AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880047771.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047771.01.T01 MTR_4g134460 84.091 88 13 1 7 93 50 137 1.83e-47 156
MsG0880047771.01.T01 MTR_6g011860 62.025 79 29 1 16 93 20 98 2.60e-31 113
MsG0880047771.01.T01 MTR_5g040420 56.977 86 36 1 9 93 12 97 1.63e-30 111
MsG0880047771.01.T01 MTR_8g467490 55.814 86 37 1 9 93 12 97 3.76e-30 110
MsG0880047771.01.T01 MTR_5g090970 55.682 88 38 1 7 93 14 101 1.15e-26 99.8
MsG0880047771.01.T01 MTR_4g075980 47.312 93 48 1 5 96 9 101 2.08e-26 99.0
MsG0880047771.01.T01 MTR_4g089135 55.696 79 34 1 16 93 20 98 9.92e-26 97.8
MsG0880047771.01.T01 MTR_2g080010 55.696 79 34 1 16 93 20 98 2.57e-25 97.1
MsG0880047771.01.T01 MTR_5g090970 55.682 88 37 2 7 93 14 100 2.80e-25 96.3
MsG0880047771.01.T01 MTR_7g085220 54.430 79 35 1 16 93 13 91 2.84e-25 96.7
MsG0880047771.01.T01 MTR_2g079990 55.696 79 34 1 16 93 21 99 6.80e-25 95.9
MsG0880047771.01.T01 MTR_2g079990 55.696 79 34 1 16 93 21 99 1.32e-24 95.5
MsG0880047771.01.T01 MTR_3g088110 54.430 79 35 1 16 93 12 90 3.89e-24 92.8
MsG0880047771.01.T01 MTR_8g059170 55.696 79 34 1 16 93 19 97 5.96e-24 92.8
MsG0880047771.01.T01 MTR_3g064580 51.852 81 36 2 16 93 11 91 2.84e-23 92.8
MsG0880047771.01.T01 MTR_3g096920 53.165 79 36 1 16 93 12 90 3.52e-23 90.9
MsG0880047771.01.T01 MTR_3g096920 53.165 79 36 1 16 93 12 90 4.78e-23 90.1
MsG0880047771.01.T01 MTR_5g014300 49.383 81 38 2 16 93 11 91 1.15e-22 89.7
MsG0880047771.01.T01 MTR_2157s0010 51.899 79 37 1 16 93 13 91 4.46e-22 87.0
MsG0880047771.01.T01 MTR_2g068880 51.685 89 41 2 7 93 18 106 4.78e-22 87.8
MsG0880047771.01.T01 MTR_8g094580 51.899 79 37 1 16 93 12 90 5.02e-22 87.0
MsG0880047771.01.T01 MTR_5g076850 50.617 81 37 2 16 93 11 91 7.86e-22 88.6
MsG0880047771.01.T01 MTR_2g068920 51.685 89 41 2 7 93 17 105 8.47e-22 86.7
MsG0880047771.01.T01 MTR_2g064090 49.383 81 38 2 16 93 11 91 9.13e-22 87.4
MsG0880047771.01.T01 MTR_2g093810 48.101 79 40 1 16 93 19 97 1.09e-21 87.0
MsG0880047771.01.T01 MTR_5g041940 49.367 79 39 1 16 93 8 86 2.56e-21 84.7
MsG0880047771.01.T01 MTR_7g100990 49.367 79 39 1 16 93 16 94 6.29e-21 84.7
MsG0880047771.01.T01 MTR_3g116070 50.000 80 38 1 16 93 28 107 1.16e-20 84.3
MsG0880047771.01.T01 MTR_6g084430 48.718 78 39 1 16 92 10 87 2.50e-20 83.2
MsG0880047771.01.T01 MTR_4g108760 49.367 79 39 1 16 93 22 100 4.83e-20 82.8
MsG0880047771.01.T01 MTR_7g011120 47.561 82 42 1 13 93 10 91 7.38e-20 81.6
MsG0880047771.01.T01 MTR_2g078700 51.899 79 37 1 16 93 28 106 8.41e-20 82.4
MsG0880047771.01.T01 MTR_4g098630 48.148 81 39 2 16 93 11 91 8.79e-20 81.6
MsG0880047771.01.T01 MTR_5g069030 49.383 81 38 2 16 93 30 110 1.31e-19 82.4
MsG0880047771.01.T01 MTR_4g081870 49.367 79 39 1 16 93 14 92 2.27e-19 80.1
MsG0880047771.01.T01 MTR_6g032770 46.341 82 43 1 13 93 20 101 2.95e-19 80.9
MsG0880047771.01.T01 MTR_7g011130 46.341 82 43 1 13 93 21 102 3.35e-19 80.5
MsG0880047771.01.T01 MTR_6g477900 45.679 81 41 2 16 93 11 91 3.74e-19 79.0
MsG0880047771.01.T01 MTR_3g435150 48.101 79 40 1 16 93 21 99 5.02e-19 79.3
MsG0880047771.01.T01 MTR_4g101680 43.902 82 42 2 16 93 12 93 6.99e-19 79.7
MsG0880047771.01.T01 MTR_5g012080 42.683 82 43 2 16 93 12 93 7.03e-19 79.7
MsG0880047771.01.T01 MTR_6g012670 49.367 79 39 1 16 93 25 103 7.49e-19 79.3
MsG0880047771.01.T01 MTR_7g085260 46.835 79 41 1 16 93 22 100 7.67e-19 79.7
MsG0880047771.01.T01 MTR_1g097300 44.444 81 42 2 16 93 24 104 9.33e-19 79.7
MsG0880047771.01.T01 MTR_3g109340 48.718 78 39 1 16 92 27 104 1.35e-18 79.0
MsG0880047771.01.T01 MTR_3g109340 48.718 78 39 1 16 92 27 104 1.37e-18 79.0
MsG0880047771.01.T01 MTR_4g036030 41.463 82 44 2 16 93 12 93 1.47e-18 78.6
MsG0880047771.01.T01 MTR_3g070030 44.304 79 43 1 16 93 24 102 1.75e-18 78.6
MsG0880047771.01.T01 MTR_8g063550 45.679 81 41 2 16 93 11 91 2.81e-18 77.8
MsG0880047771.01.T01 MTR_3g093050 44.444 81 42 2 16 93 27 107 3.40e-18 78.2
MsG0880047771.01.T01 MTR_3g093050 44.444 81 42 2 16 93 27 107 3.96e-18 78.2
MsG0880047771.01.T01 MTR_8g063550 45.679 81 41 2 16 93 11 91 4.18e-18 77.8
MsG0880047771.01.T01 MTR_8g063550 45.679 81 41 2 16 93 11 91 4.27e-18 77.8
MsG0880047771.01.T01 MTR_2g064470 48.750 80 36 2 16 93 16 92 4.92e-18 76.6
MsG0880047771.01.T01 MTR_3g093040 43.210 81 43 2 16 93 113 193 5.94e-18 76.6
MsG0880047771.01.T01 MTR_8g093580 43.210 81 43 2 16 93 11 91 7.41e-18 77.0
MsG0880047771.01.T01 MTR_2g086880 40.217 92 53 2 4 93 11 102 1.90e-17 75.9
MsG0880047771.01.T01 MTR_1g008740 46.835 79 40 2 16 92 18 96 2.04e-17 75.5
MsG0880047771.01.T01 MTR_0036s0150 46.341 82 38 3 16 92 10 90 2.37e-17 75.9
MsG0880047771.01.T01 MTR_2g086880 40.217 92 53 2 4 93 11 102 2.44e-17 75.9
MsG0880047771.01.T01 MTR_1g008740 46.835 79 40 2 16 92 32 110 2.74e-17 75.1
MsG0880047771.01.T01 MTR_1g090720 46.914 81 40 2 16 93 47 127 3.42e-17 75.5
MsG0880047771.01.T01 MTR_7g097090 45.570 79 42 1 16 93 15 93 3.80e-17 74.3
MsG0880047771.01.T01 MTR_3g096140 39.560 91 52 2 5 92 1 91 9.36e-17 73.6
MsG0880047771.01.T01 MTR_4g035590 41.463 82 44 2 16 93 13 94 1.05e-16 73.6
MsG0880047771.01.T01 MTR_5g021710 40.244 82 45 2 16 93 13 94 1.32e-16 73.2
MsG0880047771.01.T01 MTR_1g069805 45.570 79 42 1 16 93 15 93 1.56e-16 72.4
MsG0880047771.01.T01 MTR_1g090723 42.683 82 41 3 16 92 13 93 2.05e-16 73.2
MsG0880047771.01.T01 MTR_1g090723 42.683 82 41 3 16 92 13 93 2.05e-16 73.2
MsG0880047771.01.T01 MTR_8g024480 39.024 82 46 2 16 93 20 101 2.12e-16 73.2
MsG0880047771.01.T01 MTR_1g096430 39.024 82 46 2 16 93 12 93 2.80e-16 72.4
MsG0880047771.01.T01 MTR_1g090723 41.975 81 43 2 16 92 13 93 2.92e-16 72.8
MsG0880047771.01.T01 MTR_3g096140 39.130 92 53 2 5 93 1 92 7.40e-16 70.5
MsG0880047771.01.T01 MTR_2g086690 44.318 88 42 3 16 96 9 96 7.45e-16 70.1
MsG0880047771.01.T01 MTR_2g062730 41.463 82 44 2 16 93 15 96 1.19e-15 70.5
MsG0880047771.01.T01 MTR_8g023840 43.902 82 42 2 16 93 16 97 1.63e-15 70.5
MsG0880047771.01.T01 MTR_8g076110 40.244 82 45 2 16 93 11 92 4.65e-15 68.9
MsG0880047771.01.T01 MTR_7g105170 37.500 88 48 3 16 96 9 96 5.66e-15 68.2
MsG0880047771.01.T01 MTR_4g052620 39.362 94 51 2 9 96 3 96 1.07e-14 67.4
MsG0880047771.01.T01 MTR_8g023900 38.889 90 51 2 8 93 9 98 1.07e-14 68.2
MsG0880047771.01.T01 MTR_8g023900 40.698 86 47 2 12 93 194 279 8.26e-12 60.1
MsG0880047771.01.T01 MTR_7g005280 44.304 79 40 2 16 93 20 95 5.71e-13 62.8
MsG0880047771.01.T01 MTR_8g023860 37.079 89 52 2 9 93 10 98 1.25e-12 62.4
MsG0880047771.01.T01 MTR_8g094580 69.444 36 11 0 58 93 1 36 1.68e-12 61.2
MsG0880047771.01.T01 MTR_3g098810 39.744 78 44 2 17 93 20 95 1.98e-12 60.1
MsG0880047771.01.T01 MTR_3g098810 39.744 78 44 2 17 93 20 95 3.33e-12 60.5
MsG0880047771.01.T01 MTR_8g023930 39.024 82 46 2 16 93 29 110 1.08e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047771.01.T01 AT2G02450 84.810 79 11 1 16 93 56 134 8.52e-43 143
MsG0880047771.01.T01 AT2G02450 84.810 79 11 1 16 93 56 134 1.09e-42 144
MsG0880047771.01.T01 AT5G39820 61.628 86 32 1 9 93 18 103 1.22e-30 110
MsG0880047771.01.T01 AT1G26870 58.140 86 35 1 9 93 14 99 1.47e-30 112
MsG0880047771.01.T01 AT2G17040 55.056 89 39 1 9 96 4 92 1.72e-28 103
MsG0880047771.01.T01 AT2G43000 53.409 88 40 1 7 93 14 101 2.48e-26 98.2
MsG0880047771.01.T01 AT3G15510 56.962 79 33 1 16 93 22 100 6.95e-26 98.6
MsG0880047771.01.T01 AT1G52880 56.962 79 33 1 16 93 22 100 1.03e-25 97.4
MsG0880047771.01.T01 AT3G04070 55.696 79 34 1 16 93 15 93 3.46e-25 96.7
MsG0880047771.01.T01 AT5G08790 51.807 83 39 1 13 94 9 91 4.61e-25 95.1
MsG0880047771.01.T01 AT3G04070 55.696 79 34 1 16 93 15 93 5.88e-25 96.3
MsG0880047771.01.T01 AT4G27410 55.696 79 34 1 16 93 19 97 7.75e-25 94.7
MsG0880047771.01.T01 AT1G01720 56.250 80 34 1 16 94 12 91 1.52e-24 94.0
MsG0880047771.01.T01 AT3G15500 55.696 79 34 1 16 93 19 97 2.11e-24 94.0
MsG0880047771.01.T01 AT5G63790 53.659 82 37 1 13 93 52 133 4.27e-24 93.2
MsG0880047771.01.T01 AT5G63790 53.659 82 37 1 13 93 62 143 5.15e-24 93.2
MsG0880047771.01.T01 AT1G61110 54.430 79 35 1 16 93 21 99 7.20e-24 92.8
MsG0880047771.01.T01 AT1G52890 55.696 79 34 1 16 93 19 97 1.17e-23 92.0
MsG0880047771.01.T01 AT1G77450 51.250 80 38 1 16 94 15 94 1.19e-22 88.2
MsG0880047771.01.T01 AT1G65910 48.148 81 39 2 16 93 11 91 7.82e-22 88.6
MsG0880047771.01.T01 AT1G76420 51.899 79 37 1 16 93 27 105 7.90e-22 87.4
MsG0880047771.01.T01 AT5G17260 48.148 81 39 2 16 93 11 91 8.26e-22 88.6
MsG0880047771.01.T01 AT3G03200 48.148 81 39 2 16 93 11 91 1.64e-21 87.4
MsG0880047771.01.T01 AT3G15170 50.633 79 38 1 16 93 25 103 3.47e-21 85.5
MsG0880047771.01.T01 AT1G69490 50.000 80 39 1 16 94 14 93 3.79e-21 84.7
MsG0880047771.01.T01 AT5G61430 50.633 79 38 1 16 93 21 99 6.64e-21 85.1
MsG0880047771.01.T01 AT4G27410 45.833 96 34 2 16 93 19 114 6.84e-21 84.7
MsG0880047771.01.T01 AT4G17980 46.341 82 41 2 16 94 11 92 7.77e-21 83.6
MsG0880047771.01.T01 AT4G17980 46.914 81 40 2 16 93 11 91 9.90e-21 84.0
MsG0880047771.01.T01 AT5G07680 53.165 79 36 1 16 93 8 86 1.20e-20 84.0
MsG0880047771.01.T01 AT5G07680 53.165 79 36 1 16 93 22 100 1.49e-20 84.0
MsG0880047771.01.T01 AT3G04060 49.398 83 33 2 16 93 25 103 1.74e-20 84.0
MsG0880047771.01.T01 AT1G34180 45.679 81 41 2 16 93 21 101 2.30e-20 84.3
MsG0880047771.01.T01 AT4G35580 50.617 81 37 2 16 93 14 94 2.40e-20 84.3
MsG0880047771.01.T01 AT1G34180 45.679 81 41 2 16 93 21 101 2.48e-20 84.3
MsG0880047771.01.T01 AT5G46590 49.383 81 38 2 16 93 11 91 2.91e-20 82.4
MsG0880047771.01.T01 AT4G35580 50.617 81 37 2 16 93 14 94 2.94e-20 84.0
MsG0880047771.01.T01 AT5G46590 49.383 81 38 2 16 93 11 91 2.98e-20 82.8
MsG0880047771.01.T01 AT4G35580 50.617 81 37 2 16 93 14 94 3.09e-20 84.0
MsG0880047771.01.T01 AT3G29035 50.633 79 38 1 16 93 29 107 3.36e-20 82.8
MsG0880047771.01.T01 AT3G18400 51.282 78 37 1 16 92 10 87 4.13e-20 82.4
MsG0880047771.01.T01 AT1G34190 45.679 81 41 2 16 93 21 101 5.35e-20 83.6
MsG0880047771.01.T01 AT1G54330 48.148 81 39 2 16 93 11 91 7.15e-20 81.6
MsG0880047771.01.T01 AT1G62700 45.122 82 41 2 16 93 12 93 9.53e-20 82.4
MsG0880047771.01.T01 AT1G62700 45.122 82 41 2 16 93 12 93 9.53e-20 82.4
MsG0880047771.01.T01 AT5G18270 50.633 79 38 1 16 93 26 104 1.01e-19 81.6
MsG0880047771.01.T01 AT5G64060 46.250 80 40 2 16 92 11 90 1.38e-19 81.6
MsG0880047771.01.T01 AT5G18270 50.633 79 38 1 16 93 26 104 2.23e-19 80.9
MsG0880047771.01.T01 AT5G53950 50.633 79 38 1 16 93 22 100 2.26e-19 81.3
MsG0880047771.01.T01 AT1G12260 45.122 82 41 2 16 93 12 93 2.45e-19 81.3
MsG0880047771.01.T01 AT4G28530 42.105 95 38 1 16 93 15 109 4.68e-19 79.7
MsG0880047771.01.T01 AT1G12260 45.122 82 41 2 16 93 12 93 5.48e-19 79.7
MsG0880047771.01.T01 AT2G24430 43.678 87 48 1 7 92 12 98 6.23e-19 79.3
MsG0880047771.01.T01 AT2G24430 43.678 87 48 1 7 92 12 98 6.23e-19 79.3
MsG0880047771.01.T01 AT5G09330 45.000 80 41 2 16 92 11 90 9.68e-19 79.7
MsG0880047771.01.T01 AT5G09330 45.000 80 41 2 16 92 11 90 9.68e-19 79.7
MsG0880047771.01.T01 AT5G09330 45.000 80 41 2 16 92 11 90 9.68e-19 79.7
MsG0880047771.01.T01 AT5G09330 45.000 80 41 2 16 92 11 90 9.68e-19 79.7
MsG0880047771.01.T01 AT5G09330 45.000 80 41 2 16 92 11 90 9.68e-19 79.7
MsG0880047771.01.T01 AT4G28530 42.105 95 38 1 16 93 15 109 1.13e-18 79.0
MsG0880047771.01.T01 AT3G17730 43.902 82 43 2 16 94 11 92 1.43e-18 77.4
MsG0880047771.01.T01 AT3G10500 43.750 80 42 2 16 92 14 93 1.55e-18 79.3
MsG0880047771.01.T01 AT1G56010 45.000 80 42 1 16 93 24 103 1.84e-18 78.2
MsG0880047771.01.T01 AT1G32510 48.148 81 39 2 16 93 11 91 2.30e-18 77.4
MsG0880047771.01.T01 AT5G39610 46.067 89 47 1 7 94 16 104 2.48e-18 77.4
MsG0880047771.01.T01 AT1G33060 45.679 81 41 2 16 93 29 109 2.97e-18 78.6
MsG0880047771.01.T01 AT1G33060 45.679 81 41 2 16 93 29 109 3.00e-18 78.6
MsG0880047771.01.T01 AT5G04410 45.000 80 41 2 16 92 14 93 3.53e-18 78.2
MsG0880047771.01.T01 AT3G49530 40.426 94 53 2 3 93 5 98 3.96e-18 78.2
MsG0880047771.01.T01 AT5G22380 43.158 95 47 3 9 96 3 97 4.06e-18 76.3
MsG0880047771.01.T01 AT1G34180 38.710 93 42 2 16 93 21 113 4.83e-18 77.8
MsG0880047771.01.T01 AT1G34180 38.710 93 42 2 16 93 21 113 4.99e-18 77.8
MsG0880047771.01.T01 AT3G49530 40.426 94 53 2 3 93 5 98 6.44e-18 77.4
MsG0880047771.01.T01 AT3G61910 40.244 82 45 2 16 93 16 97 9.83e-18 76.3
MsG0880047771.01.T01 AT5G66300 37.374 99 55 3 3 94 1 99 1.37e-17 75.5
MsG0880047771.01.T01 AT3G12977 45.570 79 42 1 16 93 23 101 4.38e-17 73.9
MsG0880047771.01.T01 AT4G10350 41.463 82 44 2 16 93 14 95 6.28e-17 74.3
MsG0880047771.01.T01 AT1G71930 41.463 82 44 2 16 93 14 95 8.89e-17 73.6
MsG0880047771.01.T01 AT1G71930 41.463 82 44 2 16 93 14 95 8.89e-17 73.6
MsG0880047771.01.T01 AT3G10480 47.561 82 38 3 16 93 32 112 1.05e-16 73.9
MsG0880047771.01.T01 AT3G10480 47.561 82 38 3 16 93 32 112 1.11e-16 73.9
MsG0880047771.01.T01 AT2G46770 39.024 82 46 2 16 93 21 102 1.13e-16 73.6
MsG0880047771.01.T01 AT3G10490 46.988 83 39 3 16 94 32 113 1.26e-16 72.0
MsG0880047771.01.T01 AT1G33280 42.683 82 43 2 16 93 13 94 1.28e-16 73.2
MsG0880047771.01.T01 AT5G62380 42.683 82 43 2 16 93 12 93 1.36e-16 73.2
MsG0880047771.01.T01 AT5G62380 42.683 82 43 2 16 93 12 93 1.36e-16 73.2
MsG0880047771.01.T01 AT4G36160 41.463 82 44 2 16 93 15 96 1.57e-16 73.2
MsG0880047771.01.T01 AT4G36160 41.463 82 44 2 16 93 15 96 1.57e-16 73.2
MsG0880047771.01.T01 AT5G24590 44.444 81 42 2 16 93 18 98 1.70e-16 73.2
MsG0880047771.01.T01 AT4G36160 41.463 82 44 2 16 93 19 100 1.81e-16 73.2
MsG0880047771.01.T01 AT2G18060 40.244 82 45 2 16 93 14 95 2.90e-16 72.4
MsG0880047771.01.T01 AT2G18060 40.244 82 45 2 16 93 14 95 2.90e-16 72.4
MsG0880047771.01.T01 AT3G10490 47.561 82 38 3 16 93 32 112 4.75e-16 72.0
MsG0880047771.01.T01 AT1G32770 39.024 82 46 2 16 93 21 102 7.20e-16 71.2
MsG0880047771.01.T01 AT3G44350 41.573 89 42 3 16 96 10 96 8.32e-16 68.6
MsG0880047771.01.T01 AT3G12910 50.617 81 36 3 16 93 26 105 9.58e-16 70.5
MsG0880047771.01.T01 AT5G13180 49.367 79 36 2 16 93 19 94 1.03e-15 70.1
MsG0880047771.01.T01 AT1G79580 41.463 82 44 2 16 93 22 103 1.48e-15 70.5
MsG0880047771.01.T01 AT1G79580 41.463 82 44 2 16 93 22 103 1.48e-15 70.5
MsG0880047771.01.T01 AT1G79580 41.463 82 44 2 16 93 22 103 1.48e-15 70.5
MsG0880047771.01.T01 AT1G79580 41.463 82 44 2 16 93 22 103 1.48e-15 70.5
MsG0880047771.01.T01 AT1G79580 41.463 82 44 2 16 93 22 103 1.48e-15 70.5
MsG0880047771.01.T01 AT3G44350 41.573 89 42 3 16 96 10 96 2.82e-15 68.6
MsG0880047771.01.T01 AT2G33480 48.101 79 37 2 16 93 20 95 2.97e-15 68.9
MsG0880047771.01.T01 AT2G33480 48.101 79 37 2 16 93 20 95 2.97e-15 68.9
MsG0880047771.01.T01 AT2G27300 36.957 92 56 2 4 93 7 98 6.63e-15 68.6
MsG0880047771.01.T01 AT5G22290 35.165 91 57 2 6 94 16 106 5.39e-14 66.2
MsG0880047771.01.T01 AT5G04395 31.959 97 48 2 16 96 33 127 6.13e-13 61.6
MsG0880047771.01.T01 AT3G44290 37.500 80 48 2 16 93 19 98 6.94e-13 62.8
MsG0880047771.01.T01 AT3G44290 34.483 87 55 2 10 94 13 99 7.08e-13 62.0
MsG0880047771.01.T01 AT4G01550 39.024 82 46 2 16 93 9 90 3.52e-12 61.2
MsG0880047771.01.T01 AT1G32870 41.053 95 51 3 2 93 1 93 1.00e-11 59.7
MsG0880047771.01.T01 AT1G32870 41.053 95 51 3 2 93 1 93 1.12e-11 59.7
MsG0880047771.01.T01 AT1G32870 40.625 96 52 3 1 93 36 129 1.17e-11 59.7

Find 26 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGTAACAACTTCTGGTTAT+TGG 0.211019 8:+90631868 MsG0880047771.01.T01:CDS
CCTAATCGTGTAACAACTTC+TGG 0.308486 8:+90631861 MsG0880047771.01.T01:CDS
CCAGAAGTTGTTACACGATT+AGG 0.317297 8:-90631861 None:intergenic
CTGGTTATTGGAAAGCAAAC+AGG 0.400340 8:+90631880 MsG0880047771.01.T01:CDS
GCTAGACCCTTGGAACTTCC+TGG 0.406610 8:+90631437 MsG0880047771.01.T01:CDS
AGCATGACGTGGTTTATGCC+AGG 0.468075 8:+90631309 MsG0880047771.01.T01:CDS
GATCTTTATCGCTAGACCCT+TGG 0.471921 8:+90631427 MsG0880047771.01.T01:CDS
TCCCCTCGACCTTACGGCGA+AGG 0.480275 8:-90631373 None:intergenic
CAAGCTCTTCTTCAGTTGGA+TGG 0.486659 8:-90631340 None:intergenic
AGAGTTCTACCTTCGCCGTA+AGG 0.493247 8:+90631364 MsG0880047771.01.T01:CDS
CTACCTTCGCCGTAAGGTCG+AGG 0.506493 8:+90631370 MsG0880047771.01.T01:CDS
TCTACAAGCTCTTCTTCAGT+TGG 0.506937 8:-90631344 None:intergenic
AACGTTTCCCCTCGACCTTA+CGG 0.515160 8:-90631379 None:intergenic
TTTGGCAGCAATCGGAGAGA+AGG 0.516877 8:+90631788 MsG0880047771.01.T01:CDS
TGAGCAGCTTTGGCAGCAAT+CGG 0.520334 8:+90631780 MsG0880047771.01.T01:intron
GAAAGCAAACAGGAGCAGAT+AGG 0.530604 8:+90631890 MsG0880047771.01.T01:CDS
AGTTGGATGGAAACGAACCC+TGG 0.547333 8:-90631327 None:intergenic
GCATGACGTGGTTTATGCCA+GGG 0.551597 8:+90631310 MsG0880047771.01.T01:CDS
TACCTTCGCCGTAAGGTCGA+GGG 0.558656 8:+90631371 MsG0880047771.01.T01:CDS
ATATATACCAGGAAGTTCCA+AGG 0.577889 8:-90631444 None:intergenic
TATATACCAGGAAGTTCCAA+GGG 0.592048 8:-90631443 None:intergenic
CGAGATAGAAAGTATCGAAA+CGG 0.593685 8:+90631831 MsG0880047771.01.T01:CDS
ACCTTCGCCGTAAGGTCGAG+GGG 0.599909 8:+90631372 MsG0880047771.01.T01:CDS
TTTCGATACTTTCTATCTCG+AGG 0.612931 8:-90631828 None:intergenic
CAGCAATCGGAGAGAAGGAG+TGG 0.646393 8:+90631793 MsG0880047771.01.T01:CDS
GATGATGATCGAGCATGACG+TGG 0.731733 8:+90631298 MsG0880047771.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATAATAAATATATACC+AGG - Chr8:90631458-90631477 None:intergenic 10.0%
!! TATATTATTAAATTCAAAGA+GGG - Chr8:90631583-90631602 None:intergenic 10.0%
!! TTATATTATTAAATTCAAAG+AGG - Chr8:90631584-90631603 None:intergenic 10.0%
!!! TTTTTGATAAATAACTATCT+TGG + Chr8:90631613-90631632 MsG0880047771.01.T01:intron 15.0%
!! AAATATGAAGCAAATAAGTA+AGG - Chr8:90631642-90631661 None:intergenic 20.0%
!! AATATGAAGCAAATAAGTAA+GGG - Chr8:90631641-90631660 None:intergenic 20.0%
!!! TTAATTATGTGATGATTTTG+AGG + Chr8:90631726-90631745 MsG0880047771.01.T01:intron 20.0%
! TATTAAATTCAAAGAGGGAT+GGG - Chr8:90631578-90631597 None:intergenic 25.0%
! TTATTAAATTCAAAGAGGGA+TGG - Chr8:90631579-90631598 None:intergenic 25.0%
!! ATTATGTGATGATTTTGAGG+TGG + Chr8:90631729-90631748 MsG0880047771.01.T01:intron 30.0%
ATATATACCAGGAAGTTCCA+AGG - Chr8:90631447-90631466 None:intergenic 35.0%
TATATACCAGGAAGTTCCAA+GGG - Chr8:90631446-90631465 None:intergenic 35.0%
TTTCGATACTTTCTATCTCG+AGG - Chr8:90631831-90631850 None:intergenic 35.0%
! GTGTAACAACTTCTGGTTAT+TGG + Chr8:90631868-90631887 MsG0880047771.01.T01:CDS 35.0%
! TATATGATGATGAGCAGCTT+TGG + Chr8:90631770-90631789 MsG0880047771.01.T01:intron 35.0%
!! CGAGATAGAAAGTATCGAAA+CGG + Chr8:90631831-90631850 MsG0880047771.01.T01:CDS 35.0%
!! TCCAGAATTGCTTTAGGAAA+GGG - Chr8:90631541-90631560 None:intergenic 35.0%
!! TTCCAGAATTGCTTTAGGAA+AGG - Chr8:90631542-90631561 None:intergenic 35.0%
AAGGGTTCCAGAATTGCTTT+AGG - Chr8:90631547-90631566 None:intergenic 40.0%
AGAGGGATGGGAACAAAAAA+AGG - Chr8:90631566-90631585 None:intergenic 40.0%
CCAGAAGTTGTTACACGATT+AGG - Chr8:90631864-90631883 None:intergenic 40.0%
CCTAATCGTGTAACAACTTC+TGG + Chr8:90631861-90631880 MsG0880047771.01.T01:CDS 40.0%
CTGGTTATTGGAAAGCAAAC+AGG + Chr8:90631880-90631899 MsG0880047771.01.T01:CDS 40.0%
GAGGGATGGGAACAAAAAAA+GGG - Chr8:90631565-90631584 None:intergenic 40.0%
TCTACAAGCTCTTCTTCAGT+TGG - Chr8:90631347-90631366 None:intergenic 40.0%
!! CCAGAATTGCTTTAGGAAAG+GGG - Chr8:90631540-90631559 None:intergenic 40.0%
CAAGCTCTTCTTCAGTTGGA+TGG - Chr8:90631343-90631362 None:intergenic 45.0%
CCCCTTTCCTAAAGCAATTC+TGG + Chr8:90631537-90631556 MsG0880047771.01.T01:intron 45.0%
GATCTTTATCGCTAGACCCT+TGG + Chr8:90631427-90631446 MsG0880047771.01.T01:CDS 45.0%
AACGTTTCCCCTCGACCTTA+CGG - Chr8:90631382-90631401 None:intergenic 50.0%
AGAGTTCTACCTTCGCCGTA+AGG + Chr8:90631364-90631383 MsG0880047771.01.T01:CDS 50.0%
AGCATGACGTGGTTTATGCC+AGG + Chr8:90631309-90631328 MsG0880047771.01.T01:CDS 50.0%
AGTTGGATGGAAACGAACCC+TGG - Chr8:90631330-90631349 None:intergenic 50.0%
GATGATGATCGAGCATGACG+TGG + Chr8:90631298-90631317 MsG0880047771.01.T01:CDS 50.0%
GCATGACGTGGTTTATGCCA+GGG + Chr8:90631310-90631329 MsG0880047771.01.T01:CDS 50.0%
TTTGGCAGCAATCGGAGAGA+AGG + Chr8:90631788-90631807 MsG0880047771.01.T01:CDS 50.0%
! TGAGCAGCTTTGGCAGCAAT+CGG + Chr8:90631780-90631799 MsG0880047771.01.T01:intron 50.0%
CAGCAATCGGAGAGAAGGAG+TGG + Chr8:90631793-90631812 MsG0880047771.01.T01:CDS 55.0%
GCTAGACCCTTGGAACTTCC+TGG + Chr8:90631437-90631456 MsG0880047771.01.T01:CDS 55.0%
TACCTTCGCCGTAAGGTCGA+GGG + Chr8:90631371-90631390 MsG0880047771.01.T01:CDS 55.0%
ACCTTCGCCGTAAGGTCGAG+GGG + Chr8:90631372-90631391 MsG0880047771.01.T01:CDS 60.0%
CTACCTTCGCCGTAAGGTCG+AGG + Chr8:90631370-90631389 MsG0880047771.01.T01:CDS 60.0%
TCCCCTCGACCTTACGGCGA+AGG - Chr8:90631376-90631395 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr8 gene 90631290 90631911 90631290 ID=MsG0880047771.01;Name=MsG0880047771.01
Chr8 mRNA 90631290 90631911 90631290 ID=MsG0880047771.01.T01;Parent=MsG0880047771.01;Name=MsG0880047771.01.T01;_AED=0.40;_eAED=0.40;_QI=0|0|0|1|1|1|2|0|97
Chr8 exon 90631290 90631458 90631290 ID=MsG0880047771.01.T01:exon:16166;Parent=MsG0880047771.01.T01
Chr8 exon 90631787 90631911 90631787 ID=MsG0880047771.01.T01:exon:16167;Parent=MsG0880047771.01.T01
Chr8 CDS 90631290 90631458 90631290 ID=MsG0880047771.01.T01:cds;Parent=MsG0880047771.01.T01
Chr8 CDS 90631787 90631911 90631787 ID=MsG0880047771.01.T01:cds;Parent=MsG0880047771.01.T01
Gene Sequence

>MsG0880047771.01.T01

ATGATGATGATGATGATCGAGCATGACGTGGTTTATGCCAGGGTTCGTTTCCATCCAACTGAAGAAGAGCTTGTAGAGTTCTACCTTCGCCGTAAGGTCGAGGGGAAACGTTTCAACGTTGAGCTATTACTTTTCTCGATCTTTATCGCTAGACCCTTGGAACTTCCTGCTTTGGCAGCAATCGGAGAGAAGGAGTGGTACTTTTATGTGCCTCGAGATAGAAAGTATCGAAACGGTGATCGTCCTAATCGTGTAACAACTTCTGGTTATTGGAAAGCAAACAGGAGCAGATAG
Protein sequence

>MsG0880047771.01.T01

MMMMMIEHDVVYARVRFHPTEEELVEFYLRRKVEGKRFNVELLLFSIFIARPLELPALAAIGEKEWYFYVPRDRKYRNGDRPNRVTTSGYWKANRSR*