Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047349.01.T01 | KEH32456.1 | 87.143 | 210 | 23 | 1 | 20 | 229 | 2 | 207 | 1.53E-118 | 347 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047349.01.T01 | Q9SYD8 | 41.429 | 210 | 114 | 4 | 20 | 228 | 1 | 202 | 2.30E-46 | 156 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047349.01.T01 | A0A072USZ1 | 87.143 | 210 | 23 | 1 | 20 | 229 | 2 | 207 | 7.33e-119 | 347 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0680030882.01 | MsG0880047349.01 | 0.801034 | 1.089008e-48 | 5.148342e-46 |
| MsG0680034230.01 | MsG0880047349.01 | 0.804023 | 2.635347e-49 | 1.344426e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047349.01.T01 | MTR_4g125560 | 87.143 | 210 | 23 | 1 | 20 | 229 | 2 | 207 | 1.86e-122 | 347 |
| MsG0880047349.01.T01 | MTR_4g125530 | 45.854 | 205 | 99 | 6 | 24 | 224 | 52 | 248 | 2.26e-58 | 186 |
| MsG0880047349.01.T01 | MTR_0121s0060 | 38.605 | 215 | 113 | 7 | 24 | 228 | 52 | 257 | 1.98e-43 | 147 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047349.01.T01 | AT1G51360 | 41.429 | 210 | 114 | 4 | 20 | 228 | 1 | 202 | 2.34e-47 | 156 |
| MsG0880047349.01.T01 | AT2G31670 | 39.252 | 214 | 111 | 6 | 23 | 228 | 48 | 250 | 8.54e-47 | 156 |
Find 57 sgRNAs with CRISPR-Local
Find 71 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTGATTCTGATGAGAAATT+TGG | 0.119057 | 8:-85075046 | MsG0880047349.01.T01:CDS |
| ACATCCTTTCTTTCTGTTGT+TGG | 0.285441 | 8:+85075614 | None:intergenic |
| TCACATGAATGATAAGATTA+AGG | 0.311885 | 8:-85075017 | MsG0880047349.01.T01:CDS |
| CTTCAAGTTGAAGGAGGGTT+TGG | 0.321534 | 8:-85075227 | MsG0880047349.01.T01:CDS |
| AGAGCCACTTCACTTAACTA+TGG | 0.332780 | 8:-85075488 | MsG0880047349.01.T01:CDS |
| CTGATTCTGATGAGAAATTT+GGG | 0.334720 | 8:-85075045 | MsG0880047349.01.T01:CDS |
| TCGAGCATGTGGTGTTGTTC+AGG | 0.352637 | 8:-85075582 | MsG0880047349.01.T01:CDS |
| TGATAAGATTAAGGAAAATC+TGG | 0.365828 | 8:-85075008 | MsG0880047349.01.T01:CDS |
| TCCAGGTTTAACCGAATTGC+AGG | 0.367963 | 8:-85075071 | MsG0880047349.01.T01:CDS |
| TTCAAGTTGAAGGAGGGTTT+GGG | 0.376642 | 8:-85075226 | MsG0880047349.01.T01:CDS |
| TTCTCTCCTGGTAGAGCTAA+AGG | 0.386910 | 8:-85075121 | MsG0880047349.01.T01:CDS |
| ATGAAACCATGGCTCTTGAT+TGG | 0.394542 | 8:-85075312 | MsG0880047349.01.T01:CDS |
| ACTGAAAGATGAAAGGGAAT+TGG | 0.400879 | 8:+85075441 | None:intergenic |
| CTGGACACTGTACTGGTCCT+TGG | 0.413853 | 8:-85074989 | MsG0880047349.01.T01:CDS |
| AGAGCTAAAGGATTCTCTAT+TGG | 0.420200 | 8:-85075109 | MsG0880047349.01.T01:CDS |
| AGAATCAGCTTCCTGCAATT+CGG | 0.430055 | 8:+85075060 | None:intergenic |
| GCCATGGTTTCATCAACTAT+AGG | 0.450016 | 8:+85075322 | None:intergenic |
| AAGGAAAGTATTGAATATGC+AGG | 0.456887 | 8:-85075649 | MsG0880047349.01.T01:CDS |
| TGGACACTGTACTGGTCCTT+GGG | 0.463405 | 8:-85074988 | MsG0880047349.01.T01:intron |
| AGTTGTGTTCTTCAAGTTGA+AGG | 0.472243 | 8:-85075236 | MsG0880047349.01.T01:CDS |
| GGAAAATCTGGACACTGTAC+TGG | 0.477993 | 8:-85074996 | MsG0880047349.01.T01:CDS |
| TCCTGCAATTCGGTTAAACC+TGG | 0.483602 | 8:+85075070 | None:intergenic |
| CGAGGTTCACGGTAAGGACT+TGG | 0.489470 | 8:-85075284 | MsG0880047349.01.T01:CDS |
| GAAAGATGAAAGGGAATTGG+AGG | 0.496529 | 8:+85075444 | None:intergenic |
| TGAAACCATGGCTCTTGATT+GGG | 0.498755 | 8:-85075311 | MsG0880047349.01.T01:CDS |
| GATTGGGTCACCGAGGTTCA+CGG | 0.504028 | 8:-85075295 | MsG0880047349.01.T01:CDS |
| AGAACCAACAACAGAAAGAA+AGG | 0.506793 | 8:-85075618 | MsG0880047349.01.T01:CDS |
| TGCAGTGCACCCTACACACT+TGG | 0.533139 | 8:-85075365 | MsG0880047349.01.T01:CDS |
| ACAATCAGCGATTGATTCCA+TGG | 0.534420 | 8:-85075542 | MsG0880047349.01.T01:CDS |
| GTGTGTAGGGTGCACTGCAT+AGG | 0.548626 | 8:+85075369 | None:intergenic |
| GAGCCACTTCACTTAACTAT+GGG | 0.556132 | 8:-85075487 | MsG0880047349.01.T01:CDS |
| GGTCACCGAGGTTCACGGTA+AGG | 0.556416 | 8:-85075290 | MsG0880047349.01.T01:CDS |
| TGATGACAGCCAAGTGTGTA+GGG | 0.562820 | 8:+85075356 | None:intergenic |
| AGTGAAACTGAAAGATGAAA+GGG | 0.568571 | 8:+85075435 | None:intergenic |
| AAGCTGTTCAGTTTAACTGT+GGG | 0.568912 | 8:-85075150 | MsG0880047349.01.T01:CDS |
| GTGTTCTTCAAGTTGAAGGA+GGG | 0.573144 | 8:-85075232 | MsG0880047349.01.T01:CDS |
| ATGGAATCAATCGCTGATTG+TGG | 0.574412 | 8:+85075544 | None:intergenic |
| TCACTTAACTATGGGTCCGT+TGG | 0.580187 | 8:-85075479 | MsG0880047349.01.T01:CDS |
| GGAATTCACTTGCTTCACCA+TGG | 0.585437 | 8:+85075525 | None:intergenic |
| GAGAATCCTTTAGCTCTACC+AGG | 0.585556 | 8:+85075115 | None:intergenic |
| TGTGTTCTTCAAGTTGAAGG+AGG | 0.590785 | 8:-85075233 | MsG0880047349.01.T01:CDS |
| AAAGCTGTTCAGTTTAACTG+TGG | 0.598480 | 8:-85075151 | MsG0880047349.01.T01:CDS |
| GAGTGAAACTGAAAGATGAA+AGG | 0.600199 | 8:+85075434 | None:intergenic |
| GTTATCAATAATCGAGCATG+TGG | 0.614479 | 8:-85075593 | MsG0880047349.01.T01:CDS |
| ATAGGCATGGAGATCTTCGT+TGG | 0.619096 | 8:+85075387 | None:intergenic |
| AGGAAAGTATTGAATATGCA+GGG | 0.623624 | 8:-85075648 | MsG0880047349.01.T01:CDS |
| TTGATGACAGCCAAGTGTGT+AGG | 0.625608 | 8:+85075355 | None:intergenic |
| GCCTATAGTTGATGAAACCA+TGG | 0.626475 | 8:-85075323 | MsG0880047349.01.T01:CDS |
| GGACCCATAGTTAAGTGAAG+TGG | 0.629855 | 8:+85075484 | None:intergenic |
| TAGGGTGCACTGCATAGGCA+TGG | 0.636624 | 8:+85075374 | None:intergenic |
| GAGGATTGAATACTTACCAA+CGG | 0.640198 | 8:+85075463 | None:intergenic |
| GGTGACCCAATCAAGAGCCA+TGG | 0.645022 | 8:+85075306 | None:intergenic |
| GGCTCTTGATTGGGTCACCG+AGG | 0.651959 | 8:-85075302 | MsG0880047349.01.T01:CDS |
| CAAGTCCTTACCGTGAACCT+CGG | 0.655186 | 8:+85075285 | None:intergenic |
| AAGGACTTGGTTCTACAACA+TGG | 0.659065 | 8:-85075271 | MsG0880047349.01.T01:CDS |
| TGGCTCTTCAAGAGAGACAA+GGG | 0.659348 | 8:+85075504 | None:intergenic |
| GTGGCTCTTCAAGAGAGACA+AGG | 0.665275 | 8:+85075503 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AACTAACATCACATTACAAT+GGG | + | Chr8:85075622-85075641 | None:intergenic | 25.0% |
| ! | ATTGTAATGTGATGTTAGTT+AGG | - | Chr8:85075622-85075641 | MsG0880047349.01.T01:CDS | 25.0% |
| ! | TAACTAACATCACATTACAA+TGG | + | Chr8:85075623-85075642 | None:intergenic | 25.0% |
| ! | TCACATGAATGATAAGATTA+AGG | - | Chr8:85075484-85075503 | MsG0880047349.01.T01:CDS | 25.0% |
| ! | TGATAAGATTAAGGAAAATC+TGG | - | Chr8:85075493-85075512 | MsG0880047349.01.T01:CDS | 25.0% |
| AGTGAAACTGAAAGATGAAA+GGG | + | Chr8:85075069-85075088 | None:intergenic | 30.0% | |
| ATATCTCATCAGAATGATGT+TGG | + | Chr8:85075554-85075573 | None:intergenic | 30.0% | |
| CATTAATTCAACTAGCAAAC+AGG | + | Chr8:85075596-85075615 | None:intergenic | 30.0% | |
| CTGATTCTGATGAGAAATTT+GGG | - | Chr8:85075456-85075475 | MsG0880047349.01.T01:CDS | 30.0% | |
| TATCTCATCAGAATGATGTT+GGG | + | Chr8:85075553-85075572 | None:intergenic | 30.0% | |
| !! | AAGGAAAGTATTGAATATGC+AGG | - | Chr8:85074852-85074871 | MsG0880047349.01.T01:CDS | 30.0% |
| !! | AGGAAAGTATTGAATATGCA+GGG | - | Chr8:85074853-85074872 | MsG0880047349.01.T01:CDS | 30.0% |
| !! | AGTGAAAAATGAGGTTTTGA+AGG | - | Chr8:85075304-85075323 | MsG0880047349.01.T01:CDS | 30.0% |
| !!! | GTGAAAAATGAGGTTTTGAA+GGG | - | Chr8:85075305-85075324 | MsG0880047349.01.T01:CDS | 30.0% |
| AAAGCTGTTCAGTTTAACTG+TGG | - | Chr8:85075350-85075369 | MsG0880047349.01.T01:CDS | 35.0% | |
| AAGCTGTTCAGTTTAACTGT+GGG | - | Chr8:85075351-85075370 | MsG0880047349.01.T01:CDS | 35.0% | |
| ACATCCTTTCTTTCTGTTGT+TGG | + | Chr8:85074890-85074909 | None:intergenic | 35.0% | |
| ACTGAAAGATGAAAGGGAAT+TGG | + | Chr8:85075063-85075082 | None:intergenic | 35.0% | |
| AGAACCAACAACAGAAAGAA+AGG | - | Chr8:85074883-85074902 | MsG0880047349.01.T01:intron | 35.0% | |
| AGAGCTAAAGGATTCTCTAT+TGG | - | Chr8:85075392-85075411 | MsG0880047349.01.T01:CDS | 35.0% | |
| GAGGATTGAATACTTACCAA+CGG | + | Chr8:85075041-85075060 | None:intergenic | 35.0% | |
| GAGTGAAACTGAAAGATGAA+AGG | + | Chr8:85075070-85075089 | None:intergenic | 35.0% | |
| GTTATCAATAATCGAGCATG+TGG | - | Chr8:85074908-85074927 | MsG0880047349.01.T01:intron | 35.0% | |
| TAAACCTGGAAAAACTGCAA+CGG | + | Chr8:85075420-85075439 | None:intergenic | 35.0% | |
| ! | GCTGATTCTGATGAGAAATT+TGG | - | Chr8:85075455-85075474 | MsG0880047349.01.T01:CDS | 35.0% |
| !! | AGTTGTGTTCTTCAAGTTGA+AGG | - | Chr8:85075265-85075284 | MsG0880047349.01.T01:CDS | 35.0% |
| AGAATCAGCTTCCTGCAATT+CGG | + | Chr8:85075444-85075463 | None:intergenic | 40.0% | |
| AGAGCCACTTCACTTAACTA+TGG | - | Chr8:85075013-85075032 | MsG0880047349.01.T01:CDS | 40.0% | |
| ATGAAACCATGGCTCTTGAT+TGG | - | Chr8:85075189-85075208 | MsG0880047349.01.T01:CDS | 40.0% | |
| GAAAGATGAAAGGGAATTGG+AGG | + | Chr8:85075060-85075079 | None:intergenic | 40.0% | |
| GAGCCACTTCACTTAACTAT+GGG | - | Chr8:85075014-85075033 | MsG0880047349.01.T01:CDS | 40.0% | |
| GCCATGGTTTCATCAACTAT+AGG | + | Chr8:85075182-85075201 | None:intergenic | 40.0% | |
| GGGAGAAGAAGTGAAAAATG+AGG | - | Chr8:85075295-85075314 | MsG0880047349.01.T01:CDS | 40.0% | |
| TGAAACCATGGCTCTTGATT+GGG | - | Chr8:85075190-85075209 | MsG0880047349.01.T01:CDS | 40.0% | |
| TGTGGGAAAAATTTCTCTCC+TGG | - | Chr8:85075368-85075387 | MsG0880047349.01.T01:CDS | 40.0% | |
| ! | ACAATCAGCGATTGATTCCA+TGG | - | Chr8:85074959-85074978 | MsG0880047349.01.T01:intron | 40.0% |
| ! | ATGGAATCAATCGCTGATTG+TGG | + | Chr8:85074960-85074979 | None:intergenic | 40.0% |
| !! | AAGGACTTGGTTCTACAACA+TGG | - | Chr8:85075230-85075249 | MsG0880047349.01.T01:CDS | 40.0% |
| !! | GCCTATAGTTGATGAAACCA+TGG | - | Chr8:85075178-85075197 | MsG0880047349.01.T01:CDS | 40.0% |
| !! | GTGTTCTTCAAGTTGAAGGA+GGG | - | Chr8:85075269-85075288 | MsG0880047349.01.T01:CDS | 40.0% |
| !! | TGTGTTCTTCAAGTTGAAGG+AGG | - | Chr8:85075268-85075287 | MsG0880047349.01.T01:CDS | 40.0% |
| !! | TTCAAGTTGAAGGAGGGTTT+GGG | - | Chr8:85075275-85075294 | MsG0880047349.01.T01:CDS | 40.0% |
| !!! | GAGGTTTTGAAGGGTATAAG+TGG | - | Chr8:85075314-85075333 | MsG0880047349.01.T01:CDS | 40.0% |
| ATAGGCATGGAGATCTTCGT+TGG | + | Chr8:85075117-85075136 | None:intergenic | 45.0% | |
| GGAAAATCTGGACACTGTAC+TGG | - | Chr8:85075505-85075524 | MsG0880047349.01.T01:CDS | 45.0% | |
| GGAATTCACTTGCTTCACCA+TGG | + | Chr8:85074979-85074998 | None:intergenic | 45.0% | |
| GGACCCATAGTTAAGTGAAG+TGG | + | Chr8:85075020-85075039 | None:intergenic | 45.0% | |
| TCACTTAACTATGGGTCCGT+TGG | - | Chr8:85075022-85075041 | MsG0880047349.01.T01:CDS | 45.0% | |
| TCCAGGTTTAACCGAATTGC+AGG | - | Chr8:85075430-85075449 | MsG0880047349.01.T01:CDS | 45.0% | |
| TCCTGCAATTCGGTTAAACC+TGG | + | Chr8:85075434-85075453 | None:intergenic | 45.0% | |
| TGATGACAGCCAAGTGTGTA+GGG | + | Chr8:85075148-85075167 | None:intergenic | 45.0% | |
| TGGCTCTTCAAGAGAGACAA+GGG | + | Chr8:85075000-85075019 | None:intergenic | 45.0% | |
| TTCTCTCCTGGTAGAGCTAA+AGG | - | Chr8:85075380-85075399 | MsG0880047349.01.T01:CDS | 45.0% | |
| ! | TTGATGACAGCCAAGTGTGT+AGG | + | Chr8:85075149-85075168 | None:intergenic | 45.0% |
| !! | CTTCAAGTTGAAGGAGGGTT+TGG | - | Chr8:85075274-85075293 | MsG0880047349.01.T01:CDS | 45.0% |
| !! | GAGAATCCTTTAGCTCTACC+AGG | + | Chr8:85075389-85075408 | None:intergenic | 45.0% |
| CAAGTCCTTACCGTGAACCT+CGG | + | Chr8:85075219-85075238 | None:intergenic | 50.0% | |
| GGCTATGCATCACATACCCA+AGG | + | Chr8:85075532-85075551 | None:intergenic | 50.0% | |
| GTGGCTCTTCAAGAGAGACA+AGG | + | Chr8:85075001-85075020 | None:intergenic | 50.0% | |
| ! | GGTTCCGTTGCAGTTTTTCC+AGG | - | Chr8:85075413-85075432 | MsG0880047349.01.T01:CDS | 50.0% |
| ! | TGGACACTGTACTGGTCCTT+GGG | - | Chr8:85075513-85075532 | MsG0880047349.01.T01:CDS | 50.0% |
| !! | TCGAGCATGTGGTGTTGTTC+AGG | - | Chr8:85074919-85074938 | MsG0880047349.01.T01:intron | 50.0% |
| CGAGGTTCACGGTAAGGACT+TGG | - | Chr8:85075217-85075236 | MsG0880047349.01.T01:CDS | 55.0% | |
| GATTGGGTCACCGAGGTTCA+CGG | - | Chr8:85075206-85075225 | MsG0880047349.01.T01:CDS | 55.0% | |
| GGTGACCCAATCAAGAGCCA+TGG | + | Chr8:85075198-85075217 | None:intergenic | 55.0% | |
| GTGTGTAGGGTGCACTGCAT+AGG | + | Chr8:85075135-85075154 | None:intergenic | 55.0% | |
| TAGGGTGCACTGCATAGGCA+TGG | + | Chr8:85075130-85075149 | None:intergenic | 55.0% | |
| TGCAGTGCACCCTACACACT+TGG | - | Chr8:85075136-85075155 | MsG0880047349.01.T01:CDS | 55.0% | |
| ! | CTGGACACTGTACTGGTCCT+TGG | - | Chr8:85075512-85075531 | MsG0880047349.01.T01:CDS | 55.0% |
| GGCTCTTGATTGGGTCACCG+AGG | - | Chr8:85075199-85075218 | MsG0880047349.01.T01:CDS | 60.0% | |
| GGTCACCGAGGTTCACGGTA+AGG | - | Chr8:85075211-85075230 | MsG0880047349.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 85074849 | 85075674 | 85074849 | ID=MsG0880047349.01;Name=MsG0880047349.01 |
| Chr8 | mRNA | 85074849 | 85075674 | 85074849 | ID=MsG0880047349.01.T01;Parent=MsG0880047349.01;Name=MsG0880047349.01.T01;_AED=0.49;_eAED=0.53;_QI=0|0|0|1|1|1|2|0|238 |
| Chr8 | exon | 85074989 | 85075674 | 85074989 | ID=MsG0880047349.01.T01:exon:6478;Parent=MsG0880047349.01.T01 |
| Chr8 | exon | 85074849 | 85074879 | 85074849 | ID=MsG0880047349.01.T01:exon:6477;Parent=MsG0880047349.01.T01 |
| Chr8 | CDS | 85074989 | 85075674 | 85074989 | ID=MsG0880047349.01.T01:cds;Parent=MsG0880047349.01.T01 |
| Chr8 | CDS | 85074849 | 85074879 | 85074849 | ID=MsG0880047349.01.T01:cds;Parent=MsG0880047349.01.T01 |
| Gene Sequence |
| Protein sequence |
