AlfalfaGEDB | MsG0880047188.01

M. sativa cultivar ZhongmuNo.1 / MsG0880047188.01

KEGG: -
GO: GO:0004857 GO:0043086
PFAM: PF04043

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047188.01.T01	XP_003609812.1	96.045	177	3	1	1	177	1	173	7.75E-118	341

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047188.01.T01	F4HWQ8	45.775	142	77	0	32	173	21	162	2.36E-41	140

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047188.01.T01	G7JP02	96.045	177	3	1	1	177	1	173	3.70e-118	341

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000114.01	MsG0880047188.01	0.809592	1.753557e-50	1.033834e-47
MsG0680034767.01	MsG0880047188.01	0.800246	1.576929e-48	7.306876e-46
MsG0480019007.01	MsG0880047188.01	0.809421	1.907823e-50	1.119677e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047188.01.T01	MTR_4g122750	96.045	177	3	1	1	177	1	173	9.39e-122	341
MsG0880047188.01.T01	MTR_4g122760	73.333	180	41	3	1	177	1	176	3.65e-86	251
MsG0880047188.01.T01	MTR_4g122890	53.073	179	78	4	1	177	1	175	1.02e-59	184
MsG0880047188.01.T01	MTR_4g122800	50.549	182	85	1	1	177	1	182	1.43e-57	179
MsG0880047188.01.T01	MTR_4g007280	51.852	108	50	2	72	177	1	108	5.20e-28	101
MsG0880047188.01.T01	MTR_4g122780	41.722	151	72	3	43	177	8	158	6.00e-26	97.8
MsG0880047188.01.T01	MTR_2g033630	35.673	171	98	4	19	177	6	176	2.39e-21	86.3
MsG0880047188.01.T01	MTR_4g122740	34.810	158	98	3	25	177	20	177	2.71e-19	80.9
MsG0880047188.01.T01	MTR_4g122660	28.729	181	117	5	3	177	2	176	5.46e-18	77.4
MsG0880047188.01.T01	MTR_4g122720	30.612	147	98	2	35	177	39	185	2.00e-14	68.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047188.01.T01	AT1G47960	46.575	146	78	0	32	177	21	166	2.39e-45	147
MsG0880047188.01.T01	AT1G47960	45.775	142	77	0	32	173	21	162	2.40e-42	140
MsG0880047188.01.T01	AT3G17152	30.857	175	119	1	4	176	1	175	3.88e-18	77.8
MsG0880047188.01.T01	AT3G17150	30.769	143	97	1	37	177	39	181	9.66e-15	68.9

Find 24 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGAATGCAATTTGCATAATT+TGG	0.133285	8:+83106133	None:intergenic
GCACCATCTTCTGCAAATTT+AGG	0.160091	8:+83105884	None:intergenic
ATCAAATTAGCATTGGATTT+TGG	0.186303	8:+83106178	None:intergenic
TTGAGAGCTGCAACAGATTT+TGG	0.207656	8:+83105917	None:intergenic
CAAAATCAATCAGTTGATTA+AGG	0.217105	8:-83106011	MsG0880047188.01.T01:CDS
ACATCTGCATCAGAGCTTCT+TGG	0.293970	8:+83106094	None:intergenic
TGGTAGATACAAAGCAATTT+TGG	0.313317	8:-83105951	MsG0880047188.01.T01:CDS
AATGCTAATGCTAATGCTAA+TGG	0.350974	8:+83106250	None:intergenic
GTTTAAGGCAGTGTTTGCTT+TGG	0.395895	8:+83106033	None:intergenic
AAAATCAATCAGTTGATTAA+GGG	0.419264	8:-83106010	MsG0880047188.01.T01:CDS
AGCTCTGATGCAGATGTAAC+AGG	0.425806	8:-83106088	MsG0880047188.01.T01:CDS
GAAGCCACTACTTGTGAGAA+TGG	0.463193	8:-83105845	MsG0880047188.01.T01:CDS
TTGCTGTATCAAATTAGCAT+TGG	0.473617	8:+83106171	None:intergenic
GCAATATTCTCATCAGAGAT+TGG	0.475595	8:+83105806	None:intergenic
TAAGGGGAGCCATGATCAAA+AGG	0.485227	8:-83105993	MsG0880047188.01.T01:CDS
GATCCTAAATTTGCAGAAGA+TGG	0.485944	8:-83105887	MsG0880047188.01.T01:CDS
GAATCCATTCTCACAAGTAG+TGG	0.525508	8:+83105841	None:intergenic
ACTTGTGAGAATGGATTCAA+AGG	0.538015	8:-83105836	MsG0880047188.01.T01:CDS
GAAGCTTTGAATTCATGTGC+TGG	0.550109	8:-83105971	MsG0880047188.01.T01:CDS
AACAGGTCTAGCACTAATCA+TGG	0.552410	8:-83106071	MsG0880047188.01.T01:CDS
CTTGTGAGAATGGATTCAAA+GGG	0.562306	8:-83105835	MsG0880047188.01.T01:CDS
AAATCAATCAGTTGATTAAG+GGG	0.571558	8:-83106009	MsG0880047188.01.T01:CDS
TCTGTTGCAGCTCTCAAACA+AGG	0.592971	8:-83105911	MsG0880047188.01.T01:CDS
AGGTCTAGCACTAATCATGG+TGG	0.723735	8:-83106068	MsG0880047188.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAATCAATCAGTTGATTAA+GGG	-	Chr8:83106009-83106028	MsG0880047188.01.T01:CDS	20.0%
!	AAATCAATCAGTTGATTAAG+GGG	-	Chr8:83106010-83106029	MsG0880047188.01.T01:CDS	25.0%
!	CAAAATCAATCAGTTGATTA+AGG	-	Chr8:83106008-83106027	MsG0880047188.01.T01:CDS	25.0%
!	TGAATGCAATTTGCATAATT+TGG	+	Chr8:83105889-83105908	None:intergenic	25.0%
!!!	ATCAAATTAGCATTGGATTT+TGG	+	Chr8:83105844-83105863	None:intergenic	25.0%
!!!	CAACTGATTGATTTTGTTTA+AGG	+	Chr8:83106004-83106023	None:intergenic	25.0%
	AATGCTAATGCTAATGCTAA+TGG	+	Chr8:83105772-83105791	None:intergenic	30.0%
	TTGCTGTATCAAATTAGCAT+TGG	+	Chr8:83105851-83105870	None:intergenic	30.0%
!	TGGTAGATACAAAGCAATTT+TGG	-	Chr8:83106068-83106087	MsG0880047188.01.T01:CDS	30.0%
!!	AATTAGCATTGGATTTTGGA+TGG	+	Chr8:83105840-83105859	None:intergenic	30.0%
	GATCCTAAATTTGCAGAAGA+TGG	-	Chr8:83106132-83106151	MsG0880047188.01.T01:CDS	35.0%
	GCAATATTCTCATCAGAGAT+TGG	+	Chr8:83106216-83106235	None:intergenic	35.0%
!	ACTTGTGAGAATGGATTCAA+AGG	-	Chr8:83106183-83106202	MsG0880047188.01.T01:CDS	35.0%
!	CTTGTGAGAATGGATTCAAA+GGG	-	Chr8:83106184-83106203	MsG0880047188.01.T01:CDS	35.0%
!	TCAAAGCTTCCTTTTGATCA+TGG	+	Chr8:83106038-83106057	None:intergenic	35.0%
	AACAGGTCTAGCACTAATCA+TGG	-	Chr8:83105948-83105967	MsG0880047188.01.T01:CDS	40.0%
	GAATCCATTCTCACAAGTAG+TGG	+	Chr8:83106181-83106200	None:intergenic	40.0%
	GCACCATCTTCTGCAAATTT+AGG	+	Chr8:83106138-83106157	None:intergenic	40.0%
!	GTTTAAGGCAGTGTTTGCTT+TGG	+	Chr8:83105989-83106008	None:intergenic	40.0%
!	TTGAGAGCTGCAACAGATTT+TGG	+	Chr8:83106105-83106124	None:intergenic	40.0%
!!	GAAGCTTTGAATTCATGTGC+TGG	-	Chr8:83106048-83106067	MsG0880047188.01.T01:CDS	40.0%
	ACATCTGCATCAGAGCTTCT+TGG	+	Chr8:83105928-83105947	None:intergenic	45.0%
	AGGTCTAGCACTAATCATGG+TGG	-	Chr8:83105951-83105970	MsG0880047188.01.T01:CDS	45.0%
	GAAGCCACTACTTGTGAGAA+TGG	-	Chr8:83106174-83106193	MsG0880047188.01.T01:CDS	45.0%
	TAAGGGGAGCCATGATCAAA+AGG	-	Chr8:83106026-83106045	MsG0880047188.01.T01:CDS	45.0%
	TCTGTTGCAGCTCTCAAACA+AGG	-	Chr8:83106108-83106127	MsG0880047188.01.T01:CDS	45.0%
!	AGCTCTGATGCAGATGTAAC+AGG	-	Chr8:83105931-83105950	MsG0880047188.01.T01:CDS	45.0%

Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	83105754	83106287	83105754	ID=MsG0880047188.01;Name=MsG0880047188.01
Chr8	mRNA	83105754	83106287	83105754	ID=MsG0880047188.01.T01;Parent=MsG0880047188.01;Name=MsG0880047188.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|177
Chr8	exon	83105754	83106287	83105754	ID=MsG0880047188.01.T01:exon:11724;Parent=MsG0880047188.01.T01
Chr8	CDS	83105754	83106287	83105754	ID=MsG0880047188.01.T01:cds;Parent=MsG0880047188.01.T01

Gene Sequence

>MsG0880047188.01.T01

ATGACAAAGATGAATCCATTAGCATTAGCATTAGCATTAGCTATCATCTTGTGCACTATTGTTTCAAGTCACTGCATAATTATCCATCCAAAATCCAATGCTAATTTGATACAGCAAACTTGCAAACAAACTCCAAATTATGCAAATTGCATTCACTATCTCAAATCTGATCCAAGAAGCTCTGATGCAGATGTAACAGGTCTAGCACTAATCATGGTGGACATAATCAAATCCAAAGCAAACACTGCCTTAAACAAAATCAATCAGTTGATTAAGGGGAGCCATGATCAAAAGGAAGCTTTGAATTCATGTGCTGGTAGATACAAAGCAATTTTGGTAGCTGATGTGCCAAAATCTGTTGCAGCTCTCAAACAAGGAGATCCTAAATTTGCAGAAGATGGTGCAAATGATGTTGCAATTGAAGCCACTACTTGTGAGAATGGATTCAAAGGGAAATCACCAATCTCTGATGAGAATATTGCTATGCATGATGTAGCAGTCATAACAGCAGCTATTGTTAAACAATTGCTCTAG

Protein sequence

>MsG0880047188.01.T01

MTKMNPLALALALAIILCTIVSSHCIIIHPKSNANLIQQTCKQTPNYANCIHYLKSDPRSSDADVTGLALIMVDIIKSKANTALNKINQLIKGSHDQKEALNSCAGRYKAILVADVPKSVAALKQGDPKFAEDGANDVAIEATTCENGFKGKSPISDENIAMHDVAVITAAIVKQLL*