AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0009770

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0009770 MtrunA17_Chr1g0158811 97.188 320 9 0 1 320 1 320 0.0 635
Msa0009770 MtrunA17_Chr1g0158821 62.539 323 112 6 1 319 1 318 7.75e-135 385
Msa0009770 MtrunA17_Chr3g0127471 63.986 286 92 5 37 317 30 309 1.01e-121 352
Msa0009770 MtrunA17_Chr5g0419391 47.619 252 129 3 69 317 8 259 2.00e-78 240
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0009770 AT5G24400.1 63.306 248 86 3 72 317 81 325 3.44e-106 313
Msa0009770 AT3G49360.1 54.694 245 102 4 78 317 11 251 1.05e-86 261
Msa0009770 AT1G13700.2 50.413 242 116 2 80 317 20 261 1.00e-82 251
Msa0009770 AT1G13700.3 50.413 242 116 2 80 317 20 261 1.00e-82 251
Msa0009770 AT1G13700.1 50.413 242 116 2 80 317 20 261 1.00e-82 251
Msa0009770 AT5G24410.1 53.061 245 106 4 78 317 17 257 4.66e-81 247
Msa0009770 AT5G24420.1 51.837 245 110 4 78 317 11 252 1.84e-78 240

Find 70 sgRNAs with CRISPR-Local

Find 230 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTGCTTCAAAACTGTTTA+AGG 0.136078 1_1:-14616766 Msa0009770:CDS
ATGTTTGAATTTCAGGAAAT+TGG 0.207149 1_1:-14617728 Msa0009770:intron
ATTCTCTCTGATGGTGGTTT+TGG 0.280005 1_1:+14616963 Msa0009760:three_prime_UTR
ATCACTTTATTATGCCTTAG+AGG 0.281551 1_1:+14618149 Msa0009760:three_prime_UTR
AAACCATTGATTGGTCAAAA+TGG 0.309040 1_1:-14617687 Msa0009770:CDS
AGGAGCATGAGATCAAATTT+TGG 0.311450 1_1:+14617080 Msa0009760:three_prime_UTR
TATTTCACTGTTGCTTTGTC+TGG 0.315803 1_1:-14617975 Msa0009770:CDS
TTACTTTCTAAGTGATCCTT+TGG 0.360528 1_1:+14617634 Msa0009760:three_prime_UTR
GGAACATCTTGCTGTTTCAT+TGG 0.385960 1_1:-14618045 Msa0009770:CDS
GTGCACAAAATAAGCTACTT+TGG 0.391008 1_1:+14618243 Msa0009760:three_prime_UTR
TGATCTCATGCTCCTAGGTA+TGG 0.406465 1_1:-14617072 Msa0009770:CDS
TGAAACAACCCTAAGACGTT+TGG 0.412525 1_1:-14617141 Msa0009770:CDS
TTCTCCAAGAGGATCAGAAA+TGG 0.419423 1_1:-14617007 Msa0009770:CDS
AACAAATTCATCAAAGAAAG+AGG 0.421608 1_1:-14617999 Msa0009770:CDS
TTCACTGTTGCTTTGTCTGG+TGG 0.432382 1_1:-14617972 Msa0009770:CDS
GGCATCACTTAAGTGGGAAA+AGG 0.443000 1_1:-14618096 Msa0009770:CDS
TACTTTCTAAGTGATCCTTT+GGG 0.443581 1_1:+14617635 Msa0009760:three_prime_UTR
TAAACGTTGAGAGGAGGAAT+TGG 0.449024 1_1:+14617209 Msa0009760:three_prime_UTR
TAACCCATTTCTGATCCTCT+TGG 0.454962 1_1:+14617003 Msa0009760:three_prime_UTR
GAAAAGGTTTCACCTCTTGA+AGG 0.456560 1_1:-14616816 Msa0009770:CDS
CATGTTGCATCTTTATTCCC+AGG 0.457125 1_1:-14617038 Msa0009770:CDS
CTCTTGAAGGGGAATTGAAA+TGG 0.460789 1_1:-14616803 Msa0009770:CDS
AAAGATGCAACATGTCCATC+TGG 0.461834 1_1:+14617047 Msa0009760:three_prime_UTR
TAAACATCAGCTGCTCCATC+AGG 0.473530 1_1:+14617164 Msa0009760:three_prime_UTR
AATTATAAGCTTGCCAGTGA+TGG 0.484242 1_1:-14617613 Msa0009770:CDS
CAATCAATGGTTTCAGCATA+GGG 0.486339 1_1:+14617697 Msa0009760:three_prime_UTR
GATCTCATGCTCCTAGGTAT+GGG 0.491824 1_1:-14617071 Msa0009770:CDS
TGTCATGTTGCCTGTTGAAA+AGG 0.494449 1_1:-14616832 Msa0009770:CDS
CCAATCAATGGTTTCAGCAT+AGG 0.494503 1_1:+14617696 Msa0009760:three_prime_UTR
AAATAGAGGCATCACTTAAG+TGG 0.496228 1_1:-14618103 Msa0009770:CDS
CGCAGTTTACACTGCACTTG+AGG 0.500062 1_1:-14616868 Msa0009770:CDS
GCATCACTTAAGTGGGAAAA+GGG 0.504570 1_1:-14618095 Msa0009770:CDS
TCTCCAAGAGGATCAGAAAT+GGG 0.506655 1_1:-14617006 Msa0009770:CDS
CAGCTGCTCCATCAGGTGGT+AGG 0.508616 1_1:+14617171 Msa0009760:three_prime_UTR
AAATTTGATCTCATGCTCCT+AGG 0.509138 1_1:-14617077 Msa0009770:CDS
AAAAGGTTTCACCTCTTGAA+GGG 0.515655 1_1:-14616815 Msa0009770:CDS
AGTCACCACCATTGCAACAT+TGG 0.519798 1_1:+14616908 Msa0009760:three_prime_UTR
CCTATGCTGAAACCATTGAT+TGG 0.520392 1_1:-14617696 Msa0009770:CDS
AGCTGCTCCATCAGGTGGTA+GGG 0.529718 1_1:+14617172 Msa0009760:three_prime_UTR
GTGAAAGTAATTCTCTCTGA+TGG 0.540458 1_1:+14616954 Msa0009760:three_prime_UTR
TTGGAAGAAGCATTGATCAC+TGG 0.541052 1_1:+14616927 Msa0009760:three_prime_UTR
GATCATTAATCAAGTACCTA+AGG 0.543945 1_1:-14617950 Msa0009770:intron
ATTAGTCAAAGTTGTTGAGA+AGG 0.549232 1_1:+14618277 Msa0009760:three_prime_UTR
GCAATGGTGGTGACTGGCGC+GGG 0.551673 1_1:-14616900 Msa0009770:CDS
CTCCTAGGTATGGGTCCAGA+TGG 0.553952 1_1:-14617062 Msa0009770:CDS
AATAGAGGCATCACTTAAGT+GGG 0.554935 1_1:-14618102 Msa0009770:CDS
TTGAAATGGTACTTAGACAA+AGG 0.555841 1_1:-14616789 Msa0009770:CDS
TGCTTCTTCCAATGTTGCAA+TGG 0.565044 1_1:-14616916 Msa0009770:CDS
CATTTCAATTCCCCTTCAAG+AGG 0.566687 1_1:+14616804 Msa0009760:three_prime_UTR
GATGATTCCCTACCACCTGA+TGG 0.566852 1_1:-14617179 Msa0009770:CDS
AATGTTGCAATGGTGGTGAC+TGG 0.569664 1_1:-14616906 Msa0009770:CDS
AGGTGGTAGGGAATCATCAA+TGG 0.569674 1_1:+14617184 Msa0009760:three_prime_UTR
TTCTTCCAATGTTGCAATGG+TGG 0.573190 1_1:-14616913 Msa0009770:CDS
AAAATTGATGTCTTTAGTGA+TGG 0.578624 1_1:+14618206 Msa0009760:three_prime_UTR
AAAGGGTTATAAGAATGTTG+AGG 0.580239 1_1:-14618078 Msa0009770:CDS
ATTTCACCAAATAGATGTGT+CGG 0.582027 1_1:-14618128 Msa0009770:CDS
TGCAATGGTGGTGACTGGCG+CGG 0.584998 1_1:-14616901 Msa0009770:CDS
AAAGGTTTCACCTCTTGAAG+GGG 0.598919 1_1:-14616814 Msa0009770:CDS
CTTGGAGAGAAATCCATCAC+TGG 0.601315 1_1:+14617600 Msa0009760:three_prime_UTR
CTTGGAGAAGAGCATGACCT+GGG 0.611662 1_1:+14617021 Msa0009760:three_prime_UTR
AAAGTAATTCTCTCTGATGG+TGG 0.620404 1_1:+14616957 Msa0009760:three_prime_UTR
CAGTGATGGATTTCTCTCCA+AGG 0.622114 1_1:-14617599 Msa0009770:intron
TCTTGGAGAAGAGCATGACC+TGG 0.622800 1_1:+14617020 Msa0009760:three_prime_UTR
TCAATGGCATAAACGTTGAG+AGG 0.637656 1_1:+14617200 Msa0009760:three_prime_UTR
TGATGAGAGAGTTGTCCCAA+AGG 0.638984 1_1:-14617650 Msa0009770:CDS
ATGGCATAAACGTTGAGAGG+AGG 0.640971 1_1:+14617203 Msa0009760:three_prime_UTR
ACATCAGCTGCTCCATCAGG+TGG 0.642046 1_1:+14617167 Msa0009760:three_prime_UTR
AGGTCATGCTCTTCTCCAAG+AGG 0.654284 1_1:-14617018 Msa0009770:CDS
GTCCATCTGGACCCATACCT+AGG 0.668407 1_1:+14617060 Msa0009760:three_prime_UTR
CTCAAGTGCAGTGTAAACTG+CGG 0.695035 1_1:+14616869 Msa0009760:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTAAAAGAAATTGTAATT+AGG - chr1_1:14617218-14617237 Msa0009770:intron 10.0%
!! ATACTAGAAATAAATAATTA+AGG + chr1_1:14617266-14617285 Msa0009760:intron 10.0%
!! ATAGTAACATTTATATTAAA+AGG + chr1_1:14617550-14617569 Msa0009760:intron 10.0%
!! ATTTAAAAGAAATTGTAATT+AGG - chr1_1:14617218-14617237 Msa0009770:intron 10.0%
!! ATACTAGAAATAAATAATTA+AGG + chr1_1:14617266-14617285 Msa0009760:intron 10.0%
!! ATAGTAACATTTATATTAAA+AGG + chr1_1:14617550-14617569 Msa0009760:intron 10.0%
!! AATCACATGAAATTTATATT+AGG + chr1_1:14617175-14617194 Msa0009760:three_prime_UTR 15.0%
!! AATCACATGAAATTTATATT+AGG + chr1_1:14617175-14617194 Msa0009760:three_prime_UTR 15.0%
!!! ATGTGATTTTAATGTTACAT+AGG - chr1_1:14617187-14617206 Msa0009770:CDS 20.0%
!! GAAAATTATGTTTGAATTTC+AGG - chr1_1:14617307-14617326 Msa0009770:intron 20.0%
!!! GAAATTAATCATTTTGATGT+TGG - chr1_1:14617523-14617542 Msa0009770:intron 20.0%
!! ATGATATCGTAATTAGATAA+GGG + chr1_1:14617686-14617705 Msa0009760:three_prime_UTR 20.0%
!! TATGATATCGTAATTAGATA+AGG + chr1_1:14617687-14617706 Msa0009760:three_prime_UTR 20.0%
!! TTATCTAATTACGATATCAT+AGG - chr1_1:14617686-14617705 Msa0009770:CDS 20.0%
!!! ATCAAATTTTGGTAATTCAT+TGG + chr1_1:14617954-14617973 Msa0009760:three_prime_UTR 20.0%
!!! ATGTGATTTTAATGTTACAT+AGG - chr1_1:14617187-14617206 Msa0009770:CDS 20.0%
!! GAAAATTATGTTTGAATTTC+AGG - chr1_1:14617307-14617326 Msa0009770:intron 20.0%
!!! GAAATTAATCATTTTGATGT+TGG - chr1_1:14617523-14617542 Msa0009770:intron 20.0%
!! ATGATATCGTAATTAGATAA+GGG + chr1_1:14617686-14617705 Msa0009760:three_prime_UTR 20.0%
!! TATGATATCGTAATTAGATA+AGG + chr1_1:14617687-14617706 Msa0009760:three_prime_UTR 20.0%
!! TTATCTAATTACGATATCAT+AGG - chr1_1:14617686-14617705 Msa0009770:CDS 20.0%
!!! ATCAAATTTTGGTAATTCAT+TGG + chr1_1:14617954-14617973 Msa0009760:three_prime_UTR 20.0%
! AAAATTGATGTCTTTAGTGA+TGG + chr1_1:14616839-14616858 Msa0009760:three_prime_UTR 25.0%
! AACAAATTCATCAAAGAAAG+AGG - chr1_1:14617043-14617062 Msa0009770:CDS 25.0%
! AGGATTTCAAGAACAAAAAA+TGG - chr1_1:14617238-14617257 Msa0009770:intron 25.0%
! ATGTTTGAATTTCAGGAAAT+TGG - chr1_1:14617314-14617333 Msa0009770:intron 25.0%
! AAATCGAACAACTAATCAAA+TGG + chr1_1:14617644-14617663 Msa0009760:three_prime_UTR 25.0%
! GAATTAACAATCATCAAACT+TGG + chr1_1:14617725-14617744 Msa0009760:intron 25.0%
! TCGACAATAAGATTTATTGA+CGG + chr1_1:14617748-14617767 Msa0009760:intron 25.0%
! AAAATTGATGTCTTTAGTGA+TGG + chr1_1:14616839-14616858 Msa0009760:three_prime_UTR 25.0%
! AACAAATTCATCAAAGAAAG+AGG - chr1_1:14617043-14617062 Msa0009770:CDS 25.0%
! AGGATTTCAAGAACAAAAAA+TGG - chr1_1:14617238-14617257 Msa0009770:intron 25.0%
! ATGTTTGAATTTCAGGAAAT+TGG - chr1_1:14617314-14617333 Msa0009770:intron 25.0%
! AAATCGAACAACTAATCAAA+TGG + chr1_1:14617644-14617663 Msa0009760:three_prime_UTR 25.0%
! GAATTAACAATCATCAAACT+TGG + chr1_1:14617725-14617744 Msa0009760:intron 25.0%
! TCGACAATAAGATTTATTGA+CGG + chr1_1:14617748-14617767 Msa0009760:intron 25.0%
ATTAGTCAAAGTTGTTGAGA+AGG + chr1_1:14616768-14616787 Msa0009760:three_prime_UTR 30.0%
! TCAATTTTACCAACCCAAAT+TGG - chr1_1:14616851-14616870 Msa0009770:CDS 30.0%
! CAATTTTACCAACCCAAATT+GGG - chr1_1:14616852-14616871 Msa0009770:CDS 30.0%
! ACCAATGTTTTTCCCAATTT+GGG + chr1_1:14616867-14616886 Msa0009760:three_prime_UTR 30.0%
! AACCAATGTTTTTCCCAATT+TGG + chr1_1:14616868-14616887 Msa0009760:three_prime_UTR 30.0%
ATCACTTTATTATGCCTTAG+AGG + chr1_1:14616896-14616915 Msa0009760:three_prime_UTR 30.0%
ATTTCACCAAATAGATGTGT+CGG - chr1_1:14616914-14616933 Msa0009770:CDS 30.0%
! ATTTTTCCGACACATCTATT+TGG + chr1_1:14616923-14616942 Msa0009760:three_prime_UTR 30.0%
AAAGGGTTATAAGAATGTTG+AGG - chr1_1:14616964-14616983 Msa0009770:CDS 30.0%
!!! GAATGTTGAGGTTTTTAGTA+AGG - chr1_1:14616976-14616995 Msa0009770:CDS 30.0%
GATCATTAATCAAGTACCTA+AGG - chr1_1:14617092-14617111 Msa0009770:CDS 30.0%
TGTTAAGAATTACACACCTT+AGG + chr1_1:14617111-14617130 Msa0009760:three_prime_UTR 30.0%
!! GATTTTAATGTTACATAGGC+TGG - chr1_1:14617191-14617210 Msa0009770:CDS 30.0%
! AAACCATTGATTGGTCAAAA+TGG - chr1_1:14617355-14617374 Msa0009770:intron 30.0%
TACTTTCTAAGTGATCCTTT+GGG + chr1_1:14617410-14617429 Msa0009760:intron 30.0%
TTACTTTCTAAGTGATCCTT+TGG + chr1_1:14617411-14617430 Msa0009760:intron 30.0%
CGACAATAAGATTTATTGAC+GGG + chr1_1:14617747-14617766 Msa0009760:intron 30.0%
ATTTCCATTATTCCTTTGTC+AGG - chr1_1:14617808-14617827 Msa0009770:intron 30.0%
TTGAAATGGTACTTAGACAA+AGG - chr1_1:14618253-14618272 Msa0009770:CDS 30.0%
ATTAGTCAAAGTTGTTGAGA+AGG + chr1_1:14616768-14616787 Msa0009760:three_prime_UTR 30.0%
! TCAATTTTACCAACCCAAAT+TGG - chr1_1:14616851-14616870 Msa0009770:CDS 30.0%
! CAATTTTACCAACCCAAATT+GGG - chr1_1:14616852-14616871 Msa0009770:CDS 30.0%
! ACCAATGTTTTTCCCAATTT+GGG + chr1_1:14616867-14616886 Msa0009760:three_prime_UTR 30.0%
! AACCAATGTTTTTCCCAATT+TGG + chr1_1:14616868-14616887 Msa0009760:three_prime_UTR 30.0%
ATCACTTTATTATGCCTTAG+AGG + chr1_1:14616896-14616915 Msa0009760:three_prime_UTR 30.0%
ATTTCACCAAATAGATGTGT+CGG - chr1_1:14616914-14616933 Msa0009770:CDS 30.0%
! ATTTTTCCGACACATCTATT+TGG + chr1_1:14616923-14616942 Msa0009760:three_prime_UTR 30.0%
AAAGGGTTATAAGAATGTTG+AGG - chr1_1:14616964-14616983 Msa0009770:CDS 30.0%
!!! GAATGTTGAGGTTTTTAGTA+AGG - chr1_1:14616976-14616995 Msa0009770:CDS 30.0%
GATCATTAATCAAGTACCTA+AGG - chr1_1:14617092-14617111 Msa0009770:CDS 30.0%
TGTTAAGAATTACACACCTT+AGG + chr1_1:14617111-14617130 Msa0009760:three_prime_UTR 30.0%
!! GATTTTAATGTTACATAGGC+TGG - chr1_1:14617191-14617210 Msa0009770:CDS 30.0%
! AAACCATTGATTGGTCAAAA+TGG - chr1_1:14617355-14617374 Msa0009770:intron 30.0%
TACTTTCTAAGTGATCCTTT+GGG + chr1_1:14617410-14617429 Msa0009760:intron 30.0%
TTACTTTCTAAGTGATCCTT+TGG + chr1_1:14617411-14617430 Msa0009760:intron 30.0%
CGACAATAAGATTTATTGAC+GGG + chr1_1:14617747-14617766 Msa0009760:intron 30.0%
ATTTCCATTATTCCTTTGTC+AGG - chr1_1:14617808-14617827 Msa0009770:intron 30.0%
TTGAAATGGTACTTAGACAA+AGG - chr1_1:14618253-14618272 Msa0009770:CDS 30.0%
GTGCACAAAATAAGCTACTT+TGG + chr1_1:14616802-14616821 Msa0009760:three_prime_UTR 35.0%
! ATGTTTTTCCCAATTTGGGT+TGG + chr1_1:14616863-14616882 Msa0009760:three_prime_UTR 35.0%
ACCCAAATTGGGAAAAACAT+TGG - chr1_1:14616863-14616882 Msa0009770:CDS 35.0%
!!! ACATTGGTTTTCAACCTCTA+AGG - chr1_1:14616879-14616898 Msa0009770:CDS 35.0%
TAGATGTGTCGGAAAAATAG+AGG - chr1_1:14616925-14616944 Msa0009770:CDS 35.0%
!! AAATAGAGGCATCACTTAAG+TGG - chr1_1:14616939-14616958 Msa0009770:CDS 35.0%
!! AATAGAGGCATCACTTAAGT+GGG - chr1_1:14616940-14616959 Msa0009770:CDS 35.0%
!! TATTTCACTGTTGCTTTGTC+TGG - chr1_1:14617067-14617086 Msa0009770:CDS 35.0%
CAATCAATGGTTTCAGCATA+GGG + chr1_1:14617348-14617367 Msa0009760:intron 35.0%
!!! ATGCCATTTTGACCAATCAA+TGG + chr1_1:14617361-14617380 Msa0009760:intron 35.0%
! GTCAAAATGGCATGTTTTCT+GGG - chr1_1:14617368-14617387 Msa0009770:intron 35.0%
AATTATAAGCTTGCCAGTGA+TGG - chr1_1:14617429-14617448 Msa0009770:intron 35.0%
TTCAAGCAAAACAATCAACC+TGG + chr1_1:14617484-14617503 Msa0009760:intron 35.0%
TGCTTGAAGAAGAATGATGA+TGG - chr1_1:14617496-14617515 Msa0009770:intron 35.0%
CTTATTGTCGATCTTTAGTG+AGG - chr1_1:14617757-14617776 Msa0009770:intron 35.0%
! GCTTTTAACCAAACGTCTTA+GGG + chr1_1:14617912-14617931 Msa0009760:intron 35.0%
! TGCTTTTAACCAAACGTCTT+AGG + chr1_1:14617913-14617932 Msa0009760:intron 35.0%
!! AGGAGCATGAGATCAAATTT+TGG + chr1_1:14617965-14617984 Msa0009760:three_prime_UTR 35.0%
AAATTTGATCTCATGCTCCT+AGG - chr1_1:14617965-14617984 Msa0009770:CDS 35.0%
AAAGTAATTCTCTCTGATGG+TGG + chr1_1:14618088-14618107 Msa0009760:three_prime_UTR 35.0%
GTGAAAGTAATTCTCTCTGA+TGG + chr1_1:14618091-14618110 Msa0009760:three_prime_UTR 35.0%
AAAAGGTTTCACCTCTTGAA+GGG - chr1_1:14618227-14618246 Msa0009770:CDS 35.0%
! TGCTGCTTCAAAACTGTTTA+AGG - chr1_1:14618276-14618295 Msa0009770:CDS 35.0%
GTGCACAAAATAAGCTACTT+TGG + chr1_1:14616802-14616821 Msa0009760:three_prime_UTR 35.0%
! ATGTTTTTCCCAATTTGGGT+TGG + chr1_1:14616863-14616882 Msa0009760:three_prime_UTR 35.0%
ACCCAAATTGGGAAAAACAT+TGG - chr1_1:14616863-14616882 Msa0009770:CDS 35.0%
!!! ACATTGGTTTTCAACCTCTA+AGG - chr1_1:14616879-14616898 Msa0009770:CDS 35.0%
TAGATGTGTCGGAAAAATAG+AGG - chr1_1:14616925-14616944 Msa0009770:CDS 35.0%
!! AAATAGAGGCATCACTTAAG+TGG - chr1_1:14616939-14616958 Msa0009770:CDS 35.0%
!! AATAGAGGCATCACTTAAGT+GGG - chr1_1:14616940-14616959 Msa0009770:CDS 35.0%
!! TATTTCACTGTTGCTTTGTC+TGG - chr1_1:14617067-14617086 Msa0009770:CDS 35.0%
CAATCAATGGTTTCAGCATA+GGG + chr1_1:14617348-14617367 Msa0009760:intron 35.0%
!!! ATGCCATTTTGACCAATCAA+TGG + chr1_1:14617361-14617380 Msa0009760:intron 35.0%
! GTCAAAATGGCATGTTTTCT+GGG - chr1_1:14617368-14617387 Msa0009770:intron 35.0%
AATTATAAGCTTGCCAGTGA+TGG - chr1_1:14617429-14617448 Msa0009770:intron 35.0%
TTCAAGCAAAACAATCAACC+TGG + chr1_1:14617484-14617503 Msa0009760:intron 35.0%
TGCTTGAAGAAGAATGATGA+TGG - chr1_1:14617496-14617515 Msa0009770:intron 35.0%
CTTATTGTCGATCTTTAGTG+AGG - chr1_1:14617757-14617776 Msa0009770:intron 35.0%
! GCTTTTAACCAAACGTCTTA+GGG + chr1_1:14617912-14617931 Msa0009760:intron 35.0%
! TGCTTTTAACCAAACGTCTT+AGG + chr1_1:14617913-14617932 Msa0009760:intron 35.0%
!! AGGAGCATGAGATCAAATTT+TGG + chr1_1:14617965-14617984 Msa0009760:three_prime_UTR 35.0%
AAATTTGATCTCATGCTCCT+AGG - chr1_1:14617965-14617984 Msa0009770:CDS 35.0%
AAAGTAATTCTCTCTGATGG+TGG + chr1_1:14618088-14618107 Msa0009760:three_prime_UTR 35.0%
GTGAAAGTAATTCTCTCTGA+TGG + chr1_1:14618091-14618110 Msa0009760:three_prime_UTR 35.0%
AAAAGGTTTCACCTCTTGAA+GGG - chr1_1:14618227-14618246 Msa0009770:CDS 35.0%
! TGCTGCTTCAAAACTGTTTA+AGG - chr1_1:14618276-14618295 Msa0009770:CDS 35.0%
GCATCACTTAAGTGGGAAAA+GGG - chr1_1:14616947-14616966 Msa0009770:CDS 40.0%
!! GGAACATCTTGCTGTTTCAT+TGG - chr1_1:14616997-14617016 Msa0009770:CDS 40.0%
CCAATCAATGGTTTCAGCAT+AGG + chr1_1:14617349-14617368 Msa0009760:intron 40.0%
CCTATGCTGAAACCATTGAT+TGG - chr1_1:14617346-14617365 Msa0009770:intron 40.0%
! GGTCAAAATGGCATGTTTTC+TGG - chr1_1:14617367-14617386 Msa0009770:intron 40.0%
GGCACATGAGTTAATTACCT+TGG + chr1_1:14617463-14617482 Msa0009760:intron 40.0%
AGGTAATTAACTCATGTGCC+AGG - chr1_1:14617463-14617482 Msa0009770:intron 40.0%
TACTAGCATATCGTGACATG+TGG - chr1_1:14617598-14617617 Msa0009770:intron 40.0%
ATTACGATATCATAGGTGCG+AGG - chr1_1:14617693-14617712 Msa0009770:CDS 40.0%
TAAACGTTGAGAGGAGGAAT+TGG + chr1_1:14617836-14617855 Msa0009760:intron 40.0%
TCAATGGCATAAACGTTGAG+AGG + chr1_1:14617845-14617864 Msa0009760:intron 40.0%
TGAAACAACCCTAAGACGTT+TGG - chr1_1:14617901-14617920 Msa0009770:intron 40.0%
AAAGATGCAACATGTCCATC+TGG + chr1_1:14617998-14618017 Msa0009760:three_prime_UTR 40.0%
CATGTTGCATCTTTATTCCC+AGG - chr1_1:14618004-14618023 Msa0009770:CDS 40.0%
TTCTCCAAGAGGATCAGAAA+TGG - chr1_1:14618035-14618054 Msa0009770:CDS 40.0%
TCTCCAAGAGGATCAGAAAT+GGG - chr1_1:14618036-14618055 Msa0009770:CDS 40.0%
TAACCCATTTCTGATCCTCT+TGG + chr1_1:14618042-14618061 Msa0009760:three_prime_UTR 40.0%
!!! ATTCTCTCTGATGGTGGTTT+TGG + chr1_1:14618082-14618101 Msa0009760:three_prime_UTR 40.0%
!! TTGGAAGAAGCATTGATCAC+TGG + chr1_1:14618118-14618137 Msa0009760:three_prime_UTR 40.0%
TGCTTCTTCCAATGTTGCAA+TGG - chr1_1:14618126-14618145 Msa0009770:CDS 40.0%
TTCTTCCAATGTTGCAATGG+TGG - chr1_1:14618129-14618148 Msa0009770:CDS 40.0%
TGTCATGTTGCCTGTTGAAA+AGG - chr1_1:14618210-14618229 Msa0009770:CDS 40.0%
! AGAGGTGAAACCTTTTCAAC+AGG + chr1_1:14618223-14618242 Msa0009760:three_prime_UTR 40.0%
GAAAAGGTTTCACCTCTTGA+AGG - chr1_1:14618226-14618245 Msa0009770:CDS 40.0%
AAAGGTTTCACCTCTTGAAG+GGG - chr1_1:14618228-14618247 Msa0009770:CDS 40.0%
CATTTCAATTCCCCTTCAAG+AGG + chr1_1:14618241-14618260 Msa0009760:three_prime_UTR 40.0%
CTCTTGAAGGGGAATTGAAA+TGG - chr1_1:14618239-14618258 Msa0009770:CDS 40.0%
GCATCACTTAAGTGGGAAAA+GGG - chr1_1:14616947-14616966 Msa0009770:CDS 40.0%
!! GGAACATCTTGCTGTTTCAT+TGG - chr1_1:14616997-14617016 Msa0009770:CDS 40.0%
CCAATCAATGGTTTCAGCAT+AGG + chr1_1:14617349-14617368 Msa0009760:intron 40.0%
CCTATGCTGAAACCATTGAT+TGG - chr1_1:14617346-14617365 Msa0009770:intron 40.0%
! GGTCAAAATGGCATGTTTTC+TGG - chr1_1:14617367-14617386 Msa0009770:intron 40.0%
GGCACATGAGTTAATTACCT+TGG + chr1_1:14617463-14617482 Msa0009760:intron 40.0%
AGGTAATTAACTCATGTGCC+AGG - chr1_1:14617463-14617482 Msa0009770:intron 40.0%
TACTAGCATATCGTGACATG+TGG - chr1_1:14617598-14617617 Msa0009770:intron 40.0%
ATTACGATATCATAGGTGCG+AGG - chr1_1:14617693-14617712 Msa0009770:CDS 40.0%
TAAACGTTGAGAGGAGGAAT+TGG + chr1_1:14617836-14617855 Msa0009760:intron 40.0%
TCAATGGCATAAACGTTGAG+AGG + chr1_1:14617845-14617864 Msa0009760:intron 40.0%
TGAAACAACCCTAAGACGTT+TGG - chr1_1:14617901-14617920 Msa0009770:intron 40.0%
AAAGATGCAACATGTCCATC+TGG + chr1_1:14617998-14618017 Msa0009760:three_prime_UTR 40.0%
CATGTTGCATCTTTATTCCC+AGG - chr1_1:14618004-14618023 Msa0009770:CDS 40.0%
TTCTCCAAGAGGATCAGAAA+TGG - chr1_1:14618035-14618054 Msa0009770:CDS 40.0%
TCTCCAAGAGGATCAGAAAT+GGG - chr1_1:14618036-14618055 Msa0009770:CDS 40.0%
TAACCCATTTCTGATCCTCT+TGG + chr1_1:14618042-14618061 Msa0009760:three_prime_UTR 40.0%
!!! ATTCTCTCTGATGGTGGTTT+TGG + chr1_1:14618082-14618101 Msa0009760:three_prime_UTR 40.0%
!! TTGGAAGAAGCATTGATCAC+TGG + chr1_1:14618118-14618137 Msa0009760:three_prime_UTR 40.0%
TGCTTCTTCCAATGTTGCAA+TGG - chr1_1:14618126-14618145 Msa0009770:CDS 40.0%
TTCTTCCAATGTTGCAATGG+TGG - chr1_1:14618129-14618148 Msa0009770:CDS 40.0%
TGTCATGTTGCCTGTTGAAA+AGG - chr1_1:14618210-14618229 Msa0009770:CDS 40.0%
! AGAGGTGAAACCTTTTCAAC+AGG + chr1_1:14618223-14618242 Msa0009760:three_prime_UTR 40.0%
GAAAAGGTTTCACCTCTTGA+AGG - chr1_1:14618226-14618245 Msa0009770:CDS 40.0%
AAAGGTTTCACCTCTTGAAG+GGG - chr1_1:14618228-14618247 Msa0009770:CDS 40.0%
CATTTCAATTCCCCTTCAAG+AGG + chr1_1:14618241-14618260 Msa0009760:three_prime_UTR 40.0%
CTCTTGAAGGGGAATTGAAA+TGG - chr1_1:14618239-14618258 Msa0009770:CDS 40.0%
GGCATCACTTAAGTGGGAAA+AGG - chr1_1:14616946-14616965 Msa0009770:CDS 45.0%
!! TTCACTGTTGCTTTGTCTGG+TGG - chr1_1:14617070-14617089 Msa0009770:CDS 45.0%
TGATGAGAGAGTTGTCCCAA+AGG - chr1_1:14617392-14617411 Msa0009770:intron 45.0%
CTTGGAGAGAAATCCATCAC+TGG + chr1_1:14617445-14617464 Msa0009760:intron 45.0%
!! CAGTGATGGATTTCTCTCCA+AGG - chr1_1:14617443-14617462 Msa0009770:intron 45.0%
GGCACCTGACAAAGGAATAA+TGG + chr1_1:14617815-14617834 Msa0009760:intron 45.0%
ATGGCATAAACGTTGAGAGG+AGG + chr1_1:14617842-14617861 Msa0009760:intron 45.0%
AGGTGGTAGGGAATCATCAA+TGG + chr1_1:14617861-14617880 Msa0009760:intron 45.0%
TAAACATCAGCTGCTCCATC+AGG + chr1_1:14617881-14617900 Msa0009760:intron 45.0%
TGATCTCATGCTCCTAGGTA+TGG - chr1_1:14617970-14617989 Msa0009770:CDS 45.0%
GATCTCATGCTCCTAGGTAT+GGG - chr1_1:14617971-14617990 Msa0009770:CDS 45.0%
AGTCACCACCATTGCAACAT+TGG + chr1_1:14618137-14618156 Msa0009760:three_prime_UTR 45.0%
! AATGTTGCAATGGTGGTGAC+TGG - chr1_1:14618136-14618155 Msa0009770:CDS 45.0%
CTCAAGTGCAGTGTAAACTG+CGG + chr1_1:14618176-14618195 Msa0009760:three_prime_UTR 45.0%
GGCATCACTTAAGTGGGAAA+AGG - chr1_1:14616946-14616965 Msa0009770:CDS 45.0%
!! TTCACTGTTGCTTTGTCTGG+TGG - chr1_1:14617070-14617089 Msa0009770:CDS 45.0%
TGATGAGAGAGTTGTCCCAA+AGG - chr1_1:14617392-14617411 Msa0009770:intron 45.0%
CTTGGAGAGAAATCCATCAC+TGG + chr1_1:14617445-14617464 Msa0009760:intron 45.0%
!! CAGTGATGGATTTCTCTCCA+AGG - chr1_1:14617443-14617462 Msa0009770:intron 45.0%
GGCACCTGACAAAGGAATAA+TGG + chr1_1:14617815-14617834 Msa0009760:intron 45.0%
ATGGCATAAACGTTGAGAGG+AGG + chr1_1:14617842-14617861 Msa0009760:intron 45.0%
AGGTGGTAGGGAATCATCAA+TGG + chr1_1:14617861-14617880 Msa0009760:intron 45.0%
TAAACATCAGCTGCTCCATC+AGG + chr1_1:14617881-14617900 Msa0009760:intron 45.0%
TGATCTCATGCTCCTAGGTA+TGG - chr1_1:14617970-14617989 Msa0009770:CDS 45.0%
GATCTCATGCTCCTAGGTAT+GGG - chr1_1:14617971-14617990 Msa0009770:CDS 45.0%
AGTCACCACCATTGCAACAT+TGG + chr1_1:14618137-14618156 Msa0009760:three_prime_UTR 45.0%
! AATGTTGCAATGGTGGTGAC+TGG - chr1_1:14618136-14618155 Msa0009770:CDS 45.0%
CTCAAGTGCAGTGTAAACTG+CGG + chr1_1:14618176-14618195 Msa0009760:three_prime_UTR 45.0%
GAGGAATTGGCACCTGACAA+AGG + chr1_1:14617823-14617842 Msa0009760:intron 50.0%
GATGATTCCCTACCACCTGA+TGG - chr1_1:14617863-14617882 Msa0009770:intron 50.0%
! CTTGGAGAAGAGCATGACCT+GGG + chr1_1:14618024-14618043 Msa0009760:three_prime_UTR 50.0%
! TCTTGGAGAAGAGCATGACC+TGG + chr1_1:14618025-14618044 Msa0009760:three_prime_UTR 50.0%
AGGTCATGCTCTTCTCCAAG+AGG - chr1_1:14618024-14618043 Msa0009770:CDS 50.0%
!!! GTGGTTTTGGTGAGTCAGTG+AGG + chr1_1:14618069-14618088 Msa0009760:three_prime_UTR 50.0%
CGCAGTTTACACTGCACTTG+AGG - chr1_1:14618174-14618193 Msa0009770:CDS 50.0%
GAGGAATTGGCACCTGACAA+AGG + chr1_1:14617823-14617842 Msa0009760:intron 50.0%
GATGATTCCCTACCACCTGA+TGG - chr1_1:14617863-14617882 Msa0009770:intron 50.0%
! CTTGGAGAAGAGCATGACCT+GGG + chr1_1:14618024-14618043 Msa0009760:three_prime_UTR 50.0%
! TCTTGGAGAAGAGCATGACC+TGG + chr1_1:14618025-14618044 Msa0009760:three_prime_UTR 50.0%
AGGTCATGCTCTTCTCCAAG+AGG - chr1_1:14618024-14618043 Msa0009770:CDS 50.0%
!!! GTGGTTTTGGTGAGTCAGTG+AGG + chr1_1:14618069-14618088 Msa0009760:three_prime_UTR 50.0%
CGCAGTTTACACTGCACTTG+AGG - chr1_1:14618174-14618193 Msa0009770:CDS 50.0%
AGCTGCTCCATCAGGTGGTA+GGG + chr1_1:14617873-14617892 Msa0009760:intron 55.0%
ACATCAGCTGCTCCATCAGG+TGG + chr1_1:14617878-14617897 Msa0009760:intron 55.0%
CTCCTAGGTATGGGTCCAGA+TGG - chr1_1:14617980-14617999 Msa0009770:CDS 55.0%
GTCCATCTGGACCCATACCT+AGG + chr1_1:14617985-14618004 Msa0009760:three_prime_UTR 55.0%
AGCTGCTCCATCAGGTGGTA+GGG + chr1_1:14617873-14617892 Msa0009760:intron 55.0%
ACATCAGCTGCTCCATCAGG+TGG + chr1_1:14617878-14617897 Msa0009760:intron 55.0%
CTCCTAGGTATGGGTCCAGA+TGG - chr1_1:14617980-14617999 Msa0009770:CDS 55.0%
GTCCATCTGGACCCATACCT+AGG + chr1_1:14617985-14618004 Msa0009760:three_prime_UTR 55.0%
CAGCTGCTCCATCAGGTGGT+AGG + chr1_1:14617874-14617893 Msa0009760:intron 60.0%
! TGCAATGGTGGTGACTGGCG+CGG - chr1_1:14618141-14618160 Msa0009770:CDS 60.0%
CAGCTGCTCCATCAGGTGGT+AGG + chr1_1:14617874-14617893 Msa0009760:intron 60.0%
! TGCAATGGTGGTGACTGGCG+CGG - chr1_1:14618141-14618160 Msa0009770:CDS 60.0%
! GCAATGGTGGTGACTGGCGC+GGG - chr1_1:14618142-14618161 Msa0009770:CDS 65.0%
! GCAATGGTGGTGACTGGCGC+GGG - chr1_1:14618142-14618161 Msa0009770:CDS 65.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 14616761 14618303 14616761 ID=Msa0009770;Name=Msa0009770
chr1_1 mRNA 14616761 14618303 14616761 ID=Msa0009770-mRNA-1;Parent=Msa0009770;Name=Msa0009770-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0|0|0|1|1|1|3|0|320
chr1_1 exon 14617951 14618303 14617951 ID=Msa0009770-mRNA-1:exon:5416;Parent=Msa0009770-mRNA-1
chr1_1 exon 14617600 14617735 14617600 ID=Msa0009770-mRNA-1:exon:5415;Parent=Msa0009770-mRNA-1
chr1_1 exon 14616761 14617234 14616761 ID=Msa0009770-mRNA-1:exon:5414;Parent=Msa0009770-mRNA-1
chr1_1 CDS 14617951 14618303 14617951 ID=Msa0009770-mRNA-1:cds;Parent=Msa0009770-mRNA-1
chr1_1 CDS 14617600 14617735 14617600 ID=Msa0009770-mRNA-1:cds;Parent=Msa0009770-mRNA-1
chr1_1 CDS 14616761 14617234 14616761 ID=Msa0009770-mRNA-1:cds;Parent=Msa0009770-mRNA-1
Gene Sequence

>Msa0009770

ATGGCCTTCTCAACAACTTTGACTAATTCATGCACTCTCCAAAGTAGCTTATTTTGTGCACAAAAATCAAATTCACCATCACTAAAGACATCAATTTTACCAACCCAAATTGGGAAAAACATTGGTTTTCAACCTCTAAGGCATAATAAAGTGATTTCACCAAATAGATGTGTCGGAAAAATAGAGGCATCACTTAAGTGGGAAAAGGGTTATAAGAATGTTGAGGTTTTTAGTAAGGAACATCTTGCTGTTTCATTGGCTTATGATGTTGCTCAACTTTCTAACAAATTCATCAAAGAAAGAGGTTATTTCACTGTTGCTTTGTCTGGTGGATCATTAATCAAGTACCTAAGGAAATTGGTTGAATCTCCCTATGCTGAAACCATTGATTGGTCAAAATGGCATGTTTTCTGGGTTGATGAGAGAGTTGTCCCAAAGGATCACTTAGAAAGTAATTATAAGCTTGCCAGTGATGGATTTCTCTCCAAGGTGCCAATTCCTCCTCTCAACGTTTATGCCATTGATGATTCCCTACCACCTGATGGAGCAGCTGATGTTTATGAAACAACCCTAAGACGTTTGGTTAAAAGCAATGTGATATCTACATCAACCAATGAATTACCAAAATTTGATCTCATGCTCCTAGGTATGGGTCCAGATGGACATGTTGCATCTTTATTCCCAGGTCATGCTCTTCTCCAAGAGGATCAGAAATGGGTTACTTTCCTCACTGACTCACCAAAACCACCATCAGAGAGAATTACTTTCACTTTTCCAGTGATCAATGCTTCTTCCAATGTTGCAATGGTGGTGACTGGCGCGGGTAAATCGACCGCAGTTTACACTGCACTTGAGGATGAGAAGAAAACTGTCATGTTGCCTGTTGAAAAGGTTTCACCTCTTGAAGGGGAATTGAAATGGTACTTAGACAAAGGTGCTGCTTCAAAACTGTTTAAGGAGTAA
Protein sequence

>Msa0009770

MAFSTTLTNSCTLQSSLFCAQKSNSPSLKTSILPTQIGKNIGFQPLRHNKVISPNRCVGKIEASLKWEKGYKNVEVFSKEHLAVSLAYDVAQLSNKFIKERGYFTVALSGGSLIKYLRKLVESPYAETIDWSKWHVFWVDERVVPKDHLESNYKLASDGFLSKVPIPPLNVYAIDDSLPPDGAADVYETTLRRLVKSNVISTSTNELPKFDLMLLGMGPDGHVASLFPGHALLQEDQKWVTFLTDSPKPPSERITFTFPVINASSNVAMVVTGAGKSTAVYTALEDEKKTVMLPVEKVSPLEGELKWYLDKGAASKLFKE*