AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880047484.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047484.01.T01 MTR_4g128310 95.584 317 6 2 1 310 1 316 0.0 628
MsG0880047484.01.T02 MTR_4g128310 95.425 306 6 2 1 299 12 316 0.0 605
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047484.01.T01 AT5G57500 67.722 316 91 4 3 309 4 317 2.64e-153 432
MsG0880047484.01.T02 AT5G57500 70.370 297 77 4 11 298 23 317 1.68e-153 432

Find 64 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTGACATGTAATGTTTAAA+AGG 0.205102 8:-86992503 None:intergenic
GCTTCCATAAATCTAAAATT+TGG 0.230071 8:+86991981 MsG0880047484.01.T01:CDS
GGACCCTCCACGTGTTTCTT+TGG 0.278399 8:-86992581 None:intergenic
TAAATAGGTTTAATGCAAAA+TGG 0.292393 8:+86992651 MsG0880047484.01.T01:CDS
CCGAAGGAGGATTTATACTA+TGG 0.340654 8:+86992452 MsG0880047484.01.T01:CDS
CATTTCCTCCGTCTAGTATA+CGG 0.365908 8:+86992134 MsG0880047484.01.T01:CDS
TCACCAAACACCTTATCTTC+TGG 0.390933 8:-86992602 None:intergenic
ACTGTTTCGTTGAAAATCTC+TGG 0.393515 8:-86992332 None:intergenic
GATATTGTTGAGTGGATTCA+TGG 0.394735 8:+86992548 MsG0880047484.01.T01:CDS
TCATGACTACAAACAGAAAC+TGG 0.402005 8:-86992695 None:intergenic
GTTTGGTGATTGGATGAGAT+GGG 0.402196 8:+86992616 MsG0880047484.01.T01:CDS
ATTCCTTCAAAGGACCAGTA+TGG 0.406618 8:-86992793 None:intergenic
AAGATAAGGTGTTTGGTGAT+TGG 0.421030 8:+86992606 MsG0880047484.01.T01:CDS
TAGTGGTATGGAGGGTAATC+TGG 0.421093 8:-86992362 None:intergenic
GGAAATGTCGTCGTAGGTAT+TGG 0.424035 8:-86992118 None:intergenic
TAACCATACTGGTCCTTTGA+AGG 0.433399 8:+86992790 MsG0880047484.01.T01:CDS
GGGGTCGTAGAGGATTAAAT+AGG 0.437529 8:+86992636 MsG0880047484.01.T01:CDS
CTACGTGAGGTTAAACAGTT+TGG 0.440226 8:+86992409 MsG0880047484.01.T01:CDS
TTGTTCGTTTGTTGAAATTG+AGG 0.462976 8:-86992042 None:intergenic
AAACATTACATGTCAGGGAT+GGG 0.467261 8:+86992509 MsG0880047484.01.T01:CDS
GGTCCAGAAGATAAGGTGTT+TGG 0.479428 8:+86992599 MsG0880047484.01.T01:CDS
CTGACATGTAATGTTTAAAA+GGG 0.485872 8:-86992502 None:intergenic
TGTCGTCGTAGGTATTGGTC+GGG 0.487255 8:-86992113 None:intergenic
CCATAGTATAAATCCTCCTT+CGG 0.492356 8:-86992452 None:intergenic
CGTGGAGGGTCCAGAAGATA+AGG 0.500488 8:+86992592 MsG0880047484.01.T01:CDS
TGTTTGGTGATTGGATGAGA+TGG 0.507770 8:+86992615 MsG0880047484.01.T01:CDS
TAAACATTACATGTCAGGGA+TGG 0.509130 8:+86992508 MsG0880047484.01.T01:CDS
AACATTACATGTCAGGGATG+GGG 0.512875 8:+86992510 MsG0880047484.01.T01:CDS
TGTTGAAGAATCAAGAGAAG+TGG 0.520516 8:+86992747 MsG0880047484.01.T01:CDS
GTTAAAACCGTTACCGAAGG+AGG 0.529382 8:+86992439 MsG0880047484.01.T01:CDS
GCTTTCATAACGTAGTGGTA+TGG 0.537061 8:-86992374 None:intergenic
CTACAAACAGAAACTGGATC+AGG 0.538009 8:-86992689 None:intergenic
CTAAGCTATATGATACATGT+TGG 0.547955 8:-86992816 None:intergenic
TATAAATCCTCCTTCGGTAA+CGG 0.548830 8:-86992446 None:intergenic
TGTGATACCGTGTGGAAGCA+TGG 0.553514 8:+86992478 MsG0880047484.01.T01:CDS
TACATGTTGGATTCCTTCAA+AGG 0.556547 8:-86992803 None:intergenic
TTCATAACGTAGTGGTATGG+AGG 0.568786 8:-86992371 None:intergenic
ATGTCGTCGTAGGTATTGGT+CGG 0.574850 8:-86992114 None:intergenic
TTCGTTTGTTGAAATTGAGG+TGG 0.579470 8:-86992039 None:intergenic
TTTGGTGATTGGATGAGATG+GGG 0.586718 8:+86992617 MsG0880047484.01.T01:CDS
TGGATGAGATGGGGTCGTAG+AGG 0.587450 8:+86992626 MsG0880047484.01.T01:CDS
TAATATGCAGAGGAAGAAGA+TGG 0.589646 8:-86991958 None:intergenic
ATCGTTAAAACCGTTACCGA+AGG 0.597958 8:+86992436 MsG0880047484.01.T01:CDS
CAAGAGATTCGAATCCTCAT+CGG 0.604375 8:+86992077 MsG0880047484.01.T01:CDS
TGGATCAGGATAATTATACA+TGG 0.630360 8:-86992675 None:intergenic
GTCGTCGTAGGTATTGGTCG+GGG 0.638088 8:-86992112 None:intergenic
TCATAACGTAGTGGTATGGA+GGG 0.642383 8:-86992370 None:intergenic
AAAGGGTCCATGCTTCCACA+CGG 0.645296 8:-86992485 None:intergenic
TAAGTTGGGATATTGTTGAG+TGG 0.646567 8:+86992540 MsG0880047484.01.T01:CDS
TGCACGGAAAACATGAACAA+AGG 0.648841 8:+86992281 MsG0880047484.01.T01:CDS
TTTGTGTACCGTATACTAGA+CGG 0.650769 8:-86992142 None:intergenic
CATCATAATTCTCAACTGCA+CGG 0.654565 8:+86992265 MsG0880047484.01.T01:CDS
GACGGAGGAAATGTCGTCGT+AGG 0.669841 8:-86992124 None:intergenic
TACGGTACACAAAACCCTGA+AGG 0.670631 8:+86992152 MsG0880047484.01.T01:CDS
ATACCAAAGAAACACGTGGA+GGG 0.671115 8:+86992578 MsG0880047484.01.T01:CDS
CATCTGCTTTCATAACGTAG+TGG 0.672252 8:-86992379 None:intergenic
TTATGGAAGCTAATATGCAG+AGG 0.674957 8:-86991968 None:intergenic
GCTTGTGAATGATACTATTG+CGG 0.687103 8:+86992718 MsG0880047484.01.T01:CDS
TGATATACCAAAGAAACACG+TGG 0.712302 8:+86992574 MsG0880047484.01.T01:CDS
CAGATGATGATACCTACGTG+AGG 0.721080 8:+86992396 MsG0880047484.01.T01:CDS
GTGTACCGTATACTAGACGG+AGG 0.721667 8:-86992139 None:intergenic
GGAGTGTGAGAACTCCGATG+AGG 0.728142 8:-86992091 None:intergenic
CAAACTGTTTAACCTCACGT+AGG 0.729711 8:-86992408 None:intergenic
TATACCAAAGAAACACGTGG+AGG 0.751966 8:+86992577 MsG0880047484.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAATAGGTTTAATGCAAAA+TGG + Chr8:86992651-86992670 MsG0880047484.01.T01:CDS 20.0%
!!! CTTAATTTTTTTAACCATAC+TGG + Chr8:86992779-86992798 MsG0880047484.01.T01:CDS 20.0%
! CTGACATGTAATGTTTAAAA+GGG - Chr8:86992505-86992524 None:intergenic 25.0%
!! CTTTTAAACATTACATGTCA+GGG + Chr8:86992504-86992523 MsG0880047484.01.T01:CDS 25.0%
!! GCTTCCATAAATCTAAAATT+TGG + Chr8:86991981-86992000 MsG0880047484.01.T01:CDS 25.0%
!! TCGACCAAATTTTAGATTTA+TGG - Chr8:86991988-86992007 None:intergenic 25.0%
CCTGACATGTAATGTTTAAA+AGG - Chr8:86992506-86992525 None:intergenic 30.0%
CTAAGCTATATGATACATGT+TGG - Chr8:86992819-86992838 None:intergenic 30.0%
TGGATCAGGATAATTATACA+TGG - Chr8:86992678-86992697 None:intergenic 30.0%
! ATTTTCAACGAAACAGTGAA+TGG + Chr8:86992338-86992357 MsG0880047484.01.T01:CDS 30.0%
! CCTTTTAAACATTACATGTC+AGG + Chr8:86992503-86992522 MsG0880047484.01.T01:CDS 30.0%
! TTGTTCGTTTGTTGAAATTG+AGG - Chr8:86992045-86992064 None:intergenic 30.0%
!! ATAAGAGCATGAAACGTTTT+CGG - Chr8:86991929-86991948 None:intergenic 30.0%
AAACATTACATGTCAGGGAT+GGG + Chr8:86992509-86992528 MsG0880047484.01.T01:CDS 35.0%
ACTGTTTCGTTGAAAATCTC+TGG - Chr8:86992335-86992354 None:intergenic 35.0%
CATCATAATTCTCAACTGCA+CGG + Chr8:86992265-86992284 MsG0880047484.01.T01:CDS 35.0%
CCATAGTATAAATCCTCCTT+CGG - Chr8:86992455-86992474 None:intergenic 35.0%
GCTTGTGAATGATACTATTG+CGG + Chr8:86992718-86992737 MsG0880047484.01.T01:CDS 35.0%
TAAACATTACATGTCAGGGA+TGG + Chr8:86992508-86992527 MsG0880047484.01.T01:CDS 35.0%
TAATATGCAGAGGAAGAAGA+TGG - Chr8:86991961-86991980 None:intergenic 35.0%
TACATGTTGGATTCCTTCAA+AGG - Chr8:86992806-86992825 None:intergenic 35.0%
TATAAATCCTCCTTCGGTAA+CGG - Chr8:86992449-86992468 None:intergenic 35.0%
TCATGACTACAAACAGAAAC+TGG - Chr8:86992698-86992717 None:intergenic 35.0%
TGATATACCAAAGAAACACG+TGG + Chr8:86992574-86992593 MsG0880047484.01.T01:CDS 35.0%
TGTTGAAGAATCAAGAGAAG+TGG + Chr8:86992747-86992766 MsG0880047484.01.T01:CDS 35.0%
TTATGGAAGCTAATATGCAG+AGG - Chr8:86991971-86991990 None:intergenic 35.0%
TTTGTGTACCGTATACTAGA+CGG - Chr8:86992145-86992164 None:intergenic 35.0%
! AAGATAAGGTGTTTGGTGAT+TGG + Chr8:86992606-86992625 MsG0880047484.01.T01:CDS 35.0%
! GATATTGTTGAGTGGATTCA+TGG + Chr8:86992548-86992567 MsG0880047484.01.T01:CDS 35.0%
! TAAGTTGGGATATTGTTGAG+TGG + Chr8:86992540-86992559 MsG0880047484.01.T01:CDS 35.0%
! TTCGTTTGTTGAAATTGAGG+TGG - Chr8:86992042-86992061 None:intergenic 35.0%
!!! AAACGTTTTCGGTTTGGTTT+TGG - Chr8:86991918-86991937 None:intergenic 35.0%
!!! AACGTTTTCGGTTTGGTTTT+GGG - Chr8:86991917-86991936 None:intergenic 35.0%
AACATTACATGTCAGGGATG+GGG + Chr8:86992510-86992529 MsG0880047484.01.T01:CDS 40.0%
ATACCAAAGAAACACGTGGA+GGG + Chr8:86992578-86992597 MsG0880047484.01.T01:CDS 40.0%
ATCGTTAAAACCGTTACCGA+AGG + Chr8:86992436-86992455 MsG0880047484.01.T01:CDS 40.0%
ATTCCTTCAAAGGACCAGTA+TGG - Chr8:86992796-86992815 None:intergenic 40.0%
CAAACTGTTTAACCTCACGT+AGG - Chr8:86992411-86992430 None:intergenic 40.0%
CAAGAGATTCGAATCCTCAT+CGG + Chr8:86992077-86992096 MsG0880047484.01.T01:CDS 40.0%
CATCTGCTTTCATAACGTAG+TGG - Chr8:86992382-86992401 None:intergenic 40.0%
CATTTCCTCCGTCTAGTATA+CGG + Chr8:86992134-86992153 MsG0880047484.01.T01:CDS 40.0%
CCGAAGGAGGATTTATACTA+TGG + Chr8:86992452-86992471 MsG0880047484.01.T01:CDS 40.0%
CTACAAACAGAAACTGGATC+AGG - Chr8:86992692-86992711 None:intergenic 40.0%
CTACGTGAGGTTAAACAGTT+TGG + Chr8:86992409-86992428 MsG0880047484.01.T01:CDS 40.0%
GCTTTCATAACGTAGTGGTA+TGG - Chr8:86992377-86992396 None:intergenic 40.0%
TAACCATACTGGTCCTTTGA+AGG + Chr8:86992790-86992809 MsG0880047484.01.T01:CDS 40.0%
TATACCAAAGAAACACGTGG+AGG + Chr8:86992577-86992596 MsG0880047484.01.T01:CDS 40.0%
TCACCAAACACCTTATCTTC+TGG - Chr8:86992605-86992624 None:intergenic 40.0%
TCATAACGTAGTGGTATGGA+GGG - Chr8:86992373-86992392 None:intergenic 40.0%
TGCACGGAAAACATGAACAA+AGG + Chr8:86992281-86992300 MsG0880047484.01.T01:CDS 40.0%
TTCATAACGTAGTGGTATGG+AGG - Chr8:86992374-86992393 None:intergenic 40.0%
! AGCATGAAACGTTTTCGGTT+TGG - Chr8:86991924-86991943 None:intergenic 40.0%
! GTTTGGTGATTGGATGAGAT+GGG + Chr8:86992616-86992635 MsG0880047484.01.T01:CDS 40.0%
! TGTTTGGTGATTGGATGAGA+TGG + Chr8:86992615-86992634 MsG0880047484.01.T01:CDS 40.0%
! TTTGGTGATTGGATGAGATG+GGG + Chr8:86992617-86992636 MsG0880047484.01.T01:CDS 40.0%
!!! ACGTTTTCGGTTTGGTTTTG+GGG - Chr8:86991916-86991935 None:intergenic 40.0%
!!! GATGGGGTTTTTGGTAAGTT+GGG + Chr8:86992526-86992545 MsG0880047484.01.T01:CDS 40.0%
!!! TATGGTTTTGTGATACCGTG+TGG + Chr8:86992470-86992489 MsG0880047484.01.T01:CDS 40.0%
CAGATGATGATACCTACGTG+AGG + Chr8:86992396-86992415 MsG0880047484.01.T01:CDS 45.0%
GGAAATGTCGTCGTAGGTAT+TGG - Chr8:86992121-86992140 None:intergenic 45.0%
GGGGTCGTAGAGGATTAAAT+AGG + Chr8:86992636-86992655 MsG0880047484.01.T01:CDS 45.0%
GTTAAAACCGTTACCGAAGG+AGG + Chr8:86992439-86992458 MsG0880047484.01.T01:CDS 45.0%
TACGGTACACAAAACCCTGA+AGG + Chr8:86992152-86992171 MsG0880047484.01.T01:CDS 45.0%
TAGTGGTATGGAGGGTAATC+TGG - Chr8:86992365-86992384 None:intergenic 45.0%
! GGTCCAGAAGATAAGGTGTT+TGG + Chr8:86992599-86992618 MsG0880047484.01.T01:CDS 45.0%
!! ATGTCGTCGTAGGTATTGGT+CGG - Chr8:86992117-86992136 None:intergenic 45.0%
!!! ACGTCGATTTTTGCACCTTC+AGG - Chr8:86992170-86992189 None:intergenic 45.0%
!!! CGTCGATTTTTGCACCTTCA+GGG - Chr8:86992169-86992188 None:intergenic 45.0%
!!! GGATGGGGTTTTTGGTAAGT+TGG + Chr8:86992525-86992544 MsG0880047484.01.T01:CDS 45.0%
AAAGGGTCCATGCTTCCACA+CGG - Chr8:86992488-86992507 None:intergenic 50.0%
GTGTACCGTATACTAGACGG+AGG - Chr8:86992142-86992161 None:intergenic 50.0%
TGTGATACCGTGTGGAAGCA+TGG + Chr8:86992478-86992497 MsG0880047484.01.T01:CDS 50.0%
! CATGTCAGGGATGGGGTTTT+TGG + Chr8:86992517-86992536 MsG0880047484.01.T01:CDS 50.0%
!! TGTCGTCGTAGGTATTGGTC+GGG - Chr8:86992116-86992135 None:intergenic 50.0%
CGTGGAGGGTCCAGAAGATA+AGG + Chr8:86992592-86992611 MsG0880047484.01.T01:CDS 55.0%
GACGGAGGAAATGTCGTCGT+AGG - Chr8:86992127-86992146 None:intergenic 55.0%
GGACCCTCCACGTGTTTCTT+TGG - Chr8:86992584-86992603 None:intergenic 55.0%
GGAGTGTGAGAACTCCGATG+AGG - Chr8:86992094-86992113 None:intergenic 55.0%
TGGATGAGATGGGGTCGTAG+AGG + Chr8:86992626-86992645 MsG0880047484.01.T01:CDS 55.0%
!! GTCGTCGTAGGTATTGGTCG+GGG - Chr8:86992115-86992134 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr8 gene 86991906 86992838 86991906 ID=MsG0880047484.01;Name=MsG0880047484.01
Chr8 mRNA 86991906 86992838 86991906 ID=MsG0880047484.01.T01;Parent=MsG0880047484.01;Name=MsG0880047484.01.T01;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|310
Chr8 exon 86991906 86992838 86991906 ID=MsG0880047484.01.T01:exon:18524;Parent=MsG0880047484.01.T01
Chr8 CDS 86991906 86992838 86991906 ID=MsG0880047484.01.T01:cds;Parent=MsG0880047484.01.T01
Chr8 mRNA 86991939 86992838 86991939 ID=MsG0880047484.01.T02;Parent=MsG0880047484.01;Name=MsG0880047484.01.T02;_AED=0.02;_eAED=0.02;_QI=0|-1|0|1|-1|1|1|0|299
Chr8 exon 86991939 86992838 86991939 ID=MsG0880047484.01.T02:exon:18525;Parent=MsG0880047484.01.T02
Chr8 CDS 86991939 86992838 86991939 ID=MsG0880047484.01.T02:cds;Parent=MsG0880047484.01.T02
Gene Sequence

>MsG0880047484.01.T01

ATGAAATCCCCAAAACCAAACCGAAAACGTTTCATGCTCTTATGTTTCATATCCATCTTCTTCCTCTGCATATTAGCTTCCATAAATCTAAAATTTGGTCGATGCATACTCTCAAACAACACAACAAACACTTCCACCTCAATTTCAACAAACGAACAACAACAACAACAACAAGAGATTCGAATCCTCATCGGAGTTCTCACACTCCCCGACCAATACCTACGACGACATTTCCTCCGTCTAGTATACGGTACACAAAACCCTGAAGGTGCAAAAATCGACGTGAAGTTCGTGTTCTGTAACATCACAAAAGAAGATCAAAAAACTATAATAGCTTTAGAAATCATGCGTTACAACGACATCATAATTCTCAACTGCACGGAAAACATGAACAAAGGAAAAACATCAACATTCTTCACAAGCTTACCAGAGATTTTCAACGAAACAGTGAATGGACCAGATTACCCTCCATACCACTACGTTATGAAAGCAGATGATGATACCTACGTGAGGTTAAACAGTTTGGTAAAATCGTTAAAACCGTTACCGAAGGAGGATTTATACTATGGTTTTGTGATACCGTGTGGAAGCATGGACCCTTTTAAACATTACATGTCAGGGATGGGGTTTTTGGTAAGTTGGGATATTGTTGAGTGGATTCATGGTTCTGATATACCAAAGAAACACGTGGAGGGTCCAGAAGATAAGGTGTTTGGTGATTGGATGAGATGGGGTCGTAGAGGATTAAATAGGTTTAATGCAAAATGGTCCATGTATAATTATCCTGATCCAGTTTCTGTTTGTAGTCATGAGCTTGTGAATGATACTATTGCGGTTCATTTGTTGAAGAATCAAGAGAAGTGGATTAAGACACTTAATTTTTTTAACCATACTGGTCCTTTGAAGGAATCCAACATGTATCATATAGCTTAG

>MsG0880047484.01.T02

ATGCTCTTATGTTTCATATCCATCTTCTTCCTCTGCATATTAGCTTCCATAAATCTAAAATTTGGTCGATGCATACTCTCAAACAACACAACAAACACTTCCACCTCAATTTCAACAAACGAACAACAACAACAACAACAAGAGATTCGAATCCTCATCGGAGTTCTCACACTCCCCGACCAATACCTACGACGACATTTCCTCCGTCTAGTATACGGTACACAAAACCCTGAAGGTGCAAAAATCGACGTGAAGTTCGTGTTCTGTAACATCACAAAAGAAGATCAAAAAACTATAATAGCTTTAGAAATCATGCGTTACAACGACATCATAATTCTCAACTGCACGGAAAACATGAACAAAGGAAAAACATCAACATTCTTCACAAGCTTACCAGAGATTTTCAACGAAACAGTGAATGGACCAGATTACCCTCCATACCACTACGTTATGAAAGCAGATGATGATACCTACGTGAGGTTAAACAGTTTGGTAAAATCGTTAAAACCGTTACCGAAGGAGGATTTATACTATGGTTTTGTGATACCGTGTGGAAGCATGGACCCTTTTAAACATTACATGTCAGGGATGGGGTTTTTGGTAAGTTGGGATATTGTTGAGTGGATTCATGGTTCTGATATACCAAAGAAACACGTGGAGGGTCCAGAAGATAAGGTGTTTGGTGATTGGATGAGATGGGGTCGTAGAGGATTAAATAGGTTTAATGCAAAATGGTCCATGTATAATTATCCTGATCCAGTTTCTGTTTGTAGTCATGAGCTTGTGAATGATACTATTGCGGTTCATTTGTTGAAGAATCAAGAGAAGTGGATTAAGACACTTAATTTTTTTAACCATACTGGTCCTTTGAAGGAATCCAACATGTATCATATAGCTTAG
Protein sequence

>MsG0880047484.01.T01

MKSPKPNRKRFMLLCFISIFFLCILASINLKFGRCILSNNTTNTSTSISTNEQQQQQQEIRILIGVLTLPDQYLRRHFLRLVYGTQNPEGAKIDVKFVFCNITKEDQKTIIALEIMRYNDIIILNCTENMNKGKTSTFFTSLPEIFNETVNGPDYPPYHYVMKADDDTYVRLNSLVKSLKPLPKEDLYYGFVIPCGSMDPFKHYMSGMGFLVSWDIVEWIHGSDIPKKHVEGPEDKVFGDWMRWGRRGLNRFNAKWSMYNYPDPVSVCSHELVNDTIAVHLLKNQEKWIKTLNFFNHTGPLKESNMYHIA*

>MsG0880047484.01.T02

MLLCFISIFFLCILASINLKFGRCILSNNTTNTSTSISTNEQQQQQQEIRILIGVLTLPDQYLRRHFLRLVYGTQNPEGAKIDVKFVFCNITKEDQKTIIALEIMRYNDIIILNCTENMNKGKTSTFFTSLPEIFNETVNGPDYPPYHYVMKADDDTYVRLNSLVKSLKPLPKEDLYYGFVIPCGSMDPFKHYMSGMGFLVSWDIVEWIHGSDIPKKHVEGPEDKVFGDWMRWGRRGLNRFNAKWSMYNYPDPVSVCSHELVNDTIAVHLLKNQEKWIKTLNFFNHTGPLKESNMYHIA*