AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880047772.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047772.01.T01 MTR_4g134460 84.043 188 24 3 1 186 245 428 3.54e-102 301
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 52 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGTTGATCATCAAGTCTT+TGG 0.303708 8:-90632905 None:intergenic
AAGGAGGTTGGAAATTGATT+TGG 0.344511 8:-90633357 None:intergenic
TGAAGCTGAGACTGGGAAAA+TGG 0.347823 8:-90633160 None:intergenic
GCTGCCGCTGAAGCTGAGAC+TGG 0.355613 8:-90633168 None:intergenic
GTAGACAAATGATTGGGATT+TGG 0.373594 8:-90633312 None:intergenic
ATTGGGAATGGGATTGTTGT+TGG 0.407420 8:+90632876 None:intergenic
TCTTCTGAACGGTAATTAGT+AGG 0.410368 8:-90633015 None:intergenic
GATCATGTGACAACCACTCT+TGG 0.423069 8:+90632964 MsG0880047772.01.T01:CDS
GATCAACATAGTTAACAAAC+TGG 0.432160 8:+90632918 MsG0880047772.01.T01:CDS
TGCAGCACTGGTAGCAGTAT+TGG 0.434071 8:-90633205 None:intergenic
TTGGGGTGATTATGATCCTC+AGG 0.436862 8:-90633411 None:intergenic
CAGCAATGTAGACAAATGAT+TGG 0.439526 8:-90633319 None:intergenic
GGAGGTGTTGTACTACTGTT+GGG 0.448162 8:-90633429 None:intergenic
TGATGATGGATGATCTCAAT+AGG 0.454191 8:+90633229 MsG0880047772.01.T01:CDS
TCTCTGACCGACTGTGGGAC+TGG 0.472209 8:+90633379 MsG0880047772.01.T01:CDS
TGATTATGATCCTCAGGGAT+TGG 0.472665 8:-90633405 None:intergenic
AGGAGGAGTATTGCTGTACT+TGG 0.484755 8:-90632992 None:intergenic
ATCATGTGACAACCACTCTT+GGG 0.489095 8:+90632965 MsG0880047772.01.T01:CDS
AGTAATTAGTGATTACTTGA+AGG 0.490931 8:-90633450 None:intergenic
GGCGCTGCCGCCACCACAAG+CGG 0.493139 8:-90633139 None:intergenic
GATTTGGATGATTGAGATGT+GGG 0.497103 8:-90633296 None:intergenic
CTGCCGCTGAAGCTGAGACT+GGG 0.499254 8:-90633167 None:intergenic
GGAAATTGATTTGGTAACAT+TGG 0.503521 8:-90633348 None:intergenic
TTAGTCTCCGCTTGTGGTGG+CGG 0.506486 8:+90633132 MsG0880047772.01.T01:CDS
ATCCATCATCATTGCAGCAC+TGG 0.512112 8:-90633217 None:intergenic
AGCAATGTAGACAAATGATT+GGG 0.519527 8:-90633318 None:intergenic
TACCGTTCAGAAGATGAAGA+AGG 0.528026 8:+90633024 MsG0880047772.01.T01:CDS
CCTCCTTCTCTGACCGACTG+TGG 0.530397 8:+90633373 MsG0880047772.01.T01:CDS
AGGAGGTGTTGTACTACTGT+TGG 0.537736 8:-90633430 None:intergenic
CCACAGTCGGTCAGAGAAGG+AGG 0.546229 8:-90633373 None:intergenic
GCATATTGTACTAGTTGCCT+AGG 0.547334 8:-90633090 None:intergenic
GTCCCACAGTCGGTCAGAGA+AGG 0.552251 8:-90633376 None:intergenic
AGTCGGTCAGAGAAGGAGGT+TGG 0.561135 8:-90633369 None:intergenic
TACCAGTGCTGCAATGATGA+TGG 0.562350 8:+90633215 MsG0880047772.01.T01:CDS
CTCCTTCTCTGACCGACTGT+GGG 0.564074 8:+90633374 MsG0880047772.01.T01:CDS
CTAGTACAATATGCAGCTGC+GGG 0.565924 8:+90633099 MsG0880047772.01.T01:CDS
ATCCTTCTTCATCTTCTGAA+CGG 0.566170 8:-90633026 None:intergenic
TGGGGTGATTATGATCCTCA+GGG 0.566445 8:-90633410 None:intergenic
GAACGGTAATTAGTAGGAGG+AGG 0.572179 8:-90633009 None:intergenic
GGATTTGGATGATTGAGATG+TGG 0.580035 8:-90633297 None:intergenic
AATTAGTGATTACTTGAAGG+AGG 0.582479 8:-90633447 None:intergenic
CTGTTAGTCTCCGCTTGTGG+TGG 0.588546 8:+90633129 MsG0880047772.01.T01:CDS
AACATAGTTAACAAACTGGA+AGG 0.592045 8:+90632922 MsG0880047772.01.T01:CDS
TCTCTGTTAGTCTCCGCTTG+TGG 0.618457 8:+90633126 MsG0880047772.01.T01:CDS
TTTCCCAGTCTCAGCTTCAG+CGG 0.622682 8:+90633164 MsG0880047772.01.T01:CDS
TCTGAACGGTAATTAGTAGG+AGG 0.623921 8:-90633012 None:intergenic
ACTAGTACAATATGCAGCTG+CGG 0.627166 8:+90633098 MsG0880047772.01.T01:CDS
AGCAGCAGCAACATCAACCT+AGG 0.647554 8:+90633073 MsG0880047772.01.T01:CDS
TTGGATTCCAGTCCCACAGT+CGG 0.664258 8:-90633386 None:intergenic
GTACTTGGAAAGCCCAAGAG+TGG 0.699274 8:-90632977 None:intergenic
GGACTGGAATCCAATCCCTG+AGG 0.753349 8:+90633395 MsG0880047772.01.T01:CDS
GAGGTGTTGTACTACTGTTG+GGG 0.769681 8:-90633428 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AACATAGTTAACAAACTGGA+AGG + Chr8:90632922-90632941 MsG0880047772.01.T01:CDS 30.0%
AGCAATGTAGACAAATGATT+GGG - Chr8:90633321-90633340 None:intergenic 30.0%
GATCAACATAGTTAACAAAC+TGG + Chr8:90632918-90632937 MsG0880047772.01.T01:CDS 30.0%
TATGTTGATCATCAAGTCTT+TGG - Chr8:90632908-90632927 None:intergenic 30.0%
!! GGAAATTGATTTGGTAACAT+TGG - Chr8:90633351-90633370 None:intergenic 30.0%
AAGGAGGTTGGAAATTGATT+TGG - Chr8:90633360-90633379 None:intergenic 35.0%
ATCCTTCTTCATCTTCTGAA+CGG - Chr8:90633029-90633048 None:intergenic 35.0%
CAGCAATGTAGACAAATGAT+TGG - Chr8:90633322-90633341 None:intergenic 35.0%
GATTTGGATGATTGAGATGT+GGG - Chr8:90633299-90633318 None:intergenic 35.0%
GTAGACAAATGATTGGGATT+TGG - Chr8:90633315-90633334 None:intergenic 35.0%
TCTTCTGAACGGTAATTAGT+AGG - Chr8:90633018-90633037 None:intergenic 35.0%
!! TGATGATGGATGATCTCAAT+AGG + Chr8:90633229-90633248 MsG0880047772.01.T01:CDS 35.0%
ACTAGTACAATATGCAGCTG+CGG + Chr8:90633098-90633117 MsG0880047772.01.T01:CDS 40.0%
ATCATGTGACAACCACTCTT+GGG + Chr8:90632965-90632984 MsG0880047772.01.T01:CDS 40.0%
GCATATTGTACTAGTTGCCT+AGG - Chr8:90633093-90633112 None:intergenic 40.0%
GGATTTGGATGATTGAGATG+TGG - Chr8:90633300-90633319 None:intergenic 40.0%
TACCGTTCAGAAGATGAAGA+AGG + Chr8:90633024-90633043 MsG0880047772.01.T01:CDS 40.0%
TCTGAACGGTAATTAGTAGG+AGG - Chr8:90633015-90633034 None:intergenic 40.0%
TGATTATGATCCTCAGGGAT+TGG - Chr8:90633408-90633427 None:intergenic 40.0%
ATCCATCATCATTGCAGCAC+TGG - Chr8:90633220-90633239 None:intergenic 45.0%
CTAGTACAATATGCAGCTGC+GGG + Chr8:90633099-90633118 MsG0880047772.01.T01:CDS 45.0%
GAACGGTAATTAGTAGGAGG+AGG - Chr8:90633012-90633031 None:intergenic 45.0%
GATCATGTGACAACCACTCT+TGG + Chr8:90632964-90632983 MsG0880047772.01.T01:CDS 45.0%
TACCAGTGCTGCAATGATGA+TGG + Chr8:90633215-90633234 MsG0880047772.01.T01:CDS 45.0%
TGAAGCTGAGACTGGGAAAA+TGG - Chr8:90633163-90633182 None:intergenic 45.0%
TGGGGTGATTATGATCCTCA+GGG - Chr8:90633413-90633432 None:intergenic 45.0%
TTGGGGTGATTATGATCCTC+AGG - Chr8:90633414-90633433 None:intergenic 45.0%
!! AGGAGGAGTATTGCTGTACT+TGG - Chr8:90632995-90633014 None:intergenic 45.0%
!! AGGAGGTGTTGTACTACTGT+TGG - Chr8:90633433-90633452 None:intergenic 45.0%
!! GAGGTGTTGTACTACTGTTG+GGG - Chr8:90633431-90633450 None:intergenic 45.0%
!! GGAGGTGTTGTACTACTGTT+GGG - Chr8:90633432-90633451 None:intergenic 45.0%
AGCAGCAGCAACATCAACCT+AGG + Chr8:90633073-90633092 MsG0880047772.01.T01:CDS 50.0%
GTACTTGGAAAGCCCAAGAG+TGG - Chr8:90632980-90632999 None:intergenic 50.0%
TTGGATTCCAGTCCCACAGT+CGG - Chr8:90633389-90633408 None:intergenic 50.0%
TTTCCCAGTCTCAGCTTCAG+CGG + Chr8:90633164-90633183 MsG0880047772.01.T01:CDS 50.0%
! TCTCTGTTAGTCTCCGCTTG+TGG + Chr8:90633126-90633145 MsG0880047772.01.T01:CDS 50.0%
!! TGCAGCACTGGTAGCAGTAT+TGG - Chr8:90633208-90633227 None:intergenic 50.0%
AGTCGGTCAGAGAAGGAGGT+TGG - Chr8:90633372-90633391 None:intergenic 55.0%
CTCCTTCTCTGACCGACTGT+GGG + Chr8:90633374-90633393 MsG0880047772.01.T01:CDS 55.0%
GGACTGGAATCCAATCCCTG+AGG + Chr8:90633395-90633414 MsG0880047772.01.T01:CDS 55.0%
TTAGTCTCCGCTTGTGGTGG+CGG + Chr8:90633132-90633151 MsG0880047772.01.T01:CDS 55.0%
! CTGTTAGTCTCCGCTTGTGG+TGG + Chr8:90633129-90633148 MsG0880047772.01.T01:CDS 55.0%
CCACAGTCGGTCAGAGAAGG+AGG - Chr8:90633376-90633395 None:intergenic 60.0%
CCTCCTTCTCTGACCGACTG+TGG + Chr8:90633373-90633392 MsG0880047772.01.T01:CDS 60.0%
CTGCCGCTGAAGCTGAGACT+GGG - Chr8:90633170-90633189 None:intergenic 60.0%
GTCCCACAGTCGGTCAGAGA+AGG - Chr8:90633379-90633398 None:intergenic 60.0%
TCTCTGACCGACTGTGGGAC+TGG + Chr8:90633379-90633398 MsG0880047772.01.T01:CDS 60.0%
GCTGCCGCTGAAGCTGAGAC+TGG - Chr8:90633171-90633190 None:intergenic 65.0%
GGCGCTGCCGCCACCACAAG+CGG - Chr8:90633142-90633161 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr8 gene 90632883 90633467 90632883 ID=MsG0880047772.01;Name=MsG0880047772.01
Chr8 mRNA 90632883 90633467 90632883 ID=MsG0880047772.01.T01;Parent=MsG0880047772.01;Name=MsG0880047772.01.T01;_AED=0.32;_eAED=0.32;_QI=0|-1|0|1|-1|1|1|0|194
Chr8 exon 90632883 90633467 90632883 ID=MsG0880047772.01.T01:exon:16168;Parent=MsG0880047772.01.T01
Chr8 CDS 90632883 90633467 90632883 ID=MsG0880047772.01.T01:cds;Parent=MsG0880047772.01.T01
Gene Sequence

>MsG0880047772.01.T01

ATGGGATTGTTGTTGGACATCACCAAAGACTTGATGATCAACATAGTTAACAAACTGGAAGGAGCAGTAGCGCGTAATTCAGATCATGTGACAACCACTCTTGGGCTTTCCAAGTACAGCAATACTCCTCCTCCTACTAATTACCGTTCAGAAGATGAAGAAGGATTGATGATGATGCAGCAGCAGCAGCAGCAGCAGCAACATCAACCTAGGCAACTAGTACAATATGCAGCTGCGGGAAACTCTCTGTTAGTCTCCGCTTGTGGTGGCGGCAGCGCCATTTTCCCAGTCTCAGCTTCAGCGGCAGCAGCAGTGAGCTGTTCCAATACTGCTACCAGTGCTGCAATGATGATGGATGATCTCAATAGGCTTGTAAGTTATCAACAACAGCAATATTGTTACAATGTTCAAAACCCACATCTCAATCATCCAAATCCCAATCATTTGTCTACATTGCTGATGCAACCAATGTTACCAAATCAATTTCCAACCTCCTTCTCTGACCGACTGTGGGACTGGAATCCAATCCCTGAGGATCATAATCACCCCAACAGTAGTACAACACCTCCTTCAAGTAATCACTAA

Protein sequence

>MsG0880047772.01.T01

MGLLLDITKDLMINIVNKLEGAVARNSDHVTTTLGLSKYSNTPPPTNYRSEDEEGLMMMQQQQQQQQHQPRQLVQYAAAGNSLLVSACGGGSAIFPVSASAAAAVSCSNTATSAAMMMDDLNRLVSYQQQQYCYNVQNPHLNHPNPNHLSTLLMQPMLPNQFPTSFSDRLWDWNPIPEDHNHPNSSTTPPSSNH*