Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046976.01.T01 | XP_039689652.1 | 93.478 | 138 | 7 | 1 | 1 | 136 | 1 | 138 | 1.05E-84 | 254 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046976.01.T01 | I3SXV9 | 93.478 | 138 | 7 | 1 | 1 | 136 | 1 | 138 | 5.02e-85 | 254 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380014258.01 | MsG0880046976.01 | 0.839679 | 1.371947e-57 | 1.908562e-54 |
| MsG0280010160.01 | MsG0880046976.01 | 0.817938 | 2.543479e-52 | 1.877826e-49 |
| MsG0280010484.01 | MsG0880046976.01 | 0.813194 | 2.896383e-51 | 1.878822e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046976.01.T01 | MTR_4g118490 | 73.188 | 138 | 4 | 2 | 1 | 136 | 1 | 107 | 1.14e-61 | 185 |
| MsG0880046976.01.T01 | MTR_2g020830 | 40.299 | 201 | 52 | 8 | 1 | 145 | 1 | 189 | 2.00e-26 | 98.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046976.01.T01 | AT3G21570 | 34.615 | 156 | 69 | 6 | 1 | 145 | 1 | 134 | 2.33e-19 | 79.0 |
Find 46 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCAAGACCGCAGCAGTTTC+CGG | 0.246506 | 8:+80572791 | None:intergenic |
| TTTAGTCCTGGTCGGGTAAC+CGG | 0.353617 | 8:+80572842 | None:intergenic |
| CTGGTCGGGTAACCGGTTTC+CGG | 0.394516 | 8:+80572849 | None:intergenic |
| ACTCTGCGACGCGAGATCTA+AGG | 0.398943 | 8:-80572562 | MsG0880046976.01.T01:CDS |
| TACGCTGGATCTGCTGCGTT+TGG | 0.432939 | 8:-80572659 | MsG0880046976.01.T01:CDS |
| GGCCGTGAGCTTATATCAAC+CGG | 0.443850 | 8:-80572810 | MsG0880046976.01.T01:CDS |
| CAGGGGTAAGTCGTTGGATT+CGG | 0.444527 | 8:-80572696 | MsG0880046976.01.T01:CDS |
| GCCACAGGCTTCTTTAGTCC+TGG | 0.452358 | 8:+80572830 | None:intergenic |
| AGCGTTGATGATTCAGTCAC+TGG | 0.459423 | 8:-80572761 | MsG0880046976.01.T01:CDS |
| GTGGTTGCTCCGGTGAGAAA+AGG | 0.467581 | 8:+80572910 | None:intergenic |
| AGGCTTCTTTAGTCCTGGTC+GGG | 0.477025 | 8:+80572835 | None:intergenic |
| TGTTTCTTCATCAGAAAAGA+TGG | 0.480616 | 8:+80572605 | None:intergenic |
| TGATATAAGCTCACGGCCAC+AGG | 0.484269 | 8:+80572815 | None:intergenic |
| CAGGCTTCTTTAGTCCTGGT+CGG | 0.491524 | 8:+80572834 | None:intergenic |
| GAGTGATGAAATAGTCTTGA+GGG | 0.495538 | 8:+80572939 | None:intergenic |
| CGGAAACCGGTTACCCGACC+AGG | 0.496212 | 8:-80572848 | MsG0880046976.01.T01:CDS |
| CTCAAGGTATCACCGGAAAC+CGG | 0.498548 | 8:-80572861 | MsG0880046976.01.T01:CDS |
| TTCATCAGAAAAGATGGTAA+AGG | 0.502587 | 8:+80572611 | None:intergenic |
| CAGCGGTTCAGCTGTGGAGA+AGG | 0.504992 | 8:-80572732 | MsG0880046976.01.T01:CDS |
| TCTCCGCAGGGGTAAGTCGT+TGG | 0.512292 | 8:-80572702 | MsG0880046976.01.T01:CDS |
| TGGTTGCTCCGGTGAGAAAA+GGG | 0.516156 | 8:+80572911 | None:intergenic |
| CTCCGGTGAGAAAAGGGAGC+AGG | 0.534291 | 8:+80572917 | None:intergenic |
| AAAGGAAGCGCGCTCGGCGA+CGG | 0.541743 | 8:+80572629 | None:intergenic |
| GATGGTAAAGGAAGCGCGCT+CGG | 0.544971 | 8:+80572623 | None:intergenic |
| AGAAGGTCACGATTCTCCGC+AGG | 0.545671 | 8:-80572715 | MsG0880046976.01.T01:CDS |
| CACGGTTGTTGTAGCCGTAG+TGG | 0.565228 | 8:+80572891 | None:intergenic |
| CCGGTGATACCTTGAGGTGA+CGG | 0.577744 | 8:+80572868 | None:intergenic |
| GAAGAGCAGCGGTTCAGCTG+TGG | 0.585784 | 8:-80572738 | MsG0880046976.01.T01:CDS |
| GTGAAGATCGACACATACGC+TGG | 0.586303 | 8:-80572674 | MsG0880046976.01.T01:CDS |
| TGTAGCCGTAGTGGTTGCTC+CGG | 0.593393 | 8:+80572900 | None:intergenic |
| CCGTCACCTCAAGGTATCAC+CGG | 0.597704 | 8:-80572868 | MsG0880046976.01.T01:CDS |
| GAAGGTCACGATTCTCCGCA+GGG | 0.598156 | 8:-80572714 | MsG0880046976.01.T01:CDS |
| CGGTTTCCGGTGATACCTTG+AGG | 0.601868 | 8:+80572862 | None:intergenic |
| GTATAAAACTTGAGTTCCCA+TGG | 0.609224 | 8:+80572961 | None:intergenic |
| ACAACCGTGCCGTCACCTCA+AGG | 0.615765 | 8:-80572877 | MsG0880046976.01.T01:CDS |
| GAAGAAACAGTTGGCTGTGT+CGG | 0.621267 | 8:-80572591 | MsG0880046976.01.T01:CDS |
| TTCCGGTTGATATAAGCTCA+CGG | 0.623767 | 8:+80572808 | None:intergenic |
| GCGTTGATGATTCAGTCACT+GGG | 0.645202 | 8:-80572760 | MsG0880046976.01.T01:CDS |
| ATATCAACCGGAAACTGCTG+CGG | 0.646214 | 8:-80572798 | MsG0880046976.01.T01:CDS |
| TCTCACCGGAGCAACCACTA+CGG | 0.649996 | 8:-80572905 | MsG0880046976.01.T01:CDS |
| TCAGTCACTGGGAAGAGCAG+CGG | 0.654529 | 8:-80572749 | MsG0880046976.01.T01:CDS |
| GATACCTTGAGGTGACGGCA+CGG | 0.673623 | 8:+80572873 | None:intergenic |
| ACCAGGACTAAAGAAGCCTG+TGG | 0.675004 | 8:-80572831 | MsG0880046976.01.T01:CDS |
| GGAGTGATGAAATAGTCTTG+AGG | 0.692580 | 8:+80572938 | None:intergenic |
| AAGGTCACGATTCTCCGCAG+GGG | 0.732255 | 8:-80572713 | MsG0880046976.01.T01:CDS |
| AATCCAACGACTTACCCCTG+CGG | 0.825351 | 8:+80572699 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TGTTTCTTCATCAGAAAAGA+TGG | + | Chr8:80572898-80572917 | None:intergenic | 30.0% | |
| TTCATCAGAAAAGATGGTAA+AGG | + | Chr8:80572892-80572911 | None:intergenic | 30.0% | |
| ! | TTTTCTGATGAAGAAACAGT+TGG | - | Chr8:80572900-80572919 | MsG0880046976.01.T01:CDS | 30.0% |
| ! | GAGTGATGAAATAGTCTTGA+GGG | + | Chr8:80572564-80572583 | None:intergenic | 35.0% |
| ! | GGAGTGATGAAATAGTCTTG+AGG | + | Chr8:80572565-80572584 | None:intergenic | 40.0% |
| ! | TTCCGGTTGATATAAGCTCA+CGG | + | Chr8:80572695-80572714 | None:intergenic | 40.0% |
| ATATCAACCGGAAACTGCTG+CGG | - | Chr8:80572702-80572721 | MsG0880046976.01.T01:CDS | 45.0% | |
| GAAGAAACAGTTGGCTGTGT+CGG | - | Chr8:80572909-80572928 | MsG0880046976.01.T01:CDS | 45.0% | |
| !! | AGCGTTGATGATTCAGTCAC+TGG | - | Chr8:80572739-80572758 | MsG0880046976.01.T01:CDS | 45.0% |
| !! | GCGTTGATGATTCAGTCACT+GGG | - | Chr8:80572740-80572759 | MsG0880046976.01.T01:CDS | 45.0% |
| AATCCAACGACTTACCCCTG+CGG | + | Chr8:80572804-80572823 | None:intergenic | 50.0% | |
| ACCAGGACTAAAGAAGCCTG+TGG | - | Chr8:80572669-80572688 | MsG0880046976.01.T01:CDS | 50.0% | |
| AGGCTTCTTTAGTCCTGGTC+GGG | + | Chr8:80572668-80572687 | None:intergenic | 50.0% | |
| CAGGCTTCTTTAGTCCTGGT+CGG | + | Chr8:80572669-80572688 | None:intergenic | 50.0% | |
| CAGGGGTAAGTCGTTGGATT+CGG | - | Chr8:80572804-80572823 | MsG0880046976.01.T01:CDS | 50.0% | |
| GGCCGTGAGCTTATATCAAC+CGG | - | Chr8:80572690-80572709 | MsG0880046976.01.T01:CDS | 50.0% | |
| GTGAAGATCGACACATACGC+TGG | - | Chr8:80572826-80572845 | MsG0880046976.01.T01:CDS | 50.0% | |
| TGATATAAGCTCACGGCCAC+AGG | + | Chr8:80572688-80572707 | None:intergenic | 50.0% | |
| TGGTTGCTCCGGTGAGAAAA+GGG | + | Chr8:80572592-80572611 | None:intergenic | 50.0% | |
| TTCAAGACCGCAGCAGTTTC+CGG | + | Chr8:80572712-80572731 | None:intergenic | 50.0% | |
| TTTAGTCCTGGTCGGGTAAC+CGG | + | Chr8:80572661-80572680 | None:intergenic | 50.0% | |
| ! | CTCAAGGTATCACCGGAAAC+CGG | - | Chr8:80572639-80572658 | MsG0880046976.01.T01:CDS | 50.0% |
| AAGGTCACGATTCTCCGCAG+GGG | - | Chr8:80572787-80572806 | MsG0880046976.01.T01:CDS | 55.0% | |
| ACTCTGCGACGCGAGATCTA+AGG | - | Chr8:80572938-80572957 | MsG0880046976.01.T01:CDS | 55.0% | |
| AGAAGGTCACGATTCTCCGC+AGG | - | Chr8:80572785-80572804 | MsG0880046976.01.T01:CDS | 55.0% | |
| CACGGTTGTTGTAGCCGTAG+TGG | + | Chr8:80572612-80572631 | None:intergenic | 55.0% | |
| CCGGTGATACCTTGAGGTGA+CGG | + | Chr8:80572635-80572654 | None:intergenic | 55.0% | |
| CGGTTTCCGGTGATACCTTG+AGG | + | Chr8:80572641-80572660 | None:intergenic | 55.0% | |
| GAAGGTCACGATTCTCCGCA+GGG | - | Chr8:80572786-80572805 | MsG0880046976.01.T01:CDS | 55.0% | |
| GCCACAGGCTTCTTTAGTCC+TGG | + | Chr8:80572673-80572692 | None:intergenic | 55.0% | |
| GTGGTTGCTCCGGTGAGAAA+AGG | + | Chr8:80572593-80572612 | None:intergenic | 55.0% | |
| TACGCTGGATCTGCTGCGTT+TGG | - | Chr8:80572841-80572860 | MsG0880046976.01.T01:CDS | 55.0% | |
| TCAGTCACTGGGAAGAGCAG+CGG | - | Chr8:80572751-80572770 | MsG0880046976.01.T01:CDS | 55.0% | |
| TCTCACCGGAGCAACCACTA+CGG | - | Chr8:80572595-80572614 | MsG0880046976.01.T01:CDS | 55.0% | |
| TGTAGCCGTAGTGGTTGCTC+CGG | + | Chr8:80572603-80572622 | None:intergenic | 55.0% | |
| ! | CCGTCACCTCAAGGTATCAC+CGG | - | Chr8:80572632-80572651 | MsG0880046976.01.T01:CDS | 55.0% |
| ! | CTCCTGCTCCCTTTTCTCAC+CGG | - | Chr8:80572581-80572600 | MsG0880046976.01.T01:CDS | 55.0% |
| ! | GATACCTTGAGGTGACGGCA+CGG | + | Chr8:80572630-80572649 | None:intergenic | 55.0% |
| ! | GATGGTAAAGGAAGCGCGCT+CGG | + | Chr8:80572880-80572899 | None:intergenic | 55.0% |
| ACAACCGTGCCGTCACCTCA+AGG | - | Chr8:80572623-80572642 | MsG0880046976.01.T01:CDS | 60.0% | |
| CAGCGGTTCAGCTGTGGAGA+AGG | - | Chr8:80572768-80572787 | MsG0880046976.01.T01:CDS | 60.0% | |
| CTCCGGTGAGAAAAGGGAGC+AGG | + | Chr8:80572586-80572605 | None:intergenic | 60.0% | |
| GAAGAGCAGCGGTTCAGCTG+TGG | - | Chr8:80572762-80572781 | MsG0880046976.01.T01:CDS | 60.0% | |
| TCTCCGCAGGGGTAAGTCGT+TGG | - | Chr8:80572798-80572817 | MsG0880046976.01.T01:CDS | 60.0% | |
| ! | CTGGTCGGGTAACCGGTTTC+CGG | + | Chr8:80572654-80572673 | None:intergenic | 60.0% |
| CGGAAACCGGTTACCCGACC+AGG | - | Chr8:80572652-80572671 | MsG0880046976.01.T01:CDS | 65.0% | |
| ! | AAAGGAAGCGCGCTCGGCGA+CGG | + | Chr8:80572874-80572893 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 80572541 | 80572981 | 80572541 | ID=MsG0880046976.01;Name=MsG0880046976.01 |
| Chr8 | mRNA | 80572541 | 80572981 | 80572541 | ID=MsG0880046976.01.T01;Parent=MsG0880046976.01;Name=MsG0880046976.01.T01;_AED=0.42;_eAED=0.42;_QI=0|-1|0|1|-1|1|1|0|146 |
| Chr8 | exon | 80572541 | 80572981 | 80572541 | ID=MsG0880046976.01.T01:exon:34666;Parent=MsG0880046976.01.T01 |
| Chr8 | CDS | 80572541 | 80572981 | 80572541 | ID=MsG0880046976.01.T01:cds;Parent=MsG0880046976.01.T01 |
| Gene Sequence |
| Protein sequence |
