Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0004230 | Msa1327640 | 0.806985 | 6.305257e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0004230 | MtrunA17_Chr1g0156781 | 98.214 | 112 | 2 | 0 | 1 | 112 | 1 | 112 | 1.88e-79 | 228 |
| Msa0004230 | MtrunA17_Chr3g0142831 | 53.211 | 109 | 44 | 2 | 1 | 109 | 1 | 102 | 6.44e-32 | 108 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0004230 | AT1G75810.1 | 46.218 | 119 | 56 | 1 | 1 | 111 | 4 | 122 | 1.56e-30 | 105 |
Find 23 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACGTTGGTGGCTTTGATTT+TGG | 0.307158 | 1_1:-6676326 | Msa0004230:CDS |
| TCCACCTTTACAATAAGATA+TGG | 0.350828 | 1_1:+6676219 | None:intergenic |
| ATTGTAAAGGTGGAATTTGT+TGG | 0.381604 | 1_1:-6676210 | Msa0004230:CDS |
| GGCAGTTACATCGTTTGTTA+AGG | 0.431057 | 1_1:-6676407 | Msa0004230:CDS |
| CGGCGTCGCGGATCGCTCGC+CGG | 0.441536 | 1_1:-6676185 | Msa0004230:CDS |
| AATAAGATATGGTATTGTGC+TGG | 0.454277 | 1_1:+6676230 | None:intergenic |
| ACGATTTAGTGGTGTGTTTG+TGG | 0.454337 | 1_1:-6676362 | Msa0004230:CDS |
| TTATATGGACGACGATTTAG+TGG | 0.463446 | 1_1:-6676373 | Msa0004230:CDS |
| TAGATATGAGGAAGGTGGAG+AGG | 0.465592 | 1_1:+6676139 | None:intergenic |
| ATCGTTTGTTAAGGCTTATG+AGG | 0.466700 | 1_1:-6676398 | Msa0004230:CDS |
| CTGCCACATAGATTATTGTA+AGG | 0.475890 | 1_1:+6676425 | None:intergenic |
| ACCATATCTTATTGTAAAGG+TGG | 0.533013 | 1_1:-6676220 | Msa0004230:CDS |
| AATACCATATCTTATTGTAA+AGG | 0.541676 | 1_1:-6676223 | Msa0004230:CDS |
| GACTGTGTCTACAACGTTGG+TGG | 0.542581 | 1_1:-6676338 | Msa0004230:CDS |
| ATAAGATATGGTATTGTGCT+GGG | 0.555245 | 1_1:+6676231 | None:intergenic |
| TTTGGTGGCAACTTTAATGT+GGG | 0.574460 | 1_1:-6676308 | Msa0004230:CDS |
| TTGAAGTGAAGTTGTGATGC+CGG | 0.583854 | 1_1:+6676166 | None:intergenic |
| AATTTGTTGGCACGGCGTCG+CGG | 0.590331 | 1_1:-6676197 | Msa0004230:CDS |
| AAAGGTGGAATTTGTTGGCA+CGG | 0.594220 | 1_1:-6676205 | Msa0004230:CDS |
| GGTGACTGTGTCTACAACGT+TGG | 0.615144 | 1_1:-6676341 | Msa0004230:CDS |
| TAACCTTACAATAATCTATG+TGG | 0.623412 | 1_1:-6676428 | Msa0004230:CDS |
| GCTGGGAAGTACGAGGATGT+TGG | 0.653136 | 1_1:+6676248 | None:intergenic |
| GTATTGTGCTGGGAAGTACG+AGG | 0.800327 | 1_1:+6676241 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATACCATATCTTATTGTAA+AGG | - | chr1_1:6676369-6676388 | Msa0004230:CDS | 20.0% |
| ! | TAACCTTACAATAATCTATG+TGG | - | chr1_1:6676164-6676183 | Msa0004230:CDS | 25.0% |
| !! | AAGGCTTATGAGGTTTTATA+TGG | - | chr1_1:6676204-6676223 | Msa0004230:CDS | 30.0% |
| ATAAGATATGGTATTGTGCT+GGG | + | chr1_1:6676364-6676383 | None:intergenic | 30.0% | |
| AATAAGATATGGTATTGTGC+TGG | + | chr1_1:6676365-6676384 | None:intergenic | 30.0% | |
| ACCATATCTTATTGTAAAGG+TGG | - | chr1_1:6676372-6676391 | Msa0004230:CDS | 30.0% | |
| TCCACCTTTACAATAAGATA+TGG | + | chr1_1:6676376-6676395 | None:intergenic | 30.0% | |
| ATTGTAAAGGTGGAATTTGT+TGG | - | chr1_1:6676382-6676401 | Msa0004230:CDS | 30.0% | |
| CTGCCACATAGATTATTGTA+AGG | + | chr1_1:6676170-6676189 | None:intergenic | 35.0% | |
| ! | ATCGTTTGTTAAGGCTTATG+AGG | - | chr1_1:6676194-6676213 | Msa0004230:CDS | 35.0% |
| TTATATGGACGACGATTTAG+TGG | - | chr1_1:6676219-6676238 | Msa0004230:CDS | 35.0% | |
| !! | TTTTGGTGGCAACTTTAATG+TGG | - | chr1_1:6676283-6676302 | Msa0004230:CDS | 35.0% |
| ! | TTTGGTGGCAACTTTAATGT+GGG | - | chr1_1:6676284-6676303 | Msa0004230:CDS | 35.0% |
| ! | GGCAGTTACATCGTTTGTTA+AGG | - | chr1_1:6676185-6676204 | Msa0004230:CDS | 40.0% |
| ACGATTTAGTGGTGTGTTTG+TGG | - | chr1_1:6676230-6676249 | Msa0004230:CDS | 40.0% | |
| !!! | AACGTTGGTGGCTTTGATTT+TGG | - | chr1_1:6676266-6676285 | Msa0004230:CDS | 40.0% |
| !! | AAAGGTGGAATTTGTTGGCA+CGG | - | chr1_1:6676387-6676406 | Msa0004230:CDS | 40.0% |
| TTGAAGTGAAGTTGTGATGC+CGG | + | chr1_1:6676429-6676448 | None:intergenic | 40.0% | |
| !!! | GTTGGTGGCTTTGATTTTGG+TGG | - | chr1_1:6676269-6676288 | Msa0004230:CDS | 45.0% |
| TAGATATGAGGAAGGTGGAG+AGG | + | chr1_1:6676456-6676475 | None:intergenic | 45.0% | |
| GGTGACTGTGTCTACAACGT+TGG | - | chr1_1:6676251-6676270 | Msa0004230:CDS | 50.0% | |
| GACTGTGTCTACAACGTTGG+TGG | - | chr1_1:6676254-6676273 | Msa0004230:CDS | 50.0% | |
| ! | GTATTGTGCTGGGAAGTACG+AGG | + | chr1_1:6676354-6676373 | None:intergenic | 50.0% |
| ! | GCTGGGAAGTACGAGGATGT+TGG | + | chr1_1:6676347-6676366 | None:intergenic | 55.0% |
| !! | AATTTGTTGGCACGGCGTCG+CGG | - | chr1_1:6676395-6676414 | Msa0004230:CDS | 55.0% |
| ! | CGGCGTCGCGGATCGCTCGC+CGG | - | chr1_1:6676407-6676426 | Msa0004230:CDS | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_1 | gene | 6676138 | 6676476 | 6676138 | ID=Msa0004230;Name=Msa0004230 |
| chr1_1 | mRNA | 6676138 | 6676476 | 6676138 | ID=Msa0004230-mRNA-1;Parent=Msa0004230;Name=Msa0004230-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|112 |
| chr1_1 | exon | 6676138 | 6676476 | 6676138 | ID=Msa0004230-mRNA-1:exon:2287;Parent=Msa0004230-mRNA-1 |
| chr1_1 | CDS | 6676138 | 6676476 | 6676138 | ID=Msa0004230-mRNA-1:cds;Parent=Msa0004230-mRNA-1 |
| Gene Sequence |
| Protein sequence |
