AlfalfaGEDB | Msa0000750

M. sativa cultivar zhongmu-4 / Msa0000750

KEGG: K09260
GO: GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0010154 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0042995 GO:0043076 GO:0043078 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0080090 GO:0090406 GO:0097159 GO:0120025 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00319.21

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0000750	sp\|Q7XJK8\|PHE2_ARATH	28.807	243	145	6	6	232	2	232	1.66e-19	92.0

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
Msa0000750	TF	MADS-M-type

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000750	Msa1380290	0.818650	1.754724e-52	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0000750	MtrunA17_Chr4g0008701	77.281	559	62	5	1	534	1	519	0.0	773
Msa0000750	MtrunA17_Chr7g0267601	76.029	559	53	7	1	534	1	503	0.0	759
Msa0000750	MtrunA17_Chr3g0128721	45.408	588	249	12	1	534	1	570	4.08e-139	414
Msa0000750	MtrunA17_Chr8g0361741	50.362	276	128	2	13	283	13	284	5.15e-66	216
Msa0000750	MtrunA17_Chr3g0107381	32.121	165	108	2	1	164	1	162	1.45e-23	99.0
Msa0000750	MtrunA17_Chr5g0432691	33.333	156	102	2	7	161	4	158	1.15e-22	94.7
Msa0000750	MtrunA17_Chr8g0351751	37.405	131	80	1	4	132	1	131	2.45e-21	93.6
Msa0000750	MtrunA17_Chr4g0014321	35.246	122	79	0	6	127	3	124	4.19e-21	92.4
Msa0000750	MtrunA17_Chr4g0013041	30.256	195	124	4	7	196	4	191	6.15e-21	91.3
Msa0000750	MtrunA17_Chr3g0107331	33.333	150	99	1	6	154	2	151	7.07e-20	86.7

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0000750	AT1G65300.1	28.807	243	145	6	6	232	2	232	1.69e-20	92.0
Msa0000750	AT2G40210.1	28.144	167	117	2	4	170	1	164	8.69e-19	88.6
Msa0000750	AT3G05860.3	31.818	132	86	2	1	131	1	129	8.41e-17	80.9
Msa0000750	AT3G05860.1	31.818	132	86	2	1	131	1	129	9.37e-17	80.9
Msa0000750	AT5G26650.1	31.579	114	78	0	7	120	2	115	2.47e-16	81.3
Msa0000750	AT1G31630.1	33.043	115	76	1	6	120	2	115	2.61e-16	80.9
Msa0000750	AT3G05860.2	31.818	132	86	2	1	131	1	129	3.11e-16	78.2
Msa0000750	AT5G27960.1	29.630	135	87	1	7	133	2	136	7.11e-16	79.3
Msa0000750	AT5G06500.1	31.293	147	94	3	6	149	3	145	2.40e-15	76.3
Msa0000750	AT1G31640.1	31.304	115	78	1	6	120	2	115	2.68e-15	79.0

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Find 118 sgRNAs with CRISPR-Local

Find 290 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGATATTGGTGCCAACGG+TGG	0.617732	1_1:+2024013	None:intergenic
AAAGTTGATCAAATGAGCAG+TGG	0.618596	1_1:-2023902	Msa0000750:CDS
AACAACCTTGGAAGTAGCAT+GGG	0.513933	1_1:-2023872	Msa0000750:CDS
AACAATATGGGTAGCAACAT+TGG	0.431457	1_1:-2023656	Msa0000750:CDS
AACACACCTACACCACCACT+AGG	0.650603	1_1:+2023614	None:intergenic
AACGGTGATGGGGTGCATAA+TGG	0.258877	1_1:-2023980	Msa0000750:CDS
AACTTTGGTGGCAATATTAA+TGG	0.198018	1_1:-2023023	Msa0000750:CDS
AACTTTGTTGACAATAATTA+CGG	0.325383	1_1:-2022975	Msa0000750:CDS
AATATGGCCATCTCCTGCAG+TGG	0.655817	1_1:-2024347	Msa0000750:CDS
ACAACACAGGTAGCGGCATG+TGG	0.592125	1_1:-2023427	Msa0000750:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGAGAATAAAACTCGAAGAA+AGG	-	chr1_1:2022982-2023001	Msa0000750:CDS	30.0%
TGCTATAAGAGAAGATTGAA+AGG	-	chr1_1:2023007-2023026	Msa0000750:CDS	30.0%
GCTATAAGAGAAGATTGAAA+GGG	-	chr1_1:2023008-2023027	Msa0000750:CDS	30.0%
GTGATACAAAACCATCAATA+TGG	-	chr1_1:2023101-2023120	Msa0000750:CDS	30.0%
TTGACTCAAACTTGGTTATT+AGG	+	chr1_1:2023150-2023169	None:intergenic	30.0%
TATCCTCCAAAAAAGTCAAT+TGG	+	chr1_1:2023207-2023226	None:intergenic	30.0%
ATGCATCAAATTGAAAACGA+AGG	-	chr1_1:2023298-2023317	Msa0000750:CDS	30.0%
ATGTCTAGGCATTTAGAAAA+GGG	-	chr1_1:2023403-2023422	Msa0000750:CDS	30.0%
TTAATAAAATGCCCCAGATT+TGG	+	chr1_1:2023790-2023809	None:intergenic	30.0%
TTTGAAGGTCAAGTAAACTT+TGG	-	chr1_1:2024003-2024022	Msa0000750:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_1	gene	2022941	2024545	2022941	ID=Msa0000750;Name=Msa0000750
chr1_1	mRNA	2022941	2024545	2022941	ID=Msa0000750-mRNA-1;Parent=Msa0000750;Name=Msa0000750-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|534
chr1_1	exon	2022941	2024545	2022941	ID=Msa0000750-mRNA-1:exon:401;Parent=Msa0000750-mRNA-1
chr1_1	CDS	2022941	2024545	2022941	ID=Msa0000750-mRNA-1:cds;Parent=Msa0000750-mRNA-1

Gene Sequence

>Msa0000750

ATGACTAGAATCAGAAAAAAGAAGATCACATCCAAATTGATTGAGAATAAAACTCGAAGAAAGGCGTGCTATAAGAGAAGATTGAAAGGGTTGTTCACCAAAATGGAGCATGTGACAATCTTATGTGGTATTGAGGCATGTGCGGTTGTGTTTGGGCCAGGTGATACAAAACCATCAATATGGCCATCTCCTGCAGTGGCTGAAGACCTAATAACCAAGTTTGAGTCAATGTCATTGGATGTGCAATCGAAAAATAAGACTGACCAATTGACTTTTTTGGAGGATAAGGGCAAAAAACTTGAAGCAAGCCTTAGAAAGATCAACAAAGAAAATGAAGAGACTTTAATGGGAGCTTATATGCATCAAATTGAAAACGAAGGTAAGTCTTTAGATGATTTTGCTCATGATGTTCTGAATCGTTTGATTGCTTTTACTCTTGAAAAAATTAAGATCACTAGAAAGATGTCTAGGCATTTAGAAAAGGGGATCTTGCCTCTTAATAATCCTCCTCCACCGTTGGCACCAATATCTTTTACTTTACACAACGGTGATGGGGTGCATAATGGGGTTAACATGTATGGACATTTTTTGAACCAACAACCCTTGTCAGATTTGGGCGAGAAAGTTGATCAAATGAGCAGTGGTTTCAATAACAACCTTGGAAGTAGCATGGGGATACCTCCACTTGCAGATCTTAGTGGTGGTGGTTGTGATAACTTGGCTAATCAAGCTAATTTTGGTGGTTTTGATAATAGAATGGGGATACCATCACCTGATTCTAGTGGTGGTTTTGATACAGCGTTTTTTCAAGACAATTTGGAAAATTTTGATAGTATCATGGAGATACCAAATCTGGGGCATTTTATTAACAATATGGGTAGCAACATTGGGATACAATCACATGAAAATCCTAGTGGTGGTGTAGGTGTGTTGCCTCCTCAAGGATATTTTGGAGGTCAAAGCAACTTTCAAGGTTTTGATGACATTACAGGTAGCAACACAGGGATACTACCACATGCAAATCTGAATGGGAATGTTGATTTGTTGCCTCCTCAAGAAAATTTTGAAGGTCAAGTAAACTTTGGATGTTTTGATGACAACACAGGTAGCGGCATGTGGATACCACATTCAAATTCGAGTGGTGGTGATACATTGCTTCCTCAAGAAAATTTTGGAGGCCAATTTAACTTTGAAGATTTTGATATCAACACAGGTAGCAACATGTTGATACCACCACATACAAATCTTGGTGTTGGTGATGTTGATGTGTTGTCTCATCAAGGAAATTTTGGAGTTGATAACAACACAGGTAGCAACATGTTGATACCACCTCATACAAATCCTGGTGTTGGTGATGTTGATGTGTTGTCTCATCAAGGAAATTTTGAAGTTCAAGACAACTTTAGAGGTTTTGATGATAATATATGTAGCAACATGTGGAAACCTCCACATGAAAATCCAAGCAATGGTGGTGTTGATATGATGACTTTTCATCAAAACAACTTTGGTGGCAATATTAATGGAGATGGGATGTGGAGTTTCAATGCAAACTTTGTTGACAATAATTACGGGAGCAACTTCAACCCTGGATTTCCTTCTGAATAA

Protein sequence

>Msa0000750

MTRIRKKKITSKLIENKTRRKACYKRRLKGLFTKMEHVTILCGIEACAVVFGPGDTKPSIWPSPAVAEDLITKFESMSLDVQSKNKTDQLTFLEDKGKKLEASLRKINKENEETLMGAYMHQIENEGKSLDDFAHDVLNRLIAFTLEKIKITRKMSRHLEKGILPLNNPPPPLAPISFTLHNGDGVHNGVNMYGHFLNQQPLSDLGEKVDQMSSGFNNNLGSSMGIPPLADLSGGGCDNLANQANFGGFDNRMGIPSPDSSGGFDTAFFQDNLENFDSIMEIPNLGHFINNMGSNIGIQSHENPSGGVGVLPPQGYFGGQSNFQGFDDITGSNTGILPHANLNGNVDLLPPQENFEGQVNFGCFDDNTGSGMWIPHSNSSGGDTLLPQENFGGQFNFEDFDINTGSNMLIPPHTNLGVGDVDVLSHQGNFGVDNNTGSNMLIPPHTNPGVGDVDVLSHQGNFEVQDNFRGFDDNICSNMWKPPHENPSNGGVDMMTFHQNNFGGNINGDGMWSFNANFVDNNYGSNFNPGFPSE*