AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880047114.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047114.01.T01 MTR_4g121560 96.594 411 14 0 2 412 56 466 0.0 840
MsG0880047114.01.T01 MTR_7g093450 57.265 351 146 3 55 403 243 591 1.54e-157 457
MsG0880047114.01.T01 MTR_0136s0010 56.160 349 149 3 55 401 165 511 2.81e-154 446
MsG0880047114.01.T01 MTR_5g033800 52.815 373 165 4 30 400 121 484 3.54e-149 432
MsG0880047114.01.T01 MTR_5g059400 51.042 384 181 5 22 400 135 516 1.20e-147 429
MsG0880047114.01.T01 MTR_1g116130 51.042 384 181 5 22 400 135 516 1.20e-147 429
MsG0880047114.01.T01 MTR_8g069790 55.685 343 147 3 60 400 178 517 1.20e-146 427
MsG0880047114.01.T01 MTR_4g126950 45.407 381 200 4 28 405 77 452 5.11e-133 390
MsG0880047114.01.T01 MTR_7g109550 48.387 341 159 5 60 396 137 464 2.12e-118 353
MsG0880047114.01.T01 MTR_5g033800 50.929 269 123 3 30 298 121 380 3.09e-100 303
MsG0880047114.01.T01 MTR_1g058380 36.430 409 244 5 8 409 5 404 1.73e-96 295
MsG0880047114.01.T01 MTR_3g464570 39.943 353 186 6 58 400 41 377 2.82e-96 293
MsG0880047114.01.T01 MTR_2g022560 39.489 352 202 4 59 404 96 442 2.24e-95 293
MsG0880047114.01.T01 MTR_7g094750 40.625 352 201 5 54 400 85 433 5.17e-95 292
MsG0880047114.01.T01 MTR_3g090120 41.449 345 187 4 59 400 128 460 4.50e-93 288
MsG0880047114.01.T01 MTR_5g071350 40.390 359 184 7 58 400 56 400 3.84e-90 278
MsG0880047114.01.T01 MTR_2g015720 38.021 384 203 10 17 399 9 358 5.04e-90 276
MsG0880047114.01.T01 MTR_4g087730 39.071 366 195 8 39 401 24 364 2.70e-89 275
MsG0880047114.01.T01 MTR_2g073510 41.399 343 176 10 60 399 33 353 1.47e-87 270
MsG0880047114.01.T01 MTR_4g088030 39.489 352 183 9 54 401 27 352 3.21e-85 264
MsG0880047114.01.T01 MTR_3g049330 39.769 347 181 8 58 400 29 351 6.28e-85 263
MsG0880047114.01.T01 MTR_5g034810 40.922 347 173 10 59 400 34 353 1.17e-84 263
MsG0880047114.01.T01 MTR_8g093530 37.028 397 227 8 12 400 36 417 2.30e-84 265
MsG0880047114.01.T01 MTR_1g050448 38.304 342 190 7 60 399 53 375 7.78e-84 261
MsG0880047114.01.T01 MTR_4g014440 37.569 362 202 9 54 400 56 408 8.18e-83 259
MsG0880047114.01.T01 MTR_4g016940 36.798 356 203 8 56 399 82 427 1.21e-82 259
MsG0880047114.01.T01 MTR_4g079700 34.826 402 220 11 1 399 1 363 9.96e-82 255
MsG0880047114.01.T01 MTR_2g073500 40.462 346 173 12 60 400 29 346 1.01e-81 255
MsG0880047114.01.T01 MTR_1g111010 40.290 345 168 11 60 400 32 342 2.80e-81 254
MsG0880047114.01.T01 MTR_1g112210 37.228 368 206 9 37 399 35 382 6.20e-81 254
MsG0880047114.01.T01 MTR_2g091175 39.942 343 179 9 60 400 50 367 1.41e-80 253
MsG0880047114.01.T01 MTR_1g112210 36.512 367 197 8 37 399 35 369 9.27e-79 248
MsG0880047114.01.T01 MTR_4g014430 37.500 368 203 11 46 399 92 446 9.80e-79 250
MsG0880047114.01.T01 MTR_3g021060 36.919 344 187 9 60 399 46 363 1.43e-78 247
MsG0880047114.01.T01 MTR_7g070060 38.082 365 196 10 53 399 57 409 3.44e-78 248
MsG0880047114.01.T01 MTR_3g035830 35.860 343 194 7 59 399 30 348 5.94e-78 245
MsG0880047114.01.T01 MTR_3g021090 36.283 339 190 7 59 395 30 344 3.17e-75 240
MsG0880047114.01.T01 MTR_8g463790 33.978 362 208 7 60 406 71 416 4.19e-74 237
MsG0880047114.01.T01 MTR_3g070900 35.933 359 198 8 60 400 106 450 1.78e-73 237
MsG0880047114.01.T01 MTR_3g071650 33.500 400 240 10 17 400 42 431 4.87e-73 235
MsG0880047114.01.T01 MTR_5g082880 35.150 367 193 10 60 400 165 512 3.75e-72 235
MsG0880047114.01.T01 MTR_3g464570 38.811 286 150 5 125 400 3 273 1.54e-69 221
MsG0880047114.01.T01 MTR_5g083260 34.278 353 210 7 60 400 83 425 7.86e-68 221
MsG0880047114.01.T01 MTR_7g068920 31.844 358 218 6 56 399 76 421 4.75e-66 216
MsG0880047114.01.T01 MTR_7g094750 38.367 245 150 1 54 298 85 328 3.31e-65 211
MsG0880047114.01.T01 MTR_7g070060 36.164 318 172 11 101 399 2 307 7.93e-55 184
MsG0880047114.01.T01 MTR_7g070060 36.290 248 145 4 53 296 57 295 4.79e-50 171
MsG0880047114.01.T01 MTR_1g076690 29.602 402 248 11 18 399 184 570 5.12e-49 175
MsG0880047114.01.T01 MTR_7g029590 78.641 103 7 2 215 306 1 99 2.68e-46 159
MsG0880047114.01.T01 MTR_6g061710 29.348 368 228 10 52 399 159 514 4.95e-46 166
MsG0880047114.01.T01 MTR_4g092830 28.103 427 247 15 1 401 1 393 7.02e-46 164
MsG0880047114.01.T01 MTR_6g016875 30.024 413 243 12 10 401 23 410 2.46e-44 159
MsG0880047114.01.T01 MTR_6g016880 30.155 388 238 11 27 399 50 419 1.33e-43 157
MsG0880047114.01.T01 MTR_5g035150 29.917 361 220 13 59 399 84 431 7.66e-43 155
MsG0880047114.01.T01 MTR_5g035080 29.455 404 232 13 26 399 40 420 9.15e-43 155
MsG0880047114.01.T01 MTR_5g035070 30.874 366 216 14 56 399 83 433 3.33e-42 154
MsG0880047114.01.T01 MTR_8g093870 31.405 363 205 15 59 399 132 472 4.31e-42 154
MsG0880047114.01.T01 MTR_2g016060 29.006 362 226 13 58 400 52 401 5.09e-42 153
MsG0880047114.01.T01 MTR_8g027900 30.280 393 225 14 35 402 190 558 5.59e-42 155
MsG0880047114.01.T01 MTR_8g099300 26.667 420 274 12 1 400 15 420 8.09e-42 152
MsG0880047114.01.T01 MTR_5g035090 28.415 366 219 12 58 399 81 427 4.79e-41 150
MsG0880047114.01.T01 MTR_3g109840 29.280 403 229 16 21 399 45 415 8.91e-41 150
MsG0880047114.01.T01 MTR_6g016240 30.997 371 202 15 59 399 70 416 9.30e-41 150
MsG0880047114.01.T01 MTR_7g033230 29.917 361 219 11 59 399 263 609 5.35e-40 150
MsG0880047114.01.T01 MTR_7g019120 28.378 370 229 10 49 399 68 420 5.59e-40 148
MsG0880047114.01.T01 MTR_6g007777 28.000 375 224 11 49 399 105 457 6.01e-40 149
MsG0880047114.01.T01 MTR_4g125060 28.297 364 229 14 52 399 124 471 7.03e-40 148
MsG0880047114.01.T01 MTR_4g125060 28.297 364 229 14 52 399 192 539 1.30e-39 149
MsG0880047114.01.T01 MTR_7g033230 29.917 361 219 11 59 399 333 679 1.35e-39 150
MsG0880047114.01.T01 MTR_4g125060 28.297 364 229 14 52 399 163 510 1.63e-39 148
MsG0880047114.01.T01 MTR_8g104970 29.268 369 209 15 60 399 120 465 2.32e-38 144
MsG0880047114.01.T01 MTR_2g022580 41.600 125 73 0 121 245 2 126 1.56e-31 117
MsG0880047114.01.T01 MTR_5g069320 29.139 302 136 9 107 392 147 386 9.03e-31 122
MsG0880047114.01.T01 MTR_7g019140 40.594 101 59 1 49 149 68 167 7.26e-23 95.1
MsG0880047114.01.T01 MTR_3g021150 39.815 108 60 2 257 362 2 106 3.19e-22 91.7
MsG0880047114.01.T01 MTR_5g035140 29.752 242 146 9 178 399 28 265 4.43e-20 89.7
MsG0880047114.01.T01 MTR_5g035120 30.921 152 96 2 11 154 18 168 1.74e-19 85.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047114.01.T01 AT5G49340 61.804 377 134 6 33 403 73 445 1.06e-176 501
MsG0880047114.01.T01 AT5G06700 54.857 350 154 3 54 401 249 596 3.43e-154 449
MsG0880047114.01.T01 AT3G12060 52.128 376 176 3 28 401 164 537 1.19e-152 443
MsG0880047114.01.T01 AT1G60790 54.593 381 159 6 26 402 165 535 5.69e-152 441
MsG0880047114.01.T01 AT5G20590 47.714 350 176 3 55 401 136 481 6.51e-133 390
MsG0880047114.01.T01 AT3G62390 44.536 366 196 5 36 400 113 472 2.91e-122 363
MsG0880047114.01.T01 AT5G19160 41.690 355 189 5 58 400 97 445 5.79e-103 313
MsG0880047114.01.T01 AT3G06080 42.120 349 193 4 58 400 101 446 1.15e-101 310
MsG0880047114.01.T01 AT3G11570 42.424 330 183 2 74 400 95 420 1.21e-96 296
MsG0880047114.01.T01 AT5G06230 40.496 363 203 4 47 400 7 365 3.05e-93 285
MsG0880047114.01.T01 AT5G06230 40.496 363 203 4 47 400 48 406 1.08e-92 285
MsG0880047114.01.T01 AT1G78710 40.056 357 185 7 48 400 26 357 3.14e-92 282
MsG0880047114.01.T01 AT1G29050 39.481 347 182 6 58 401 59 380 1.14e-91 281
MsG0880047114.01.T01 AT2G34070 37.197 371 208 6 31 399 36 383 5.99e-89 275
MsG0880047114.01.T01 AT5G58600 39.377 353 189 10 58 400 64 401 1.65e-87 271
MsG0880047114.01.T01 AT1G48880 36.010 386 228 5 14 395 65 435 5.99e-87 271
MsG0880047114.01.T01 AT2G30900 42.029 345 175 11 60 400 43 366 1.47e-86 268
MsG0880047114.01.T01 AT2G31110 39.359 343 180 8 60 399 45 362 3.66e-85 264
MsG0880047114.01.T01 AT5G20590 45.902 244 131 1 55 298 136 378 4.65e-85 265
MsG0880047114.01.T01 AT2G42570 37.228 368 205 9 36 400 21 365 1.14e-83 260
MsG0880047114.01.T01 AT3G62390 42.545 275 154 3 36 309 113 384 2.86e-82 258
MsG0880047114.01.T01 AT3G14850 36.705 346 192 8 59 400 33 355 2.14e-81 254
MsG0880047114.01.T01 AT5G01620 34.360 422 239 13 2 399 42 449 2.25e-81 257
MsG0880047114.01.T01 AT5G01620 34.360 422 239 13 2 399 42 449 2.25e-81 257
MsG0880047114.01.T01 AT2G30010 37.293 362 190 11 58 400 54 397 2.58e-80 253
MsG0880047114.01.T01 AT2G30010 37.293 362 190 11 58 400 97 440 4.18e-80 254
MsG0880047114.01.T01 AT5G01620 37.222 360 198 12 54 399 112 457 1.87e-79 253
MsG0880047114.01.T01 AT1G73140 35.246 366 217 8 50 406 58 412 9.74e-78 247
MsG0880047114.01.T01 AT5G01360 36.134 357 205 8 56 399 32 378 3.18e-77 244
MsG0880047114.01.T01 AT5G01360 36.134 357 205 8 56 399 88 434 1.67e-76 244
MsG0880047114.01.T01 AT5G19160 42.324 241 138 1 58 298 97 336 1.75e-73 234
MsG0880047114.01.T01 AT3G54260 35.159 347 197 9 60 400 51 375 2.66e-73 234
MsG0880047114.01.T01 AT3G55990 32.668 401 222 11 28 400 104 484 5.91e-73 236
MsG0880047114.01.T01 AT2G40150 34.146 369 197 11 60 400 71 421 9.63e-73 234
MsG0880047114.01.T01 AT2G38320 33.424 368 217 8 50 400 49 405 3.97e-72 232
MsG0880047114.01.T01 AT3G06080 42.739 241 137 1 58 298 101 340 3.49e-71 228
MsG0880047114.01.T01 AT2G40160 34.218 377 199 11 52 400 69 424 1.42e-70 229
MsG0880047114.01.T01 AT2G40160 34.605 367 201 11 52 395 69 419 2.29e-70 228
MsG0880047114.01.T01 AT3G11030 34.463 354 209 7 60 400 106 449 3.93e-68 223
MsG0880047114.01.T01 AT2G40320 33.616 354 213 8 59 400 80 423 5.84e-67 219
MsG0880047114.01.T01 AT1G78710 34.551 356 153 9 48 400 19 297 2.04e-64 209
MsG0880047114.01.T01 AT2G38320 34.030 335 189 9 85 400 1 322 6.67e-63 206
MsG0880047114.01.T01 AT5G06230 37.931 261 156 2 47 301 48 308 1.07e-58 194
MsG0880047114.01.T01 AT5G58600 39.095 243 131 5 58 298 64 291 2.82e-58 192
MsG0880047114.01.T01 AT2G34070 35.424 271 151 5 131 399 1 249 5.94e-58 190
MsG0880047114.01.T01 AT2G30900 39.914 233 125 6 60 291 43 261 1.01e-53 180
MsG0880047114.01.T01 AT3G14850 35.401 274 151 7 131 400 1 252 1.56e-53 179
MsG0880047114.01.T01 AT2G31110 38.496 226 119 6 177 399 6 214 2.68e-49 166
MsG0880047114.01.T01 AT2G31110 37.931 232 125 6 60 289 45 259 5.09e-49 167
MsG0880047114.01.T01 AT1G01430 30.334 389 233 15 36 400 74 448 6.00e-48 170
MsG0880047114.01.T01 AT4G25360 29.397 398 226 15 37 399 147 524 1.37e-46 168
MsG0880047114.01.T01 AT4G25360 29.397 398 226 15 37 399 147 524 1.37e-46 168
MsG0880047114.01.T01 AT2G14530 29.428 367 214 11 58 399 60 406 3.23e-46 164
MsG0880047114.01.T01 AT5G64470 27.701 361 227 14 60 400 55 401 1.79e-45 162
MsG0880047114.01.T01 AT5G64470 27.324 355 230 13 60 400 55 395 2.57e-45 161
MsG0880047114.01.T01 AT1G70230 27.619 420 242 14 10 399 24 411 7.03e-45 160
MsG0880047114.01.T01 AT4G01080 30.328 366 209 12 58 400 89 431 5.02e-44 159
MsG0880047114.01.T01 AT4G23790 29.056 413 256 16 12 400 29 428 6.03e-44 159
MsG0880047114.01.T01 AT5G20680 29.049 389 242 13 28 399 86 457 6.61e-44 159
MsG0880047114.01.T01 AT5G51640 27.136 398 239 11 30 399 114 488 8.13e-44 160
MsG0880047114.01.T01 AT2G40160 31.641 256 154 6 52 298 69 312 1.26e-43 155
MsG0880047114.01.T01 AT5G20680 29.049 389 242 13 28 399 162 533 1.36e-43 159
MsG0880047114.01.T01 AT5G20680 29.049 389 242 13 28 399 180 551 1.63e-43 160
MsG0880047114.01.T01 AT5G20680 29.049 389 242 13 28 399 180 551 1.63e-43 160
MsG0880047114.01.T01 AT5G20680 29.049 389 242 13 28 399 180 551 1.63e-43 160
MsG0880047114.01.T01 AT5G20680 29.049 389 242 13 28 399 180 551 1.63e-43 160
MsG0880047114.01.T01 AT5G64020 29.155 367 218 16 60 399 57 408 8.84e-43 155
MsG0880047114.01.T01 AT5G15890 28.535 396 229 12 26 399 159 522 2.40e-41 153
MsG0880047114.01.T01 AT3G02440 32.402 358 208 12 60 399 127 468 3.71e-41 152
MsG0880047114.01.T01 AT3G02440 32.402 358 208 12 60 399 135 476 3.92e-41 152
MsG0880047114.01.T01 AT5G15900 30.028 363 213 12 59 399 66 409 7.13e-40 147
MsG0880047114.01.T01 AT5G15900 30.081 369 205 12 59 399 85 428 1.12e-39 147
MsG0880047114.01.T01 AT4G11090 26.429 420 264 15 12 404 29 430 6.96e-39 145
MsG0880047114.01.T01 AT2G37720 28.261 368 216 14 56 399 139 482 8.43e-37 140
MsG0880047114.01.T01 AT3G28150 28.362 409 246 16 11 399 29 410 1.31e-36 138
MsG0880047114.01.T01 AT5G64470 27.203 261 167 10 60 308 55 304 3.29e-31 121
MsG0880047114.01.T01 AT5G64470 27.203 261 167 10 60 308 55 304 4.48e-31 121

Find 71 sgRNAs with CRISPR-Local

Find 107 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCAGACTCTATGAAGTTTC+TGG 0.256981 8:+82128437 MsG0880047114.01.T01:CDS
TGCAGCCACTGGTGCCTTCC+TGG 0.265034 8:+82129385 MsG0880047114.01.T01:CDS
TTTCTGGACGCCGTGAATTC+AGG 0.281214 8:+82128453 MsG0880047114.01.T01:CDS
TTGCAGTAGAAACTACTTTC+AGG 0.309018 8:+82128887 MsG0880047114.01.T01:intron
GGCACCAGTGGCTGCAATCT+TGG 0.321542 8:-82129378 None:intergenic
AGTTCATGTGATATCTTTGA+TGG 0.322128 8:+82128024 MsG0880047114.01.T01:CDS
GTGATATCTTTGATGGCTCT+TGG 0.335472 8:+82128031 MsG0880047114.01.T01:CDS
CAGGAACATGTGGCAATCTT+TGG 0.349555 8:+82128382 MsG0880047114.01.T01:CDS
TCCATTCTTGTACAAGAAAC+GGG 0.351149 8:-82128643 None:intergenic
CCCCGTTTCTTGTACAAGAA+TGG 0.370184 8:+82128642 MsG0880047114.01.T01:CDS
CGGGGATTTCACAAAGTCTA+TGG 0.373882 8:-82128624 None:intergenic
CGCCGTGAATTCAGGATTCA+AGG 0.374839 8:+82128461 MsG0880047114.01.T01:CDS
AAGATAGCTTTCATTTGTAA+TGG 0.380265 8:-82129191 None:intergenic
TTCCTGGAATTCCAGACTCT+TGG 0.388151 8:+82129401 MsG0880047114.01.T01:CDS
GGTAAGAGAGTGGTGTTTGT+AGG 0.388845 8:+82128347 MsG0880047114.01.T01:CDS
AGATAGCTTTCATTTGTAAT+GGG 0.396515 8:-82129190 None:intergenic
CACGAACCATGTTGATAAAC+AGG 0.397803 8:-82128075 None:intergenic
TTCCTCAAAGAGATACTCTT+AGG 0.399557 8:+82128687 MsG0880047114.01.T01:CDS
TTCCAAGAGTCTGGAATTCC+AGG 0.401152 8:-82129403 None:intergenic
TTCCATTCTTGTACAAGAAA+CGG 0.405771 8:-82128644 None:intergenic
ATATATACATATAGGTTTGA+TGG 0.410273 8:+82128299 MsG0880047114.01.T01:intron
AGGAGTTCATTCCAAGAGTC+TGG 0.418122 8:-82129412 None:intergenic
TGATAGCAAAGGCCGTAAAA+TGG 0.424469 8:+82129354 MsG0880047114.01.T01:CDS
AACCTTGAATCCTGAATTCA+CGG 0.434590 8:-82128463 None:intergenic
CCTGGTTCTCTATAAATTGA+AGG 0.434968 8:-82129325 None:intergenic
ATGACAGACTACAGAAAAGA+TGG 0.452500 8:+82129298 MsG0880047114.01.T01:CDS
TTGTAGGAGACTCTTTAAAC+AGG 0.459088 8:+82128363 MsG0880047114.01.T01:CDS
CCTTCAATTTATAGAGAACC+AGG 0.470367 8:+82129325 MsG0880047114.01.T01:CDS
CTATAAGTAGAGTGGAATAG+AGG 0.470717 8:-82129432 None:intergenic
TATATACATATAGGTTTGAT+GGG 0.489213 8:+82128300 MsG0880047114.01.T01:intron
ACTACACGTTTGAAAGAAAG+TGG 0.489898 8:-82127883 None:intergenic
AGCCTAAGAGTATCTCTTTG+AGG 0.490581 8:-82128689 None:intergenic
GCAGCATATCCATGGACAAT+GGG 0.492002 8:+82129214 MsG0880047114.01.T01:CDS
CACTGAAGACATGGGCTAAG+TGG 0.492585 8:+82128961 MsG0880047114.01.T01:CDS
CATGAACCTGTTTATCAACA+TGG 0.495783 8:+82128069 MsG0880047114.01.T01:CDS
CAGTTGATAGCACACGTACA+AGG 0.501169 8:+82128994 MsG0880047114.01.T01:CDS
TTGAGTTTCTTAAATATCGT+TGG 0.502006 8:+82128148 MsG0880047114.01.T01:CDS
GAAAGTGGAAAACGAAGAGA+AGG 0.514714 8:-82127868 None:intergenic
TCGACAATTCCCATTGTCCA+TGG 0.522167 8:-82129223 None:intergenic
TTAAAAGTATCATCAAGAAA+AGG 0.530161 8:-82128099 None:intergenic
AACTCTGGTGGTAAATGTGA+TGG 0.532037 8:+82129157 MsG0880047114.01.T01:CDS
TGGTCCAAGATTGCAGCCAC+TGG 0.541196 8:+82129374 MsG0880047114.01.T01:CDS
TGTTTGAGCTATAAGTAGAG+TGG 0.548115 8:-82129440 None:intergenic
GCTATCTTGCAGCATATCCA+TGG 0.549123 8:+82129206 MsG0880047114.01.T01:CDS
CACATGGTTGTCAAATTCCA+AGG 0.549591 8:+82128175 MsG0880047114.01.T01:CDS
ACGAAGAGAAGGAATGGTAC+TGG 0.551618 8:-82127857 None:intergenic
CCTTTGCTATCACTGAAACC+TGG 0.558451 8:-82129343 None:intergenic
ACTCTGGTGGTAAATGTGAT+GGG 0.558567 8:+82129158 MsG0880047114.01.T01:CDS
AGCATCATAATACTGAGACT+TGG 0.567908 8:-82128729 None:intergenic
ACTGAAGACATGGGCTAAGT+GGG 0.576942 8:+82128962 MsG0880047114.01.T01:CDS
TGGAAAACGAAGAGAAGGAA+TGG 0.584656 8:-82127863 None:intergenic
GAAACTATGTAAGAAGTGAA+TGG 0.590375 8:-82127916 None:intergenic
CCATTCTTGTACAAGAAACG+GGG 0.603335 8:-82128642 None:intergenic
TGCAGCATATCCATGGACAA+TGG 0.606154 8:+82129213 MsG0880047114.01.T01:CDS
TGACAGACTACAGAAAAGAT+GGG 0.606912 8:+82129299 MsG0880047114.01.T01:CDS
AGTAGAAACTACTTTCAGGA+AGG 0.620156 8:+82128891 MsG0880047114.01.T01:intron
AAATATCGTTGGAAGCCACA+TGG 0.625306 8:+82128159 MsG0880047114.01.T01:CDS
GAATTCCAGGAAGGCACCAG+TGG 0.629536 8:-82129390 None:intergenic
ATTCTCTTCTGTTACTGCAG+CGG 0.630007 8:+82127957 MsG0880047114.01.T01:CDS
CTCTGGTGGTAAATGTGATG+GGG 0.640966 8:+82129159 MsG0880047114.01.T01:CDS
AAAATGTTGCACATGTTGAG+AGG 0.654333 8:+82128326 MsG0880047114.01.T01:CDS
ACTCTTTAAACAGGAACATG+TGG 0.657678 8:+82128372 MsG0880047114.01.T01:CDS
CTTGGAATTTGACAACCATG+TGG 0.659855 8:-82128174 None:intergenic
TGTACGTGTGCTATCAACTG+TGG 0.663378 8:-82128991 None:intergenic
AGTTGATAGCACACGTACAA+GGG 0.666680 8:+82128995 MsG0880047114.01.T01:CDS
CCAGGTTTCAGTGATAGCAA+AGG 0.667037 8:+82129343 MsG0880047114.01.T01:CDS
AAGAGTCTGGAATTCCAGGA+AGG 0.673363 8:-82129399 None:intergenic
GTAATGTTGAGGTAAAACAC+TGG 0.678454 8:-82129271 None:intergenic
CAAAGATATCACATGAACTG+AGG 0.682277 8:-82128020 None:intergenic
CATGTTGAGAGGTAAGAGAG+TGG 0.752288 8:+82128337 MsG0880047114.01.T01:CDS
ACTGCAGCGGAAAGAACACG+AGG 0.760490 8:+82127970 MsG0880047114.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAATAAATTAAAATGAACA+TGG - Chr8:82128247-82128266 None:intergenic 15.0%
!!! TTTGATTTTTTTTTCTTTCT+TGG + Chr8:82128276-82128295 MsG0880047114.01.T01:intron 15.0%
!! ATATATACATATAGGTTTGA+TGG + Chr8:82128299-82128318 MsG0880047114.01.T01:intron 20.0%
!! TATATACATATAGGTTTGAT+GGG + Chr8:82128300-82128319 MsG0880047114.01.T01:intron 20.0%
!! TTTCTTGGATATATACATAT+AGG + Chr8:82128291-82128310 MsG0880047114.01.T01:intron 20.0%
!! TTTCTTTCAAATTCAAAGTA+AGG + Chr8:82128489-82128508 MsG0880047114.01.T01:intron 20.0%
!!! TAGTTAAGTAATTGATTCTA+TGG + Chr8:82129108-82129127 MsG0880047114.01.T01:intron 20.0%
!!! TATAGCTCAAACAAATTTTA+TGG + Chr8:82129450-82129469 MsG0880047114.01.T01:CDS 20.0%
!!! TTAAAAGTATCATCAAGAAA+AGG - Chr8:82128102-82128121 None:intergenic 20.0%
! AAGATAGCTTTCATTTGTAA+TGG - Chr8:82129194-82129213 None:intergenic 25.0%
! AATCATGACAAGACAAAAAA+TGG + Chr8:82128785-82128804 MsG0880047114.01.T01:CDS 25.0%
! ACTGTTAATAAGTAGTTCAA+AGG + Chr8:82128841-82128860 MsG0880047114.01.T01:intron 25.0%
! AGATAGCTTTCATTTGTAAT+GGG - Chr8:82129193-82129212 None:intergenic 25.0%
! ATCATGACAAGACAAAAAAT+GGG + Chr8:82128786-82128805 MsG0880047114.01.T01:CDS 25.0%
! TTAAAATGAACATGGTACAT+TGG - Chr8:82128239-82128258 None:intergenic 25.0%
! TTGAGTTTCTTAAATATCGT+TGG + Chr8:82128148-82128167 MsG0880047114.01.T01:CDS 25.0%
!! GATATAATCATTTTCAACAC+AGG + Chr8:82128752-82128771 MsG0880047114.01.T01:CDS 25.0%
!!! ACTTTTAACTGTTTCAAGAA+TGG + Chr8:82128114-82128133 MsG0880047114.01.T01:CDS 25.0%
AATTCAAAGTAAGGATGTGT+TGG + Chr8:82128498-82128517 MsG0880047114.01.T01:intron 30.0%
AGTTCATGTGATATCTTTGA+TGG + Chr8:82128024-82128043 MsG0880047114.01.T01:CDS 30.0%
GAAACTATGTAAGAAGTGAA+TGG - Chr8:82127919-82127938 None:intergenic 30.0%
GACATGTTGTGATGTTAATT+AGG + Chr8:82128589-82128608 MsG0880047114.01.T01:intron 30.0%
TTCCATTCTTGTACAAGAAA+CGG - Chr8:82128647-82128666 None:intergenic 30.0%
! ATGTCGAATTTTACAAGGTA+GGG - Chr8:82128215-82128234 None:intergenic 30.0%
! TATGTCGAATTTTACAAGGT+AGG - Chr8:82128216-82128235 None:intergenic 30.0%
! TTGGTATGTCGAATTTTACA+AGG - Chr8:82128220-82128239 None:intergenic 30.0%
! TTTTGCTTGCTTAAGGTTTA+AGG + Chr8:82128549-82128568 MsG0880047114.01.T01:intron 30.0%
!! TTTTCTGTAGTCTGTCATTT+TGG - Chr8:82129296-82129315 None:intergenic 30.0%
AAAATGTTGCACATGTTGAG+AGG + Chr8:82128326-82128345 MsG0880047114.01.T01:CDS 35.0%
AACCTTGAATCCTGAATTCA+CGG - Chr8:82128466-82128485 None:intergenic 35.0%
ACTACACGTTTGAAAGAAAG+TGG - Chr8:82127886-82127905 None:intergenic 35.0%
ACTCTTTAAACAGGAACATG+TGG + Chr8:82128372-82128391 MsG0880047114.01.T01:CDS 35.0%
AGCATCATAATACTGAGACT+TGG - Chr8:82128732-82128751 None:intergenic 35.0%
AGTAGAAACTACTTTCAGGA+AGG + Chr8:82128891-82128910 MsG0880047114.01.T01:intron 35.0%
ATGACAGACTACAGAAAAGA+TGG + Chr8:82129298-82129317 MsG0880047114.01.T01:CDS 35.0%
CAAAGATATCACATGAACTG+AGG - Chr8:82128023-82128042 None:intergenic 35.0%
CATGAACCTGTTTATCAACA+TGG + Chr8:82128069-82128088 MsG0880047114.01.T01:CDS 35.0%
CCTTCAATTTATAGAGAACC+AGG + Chr8:82129325-82129344 MsG0880047114.01.T01:CDS 35.0%
CTATAAGTAGAGTGGAATAG+AGG - Chr8:82129435-82129454 None:intergenic 35.0%
GGGTAGAAATTATAGAACCT+TGG - Chr8:82128195-82128214 None:intergenic 35.0%
GTAATGTTGAGGTAAAACAC+TGG - Chr8:82129274-82129293 None:intergenic 35.0%
TCCATTCTTGTACAAGAAAC+GGG - Chr8:82128646-82128665 None:intergenic 35.0%
TGACAGACTACAGAAAAGAT+GGG + Chr8:82129299-82129318 MsG0880047114.01.T01:CDS 35.0%
TGTTTGAGCTATAAGTAGAG+TGG - Chr8:82129443-82129462 None:intergenic 35.0%
TTCCTCAAAGAGATACTCTT+AGG + Chr8:82128687-82128706 MsG0880047114.01.T01:CDS 35.0%
TTGCAGTAGAAACTACTTTC+AGG + Chr8:82128887-82128906 MsG0880047114.01.T01:intron 35.0%
TTGTAGGAGACTCTTTAAAC+AGG + Chr8:82128363-82128382 MsG0880047114.01.T01:CDS 35.0%
! ATAGAGCTTTTGCTTGCTTA+AGG + Chr8:82128542-82128561 MsG0880047114.01.T01:intron 35.0%
! CCTGGTTCTCTATAAATTGA+AGG - Chr8:82129328-82129347 None:intergenic 35.0%
! TGCAATCTTGGACCATTTTA+CGG - Chr8:82129369-82129388 None:intergenic 35.0%
!! ACAAAAAGCACTGAAGACAT+GGG + Chr8:82128953-82128972 MsG0880047114.01.T01:CDS 35.0%
!! TACAAAAAGCACTGAAGACA+TGG + Chr8:82128952-82128971 MsG0880047114.01.T01:CDS 35.0%
!!! CTGTCATTTTGGTAATGTTG+AGG - Chr8:82129285-82129304 None:intergenic 35.0%
AAATATCGTTGGAAGCCACA+TGG + Chr8:82128159-82128178 MsG0880047114.01.T01:CDS 40.0%
AACTCTGGTGGTAAATGTGA+TGG + Chr8:82129157-82129176 MsG0880047114.01.T01:CDS 40.0%
ACTCTGGTGGTAAATGTGAT+GGG + Chr8:82129158-82129177 MsG0880047114.01.T01:CDS 40.0%
AGCAGACTCTATGAAGTTTC+TGG + Chr8:82128437-82128456 MsG0880047114.01.T01:CDS 40.0%
ATTCTCTTCTGTTACTGCAG+CGG + Chr8:82127957-82127976 MsG0880047114.01.T01:CDS 40.0%
CACATGGTTGTCAAATTCCA+AGG + Chr8:82128175-82128194 MsG0880047114.01.T01:CDS 40.0%
CCATTCTTGTACAAGAAACG+GGG - Chr8:82128645-82128664 None:intergenic 40.0%
CTATGGCTGAAATTTGCAGA+GGG + Chr8:82129125-82129144 MsG0880047114.01.T01:intron 40.0%
CTTGGAATTTGACAACCATG+TGG - Chr8:82128177-82128196 None:intergenic 40.0%
GAAAGTGGAAAACGAAGAGA+AGG - Chr8:82127871-82127890 None:intergenic 40.0%
TATGGCTGAAATTTGCAGAG+GGG + Chr8:82129126-82129145 MsG0880047114.01.T01:intron 40.0%
TCTATGGCTGAAATTTGCAG+AGG + Chr8:82129124-82129143 MsG0880047114.01.T01:intron 40.0%
TGATAGCAAAGGCCGTAAAA+TGG + Chr8:82129354-82129373 MsG0880047114.01.T01:CDS 40.0%
TGGAAAACGAAGAGAAGGAA+TGG - Chr8:82127866-82127885 None:intergenic 40.0%
! CACGAACCATGTTGATAAAC+AGG - Chr8:82128078-82128097 None:intergenic 40.0%
! GTGATATCTTTGATGGCTCT+TGG + Chr8:82128031-82128050 MsG0880047114.01.T01:CDS 40.0%
! TCATTTTCAACACAGGACAC+TGG + Chr8:82128759-82128778 MsG0880047114.01.T01:CDS 40.0%
!! AGCCTAAGAGTATCTCTTTG+AGG - Chr8:82128692-82128711 None:intergenic 40.0%
!! AGTTGATAGCACACGTACAA+GGG + Chr8:82128995-82129014 MsG0880047114.01.T01:CDS 40.0%
!!! CGTACAAGGGTATTTTTCAC+TGG + Chr8:82129008-82129027 MsG0880047114.01.T01:CDS 40.0%
ACGAAGAGAAGGAATGGTAC+TGG - Chr8:82127860-82127879 None:intergenic 45.0%
AGGAGTTCATTCCAAGAGTC+TGG - Chr8:82129415-82129434 None:intergenic 45.0%
ATGGCTGAAATTTGCAGAGG+GGG + Chr8:82129127-82129146 MsG0880047114.01.T01:intron 45.0%
CAGGAACATGTGGCAATCTT+TGG + Chr8:82128382-82128401 MsG0880047114.01.T01:CDS 45.0%
CATGTTGAGAGGTAAGAGAG+TGG + Chr8:82128337-82128356 MsG0880047114.01.T01:CDS 45.0%
CCAGGTTTCAGTGATAGCAA+AGG + Chr8:82129343-82129362 MsG0880047114.01.T01:CDS 45.0%
CCCCGTTTCTTGTACAAGAA+TGG + Chr8:82128642-82128661 MsG0880047114.01.T01:CDS 45.0%
CGGGGATTTCACAAAGTCTA+TGG - Chr8:82128627-82128646 None:intergenic 45.0%
CTCTGGTGGTAAATGTGATG+GGG + Chr8:82129159-82129178 MsG0880047114.01.T01:CDS 45.0%
GCAGCATATCCATGGACAAT+GGG + Chr8:82129214-82129233 MsG0880047114.01.T01:CDS 45.0%
GCTATCTTGCAGCATATCCA+TGG + Chr8:82129206-82129225 MsG0880047114.01.T01:CDS 45.0%
GGTAAGAGAGTGGTGTTTGT+AGG + Chr8:82128347-82128366 MsG0880047114.01.T01:CDS 45.0%
TCGACAATTCCCATTGTCCA+TGG - Chr8:82129226-82129245 None:intergenic 45.0%
TGCAGCATATCCATGGACAA+TGG + Chr8:82129213-82129232 MsG0880047114.01.T01:CDS 45.0%
TGTACGTGTGCTATCAACTG+TGG - Chr8:82128994-82129013 None:intergenic 45.0%
TTCCTGGAATTCCAGACTCT+TGG + Chr8:82129401-82129420 MsG0880047114.01.T01:CDS 45.0%
! AAGAGTCTGGAATTCCAGGA+AGG - Chr8:82129402-82129421 None:intergenic 45.0%
! ACTGAAGACATGGGCTAAGT+GGG + Chr8:82128962-82128981 MsG0880047114.01.T01:CDS 45.0%
! CCTTTGCTATCACTGAAACC+TGG - Chr8:82129346-82129365 None:intergenic 45.0%
! TTCCAAGAGTCTGGAATTCC+AGG - Chr8:82129406-82129425 None:intergenic 45.0%
! TTTTCAACACAGGACACTGG+TGG + Chr8:82128762-82128781 MsG0880047114.01.T01:CDS 45.0%
!! CAGTTGATAGCACACGTACA+AGG + Chr8:82128994-82129013 MsG0880047114.01.T01:CDS 45.0%
AAATTTGCAGAGGGGGGCAA+TGG + Chr8:82129134-82129153 MsG0880047114.01.T01:intron 50.0%
CGCCGTGAATTCAGGATTCA+AGG + Chr8:82128461-82128480 MsG0880047114.01.T01:CDS 50.0%
TGGCTGAAATTTGCAGAGGG+GGG + Chr8:82129128-82129147 MsG0880047114.01.T01:intron 50.0%
TTTCTGGACGCCGTGAATTC+AGG + Chr8:82128453-82128472 MsG0880047114.01.T01:CDS 50.0%
! CACTGAAGACATGGGCTAAG+TGG + Chr8:82128961-82128980 MsG0880047114.01.T01:CDS 50.0%
ACTGCAGCGGAAAGAACACG+AGG + Chr8:82127970-82127989 MsG0880047114.01.T01:CDS 55.0%
TGGTCCAAGATTGCAGCCAC+TGG + Chr8:82129374-82129393 MsG0880047114.01.T01:CDS 55.0%
!! GAATTCCAGGAAGGCACCAG+TGG - Chr8:82129393-82129412 None:intergenic 55.0%
AGAGGGGGGCAATGGAACTC+TGG + Chr8:82129142-82129161 MsG0880047114.01.T01:intron 60.0%
GGCACCAGTGGCTGCAATCT+TGG - Chr8:82129381-82129400 None:intergenic 60.0%
GGGGGGCAATGGAACTCTGG+TGG + Chr8:82129145-82129164 MsG0880047114.01.T01:CDS 65.0%
TGCAGCCACTGGTGCCTTCC+TGG + Chr8:82129385-82129404 MsG0880047114.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr8 gene 82127853 82129489 82127853 ID=MsG0880047114.01;Name=MsG0880047114.01
Chr8 mRNA 82127853 82129489 82127853 ID=MsG0880047114.01.T01;Parent=MsG0880047114.01;Name=MsG0880047114.01.T01;_AED=0.39;_eAED=0.40;_QI=0|0|0|1|1|1|5|0|412
Chr8 exon 82127853 82128196 82127853 ID=MsG0880047114.01.T01:exon:35034;Parent=MsG0880047114.01.T01
Chr8 exon 82128313 82128505 82128313 ID=MsG0880047114.01.T01:exon:35035;Parent=MsG0880047114.01.T01
Chr8 exon 82128611 82128807 82128611 ID=MsG0880047114.01.T01:exon:35036;Parent=MsG0880047114.01.T01
Chr8 exon 82128893 82129051 82128893 ID=MsG0880047114.01.T01:exon:35037;Parent=MsG0880047114.01.T01
Chr8 exon 82129144 82129489 82129144 ID=MsG0880047114.01.T01:exon:35038;Parent=MsG0880047114.01.T01
Chr8 CDS 82127853 82128196 82127853 ID=MsG0880047114.01.T01:cds;Parent=MsG0880047114.01.T01
Chr8 CDS 82128313 82128505 82128313 ID=MsG0880047114.01.T01:cds;Parent=MsG0880047114.01.T01
Chr8 CDS 82128611 82128807 82128611 ID=MsG0880047114.01.T01:cds;Parent=MsG0880047114.01.T01
Chr8 CDS 82128893 82129051 82128893 ID=MsG0880047114.01.T01:cds;Parent=MsG0880047114.01.T01
Chr8 CDS 82129144 82129489 82129144 ID=MsG0880047114.01.T01:cds;Parent=MsG0880047114.01.T01
Gene Sequence

>MsG0880047114.01.T01

ATGACCAGTACCATTCCTTCTCTTCGTTTTCCACTTTCTTTCAAACGTGTAGTGATACATGCACCATTCACTTCTTACATAGTTTCAGAAGCACAGAGCAACACATTCTCTTCTGTTACTGCAGCGGAAAGAACACGAGGAAATTCAACAGATAGTGACTCACAAGACCTCAGTTCATGTGATATCTTTGATGGCTCTTGGATTCAAGATGATTCTCATGAACCTGTTTATCAACATGGTTCGTGTCCTTTTCTTGATGATACTTTTAACTGTTTCAAGAATGGACGTTCAGATTTTGAGTTTCTTAAATATCGTTGGAAGCCACATGGTTGTCAAATTCCAAGGTTTGATGGGTTGAAAATGTTGCACATGTTGAGAGGTAAGAGAGTGGTGTTTGTAGGAGACTCTTTAAACAGGAACATGTGGCAATCTTTGGTGTGTGCTTTGAGAGCTTCTCTTAAAGATAAGAGCAGACTCTATGAAGTTTCTGGACGCCGTGAATTCAGGATTCAAGGTTTCTTTTCTTTCAAATTCAAAGATTATGAATGCTCCATAGACTTTGTGAAATCCCCGTTTCTTGTACAAGAATGGAAAGTGTCACGTGACGATGAAGTTCCTCAAAGAGATACTCTTAGGCTTGACATGATTCAAGCTTCCAAGTCTCAGTATTATGATGCTGATATAATCATTTTCAACACAGGACACTGGTGGAATCATGACAAGACAAAAAATGGTAGAAACTACTTTCAGGAAGGTAATCATGTCTATGACAGATTAGAAGTTTCTGAAGCATTACAAAAAGCACTGAAGACATGGGCTAAGTGGGTTGATTCCACAGTTGATAGCACACGTACAAGGGTATTTTTCACTGGATTTTCAGCTTCTCACTACAAAGGGGGGCAATGGAACTCTGGTGGTAAATGTGATGGGGAAAGACAACCCATTACAAATGAAAGCTATCTTGCAGCATATCCATGGACAATGGGAATTGTCGAAGATGTTATAGCTAAGATGAAGACGCCAGTGTTTTACCTCAACATTACCAAAATGACAGACTACAGAAAAGATGGGCATCCTTCAATTTATAGAGAACCAGGTTTCAGTGATAGCAAAGGCCGTAAAATGGTCCAAGATTGCAGCCACTGGTGCCTTCCTGGAATTCCAGACTCTTGGAATGAACTCCTCTATTCCACTCTACTTATAGCTCAAACAAATTTTATGGCTTCTACAAATTATTAA
Protein sequence

>MsG0880047114.01.T01

MTSTIPSLRFPLSFKRVVIHAPFTSYIVSEAQSNTFSSVTAAERTRGNSTDSDSQDLSSCDIFDGSWIQDDSHEPVYQHGSCPFLDDTFNCFKNGRSDFEFLKYRWKPHGCQIPRFDGLKMLHMLRGKRVVFVGDSLNRNMWQSLVCALRASLKDKSRLYEVSGRREFRIQGFFSFKFKDYECSIDFVKSPFLVQEWKVSRDDEVPQRDTLRLDMIQASKSQYYDADIIIFNTGHWWNHDKTKNGRNYFQEGNHVYDRLEVSEALQKALKTWAKWVDSTVDSTRTRVFFTGFSASHYKGGQWNSGGKCDGERQPITNESYLAAYPWTMGIVEDVIAKMKTPVFYLNITKMTDYRKDGHPSIYREPGFSDSKGRKMVQDCSHWCLPGIPDSWNELLYSTLLIAQTNFMASTNY*