Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0018690 | sp|Q9M1K1|ORG2_ARATH | 43.805 | 226 | 118 | 4 | 1 | 219 | 1 | 224 | 2.95e-53 | 174 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|---|---|
| Msa0018690 | TF | bHLH |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0018690 | Msa0112640 | 0.833364 | 5.563605e-56 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0018690 | MtrunA17_Chr1g0170581 | 94.521 | 219 | 12 | 0 | 1 | 219 | 1 | 219 | 4.89e-151 | 419 |
| Msa0018690 | MtrunA17_Chr1g0170591 | 85.388 | 219 | 24 | 1 | 1 | 219 | 1 | 211 | 4.50e-135 | 379 |
| Msa0018690 | MtrunA17_Chr1g0170571 | 90.868 | 219 | 12 | 1 | 1 | 219 | 1 | 211 | 9.16e-134 | 375 |
| Msa0018690 | MtrunA17_Chr7g0256671 | 57.642 | 229 | 78 | 7 | 1 | 219 | 1 | 220 | 2.86e-58 | 184 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0018690 | AT3G56970.1 | 43.805 | 226 | 118 | 4 | 1 | 219 | 1 | 224 | 3.00e-54 | 174 |
| Msa0018690 | AT2G41240.1 | 39.732 | 224 | 122 | 3 | 1 | 219 | 1 | 216 | 1.44e-43 | 147 |
| Msa0018690 | AT3G56980.1 | 40.726 | 248 | 99 | 5 | 1 | 219 | 1 | 229 | 1.86e-43 | 147 |
| Msa0018690 | AT2G41240.2 | 39.286 | 224 | 122 | 4 | 1 | 219 | 1 | 215 | 4.56e-41 | 140 |
| Msa0018690 | AT5G04150.2 | 45.806 | 155 | 82 | 1 | 65 | 219 | 86 | 238 | 2.31e-36 | 128 |
| Msa0018690 | AT5G04150.1 | 45.161 | 155 | 83 | 1 | 65 | 219 | 86 | 238 | 2.35e-35 | 126 |
Find 38 sgRNAs with CRISPR-Local
Find 106 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAACTTGTTTCTGTAATTC+TGG | 0.201065 | 1_1:-31666148 | None:intergenic |
| TGTAACTATGCTTCTTCCTT+TGG | 0.251062 | 1_1:-31666249 | None:intergenic |
| CTTCTATAATTTGCATTTCC+AGG | 0.265122 | 1_1:+31666441 | Msa0018690:CDS |
| TTCTGAGATCTTGATATGTT+TGG | 0.287728 | 1_1:+31666360 | Msa0018690:CDS |
| GTAACTATGCTTCTTCCTTT+GGG | 0.307422 | 1_1:-31666248 | None:intergenic |
| TCGTTCACTTCTTCCTGTTT+CGG | 0.314619 | 1_1:+31665860 | Msa0018690:CDS |
| CTTTCTTCATCTCAGACCTT+TGG | 0.339976 | 1_1:+31666409 | Msa0018690:CDS |
| TCTTCATCTCAGACCTTTGG+AGG | 0.399010 | 1_1:+31666412 | Msa0018690:CDS |
| AGTACCATTTGATCCGAAAC+AGG | 0.404716 | 1_1:-31665873 | None:intergenic |
| GATCTCGTTCACTAGCATTA+TGG | 0.405770 | 1_1:-31665803 | None:intergenic |
| AGGACTGTTGAAATTGTTGC+TGG | 0.413979 | 1_1:-31666115 | None:intergenic |
| ATGAATTCTGATGATGGTTA+TGG | 0.420513 | 1_1:-31665662 | None:intergenic |
| TCTTCCTGTTTCGGATCAAA+TGG | 0.449037 | 1_1:+31665869 | Msa0018690:CDS |
| AACAATTGGATGGCCCTTTG+AGG | 0.464251 | 1_1:+31665629 | Msa0018690:CDS |
| TGAAGGACTAAGTAAGAGAA+AGG | 0.469978 | 1_1:+31666168 | Msa0018690:CDS |
| CATATCAAGATCTCAGAAAG+TGG | 0.493296 | 1_1:-31666355 | None:intergenic |
| AAGAAAGATTAAGAAGCAAA+AGG | 0.505181 | 1_1:-31666393 | None:intergenic |
| AGAATAACCTCTTCTTCTTG+AGG | 0.516991 | 1_1:-31666214 | None:intergenic |
| CCTAGTGAAATTAACACACC+TGG | 0.521834 | 1_1:-31666459 | None:intergenic |
| TGTAGAATGAATTCTGATGA+TGG | 0.522101 | 1_1:-31665668 | None:intergenic |
| CATGCTAAGGTCACTGCTTG+TGG | 0.529098 | 1_1:-31665766 | None:intergenic |
| ACAAATTATTGATCTTCTTG+CGG | 0.529574 | 1_1:-31665827 | None:intergenic |
| TTGATATGTTTGGAAAATGA+TGG | 0.537710 | 1_1:+31666370 | Msa0018690:CDS |
| ACTTTCTCCTCAAGAAGAAG+AGG | 0.540108 | 1_1:+31666207 | Msa0018690:CDS |
| CTTCATCTCAGACCTTTGGA+GGG | 0.548412 | 1_1:+31666413 | Msa0018690:CDS |
| AAGAAGCATAGTTACAACTC+TGG | 0.551749 | 1_1:+31666256 | Msa0018690:CDS |
| GAATTACAGAAACAAGTTGA+AGG | 0.556198 | 1_1:+31666151 | Msa0018690:CDS |
| CATCCAATTGTTGAAAACAT+AGG | 0.558093 | 1_1:-31665618 | None:intergenic |
| TCTCAGAAAGTGGAATCTTG+TGG | 0.569417 | 1_1:-31666345 | None:intergenic |
| AGTGTAACATGATATCTGAA+TGG | 0.570967 | 1_1:-31666320 | None:intergenic |
| CCAATTGTTGAAAACATAGG+AGG | 0.582074 | 1_1:-31665615 | None:intergenic |
| ATAGAAGACTCTCCCTCCAA+AGG | 0.594272 | 1_1:-31666425 | None:intergenic |
| GTCACTGCTTGTGGATTGTG+AGG | 0.596424 | 1_1:-31665757 | None:intergenic |
| AGGAAGAAGTGAACGAAGTG+AGG | 0.599839 | 1_1:-31665853 | None:intergenic |
| CAAGCTTCTTGACCATGCTA+AGG | 0.604223 | 1_1:-31665779 | None:intergenic |
| CACAAGCAGTGACCTTAGCA+TGG | 0.613188 | 1_1:+31665767 | Msa0018690:CDS |
| TTCTAAGCAAAGAATCCCAA+AGG | 0.620328 | 1_1:+31666233 | Msa0018690:CDS |
| TCACTGCTTGTGGATTGTGA+GGG | 0.698792 | 1_1:-31665756 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAATTTTCATGATCAAGTTG+AGG | + | chr1_1:31665713-31665732 | Msa0018690:CDS | 25.0% |
| ! | ACAAATTATTGATCTTCTTG+CGG | - | chr1_1:31665830-31665849 | None:intergenic | 25.0% |
| ! | ACAATTAGAAACAGAAAGAA+GGG | - | chr1_1:31665907-31665926 | None:intergenic | 25.0% |
| ! | TCTAATTGTCAATAATGCTT+TGG | + | chr1_1:31665918-31665937 | Msa0018690:intron | 25.0% |
| !! | GTAATTCTGGTATGTATTTT+AGG | - | chr1_1:31666138-31666157 | None:intergenic | 25.0% |
| ! | TTGATATGTTTGGAAAATGA+TGG | + | chr1_1:31666370-31666389 | Msa0018690:CDS | 25.0% |
| ! | AAGAAAGATTAAGAAGCAAA+AGG | - | chr1_1:31666396-31666415 | None:intergenic | 25.0% |
| !!! | TAATTTTCATGATCAAGTTG+AGG | + | chr1_1:31665713-31665732 | Msa0018690:CDS | 25.0% |
| ! | ACAAATTATTGATCTTCTTG+CGG | - | chr1_1:31665830-31665849 | None:intergenic | 25.0% |
| ! | ACAATTAGAAACAGAAAGAA+GGG | - | chr1_1:31665907-31665926 | None:intergenic | 25.0% |
| ! | TCTAATTGTCAATAATGCTT+TGG | + | chr1_1:31665918-31665937 | Msa0018690:intron | 25.0% |
| !! | GTAATTCTGGTATGTATTTT+AGG | - | chr1_1:31666138-31666157 | None:intergenic | 25.0% |
| ! | TTGATATGTTTGGAAAATGA+TGG | + | chr1_1:31666370-31666389 | Msa0018690:CDS | 25.0% |
| ! | AAGAAAGATTAAGAAGCAAA+AGG | - | chr1_1:31666396-31666415 | None:intergenic | 25.0% |
| CATCCAATTGTTGAAAACAT+AGG | - | chr1_1:31665621-31665640 | None:intergenic | 30.0% | |
| ! | CTATGTTTTCAACAATTGGA+TGG | + | chr1_1:31665619-31665638 | Msa0018690:CDS | 30.0% |
| !! | ATGAATTCTGATGATGGTTA+TGG | - | chr1_1:31665665-31665684 | None:intergenic | 30.0% |
| TGTAGAATGAATTCTGATGA+TGG | - | chr1_1:31665671-31665690 | None:intergenic | 30.0% | |
| CAATTAGAAACAGAAAGAAG+GGG | - | chr1_1:31665906-31665925 | None:intergenic | 30.0% | |
| GACAATTAGAAACAGAAAGA+AGG | - | chr1_1:31665908-31665927 | None:intergenic | 30.0% | |
| !!! | TCTAATTGTGATGTCGTTTT+TGG | + | chr1_1:31666016-31666035 | Msa0018690:intron | 30.0% |
| !! | TCAACTTGTTTCTGTAATTC+TGG | - | chr1_1:31666151-31666170 | None:intergenic | 30.0% |
| ! | GAATTACAGAAACAAGTTGA+AGG | + | chr1_1:31666151-31666170 | Msa0018690:CDS | 30.0% |
| !! | GTTTTGTAGTTTCAAGTAGT+AGG | + | chr1_1:31666278-31666297 | Msa0018690:CDS | 30.0% |
| AGTGTAACATGATATCTGAA+TGG | - | chr1_1:31666323-31666342 | None:intergenic | 30.0% | |
| !! | TTCTGAGATCTTGATATGTT+TGG | + | chr1_1:31666360-31666379 | Msa0018690:CDS | 30.0% |
| ! | CTTCTATAATTTGCATTTCC+AGG | + | chr1_1:31666441-31666460 | Msa0018690:CDS | 30.0% |
| CATCCAATTGTTGAAAACAT+AGG | - | chr1_1:31665621-31665640 | None:intergenic | 30.0% | |
| ! | CTATGTTTTCAACAATTGGA+TGG | + | chr1_1:31665619-31665638 | Msa0018690:CDS | 30.0% |
| !! | ATGAATTCTGATGATGGTTA+TGG | - | chr1_1:31665665-31665684 | None:intergenic | 30.0% |
| TGTAGAATGAATTCTGATGA+TGG | - | chr1_1:31665671-31665690 | None:intergenic | 30.0% | |
| CAATTAGAAACAGAAAGAAG+GGG | - | chr1_1:31665906-31665925 | None:intergenic | 30.0% | |
| GACAATTAGAAACAGAAAGA+AGG | - | chr1_1:31665908-31665927 | None:intergenic | 30.0% | |
| !!! | TCTAATTGTGATGTCGTTTT+TGG | + | chr1_1:31666016-31666035 | Msa0018690:intron | 30.0% |
| !! | TCAACTTGTTTCTGTAATTC+TGG | - | chr1_1:31666151-31666170 | None:intergenic | 30.0% |
| ! | GAATTACAGAAACAAGTTGA+AGG | + | chr1_1:31666151-31666170 | Msa0018690:CDS | 30.0% |
| !! | GTTTTGTAGTTTCAAGTAGT+AGG | + | chr1_1:31666278-31666297 | Msa0018690:CDS | 30.0% |
| AGTGTAACATGATATCTGAA+TGG | - | chr1_1:31666323-31666342 | None:intergenic | 30.0% | |
| !! | TTCTGAGATCTTGATATGTT+TGG | + | chr1_1:31666360-31666379 | Msa0018690:CDS | 30.0% |
| ! | CTTCTATAATTTGCATTTCC+AGG | + | chr1_1:31666441-31666460 | Msa0018690:CDS | 30.0% |
| CCAATTGTTGAAAACATAGG+AGG | - | chr1_1:31665618-31665637 | None:intergenic | 35.0% | |
| ! | CCTCCTATGTTTTCAACAAT+TGG | + | chr1_1:31665615-31665634 | Msa0018690:CDS | 35.0% |
| !!! | TGATGATGGTTATGGCTTTT+TGG | - | chr1_1:31665657-31665676 | None:intergenic | 35.0% |
| ! | CTTGAAAACCTTGTGTTTTG+AGG | + | chr1_1:31665977-31665996 | Msa0018690:intron | 35.0% |
| TGAAGGACTAAGTAAGAGAA+AGG | + | chr1_1:31666168-31666187 | Msa0018690:CDS | 35.0% | |
| AGAATAACCTCTTCTTCTTG+AGG | - | chr1_1:31666217-31666236 | None:intergenic | 35.0% | |
| TTCTAAGCAAAGAATCCCAA+AGG | + | chr1_1:31666233-31666252 | Msa0018690:CDS | 35.0% | |
| GTAACTATGCTTCTTCCTTT+GGG | - | chr1_1:31666251-31666270 | None:intergenic | 35.0% | |
| TGTAACTATGCTTCTTCCTT+TGG | - | chr1_1:31666252-31666271 | None:intergenic | 35.0% | |
| ! | AAGAAGCATAGTTACAACTC+TGG | + | chr1_1:31666256-31666275 | Msa0018690:CDS | 35.0% |
| CATATCAAGATCTCAGAAAG+TGG | - | chr1_1:31666358-31666377 | None:intergenic | 35.0% | |
| CCAATTGTTGAAAACATAGG+AGG | - | chr1_1:31665618-31665637 | None:intergenic | 35.0% | |
| ! | CCTCCTATGTTTTCAACAAT+TGG | + | chr1_1:31665615-31665634 | Msa0018690:CDS | 35.0% |
| !!! | TGATGATGGTTATGGCTTTT+TGG | - | chr1_1:31665657-31665676 | None:intergenic | 35.0% |
| ! | CTTGAAAACCTTGTGTTTTG+AGG | + | chr1_1:31665977-31665996 | Msa0018690:intron | 35.0% |
| TGAAGGACTAAGTAAGAGAA+AGG | + | chr1_1:31666168-31666187 | Msa0018690:CDS | 35.0% | |
| AGAATAACCTCTTCTTCTTG+AGG | - | chr1_1:31666217-31666236 | None:intergenic | 35.0% | |
| TTCTAAGCAAAGAATCCCAA+AGG | + | chr1_1:31666233-31666252 | Msa0018690:CDS | 35.0% | |
| GTAACTATGCTTCTTCCTTT+GGG | - | chr1_1:31666251-31666270 | None:intergenic | 35.0% | |
| TGTAACTATGCTTCTTCCTT+TGG | - | chr1_1:31666252-31666271 | None:intergenic | 35.0% | |
| ! | AAGAAGCATAGTTACAACTC+TGG | + | chr1_1:31666256-31666275 | Msa0018690:CDS | 35.0% |
| CATATCAAGATCTCAGAAAG+TGG | - | chr1_1:31666358-31666377 | None:intergenic | 35.0% | |
| GATCTCGTTCACTAGCATTA+TGG | - | chr1_1:31665806-31665825 | None:intergenic | 40.0% | |
| TCGTTCACTTCTTCCTGTTT+CGG | + | chr1_1:31665860-31665879 | Msa0018690:CDS | 40.0% | |
| TCTTCCTGTTTCGGATCAAA+TGG | + | chr1_1:31665869-31665888 | Msa0018690:CDS | 40.0% | |
| AGTACCATTTGATCCGAAAC+AGG | - | chr1_1:31665876-31665895 | None:intergenic | 40.0% | |
| !!! | ACCTTGTGTTTTGAGGAGTA+TGG | + | chr1_1:31665984-31666003 | Msa0018690:intron | 40.0% |
| ACCATACTCCTCAAAACACA+AGG | - | chr1_1:31665988-31666007 | None:intergenic | 40.0% | |
| AGGACTGTTGAAATTGTTGC+TGG | - | chr1_1:31666118-31666137 | None:intergenic | 40.0% | |
| ACTTTCTCCTCAAGAAGAAG+AGG | + | chr1_1:31666207-31666226 | Msa0018690:CDS | 40.0% | |
| TCTCAGAAAGTGGAATCTTG+TGG | - | chr1_1:31666348-31666367 | None:intergenic | 40.0% | |
| CTTTCTTCATCTCAGACCTT+TGG | + | chr1_1:31666409-31666428 | Msa0018690:CDS | 40.0% | |
| GATCTCGTTCACTAGCATTA+TGG | - | chr1_1:31665806-31665825 | None:intergenic | 40.0% | |
| TCGTTCACTTCTTCCTGTTT+CGG | + | chr1_1:31665860-31665879 | Msa0018690:CDS | 40.0% | |
| TCTTCCTGTTTCGGATCAAA+TGG | + | chr1_1:31665869-31665888 | Msa0018690:CDS | 40.0% | |
| AGTACCATTTGATCCGAAAC+AGG | - | chr1_1:31665876-31665895 | None:intergenic | 40.0% | |
| !!! | ACCTTGTGTTTTGAGGAGTA+TGG | + | chr1_1:31665984-31666003 | Msa0018690:intron | 40.0% |
| ACCATACTCCTCAAAACACA+AGG | - | chr1_1:31665988-31666007 | None:intergenic | 40.0% | |
| AGGACTGTTGAAATTGTTGC+TGG | - | chr1_1:31666118-31666137 | None:intergenic | 40.0% | |
| ACTTTCTCCTCAAGAAGAAG+AGG | + | chr1_1:31666207-31666226 | Msa0018690:CDS | 40.0% | |
| TCTCAGAAAGTGGAATCTTG+TGG | - | chr1_1:31666348-31666367 | None:intergenic | 40.0% | |
| CTTTCTTCATCTCAGACCTT+TGG | + | chr1_1:31666409-31666428 | Msa0018690:CDS | 40.0% | |
| AACAATTGGATGGCCCTTTG+AGG | + | chr1_1:31665629-31665648 | Msa0018690:CDS | 45.0% | |
| !!! | TGGCTTTTTGGCTCCTCAAA+GGG | - | chr1_1:31665645-31665664 | None:intergenic | 45.0% |
| !!! | ATGGCTTTTTGGCTCCTCAA+AGG | - | chr1_1:31665646-31665665 | None:intergenic | 45.0% |
| ! | TCACTGCTTGTGGATTGTGA+GGG | - | chr1_1:31665759-31665778 | None:intergenic | 45.0% |
| CAAGCTTCTTGACCATGCTA+AGG | - | chr1_1:31665782-31665801 | None:intergenic | 45.0% | |
| AGGAAGAAGTGAACGAAGTG+AGG | - | chr1_1:31665856-31665875 | None:intergenic | 45.0% | |
| TCTTCATCTCAGACCTTTGG+AGG | + | chr1_1:31666412-31666431 | Msa0018690:CDS | 45.0% | |
| CTTCATCTCAGACCTTTGGA+GGG | + | chr1_1:31666413-31666432 | Msa0018690:CDS | 45.0% | |
| ATAGAAGACTCTCCCTCCAA+AGG | - | chr1_1:31666428-31666447 | None:intergenic | 45.0% | |
| AACAATTGGATGGCCCTTTG+AGG | + | chr1_1:31665629-31665648 | Msa0018690:CDS | 45.0% | |
| !!! | TGGCTTTTTGGCTCCTCAAA+GGG | - | chr1_1:31665645-31665664 | None:intergenic | 45.0% |
| !!! | ATGGCTTTTTGGCTCCTCAA+AGG | - | chr1_1:31665646-31665665 | None:intergenic | 45.0% |
| ! | TCACTGCTTGTGGATTGTGA+GGG | - | chr1_1:31665759-31665778 | None:intergenic | 45.0% |
| CAAGCTTCTTGACCATGCTA+AGG | - | chr1_1:31665782-31665801 | None:intergenic | 45.0% | |
| AGGAAGAAGTGAACGAAGTG+AGG | - | chr1_1:31665856-31665875 | None:intergenic | 45.0% | |
| TCTTCATCTCAGACCTTTGG+AGG | + | chr1_1:31666412-31666431 | Msa0018690:CDS | 45.0% | |
| CTTCATCTCAGACCTTTGGA+GGG | + | chr1_1:31666413-31666432 | Msa0018690:CDS | 45.0% | |
| ATAGAAGACTCTCCCTCCAA+AGG | - | chr1_1:31666428-31666447 | None:intergenic | 45.0% | |
| ! | GTCACTGCTTGTGGATTGTG+AGG | - | chr1_1:31665760-31665779 | None:intergenic | 50.0% |
| CATGCTAAGGTCACTGCTTG+TGG | - | chr1_1:31665769-31665788 | None:intergenic | 50.0% | |
| CACAAGCAGTGACCTTAGCA+TGG | + | chr1_1:31665767-31665786 | Msa0018690:CDS | 50.0% | |
| ! | GTCACTGCTTGTGGATTGTG+AGG | - | chr1_1:31665760-31665779 | None:intergenic | 50.0% |
| CATGCTAAGGTCACTGCTTG+TGG | - | chr1_1:31665769-31665788 | None:intergenic | 50.0% | |
| CACAAGCAGTGACCTTAGCA+TGG | + | chr1_1:31665767-31665786 | Msa0018690:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_1 | gene | 31665600 | 31666471 | 31665600 | ID=Msa0018690;Name=Msa0018690 |
| chr1_1 | mRNA | 31665600 | 31666471 | 31665600 | ID=Msa0018690-mRNA-1;Parent=Msa0018690;Name=Msa0018690-mRNA-1;_AED=0.08;_eAED=0.08;_QI=0|0|0|1|1|1|2|0|220 |
| chr1_1 | exon | 31665600 | 31665890 | 31665600 | ID=Msa0018690-mRNA-1:exon:10384;Parent=Msa0018690-mRNA-1 |
| chr1_1 | exon | 31666100 | 31666471 | 31666100 | ID=Msa0018690-mRNA-1:exon:10385;Parent=Msa0018690-mRNA-1 |
| chr1_1 | CDS | 31665600 | 31665890 | 31665600 | ID=Msa0018690-mRNA-1:cds;Parent=Msa0018690-mRNA-1 |
| chr1_1 | CDS | 31666100 | 31666471 | 31666100 | ID=Msa0018690-mRNA-1:cds;Parent=Msa0018690-mRNA-1 |
| Gene Sequence |
| Protein sequence |
