AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0449480


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0449480 MtrunA17_Chr3g0093001 100.000 92 0 0 1 92 1 92 2.70e-60 178
Msa0449480 MtrunA17_Chr7g0217141 72.340 94 23 2 1 92 1 93 7.13e-38 122
Msa0449480 MtrunA17_Chr5g0416431 72.727 77 19 1 17 91 17 93 2.10e-33 110
Msa0449480 MtrunA17_Chr6g0453041 73.077 78 19 1 17 92 15 92 1.25e-32 108
Msa0449480 MtrunA17_Chr8g0362131 66.667 84 25 2 10 91 10 92 2.86e-31 105
Msa0449480 MtrunA17_Chr7g0237771 48.611 72 37 0 8 79 3 74 2.30e-11 54.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0449480 AT3G47710.1 73.118 93 23 2 1 92 1 92 2.77e-39 125
Msa0449480 AT1G74500.1 77.922 77 16 1 17 92 17 93 9.83e-36 117
Msa0449480 AT1G26945.1 79.730 74 13 1 19 90 19 92 1.55e-34 114
Msa0449480 AT3G28857.1 65.957 94 27 3 1 91 1 92 1.51e-32 108
Msa0449480 AT5G39860.1 69.231 78 21 2 17 91 15 92 1.01e-29 102
Msa0449480 AT5G15160.1 66.667 75 22 2 19 90 18 92 2.58e-27 95.9

Find 15 sgRNAs with CRISPR-Local

Find 134 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAGCTTCGAGGGTGTTGC+AGG 0.348972 3_3:-33183377 Msa0449480:CDS
TGAGCGTTTGTCTGAGCTAT+TGG 0.392203 3_3:-33183305 Msa0449480:CDS
GAGCTCCTATTGCGAATCTC+AGG 0.466230 3_3:+33184246 None:intergenic
TATATCAGGTTTCAGCTTCG+AGG 0.481740 3_3:-33183387 Msa0449480:intron
TTCTTCCTGAGATTCGCAAT+AGG 0.484861 3_3:-33184251 Msa0449480:CDS
AGGAAGAAGTTGCTGCAGCT+TGG 0.493730 3_3:+33184266 None:intergenic
CTAGCAGGAGGTCGCGGTCA+CGG 0.548676 3_3:-33184341 Msa0449480:CDS
ATATCAGGTTTCAGCTTCGA+GGG 0.550730 3_3:-33183386 Msa0449480:intron
GCTCAGACAAACGCTCACTT+AGG 0.559789 3_3:+33183311 None:intergenic
GCAGCTTGGAGACAAGATCA+TGG 0.563717 3_3:+33184280 None:intergenic
AGATGTCTAGCAGGAGGTCG+CGG 0.564343 3_3:-33184347 Msa0449480:CDS
TCGCAATAGGAGCTCTGACA+AGG 0.593557 3_3:-33184238 Msa0449480:intron
AAGATCATGGATCTGATCGT+CGG 0.643584 3_3:+33184293 None:intergenic
TTATAAAGATGTCTAGCAGG+AGG 0.648667 3_3:-33184353 None:intergenic
CACGGCAAACAAGTAGCTCG+AGG 0.663330 3_3:-33184323 Msa0449480:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGACTTTTATAAAAAATAA+TGG + chr3_3:33183512-33183531 None:intergenic 10.0%
!!! TTATTTTTTATAAAAGTCAT+TGG - chr3_3:33183512-33183531 Msa0449480:intron 10.0%
!! TGTATTAAAAATATAGTATA+TGG + chr3_3:33183606-33183625 None:intergenic 10.0%
!!! AAGTTTTTTTTTTTTTTTCT+TGG + chr3_3:33183925-33183944 None:intergenic 10.0%
!! AAAAAAAAAAAAAACTTGAA+AGG - chr3_3:33183928-33183947 Msa0449480:intron 10.0%
!!! ATGACTTTTATAAAAAATAA+TGG + chr3_3:33183512-33183531 None:intergenic 10.0%
!!! TTATTTTTTATAAAAGTCAT+TGG - chr3_3:33183512-33183531 Msa0449480:intron 10.0%
!! TGTATTAAAAATATAGTATA+TGG + chr3_3:33183606-33183625 None:intergenic 10.0%
!!! AAGTTTTTTTTTTTTTTTCT+TGG + chr3_3:33183925-33183944 None:intergenic 10.0%
!! AAAAAAAAAAAAAACTTGAA+AGG - chr3_3:33183928-33183947 Msa0449480:intron 10.0%
!!! AAATTGAATTTTACTATATC+AGG - chr3_3:33184196-33184215 Msa0449480:intron 15.0%
!!! AAATTGAATTTTACTATATC+AGG - chr3_3:33184196-33184215 Msa0449480:intron 15.0%
! CTAGGAAACAAATAATATGT+AGG + chr3_3:33183391-33183410 None:intergenic 25.0%
! ATATAGTATATGGTAGTTAC+AGG + chr3_3:33183596-33183615 None:intergenic 25.0%
!!! AGAAGAGATTAGTTTTATAG+CGG - chr3_3:33183627-33183646 Msa0449480:intron 25.0%
! ATTTAAACCAACTTAATCCT+AGG - chr3_3:33184016-33184035 Msa0449480:intron 25.0%
! TGAAAACAATTCTTGTAGAT+GGG + chr3_3:33184127-33184146 None:intergenic 25.0%
! TTGAAAACAATTCTTGTAGA+TGG + chr3_3:33184128-33184147 None:intergenic 25.0%
! CTAGGAAACAAATAATATGT+AGG + chr3_3:33183391-33183410 None:intergenic 25.0%
! ATATAGTATATGGTAGTTAC+AGG + chr3_3:33183596-33183615 None:intergenic 25.0%
!!! AGAAGAGATTAGTTTTATAG+CGG - chr3_3:33183627-33183646 Msa0449480:intron 25.0%
! ATTTAAACCAACTTAATCCT+AGG - chr3_3:33184016-33184035 Msa0449480:intron 25.0%
! TGAAAACAATTCTTGTAGAT+GGG + chr3_3:33184127-33184146 None:intergenic 25.0%
! TTGAAAACAATTCTTGTAGA+TGG + chr3_3:33184128-33184147 None:intergenic 25.0%
!! CTAGTCTTTTGATTAAGACT+AGG + chr3_3:33183409-33183428 None:intergenic 30.0%
GATAATGCATACTATATGCA+GGG - chr3_3:33183832-33183851 Msa0449480:intron 30.0%
ACCATACTTATTCACCTTTA+AGG - chr3_3:33183876-33183895 Msa0449480:intron 30.0%
ACCTTAAAGGTGAATAAGTA+TGG + chr3_3:33183880-33183899 None:intergenic 30.0%
TACACTAGAGTAAGTATAAG+AGG - chr3_3:33183991-33184010 Msa0449480:intron 30.0%
ACACTAGAGTAAGTATAAGA+GGG - chr3_3:33183992-33184011 Msa0449480:intron 30.0%
!! CTAGTCTTTTGATTAAGACT+AGG + chr3_3:33183409-33183428 None:intergenic 30.0%
GATAATGCATACTATATGCA+GGG - chr3_3:33183832-33183851 Msa0449480:intron 30.0%
ACCATACTTATTCACCTTTA+AGG - chr3_3:33183876-33183895 Msa0449480:intron 30.0%
ACCTTAAAGGTGAATAAGTA+TGG + chr3_3:33183880-33183899 None:intergenic 30.0%
TACACTAGAGTAAGTATAAG+AGG - chr3_3:33183991-33184010 Msa0449480:intron 30.0%
ACACTAGAGTAAGTATAAGA+GGG - chr3_3:33183992-33184011 Msa0449480:intron 30.0%
GAGATACCCTATTAATCTAC+CGG - chr3_3:33183671-33183690 Msa0449480:intron 35.0%
TGAGCTTAGTTCATTTGGTA+AGG - chr3_3:33183809-33183828 Msa0449480:intron 35.0%
GAGCTTAGTTCATTTGGTAA+GGG - chr3_3:33183810-33183829 Msa0449480:intron 35.0%
GGATAATGCATACTATATGC+AGG - chr3_3:33183831-33183850 Msa0449480:intron 35.0%
!! TTATTCACCTTTAAGGTCTC+TGG - chr3_3:33183883-33183902 Msa0449480:intron 35.0%
! TTTCTTGGTCAATTAGCCTA+GGG + chr3_3:33183910-33183929 None:intergenic 35.0%
!! TTTTCTTGGTCAATTAGCCT+AGG + chr3_3:33183911-33183930 None:intergenic 35.0%
CAAGAAAAAGGAACAACAAC+AGG - chr3_3:33184052-33184071 Msa0449480:intron 35.0%
TTAATCCCCTCGTATTTGTT+AGG + chr3_3:33184078-33184097 None:intergenic 35.0%
TCTTGTAGATGGGAAAAAAC+TGG + chr3_3:33184117-33184136 None:intergenic 35.0%
GAGATACCCTATTAATCTAC+CGG - chr3_3:33183671-33183690 Msa0449480:intron 35.0%
TGAGCTTAGTTCATTTGGTA+AGG - chr3_3:33183809-33183828 Msa0449480:intron 35.0%
GAGCTTAGTTCATTTGGTAA+GGG - chr3_3:33183810-33183829 Msa0449480:intron 35.0%
GGATAATGCATACTATATGC+AGG - chr3_3:33183831-33183850 Msa0449480:intron 35.0%
!! TTATTCACCTTTAAGGTCTC+TGG - chr3_3:33183883-33183902 Msa0449480:intron 35.0%
! TTTCTTGGTCAATTAGCCTA+GGG + chr3_3:33183910-33183929 None:intergenic 35.0%
!! TTTTCTTGGTCAATTAGCCT+AGG + chr3_3:33183911-33183930 None:intergenic 35.0%
CAAGAAAAAGGAACAACAAC+AGG - chr3_3:33184052-33184071 Msa0449480:intron 35.0%
TTAATCCCCTCGTATTTGTT+AGG + chr3_3:33184078-33184097 None:intergenic 35.0%
TCTTGTAGATGGGAAAAAAC+TGG + chr3_3:33184117-33184136 None:intergenic 35.0%
AAGATCATGGATCTGATCGT+CGG + chr3_3:33183307-33183326 None:intergenic 40.0%
TTCTTCCTGAGATTCGCAAT+AGG - chr3_3:33183346-33183365 Msa0449480:CDS 40.0%
GACTCTCCGGTAGATTAATA+GGG + chr3_3:33183680-33183699 None:intergenic 40.0%
TGACTCTCCGGTAGATTAAT+AGG + chr3_3:33183681-33183700 None:intergenic 40.0%
!! GAGCTGTTTTGTGAGCTTTT+AGG + chr3_3:33183723-33183742 None:intergenic 40.0%
AGCTCAGAAGGACTTCTTTA+CGG + chr3_3:33183795-33183814 None:intergenic 40.0%
CCAAATGAACTAAGCTCAGA+AGG + chr3_3:33183807-33183826 None:intergenic 40.0%
CCTTCTGAGCTTAGTTCATT+TGG - chr3_3:33183804-33183823 Msa0449480:intron 40.0%
GTGAATAAGTATGGTGTCTG+GGG + chr3_3:33183871-33183890 None:intergenic 40.0%
GGTGAATAAGTATGGTGTCT+GGG + chr3_3:33183872-33183891 None:intergenic 40.0%
AGGTGAATAAGTATGGTGTC+TGG + chr3_3:33183873-33183892 None:intergenic 40.0%
! TTCTTGGTCAATTAGCCTAG+GGG + chr3_3:33183909-33183928 None:intergenic 40.0%
!! CCTTTTTCTTGTTATGTCCC+TGG + chr3_3:33184043-33184062 None:intergenic 40.0%
CCAGGGACATAACAAGAAAA+AGG - chr3_3:33184040-33184059 Msa0449480:intron 40.0%
AACAGGCCTAACAAATACGA+GGG - chr3_3:33184069-33184088 Msa0449480:intron 40.0%
TATATCAGGTTTCAGCTTCG+AGG - chr3_3:33184210-33184229 Msa0449480:intron 40.0%
ATATCAGGTTTCAGCTTCGA+GGG - chr3_3:33184211-33184230 Msa0449480:intron 40.0%
AAGATCATGGATCTGATCGT+CGG + chr3_3:33183307-33183326 None:intergenic 40.0%
TTCTTCCTGAGATTCGCAAT+AGG - chr3_3:33183346-33183365 Msa0449480:CDS 40.0%
GACTCTCCGGTAGATTAATA+GGG + chr3_3:33183680-33183699 None:intergenic 40.0%
TGACTCTCCGGTAGATTAAT+AGG + chr3_3:33183681-33183700 None:intergenic 40.0%
!! GAGCTGTTTTGTGAGCTTTT+AGG + chr3_3:33183723-33183742 None:intergenic 40.0%
AGCTCAGAAGGACTTCTTTA+CGG + chr3_3:33183795-33183814 None:intergenic 40.0%
CCAAATGAACTAAGCTCAGA+AGG + chr3_3:33183807-33183826 None:intergenic 40.0%
CCTTCTGAGCTTAGTTCATT+TGG - chr3_3:33183804-33183823 Msa0449480:intron 40.0%
GTGAATAAGTATGGTGTCTG+GGG + chr3_3:33183871-33183890 None:intergenic 40.0%
GGTGAATAAGTATGGTGTCT+GGG + chr3_3:33183872-33183891 None:intergenic 40.0%
AGGTGAATAAGTATGGTGTC+TGG + chr3_3:33183873-33183892 None:intergenic 40.0%
! TTCTTGGTCAATTAGCCTAG+GGG + chr3_3:33183909-33183928 None:intergenic 40.0%
!! CCTTTTTCTTGTTATGTCCC+TGG + chr3_3:33184043-33184062 None:intergenic 40.0%
CCAGGGACATAACAAGAAAA+AGG - chr3_3:33184040-33184059 Msa0449480:intron 40.0%
AACAGGCCTAACAAATACGA+GGG - chr3_3:33184069-33184088 Msa0449480:intron 40.0%
TATATCAGGTTTCAGCTTCG+AGG - chr3_3:33184210-33184229 Msa0449480:intron 40.0%
ATATCAGGTTTCAGCTTCGA+GGG - chr3_3:33184211-33184230 Msa0449480:intron 40.0%
! CATAGTTAGAGCTGACTCTC+CGG + chr3_3:33183693-33183712 None:intergenic 45.0%
! AGAGTCAGCTCTAACTATGG+TGG - chr3_3:33183694-33183713 Msa0449480:intron 45.0%
TGCATACTATATGCAGGGAC+CGG - chr3_3:33183837-33183856 Msa0449480:intron 45.0%
ACCAACTTAATCCTAGGTCC+AGG - chr3_3:33184022-33184041 Msa0449480:intron 45.0%
CCAACTTAATCCTAGGTCCA+GGG - chr3_3:33184023-33184042 Msa0449480:intron 45.0%
CAACAGGCCTAACAAATACG+AGG - chr3_3:33184068-33184087 Msa0449480:intron 45.0%
ACAGGCCTAACAAATACGAG+GGG - chr3_3:33184070-33184089 Msa0449480:intron 45.0%
TGAGCGTTTGTCTGAGCTAT+TGG - chr3_3:33184292-33184311 Msa0449480:CDS 45.0%
! CATAGTTAGAGCTGACTCTC+CGG + chr3_3:33183693-33183712 None:intergenic 45.0%
! AGAGTCAGCTCTAACTATGG+TGG - chr3_3:33183694-33183713 Msa0449480:intron 45.0%
TGCATACTATATGCAGGGAC+CGG - chr3_3:33183837-33183856 Msa0449480:intron 45.0%
ACCAACTTAATCCTAGGTCC+AGG - chr3_3:33184022-33184041 Msa0449480:intron 45.0%
CCAACTTAATCCTAGGTCCA+GGG - chr3_3:33184023-33184042 Msa0449480:intron 45.0%
CAACAGGCCTAACAAATACG+AGG - chr3_3:33184068-33184087 Msa0449480:intron 45.0%
ACAGGCCTAACAAATACGAG+GGG - chr3_3:33184070-33184089 Msa0449480:intron 45.0%
TGAGCGTTTGTCTGAGCTAT+TGG - chr3_3:33184292-33184311 Msa0449480:CDS 45.0%
GCAGCTTGGAGACAAGATCA+TGG + chr3_3:33183320-33183339 None:intergenic 50.0%
AGGAAGAAGTTGCTGCAGCT+TGG + chr3_3:33183334-33183353 None:intergenic 50.0%
GAGCTCCTATTGCGAATCTC+AGG + chr3_3:33183354-33183373 None:intergenic 50.0%
! TCGCAATAGGAGCTCTGACA+AGG - chr3_3:33183359-33183378 Msa0449480:CDS 50.0%
! CGGAGAGTCAGCTCTAACTA+TGG - chr3_3:33183691-33183710 Msa0449480:intron 50.0%
CCCTGGACCTAGGATTAAGT+TGG + chr3_3:33184026-33184045 None:intergenic 50.0%
! CTTGTTATGTCCCTGGACCT+AGG + chr3_3:33184036-33184055 None:intergenic 50.0%
GCTCAGACAAACGCTCACTT+AGG + chr3_3:33184289-33184308 None:intergenic 50.0%
GCAGCTTGGAGACAAGATCA+TGG + chr3_3:33183320-33183339 None:intergenic 50.0%
AGGAAGAAGTTGCTGCAGCT+TGG + chr3_3:33183334-33183353 None:intergenic 50.0%
GAGCTCCTATTGCGAATCTC+AGG + chr3_3:33183354-33183373 None:intergenic 50.0%
! TCGCAATAGGAGCTCTGACA+AGG - chr3_3:33183359-33183378 Msa0449480:CDS 50.0%
! CGGAGAGTCAGCTCTAACTA+TGG - chr3_3:33183691-33183710 Msa0449480:intron 50.0%
CCCTGGACCTAGGATTAAGT+TGG + chr3_3:33184026-33184045 None:intergenic 50.0%
! CTTGTTATGTCCCTGGACCT+AGG + chr3_3:33184036-33184055 None:intergenic 50.0%
GCTCAGACAAACGCTCACTT+AGG + chr3_3:33184289-33184308 None:intergenic 50.0%
CACGGCAAACAAGTAGCTCG+AGG - chr3_3:33183274-33183293 Msa0449480:CDS 55.0%
CTAGGGGCCAGAGACCTTAA+AGG + chr3_3:33183893-33183912 None:intergenic 55.0%
!! CTTTAAGGTCTCTGGCCCCT+AGG - chr3_3:33183891-33183910 Msa0449480:intron 55.0%
!! TTCAGCTTCGAGGGTGTTGC+AGG - chr3_3:33184220-33184239 Msa0449480:intron 55.0%
CACGGCAAACAAGTAGCTCG+AGG - chr3_3:33183274-33183293 Msa0449480:CDS 55.0%
CTAGGGGCCAGAGACCTTAA+AGG + chr3_3:33183893-33183912 None:intergenic 55.0%
!! CTTTAAGGTCTCTGGCCCCT+AGG - chr3_3:33183891-33183910 Msa0449480:intron 55.0%
!! TTCAGCTTCGAGGGTGTTGC+AGG - chr3_3:33184220-33184239 Msa0449480:intron 55.0%
! GGTGTCTGGGGTTCGAACTC+CGG + chr3_3:33183859-33183878 None:intergenic 60.0%
! GGTGTCTGGGGTTCGAACTC+CGG + chr3_3:33183859-33183878 None:intergenic 60.0%
CTAGCAGGAGGTCGCGGTCA+CGG - chr3_3:33183256-33183275 Msa0449480:CDS 65.0%
CTAGCAGGAGGTCGCGGTCA+CGG - chr3_3:33183256-33183275 Msa0449480:CDS 65.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 33183252 33184367 33183252 ID=Msa0449480;Name=Msa0449480
chr3_3 mRNA 33183252 33184367 33183252 ID=Msa0449480-mRNA-1;Parent=Msa0449480;Name=Msa0449480-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0|0|0|1|1|1|2|0|92
chr3_3 exon 33184239 33184367 33184239 ID=Msa0449480-mRNA-1:exon:5945;Parent=Msa0449480-mRNA-1
chr3_3 exon 33183252 33183401 33183252 ID=Msa0449480-mRNA-1:exon:5944;Parent=Msa0449480-mRNA-1
chr3_3 CDS 33184239 33184367 33184239 ID=Msa0449480-mRNA-1:cds;Parent=Msa0449480-mRNA-1
chr3_3 CDS 33183252 33183401 33183252 ID=Msa0449480-mRNA-1:cds;Parent=Msa0449480-mRNA-1
Gene Sequence

>Msa0449480

ATGTCTAGCAGGAGGTCGCGGTCACGGCAAACAAGTAGCTCGAGGAATATCACCGACGATCAGATCCATGATCTTGTCTCCAAGCTGCAGCAACTTCTTCCTGAGATTCGCAATAGGAGCTCTGACAAGGTTTCAGCTTCGAGGGTGTTGCAGGAGACTTGCAACTATATTAGAAACTTGAACAGAGAAGTTGACGACCTAAGTGAGCGTTTGTCTGAGCTATTGGCTACGACAGACACAGCACAAGCAGCAATAATAAGAAATTTACTTATGCAATAG

Protein sequence

>Msa0449480

MSSRRSRSRQTSSSRNITDDQIHDLVSKLQQLLPEIRNRSSDKVSASRVLQETCNYIRNLNREVDDLSERLSELLATTDTAQAAIIRNLLMQ*