AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0150580

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0150580 MtrunA17_Chr1g0158811 97.812 320 7 0 1 320 1 320 0.0 643
Msa0150580 MtrunA17_Chr1g0158821 62.229 323 113 6 1 319 1 318 9.07e-134 382
Msa0150580 MtrunA17_Chr3g0127471 63.986 286 92 5 37 317 30 309 1.41e-121 351
Msa0150580 MtrunA17_Chr5g0419391 48.016 252 128 3 69 317 8 259 5.50e-79 241
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0150580 AT5G24400.1 63.306 248 86 3 72 317 81 325 5.50e-106 313
Msa0150580 AT3G49360.1 54.286 245 103 4 78 317 11 251 2.42e-86 260
Msa0150580 AT1G13700.2 50.413 242 116 2 80 317 20 261 1.39e-82 251
Msa0150580 AT1G13700.3 50.413 242 116 2 80 317 20 261 1.39e-82 251
Msa0150580 AT1G13700.1 50.413 242 116 2 80 317 20 261 1.39e-82 251
Msa0150580 AT5G24410.1 52.653 245 107 4 78 317 17 257 1.51e-80 245
Msa0150580 AT5G24420.1 51.837 245 110 4 78 317 11 252 2.12e-78 239

Find 70 sgRNAs with CRISPR-Local

Find 234 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTGCTTCAAAACTGTTTA+AGG 0.136078 1_4:-12198133 Msa0150580:CDS
ATGTTTGAATTTCAGGAAAT+TGG 0.207149 1_4:-12199095 Msa0150580:intron
ATTCTCTCTGATGGTGGTTT+TGG 0.280005 1_4:+12198330 Msa0150570:three_prime_UTR
AACACTTTATTATGCCTTAG+AGG 0.288583 1_4:+12199538 Msa0150570:three_prime_UTR
AAACCATAGATTGGACAAAA+TGG 0.303574 1_4:-12199054 Msa0150580:CDS
AGGAGCATGAGATCAAATTT+TGG 0.311450 1_4:+12198447 Msa0150570:three_prime_UTR
TATTTCACTGTTGCTTTGTC+TGG 0.315803 1_4:-12199364 Msa0150580:CDS
ATTGATGTCTTTAGTGATAG+TGG 0.318425 1_4:+12199598 Msa0150570:three_prime_UTR
TTACTTTCTAAGTGATCCTT+TGG 0.360528 1_4:+12199001 Msa0150570:three_prime_UTR
GGAACATCTTGCTGTTTCAT+TGG 0.385960 1_4:-12199434 Msa0150580:CDS
GTGCACAAAATAAGCTACTT+TGG 0.391008 1_4:+12199632 Msa0150570:three_prime_UTR
TGATCTCATGCTCCTAGGTA+TGG 0.406465 1_4:-12198439 Msa0150580:CDS
TGAAACAACCCTAAGACGTT+TGG 0.412525 1_4:-12198508 Msa0150580:CDS
TTCTCCAAGAGGATCAGAAA+TGG 0.419423 1_4:-12198374 Msa0150580:CDS
AACAAATTCATCAAAGAAAG+AGG 0.421608 1_4:-12199388 Msa0150580:CDS
TTCACTGTTGCTTTGTCTGG+TGG 0.432382 1_4:-12199361 Msa0150580:CDS
GGCATCACTTAAGTGGGAAA+AGG 0.443000 1_4:-12199485 Msa0150580:CDS
TACTTTCTAAGTGATCCTTT+GGG 0.443581 1_4:+12199002 Msa0150570:three_prime_UTR
TAAACGTTGAGAGGAGGAAT+TGG 0.449024 1_4:+12198576 Msa0150570:three_prime_UTR
TAACCCATTTCTGATCCTCT+TGG 0.454962 1_4:+12198370 Msa0150570:three_prime_UTR
GAAAAGGTTTCACCTCTTGA+AGG 0.456560 1_4:-12198183 Msa0150580:CDS
CATGTTGCATCTTTATTCCC+AGG 0.457125 1_4:-12198405 Msa0150580:CDS
CTCTTGAAGGGGAATTGAAA+TGG 0.460789 1_4:-12198170 Msa0150580:CDS
AAAGATGCAACATGTCCATC+TGG 0.461834 1_4:+12198414 Msa0150570:three_prime_UTR
TAAACATCAGCTGCTCCATC+AGG 0.473530 1_4:+12198531 Msa0150570:three_prime_UTR
CCAATCTATGGTTTCAGCAT+AGG 0.478027 1_4:+12199063 Msa0150570:three_prime_UTR
AATTATAAGCTTGCCAGTGA+TGG 0.484242 1_4:-12198980 Msa0150580:CDS
CAATCTATGGTTTCAGCATA+GGG 0.484769 1_4:+12199064 Msa0150570:three_prime_UTR
GATCTCATGCTCCTAGGTAT+GGG 0.491824 1_4:-12198438 Msa0150580:CDS
CCTATGCTGAAACCATAGAT+TGG 0.494060 1_4:-12199063 Msa0150580:CDS
TGTCATGTTGCCTGTTGAAA+AGG 0.494449 1_4:-12198199 Msa0150580:CDS
AAATAGAGGCATCACTTAAG+TGG 0.496228 1_4:-12199492 Msa0150580:CDS
CGCAGTTTACACTGCACTTG+AGG 0.500062 1_4:-12198235 Msa0150580:CDS
GCATCACTTAAGTGGGAAAA+GGG 0.504570 1_4:-12199484 Msa0150580:CDS
TCTCCAAGAGGATCAGAAAT+GGG 0.506655 1_4:-12198373 Msa0150580:CDS
CAGCTGCTCCATCAGGTGGT+AGG 0.508616 1_4:+12198538 Msa0150570:three_prime_UTR
AAATTTGATCTCATGCTCCT+AGG 0.509138 1_4:-12198444 Msa0150580:CDS
AAAAGGTTTCACCTCTTGAA+GGG 0.515655 1_4:-12198182 Msa0150580:CDS
AGTCACCACCATTGCAACAT+TGG 0.519798 1_4:+12198275 Msa0150570:three_prime_UTR
AGCTGCTCCATCAGGTGGTA+GGG 0.529718 1_4:+12198539 Msa0150570:three_prime_UTR
GTGAAAGTAATTCTCTCTGA+TGG 0.540458 1_4:+12198321 Msa0150570:three_prime_UTR
TTGGAAGAAGCATTGATCAC+TGG 0.541052 1_4:+12198294 Msa0150570:three_prime_UTR
GATCATTAATCAAGTACCTA+AGG 0.543945 1_4:-12199339 Msa0150580:intron
ATTAGTCAAAGTTGTTGAGA+AGG 0.549232 1_4:+12199666 Msa0150570:three_prime_UTR
GCAATGGTGGTGACTGGCGC+GGG 0.551673 1_4:-12198267 Msa0150580:CDS
CTCCTAGGTATGGGTCCAGA+TGG 0.553952 1_4:-12198429 Msa0150580:CDS
AATAGAGGCATCACTTAAGT+GGG 0.554935 1_4:-12199491 Msa0150580:CDS
TTGAAATGGTACTTAGACAA+AGG 0.555841 1_4:-12198156 Msa0150580:CDS
TGCTTCTTCCAATGTTGCAA+TGG 0.565044 1_4:-12198283 Msa0150580:CDS
CATTTCAATTCCCCTTCAAG+AGG 0.566687 1_4:+12198171 Msa0150570:three_prime_UTR
GATGATTCCCTACCACCTGA+TGG 0.566852 1_4:-12198546 Msa0150580:CDS
AATGTTGCAATGGTGGTGAC+TGG 0.569664 1_4:-12198273 Msa0150580:CDS
AGGTGGTAGGGAATCATCAA+TGG 0.569674 1_4:+12198551 Msa0150570:three_prime_UTR
TTCTTCCAATGTTGCAATGG+TGG 0.573190 1_4:-12198280 Msa0150580:CDS
AAAGGGTTATAAGAATGTTG+AGG 0.580239 1_4:-12199467 Msa0150580:CDS
TGCAATGGTGGTGACTGGCG+CGG 0.584998 1_4:-12198268 Msa0150580:CDS
GCCTTAGAGGTTGAAAACCA+AGG 0.590574 1_4:+12199551 Msa0150570:three_prime_UTR
AAAGGTTTCACCTCTTGAAG+GGG 0.598919 1_4:-12198181 Msa0150580:CDS
CTTGGAGAGAAATCCATCAC+TGG 0.601315 1_4:+12198967 Msa0150570:three_prime_UTR
CTTGGAGAAGAGCATGACCT+GGG 0.611662 1_4:+12198388 Msa0150570:three_prime_UTR
AAAGTAATTCTCTCTGATGG+TGG 0.620404 1_4:+12198324 Msa0150570:three_prime_UTR
CAGTGATGGATTTCTCTCCA+AGG 0.622114 1_4:-12198966 Msa0150580:intron
TCTTGGAGAAGAGCATGACC+TGG 0.622800 1_4:+12198387 Msa0150570:three_prime_UTR
TCAATGGCATAAACGTTGAG+AGG 0.637656 1_4:+12198567 Msa0150570:three_prime_UTR
TGATGAGAGAGTTGTCCCAA+AGG 0.638984 1_4:-12199017 Msa0150580:CDS
ATGGCATAAACGTTGAGAGG+AGG 0.640971 1_4:+12198570 Msa0150570:three_prime_UTR
ACATCAGCTGCTCCATCAGG+TGG 0.642046 1_4:+12198534 Msa0150570:three_prime_UTR
AGGTCATGCTCTTCTCCAAG+AGG 0.654284 1_4:-12198385 Msa0150580:CDS
GTCCATCTGGACCCATACCT+AGG 0.668407 1_4:+12198427 Msa0150570:three_prime_UTR
CTCAAGTGCAGTGTAAACTG+CGG 0.695035 1_4:+12198236 Msa0150570:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTAAATGAAATTGTAATT+AGG - chr1_4:12198584-12198603 Msa0150580:intron 10.0%
!! ATACTAGAAATAAATAATTA+AGG + chr1_4:12198655-12198674 Msa0150570:intron 10.0%
!! ATAGTAACATTTATATTAAA+AGG + chr1_4:12198939-12198958 Msa0150570:intron 10.0%
!! ATTTAAATGAAATTGTAATT+AGG - chr1_4:12198584-12198603 Msa0150580:intron 10.0%
!! ATACTAGAAATAAATAATTA+AGG + chr1_4:12198655-12198674 Msa0150570:intron 10.0%
!! ATAGTAACATTTATATTAAA+AGG + chr1_4:12198939-12198958 Msa0150570:intron 10.0%
!! AAATCACATGAATTTAAATT+AGG + chr1_4:12198542-12198561 Msa0150570:three_prime_UTR 15.0%
!! AAATCACATGAATTTAAATT+AGG + chr1_4:12198542-12198561 Msa0150570:three_prime_UTR 15.0%
!!! ATGTGATTTTAATGTTACAT+TGG - chr1_4:12198553-12198572 Msa0150580:CDS 20.0%
!!! AATTAGGATTTTGATGAAAT+TGG - chr1_4:12198600-12198619 Msa0150580:intron 20.0%
!!! ATTTTGATGAAATTGGAATT+AGG - chr1_4:12198607-12198626 Msa0150580:intron 20.0%
!! AGGATTTCAAAAACAAAAAA+TGG - chr1_4:12198627-12198646 Msa0150580:intron 20.0%
!! GAAAATTATGTTTGAATTTC+AGG - chr1_4:12198696-12198715 Msa0150580:intron 20.0%
!!! GAAATTAATCATTTTGATGT+TGG - chr1_4:12198912-12198931 Msa0150580:intron 20.0%
!! ATGATATCGTAATTAGATAA+GGG + chr1_4:12199075-12199094 Msa0150570:three_prime_UTR 20.0%
!! TATGATATCGTAATTAGATA+AGG + chr1_4:12199076-12199095 Msa0150570:three_prime_UTR 20.0%
!! TTATCTAATTACGATATCAT+AGG - chr1_4:12199075-12199094 Msa0150580:CDS 20.0%
!!! ATCAAATTTTGGTAATTCAT+TGG + chr1_4:12199343-12199362 Msa0150570:three_prime_UTR 20.0%
!!! ATGTGATTTTAATGTTACAT+TGG - chr1_4:12198553-12198572 Msa0150580:CDS 20.0%
!!! AATTAGGATTTTGATGAAAT+TGG - chr1_4:12198600-12198619 Msa0150580:intron 20.0%
!!! ATTTTGATGAAATTGGAATT+AGG - chr1_4:12198607-12198626 Msa0150580:intron 20.0%
!! AGGATTTCAAAAACAAAAAA+TGG - chr1_4:12198627-12198646 Msa0150580:intron 20.0%
!! GAAAATTATGTTTGAATTTC+AGG - chr1_4:12198696-12198715 Msa0150580:intron 20.0%
!!! GAAATTAATCATTTTGATGT+TGG - chr1_4:12198912-12198931 Msa0150580:intron 20.0%
!! ATGATATCGTAATTAGATAA+GGG + chr1_4:12199075-12199094 Msa0150570:three_prime_UTR 20.0%
!! TATGATATCGTAATTAGATA+AGG + chr1_4:12199076-12199095 Msa0150570:three_prime_UTR 20.0%
!! TTATCTAATTACGATATCAT+AGG - chr1_4:12199075-12199094 Msa0150580:CDS 20.0%
!!! ATCAAATTTTGGTAATTCAT+TGG + chr1_4:12199343-12199362 Msa0150570:three_prime_UTR 20.0%
! AACAAATTCATCAAAGAAAG+AGG - chr1_4:12198410-12198429 Msa0150580:CDS 25.0%
!!! GATTTTAATGTTACATTGGT+TGG - chr1_4:12198557-12198576 Msa0150580:CDS 25.0%
! ATGTTTGAATTTCAGGAAAT+TGG - chr1_4:12198703-12198722 Msa0150580:intron 25.0%
! AAATCGAACAACTAATCAAA+TGG + chr1_4:12199033-12199052 Msa0150570:three_prime_UTR 25.0%
! GAATTAACAATCATCAAACT+TGG + chr1_4:12199114-12199133 Msa0150570:intron 25.0%
! TCGACAATAAGATTTATTGA+CGG + chr1_4:12199137-12199156 Msa0150570:intron 25.0%
! AACAAATTCATCAAAGAAAG+AGG - chr1_4:12198410-12198429 Msa0150580:CDS 25.0%
!!! GATTTTAATGTTACATTGGT+TGG - chr1_4:12198557-12198576 Msa0150580:CDS 25.0%
! ATGTTTGAATTTCAGGAAAT+TGG - chr1_4:12198703-12198722 Msa0150580:intron 25.0%
! AAATCGAACAACTAATCAAA+TGG + chr1_4:12199033-12199052 Msa0150570:three_prime_UTR 25.0%
! GAATTAACAATCATCAAACT+TGG + chr1_4:12199114-12199133 Msa0150570:intron 25.0%
! TCGACAATAAGATTTATTGA+CGG + chr1_4:12199137-12199156 Msa0150570:intron 25.0%
ATTAGTCAAAGTTGTTGAGA+AGG + chr1_4:12198135-12198154 Msa0150570:three_prime_UTR 30.0%
ATTGATGTCTTTAGTGATAG+TGG + chr1_4:12198203-12198222 Msa0150570:three_prime_UTR 30.0%
! TCAATTTTACCAACCCAAAT+TGG - chr1_4:12198218-12198237 Msa0150580:CDS 30.0%
! CAATTTTACCAACCCAAATT+GGG - chr1_4:12198219-12198238 Msa0150580:CDS 30.0%
AACACTTTATTATGCCTTAG+AGG + chr1_4:12198263-12198282 Msa0150570:three_prime_UTR 30.0%
! TTTTCACCAAATAGATGTGT+CGG - chr1_4:12198281-12198300 Msa0150580:CDS 30.0%
! ATTTTTCCGACACATCTATT+TGG + chr1_4:12198290-12198309 Msa0150570:three_prime_UTR 30.0%
AAAGGGTTATAAGAATGTTG+AGG - chr1_4:12198331-12198350 Msa0150580:CDS 30.0%
GATCATTAATCAAGTACCTA+AGG - chr1_4:12198459-12198478 Msa0150580:CDS 30.0%
TGTTAAGAATTACACACCTT+AGG + chr1_4:12198478-12198497 Msa0150570:three_prime_UTR 30.0%
AAACCATAGATTGGACAAAA+TGG - chr1_4:12198744-12198763 Msa0150580:intron 30.0%
TACTTTCTAAGTGATCCTTT+GGG + chr1_4:12198799-12198818 Msa0150570:intron 30.0%
TTACTTTCTAAGTGATCCTT+TGG + chr1_4:12198800-12198819 Msa0150570:intron 30.0%
TTCAAGCAAAACAATCAACT+TGG + chr1_4:12198873-12198892 Msa0150570:intron 30.0%
CGACAATAAGATTTATTGAC+GGG + chr1_4:12199136-12199155 Msa0150570:intron 30.0%
ATTTCCATTATTCCTTTGTC+AGG - chr1_4:12199197-12199216 Msa0150580:intron 30.0%
TTGAAATGGTACTTAGACAA+AGG - chr1_4:12199642-12199661 Msa0150580:CDS 30.0%
ATTAGTCAAAGTTGTTGAGA+AGG + chr1_4:12198135-12198154 Msa0150570:three_prime_UTR 30.0%
ATTGATGTCTTTAGTGATAG+TGG + chr1_4:12198203-12198222 Msa0150570:three_prime_UTR 30.0%
! TCAATTTTACCAACCCAAAT+TGG - chr1_4:12198218-12198237 Msa0150580:CDS 30.0%
! CAATTTTACCAACCCAAATT+GGG - chr1_4:12198219-12198238 Msa0150580:CDS 30.0%
AACACTTTATTATGCCTTAG+AGG + chr1_4:12198263-12198282 Msa0150570:three_prime_UTR 30.0%
! TTTTCACCAAATAGATGTGT+CGG - chr1_4:12198281-12198300 Msa0150580:CDS 30.0%
! ATTTTTCCGACACATCTATT+TGG + chr1_4:12198290-12198309 Msa0150570:three_prime_UTR 30.0%
AAAGGGTTATAAGAATGTTG+AGG - chr1_4:12198331-12198350 Msa0150580:CDS 30.0%
GATCATTAATCAAGTACCTA+AGG - chr1_4:12198459-12198478 Msa0150580:CDS 30.0%
TGTTAAGAATTACACACCTT+AGG + chr1_4:12198478-12198497 Msa0150570:three_prime_UTR 30.0%
AAACCATAGATTGGACAAAA+TGG - chr1_4:12198744-12198763 Msa0150580:intron 30.0%
TACTTTCTAAGTGATCCTTT+GGG + chr1_4:12198799-12198818 Msa0150570:intron 30.0%
TTACTTTCTAAGTGATCCTT+TGG + chr1_4:12198800-12198819 Msa0150570:intron 30.0%
TTCAAGCAAAACAATCAACT+TGG + chr1_4:12198873-12198892 Msa0150570:intron 30.0%
CGACAATAAGATTTATTGAC+GGG + chr1_4:12199136-12199155 Msa0150570:intron 30.0%
ATTTCCATTATTCCTTTGTC+AGG - chr1_4:12199197-12199216 Msa0150580:intron 30.0%
TTGAAATGGTACTTAGACAA+AGG - chr1_4:12199642-12199661 Msa0150580:CDS 30.0%
GTGCACAAAATAAGCTACTT+TGG + chr1_4:12198169-12198188 Msa0150570:three_prime_UTR 35.0%
! ACCAAGGTTTTTCCCAATTT+GGG + chr1_4:12198234-12198253 Msa0150570:three_prime_UTR 35.0%
! AACCAAGGTTTTTCCCAATT+TGG + chr1_4:12198235-12198254 Msa0150570:three_prime_UTR 35.0%
TAGATGTGTCGGAAAAATAG+AGG - chr1_4:12198292-12198311 Msa0150580:CDS 35.0%
!! AAATAGAGGCATCACTTAAG+TGG - chr1_4:12198306-12198325 Msa0150580:CDS 35.0%
!! AATAGAGGCATCACTTAAGT+GGG - chr1_4:12198307-12198326 Msa0150580:CDS 35.0%
! GAATGTTGAGGTTTTCAGTA+AGG - chr1_4:12198343-12198362 Msa0150580:CDS 35.0%
!! TATTTCACTGTTGCTTTGTC+TGG - chr1_4:12198434-12198453 Msa0150580:CDS 35.0%
CAATCTATGGTTTCAGCATA+GGG + chr1_4:12198737-12198756 Msa0150570:intron 35.0%
!!! ATGCCATTTTGTCCAATCTA+TGG + chr1_4:12198750-12198769 Msa0150570:intron 35.0%
! GACAAAATGGCATGTTTTCT+GGG - chr1_4:12198757-12198776 Msa0150580:intron 35.0%
AATTATAAGCTTGCCAGTGA+TGG - chr1_4:12198818-12198837 Msa0150580:intron 35.0%
GGCACATGAATTAATTACCT+TGG + chr1_4:12198852-12198871 Msa0150570:intron 35.0%
TGCTTGAAGAAGAATGATGA+TGG - chr1_4:12198885-12198904 Msa0150580:intron 35.0%
CTTATTGTCGATCTTTAGTG+AGG - chr1_4:12199146-12199165 Msa0150580:intron 35.0%
! GCTTTTAACCAAACGTCTTA+GGG + chr1_4:12199301-12199320 Msa0150570:intron 35.0%
! TGCTTTTAACCAAACGTCTT+AGG + chr1_4:12199302-12199321 Msa0150570:intron 35.0%
!! AGGAGCATGAGATCAAATTT+TGG + chr1_4:12199354-12199373 Msa0150570:three_prime_UTR 35.0%
AAATTTGATCTCATGCTCCT+AGG - chr1_4:12199354-12199373 Msa0150580:CDS 35.0%
AAAGTAATTCTCTCTGATGG+TGG + chr1_4:12199477-12199496 Msa0150570:three_prime_UTR 35.0%
GTGAAAGTAATTCTCTCTGA+TGG + chr1_4:12199480-12199499 Msa0150570:three_prime_UTR 35.0%
AAAAGGTTTCACCTCTTGAA+GGG - chr1_4:12199616-12199635 Msa0150580:CDS 35.0%
! TGCTGCTTCAAAACTGTTTA+AGG - chr1_4:12199665-12199684 Msa0150580:CDS 35.0%
GTGCACAAAATAAGCTACTT+TGG + chr1_4:12198169-12198188 Msa0150570:three_prime_UTR 35.0%
! ACCAAGGTTTTTCCCAATTT+GGG + chr1_4:12198234-12198253 Msa0150570:three_prime_UTR 35.0%
! AACCAAGGTTTTTCCCAATT+TGG + chr1_4:12198235-12198254 Msa0150570:three_prime_UTR 35.0%
TAGATGTGTCGGAAAAATAG+AGG - chr1_4:12198292-12198311 Msa0150580:CDS 35.0%
!! AAATAGAGGCATCACTTAAG+TGG - chr1_4:12198306-12198325 Msa0150580:CDS 35.0%
!! AATAGAGGCATCACTTAAGT+GGG - chr1_4:12198307-12198326 Msa0150580:CDS 35.0%
! GAATGTTGAGGTTTTCAGTA+AGG - chr1_4:12198343-12198362 Msa0150580:CDS 35.0%
!! TATTTCACTGTTGCTTTGTC+TGG - chr1_4:12198434-12198453 Msa0150580:CDS 35.0%
CAATCTATGGTTTCAGCATA+GGG + chr1_4:12198737-12198756 Msa0150570:intron 35.0%
!!! ATGCCATTTTGTCCAATCTA+TGG + chr1_4:12198750-12198769 Msa0150570:intron 35.0%
! GACAAAATGGCATGTTTTCT+GGG - chr1_4:12198757-12198776 Msa0150580:intron 35.0%
AATTATAAGCTTGCCAGTGA+TGG - chr1_4:12198818-12198837 Msa0150580:intron 35.0%
GGCACATGAATTAATTACCT+TGG + chr1_4:12198852-12198871 Msa0150570:intron 35.0%
TGCTTGAAGAAGAATGATGA+TGG - chr1_4:12198885-12198904 Msa0150580:intron 35.0%
CTTATTGTCGATCTTTAGTG+AGG - chr1_4:12199146-12199165 Msa0150580:intron 35.0%
! GCTTTTAACCAAACGTCTTA+GGG + chr1_4:12199301-12199320 Msa0150570:intron 35.0%
! TGCTTTTAACCAAACGTCTT+AGG + chr1_4:12199302-12199321 Msa0150570:intron 35.0%
!! AGGAGCATGAGATCAAATTT+TGG + chr1_4:12199354-12199373 Msa0150570:three_prime_UTR 35.0%
AAATTTGATCTCATGCTCCT+AGG - chr1_4:12199354-12199373 Msa0150580:CDS 35.0%
AAAGTAATTCTCTCTGATGG+TGG + chr1_4:12199477-12199496 Msa0150570:three_prime_UTR 35.0%
GTGAAAGTAATTCTCTCTGA+TGG + chr1_4:12199480-12199499 Msa0150570:three_prime_UTR 35.0%
AAAAGGTTTCACCTCTTGAA+GGG - chr1_4:12199616-12199635 Msa0150580:CDS 35.0%
! TGCTGCTTCAAAACTGTTTA+AGG - chr1_4:12199665-12199684 Msa0150580:CDS 35.0%
! AGGTTTTTCCCAATTTGGGT+TGG + chr1_4:12198230-12198249 Msa0150570:three_prime_UTR 40.0%
ACCCAAATTGGGAAAAACCT+TGG - chr1_4:12198230-12198249 Msa0150580:CDS 40.0%
!! ACCTTGGTTTTCAACCTCTA+AGG - chr1_4:12198246-12198265 Msa0150580:CDS 40.0%
GCATCACTTAAGTGGGAAAA+GGG - chr1_4:12198314-12198333 Msa0150580:CDS 40.0%
!! GGAACATCTTGCTGTTTCAT+TGG - chr1_4:12198364-12198383 Msa0150580:CDS 40.0%
CCAATCTATGGTTTCAGCAT+AGG + chr1_4:12198738-12198757 Msa0150570:intron 40.0%
CCTATGCTGAAACCATAGAT+TGG - chr1_4:12198735-12198754 Msa0150580:intron 40.0%
! GGACAAAATGGCATGTTTTC+TGG - chr1_4:12198756-12198775 Msa0150580:intron 40.0%
TACTAGCATATCGTGACATG+TGG - chr1_4:12198987-12199006 Msa0150580:CDS 40.0%
ATTACGATATCATAGGTGCG+AGG - chr1_4:12199082-12199101 Msa0150580:CDS 40.0%
TAAACGTTGAGAGGAGGAAT+TGG + chr1_4:12199225-12199244 Msa0150570:intron 40.0%
TCAATGGCATAAACGTTGAG+AGG + chr1_4:12199234-12199253 Msa0150570:intron 40.0%
TGAAACAACCCTAAGACGTT+TGG - chr1_4:12199290-12199309 Msa0150580:intron 40.0%
AAAGATGCAACATGTCCATC+TGG + chr1_4:12199387-12199406 Msa0150570:three_prime_UTR 40.0%
CATGTTGCATCTTTATTCCC+AGG - chr1_4:12199393-12199412 Msa0150580:CDS 40.0%
TTCTCCAAGAGGATCAGAAA+TGG - chr1_4:12199424-12199443 Msa0150580:CDS 40.0%
TCTCCAAGAGGATCAGAAAT+GGG - chr1_4:12199425-12199444 Msa0150580:CDS 40.0%
TAACCCATTTCTGATCCTCT+TGG + chr1_4:12199431-12199450 Msa0150570:three_prime_UTR 40.0%
!!! ATTCTCTCTGATGGTGGTTT+TGG + chr1_4:12199471-12199490 Msa0150570:three_prime_UTR 40.0%
!! TTGGAAGAAGCATTGATCAC+TGG + chr1_4:12199507-12199526 Msa0150570:three_prime_UTR 40.0%
TGCTTCTTCCAATGTTGCAA+TGG - chr1_4:12199515-12199534 Msa0150580:CDS 40.0%
TTCTTCCAATGTTGCAATGG+TGG - chr1_4:12199518-12199537 Msa0150580:CDS 40.0%
TGTCATGTTGCCTGTTGAAA+AGG - chr1_4:12199599-12199618 Msa0150580:CDS 40.0%
! AGAGGTGAAACCTTTTCAAC+AGG + chr1_4:12199612-12199631 Msa0150570:three_prime_UTR 40.0%
GAAAAGGTTTCACCTCTTGA+AGG - chr1_4:12199615-12199634 Msa0150580:CDS 40.0%
AAAGGTTTCACCTCTTGAAG+GGG - chr1_4:12199617-12199636 Msa0150580:CDS 40.0%
CATTTCAATTCCCCTTCAAG+AGG + chr1_4:12199630-12199649 Msa0150570:three_prime_UTR 40.0%
CTCTTGAAGGGGAATTGAAA+TGG - chr1_4:12199628-12199647 Msa0150580:CDS 40.0%
! AGGTTTTTCCCAATTTGGGT+TGG + chr1_4:12198230-12198249 Msa0150570:three_prime_UTR 40.0%
ACCCAAATTGGGAAAAACCT+TGG - chr1_4:12198230-12198249 Msa0150580:CDS 40.0%
!! ACCTTGGTTTTCAACCTCTA+AGG - chr1_4:12198246-12198265 Msa0150580:CDS 40.0%
GCATCACTTAAGTGGGAAAA+GGG - chr1_4:12198314-12198333 Msa0150580:CDS 40.0%
!! GGAACATCTTGCTGTTTCAT+TGG - chr1_4:12198364-12198383 Msa0150580:CDS 40.0%
CCAATCTATGGTTTCAGCAT+AGG + chr1_4:12198738-12198757 Msa0150570:intron 40.0%
CCTATGCTGAAACCATAGAT+TGG - chr1_4:12198735-12198754 Msa0150580:intron 40.0%
! GGACAAAATGGCATGTTTTC+TGG - chr1_4:12198756-12198775 Msa0150580:intron 40.0%
TACTAGCATATCGTGACATG+TGG - chr1_4:12198987-12199006 Msa0150580:CDS 40.0%
ATTACGATATCATAGGTGCG+AGG - chr1_4:12199082-12199101 Msa0150580:CDS 40.0%
TAAACGTTGAGAGGAGGAAT+TGG + chr1_4:12199225-12199244 Msa0150570:intron 40.0%
TCAATGGCATAAACGTTGAG+AGG + chr1_4:12199234-12199253 Msa0150570:intron 40.0%
TGAAACAACCCTAAGACGTT+TGG - chr1_4:12199290-12199309 Msa0150580:intron 40.0%
AAAGATGCAACATGTCCATC+TGG + chr1_4:12199387-12199406 Msa0150570:three_prime_UTR 40.0%
CATGTTGCATCTTTATTCCC+AGG - chr1_4:12199393-12199412 Msa0150580:CDS 40.0%
TTCTCCAAGAGGATCAGAAA+TGG - chr1_4:12199424-12199443 Msa0150580:CDS 40.0%
TCTCCAAGAGGATCAGAAAT+GGG - chr1_4:12199425-12199444 Msa0150580:CDS 40.0%
TAACCCATTTCTGATCCTCT+TGG + chr1_4:12199431-12199450 Msa0150570:three_prime_UTR 40.0%
!!! ATTCTCTCTGATGGTGGTTT+TGG + chr1_4:12199471-12199490 Msa0150570:three_prime_UTR 40.0%
!! TTGGAAGAAGCATTGATCAC+TGG + chr1_4:12199507-12199526 Msa0150570:three_prime_UTR 40.0%
TGCTTCTTCCAATGTTGCAA+TGG - chr1_4:12199515-12199534 Msa0150580:CDS 40.0%
TTCTTCCAATGTTGCAATGG+TGG - chr1_4:12199518-12199537 Msa0150580:CDS 40.0%
TGTCATGTTGCCTGTTGAAA+AGG - chr1_4:12199599-12199618 Msa0150580:CDS 40.0%
! AGAGGTGAAACCTTTTCAAC+AGG + chr1_4:12199612-12199631 Msa0150570:three_prime_UTR 40.0%
GAAAAGGTTTCACCTCTTGA+AGG - chr1_4:12199615-12199634 Msa0150580:CDS 40.0%
AAAGGTTTCACCTCTTGAAG+GGG - chr1_4:12199617-12199636 Msa0150580:CDS 40.0%
CATTTCAATTCCCCTTCAAG+AGG + chr1_4:12199630-12199649 Msa0150570:three_prime_UTR 40.0%
CTCTTGAAGGGGAATTGAAA+TGG - chr1_4:12199628-12199647 Msa0150580:CDS 40.0%
GCCTTAGAGGTTGAAAACCA+AGG + chr1_4:12198250-12198269 Msa0150570:three_prime_UTR 45.0%
GGCATCACTTAAGTGGGAAA+AGG - chr1_4:12198313-12198332 Msa0150580:CDS 45.0%
!! TTCACTGTTGCTTTGTCTGG+TGG - chr1_4:12198437-12198456 Msa0150580:CDS 45.0%
TGATGAGAGAGTTGTCCCAA+AGG - chr1_4:12198781-12198800 Msa0150580:intron 45.0%
CTTGGAGAGAAATCCATCAC+TGG + chr1_4:12198834-12198853 Msa0150570:intron 45.0%
!! CAGTGATGGATTTCTCTCCA+AGG - chr1_4:12198832-12198851 Msa0150580:intron 45.0%
GGCACCTGACAAAGGAATAA+TGG + chr1_4:12199204-12199223 Msa0150570:intron 45.0%
ATGGCATAAACGTTGAGAGG+AGG + chr1_4:12199231-12199250 Msa0150570:intron 45.0%
AGGTGGTAGGGAATCATCAA+TGG + chr1_4:12199250-12199269 Msa0150570:intron 45.0%
TAAACATCAGCTGCTCCATC+AGG + chr1_4:12199270-12199289 Msa0150570:intron 45.0%
TGATCTCATGCTCCTAGGTA+TGG - chr1_4:12199359-12199378 Msa0150580:CDS 45.0%
GATCTCATGCTCCTAGGTAT+GGG - chr1_4:12199360-12199379 Msa0150580:CDS 45.0%
AGTCACCACCATTGCAACAT+TGG + chr1_4:12199526-12199545 Msa0150570:three_prime_UTR 45.0%
! AATGTTGCAATGGTGGTGAC+TGG - chr1_4:12199525-12199544 Msa0150580:CDS 45.0%
CTCAAGTGCAGTGTAAACTG+CGG + chr1_4:12199565-12199584 Msa0150570:three_prime_UTR 45.0%
GCCTTAGAGGTTGAAAACCA+AGG + chr1_4:12198250-12198269 Msa0150570:three_prime_UTR 45.0%
GGCATCACTTAAGTGGGAAA+AGG - chr1_4:12198313-12198332 Msa0150580:CDS 45.0%
!! TTCACTGTTGCTTTGTCTGG+TGG - chr1_4:12198437-12198456 Msa0150580:CDS 45.0%
TGATGAGAGAGTTGTCCCAA+AGG - chr1_4:12198781-12198800 Msa0150580:intron 45.0%
CTTGGAGAGAAATCCATCAC+TGG + chr1_4:12198834-12198853 Msa0150570:intron 45.0%
!! CAGTGATGGATTTCTCTCCA+AGG - chr1_4:12198832-12198851 Msa0150580:intron 45.0%
GGCACCTGACAAAGGAATAA+TGG + chr1_4:12199204-12199223 Msa0150570:intron 45.0%
ATGGCATAAACGTTGAGAGG+AGG + chr1_4:12199231-12199250 Msa0150570:intron 45.0%
AGGTGGTAGGGAATCATCAA+TGG + chr1_4:12199250-12199269 Msa0150570:intron 45.0%
TAAACATCAGCTGCTCCATC+AGG + chr1_4:12199270-12199289 Msa0150570:intron 45.0%
TGATCTCATGCTCCTAGGTA+TGG - chr1_4:12199359-12199378 Msa0150580:CDS 45.0%
GATCTCATGCTCCTAGGTAT+GGG - chr1_4:12199360-12199379 Msa0150580:CDS 45.0%
AGTCACCACCATTGCAACAT+TGG + chr1_4:12199526-12199545 Msa0150570:three_prime_UTR 45.0%
! AATGTTGCAATGGTGGTGAC+TGG - chr1_4:12199525-12199544 Msa0150580:CDS 45.0%
CTCAAGTGCAGTGTAAACTG+CGG + chr1_4:12199565-12199584 Msa0150570:three_prime_UTR 45.0%
GAGGAATTGGCACCTGACAA+AGG + chr1_4:12199212-12199231 Msa0150570:intron 50.0%
GATGATTCCCTACCACCTGA+TGG - chr1_4:12199252-12199271 Msa0150580:intron 50.0%
! CTTGGAGAAGAGCATGACCT+GGG + chr1_4:12199413-12199432 Msa0150570:three_prime_UTR 50.0%
! TCTTGGAGAAGAGCATGACC+TGG + chr1_4:12199414-12199433 Msa0150570:three_prime_UTR 50.0%
AGGTCATGCTCTTCTCCAAG+AGG - chr1_4:12199413-12199432 Msa0150580:CDS 50.0%
!!! GTGGTTTTGGTGAGTCAGTG+AGG + chr1_4:12199458-12199477 Msa0150570:three_prime_UTR 50.0%
CGCAGTTTACACTGCACTTG+AGG - chr1_4:12199563-12199582 Msa0150580:CDS 50.0%
GAGGAATTGGCACCTGACAA+AGG + chr1_4:12199212-12199231 Msa0150570:intron 50.0%
GATGATTCCCTACCACCTGA+TGG - chr1_4:12199252-12199271 Msa0150580:intron 50.0%
! CTTGGAGAAGAGCATGACCT+GGG + chr1_4:12199413-12199432 Msa0150570:three_prime_UTR 50.0%
! TCTTGGAGAAGAGCATGACC+TGG + chr1_4:12199414-12199433 Msa0150570:three_prime_UTR 50.0%
AGGTCATGCTCTTCTCCAAG+AGG - chr1_4:12199413-12199432 Msa0150580:CDS 50.0%
!!! GTGGTTTTGGTGAGTCAGTG+AGG + chr1_4:12199458-12199477 Msa0150570:three_prime_UTR 50.0%
CGCAGTTTACACTGCACTTG+AGG - chr1_4:12199563-12199582 Msa0150580:CDS 50.0%
AGCTGCTCCATCAGGTGGTA+GGG + chr1_4:12199262-12199281 Msa0150570:intron 55.0%
ACATCAGCTGCTCCATCAGG+TGG + chr1_4:12199267-12199286 Msa0150570:intron 55.0%
CTCCTAGGTATGGGTCCAGA+TGG - chr1_4:12199369-12199388 Msa0150580:CDS 55.0%
GTCCATCTGGACCCATACCT+AGG + chr1_4:12199374-12199393 Msa0150570:three_prime_UTR 55.0%
AGCTGCTCCATCAGGTGGTA+GGG + chr1_4:12199262-12199281 Msa0150570:intron 55.0%
ACATCAGCTGCTCCATCAGG+TGG + chr1_4:12199267-12199286 Msa0150570:intron 55.0%
CTCCTAGGTATGGGTCCAGA+TGG - chr1_4:12199369-12199388 Msa0150580:CDS 55.0%
GTCCATCTGGACCCATACCT+AGG + chr1_4:12199374-12199393 Msa0150570:three_prime_UTR 55.0%
CAGCTGCTCCATCAGGTGGT+AGG + chr1_4:12199263-12199282 Msa0150570:intron 60.0%
! TGCAATGGTGGTGACTGGCG+CGG - chr1_4:12199530-12199549 Msa0150580:CDS 60.0%
CAGCTGCTCCATCAGGTGGT+AGG + chr1_4:12199263-12199282 Msa0150570:intron 60.0%
! TGCAATGGTGGTGACTGGCG+CGG - chr1_4:12199530-12199549 Msa0150580:CDS 60.0%
! GCAATGGTGGTGACTGGCGC+GGG - chr1_4:12199531-12199550 Msa0150580:CDS 65.0%
! GCAATGGTGGTGACTGGCGC+GGG - chr1_4:12199531-12199550 Msa0150580:CDS 65.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 12198128 12199692 12198128 ID=Msa0150580;Name=Msa0150580
chr1_4 mRNA 12198128 12199692 12198128 ID=Msa0150580-mRNA-1;Parent=Msa0150580;Name=Msa0150580-mRNA-1;_AED=0.32;_eAED=0.32;_QI=0|0|0|1|1|1|3|0|320
chr1_4 exon 12199340 12199692 12199340 ID=Msa0150580-mRNA-1:exon:4987;Parent=Msa0150580-mRNA-1
chr1_4 exon 12198967 12199102 12198967 ID=Msa0150580-mRNA-1:exon:4986;Parent=Msa0150580-mRNA-1
chr1_4 exon 12198128 12198601 12198128 ID=Msa0150580-mRNA-1:exon:4985;Parent=Msa0150580-mRNA-1
chr1_4 CDS 12199340 12199692 12199340 ID=Msa0150580-mRNA-1:cds;Parent=Msa0150580-mRNA-1
chr1_4 CDS 12198967 12199102 12198967 ID=Msa0150580-mRNA-1:cds;Parent=Msa0150580-mRNA-1
chr1_4 CDS 12198128 12198601 12198128 ID=Msa0150580-mRNA-1:cds;Parent=Msa0150580-mRNA-1
Gene Sequence

>Msa0150580

ATGGCCTTCTCAACAACTTTGACTAATTCATGCACTCTCCAAAGTAGCTTATTTTGTGCACAAAAATCAAATCCACTATCACTAAAGACATCAATTTTACCAACCCAAATTGGGAAAAACCTTGGTTTTCAACCTCTAAGGCATAATAAAGTGTTTTCACCAAATAGATGTGTCGGAAAAATAGAGGCATCACTTAAGTGGGAAAAGGGTTATAAGAATGTTGAGGTTTTCAGTAAGGAACATCTTGCTGTTTCATTGGCTTATGATGTTGCTCAACTTTCTAACAAATTCATCAAAGAAAGAGGTTATTTCACTGTTGCTTTGTCTGGTGGATCATTAATCAAGTACCTAAGGAAATTGGTTGAATCTCCCTATGCTGAAACCATAGATTGGACAAAATGGCATGTTTTCTGGGTTGATGAGAGAGTTGTCCCAAAGGATCACTTAGAAAGTAATTATAAGCTTGCCAGTGATGGATTTCTCTCCAAGGTGCCAATTCCTCCTCTCAACGTTTATGCCATTGATGATTCCCTACCACCTGATGGAGCAGCTGATGTTTATGAAACAACCCTAAGACGTTTGGTTAAAAGCAATGTGATATCTACATCAACCAATGAATTACCAAAATTTGATCTCATGCTCCTAGGTATGGGTCCAGATGGACATGTTGCATCTTTATTCCCAGGTCATGCTCTTCTCCAAGAGGATCAGAAATGGGTTACTTTCCTCACTGACTCACCAAAACCACCATCAGAGAGAATTACTTTCACTTTTCCAGTGATCAATGCTTCTTCCAATGTTGCAATGGTGGTGACTGGCGCGGGTAAATCGACCGCAGTTTACACTGCACTTGAGGATGAGAAGAAAACTGTCATGTTGCCTGTTGAAAAGGTTTCACCTCTTGAAGGGGAATTGAAATGGTACTTAGACAAAGGTGCTGCTTCAAAACTGTTTAAGGAGTAA
Protein sequence

>Msa0150580

MAFSTTLTNSCTLQSSLFCAQKSNPLSLKTSILPTQIGKNLGFQPLRHNKVFSPNRCVGKIEASLKWEKGYKNVEVFSKEHLAVSLAYDVAQLSNKFIKERGYFTVALSGGSLIKYLRKLVESPYAETIDWTKWHVFWVDERVVPKDHLESNYKLASDGFLSKVPIPPLNVYAIDDSLPPDGAADVYETTLRRLVKSNVISTSTNELPKFDLMLLGMGPDGHVASLFPGHALLQEDQKWVTFLTDSPKPPSERITFTFPVINASSNVAMVVTGAGKSTAVYTALEDEKKTVMLPVEKVSPLEGELKWYLDKGAASKLFKE*