Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047729.01.T01 | XP_003610594.2 | 73.488 | 215 | 21 | 3 | 1 | 179 | 309 | 523 | 4.31E-93 | 292 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047729.01.T01 | Q8GYX3 | 44.624 | 186 | 82 | 5 | 1 | 179 | 289 | 460 | 2.39E-35 | 132 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047729.01.T01 | A0A396IHM9 | 73.488 | 215 | 21 | 3 | 1 | 179 | 309 | 523 | 2.06e-93 | 292 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0880047728.01 | MsG0880047729.01 | 0.876376 | 1.466359e-68 | 7.092392e-65 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047729.01.T01 | MTR_4g133890 | 73.488 | 215 | 21 | 3 | 1 | 179 | 301 | 515 | 6.46e-97 | 291 |
| MsG0880047729.01.T01 | MTR_4g133890 | 73.488 | 215 | 21 | 3 | 1 | 179 | 309 | 523 | 9.87e-97 | 291 |
| MsG0880047729.01.T01 | MTR_2g026590 | 47.926 | 217 | 58 | 5 | 1 | 175 | 296 | 499 | 8.60e-39 | 139 |
| MsG0880047729.01.T01 | MTR_8g021120 | 38.835 | 206 | 97 | 9 | 1 | 179 | 336 | 539 | 7.59e-19 | 83.6 |
| MsG0880047729.01.T01 | MTR_8g105350 | 37.441 | 211 | 95 | 8 | 1 | 179 | 340 | 545 | 1.09e-17 | 80.1 |
| MsG0880047729.01.T01 | MTR_1g032520 | 34.300 | 207 | 107 | 6 | 1 | 179 | 335 | 540 | 1.39e-15 | 74.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047729.01.T01 | AT4G17220 | 44.624 | 186 | 82 | 5 | 1 | 179 | 289 | 460 | 2.44e-36 | 132 |
| MsG0880047729.01.T01 | AT1G24764 | 33.971 | 209 | 107 | 7 | 1 | 179 | 204 | 411 | 3.51e-17 | 79.0 |
| MsG0880047729.01.T01 | AT1G24764 | 35.211 | 213 | 99 | 8 | 1 | 179 | 357 | 564 | 4.11e-17 | 78.6 |
| MsG0880047729.01.T01 | AT1G14840 | 33.810 | 210 | 102 | 9 | 1 | 179 | 332 | 535 | 4.26e-17 | 78.6 |
| MsG0880047729.01.T01 | AT1G14840 | 33.810 | 210 | 102 | 9 | 1 | 179 | 317 | 520 | 4.48e-17 | 78.6 |
| MsG0880047729.01.T01 | AT1G24764 | 33.971 | 209 | 107 | 7 | 1 | 179 | 416 | 623 | 4.57e-17 | 78.6 |
| MsG0880047729.01.T01 | AT2G01750 | 33.023 | 215 | 96 | 7 | 1 | 179 | 356 | 558 | 1.07e-16 | 77.4 |
| MsG0880047729.01.T01 | AT2G01750 | 33.023 | 215 | 96 | 7 | 1 | 179 | 356 | 558 | 1.10e-16 | 77.4 |
| MsG0880047729.01.T01 | AT1G68060 | 31.401 | 207 | 112 | 5 | 1 | 179 | 349 | 553 | 7.20e-16 | 75.1 |
Find 26 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTTGCAAATTACTGTCTT+TGG | 0.222434 | 8:-90096678 | None:intergenic |
| CCACTATCAGCAACTTTACT+TGG | 0.264053 | 8:-90096449 | None:intergenic |
| AGCTAAAGATGAAGAAATTA+TGG | 0.313202 | 8:+90096697 | MsG0880047729.01.T01:CDS |
| ATAAGTTCCCCAACAGAAAA+TGG | 0.325320 | 8:+90096257 | MsG0880047729.01.T01:CDS |
| AGTCATTTGGTTTCTTGCTT+TGG | 0.339262 | 8:-90096574 | None:intergenic |
| TGCAGTGACGATTTGGTGTC+AGG | 0.351949 | 8:+90096599 | MsG0880047729.01.T01:CDS |
| CTTCACTTTCAGCATTGAAC+TGG | 0.389403 | 8:-90096517 | None:intergenic |
| CAGAAAACATGTTGAAGAAA+AGG | 0.410590 | 8:+90096424 | MsG0880047729.01.T01:intron |
| TCGTCACTGCACGAGTCATT+TGG | 0.432134 | 8:-90096587 | None:intergenic |
| TGAAAAGGAAAATGAGATAC+AGG | 0.451771 | 8:+90096472 | MsG0880047729.01.T01:CDS |
| AGTTGCTGATAGTGGTGAAA+AGG | 0.454137 | 8:+90096457 | MsG0880047729.01.T01:CDS |
| TGACTCGTGCAGTGACGATT+TGG | 0.459803 | 8:+90096592 | MsG0880047729.01.T01:CDS |
| CTTCCAATCGCAGAAGCTCT+TGG | 0.464587 | 8:-90096308 | None:intergenic |
| CTTCATCTGTGATTCTGCCT+TGG | 0.465848 | 8:-90096076 | None:intergenic |
| CAACCAAGAGCTTCTGCGAT+TGG | 0.480093 | 8:+90096305 | MsG0880047729.01.T01:CDS |
| CCAAGTAAAGTTGCTGATAG+TGG | 0.491571 | 8:+90096449 | MsG0880047729.01.T01:CDS |
| ATACATAGCTAGTGAAAGTC+GGG | 0.520886 | 8:-90096737 | None:intergenic |
| CAAGGCAGAATCACAGATGA+AGG | 0.551311 | 8:+90096077 | MsG0880047729.01.T01:CDS |
| AAAGAGAGGTCATTAAATTG+AGG | 0.563049 | 8:+90096642 | MsG0880047729.01.T01:CDS |
| GATACATAGCTAGTGAAAGT+CGG | 0.593463 | 8:-90096738 | None:intergenic |
| AAATGTAGACAGAGATGCAA+AGG | 0.598221 | 8:+90096019 | None:intergenic |
| GTATGATAGGCTGCAAAGAG+AGG | 0.608475 | 8:+90096628 | MsG0880047729.01.T01:CDS |
| TATATCAGAGAGAACAGCCA+AGG | 0.654453 | 8:+90096059 | MsG0880047729.01.T01:CDS |
| AAAGGAAAATGAGATACAGG+TGG | 0.667770 | 8:+90096475 | MsG0880047729.01.T01:CDS |
| AGAAGCTCTTGGTTGTGATG+TGG | 0.668929 | 8:-90096297 | None:intergenic |
| AGTTCCCCAACAGAAAATGG+TGG | 0.700541 | 8:+90096260 | MsG0880047729.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GAAGTATATTTCATATTAAT+AGG | - | Chr8:90096402-90096421 | None:intergenic | 15.0% |
| ! | AGCTAAAGATGAAGAAATTA+TGG | + | Chr8:90096697-90096716 | MsG0880047729.01.T01:CDS | 25.0% |
| ! | ATATTTGTCTGTTGAAACAA+AGG | - | Chr8:90096335-90096354 | None:intergenic | 25.0% |
| ! | TATTTGTCTGTTGAAACAAA+GGG | - | Chr8:90096334-90096353 | None:intergenic | 25.0% |
| !! | AATTGATTTAGATGCTTACA+CGG | - | Chr8:90096127-90096146 | None:intergenic | 25.0% |
| !!! | TCAATTTTCAACGTGTTATT+TGG | + | Chr8:90096141-90096160 | MsG0880047729.01.T01:intron | 25.0% |
| AAAAGTGAGACTAAAAACAC+TGG | + | Chr8:90096184-90096203 | MsG0880047729.01.T01:CDS | 30.0% | |
| AAAGAGAGGTCATTAAATTG+AGG | + | Chr8:90096642-90096661 | MsG0880047729.01.T01:CDS | 30.0% | |
| CAGAAAACATGTTGAAGAAA+AGG | + | Chr8:90096424-90096443 | MsG0880047729.01.T01:intron | 30.0% | |
| TAAAAAACAACACACACTGT+TGG | + | Chr8:90096354-90096373 | MsG0880047729.01.T01:intron | 30.0% | |
| TGAAAAGGAAAATGAGATAC+AGG | + | Chr8:90096472-90096491 | MsG0880047729.01.T01:CDS | 30.0% | |
| !! | TGTTATTTGGTGTTTACTTC+AGG | + | Chr8:90096154-90096173 | MsG0880047729.01.T01:intron | 30.0% |
| !!! | TGTCAGGTTTTTTGTATGAT+AGG | + | Chr8:90096615-90096634 | MsG0880047729.01.T01:CDS | 30.0% |
| AAAGGAAAATGAGATACAGG+TGG | + | Chr8:90096475-90096494 | MsG0880047729.01.T01:CDS | 35.0% | |
| AGCTTGCAAATTACTGTCTT+TGG | - | Chr8:90096681-90096700 | None:intergenic | 35.0% | |
| ATAAGTTCCCCAACAGAAAA+TGG | + | Chr8:90096257-90096276 | MsG0880047729.01.T01:CDS | 35.0% | |
| ! | AGTCATTTGGTTTCTTGCTT+TGG | - | Chr8:90096577-90096596 | None:intergenic | 35.0% |
| !!! | TGTGATGTGGATCTCTTTTT+TGG | - | Chr8:90096287-90096306 | None:intergenic | 35.0% |
| AAAACAACACACACTGTTGG+AGG | + | Chr8:90096357-90096376 | MsG0880047729.01.T01:intron | 40.0% | |
| AAACAACACACACTGTTGGA+GGG | + | Chr8:90096358-90096377 | MsG0880047729.01.T01:intron | 40.0% | |
| AGACTAAAAACACTGGAGGA+AGG | + | Chr8:90096191-90096210 | MsG0880047729.01.T01:CDS | 40.0% | |
| AGTGAGACTAAAAACACTGG+AGG | + | Chr8:90096187-90096206 | MsG0880047729.01.T01:CDS | 40.0% | |
| CCAAGTAAAGTTGCTGATAG+TGG | + | Chr8:90096449-90096468 | MsG0880047729.01.T01:CDS | 40.0% | |
| CCACTATCAGCAACTTTACT+TGG | - | Chr8:90096452-90096471 | None:intergenic | 40.0% | |
| TATATCAGAGAGAACAGCCA+AGG | + | Chr8:90096059-90096078 | MsG0880047729.01.T01:CDS | 40.0% | |
| TGTTGGAGGGAATTCAAAAC+AGG | + | Chr8:90096371-90096390 | MsG0880047729.01.T01:intron | 40.0% | |
| ! | CTTCACTTTCAGCATTGAAC+TGG | - | Chr8:90096520-90096539 | None:intergenic | 40.0% |
| ! | TTGGACCACCATTTTCTGTT+GGG | - | Chr8:90096268-90096287 | None:intergenic | 40.0% |
| ! | TTTGGACCACCATTTTCTGT+TGG | - | Chr8:90096269-90096288 | None:intergenic | 40.0% |
| !! | AGTTGCTGATAGTGGTGAAA+AGG | + | Chr8:90096457-90096476 | MsG0880047729.01.T01:CDS | 40.0% |
| AGAAGCTCTTGGTTGTGATG+TGG | - | Chr8:90096300-90096319 | None:intergenic | 45.0% | |
| AGTTCCCCAACAGAAAATGG+TGG | + | Chr8:90096260-90096279 | MsG0880047729.01.T01:CDS | 45.0% | |
| CAAGGCAGAATCACAGATGA+AGG | + | Chr8:90096077-90096096 | MsG0880047729.01.T01:CDS | 45.0% | |
| CTTCATCTGTGATTCTGCCT+TGG | - | Chr8:90096079-90096098 | None:intergenic | 45.0% | |
| GTATGATAGGCTGCAAAGAG+AGG | + | Chr8:90096628-90096647 | MsG0880047729.01.T01:CDS | 45.0% | |
| ! | TGGACCACCATTTTCTGTTG+GGG | - | Chr8:90096267-90096286 | None:intergenic | 45.0% |
| CAACCAAGAGCTTCTGCGAT+TGG | + | Chr8:90096305-90096324 | MsG0880047729.01.T01:CDS | 50.0% | |
| CTTCCAATCGCAGAAGCTCT+TGG | - | Chr8:90096311-90096330 | None:intergenic | 50.0% | |
| TCGTCACTGCACGAGTCATT+TGG | - | Chr8:90096590-90096609 | None:intergenic | 50.0% | |
| TGACTCGTGCAGTGACGATT+TGG | + | Chr8:90096592-90096611 | MsG0880047729.01.T01:CDS | 50.0% | |
| !! | TGCAGTGACGATTTGGTGTC+AGG | + | Chr8:90096599-90096618 | MsG0880047729.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 90096033 | 90096751 | 90096033 | ID=MsG0880047729.01;Name=MsG0880047729.01 |
| Chr8 | mRNA | 90096033 | 90096751 | 90096033 | ID=MsG0880047729.01.T01;Parent=MsG0880047729.01;Name=MsG0880047729.01.T01;_AED=0.23;_eAED=0.23;_QI=0|0|0|1|1|1|3|0|183 |
| Chr8 | exon | 90096033 | 90096098 | 90096033 | ID=MsG0880047729.01.T01:exon:2606;Parent=MsG0880047729.01.T01 |
| Chr8 | exon | 90096176 | 90096336 | 90096176 | ID=MsG0880047729.01.T01:exon:2607;Parent=MsG0880047729.01.T01 |
| Chr8 | exon | 90096427 | 90096751 | 90096427 | ID=MsG0880047729.01.T01:exon:2608;Parent=MsG0880047729.01.T01 |
| Chr8 | CDS | 90096033 | 90096098 | 90096033 | ID=MsG0880047729.01.T01:cds;Parent=MsG0880047729.01.T01 |
| Chr8 | CDS | 90096176 | 90096336 | 90096176 | ID=MsG0880047729.01.T01:cds;Parent=MsG0880047729.01.T01 |
| Chr8 | CDS | 90096427 | 90096751 | 90096427 | ID=MsG0880047729.01.T01:cds;Parent=MsG0880047729.01.T01 |
| Gene Sequence |
| Protein sequence |
