AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0001560

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0001560 MtrunA17_Chr3g0145951 99.119 227 2 0 1 227 1 227 1.98e-158 437
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0001560 AT1G16210.1 73.009 226 58 1 1 223 1 226 1.16e-106 307

Find 97 sgRNAs with CRISPR-Local

Find 224 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATCGAGTGTATTTAAAATT+TGG 0.164169 1_1:-3233831 None:intergenic
CGCCTCTTCCTCCCTCTTCT+TGG 0.214378 1_1:-3232848 None:intergenic
AAGACAACTTCAAAGATTAT+TGG 0.247121 1_1:-3233780 None:intergenic
CGTTTCCGCGGCGCTCTTGC+GGG 0.269884 1_1:-3232749 None:intergenic
CGCCGCGGAAACGGAGAAAA+AGG 0.310222 1_1:+3232759 Msa0001560:CDS
AGGACATGATTTGGAAGCTT+TGG 0.331210 1_1:+3233403 Msa0001560:CDS
GCATCTTTAATTCACCTAGT+AGG 0.334052 1_1:-3232684 None:intergenic
ACCCTGTTTGCCTTCTTGTC+AGG 0.352573 1_1:-3232961 None:intergenic
GGTTTCTCTTGCTTCAACTT+AGG 0.359376 1_1:-3233357 None:intergenic
CACAGTACAAGGACATGATT+TGG 0.361884 1_1:+3233394 Msa0001560:CDS
CGCCTCTCTGGATGCCTGTC+GGG 0.367827 1_1:-3233210 None:intergenic
CGCCAAGAAGAGGGAGGAAG+AGG 0.386559 1_1:+3232846 Msa0001560:CDS
CCGCAAGAGCGCCGCGGAAA+CGG 0.388342 1_1:+3232750 Msa0001560:CDS
GAAGCCTTGAGGCGCCTCTC+TGG 0.388885 1_1:-3233222 None:intergenic
GAGTGAGTGACTTGATTAAG+AGG 0.390157 1_1:-3233444 None:intergenic
CCTAGTAGGGTTTCGCTCGC+TGG 0.400470 1_1:-3232670 None:intergenic
GCCTCCGTCACCTTCGGTAC+AGG 0.404250 1_1:-3232991 None:intergenic
GGTGCGTCGATTGGCGGAGC+TGG 0.407431 1_1:+3232906 Msa0001560:CDS
CAAAGATTATTGGTTGTAGT+AGG 0.408363 1_1:-3233770 None:intergenic
CCGTTTCCGCGGCGCTCTTG+CGG 0.414275 1_1:-3232750 None:intergenic
CATCTTTAATTCACCTAGTA+GGG 0.414363 1_1:-3232683 None:intergenic
AGCCTGACAAGAAGGCAAAC+AGG 0.415814 1_1:+3232959 Msa0001560:CDS
AAAGATGGGTGTGAATTCTA+AGG 0.416000 1_1:+3232711 Msa0001560:CDS
GGAGGAAGAGGCGGATAAGA+AGG 0.418038 1_1:+3232858 Msa0001560:CDS
CTTGCGGGCCCTCGCCGCCT+CGG 0.418040 1_1:-3232734 None:intergenic
GGATAAGAAGGCGGAGGCGG+CGG 0.424210 1_1:+3232870 Msa0001560:CDS
GAGAGAAGGAGGAGCAGTAC+TGG 0.439791 1_1:+3232794 Msa0001560:CDS
ACTTGATTAAGAGGATTGTC+AGG 0.442090 1_1:-3233435 None:intergenic
GCAAGCTATGGCACAGAAGA+AGG 0.443447 1_1:+3233041 Msa0001560:CDS
GGCGGATAAGAAGGCGGAGG+CGG 0.454208 1_1:+3232867 Msa0001560:CDS
GAAGAGGACTGCTGGGGAGG+AGG 0.460637 1_1:+3233077 Msa0001560:CDS
TTCTAAGGCCGAGGCGGCGA+GGG 0.465736 1_1:+3232726 Msa0001560:CDS
GAAGCGAAGAAGAGGACTGC+TGG 0.465817 1_1:+3233069 Msa0001560:CDS
GCGCCTCAAGGCTTCCTTCA+AGG 0.472754 1_1:+3233230 Msa0001560:CDS
AGCGGAGGTGCGTCGATTGG+CGG 0.475781 1_1:+3232900 Msa0001560:CDS
AGCTCCGCCTCCGTCACCTT+CGG 0.476514 1_1:-3232997 None:intergenic
AAAACAGATACATACCTTGA+AGG 0.481347 1_1:-3233244 None:intergenic
GCGCCTCTCTGGATGCCTGT+CGG 0.482921 1_1:-3233211 None:intergenic
TACTGTGTGTATGTAAGCCC+TGG 0.485764 1_1:-3233378 None:intergenic
GGAGGCGGCGGCACGACGAG+CGG 0.491424 1_1:+3232882 Msa0001560:CDS
AAGCGAAGAAGAGGACTGCT+GGG 0.493135 1_1:+3233070 Msa0001560:CDS
GATGGCTTCGTCAAGAGTGC+TGG 0.493876 1_1:-3233157 None:intergenic
CGGAGGCGGAGCTGAGGAAG+CGG 0.494922 1_1:+3233007 Msa0001560:CDS
CCAGCGAGCGAAACCCTACT+AGG 0.505208 1_1:+3232670 Msa0001560:five_prime_UTR
GCTGAGGAAGCGGCGAGAGG+AGG 0.505549 1_1:+3233017 Msa0001560:CDS
TCCCTCTTCTTGGCGGCGCG+TGG 0.507668 1_1:-3232838 None:intergenic
CTCTTCCTCCCTCTTCTTGG+CGG 0.507806 1_1:-3232845 None:intergenic
AGATTATTGGTTGTAGTAGG+AGG 0.510390 1_1:-3233767 None:intergenic
GCCACGCGCCGCCAAGAAGA+GGG 0.513629 1_1:+3232837 Msa0001560:CDS
AGCCACGCGCCGCCAAGAAG+AGG 0.516989 1_1:+3232836 Msa0001560:CDS
GCGAGAGGAGGAGCAAGCTA+TGG 0.521774 1_1:+3233029 Msa0001560:CDS
ACGAGCGGAGGTGCGTCGAT+TGG 0.522062 1_1:+3232897 Msa0001560:CDS
GGCGGCGGCACGACGAGCGG+AGG 0.533711 1_1:+3232885 Msa0001560:CDS
ACTTAATCATGAATTTGAGA+TGG 0.534693 1_1:-3233618 None:intergenic
CCTCCGTCACCTTCGGTACA+GGG 0.541244 1_1:-3232990 None:intergenic
GGAGCTGAGGAAGCGGCGAG+AGG 0.552884 1_1:+3233014 Msa0001560:CDS
GCCTGACAAGAAGGCAAACA+GGG 0.555319 1_1:+3232960 Msa0001560:CDS
GCTTACATACACACAGTACA+AGG 0.555636 1_1:+3233383 Msa0001560:CDS
CAAGAAGAGGGAGGAAGAGG+CGG 0.555830 1_1:+3232849 Msa0001560:CDS
GGGTGTGAATTCTAAGGCCG+AGG 0.555835 1_1:+3232717 Msa0001560:CDS
GAATCAATAAAGTCATGGCT+TGG 0.558339 1_1:+3233573 Msa0001560:three_prime_UTR
GAAGAAGAGGACTGCTGGGG+AGG 0.559520 1_1:+3233074 Msa0001560:CDS
AGGTGACGGAGGCGGAGCTG+AGG 0.562961 1_1:+3233001 Msa0001560:CDS
AGAGGCGGATAAGAAGGCGG+AGG 0.563473 1_1:+3232864 Msa0001560:CDS
TGTACCGAAGGTGACGGAGG+CGG 0.565468 1_1:+3232993 Msa0001560:CDS
AGGCGGAGGAAGCGAAGAAG+AGG 0.566562 1_1:+3233061 Msa0001560:CDS
CTCTCTGGATGCCTGTCGGG+AGG 0.568049 1_1:-3233207 None:intergenic
GCGTCGATTGGCGGAGCTGG+AGG 0.569855 1_1:+3232909 Msa0001560:CDS
GCATCCAGAGAGGCGCCTCA+AGG 0.574178 1_1:+3233218 Msa0001560:CDS
ATCGATACTCATCTGAGCGA+TGG 0.576376 1_1:-3233175 None:intergenic
AGCAGCAACTGCTGAAGCAG+AGG 0.579802 1_1:+3232630 Msa0001560:five_prime_UTR
ATTCTAAGGCCGAGGCGGCG+AGG 0.588501 1_1:+3232725 Msa0001560:CDS
TCGGGAGACTCGAGAGAAGG+AGG 0.591271 1_1:+3232783 Msa0001560:CDS
TCAATAAAGTCATGGCTTGG+CGG 0.593024 1_1:+3233576 Msa0001560:three_prime_UTR
ATAACAACTTACCTCCCGAC+AGG 0.598312 1_1:+3233196 Msa0001560:CDS
ACGCGCCGCCAAGAAGAGGG+AGG 0.598349 1_1:+3232840 Msa0001560:CDS
GAGGGCCCGCAAGAGCGCCG+CGG 0.599591 1_1:+3232744 Msa0001560:CDS
GGAGCTGGAGGAGAAAGAGC+TGG 0.599937 1_1:+3232921 Msa0001560:CDS
AAGTTGAAGCAAGAGAAACC+AGG 0.604189 1_1:+3233360 Msa0001560:CDS
ATGATGAATCAATAAAGTCA+TGG 0.605965 1_1:+3233568 Msa0001560:three_prime_UTR
GGATCGGGAGACTCGAGAGA+AGG 0.606508 1_1:+3232780 Msa0001560:CDS
CCCTGTACCGAAGGTGACGG+AGG 0.608676 1_1:+3232990 Msa0001560:CDS
TATGGCACAGAAGAAGGCGG+AGG 0.610578 1_1:+3233047 Msa0001560:CDS
GGAGGAGGAGTACGAGAGAG+TGG 0.614209 1_1:+3233092 Msa0001560:CDS
GGTGTCTATCCCTGTACCGA+AGG 0.615935 1_1:+3232981 Msa0001560:CDS
TTATTGATTCATCATTCACT+CGG 0.617967 1_1:-3233560 None:intergenic
GGAAGAGGCGGATAAGAAGG+CGG 0.620199 1_1:+3232861 Msa0001560:CDS
TATCCCTGTACCGAAGGTGA+CGG 0.630098 1_1:+3232987 Msa0001560:CDS
CTCCCGACAGGCATCCAGAG+AGG 0.637354 1_1:+3233208 Msa0001560:CDS
GGAGGAGCAGTACTGGCGAG+AGG 0.649799 1_1:+3232801 Msa0001560:CDS
TCATATACTACTGCTTACAG+AGG 0.659085 1_1:-3233675 None:intergenic
TGTGAATTCTAAGGCCGAGG+CGG 0.660597 1_1:+3232720 Msa0001560:CDS
AGTTGAAGCAAGAGAAACCA+GGG 0.661223 1_1:+3233361 Msa0001560:CDS
CATATACTACTGCTTACAGA+GGG 0.665172 1_1:-3233674 None:intergenic
AGCGAAGAAGAGGACTGCTG+GGG 0.681467 1_1:+3233071 Msa0001560:CDS
ATACCTTGAAGGAAGCCTTG+AGG 0.684866 1_1:-3233233 None:intergenic
AGCTATGGCACAGAAGAAGG+CGG 0.695332 1_1:+3233044 Msa0001560:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGATAACAAAAATAAAAGAT+AGG - chr1_1:3233506-3233525 None:intergenic 15.0%
!! AGATAACAAAAATAAAAGAT+AGG - chr1_1:3233506-3233525 None:intergenic 15.0%
!!! TATCGAGTGTATTTAAAATT+TGG - chr1_1:3233834-3233853 None:intergenic 20.0%
!!! TATCGAGTGTATTTAAAATT+TGG - chr1_1:3233834-3233853 None:intergenic 20.0%
! ATGAATGAATGAATGAATGA+AGG - chr1_1:3233309-3233328 None:intergenic 25.0%
! AATAAAAGATAGGCTAAAAG+TGG - chr1_1:3233496-3233515 None:intergenic 25.0%
!!! TTATTGATTCATCATTCACT+CGG - chr1_1:3233563-3233582 None:intergenic 25.0%
! ATGATGAATCAATAAAGTCA+TGG + chr1_1:3233568-3233587 Msa0001560:three_prime_UTR 25.0%
! ACTTAATCATGAATTTGAGA+TGG - chr1_1:3233621-3233640 None:intergenic 25.0%
!!! ACAAACATTGTTTGTTTTCA+TGG + chr1_1:3233727-3233746 Msa0001560:three_prime_UTR 25.0%
! AAGACAACTTCAAAGATTAT+TGG - chr1_1:3233783-3233802 None:intergenic 25.0%
! ATGAATGAATGAATGAATGA+AGG - chr1_1:3233309-3233328 None:intergenic 25.0%
! AATAAAAGATAGGCTAAAAG+TGG - chr1_1:3233496-3233515 None:intergenic 25.0%
!!! TTATTGATTCATCATTCACT+CGG - chr1_1:3233563-3233582 None:intergenic 25.0%
! ATGATGAATCAATAAAGTCA+TGG + chr1_1:3233568-3233587 Msa0001560:three_prime_UTR 25.0%
! ACTTAATCATGAATTTGAGA+TGG - chr1_1:3233621-3233640 None:intergenic 25.0%
!!! ACAAACATTGTTTGTTTTCA+TGG + chr1_1:3233727-3233746 Msa0001560:three_prime_UTR 25.0%
! AAGACAACTTCAAAGATTAT+TGG - chr1_1:3233783-3233802 None:intergenic 25.0%
CATCTTTAATTCACCTAGTA+GGG - chr1_1:3232686-3232705 None:intergenic 30.0%
ATTAAAGATGCCCAAAAAGA+TGG + chr1_1:3232696-3232715 Msa0001560:exon 30.0%
TTAAAGATGCCCAAAAAGAT+GGG + chr1_1:3232697-3232716 Msa0001560:exon 30.0%
AAAACAGATACATACCTTGA+AGG - chr1_1:3233247-3233266 None:intergenic 30.0%
CATTCATTCATTCATTCATC+AGG + chr1_1:3233314-3233333 Msa0001560:intron 30.0%
!! CTAAAAGTGGATTATAAAGG+AGG - chr1_1:3233483-3233502 None:intergenic 30.0%
!! AGGCTAAAAGTGGATTATAA+AGG - chr1_1:3233486-3233505 None:intergenic 30.0%
! CAAAGATTATTGGTTGTAGT+AGG - chr1_1:3233773-3233792 None:intergenic 30.0%
CATCTTTAATTCACCTAGTA+GGG - chr1_1:3232686-3232705 None:intergenic 30.0%
ATTAAAGATGCCCAAAAAGA+TGG + chr1_1:3232696-3232715 Msa0001560:exon 30.0%
TTAAAGATGCCCAAAAAGAT+GGG + chr1_1:3232697-3232716 Msa0001560:exon 30.0%
AAAACAGATACATACCTTGA+AGG - chr1_1:3233247-3233266 None:intergenic 30.0%
CATTCATTCATTCATTCATC+AGG + chr1_1:3233314-3233333 Msa0001560:intron 30.0%
!! CTAAAAGTGGATTATAAAGG+AGG - chr1_1:3233483-3233502 None:intergenic 30.0%
!! AGGCTAAAAGTGGATTATAA+AGG - chr1_1:3233486-3233505 None:intergenic 30.0%
! CAAAGATTATTGGTTGTAGT+AGG - chr1_1:3233773-3233792 None:intergenic 30.0%
GCATCTTTAATTCACCTAGT+AGG - chr1_1:3232687-3232706 None:intergenic 35.0%
! GAATTCACACCCATCTTTTT+GGG - chr1_1:3232709-3232728 None:intergenic 35.0%
! AGAATTCACACCCATCTTTT+TGG - chr1_1:3232710-3232729 None:intergenic 35.0%
AAAGATGGGTGTGAATTCTA+AGG + chr1_1:3232711-3232730 Msa0001560:CDS 35.0%
GATTAAAAAGCCTGACAAGA+AGG + chr1_1:3232951-3232970 Msa0001560:CDS 35.0%
ACTTGATTAAGAGGATTGTC+AGG - chr1_1:3233438-3233457 None:intergenic 35.0%
GAATCAATAAAGTCATGGCT+TGG + chr1_1:3233573-3233592 Msa0001560:three_prime_UTR 35.0%
CATATACTACTGCTTACAGA+GGG - chr1_1:3233677-3233696 None:intergenic 35.0%
TCATATACTACTGCTTACAG+AGG - chr1_1:3233678-3233697 None:intergenic 35.0%
! AGATTATTGGTTGTAGTAGG+AGG - chr1_1:3233770-3233789 None:intergenic 35.0%
GCATCTTTAATTCACCTAGT+AGG - chr1_1:3232687-3232706 None:intergenic 35.0%
! GAATTCACACCCATCTTTTT+GGG - chr1_1:3232709-3232728 None:intergenic 35.0%
! AGAATTCACACCCATCTTTT+TGG - chr1_1:3232710-3232729 None:intergenic 35.0%
AAAGATGGGTGTGAATTCTA+AGG + chr1_1:3232711-3232730 Msa0001560:CDS 35.0%
GATTAAAAAGCCTGACAAGA+AGG + chr1_1:3232951-3232970 Msa0001560:CDS 35.0%
ACTTGATTAAGAGGATTGTC+AGG - chr1_1:3233438-3233457 None:intergenic 35.0%
GAATCAATAAAGTCATGGCT+TGG + chr1_1:3233573-3233592 Msa0001560:three_prime_UTR 35.0%
CATATACTACTGCTTACAGA+GGG - chr1_1:3233677-3233696 None:intergenic 35.0%
TCATATACTACTGCTTACAG+AGG - chr1_1:3233678-3233697 None:intergenic 35.0%
! AGATTATTGGTTGTAGTAGG+AGG - chr1_1:3233770-3233789 None:intergenic 35.0%
GGTTTCTCTTGCTTCAACTT+AGG - chr1_1:3233360-3233379 None:intergenic 40.0%
AAGTTGAAGCAAGAGAAACC+AGG + chr1_1:3233360-3233379 Msa0001560:CDS 40.0%
AGTTGAAGCAAGAGAAACCA+GGG + chr1_1:3233361-3233380 Msa0001560:CDS 40.0%
GCTTACATACACACAGTACA+AGG + chr1_1:3233383-3233402 Msa0001560:CDS 40.0%
CACAGTACAAGGACATGATT+TGG + chr1_1:3233394-3233413 Msa0001560:CDS 40.0%
! AGGACATGATTTGGAAGCTT+TGG + chr1_1:3233403-3233422 Msa0001560:CDS 40.0%
GAGTGAGTGACTTGATTAAG+AGG - chr1_1:3233447-3233466 None:intergenic 40.0%
! TCAATAAAGTCATGGCTTGG+CGG + chr1_1:3233576-3233595 Msa0001560:three_prime_UTR 40.0%
GGTTTCTCTTGCTTCAACTT+AGG - chr1_1:3233360-3233379 None:intergenic 40.0%
AAGTTGAAGCAAGAGAAACC+AGG + chr1_1:3233360-3233379 Msa0001560:CDS 40.0%
AGTTGAAGCAAGAGAAACCA+GGG + chr1_1:3233361-3233380 Msa0001560:CDS 40.0%
GCTTACATACACACAGTACA+AGG + chr1_1:3233383-3233402 Msa0001560:CDS 40.0%
CACAGTACAAGGACATGATT+TGG + chr1_1:3233394-3233413 Msa0001560:CDS 40.0%
! AGGACATGATTTGGAAGCTT+TGG + chr1_1:3233403-3233422 Msa0001560:CDS 40.0%
GAGTGAGTGACTTGATTAAG+AGG - chr1_1:3233447-3233466 None:intergenic 40.0%
! TCAATAAAGTCATGGCTTGG+CGG + chr1_1:3233576-3233595 Msa0001560:three_prime_UTR 40.0%
! ATCCTTTTTCTCCGTTTCCG+CGG - chr1_1:3232764-3232783 None:intergenic 45.0%
CGGAAACGGAGAAAAAGGAT+CGG + chr1_1:3232764-3232783 Msa0001560:CDS 45.0%
GGAAACGGAGAAAAAGGATC+GGG + chr1_1:3232765-3232784 Msa0001560:CDS 45.0%
ATCGATACTCATCTGAGCGA+TGG - chr1_1:3233178-3233197 None:intergenic 45.0%
ATAACAACTTACCTCCCGAC+AGG + chr1_1:3233196-3233215 Msa0001560:CDS 45.0%
ATACCTTGAAGGAAGCCTTG+AGG - chr1_1:3233236-3233255 None:intergenic 45.0%
TACTGTGTGTATGTAAGCCC+TGG - chr1_1:3233381-3233400 None:intergenic 45.0%
! ATCCTTTTTCTCCGTTTCCG+CGG - chr1_1:3232764-3232783 None:intergenic 45.0%
CGGAAACGGAGAAAAAGGAT+CGG + chr1_1:3232764-3232783 Msa0001560:CDS 45.0%
GGAAACGGAGAAAAAGGATC+GGG + chr1_1:3232765-3232784 Msa0001560:CDS 45.0%
ATCGATACTCATCTGAGCGA+TGG - chr1_1:3233178-3233197 None:intergenic 45.0%
ATAACAACTTACCTCCCGAC+AGG + chr1_1:3233196-3233215 Msa0001560:CDS 45.0%
ATACCTTGAAGGAAGCCTTG+AGG - chr1_1:3233236-3233255 None:intergenic 45.0%
TACTGTGTGTATGTAAGCCC+TGG - chr1_1:3233381-3233400 None:intergenic 45.0%
!! TGTGAATTCTAAGGCCGAGG+CGG + chr1_1:3232720-3232739 Msa0001560:CDS 50.0%
AGCCTGACAAGAAGGCAAAC+AGG + chr1_1:3232959-3232978 Msa0001560:CDS 50.0%
GCCTGACAAGAAGGCAAACA+GGG + chr1_1:3232960-3232979 Msa0001560:CDS 50.0%
ACCCTGTTTGCCTTCTTGTC+AGG - chr1_1:3232964-3232983 None:intergenic 50.0%
TATCCCTGTACCGAAGGTGA+CGG + chr1_1:3232987-3233006 Msa0001560:CDS 50.0%
GCAAGCTATGGCACAGAAGA+AGG + chr1_1:3233041-3233060 Msa0001560:CDS 50.0%
AGCTATGGCACAGAAGAAGG+CGG + chr1_1:3233044-3233063 Msa0001560:CDS 50.0%
AAGCGAAGAAGAGGACTGCT+GGG + chr1_1:3233070-3233089 Msa0001560:CDS 50.0%
!! TGTGAATTCTAAGGCCGAGG+CGG + chr1_1:3232720-3232739 Msa0001560:CDS 50.0%
AGCCTGACAAGAAGGCAAAC+AGG + chr1_1:3232959-3232978 Msa0001560:CDS 50.0%
GCCTGACAAGAAGGCAAACA+GGG + chr1_1:3232960-3232979 Msa0001560:CDS 50.0%
ACCCTGTTTGCCTTCTTGTC+AGG - chr1_1:3232964-3232983 None:intergenic 50.0%
TATCCCTGTACCGAAGGTGA+CGG + chr1_1:3232987-3233006 Msa0001560:CDS 50.0%
GCAAGCTATGGCACAGAAGA+AGG + chr1_1:3233041-3233060 Msa0001560:CDS 50.0%
AGCTATGGCACAGAAGAAGG+CGG + chr1_1:3233044-3233063 Msa0001560:CDS 50.0%
AAGCGAAGAAGAGGACTGCT+GGG + chr1_1:3233070-3233089 Msa0001560:CDS 50.0%
AGCAGCAACTGCTGAAGCAG+AGG + chr1_1:3232630-3232649 Msa0001560:five_prime_UTR 55.0%
!! GGGTGTGAATTCTAAGGCCG+AGG + chr1_1:3232717-3232736 Msa0001560:CDS 55.0%
GAGAGAAGGAGGAGCAGTAC+TGG + chr1_1:3232794-3232813 Msa0001560:CDS 55.0%
CTCTTCCTCCCTCTTCTTGG+CGG - chr1_1:3232848-3232867 None:intergenic 55.0%
CAAGAAGAGGGAGGAAGAGG+CGG + chr1_1:3232849-3232868 Msa0001560:CDS 55.0%
GGAGGAAGAGGCGGATAAGA+AGG + chr1_1:3232858-3232877 Msa0001560:CDS 55.0%
GGAAGAGGCGGATAAGAAGG+CGG + chr1_1:3232861-3232880 Msa0001560:CDS 55.0%
GGTGTCTATCCCTGTACCGA+AGG + chr1_1:3232981-3233000 Msa0001560:CDS 55.0%
TATGGCACAGAAGAAGGCGG+AGG + chr1_1:3233047-3233066 Msa0001560:CDS 55.0%
GAAGCGAAGAAGAGGACTGC+TGG + chr1_1:3233069-3233088 Msa0001560:CDS 55.0%
AGCGAAGAAGAGGACTGCTG+GGG + chr1_1:3233071-3233090 Msa0001560:CDS 55.0%
! GATGGCTTCGTCAAGAGTGC+TGG - chr1_1:3233160-3233179 None:intergenic 55.0%
AGCAGCAACTGCTGAAGCAG+AGG + chr1_1:3232630-3232649 Msa0001560:five_prime_UTR 55.0%
!! GGGTGTGAATTCTAAGGCCG+AGG + chr1_1:3232717-3232736 Msa0001560:CDS 55.0%
GAGAGAAGGAGGAGCAGTAC+TGG + chr1_1:3232794-3232813 Msa0001560:CDS 55.0%
CTCTTCCTCCCTCTTCTTGG+CGG - chr1_1:3232848-3232867 None:intergenic 55.0%
CAAGAAGAGGGAGGAAGAGG+CGG + chr1_1:3232849-3232868 Msa0001560:CDS 55.0%
GGAGGAAGAGGCGGATAAGA+AGG + chr1_1:3232858-3232877 Msa0001560:CDS 55.0%
GGAAGAGGCGGATAAGAAGG+CGG + chr1_1:3232861-3232880 Msa0001560:CDS 55.0%
GGTGTCTATCCCTGTACCGA+AGG + chr1_1:3232981-3233000 Msa0001560:CDS 55.0%
TATGGCACAGAAGAAGGCGG+AGG + chr1_1:3233047-3233066 Msa0001560:CDS 55.0%
GAAGCGAAGAAGAGGACTGC+TGG + chr1_1:3233069-3233088 Msa0001560:CDS 55.0%
AGCGAAGAAGAGGACTGCTG+GGG + chr1_1:3233071-3233090 Msa0001560:CDS 55.0%
! GATGGCTTCGTCAAGAGTGC+TGG - chr1_1:3233160-3233179 None:intergenic 55.0%
CCTAGTAGGGTTTCGCTCGC+TGG - chr1_1:3232673-3232692 None:intergenic 60.0%
CCAGCGAGCGAAACCCTACT+AGG + chr1_1:3232670-3232689 Msa0001560:five_prime_UTR 60.0%
CGCCGCGGAAACGGAGAAAA+AGG + chr1_1:3232759-3232778 Msa0001560:CDS 60.0%
GGATCGGGAGACTCGAGAGA+AGG + chr1_1:3232780-3232799 Msa0001560:CDS 60.0%
TCGGGAGACTCGAGAGAAGG+AGG + chr1_1:3232783-3232802 Msa0001560:CDS 60.0%
CGCCAAGAAGAGGGAGGAAG+AGG + chr1_1:3232846-3232865 Msa0001560:CDS 60.0%
CGCCTCTTCCTCCCTCTTCT+TGG - chr1_1:3232851-3232870 None:intergenic 60.0%
AGAGGCGGATAAGAAGGCGG+AGG + chr1_1:3232864-3232883 Msa0001560:CDS 60.0%
GGAGCTGGAGGAGAAAGAGC+TGG + chr1_1:3232921-3232940 Msa0001560:CDS 60.0%
CCTCCGTCACCTTCGGTACA+GGG - chr1_1:3232993-3233012 None:intergenic 60.0%
!! TGTACCGAAGGTGACGGAGG+CGG + chr1_1:3232993-3233012 Msa0001560:CDS 60.0%
GCGAGAGGAGGAGCAAGCTA+TGG + chr1_1:3233029-3233048 Msa0001560:CDS 60.0%
AGGCGGAGGAAGCGAAGAAG+AGG + chr1_1:3233061-3233080 Msa0001560:CDS 60.0%
GAAGAAGAGGACTGCTGGGG+AGG + chr1_1:3233074-3233093 Msa0001560:CDS 60.0%
! GGAGGAGGAGTACGAGAGAG+TGG + chr1_1:3233092-3233111 Msa0001560:CDS 60.0%
GCGCCTCAAGGCTTCCTTCA+AGG + chr1_1:3233230-3233249 Msa0001560:CDS 60.0%
CCTAGTAGGGTTTCGCTCGC+TGG - chr1_1:3232673-3232692 None:intergenic 60.0%
CCAGCGAGCGAAACCCTACT+AGG + chr1_1:3232670-3232689 Msa0001560:five_prime_UTR 60.0%
CGCCGCGGAAACGGAGAAAA+AGG + chr1_1:3232759-3232778 Msa0001560:CDS 60.0%
GGATCGGGAGACTCGAGAGA+AGG + chr1_1:3232780-3232799 Msa0001560:CDS 60.0%
TCGGGAGACTCGAGAGAAGG+AGG + chr1_1:3232783-3232802 Msa0001560:CDS 60.0%
CGCCAAGAAGAGGGAGGAAG+AGG + chr1_1:3232846-3232865 Msa0001560:CDS 60.0%
CGCCTCTTCCTCCCTCTTCT+TGG - chr1_1:3232851-3232870 None:intergenic 60.0%
AGAGGCGGATAAGAAGGCGG+AGG + chr1_1:3232864-3232883 Msa0001560:CDS 60.0%
GGAGCTGGAGGAGAAAGAGC+TGG + chr1_1:3232921-3232940 Msa0001560:CDS 60.0%
CCTCCGTCACCTTCGGTACA+GGG - chr1_1:3232993-3233012 None:intergenic 60.0%
!! TGTACCGAAGGTGACGGAGG+CGG + chr1_1:3232993-3233012 Msa0001560:CDS 60.0%
GCGAGAGGAGGAGCAAGCTA+TGG + chr1_1:3233029-3233048 Msa0001560:CDS 60.0%
AGGCGGAGGAAGCGAAGAAG+AGG + chr1_1:3233061-3233080 Msa0001560:CDS 60.0%
GAAGAAGAGGACTGCTGGGG+AGG + chr1_1:3233074-3233093 Msa0001560:CDS 60.0%
! GGAGGAGGAGTACGAGAGAG+TGG + chr1_1:3233092-3233111 Msa0001560:CDS 60.0%
GCGCCTCAAGGCTTCCTTCA+AGG + chr1_1:3233230-3233249 Msa0001560:CDS 60.0%
!! ATTCTAAGGCCGAGGCGGCG+AGG + chr1_1:3232725-3232744 Msa0001560:CDS 65.0%
!! TTCTAAGGCCGAGGCGGCGA+GGG + chr1_1:3232726-3232745 Msa0001560:CDS 65.0%
!! GGAGGAGCAGTACTGGCGAG+AGG + chr1_1:3232801-3232820 Msa0001560:CDS 65.0%
GGCGGATAAGAAGGCGGAGG+CGG + chr1_1:3232867-3232886 Msa0001560:CDS 65.0%
GGATAAGAAGGCGGAGGCGG+CGG + chr1_1:3232870-3232889 Msa0001560:CDS 65.0%
! ACGAGCGGAGGTGCGTCGAT+TGG + chr1_1:3232897-3232916 Msa0001560:CDS 65.0%
!! AGCGGAGGTGCGTCGATTGG+CGG + chr1_1:3232900-3232919 Msa0001560:CDS 65.0%
! CCCTGTACCGAAGGTGACGG+AGG + chr1_1:3232990-3233009 Msa0001560:CDS 65.0%
GCCTCCGTCACCTTCGGTAC+AGG - chr1_1:3232994-3233013 None:intergenic 65.0%
AGCTCCGCCTCCGTCACCTT+CGG - chr1_1:3233000-3233019 None:intergenic 65.0%
GAAGAGGACTGCTGGGGAGG+AGG + chr1_1:3233077-3233096 Msa0001560:CDS 65.0%
CTCTCTGGATGCCTGTCGGG+AGG - chr1_1:3233210-3233229 None:intergenic 65.0%
CTCCCGACAGGCATCCAGAG+AGG + chr1_1:3233208-3233227 Msa0001560:CDS 65.0%
CGCCTCTCTGGATGCCTGTC+GGG - chr1_1:3233213-3233232 None:intergenic 65.0%
GCGCCTCTCTGGATGCCTGT+CGG - chr1_1:3233214-3233233 None:intergenic 65.0%
!! GCATCCAGAGAGGCGCCTCA+AGG + chr1_1:3233218-3233237 Msa0001560:CDS 65.0%
! GAAGCCTTGAGGCGCCTCTC+TGG - chr1_1:3233225-3233244 None:intergenic 65.0%
!! ATTCTAAGGCCGAGGCGGCG+AGG + chr1_1:3232725-3232744 Msa0001560:CDS 65.0%
!! TTCTAAGGCCGAGGCGGCGA+GGG + chr1_1:3232726-3232745 Msa0001560:CDS 65.0%
!! GGAGGAGCAGTACTGGCGAG+AGG + chr1_1:3232801-3232820 Msa0001560:CDS 65.0%
GGCGGATAAGAAGGCGGAGG+CGG + chr1_1:3232867-3232886 Msa0001560:CDS 65.0%
GGATAAGAAGGCGGAGGCGG+CGG + chr1_1:3232870-3232889 Msa0001560:CDS 65.0%
! ACGAGCGGAGGTGCGTCGAT+TGG + chr1_1:3232897-3232916 Msa0001560:CDS 65.0%
!! AGCGGAGGTGCGTCGATTGG+CGG + chr1_1:3232900-3232919 Msa0001560:CDS 65.0%
! CCCTGTACCGAAGGTGACGG+AGG + chr1_1:3232990-3233009 Msa0001560:CDS 65.0%
GCCTCCGTCACCTTCGGTAC+AGG - chr1_1:3232994-3233013 None:intergenic 65.0%
AGCTCCGCCTCCGTCACCTT+CGG - chr1_1:3233000-3233019 None:intergenic 65.0%
GAAGAGGACTGCTGGGGAGG+AGG + chr1_1:3233077-3233096 Msa0001560:CDS 65.0%
CTCTCTGGATGCCTGTCGGG+AGG - chr1_1:3233210-3233229 None:intergenic 65.0%
CTCCCGACAGGCATCCAGAG+AGG + chr1_1:3233208-3233227 Msa0001560:CDS 65.0%
CGCCTCTCTGGATGCCTGTC+GGG - chr1_1:3233213-3233232 None:intergenic 65.0%
GCGCCTCTCTGGATGCCTGT+CGG - chr1_1:3233214-3233233 None:intergenic 65.0%
!! GCATCCAGAGAGGCGCCTCA+AGG + chr1_1:3233218-3233237 Msa0001560:CDS 65.0%
! GAAGCCTTGAGGCGCCTCTC+TGG - chr1_1:3233225-3233244 None:intergenic 65.0%
CGTTTCCGCGGCGCTCTTGC+GGG - chr1_1:3232752-3232771 None:intergenic 70.0%
CCGTTTCCGCGGCGCTCTTG+CGG - chr1_1:3232753-3232772 None:intergenic 70.0%
!! CCGCAAGAGCGCCGCGGAAA+CGG + chr1_1:3232750-3232769 Msa0001560:CDS 70.0%
AGCCACGCGCCGCCAAGAAG+AGG + chr1_1:3232836-3232855 Msa0001560:CDS 70.0%
GCCACGCGCCGCCAAGAAGA+GGG + chr1_1:3232837-3232856 Msa0001560:CDS 70.0%
TCCCTCTTCTTGGCGGCGCG+TGG - chr1_1:3232841-3232860 None:intergenic 70.0%
ACGCGCCGCCAAGAAGAGGG+AGG + chr1_1:3232840-3232859 Msa0001560:CDS 70.0%
!! GGTGCGTCGATTGGCGGAGC+TGG + chr1_1:3232906-3232925 Msa0001560:CDS 70.0%
!! GCGTCGATTGGCGGAGCTGG+AGG + chr1_1:3232909-3232928 Msa0001560:CDS 70.0%
!! AGGTGACGGAGGCGGAGCTG+AGG + chr1_1:3233001-3233020 Msa0001560:CDS 70.0%
CGGAGGCGGAGCTGAGGAAG+CGG + chr1_1:3233007-3233026 Msa0001560:CDS 70.0%
GGAGCTGAGGAAGCGGCGAG+AGG + chr1_1:3233014-3233033 Msa0001560:CDS 70.0%
GCTGAGGAAGCGGCGAGAGG+AGG + chr1_1:3233017-3233036 Msa0001560:CDS 70.0%
CGTTTCCGCGGCGCTCTTGC+GGG - chr1_1:3232752-3232771 None:intergenic 70.0%
CCGTTTCCGCGGCGCTCTTG+CGG - chr1_1:3232753-3232772 None:intergenic 70.0%
!! CCGCAAGAGCGCCGCGGAAA+CGG + chr1_1:3232750-3232769 Msa0001560:CDS 70.0%
AGCCACGCGCCGCCAAGAAG+AGG + chr1_1:3232836-3232855 Msa0001560:CDS 70.0%
GCCACGCGCCGCCAAGAAGA+GGG + chr1_1:3232837-3232856 Msa0001560:CDS 70.0%
TCCCTCTTCTTGGCGGCGCG+TGG - chr1_1:3232841-3232860 None:intergenic 70.0%
ACGCGCCGCCAAGAAGAGGG+AGG + chr1_1:3232840-3232859 Msa0001560:CDS 70.0%
!! GGTGCGTCGATTGGCGGAGC+TGG + chr1_1:3232906-3232925 Msa0001560:CDS 70.0%
!! GCGTCGATTGGCGGAGCTGG+AGG + chr1_1:3232909-3232928 Msa0001560:CDS 70.0%
!! AGGTGACGGAGGCGGAGCTG+AGG + chr1_1:3233001-3233020 Msa0001560:CDS 70.0%
CGGAGGCGGAGCTGAGGAAG+CGG + chr1_1:3233007-3233026 Msa0001560:CDS 70.0%
GGAGCTGAGGAAGCGGCGAG+AGG + chr1_1:3233014-3233033 Msa0001560:CDS 70.0%
GCTGAGGAAGCGGCGAGAGG+AGG + chr1_1:3233017-3233036 Msa0001560:CDS 70.0%
! CTTGCGGGCCCTCGCCGCCT+CGG - chr1_1:3232737-3232756 None:intergenic 80.0%
!!! GAGGGCCCGCAAGAGCGCCG+CGG + chr1_1:3232744-3232763 Msa0001560:CDS 80.0%
! GGAGGCGGCGGCACGACGAG+CGG + chr1_1:3232882-3232901 Msa0001560:CDS 80.0%
! CTTGCGGGCCCTCGCCGCCT+CGG - chr1_1:3232737-3232756 None:intergenic 80.0%
!!! GAGGGCCCGCAAGAGCGCCG+CGG + chr1_1:3232744-3232763 Msa0001560:CDS 80.0%
! GGAGGCGGCGGCACGACGAG+CGG + chr1_1:3232882-3232901 Msa0001560:CDS 80.0%
! GGCGGCGGCACGACGAGCGG+AGG + chr1_1:3232885-3232904 Msa0001560:CDS 85.0%
! GGCGGCGGCACGACGAGCGG+AGG + chr1_1:3232885-3232904 Msa0001560:CDS 85.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 3232611 3233878 3232611 ID=Msa0001560;Name=Msa0001560
chr1_1 mRNA 3232611 3233878 3232611 ID=Msa0001560-mRNA-1;Parent=Msa0001560;Name=Msa0001560-mRNA-1;_AED=0.06;_eAED=0.06;_QI=92|1|1|1|1|1|3|311|227
chr1_1 exon 3232611 3233251 3232611 ID=Msa0001560-mRNA-1:exon:818;Parent=Msa0001560-mRNA-1
chr1_1 exon 3233336 3233455 3233336 ID=Msa0001560-mRNA-1:exon:819;Parent=Msa0001560-mRNA-1
chr1_1 exon 3233553 3233878 3233553 ID=Msa0001560-mRNA-1:exon:820;Parent=Msa0001560-mRNA-1
chr1_1 five_prime_UTR 3232611 3232702 3232611 ID=Msa0001560-mRNA-1:five_prime_utr;Parent=Msa0001560-mRNA-1
chr1_1 CDS 3232703 3233251 3232703 ID=Msa0001560-mRNA-1:cds;Parent=Msa0001560-mRNA-1
chr1_1 CDS 3233336 3233455 3233336 ID=Msa0001560-mRNA-1:cds;Parent=Msa0001560-mRNA-1
chr1_1 CDS 3233553 3233567 3233553 ID=Msa0001560-mRNA-1:cds;Parent=Msa0001560-mRNA-1
chr1_1 three_prime_UTR 3233568 3233878 3233568 ID=Msa0001560-mRNA-1:three_prime_utr;Parent=Msa0001560-mRNA-1
Gene Sequence

>Msa0001560

ATGCCCAAAAAGATGGGTGTGAATTCTAAGGCCGAGGCGGCGAGGGCCCGCAAGAGCGCCGCGGAAACGGAGAAAAAGGATCGGGAGACTCGAGAGAAGGAGGAGCAGTACTGGCGAGAGGCAGAGAGTTCAAAGCCACGCGCCGCCAAGAAGAGGGAGGAAGAGGCGGATAAGAAGGCGGAGGCGGCGGCACGACGAGCGGAGGTGCGTCGATTGGCGGAGCTGGAGGAGAAAGAGCTGGAGAAGATGATTAAAAAGCCTGACAAGAAGGCAAACAGGGTGTCTATCCCTGTACCGAAGGTGACGGAGGCGGAGCTGAGGAAGCGGCGAGAGGAGGAGCAAGCTATGGCACAGAAGAAGGCGGAGGAAGCGAAGAAGAGGACTGCTGGGGAGGAGGAGTACGAGAGAGTGGTGCTTGTGTCGAATACGAATCGTGATGATTCAATTATCGAAGCCAGCACTCTTGACGAAGCCATCGCTCAGATGAGTATCGATAACAACTTACCTCCCGACAGGCATCCAGAGAGGCGCCTCAAGGCTTCCTTCAAGGCATTTGAAGAAGCTGAACTACCTAAGTTGAAGCAAGAGAAACCAGGGCTTACATACACACAGTACAAGGACATGATTTGGAAGCTTTGGAAGAAATCTCCTGACAATCCTCTTAATCAAATTGCTGCCGAGTGA
Protein sequence

>Msa0001560

MPKKMGVNSKAEAARARKSAAETEKKDRETREKEEQYWREAESSKPRAAKKREEEADKKAEAAARRAEVRRLAELEEKELEKMIKKPDKKANRVSIPVPKVTEAELRKRREEEQAMAQKKAEEAKKRTAGEEEYERVVLVSNTNRDDSIIEASTLDEAIAQMSIDNNLPPDRHPERRLKASFKAFEEAELPKLKQEKPGLTYTQYKDMIWKLWKKSPDNPLNQIAAE*