01.T01:CDS
ATCCTATGGATGCTTTGGCT+AGG 0.640355 8:-77653950 MsG0880046751.01.T01:CDS
AGTGGAAACTATGAGGAACC+AGG 0.643821 8:-77654731 MsG0880046751.01.T01:CDS
AAACAACAACAAAGGTACCT+GGG 0.645689 8:-77653908 MsG0880046751.01.T01:CDS
GACCTAGCCAAAGCATCCAT+AGG 0.646633 8:+77653948 None:intergenic
TCACTGCAGACAACAAAAGG+TGG 0.652139 8:+77654548 None:intergenic
TTAAGAAGAGGATCATTGTG+AGG 0.656825 8:+77653984 None:intergenic
AATGGTACTTCCCTGATGAG+AGG 0.660714 8:-77654707 MsG0880046751.01.T01:CDS
AGATATGTGACCTCTCATCA+GGG 0.661573 8:+77654697 None:intergenic
GAAAGCGCCTTGCTTAACAC+AGG 0.664592 8:+77654524 None:intergenic
TTGGGCAGAAACAACAACAA+AGG 0.665979 8:-77653916 MsG0880046751.01.T01:CDS
GTTGCTAGACAAAGCGACAG+AGG 0.679319 8:-77654153 MsG0880046751.01.T01:CDS
ACATATACTAGTTCCTGCTG+CGG 0.695004 8:-77654129 MsG0880046751.01.T01:CDS
TTGGCACAGAGAAATTTCGA+AGG 0.697984 8:-77654351 MsG0880046751.01.T01:CDS
AGTGAGCAGTCCAATCCGCA+CGG 0.709253 8:+77654241 None:intergenic
TTCATGCCACAAGTACCGTG+CGG 0.796717 8:-77654256 MsG0880046751.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! CTTGTAATAACAAACTACAA+AGG + Chr8:77654557-77654576 None:intergenic 25.0%
! TAAGAAATGTATAGATCCTA+TGG - Chr8:77654798-77654817 MsG0880046751.01.T01:CDS 25.0%
! TCTATACATTTCTTAAGAAG+AGG + Chr8:77654793-77654812 None:intergenic 25.0%
!! TAACACCAATAGTATTTTGT+TGG + Chr8:77654114-77654133 None:intergenic 25.0%
!!! CAAAATACTATTGGTGTTAT+AGG - Chr8:77654115-77654134 MsG0880046751.01.T01:CDS 25.0%
!!! CTTTGTAGTTTGTTATTACA+AGG - Chr8:77654555-77654574 MsG0880046751.01.T01:CDS 25.0%
!!! TAGTTTGTTATTACAAGGAA+AGG - Chr8:77654560-77654579 MsG0880046751.01.T01:CDS 25.0%
AATCATCCTCAATTTCTGTT+TGG - Chr8:77653923-77653942 MsG0880046751.01.T01:CDS 30.0%
CTAAGCCAACAAAATACTAT+TGG - Chr8:77654106-77654125 MsG0880046751.01.T01:CDS 30.0%
GGCTAGGTCAATTATAAATT+GGG - Chr8:77654828-77654847 MsG0880046751.01.T01:CDS 30.0%
TGGCTAGGTCAATTATAAAT+TGG - Chr8:77654827-77654846 MsG0880046751.01.T01:CDS 30.0%
! AATTGCAATCCATGTTCTTT+TGG - Chr8:77654702-77654721 MsG0880046751.01.T01:CDS 30.0%
!! AATACTATTGGTGTTATAGG+AGG - Chr8:77654118-77654137 MsG0880046751.01.T01:CDS 30.0%
!!! TCTATCAACTCTTGTTTTCT+TGG - Chr8:77654150-77654169 MsG0880046751.01.T01:CDS 30.0%
AGCTGATGATTCAGAATTTG+CGG - Chr8:77654320-77654339 MsG0880046751.01.T01:CDS 35.0%
ATAAACGTTCTTCAGCAATC+TGG - Chr8:77654349-77654368 MsG0880046751.01.T01:CDS 35.0%
TATAGATCCTATGGATGCTT+TGG - Chr8:77654807-77654826 MsG0880046751.01.T01:CDS 35.0%
TTAAGAAGAGGATCATTGTG+AGG + Chr8:77654781-77654800 None:intergenic 35.0%
TTGCAGAGAAAAGACATAGA+AGG - Chr8:77654661-77654680 MsG0880046751.01.T01:CDS 35.0%
! AATCCATGTTCTTTTGGTGA+GGG - Chr8:77654708-77654727 MsG0880046751.01.T01:CDS 35.0%
! GTTTTTCTTTCTGATGAGAG+TGG - Chr8:77654013-77654032 MsG0880046751.01.T01:CDS 35.0%
! TCTCTACCTTTTCTTCATGT+TGG - Chr8:77654439-77654458 MsG0880046751.01.T01:CDS 35.0%
!! AAACAACAACAAAGGTACCT+GGG - Chr8:77654854-77654873 MsG0880046751.01.T01:CDS 35.0%
!! AGTCTGAGTTCTGTTTCTAA+AGG + Chr8:77653954-77653973 None:intergenic 35.0%
!! TCTATTCTGGTTCTTGTAGA+AGG + Chr8:77654280-77654299 None:intergenic 35.0%
!! TGGCATCTGATGATTTGTTA+GGG - Chr8:77654749-77654768 MsG0880046751.01.T01:CDS 35.0%
AACTATGAGGAACCAGGAAA+TGG - Chr8:77654037-77654056 MsG0880046751.01.T01:CDS 40.0%
AAGATATGTGACCTCTCATC+AGG + Chr8:77654069-77654088 None:intergenic 40.0%
AAGGAAAGGCTTCAAAGTCA+GGG - Chr8:77654574-77654593 MsG0880046751.01.T01:CDS 40.0%
ACATATACTAGTTCCTGCTG+CGG - Chr8:77654633-77654652 MsG0880046751.01.T01:CDS 40.0%
AGATATGTGACCTCTCATCA+GGG + Chr8:77654068-77654087 None:intergenic 40.0%
AGATCACATCGAATCGAATC+AGG - Chr8:77654297-77654316 MsG0880046751.01.T01:CDS 40.0%
CGATTCGATGTGATCTATTC+TGG + Chr8:77654293-77654312 None:intergenic 40.0%
CTTAGCATTCTTGAGTTCCA+TGG + Chr8:77654476-77654495 None:intergenic 40.0%
GCAACACCAAACAGAAATTG+AGG + Chr8:77653932-77653951 None:intergenic 40.0%
TGATGAGAGTGGAAACTATG+AGG - Chr8:77654024-77654043 MsG0880046751.01.T01:CDS 40.0%
TTGGCACAGAGAAATTTCGA+AGG - Chr8:77654411-77654430 MsG0880046751.01.T01:CDS 40.0%
TTGGGCAGAAACAACAACAA+AGG - Chr8:77654846-77654865 MsG0880046751.01.T01:CDS 40.0%
! ATCCATGTTCTTTTGGTGAG+GGG - Chr8:77654709-77654728 MsG0880046751.01.T01:CDS 40.0%
! CAATCCATGTTCTTTTGGTG+AGG - Chr8:77654707-77654726 MsG0880046751.01.T01:CDS 40.0%
! GAAAAGACATAGAAGGTGCA+AGG - Chr8:77654668-77654687 MsG0880046751.01.T01:CDS 40.0%
! GCTTGTTGGAGTTCAAGAAA+CGG - Chr8:77654181-77654200 MsG0880046751.01.T01:CDS 40.0%
! TCTTGGAAAAGCTAGCTTGT+TGG - Chr8:77654167-77654186 MsG0880046751.01.T01:CDS 40.0%
!! CTGGCATCTGATGATTTGTT+AGG - Chr8:77654748-77654767 MsG0880046751.01.T01:CDS 40.0%
!! GAAACAACAACAAAGGTACC+TGG - Chr8:77654853-77654872 MsG0880046751.01.T01:CDS 40.0%
AATGGTACTTCCCTGATGAG+AGG - Chr8:77654055-77654074 MsG0880046751.01.T01:CDS 45.0%
ACGGTACTTGTGGCATGAAT+CGG + Chr8:77654505-77654524 None:intergenic 45.0%
AGGATGATTCAGCGTCTGTA+TGG + Chr8:77653912-77653931 None:intergenic 45.0%
AGTGGAAACTATGAGGAACC+AGG - Chr8:77654031-77654050 MsG0880046751.01.T01:CDS 45.0%
ATCCTATGGATGCTTTGGCT+AGG - Chr8:77654812-77654831 MsG0880046751.01.T01:CDS 45.0%
ATGTGACAGATGACAAGGCA+AGG + Chr8:77654389-77654408 None:intergenic 45.0%
CAAGCATGTGACAGATGACA+AGG + Chr8:77654394-77654413 None:intergenic 45.0%
CAAGGAAAGGCTTCAAAGTC+AGG - Chr8:77654573-77654592 MsG0880046751.01.T01:CDS 45.0%
CAGTCGCCAACATGAAGAAA+AGG + Chr8:77654448-77654467 None:intergenic 45.0%
CAGTGATCCTGTGTTAAGCA+AGG - Chr8:77654231-77654250 MsG0880046751.01.T01:CDS 45.0%
CTTGTCATCTGTCACATGCT+TGG - Chr8:77654392-77654411 MsG0880046751.01.T01:CDS 45.0%
GGATCACTGCAGACAACAAA+AGG + Chr8:77654220-77654239 None:intergenic 45.0%
TCACTGCAGACAACAAAAGG+TGG + Chr8:77654217-77654236 None:intergenic 45.0%
!! ATCAGGGAAGTACCATTTCC+TGG + Chr8:77654052-77654071 None:intergenic 45.0%
CTCCCCTCACCAAAAGAACA+TGG + Chr8:77654714-77654733 None:intergenic 50.0%
GGGAGTGAATGTGGTGATTC+TGG - Chr8:77654729-77654748 MsG0880046751.01.T01:CDS 50.0%
GTTGCTAGACAAAGCGACAG+AGG - Chr8:77654609-77654628 MsG0880046751.01.T01:CDS 50.0%
TTTGGTGAGGGGAGTGAATG+TGG - Chr8:77654720-77654739 MsG0880046751.01.T01:CDS 50.0%
! AGCAAGGCGCTTTCATTACG+CGG - Chr8:77654247-77654266 MsG0880046751.01.T01:CDS 50.0%
! GACCTAGCCAAAGCATCCAT+AGG + Chr8:77654817-77654836 None:intergenic 50.0%
! TTCATGCCACAAGTACCGTG+CGG - Chr8:77654506-77654525 MsG0880046751.01.T01:CDS 50.0%
!! GAAAGCGCCTTGCTTAACAC+AGG + Chr8:77654241-77654260 None:intergenic 50.0%
AGTGAGCAGTCCAATCCGCA+CGG + Chr8:77654524-77654543 None:intergenic 55.0%
TCCAATCCGCACGGTACTTG+TGG + Chr8:77654515-77654534 None:intergenic 55.0%
TGTTGGCGACTGTGTTGCCA+TGG - Chr8:77654456-77654475 MsG0880046751.01.T01:CDS 55.0%
! CTTCAGCAATCTGGAGCTCG+CGG - Chr8:77654358-77654377 MsG0880046751.01.T01:CDS 55.0%
! CTCTGCAAAGCATCCGCAGC+AGG + Chr8:77654649-77654668 None:intergenic 60.0%
! GCCACAAGTACCGTGCGGAT+TGG - Chr8:77654511-77654530 MsG0880046751.01.T01:CDS 60.0%
! GCAATCTGGAGCTCGCGGTC+TGG - Chr8:77654363-77654382 MsG0880046751.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr8 gene 77653899 77654885 77653899 ID=MsG0880046751.01;Name=MsG0880046751.01
Chr8 mRNA 77653899 77654885 77653899 ID=MsG0880046751.01.T01;Parent=MsG0880046751.01;Name=MsG0880046751.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|328
Chr8 exon 77653899 77654885 77653899 ID=MsG0880046751.01.T01:exon:18474;Parent=MsG0880046751.01.T01
Chr8 CDS 77653899 77654885 77653899 ID=MsG0880046751.01.T01:cds;Parent=MsG0880046751.01.T01
Gene Sequence

>MsG0880046751.01.T01

ATGTTGATGTCCATACAGACGCTGAATCATCCTCAATTTCTGTTTGGTGTTGCCTTTAGAAACAGAACTCAGACTCTATACAAAACAAGATCATCAAATTCAAGTATATCTTCAGTTTTTCTTTCTGATGAGAGTGGAAACTATGAGGAACCAGGAAATGGTACTTCCCTGATGAGAGGTCACATATCTTCTGCATCATGCATCAAGCTAAGCCAACAAAATACTATTGGTGTTATAGGAGGAGTATCAGTTCTATCAACTCTTGTTTTCTTGGAAAAGCTAGCTTGTTGGAGTTCAAGAAACGGTAAAGAATGTCCACCTTTTGTTGTCTGCAGTGATCCTGTGTTAAGCAAGGCGCTTTCATTACGCGGTTCTTTTCCTTCTACAAGAACCAGAATAGATCACATCGAATCGAATCAGGAGCTGATGATTCAGAATTTGCGGCACAAGATAAACGTTCTTCAGCAATCTGGAGCTCGCGGTCTGGCCTTGCCTTGTCATCTGTCACATGCTTGGCACAGAGAAATTTCGAAGGATAGTTCTCTACCTTTTCTTCATGTTGGCGACTGTGTTGCCATGGAACTCAAGAATGCTAAGATGAAGCCGATTCATGCCACAAGTACCGTGCGGATTGGACTGCTCACTACAGATTCATCCTTTGTAGTTTGTTATTACAAGGAAAGGCTTCAAAGTCAGGGTTTTGAAGTAGTGTTGCTAGACAAAGCGACAGAGGAACATATACTAGTTCCTGCTGCGGATGCTTTGCAGAGAAAAGACATAGAAGGTGCAAGGAATTTGCTGAGAATTGCAATCCATGTTCTTTTGGTGAGGGGAGTGAATGTGGTGATTCTGGCATCTGATGATTTGTTAGGGATTCTTCCTCACAATGATCCTCTTCTTAAGAAATGTATAGATCCTATGGATGCTTTGGCTAGGTCAATTATAAATTGGGCAGAAACAACAACAAAGGTACCTGGGAGTTTTTAG
Protein sequence

>MsG0880046751.01.T01

MLMSIQTLNHPQFLFGVAFRNRTQTLYKTRSSNSSISSVFLSDESGNYEEPGNGTSLMRGHISSASCIKLSQQNTIGVIGGVSVLSTLVFLEKLACWSSRNGKECPPFVVCSDPVLSKALSLRGSFPSTRTRIDHIESNQELMIQNLRHKINVLQQSGARGLALPCHLSHAWHREISKDSSLPFLHVGDCVAMELKNAKMKPIHATSTVRIGLLTTDSSFVVCYYKERLQSQGFEVVLLDKATEEHILVPAADALQRKDIEGARNLLRIAIHVLLVRGVNVVILASDDLLGILPHNDPLLKKCIDPMDALARSIINWAETTTKVPGSF*