AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880047728.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047728.01.T01 MTR_4g133890 78.068 383 49 3 160 510 190 569 0.0 561
MsG0880047728.01.T01 MTR_4g133890 77.604 384 51 3 160 511 182 562 0.0 561
MsG0880047728.01.T01 MTR_2g026590 53.713 579 151 10 17 511 4 549 2.05e-173 500
MsG0880047728.01.T01 MTR_1g032520 40.511 548 214 12 25 491 51 567 3.88e-102 319
MsG0880047728.01.T01 MTR_8g021120 38.779 557 209 13 25 491 52 566 1.10e-91 292
MsG0880047728.01.T01 MTR_8g105350 38.198 555 217 15 25 491 56 572 1.17e-88 284
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047728.01.T01 AT4G17220 47.094 499 175 9 71 502 22 498 1.12e-121 367
MsG0880047728.01.T01 AT2G01750 39.891 549 213 12 25 491 72 585 2.89e-102 320
MsG0880047728.01.T01 AT2G01750 39.891 549 213 12 25 491 72 585 3.06e-102 320
MsG0880047728.01.T01 AT1G14840 39.068 558 206 13 25 491 48 562 5.94e-102 318
MsG0880047728.01.T01 AT1G24764 38.849 556 214 12 25 491 73 591 4.06e-95 302
MsG0880047728.01.T01 AT1G24764 38.849 556 214 12 25 491 132 650 2.75e-94 301
MsG0880047728.01.T01 AT1G68060 38.339 566 232 15 25 506 65 597 1.70e-92 295
MsG0880047728.01.T01 AT1G24764 42.152 446 161 9 135 491 1 438 2.33e-91 287
MsG0880047728.01.T01 AT1G14840 36.738 558 204 14 25 491 48 547 1.42e-88 283

Find 95 sgRNAs with CRISPR-Local

Find 213 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCGAGATTTCTCTAAAATTT+TGG 0.168689 8:-90065971 None:intergenic
ATATTTGTCTGTTGAAATAA+AGG 0.235016 8:-90067165 None:intergenic
ATTTCCCTTTCCCTCACTTT+TGG 0.259668 8:-90064147 None:intergenic
AGCTTGCAAACTACTGTCTT+TGG 0.271121 8:-90067529 None:intergenic
TAATTAAAATACCTCTTCTA+TGG 0.278017 8:-90064963 None:intergenic
TTTCAACTTCAAGAATCTTT+TGG 0.283060 8:-90065996 None:intergenic
TGGACCACCACTTGCTGTTC+GGG 0.284359 8:-90067097 None:intergenic
AAAGCTTAGCCAAACTATTC+AGG 0.293484 8:+90066017 MsG0880047728.01.T01:CDS
GAACAGAATCGTCATCCTTT+TGG 0.300614 8:-90065507 None:intergenic
GTATCAGCCCACTAACCTTT+TGG 0.306246 8:-90068107 None:intergenic
GCAATTCAGAAACTTGTCTT+TGG 0.311316 8:-90066095 None:intergenic
CCTCAAGTTCCTGAATAGTT+TGG 0.312176 8:-90066026 None:intergenic
AGCTAAAGATGAAGAAATTA+TGG 0.313202 8:+90067548 MsG0880047728.01.T01:CDS
TTCACCATTTGCAATTCTAT+TGG 0.325874 8:-90066259 None:intergenic
AGTCATTTGGTTTCTTGCTT+TGG 0.339262 8:-90067425 None:intergenic
TTGGACCACCACTTGCTGTT+CGG 0.348999 8:-90067098 None:intergenic
TGCAGTGACGATTTGGTGTC+AGG 0.351949 8:+90067450 MsG0880047728.01.T01:CDS
GTTCGGGAACTTATGATGTT+AGG 0.366965 8:-90067081 None:intergenic
CAACCAAGAGCATCTGCTAT+TGG 0.377463 8:+90067138 MsG0880047728.01.T01:CDS
GAGAAATGAAGTCGGTAAAC+TGG 0.378705 8:+90065242 MsG0880047728.01.T01:CDS
CCGCAGAAGCAACACTTAGA+AGG 0.382381 8:+90065473 MsG0880047728.01.T01:CDS
TCTTCTAGCCATTGCTTGAC+AGG 0.383618 8:-90066318 None:intergenic
AAAACAACACAAACACTGTT+GGG 0.389131 8:+90067190 MsG0880047728.01.T01:CDS
CTTCCACTCTACTACTTCCA+TGG 0.404073 8:-90067993 None:intergenic
TATTTGTCTGTTGAAATAAA+GGG 0.417797 8:-90067164 None:intergenic
GCTAGCAAGGGTAAAAGTTT+CGG 0.430627 8:+90066236 MsG0880047728.01.T01:CDS
TCGTCACTGCACGAGTCATT+TGG 0.432134 8:-90067438 None:intergenic
GGGGCAATGGCGTTATCAAT+TGG 0.440234 8:-90065529 None:intergenic
TATGCCGAACTAGTATTGAA+TGG 0.442930 8:+90064596 MsG0880047728.01.T01:intron
ATAGGGTCTGAGAGGACAAC+TGG 0.454257 8:-90064204 None:intergenic
GTGAGGTGTGAAAAGCAATA+TGG 0.458438 8:+90064171 MsG0880047728.01.T01:CDS
TGACTCGTGCAGTGACGATT+TGG 0.459803 8:+90067443 MsG0880047728.01.T01:CDS
CTCTACTACTTCCATGGACT+TGG 0.472683 8:-90067987 None:intergenic
AAGCTGAGATTACAATGTAT+AGG 0.477680 8:+90065557 MsG0880047728.01.T01:CDS
CTTCAGCTGTGATTCTGCCT+TGG 0.478370 8:-90066910 None:intergenic
CACTAGAGAGGGAGCTAGCA+AGG 0.481581 8:+90066223 MsG0880047728.01.T01:CDS
AAAAGTGAGGGAAAGGGAAA+TGG 0.487123 8:+90064149 MsG0880047728.01.T01:CDS
CTTCCAATAGCAGATGCTCT+TGG 0.493116 8:-90067141 None:intergenic
AGAGGATCGTCTTAAGCAAA+AGG 0.497139 8:+90065284 MsG0880047728.01.T01:CDS
CCTCTCAGACCCTATGGTGT+TGG 0.497422 8:+90064212 MsG0880047728.01.T01:CDS
AGATGCAGCTGCTAGAGAAA+AGG 0.499613 8:+90068027 MsG0880047728.01.T01:CDS
AGACTCCTCCAAAAGGTTAG+TGG 0.500956 8:+90068099 MsG0880047728.01.T01:CDS
CGATTAATCTCCAACACCAT+AGG 0.511794 8:-90064222 None:intergenic
AGTTGTCCTCTCAGACCCTA+TGG 0.511922 8:+90064206 MsG0880047728.01.T01:CDS
GACTGAAGCTCTGAAGGATA+AGG 0.515576 8:+90064940 MsG0880047728.01.T01:CDS
CAATGCCAAAAGTGAGGGAA+AGG 0.521517 8:+90064142 None:intergenic
TCATGACTGAGACAATGATC+TGG 0.525253 8:-90064645 None:intergenic
CTCAGCTTGAAGAGGGGCAA+TGG 0.525958 8:-90065542 None:intergenic
GACTCCTCCAAAAGGTTAGT+GGG 0.527997 8:+90068100 MsG0880047728.01.T01:CDS
TTGTAATCTCAGCTTGAAGA+GGG 0.530115 8:-90065549 None:intergenic
CCAAACTATTCAGGAACTTG+AGG 0.536454 8:+90066026 MsG0880047728.01.T01:CDS
AAGGGTGCATGCAAACCAAA+AGG 0.538099 8:+90065492 MsG0880047728.01.T01:CDS
CCTTCTAAGTGTTGCTTCTG+CGG 0.541428 8:-90065473 None:intergenic
CGCAGAAGCAACACTTAGAA+GGG 0.542238 8:+90065474 MsG0880047728.01.T01:CDS
GAAACACAGACTCCTCCAAA+AGG 0.543913 8:+90068092 MsG0880047728.01.T01:CDS
TCGGCCAATAGAATTGCAAA+TGG 0.544219 8:+90066255 MsG0880047728.01.T01:CDS
TGATGCACTGACCAAGTCCA+TGG 0.550345 8:+90067976 MsG0880047728.01.T01:CDS
AAAGAGAGGTCATTAAATTG+AGG 0.563049 8:+90067493 MsG0880047728.01.T01:CDS
AAACAACACAAACACTGTTG+GGG 0.563286 8:+90067191 MsG0880047728.01.T01:CDS
GATTAATCTCCAACACCATA+GGG 0.563826 8:-90064221 None:intergenic
AGAAGAGAGAAGGATTATGC+AGG 0.567503 8:+90066335 MsG0880047728.01.T01:CDS
AAGGTCGACTGAAGCTCTGA+AGG 0.573607 8:+90064934 MsG0880047728.01.T01:CDS
CCTCCCAATGCCAAAAGTGA+GGG 0.575640 8:+90064137 None:intergenic
AGCAATGGCTAGAAGAGAGA+AGG 0.577331 8:+90066325 MsG0880047728.01.T01:CDS
CAAGGCAGAATCACAGCTGA+AGG 0.578308 8:+90066911 MsG0880047728.01.T01:CDS
AATGCCAAAAGTGAGGGAAA+GGG 0.592729 8:+90064143 None:intergenic
TCCTCCCAATGCCAAAAGTG+AGG 0.596299 8:+90064136 None:intergenic
AAATGTAGACAGAGATGCAA+AGG 0.598221 8:+90066853 MsG0880047728.01.T01:intron
GAGGAAGTCATCTTAGCTAA+CGG 0.600860 8:+90066045 MsG0880047728.01.T01:CDS
GATTACAATGTATAGGAATG+AGG 0.604046 8:+90065564 MsG0880047728.01.T01:CDS
AGGTCATGCCTGTCAAGCAA+TGG 0.604618 8:+90066310 MsG0880047728.01.T01:CDS
ATGAGAAAAGGACACTAGAG+AGG 0.607096 8:+90066211 MsG0880047728.01.T01:CDS
GTATGATAGGCTGCAAAGAG+AGG 0.608475 8:+90067479 MsG0880047728.01.T01:CDS
ACTAGAGAGGGAGCTAGCAA+GGG 0.615439 8:+90066224 MsG0880047728.01.T01:CDS
TAAAATGAAGTATACTTCTG+CGG 0.618529 8:+90067239 MsG0880047728.01.T01:CDS
CGAGAGACTTAGAGTTACAG+AGG 0.619933 8:+90065266 MsG0880047728.01.T01:CDS
AGAGTGAAATCAAAGCACTA+AGG 0.626155 8:+90064915 MsG0880047728.01.T01:CDS
GAAGGATAAGGCCATAGAAG+AGG 0.631116 8:+90064952 MsG0880047728.01.T01:CDS
ATAAGTTCCCGAACAGCAAG+TGG 0.632187 8:+90067090 MsG0880047728.01.T01:CDS
TGGTGAAGCTAATGAGTGGA+AGG 0.633476 8:+90066275 MsG0880047728.01.T01:CDS
AGTCCATGGAAGTAGTAGAG+TGG 0.634747 8:+90067990 MsG0880047728.01.T01:CDS
TGAGGGAAAGGGAAATGGTG+AGG 0.637716 8:+90064154 MsG0880047728.01.T01:CDS
AACAACACAAACACTGTTGG+GGG 0.647416 8:+90067192 MsG0880047728.01.T01:CDS
ATTGTAATCTCAGCTTGAAG+AGG 0.647918 8:-90065550 None:intergenic
AGACGAAAAGAAAAGTGCAA+TGG 0.651443 8:+90065438 MsG0880047728.01.T01:CDS
TGAGAAAAGGACACTAGAGA+GGG 0.660251 8:+90066212 MsG0880047728.01.T01:CDS
AGTTCCCGAACAGCAAGTGG+TGG 0.662519 8:+90067093 MsG0880047728.01.T01:CDS
GTGTGAAAAGCAATATGGAG+AGG 0.669978 8:+90064176 MsG0880047728.01.T01:CDS
TATATCAGAGAGAACAGCCA+AGG 0.670465 8:+90066893 MsG0880047728.01.T01:CDS
CCAACACCATAGGGTCTGAG+AGG 0.675725 8:-90064212 None:intergenic
TTTCAGCTGAGAAATGAAGT+CGG 0.679362 8:+90065234 MsG0880047728.01.T01:intron
GTGGAAGGATGAAAATGACA+AGG 0.684047 8:+90066290 MsG0880047728.01.T01:CDS
TGTAATCTCAGCTTGAAGAG+GGG 0.692586 8:-90065548 None:intergenic
CAAATGGTGAAGCTAATGAG+TGG 0.695992 8:+90066271 MsG0880047728.01.T01:CDS
GGGTCTGAGAGGACAACTGG+AGG 0.711528 8:-90064201 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATTATACTACAAATTAAT+AGG - Chr8:90065087-90065106 None:intergenic 10.0%
!! AAATAAAACTGTAGTATTTA+AGG - Chr8:90066424-90066443 None:intergenic 15.0%
!! AATGATTACATAAAGTTATA+AGG - Chr8:90064861-90064880 None:intergenic 15.0%
!! ATATACTGAAATAAATCTTA+AGG + Chr8:90066484-90066503 MsG0880047728.01.T01:intron 15.0%
!! TATACTGAAATAAATCTTAA+GGG + Chr8:90066485-90066504 MsG0880047728.01.T01:intron 15.0%
!!! GTTTTATTTTGTTACTTATA+AGG + Chr8:90066435-90066454 MsG0880047728.01.T01:intron 15.0%
!!! TACTTGTATTTTATTAAAGT+AGG + Chr8:90065322-90065341 MsG0880047728.01.T01:intron 15.0%
!!! TTTAAAACTCTAATAAAGAT+TGG - Chr8:90064714-90064733 None:intergenic 15.0%
!! AGTTTGTAGAAATTTCATTT+AGG - Chr8:90066820-90066839 None:intergenic 20.0%
!! ATAACTTTATGTAATCATTG+TGG + Chr8:90064862-90064881 MsG0880047728.01.T01:intron 20.0%
!! ATATTTGTCTGTTGAAATAA+AGG - Chr8:90067168-90067187 None:intergenic 20.0%
!! GTTTGTAGAAATTTCATTTA+GGG - Chr8:90066819-90066838 None:intergenic 20.0%
!! TAATTAAAATACCTCTTCTA+TGG - Chr8:90064966-90064985 None:intergenic 20.0%
!! TATTTGTCTGTTGAAATAAA+GGG - Chr8:90067167-90067186 None:intergenic 20.0%
!!! ACAATTGTTTTTGATTTCTT+CGG - Chr8:90067387-90067406 None:intergenic 20.0%
!!! ATATTGTTATTGTAGTTTTC+AGG - Chr8:90066468-90066487 None:intergenic 20.0%
!!! GAAGTATACTTCATTTTAAT+AGG - Chr8:90067237-90067256 None:intergenic 20.0%
!!! GTTGTTTTTATAAGTTATCT+TGG + Chr8:90066669-90066688 MsG0880047728.01.T01:intron 20.0%
!!! TTTATGAAATGTGTGTTTTA+TGG - Chr8:90067866-90067885 None:intergenic 20.0%
! AAAAGTGAGACTAAAAACAA+TGG + Chr8:90067017-90067036 MsG0880047728.01.T01:CDS 25.0%
! AACATGCATGACATAATTAA+TGG - Chr8:90065042-90065061 None:intergenic 25.0%
! AACTAGGATTTCCATATTTA+AGG + Chr8:90066537-90066556 MsG0880047728.01.T01:intron 25.0%
! AATCTATCTATACAAACACA+TGG + Chr8:90064986-90065005 MsG0880047728.01.T01:intron 25.0%
! AGCTAAAGATGAAGAAATTA+TGG + Chr8:90067548-90067567 MsG0880047728.01.T01:CDS 25.0%
! ATATCCATTCAATACTAGTT+CGG - Chr8:90064603-90064622 None:intergenic 25.0%
! ATGTCATCTATATAAAGTGT+CGG - Chr8:90067616-90067635 None:intergenic 25.0%
! CCATATTAGACTGAATAATA+CGG - Chr8:90065194-90065213 None:intergenic 25.0%
! TAAAATGAAGTATACTTCTG+CGG + Chr8:90067239-90067258 MsG0880047728.01.T01:CDS 25.0%
! TCAAACATGATAATTCAAAC+AGG + Chr8:90066793-90066812 MsG0880047728.01.T01:intron 25.0%
! TTGTGTTCAACTTATACTTA+TGG - Chr8:90064804-90064823 None:intergenic 25.0%
!! AATTGATTTAGATGCTTACA+TGG - Chr8:90066961-90066980 None:intergenic 25.0%
!! ATAAACTTCAAAGGTTTTCA+TGG - Chr8:90066384-90066403 None:intergenic 25.0%
!! GGTTTTGCATATAAATTTTC+AGG + Chr8:90066188-90066207 MsG0880047728.01.T01:intron 25.0%
!! TGATATATTGACTGTTTGTT+TGG + Chr8:90066143-90066162 MsG0880047728.01.T01:intron 25.0%
!! TTCTGCATAAGAATAGATTT+TGG - Chr8:90065397-90065416 None:intergenic 25.0%
!! TTTCAACTTCAAGAATCTTT+TGG - Chr8:90065999-90066018 None:intergenic 25.0%
!!! AATAATACGGTTCTAAATTG+AGG - Chr8:90065181-90065200 None:intergenic 25.0%
!!! CTGGTCAATACTTTTTTTTT+GGG - Chr8:90064629-90064648 None:intergenic 25.0%
!!! TAAATTTTCAGGATGAGAAA+AGG + Chr8:90066199-90066218 MsG0880047728.01.T01:intron 25.0%
!!! TCTGGTCAATACTTTTTTTT+TGG - Chr8:90064630-90064649 None:intergenic 25.0%
AAAAACAACACAAACACTGT+TGG + Chr8:90067189-90067208 MsG0880047728.01.T01:CDS 30.0%
AAAACAACACAAACACTGTT+GGG + Chr8:90067190-90067209 MsG0880047728.01.T01:CDS 30.0%
AAAGAGAGGTCATTAAATTG+AGG + Chr8:90067493-90067512 MsG0880047728.01.T01:CDS 30.0%
AAATACTACCATTTCCAACA+AGG + Chr8:90065598-90065617 MsG0880047728.01.T01:intron 30.0%
AAATGAACTATTCAAGTCAG+TGG + Chr8:90064823-90064842 MsG0880047728.01.T01:intron 30.0%
AAATGATTCCTTGTTGGAAA+TGG - Chr8:90065609-90065628 None:intergenic 30.0%
AAATTATTACCAGTGTCTTC+CGG + Chr8:90067722-90067741 MsG0880047728.01.T01:intron 30.0%
AACATGTGAAGAAAAGGATA+TGG + Chr8:90067264-90067283 MsG0880047728.01.T01:intron 30.0%
AAGCTGAGATTACAATGTAT+AGG + Chr8:90065557-90065576 MsG0880047728.01.T01:CDS 30.0%
AAGTCTTTCATATCACATAC+GGG - Chr8:90064764-90064783 None:intergenic 30.0%
AATTTACAACTACCACAAAC+AGG + Chr8:90065942-90065961 MsG0880047728.01.T01:intron 30.0%
ACATAATTAATGGTCCCTAA+TGG - Chr8:90065032-90065051 None:intergenic 30.0%
ATAAGTAAAGTTGCTGATAG+TGG + Chr8:90067293-90067312 MsG0880047728.01.T01:intron 30.0%
ATAATCAGTGTGAGATTGTT+TGG - Chr8:90066759-90066778 None:intergenic 30.0%
ATGTTTCAAAGTCTACAAGT+AGG + Chr8:90067789-90067808 MsG0880047728.01.T01:intron 30.0%
CAGGATGATATAAACTTCAA+AGG - Chr8:90066393-90066412 None:intergenic 30.0%
CCGTATTATTCAGTCTAATA+TGG + Chr8:90065191-90065210 MsG0880047728.01.T01:intron 30.0%
GAAGTCTTTCATATCACATA+CGG - Chr8:90064765-90064784 None:intergenic 30.0%
GATTACAATGTATAGGAATG+AGG + Chr8:90065564-90065583 MsG0880047728.01.T01:CDS 30.0%
TAATCAGTGTGAGATTGTTT+GGG - Chr8:90066758-90066777 None:intergenic 30.0%
TGAAAAGGAAAATGAGATAC+AGG + Chr8:90067316-90067335 MsG0880047728.01.T01:intron 30.0%
TGTTTGTGGTAGTTGTAAAT+TGG - Chr8:90065943-90065962 None:intergenic 30.0%
TTATACTTATGGTGACTTCT+AGG - Chr8:90064793-90064812 None:intergenic 30.0%
TTCACCATTTGCAATTCTAT+TGG - Chr8:90066262-90066281 None:intergenic 30.0%
TTGATTGGACTCCTTAAATA+TGG - Chr8:90066551-90066570 None:intergenic 30.0%
TTTGCAAGGATAGATCAAAA+TGG + Chr8:90065626-90065645 MsG0880047728.01.T01:intron 30.0%
! AGGGTAAGTTTAAGTTTAAG+AGG + Chr8:90066504-90066523 MsG0880047728.01.T01:intron 30.0%
! AGTTTGATTTAGTTTCCATC+TGG + Chr8:90064306-90064325 MsG0880047728.01.T01:intron 30.0%
! ATAAGCAGCTTTATCCATTA+GGG + Chr8:90065015-90065034 MsG0880047728.01.T01:intron 30.0%
! GAGAGAAAAACTTGCATTTT+AGG - Chr8:90065371-90065390 None:intergenic 30.0%
! GGGTAAGTTTAAGTTTAAGA+GGG + Chr8:90066505-90066524 MsG0880047728.01.T01:intron 30.0%
! TTTGTTTGGTTGATCTTTTG+TGG + Chr8:90066157-90066176 MsG0880047728.01.T01:intron 30.0%
!!! GCGAGATTTCTCTAAAATTT+TGG - Chr8:90065974-90065993 None:intergenic 30.0%
!!! TGTCAGGTTTTTTGTATGAT+AGG + Chr8:90067466-90067485 MsG0880047728.01.T01:CDS 30.0%
!!! TGTGATGTAGATCTCTTTTT+TGG - Chr8:90067120-90067139 None:intergenic 30.0%
!!! TTTTTTCTGTTTCCATCATG+AGG + Chr8:90064276-90064295 MsG0880047728.01.T01:intron 30.0%
AAACAACACAAACACTGTTG+GGG + Chr8:90067191-90067210 MsG0880047728.01.T01:CDS 35.0%
AAACTCAATACTCCTCATGA+TGG - Chr8:90064291-90064310 None:intergenic 35.0%
AAAGCTTAGCCAAACTATTC+AGG + Chr8:90066017-90066036 MsG0880047728.01.T01:CDS 35.0%
AAATGTAGACAGAGATGCAA+AGG + Chr8:90066853-90066872 MsG0880047728.01.T01:intron 35.0%
AGACTAAAAACAATGGAGGA+AGG + Chr8:90067024-90067043 MsG0880047728.01.T01:CDS 35.0%
AGTCTTTCATATCACATACG+GGG - Chr8:90064763-90064782 None:intergenic 35.0%
AGTGAGACTAAAAACAATGG+AGG + Chr8:90067020-90067039 MsG0880047728.01.T01:CDS 35.0%
ATTGAATGTAACCATGTGTC+AGG - Chr8:90067687-90067706 None:intergenic 35.0%
ATTGTAATCTCAGCTTGAAG+AGG - Chr8:90065553-90065572 None:intergenic 35.0%
CATTGTGGCAGAAAAAGTTA+AGG + Chr8:90064877-90064896 MsG0880047728.01.T01:intron 35.0%
CTTCATTTCTCAGCTGAAAA+TGG - Chr8:90065233-90065252 None:intergenic 35.0%
GATTAATCTCCAACACCATA+GGG - Chr8:90064224-90064243 None:intergenic 35.0%
GCAATTCAGAAACTTGTCTT+TGG - Chr8:90066098-90066117 None:intergenic 35.0%
TACTAGTTCGGCATAAAACA+AGG - Chr8:90064591-90064610 None:intergenic 35.0%
TTCATAGATAACAGCACTTC+AGG + Chr8:90065690-90065709 MsG0880047728.01.T01:intron 35.0%
TTGTAATCTCAGCTTGAAGA+GGG - Chr8:90065552-90065571 None:intergenic 35.0%
TTTCAGCTGAGAAATGAAGT+CGG + Chr8:90065234-90065253 MsG0880047728.01.T01:intron 35.0%
! AAGCAAAAGGTACACTGATT+TGG + Chr8:90065297-90065316 MsG0880047728.01.T01:intron 35.0%
! AGACGAAAAGAAAAGTGCAA+TGG + Chr8:90065438-90065457 MsG0880047728.01.T01:CDS 35.0%
! AGAGTGAAATCAAAGCACTA+AGG + Chr8:90064915-90064934 MsG0880047728.01.T01:CDS 35.0%
! AGTATTTAAGGCATGAAAGC+AGG - Chr8:90066412-90066431 None:intergenic 35.0%
! AGTCATTTGGTTTCTTGCTT+TGG - Chr8:90067428-90067447 None:intergenic 35.0%
! CAACTTCAAGAATCTTTTGG+CGG - Chr8:90065996-90066015 None:intergenic 35.0%
! CATAAGCAGCTTTATCCATT+AGG + Chr8:90065014-90065033 MsG0880047728.01.T01:intron 35.0%
! GACCAAAATTTAGAACGATG+CGG - Chr8:90065135-90065154 None:intergenic 35.0%
! GAGTTCGTTACTGTAGTTTT+AGG + Chr8:90067839-90067858 MsG0880047728.01.T01:intron 35.0%
! GGTTCTAGCAATCTTCAATA+TGG - Chr8:90065350-90065369 None:intergenic 35.0%
! TATGCCGAACTAGTATTGAA+TGG + Chr8:90064596-90064615 MsG0880047728.01.T01:intron 35.0%
! TGATCTTTTGTGGTAACTTG+TGG + Chr8:90066167-90066186 MsG0880047728.01.T01:intron 35.0%
! TGTTGGGGGAAATTTAAAAC+TGG + Chr8:90067206-90067225 MsG0880047728.01.T01:CDS 35.0%
!! GTGTTATTTGTGTTCACTTC+AGG + Chr8:90066987-90067006 MsG0880047728.01.T01:intron 35.0%
!! TTATGGTGACTTCTAGGATA+AGG - Chr8:90064787-90064806 None:intergenic 35.0%
!! TTCCGCATCGTTCTAAATTT+TGG + Chr8:90065130-90065149 MsG0880047728.01.T01:intron 35.0%
AAAAGTGAGGGAAAGGGAAA+TGG + Chr8:90064149-90064168 MsG0880047728.01.T01:CDS 40.0%
AACAACACAAACACTGTTGG+GGG + Chr8:90067192-90067211 MsG0880047728.01.T01:CDS 40.0%
AGAAGAGAGAAGGATTATGC+AGG + Chr8:90066335-90066354 MsG0880047728.01.T01:CDS 40.0%
AGAGGATCGTCTTAAGCAAA+AGG + Chr8:90065284-90065303 MsG0880047728.01.T01:CDS 40.0%
AGCTTGCAAACTACTGTCTT+TGG - Chr8:90067532-90067551 None:intergenic 40.0%
ATACATAGCTAGTGAAAGCG+GGG - Chr8:90067591-90067610 None:intergenic 40.0%
ATGAGAAAAGGACACTAGAG+AGG + Chr8:90066211-90066230 MsG0880047728.01.T01:CDS 40.0%
CAAATGGTGAAGCTAATGAG+TGG + Chr8:90066271-90066290 MsG0880047728.01.T01:CDS 40.0%
CATCTCTGTCTACATTTGCA+AGG - Chr8:90066850-90066869 None:intergenic 40.0%
CATTTCTCAGCTGAAAATGG+TGG - Chr8:90065230-90065249 None:intergenic 40.0%
CCAAACTATTCAGGAACTTG+AGG + Chr8:90066026-90066045 MsG0880047728.01.T01:CDS 40.0%
CCAACAAGGAATCATTTGCA+AGG + Chr8:90065612-90065631 MsG0880047728.01.T01:intron 40.0%
CCTCAAGTTCCTGAATAGTT+TGG - Chr8:90066029-90066048 None:intergenic 40.0%
CCTTGCAAATGATTCCTTGT+TGG - Chr8:90065615-90065634 None:intergenic 40.0%
CGATACATAGCTAGTGAAAG+CGG - Chr8:90067593-90067612 None:intergenic 40.0%
CGATTAATCTCCAACACCAT+AGG - Chr8:90064225-90064244 None:intergenic 40.0%
CTGCTCACAAGTAGAAAGTT+TGG - Chr8:90064686-90064705 None:intergenic 40.0%
GAAACGCAAAAAATCTTGCC+TGG - Chr8:90065867-90065886 None:intergenic 40.0%
GAGAAATGAAGTCGGTAAAC+TGG + Chr8:90065242-90065261 MsG0880047728.01.T01:CDS 40.0%
GAGACAGATTGATATCTACC+AGG + Chr8:90065846-90065865 MsG0880047728.01.T01:intron 40.0%
GAGGAAGTCATCTTAGCTAA+CGG + Chr8:90066045-90066064 MsG0880047728.01.T01:CDS 40.0%
GATACATAGCTAGTGAAAGC+GGG - Chr8:90067592-90067611 None:intergenic 40.0%
GCGGAAAACATGTGAAGAAA+AGG + Chr8:90067258-90067277 MsG0880047728.01.T01:intron 40.0%
GCTAGCAAGGGTAAAAGTTT+CGG + Chr8:90066236-90066255 MsG0880047728.01.T01:CDS 40.0%
GTCAGTATCAGAAACCAACA+TGG + Chr8:90067660-90067679 MsG0880047728.01.T01:intron 40.0%
GTCTTTCATATCACATACGG+GGG - Chr8:90064762-90064781 None:intergenic 40.0%
GTGAGGTGTGAAAAGCAATA+TGG + Chr8:90064171-90064190 MsG0880047728.01.T01:CDS 40.0%
GTGGAAGGATGAAAATGACA+AGG + Chr8:90066290-90066309 MsG0880047728.01.T01:CDS 40.0%
GTGTGAAAAGCAATATGGAG+AGG + Chr8:90064176-90064195 MsG0880047728.01.T01:CDS 40.0%
GTTCGGGAACTTATGATGTT+AGG - Chr8:90067084-90067103 None:intergenic 40.0%
GTTGAACAGCTTCTCTTGAT+TGG - Chr8:90066566-90066585 None:intergenic 40.0%
TATATCAGAGAGAACAGCCA+AGG + Chr8:90066893-90066912 MsG0880047728.01.T01:CDS 40.0%
TCATGACTGAGACAATGATC+TGG - Chr8:90064648-90064667 None:intergenic 40.0%
TCGGCCAATAGAATTGCAAA+TGG + Chr8:90066255-90066274 MsG0880047728.01.T01:CDS 40.0%
TGAGAAAAGGACACTAGAGA+GGG + Chr8:90066212-90066231 MsG0880047728.01.T01:CDS 40.0%
TGAGAATCAAACTGACCAGA+TGG - Chr8:90064324-90064343 None:intergenic 40.0%
TGTAATCTCAGCTTGAAGAG+GGG - Chr8:90065551-90065570 None:intergenic 40.0%
TTACAACTACCACAAACAGG+AGG + Chr8:90065945-90065964 MsG0880047728.01.T01:intron 40.0%
TTTGCAAAGTGCACTAGAGA+AGG + Chr8:90065780-90065799 MsG0880047728.01.T01:intron 40.0%
! AAGAGGGTGTAATGCAAACT+AGG + Chr8:90066521-90066540 MsG0880047728.01.T01:intron 40.0%
! ATTTCCCTTTCCCTCACTTT+TGG - Chr8:90064150-90064169 None:intergenic 40.0%
! CTTCACTTTCAGCATTGAAC+TGG - Chr8:90067364-90067383 None:intergenic 40.0%
! GAACAGAATCGTCATCCTTT+TGG - Chr8:90065510-90065529 None:intergenic 40.0%
!! ACGGTTCTAAATTGAGGTTG+CGG - Chr8:90065175-90065194 None:intergenic 40.0%
!! AGTTGCTGATAGTGGTGAAA+AGG + Chr8:90067301-90067320 MsG0880047728.01.T01:intron 40.0%
AACACGACACTGACACTAAC+CGG - Chr8:90067744-90067763 None:intergenic 45.0%
AGACTCCTCCAAAAGGTTAG+TGG + Chr8:90068099-90068118 MsG0880047728.01.T01:CDS 45.0%
AGATGCAGCTGCTAGAGAAA+AGG + Chr8:90068027-90068046 MsG0880047728.01.T01:CDS 45.0%
AGCAATGGCTAGAAGAGAGA+AGG + Chr8:90066325-90066344 MsG0880047728.01.T01:CDS 45.0%
AGTCCATGGAAGTAGTAGAG+TGG + Chr8:90067990-90068009 MsG0880047728.01.T01:CDS 45.0%
ATAAGTTCCCGAACAGCAAG+TGG + Chr8:90067090-90067109 MsG0880047728.01.T01:CDS 45.0%
CGAGAGACTTAGAGTTACAG+AGG + Chr8:90065266-90065285 MsG0880047728.01.T01:CDS 45.0%
CGCAGAAGCAACACTTAGAA+GGG + Chr8:90065474-90065493 MsG0880047728.01.T01:CDS 45.0%
CTCTACTACTTCCATGGACT+TGG - Chr8:90067990-90068009 None:intergenic 45.0%
CTTCCAATAGCAGATGCTCT+TGG - Chr8:90067144-90067163 None:intergenic 45.0%
CTTCCACTCTACTACTTCCA+TGG - Chr8:90067996-90068015 None:intergenic 45.0%
GAAACACAGACTCCTCCAAA+AGG + Chr8:90068092-90068111 MsG0880047728.01.T01:CDS 45.0%
GAAGGATAAGGCCATAGAAG+AGG + Chr8:90064952-90064971 MsG0880047728.01.T01:CDS 45.0%
GACTCCTCCAAAAGGTTAGT+GGG + Chr8:90068100-90068119 MsG0880047728.01.T01:CDS 45.0%
GTATGATAGGCTGCAAAGAG+AGG + Chr8:90067479-90067498 MsG0880047728.01.T01:CDS 45.0%
TCTTCTAGCCATTGCTTGAC+AGG - Chr8:90066321-90066340 None:intergenic 45.0%
TGGTGAAGCTAATGAGTGGA+AGG + Chr8:90066275-90066294 MsG0880047728.01.T01:CDS 45.0%
TTGGTCCACAACTGCAATTG+TGG + Chr8:90065149-90065168 MsG0880047728.01.T01:intron 45.0%
! AAGCATTGCTAGATGCAGAG+AGG + Chr8:90065755-90065774 MsG0880047728.01.T01:intron 45.0%
! AAGGGTGCATGCAAACCAAA+AGG + Chr8:90065492-90065511 MsG0880047728.01.T01:CDS 45.0%
! TTTTGGTCTCCTCCTGTTTG+TGG - Chr8:90065957-90065976 None:intergenic 45.0%
!! CAACCAAGAGCATCTGCTAT+TGG + Chr8:90067138-90067157 MsG0880047728.01.T01:CDS 45.0%
!! CCTTCTAAGTGTTGCTTCTG+CGG - Chr8:90065476-90065495 None:intergenic 45.0%
!! GACTGAAGCTCTGAAGGATA+AGG + Chr8:90064940-90064959 MsG0880047728.01.T01:CDS 45.0%
!! TTATATTCATTATAATTTAT+AGG - Chr8:90064396-90064415 None:intergenic 5.0%
!! TTATTATAAAAAAGAAAAAA+GGG - Chr8:90065889-90065908 None:intergenic 5.0%
!! TTTATTATAAAAAAGAAAAA+AGG - Chr8:90065890-90065909 None:intergenic 5.0%
!!! TCTTTTTTATAATAAAAAAT+TGG + Chr8:90065894-90065913 MsG0880047728.01.T01:intron 5.0%
ACACGTGCCAGACATTGACA+CGG - Chr8:90067642-90067661 None:intergenic 50.0%
ACATGTCCCGTGTCAATGTC+TGG + Chr8:90067632-90067651 MsG0880047728.01.T01:intron 50.0%
ACCAACATGGTCCTGACACA+TGG + Chr8:90067673-90067692 MsG0880047728.01.T01:intron 50.0%
ACCATGTGTCAGGACCATGT+TGG - Chr8:90067677-90067696 None:intergenic 50.0%
ACTAGAGAGGGAGCTAGCAA+GGG + Chr8:90066224-90066243 MsG0880047728.01.T01:CDS 50.0%
AGGTCATGCCTGTCAAGCAA+TGG + Chr8:90066310-90066329 MsG0880047728.01.T01:CDS 50.0%
AGTTGTCCTCTCAGACCCTA+TGG + Chr8:90064206-90064225 MsG0880047728.01.T01:CDS 50.0%
ATAGGGTCTGAGAGGACAAC+TGG - Chr8:90064207-90064226 None:intergenic 50.0%
CAAGGCAGAATCACAGCTGA+AGG + Chr8:90066911-90066930 MsG0880047728.01.T01:CDS 50.0%
CCGCAGAAGCAACACTTAGA+AGG + Chr8:90065473-90065492 MsG0880047728.01.T01:CDS 50.0%
CTTCAGCTGTGATTCTGCCT+TGG - Chr8:90066913-90066932 None:intergenic 50.0%
GGGGCAATGGCGTTATCAAT+TGG - Chr8:90065532-90065551 None:intergenic 50.0%
GGTTAGTGTCAGTGTCGTGT+TGG + Chr8:90067743-90067762 MsG0880047728.01.T01:intron 50.0%
TCGTCACTGCACGAGTCATT+TGG - Chr8:90067441-90067460 None:intergenic 50.0%
TGACACTAACCGGAAGACAC+TGG - Chr8:90067734-90067753 None:intergenic 50.0%
TGACTCGTGCAGTGACGATT+TGG + Chr8:90067443-90067462 MsG0880047728.01.T01:CDS 50.0%
TGAGGGAAAGGGAAATGGTG+AGG + Chr8:90064154-90064173 MsG0880047728.01.T01:CDS 50.0%
! AAGGTCGACTGAAGCTCTGA+AGG + Chr8:90064934-90064953 MsG0880047728.01.T01:CDS 50.0%
! TCAGCCCACTAACCTTTTGG+AGG - Chr8:90068107-90068126 None:intergenic 50.0%
! TGATGCACTGACCAAGTCCA+TGG + Chr8:90067976-90067995 MsG0880047728.01.T01:CDS 50.0%
!! TGCAGTGACGATTTGGTGTC+AGG + Chr8:90067450-90067469 MsG0880047728.01.T01:CDS 50.0%
!! TTGGACCACCACTTGCTGTT+CGG - Chr8:90067101-90067120 None:intergenic 50.0%
AGTTCCCGAACAGCAAGTGG+TGG + Chr8:90067093-90067112 MsG0880047728.01.T01:CDS 55.0%
CACGTGCCAGACATTGACAC+GGG - Chr8:90067641-90067660 None:intergenic 55.0%
CACTAGAGAGGGAGCTAGCA+AGG + Chr8:90066223-90066242 MsG0880047728.01.T01:CDS 55.0%
CCAACACCATAGGGTCTGAG+AGG - Chr8:90064215-90064234 None:intergenic 55.0%
CCTCTCAGACCCTATGGTGT+TGG + Chr8:90064212-90064231 MsG0880047728.01.T01:CDS 55.0%
CTCAGCTTGAAGAGGGGCAA+TGG - Chr8:90065545-90065564 None:intergenic 55.0%
TGCGGCCACAATTGCAGTTG+TGG - Chr8:90065157-90065176 None:intergenic 55.0%
!! TGGACCACCACTTGCTGTTC+GGG - Chr8:90067100-90067119 None:intergenic 55.0%
GGGTCTGAGAGGACAACTGG+AGG - Chr8:90064204-90064223 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr8 gene 90064144 90068126 90064144 ID=MsG0880047728.01;Name=MsG0880047728.01
Chr8 mRNA 90064144 90068126 90064144 ID=MsG0880047728.01.T01;Parent=MsG0880047728.01;Name=MsG0880047728.01.T01;_AED=0.33;_eAED=0.37;_QI=0|0.6|0.45|1|0.6|0.72|11|0|512
Chr8 exon 90064144 90064283 90064144 ID=MsG0880047728.01.T01:exon:2595;Parent=MsG0880047728.01.T01
Chr8 exon 90064609 90064676 90064609 ID=MsG0880047728.01.T01:exon:2596;Parent=MsG0880047728.01.T01
Chr8 exon 90064888 90064973 90064888 ID=MsG0880047728.01.T01:exon:2597;Parent=MsG0880047728.01.T01
Chr8 exon 90065240 90065305 90065240 ID=MsG0880047728.01.T01:exon:2598;Parent=MsG0880047728.01.T01
Chr8 exon 90065415 90065585 90065415 ID=MsG0880047728.01.T01:exon:2599;Parent=MsG0880047728.01.T01
Chr8 exon 90065964 90066119 90065964 ID=MsG0880047728.01.T01:exon:2600;Parent=MsG0880047728.01.T01
Chr8 exon 90066210 90066356 90066210 ID=MsG0880047728.01.T01:exon:2601;Parent=MsG0880047728.01.T01
Chr8 exon 90066861 90066932 90066861 ID=MsG0880047728.01.T01:exon:2602;Parent=MsG0880047728.01.T01
Chr8 exon 90067009 90067268 90067009 ID=MsG0880047728.01.T01:exon:2603;Parent=MsG0880047728.01.T01
Chr8 exon 90067365 90067569 90067365 ID=MsG0880047728.01.T01:exon:2604;Parent=MsG0880047728.01.T01
Chr8 exon 90067959 90068126 90067959 ID=MsG0880047728.01.T01:exon:2605;Parent=MsG0880047728.01.T01
Chr8 CDS 90064144 90064283 90064144 ID=MsG0880047728.01.T01:cds;Parent=MsG0880047728.01.T01
Chr8 CDS 90064609 90064676 90064609 ID=MsG0880047728.01.T01:cds;Parent=MsG0880047728.01.T01
Chr8 CDS 90064888 90064973 90064888 ID=MsG0880047728.01.T01:cds;Parent=MsG0880047728.01.T01
Chr8 CDS 90065240 90065305 90065240 ID=MsG0880047728.01.T01:cds;Parent=MsG0880047728.01.T01
Chr8 CDS 90065415 90065585 90065415 ID=MsG0880047728.01.T01:cds;Parent=MsG0880047728.01.T01
Chr8 CDS 90065964 90066119 90065964 ID=MsG0880047728.01.T01:cds;Parent=MsG0880047728.01.T01
Chr8 CDS 90066210 90066356 90066210 ID=MsG0880047728.01.T01:cds;Parent=MsG0880047728.01.T01
Chr8 CDS 90066861 90066932 90066861 ID=MsG0880047728.01.T01:cds;Parent=MsG0880047728.01.T01
Chr8 CDS 90067009 90067268 90067009 ID=MsG0880047728.01.T01:cds;Parent=MsG0880047728.01.T01
Chr8 CDS 90067365 90067569 90067365 ID=MsG0880047728.01.T01:cds;Parent=MsG0880047728.01.T01
Chr8 CDS 90067959 90068126 90067959 ID=MsG0880047728.01.T01:cds;Parent=MsG0880047728.01.T01
Gene Sequence

>MsG0880047728.01.T01

ATGCCAAAAGTGAGGGAAAGGGAAATGGTGAGGTGTGAAAAGCAATATGGAGAGGAGCCTCCAGTTGTCCTCTCAGACCCTATGGTGTTGGAGATTAATCGTTTGCATAATCTCTTTCATTCTTCAATATTTTTTTTTCTTATTGAATGGATATTTACCCAAAAAAAAAGTATTGACCAGATCATTGTCTCAGTCATGATGAGAACGAAAAAAGTTAAGGATGTAGCAACTTGTCAGAGTGAAATCAAAGCACTAAGGTCGACTGAAGCTCTGAAGGATAAGGCCATAGAAGAGCTGAGAAATGAAGTCGGTAAACTGGACGAGAGACTTAGAGTTACAGAGGATCGTCTTAAGCAAAAGAATATAGAAATCAAGAAACTGACAGACGAAAAGAAAAGTGCAATGGCTGCACAATATGCCGCAGAAGCAACACTTAGAAGGGTGCATGCAAACCAAAAGGATGACGATTCTGTTCCAATTGATAACGCCATTGCCCCTCTTCAAGCTGAGATTACAATGTATAGGAATGAGGAGGAGACCAAAATTTTAGAGAAATCTCGCCGCCAAAAGATTCTTGAAGTTGAAAAGCTTAGCCAAACTATTCAGGAACTTGAGGAAGTCATCTTAGCTAACGGCGCTACAGCTAACGTTGTTCGCGATTACCAAAGACAAGTTTCTGAATTGCAAGATGAGAAAAGGACACTAGAGAGGGAGCTAGCAAGGGTAAAAGTTTCGGCCAATAGAATTGCAAATGGTGAAGCTAATGAGTGGAAGGATGAAAATGACAAGGTCATGCCTGTCAAGCAATGGCTAGAAGAGAGAAGGATTATGCAGACAGAGATGCAAAGGTTGAAAGACAAGCTTGCTATATCAGAGAGAACAGCCAAGGCAGAATCACAGCTGAAGGAAAAACTAAAAGTGAGACTAAAAACAATGGAGGAAGGTTTAAAACATTCTTCAAGTTCAAAAGCAGAAAAGCCTAACATCATAAGTTCCCGAACAGCAAGTGGTGGTCCAAAAAAGAGATCTACATCACAACCAAGAGCATCTGCTATTGGAAGCCCTTTATTTCAACAGACAAATATAAAAAACAACACAAACACTGTTGGGGGAAATTTAAAACTGGAAATTCCTATTAAAATGAAGTATACTTCTGCGGAAAACATTTCAATGCTGAAAGTGAAGCCGAAGAAATCAAAAACAATTGTTGATGTGAATGAAGACTTCCAAAGCAAGAAACCAAATGACTCGTGCAGTGACGATTTGGTGTCAGGTTTTTTGTATGATAGGCTGCAAAGAGAGGTCATTAAATTGAGGAAGTCTTGTGAAACCAAAGACAGTAGTTTGCAAGCTAAAGATGAAGAAATTATGATGTTCATGAAGAAAGTTGATGCACTGACCAAGTCCATGGAAGTAGTAGAGTGGAAGAAAATGAAAAGAGATGCAGCTGCTAGAGAAAAGGAGCTTTCAGCAGCAATAAATTCAGATGATAACAAGAAATACAGAAACACAGACTCCTCCAAAAGGTTAGTGGGCTGA
Protein sequence

>MsG0880047728.01.T01

MPKVREREMVRCEKQYGEEPPVVLSDPMVLEINRLHNLFHSSIFFFLIEWIFTQKKSIDQIIVSVMMRTKKVKDVATCQSEIKALRSTEALKDKAIEELRNEVGKLDERLRVTEDRLKQKNIEIKKLTDEKKSAMAAQYAAEATLRRVHANQKDDDSVPIDNAIAPLQAEITMYRNEEETKILEKSRRQKILEVEKLSQTIQELEEVILANGATANVVRDYQRQVSELQDEKRTLERELARVKVSANRIANGEANEWKDENDKVMPVKQWLEERRIMQTEMQRLKDKLAISERTAKAESQLKEKLKVRLKTMEEGLKHSSSSKAEKPNIISSRTASGGPKKRSTSQPRASAIGSPLFQQTNIKNNTNTVGGNLKLEIPIKMKYTSAENISMLKVKPKKSKTIVDVNEDFQSKKPNDSCSDDLVSGFLYDRLQREVIKLRKSCETKDSSLQAKDEEIMMFMKKVDALTKSMEVVEWKKMKRDAAAREKELSAAINSDDNKKYRNTDSSKRLVG*