AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0000040


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0000040 MtrunA17_Chr3g0093001 100.000 92 0 0 1 92 1 92 2.70e-60 178
Msa0000040 MtrunA17_Chr7g0217141 72.340 94 23 2 1 92 1 93 7.13e-38 122
Msa0000040 MtrunA17_Chr5g0416431 72.727 77 19 1 17 91 17 93 2.10e-33 110
Msa0000040 MtrunA17_Chr6g0453041 73.077 78 19 1 17 92 15 92 1.25e-32 108
Msa0000040 MtrunA17_Chr8g0362131 66.667 84 25 2 10 91 10 92 2.86e-31 105
Msa0000040 MtrunA17_Chr7g0237771 48.611 72 37 0 8 79 3 74 2.30e-11 54.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0000040 AT3G47710.1 73.118 93 23 2 1 92 1 92 2.77e-39 125
Msa0000040 AT1G74500.1 77.922 77 16 1 17 92 17 93 9.83e-36 117
Msa0000040 AT1G26945.1 79.730 74 13 1 19 90 19 92 1.55e-34 114
Msa0000040 AT3G28857.1 65.957 94 27 3 1 91 1 92 1.51e-32 108
Msa0000040 AT5G39860.1 69.231 78 21 2 17 91 15 92 1.01e-29 102
Msa0000040 AT5G15160.1 66.667 75 22 2 19 90 18 92 2.58e-27 95.9

Find 17 sgRNAs with CRISPR-Local

Find 138 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAGCTTCAAGGGTGTTGC+AGG 0.328305 1_1:+669978 Msa0000040:CDS
TGAGCGTTTGTCTGAGCTAT+TGG 0.392203 1_1:+670050 Msa0000040:CDS
TATATCAGGTTTCAGCTTCA+AGG 0.403696 1_1:+669968 Msa0000040:intron
ACTATATTAGAAACTTGAAC+AGG 0.414262 1_1:+670010 Msa0000040:CDS
GAGCTCCTATTGCGAATCTC+AGG 0.466230 1_1:-669103 None:intergenic
TTCTTCCTGAGATTCGCAAT+AGG 0.484861 1_1:+669098 Msa0000040:CDS
AGGAAGAAGTTGCTGCAGCT+TGG 0.493730 1_1:-669083 None:intergenic
ATATCAGGTTTCAGCTTCAA+GGG 0.502020 1_1:+669969 Msa0000040:intron
CTAGCAGGAGGTCGCGGTCA+CGG 0.548676 1_1:+669008 Msa0000040:CDS
GCTCAGACAAACGCTCACTT+AGG 0.559789 1_1:-670044 None:intergenic
GCAGCTTGGAGACAAGATCA+TGG 0.563717 1_1:-669069 None:intergenic
AGATGTCTAGCAGGAGGTCG+CGG 0.564343 1_1:+669002 None:intergenic
TCGCAATAGGAGCTCTGACA+AGG 0.593557 1_1:+669111 Msa0000040:CDS
AAGATCATGGATCTGATCGT+CGG 0.643584 1_1:-669056 None:intergenic
TTATAAAGATGTCTAGCAGG+AGG 0.648667 1_1:+668996 None:intergenic
CTATATTAGAAACTTGAACA+GGG 0.660572 1_1:+670011 Msa0000040:CDS
CACGGCAAACAAGTAGCTCG+AGG 0.663330 1_1:+669026 Msa0000040:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGACTTTTATAAAAAATAA+TGG - chr1_1:669264-669283 None:intergenic 10.0%
!!! TTATTTTTTATAAAAGTCAT+TGG + chr1_1:669264-669283 Msa0000040:intron 10.0%
!! TGTATTAAAAATATAGTATA+TGG - chr1_1:669358-669377 None:intergenic 10.0%
!!! AAGTTTTTTTTTTTTTTTCT+TGG - chr1_1:669677-669696 None:intergenic 10.0%
!! AAAAAAAAAAAAAACTTGAA+AGG + chr1_1:669680-669699 Msa0000040:intron 10.0%
!!! ATGACTTTTATAAAAAATAA+TGG - chr1_1:669264-669283 None:intergenic 10.0%
!!! TTATTTTTTATAAAAGTCAT+TGG + chr1_1:669264-669283 Msa0000040:intron 10.0%
!! TGTATTAAAAATATAGTATA+TGG - chr1_1:669358-669377 None:intergenic 10.0%
!!! AAGTTTTTTTTTTTTTTTCT+TGG - chr1_1:669677-669696 None:intergenic 10.0%
!! AAAAAAAAAAAAAACTTGAA+AGG + chr1_1:669680-669699 Msa0000040:intron 10.0%
!!! AAATTGAATTTTACTATATC+AGG + chr1_1:669954-669973 Msa0000040:intron 15.0%
!!! AAATTGAATTTTACTATATC+AGG + chr1_1:669954-669973 Msa0000040:intron 15.0%
! CTAGGAAACAAATAATATGT+AGG - chr1_1:669143-669162 None:intergenic 25.0%
!!! AGAAGAGATTAGTTTTATAG+CGG + chr1_1:669379-669398 Msa0000040:intron 25.0%
! GATAATGCATAATATATGCA+GGG + chr1_1:669584-669603 Msa0000040:intron 25.0%
! ATTTAAACCAACTTAATCCT+AGG + chr1_1:669768-669787 Msa0000040:intron 25.0%
! ACTATATTAGAAACTTGAAC+AGG + chr1_1:670010-670029 Msa0000040:CDS 25.0%
! CTATATTAGAAACTTGAACA+GGG + chr1_1:670011-670030 Msa0000040:CDS 25.0%
! CTAGGAAACAAATAATATGT+AGG - chr1_1:669143-669162 None:intergenic 25.0%
!!! AGAAGAGATTAGTTTTATAG+CGG + chr1_1:669379-669398 Msa0000040:intron 25.0%
! GATAATGCATAATATATGCA+GGG + chr1_1:669584-669603 Msa0000040:intron 25.0%
! ATTTAAACCAACTTAATCCT+AGG + chr1_1:669768-669787 Msa0000040:intron 25.0%
! ACTATATTAGAAACTTGAAC+AGG + chr1_1:670010-670029 Msa0000040:CDS 25.0%
! CTATATTAGAAACTTGAACA+GGG + chr1_1:670011-670030 Msa0000040:CDS 25.0%
!! CTAGTCTTTTGATTAAGACT+AGG - chr1_1:669161-669180 None:intergenic 30.0%
GGATAATGCATAATATATGC+AGG + chr1_1:669583-669602 Msa0000040:intron 30.0%
ACCATACTTATTCACCTTTA+AGG + chr1_1:669628-669647 Msa0000040:intron 30.0%
ACCTTAAAGGTGAATAAGTA+TGG - chr1_1:669632-669651 None:intergenic 30.0%
TACACTAGAGTAAGTATAAG+AGG + chr1_1:669743-669762 Msa0000040:intron 30.0%
ACACTAGAGTAAGTATAAGA+GGG + chr1_1:669744-669763 Msa0000040:intron 30.0%
CAAGAAAAAGGAACAACAAT+AGG + chr1_1:669804-669823 Msa0000040:intron 30.0%
CATCAACAATTCTTGTAGAT+GGG - chr1_1:669879-669898 None:intergenic 30.0%
ACATCAACAATTCTTGTAGA+TGG - chr1_1:669880-669899 None:intergenic 30.0%
!! CTAGTCTTTTGATTAAGACT+AGG - chr1_1:669161-669180 None:intergenic 30.0%
GGATAATGCATAATATATGC+AGG + chr1_1:669583-669602 Msa0000040:intron 30.0%
ACCATACTTATTCACCTTTA+AGG + chr1_1:669628-669647 Msa0000040:intron 30.0%
ACCTTAAAGGTGAATAAGTA+TGG - chr1_1:669632-669651 None:intergenic 30.0%
TACACTAGAGTAAGTATAAG+AGG + chr1_1:669743-669762 Msa0000040:intron 30.0%
ACACTAGAGTAAGTATAAGA+GGG + chr1_1:669744-669763 Msa0000040:intron 30.0%
CAAGAAAAAGGAACAACAAT+AGG + chr1_1:669804-669823 Msa0000040:intron 30.0%
CATCAACAATTCTTGTAGAT+GGG - chr1_1:669879-669898 None:intergenic 30.0%
ACATCAACAATTCTTGTAGA+TGG - chr1_1:669880-669899 None:intergenic 30.0%
GAGATACCCTATTAATCTAC+CGG + chr1_1:669423-669442 Msa0000040:intron 35.0%
TGAGCTTAGTTCATTTGGTA+AGG + chr1_1:669561-669580 Msa0000040:intron 35.0%
GAGCTTAGTTCATTTGGTAA+GGG + chr1_1:669562-669581 Msa0000040:intron 35.0%
!! TTATTCACCTTTAAGGTCTC+TGG + chr1_1:669635-669654 Msa0000040:intron 35.0%
! TTTCTTGGTCAATTAGCCTA+GGG - chr1_1:669662-669681 None:intergenic 35.0%
!! TTTTCTTGGTCAATTAGCCT+AGG - chr1_1:669663-669682 None:intergenic 35.0%
AATAGGCCTAACAAATACGA+GGG + chr1_1:669821-669840 Msa0000040:intron 35.0%
TTAATCCCCTCGTATTTGTT+AGG - chr1_1:669830-669849 None:intergenic 35.0%
TCTTGTAGATGGGAAAAAAC+TGG - chr1_1:669869-669888 None:intergenic 35.0%
TATATCAGGTTTCAGCTTCA+AGG + chr1_1:669968-669987 Msa0000040:intron 35.0%
ATATCAGGTTTCAGCTTCAA+GGG + chr1_1:669969-669988 Msa0000040:intron 35.0%
GAGATACCCTATTAATCTAC+CGG + chr1_1:669423-669442 Msa0000040:intron 35.0%
TGAGCTTAGTTCATTTGGTA+AGG + chr1_1:669561-669580 Msa0000040:intron 35.0%
GAGCTTAGTTCATTTGGTAA+GGG + chr1_1:669562-669581 Msa0000040:intron 35.0%
!! TTATTCACCTTTAAGGTCTC+TGG + chr1_1:669635-669654 Msa0000040:intron 35.0%
! TTTCTTGGTCAATTAGCCTA+GGG - chr1_1:669662-669681 None:intergenic 35.0%
!! TTTTCTTGGTCAATTAGCCT+AGG - chr1_1:669663-669682 None:intergenic 35.0%
AATAGGCCTAACAAATACGA+GGG + chr1_1:669821-669840 Msa0000040:intron 35.0%
TTAATCCCCTCGTATTTGTT+AGG - chr1_1:669830-669849 None:intergenic 35.0%
TCTTGTAGATGGGAAAAAAC+TGG - chr1_1:669869-669888 None:intergenic 35.0%
TATATCAGGTTTCAGCTTCA+AGG + chr1_1:669968-669987 Msa0000040:intron 35.0%
ATATCAGGTTTCAGCTTCAA+GGG + chr1_1:669969-669988 Msa0000040:intron 35.0%
AAGATCATGGATCTGATCGT+CGG - chr1_1:669059-669078 None:intergenic 40.0%
TTCTTCCTGAGATTCGCAAT+AGG + chr1_1:669098-669117 Msa0000040:CDS 40.0%
GACTCTCCGGTAGATTAATA+GGG - chr1_1:669432-669451 None:intergenic 40.0%
TGACTCTCCGGTAGATTAAT+AGG - chr1_1:669433-669452 None:intergenic 40.0%
!! GAGCTGTTTTGTGAGCTTTT+AGG - chr1_1:669475-669494 None:intergenic 40.0%
AGCTCAGAAGGACTTCTTTA+CGG - chr1_1:669547-669566 None:intergenic 40.0%
CCAAATGAACTAAGCTCAGA+AGG - chr1_1:669559-669578 None:intergenic 40.0%
CCTTCTGAGCTTAGTTCATT+TGG + chr1_1:669556-669575 Msa0000040:intron 40.0%
TGCATAATATATGCAGGGAC+CGG + chr1_1:669589-669608 Msa0000040:intron 40.0%
GTGAATAAGTATGGTGTCTG+GGG - chr1_1:669623-669642 None:intergenic 40.0%
GGTGAATAAGTATGGTGTCT+GGG - chr1_1:669624-669643 None:intergenic 40.0%
AGGTGAATAAGTATGGTGTC+TGG - chr1_1:669625-669644 None:intergenic 40.0%
! TTCTTGGTCAATTAGCCTAG+GGG - chr1_1:669661-669680 None:intergenic 40.0%
!! CCTTTTTCTTGTTATGTCCC+TGG - chr1_1:669795-669814 None:intergenic 40.0%
CCAGGGACATAACAAGAAAA+AGG + chr1_1:669792-669811 Msa0000040:intron 40.0%
CAATAGGCCTAACAAATACG+AGG + chr1_1:669820-669839 Msa0000040:intron 40.0%
ATAGGCCTAACAAATACGAG+GGG + chr1_1:669822-669841 Msa0000040:intron 40.0%
AAGATCATGGATCTGATCGT+CGG - chr1_1:669059-669078 None:intergenic 40.0%
TTCTTCCTGAGATTCGCAAT+AGG + chr1_1:669098-669117 Msa0000040:CDS 40.0%
GACTCTCCGGTAGATTAATA+GGG - chr1_1:669432-669451 None:intergenic 40.0%
TGACTCTCCGGTAGATTAAT+AGG - chr1_1:669433-669452 None:intergenic 40.0%
!! GAGCTGTTTTGTGAGCTTTT+AGG - chr1_1:669475-669494 None:intergenic 40.0%
AGCTCAGAAGGACTTCTTTA+CGG - chr1_1:669547-669566 None:intergenic 40.0%
CCAAATGAACTAAGCTCAGA+AGG - chr1_1:669559-669578 None:intergenic 40.0%
CCTTCTGAGCTTAGTTCATT+TGG + chr1_1:669556-669575 Msa0000040:intron 40.0%
TGCATAATATATGCAGGGAC+CGG + chr1_1:669589-669608 Msa0000040:intron 40.0%
GTGAATAAGTATGGTGTCTG+GGG - chr1_1:669623-669642 None:intergenic 40.0%
GGTGAATAAGTATGGTGTCT+GGG - chr1_1:669624-669643 None:intergenic 40.0%
AGGTGAATAAGTATGGTGTC+TGG - chr1_1:669625-669644 None:intergenic 40.0%
! TTCTTGGTCAATTAGCCTAG+GGG - chr1_1:669661-669680 None:intergenic 40.0%
!! CCTTTTTCTTGTTATGTCCC+TGG - chr1_1:669795-669814 None:intergenic 40.0%
CCAGGGACATAACAAGAAAA+AGG + chr1_1:669792-669811 Msa0000040:intron 40.0%
CAATAGGCCTAACAAATACG+AGG + chr1_1:669820-669839 Msa0000040:intron 40.0%
ATAGGCCTAACAAATACGAG+GGG + chr1_1:669822-669841 Msa0000040:intron 40.0%
ACCAACTTAATCCTAGGTCC+AGG + chr1_1:669774-669793 Msa0000040:intron 45.0%
CCAACTTAATCCTAGGTCCA+GGG + chr1_1:669775-669794 Msa0000040:intron 45.0%
TGAGCGTTTGTCTGAGCTAT+TGG + chr1_1:670050-670069 Msa0000040:CDS 45.0%
ACCAACTTAATCCTAGGTCC+AGG + chr1_1:669774-669793 Msa0000040:intron 45.0%
CCAACTTAATCCTAGGTCCA+GGG + chr1_1:669775-669794 Msa0000040:intron 45.0%
TGAGCGTTTGTCTGAGCTAT+TGG + chr1_1:670050-670069 Msa0000040:CDS 45.0%
GCAGCTTGGAGACAAGATCA+TGG - chr1_1:669072-669091 None:intergenic 50.0%
AGGAAGAAGTTGCTGCAGCT+TGG - chr1_1:669086-669105 None:intergenic 50.0%
GAGCTCCTATTGCGAATCTC+AGG - chr1_1:669106-669125 None:intergenic 50.0%
! TCGCAATAGGAGCTCTGACA+AGG + chr1_1:669111-669130 Msa0000040:CDS 50.0%
! CATGGTTAGAGCTGACTCTC+CGG - chr1_1:669445-669464 None:intergenic 50.0%
! AGAGTCAGCTCTAACCATGG+TGG + chr1_1:669446-669465 Msa0000040:intron 50.0%
!! GAGCTTTTAGGAGTCCACCA+TGG - chr1_1:669463-669482 None:intergenic 50.0%
CCCTGGACCTAGGATTAAGT+TGG - chr1_1:669778-669797 None:intergenic 50.0%
! CTTGTTATGTCCCTGGACCT+AGG - chr1_1:669788-669807 None:intergenic 50.0%
!! TTCAGCTTCAAGGGTGTTGC+AGG + chr1_1:669978-669997 Msa0000040:CDS 50.0%
GCTCAGACAAACGCTCACTT+AGG - chr1_1:670047-670066 None:intergenic 50.0%
GCAGCTTGGAGACAAGATCA+TGG - chr1_1:669072-669091 None:intergenic 50.0%
AGGAAGAAGTTGCTGCAGCT+TGG - chr1_1:669086-669105 None:intergenic 50.0%
GAGCTCCTATTGCGAATCTC+AGG - chr1_1:669106-669125 None:intergenic 50.0%
! TCGCAATAGGAGCTCTGACA+AGG + chr1_1:669111-669130 Msa0000040:CDS 50.0%
! CATGGTTAGAGCTGACTCTC+CGG - chr1_1:669445-669464 None:intergenic 50.0%
! AGAGTCAGCTCTAACCATGG+TGG + chr1_1:669446-669465 Msa0000040:intron 50.0%
!! GAGCTTTTAGGAGTCCACCA+TGG - chr1_1:669463-669482 None:intergenic 50.0%
CCCTGGACCTAGGATTAAGT+TGG - chr1_1:669778-669797 None:intergenic 50.0%
! CTTGTTATGTCCCTGGACCT+AGG - chr1_1:669788-669807 None:intergenic 50.0%
!! TTCAGCTTCAAGGGTGTTGC+AGG + chr1_1:669978-669997 Msa0000040:CDS 50.0%
GCTCAGACAAACGCTCACTT+AGG - chr1_1:670047-670066 None:intergenic 50.0%
CACGGCAAACAAGTAGCTCG+AGG + chr1_1:669026-669045 Msa0000040:CDS 55.0%
! CGGAGAGTCAGCTCTAACCA+TGG + chr1_1:669443-669462 Msa0000040:intron 55.0%
CTAGGGGCCAGAGACCTTAA+AGG - chr1_1:669645-669664 None:intergenic 55.0%
!! CTTTAAGGTCTCTGGCCCCT+AGG + chr1_1:669643-669662 Msa0000040:intron 55.0%
CACGGCAAACAAGTAGCTCG+AGG + chr1_1:669026-669045 Msa0000040:CDS 55.0%
! CGGAGAGTCAGCTCTAACCA+TGG + chr1_1:669443-669462 Msa0000040:intron 55.0%
CTAGGGGCCAGAGACCTTAA+AGG - chr1_1:669645-669664 None:intergenic 55.0%
!! CTTTAAGGTCTCTGGCCCCT+AGG + chr1_1:669643-669662 Msa0000040:intron 55.0%
! GGTGTCTGGGGTTCGAACTC+CGG - chr1_1:669611-669630 None:intergenic 60.0%
! GGTGTCTGGGGTTCGAACTC+CGG - chr1_1:669611-669630 None:intergenic 60.0%
CTAGCAGGAGGTCGCGGTCA+CGG + chr1_1:669008-669027 Msa0000040:CDS 65.0%
CTAGCAGGAGGTCGCGGTCA+CGG + chr1_1:669008-669027 Msa0000040:CDS 65.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 669004 670125 669004 ID=Msa0000040;Name=Msa0000040
chr1_1 mRNA 669004 670125 669004 ID=Msa0000040-mRNA-1;Parent=Msa0000040;Name=Msa0000040-mRNA-1;_AED=0.04;_eAED=0.05;_QI=0|0|0|1|1|1|2|0|92
chr1_1 exon 669004 669132 669004 ID=Msa0000040-mRNA-1:exon:4;Parent=Msa0000040-mRNA-1
chr1_1 exon 669976 670125 669976 ID=Msa0000040-mRNA-1:exon:5;Parent=Msa0000040-mRNA-1
chr1_1 CDS 669004 669132 669004 ID=Msa0000040-mRNA-1:cds;Parent=Msa0000040-mRNA-1
chr1_1 CDS 669976 670125 669976 ID=Msa0000040-mRNA-1:cds;Parent=Msa0000040-mRNA-1
Gene Sequence

>Msa0000040

ATGTCTAGCAGGAGGTCGCGGTCACGGCAAACAAGTAGCTCGAGGAATATCACCGACGATCAGATCCATGATCTTGTCTCCAAGCTGCAGCAACTTCTTCCTGAGATTCGCAATAGGAGCTCTGACAAGGTTTCAGCTTCAAGGGTGTTGCAGGAGACTTGCAACTATATTAGAAACTTGAACAGGGAAGTTGACGACCTAAGTGAGCGTTTGTCTGAGCTATTGGCTACGACAGACACAGCACAAGCAGCAATAATTAGAAATTTACTTATGCAATAG

Protein sequence

>Msa0000040

MSSRRSRSRQTSSSRNITDDQIHDLVSKLQQLLPEIRNRSSDKVSASRVLQETCNYIRNLNREVDDLSERLSELLATTDTAQAAIIRNLLMQ*