AlfalfaGEDB | MsG0880047746.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047746.01.T01	AES92747.1	94.286	105	6	0	1	105	221	325	8.09E-63	203

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047746.01.T01	Q9FWW5	47.674	86	45	0	4	89	460	545	3.88E-11	61.2

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0880043509.01	MsG0880047746.01	0.800451	1.432125e-48	6.670553e-46
MsG0580026340.01	MsG0880047746.01	0.808384	3.180427e-50	1.816012e-47
MsG0280008113.01	MsG0880047746.01	0.800821	1.204016e-48	5.660899e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047746.01.T01	MTR_4g133450	94.286	105	6	1	105	221	325	9.79e-67	203
MsG0880047746.01.T01	MTR_4g134110	95.238	105	5	1	105	457	561	1.79e-65	206
MsG0880047746.01.T01	MTR_2g027130	49.485	97	49	5	101	481	577	2.93e-22	90.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047746.01.T01	AT1G12150	47.674	86	45	0	4	89	460	545	3.96e-12	61.2
MsG0880047746.01.T01	AT1G12150	47.674	86	45	0	4	89	460	545	3.96e-12	61.2

Find 19 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGTTTCCTCAACAGCTTTC+AGG	0.144522	8:-90344147	None:intergenic
CGGCAATTGAACGTGAATTA+AGG	0.320877	8:+90344224	MsG0880047746.01.T01:CDS
ATCGGTGAAGATGTACTATC+AGG	0.427441	8:-90344259	None:intergenic
AGTGAAGGAATACCAAGAAT+TGG	0.430131	8:+90344039	MsG0880047746.01.T01:CDS
TTATCTGAACAATCTGATAT+CGG	0.442943	8:-90344277	None:intergenic
AAAGAAAGAGGCAGCTATCA+TGG	0.460393	8:+90344066	MsG0880047746.01.T01:CDS
CTGCAATGGAGACGGCTTTG+TGG	0.473961	8:+90344173	MsG0880047746.01.T01:CDS
GGCTTTGTGGTATGCAGAGA+AGG	0.504032	8:+90344186	MsG0880047746.01.T01:CDS
GAAGAGTTTGAGTCACTGAA+TGG	0.508477	8:+90344013	MsG0880047746.01.T01:CDS
TGAGGAAACAAAGGCTGCAA+TGG	0.535827	8:+90344159	MsG0880047746.01.T01:CDS
AGCTAACCTGAAAGCTGTTG+AGG	0.545144	8:+90344141	MsG0880047746.01.T01:CDS
AACAAAGGCTGCAATGGAGA+CGG	0.559947	8:+90344165	MsG0880047746.01.T01:CDS
GTCACTGAATGGAAAAGTGA+AGG	0.582811	8:+90344024	MsG0880047746.01.T01:CDS
AGAATTGGCAGAAAAGAAAG+AGG	0.594912	8:+90344054	MsG0880047746.01.T01:CDS
GAAAGCTGTTGAGGAAACAA+AGG	0.603568	8:+90344150	MsG0880047746.01.T01:CDS
GCTATCATGGCTGAGTTGCA+AGG	0.607203	8:+90344079	MsG0880047746.01.T01:CDS
GAAGGCAAATTCTGCAAAAG+CGG	0.610126	8:+90344204	MsG0880047746.01.T01:CDS
AAATGAATTAGACATAAAGG+TGG	0.613977	8:+90344117	MsG0880047746.01.T01:CDS
AGTTGCAAGGAATGTACACA+AGG	0.668978	8:+90344092	MsG0880047746.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GAAAAATGAATTAGACATAA+AGG	+	Chr8:90344114-90344133	MsG0880047746.01.T01:CDS	20.0%
!	AAATGAATTAGACATAAAGG+TGG	+	Chr8:90344117-90344136	MsG0880047746.01.T01:CDS	25.0%
!	TTATCTGAACAATCTGATAT+CGG	-	Chr8:90344280-90344299	None:intergenic	25.0%
	AGAATTGGCAGAAAAGAAAG+AGG	+	Chr8:90344054-90344073	MsG0880047746.01.T01:CDS	35.0%
	AGTGAAGGAATACCAAGAAT+TGG	+	Chr8:90344039-90344058	MsG0880047746.01.T01:CDS	35.0%
!	CTTTCTTTTCTGCCAATTCT+TGG	-	Chr8:90344054-90344073	None:intergenic	35.0%
	AAAGAAAGAGGCAGCTATCA+TGG	+	Chr8:90344066-90344085	MsG0880047746.01.T01:CDS	40.0%
	AGTTGCAAGGAATGTACACA+AGG	+	Chr8:90344092-90344111	MsG0880047746.01.T01:CDS	40.0%
	ATCGGTGAAGATGTACTATC+AGG	-	Chr8:90344262-90344281	None:intergenic	40.0%
	CGGCAATTGAACGTGAATTA+AGG	+	Chr8:90344224-90344243	MsG0880047746.01.T01:CDS	40.0%
	GAAAGCTGTTGAGGAAACAA+AGG	+	Chr8:90344150-90344169	MsG0880047746.01.T01:CDS	40.0%
	GAAGAGTTTGAGTCACTGAA+TGG	+	Chr8:90344013-90344032	MsG0880047746.01.T01:CDS	40.0%
	GAAGGCAAATTCTGCAAAAG+CGG	+	Chr8:90344204-90344223	MsG0880047746.01.T01:CDS	40.0%
	GTCACTGAATGGAAAAGTGA+AGG	+	Chr8:90344024-90344043	MsG0880047746.01.T01:CDS	40.0%
	TTGTTTCCTCAACAGCTTTC+AGG	-	Chr8:90344150-90344169	None:intergenic	40.0%
	AACAAAGGCTGCAATGGAGA+CGG	+	Chr8:90344165-90344184	MsG0880047746.01.T01:CDS	45.0%
	AGCTAACCTGAAAGCTGTTG+AGG	+	Chr8:90344141-90344160	MsG0880047746.01.T01:CDS	45.0%
	TGAGGAAACAAAGGCTGCAA+TGG	+	Chr8:90344159-90344178	MsG0880047746.01.T01:CDS	45.0%
	GCTATCATGGCTGAGTTGCA+AGG	+	Chr8:90344079-90344098	MsG0880047746.01.T01:CDS	50.0%
!	GGCTTTGTGGTATGCAGAGA+AGG	+	Chr8:90344186-90344205	MsG0880047746.01.T01:CDS	50.0%
	CTGCAATGGAGACGGCTTTG+TGG	+	Chr8:90344173-90344192	MsG0880047746.01.T01:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	90344004	90344321	90344004	ID=MsG0880047746.01;Name=MsG0880047746.01
Chr8	mRNA	90344004	90344321	90344004	ID=MsG0880047746.01.T01;Parent=MsG0880047746.01;Name=MsG0880047746.01.T01;_AED=0.49;_eAED=0.49;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|105
Chr8	exon	90344004	90344321	90344004	ID=MsG0880047746.01.T01:exon:2658;Parent=MsG0880047746.01.T01
Chr8	CDS	90344004	90344321	90344004	ID=MsG0880047746.01.T01:cds;Parent=MsG0880047746.01.T01

Gene Sequence	>MsG0880047746.01.T01 ATGTCAAAAGAAGAGTTTGAGTCACTGAATGGAAAAGTGAAGGAATACCAAGAATTGGCAGAAAAGAAAGAGGCAGCTATCATGGCTGAGTTGCAAGGAATGTACACAAGGAAAAATGAATTAGACATAAAGGTGGAAGCTAACCTGAAAGCTGTTGAGGAAACAAAGGCTGCAATGGAGACGGCTTTGTGGTATGCAGAGAAGGCAAATTCTGCAAAAGCGGCAATTGAACGTGAATTAAGGAGATGTCGTCAACCTGATAGTACATCTTCACCGATATCAGATTGTTCAGATAATTCTTCGATGTTGAGCATCTAG
Protein sequence	>MsG0880047746.01.T01 MSKEEFESLNGKVKEYQELAEKKEAAIMAELQGMYTRKNELDIKVEANLKAVEETKAAMETALWYAEKANSAKAAIERELRRCRQPDSTSSPISDCSDNSSMLSI*

M. sativa cultivar ZhongmuNo.1 / MsG0880047746.01