Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047490.01.T01 | XP_024637086.1 | 93.694 | 222 | 7 | 1 | 1 | 215 | 12 | 233 | 2.23E-134 | 390 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047490.01.T01 | G7JVH5 | 93.694 | 222 | 7 | 1 | 1 | 215 | 12 | 233 | 1.06e-134 | 390 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0880047484.01 | MsG0880047490.01 | 0.828656 | 7.964150e-55 | 7.966395e-52 |
| MsG0580024999.01 | MsG0880047490.01 | 0.813650 | 2.298868e-51 | 1.509946e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047490.01.T01 | MTR_4g128310 | 93.694 | 222 | 7 | 1 | 1 | 215 | 12 | 233 | 2.70e-138 | 390 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047490.01.T01 | AT5G57500 | 67.442 | 215 | 58 | 4 | 11 | 215 | 23 | 235 | 5.99e-101 | 296 |
Find 48 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTGACATGTAATGTTTAAA+AGG | 0.205102 | 8:-87058519 | None:intergenic |
| GCTTCCATAAATCTAAAATT+TGG | 0.230071 | 8:+87058000 | MsG0880047490.01.T01:CDS |
| CCGAAGGAGGATTTATACTA+TGG | 0.340654 | 8:+87058468 | MsG0880047490.01.T01:CDS |
| CATTTCCTCCGTCTAGTATA+CGG | 0.365908 | 8:+87058150 | MsG0880047490.01.T01:CDS |
| TCACCAAACACCTTATCTTC+TGG | 0.390933 | 8:-87058617 | None:intergenic |
| ACTGTTTCGTTGAAAATCTC+TGG | 0.393515 | 8:-87058348 | None:intergenic |
| GATATTGTTGAGTGGATTCA+TGG | 0.394735 | 8:+87058564 | MsG0880047490.01.T01:CDS |
| AAGATAAGGTGTTTGGTGAT+TGG | 0.421030 | 8:+87058621 | MsG0880047490.01.T01:CDS |
| TAGTGGTATGGAGGGTAATC+TGG | 0.421093 | 8:-87058378 | None:intergenic |
| GGAAATGTCGTCGTAGGTAT+TGG | 0.424035 | 8:-87058134 | None:intergenic |
| CTACGTGAGGTTAAACAGTT+TGG | 0.440226 | 8:+87058425 | MsG0880047490.01.T01:CDS |
| TTGTTCGTTTGTTGAAATTG+AGG | 0.462976 | 8:-87058061 | None:intergenic |
| AAACATTACATGTCAGGGAT+GGG | 0.467261 | 8:+87058525 | MsG0880047490.01.T01:CDS |
| GGTCCAGAAGATAAGGTGTT+TGG | 0.479428 | 8:+87058614 | MsG0880047490.01.T01:CDS |
| CTGACATGTAATGTTTAAAA+GGG | 0.485872 | 8:-87058518 | None:intergenic |
| TGTCGTCGTAGGTATTGGTC+GGG | 0.487255 | 8:-87058129 | None:intergenic |
| CCATAGTATAAATCCTCCTT+CGG | 0.492356 | 8:-87058468 | None:intergenic |
| CGTGGAGGGTCCAGAAGATA+AGG | 0.500488 | 8:+87058607 | MsG0880047490.01.T01:CDS |
| TAAACATTACATGTCAGGGA+TGG | 0.509130 | 8:+87058524 | MsG0880047490.01.T01:CDS |
| AACATTACATGTCAGGGATG+GGG | 0.512875 | 8:+87058526 | MsG0880047490.01.T01:CDS |
| GTTAAAACCGTTACCGAAGG+AGG | 0.529382 | 8:+87058455 | MsG0880047490.01.T01:CDS |
| GCTTTCATAACGTAGTGGTA+TGG | 0.537061 | 8:-87058390 | None:intergenic |
| TATAAATCCTCCTTCGGTAA+CGG | 0.548830 | 8:-87058462 | None:intergenic |
| TGTGATACCGTGTGGAAGCA+TGG | 0.553514 | 8:+87058494 | MsG0880047490.01.T01:CDS |
| TTCATAACGTAGTGGTATGG+AGG | 0.568786 | 8:-87058387 | None:intergenic |
| ATGTCGTCGTAGGTATTGGT+CGG | 0.574850 | 8:-87058130 | None:intergenic |
| TTCGTTTGTTGAAATTGAGG+TGG | 0.579470 | 8:-87058058 | None:intergenic |
| TAATATGCAGAGGAAGAAGA+TGG | 0.589646 | 8:-87057977 | None:intergenic |
| ATCGTTAAAACCGTTACCGA+AGG | 0.597958 | 8:+87058452 | MsG0880047490.01.T01:CDS |
| CAAGAGATTCGAATCCTCAT+CGG | 0.604375 | 8:+87058093 | MsG0880047490.01.T01:CDS |
| GTCGTCGTAGGTATTGGTCG+GGG | 0.638088 | 8:-87058128 | None:intergenic |
| TCATAACGTAGTGGTATGGA+GGG | 0.642383 | 8:-87058386 | None:intergenic |
| AAAGGGTCCATGCTTCCACA+CGG | 0.645296 | 8:-87058501 | None:intergenic |
| TAAGTTGGGATATTGTTGAG+TGG | 0.646567 | 8:+87058556 | MsG0880047490.01.T01:CDS |
| TGCACGGAAAACATGAACAA+AGG | 0.648841 | 8:+87058297 | MsG0880047490.01.T01:CDS |
| TTTGTGTACCGTATACTAGA+CGG | 0.650769 | 8:-87058158 | None:intergenic |
| CATCATAATTCTCAACTGCA+CGG | 0.654565 | 8:+87058281 | MsG0880047490.01.T01:CDS |
| GACGGAGGAAATGTCGTCGT+AGG | 0.669841 | 8:-87058140 | None:intergenic |
| TACGGTACACAAAACCCTGA+AGG | 0.670631 | 8:+87058168 | MsG0880047490.01.T01:CDS |
| CATCTGCTTTCATAACGTAG+TGG | 0.672252 | 8:-87058395 | None:intergenic |
| TTATGGAAGCTAATATGCAG+AGG | 0.674957 | 8:-87057987 | None:intergenic |
| TATACAAAGAAACACGTGGA+GGG | 0.682941 | 8:+87058593 | MsG0880047490.01.T01:CDS |
| CTGATATACAAAGAAACACG+TGG | 0.696450 | 8:+87058589 | MsG0880047490.01.T01:CDS |
| CAGATGATGATACCTACGTG+AGG | 0.721080 | 8:+87058412 | MsG0880047490.01.T01:CDS |
| GTGTACCGTATACTAGACGG+AGG | 0.721667 | 8:-87058155 | None:intergenic |
| GGAGTGTGAGAACTCCGATG+AGG | 0.728142 | 8:-87058107 | None:intergenic |
| CAAACTGTTTAACCTCACGT+AGG | 0.729711 | 8:-87058424 | None:intergenic |
| ATATACAAAGAAACACGTGG+AGG | 0.730370 | 8:+87058592 | MsG0880047490.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CTGACATGTAATGTTTAAAA+GGG | - | Chr8:87058521-87058540 | None:intergenic | 25.0% |
| !! | CTTTTAAACATTACATGTCA+GGG | + | Chr8:87058520-87058539 | MsG0880047490.01.T01:CDS | 25.0% |
| !! | GCTTCCATAAATCTAAAATT+TGG | + | Chr8:87058000-87058019 | MsG0880047490.01.T01:CDS | 25.0% |
| !! | TCGACCAAATTTTAGATTTA+TGG | - | Chr8:87058007-87058026 | None:intergenic | 25.0% |
| CCTGACATGTAATGTTTAAA+AGG | - | Chr8:87058522-87058541 | None:intergenic | 30.0% | |
| ! | ATTTTCAACGAAACAGTGAA+TGG | + | Chr8:87058354-87058373 | MsG0880047490.01.T01:CDS | 30.0% |
| ! | CCTTTTAAACATTACATGTC+AGG | + | Chr8:87058519-87058538 | MsG0880047490.01.T01:CDS | 30.0% |
| ! | TTGTTCGTTTGTTGAAATTG+AGG | - | Chr8:87058064-87058083 | None:intergenic | 30.0% |
| AAACATTACATGTCAGGGAT+GGG | + | Chr8:87058525-87058544 | MsG0880047490.01.T01:CDS | 35.0% | |
| ACTGTTTCGTTGAAAATCTC+TGG | - | Chr8:87058351-87058370 | None:intergenic | 35.0% | |
| ATATACAAAGAAACACGTGG+AGG | + | Chr8:87058592-87058611 | MsG0880047490.01.T01:CDS | 35.0% | |
| CATCATAATTCTCAACTGCA+CGG | + | Chr8:87058281-87058300 | MsG0880047490.01.T01:CDS | 35.0% | |
| CCATAGTATAAATCCTCCTT+CGG | - | Chr8:87058471-87058490 | None:intergenic | 35.0% | |
| CTGATATACAAAGAAACACG+TGG | + | Chr8:87058589-87058608 | MsG0880047490.01.T01:CDS | 35.0% | |
| TAAACATTACATGTCAGGGA+TGG | + | Chr8:87058524-87058543 | MsG0880047490.01.T01:CDS | 35.0% | |
| TAATATGCAGAGGAAGAAGA+TGG | - | Chr8:87057980-87057999 | None:intergenic | 35.0% | |
| TATAAATCCTCCTTCGGTAA+CGG | - | Chr8:87058465-87058484 | None:intergenic | 35.0% | |
| TATACAAAGAAACACGTGGA+GGG | + | Chr8:87058593-87058612 | MsG0880047490.01.T01:CDS | 35.0% | |
| TTATGGAAGCTAATATGCAG+AGG | - | Chr8:87057990-87058009 | None:intergenic | 35.0% | |
| TTTGTGTACCGTATACTAGA+CGG | - | Chr8:87058161-87058180 | None:intergenic | 35.0% | |
| ! | AAGATAAGGTGTTTGGTGAT+TGG | + | Chr8:87058621-87058640 | MsG0880047490.01.T01:CDS | 35.0% |
| ! | GATATTGTTGAGTGGATTCA+TGG | + | Chr8:87058564-87058583 | MsG0880047490.01.T01:CDS | 35.0% |
| ! | TAAGTTGGGATATTGTTGAG+TGG | + | Chr8:87058556-87058575 | MsG0880047490.01.T01:CDS | 35.0% |
| ! | TTCGTTTGTTGAAATTGAGG+TGG | - | Chr8:87058061-87058080 | None:intergenic | 35.0% |
| AACATTACATGTCAGGGATG+GGG | + | Chr8:87058526-87058545 | MsG0880047490.01.T01:CDS | 40.0% | |
| ATCGTTAAAACCGTTACCGA+AGG | + | Chr8:87058452-87058471 | MsG0880047490.01.T01:CDS | 40.0% | |
| CAAACTGTTTAACCTCACGT+AGG | - | Chr8:87058427-87058446 | None:intergenic | 40.0% | |
| CAAGAGATTCGAATCCTCAT+CGG | + | Chr8:87058093-87058112 | MsG0880047490.01.T01:CDS | 40.0% | |
| CATCTGCTTTCATAACGTAG+TGG | - | Chr8:87058398-87058417 | None:intergenic | 40.0% | |
| CATTTCCTCCGTCTAGTATA+CGG | + | Chr8:87058150-87058169 | MsG0880047490.01.T01:CDS | 40.0% | |
| CCGAAGGAGGATTTATACTA+TGG | + | Chr8:87058468-87058487 | MsG0880047490.01.T01:CDS | 40.0% | |
| CTACGTGAGGTTAAACAGTT+TGG | + | Chr8:87058425-87058444 | MsG0880047490.01.T01:CDS | 40.0% | |
| GCTTTCATAACGTAGTGGTA+TGG | - | Chr8:87058393-87058412 | None:intergenic | 40.0% | |
| TCACCAAACACCTTATCTTC+TGG | - | Chr8:87058620-87058639 | None:intergenic | 40.0% | |
| TCATAACGTAGTGGTATGGA+GGG | - | Chr8:87058389-87058408 | None:intergenic | 40.0% | |
| TGCACGGAAAACATGAACAA+AGG | + | Chr8:87058297-87058316 | MsG0880047490.01.T01:CDS | 40.0% | |
| TTCATAACGTAGTGGTATGG+AGG | - | Chr8:87058390-87058409 | None:intergenic | 40.0% | |
| ! | ACGTCAATTTTTGCACCTTC+AGG | - | Chr8:87058186-87058205 | None:intergenic | 40.0% |
| ! | CGTCAATTTTTGCACCTTCA+GGG | - | Chr8:87058185-87058204 | None:intergenic | 40.0% |
| !!! | GATGGGGTTTTTGGTAAGTT+GGG | + | Chr8:87058542-87058561 | MsG0880047490.01.T01:CDS | 40.0% |
| !!! | TATGGTTTTGTGATACCGTG+TGG | + | Chr8:87058486-87058505 | MsG0880047490.01.T01:CDS | 40.0% |
| CAGATGATGATACCTACGTG+AGG | + | Chr8:87058412-87058431 | MsG0880047490.01.T01:CDS | 45.0% | |
| GGAAATGTCGTCGTAGGTAT+TGG | - | Chr8:87058137-87058156 | None:intergenic | 45.0% | |
| GTTAAAACCGTTACCGAAGG+AGG | + | Chr8:87058455-87058474 | MsG0880047490.01.T01:CDS | 45.0% | |
| TACGGTACACAAAACCCTGA+AGG | + | Chr8:87058168-87058187 | MsG0880047490.01.T01:CDS | 45.0% | |
| TAGTGGTATGGAGGGTAATC+TGG | - | Chr8:87058381-87058400 | None:intergenic | 45.0% | |
| ! | GGTCCAGAAGATAAGGTGTT+TGG | + | Chr8:87058614-87058633 | MsG0880047490.01.T01:CDS | 45.0% |
| !! | ATGTCGTCGTAGGTATTGGT+CGG | - | Chr8:87058133-87058152 | None:intergenic | 45.0% |
| !!! | GGATGGGGTTTTTGGTAAGT+TGG | + | Chr8:87058541-87058560 | MsG0880047490.01.T01:CDS | 45.0% |
| AAAGGGTCCATGCTTCCACA+CGG | - | Chr8:87058504-87058523 | None:intergenic | 50.0% | |
| GTGTACCGTATACTAGACGG+AGG | - | Chr8:87058158-87058177 | None:intergenic | 50.0% | |
| TGTGATACCGTGTGGAAGCA+TGG | + | Chr8:87058494-87058513 | MsG0880047490.01.T01:CDS | 50.0% | |
| ! | CATGTCAGGGATGGGGTTTT+TGG | + | Chr8:87058533-87058552 | MsG0880047490.01.T01:CDS | 50.0% |
| !! | TGTCGTCGTAGGTATTGGTC+GGG | - | Chr8:87058132-87058151 | None:intergenic | 50.0% |
| CGTGGAGGGTCCAGAAGATA+AGG | + | Chr8:87058607-87058626 | MsG0880047490.01.T01:CDS | 55.0% | |
| GACGGAGGAAATGTCGTCGT+AGG | - | Chr8:87058143-87058162 | None:intergenic | 55.0% | |
| GGAGTGTGAGAACTCCGATG+AGG | - | Chr8:87058110-87058129 | None:intergenic | 55.0% | |
| !! | GTCGTCGTAGGTATTGGTCG+GGG | - | Chr8:87058131-87058150 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 87057958 | 87058647 | 87057958 | ID=MsG0880047490.01;Name=MsG0880047490.01 |
| Chr8 | mRNA | 87057958 | 87058647 | 87057958 | ID=MsG0880047490.01.T01;Parent=MsG0880047490.01;Name=MsG0880047490.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|229 |
| Chr8 | exon | 87057958 | 87058647 | 87057958 | ID=MsG0880047490.01.T01:exon:17984;Parent=MsG0880047490.01.T01 |
| Chr8 | CDS | 87057958 | 87058647 | 87057958 | ID=MsG0880047490.01.T01:cds;Parent=MsG0880047490.01.T01 |
| Gene Sequence |
| Protein sequence |
