AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0112640


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0112640 MtrunA17_Chr1g0170581 92.713 247 18 0 1 247 1 247 8.91e-168 462
Msa0112640 MtrunA17_Chr1g0170571 88.525 244 20 1 1 244 1 236 3.69e-147 410
Msa0112640 MtrunA17_Chr1g0170591 83.197 244 33 1 1 244 1 236 1.25e-146 409
Msa0112640 MtrunA17_Chr7g0256671 56.972 251 89 7 1 241 1 242 9.58e-65 202
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0112640 AT3G56970.1 41.365 249 135 5 1 240 1 247 1.93e-55 178
Msa0112640 AT3G56980.1 39.194 273 116 6 1 242 1 254 1.82e-46 155
Msa0112640 AT2G41240.1 38.000 250 135 5 1 242 1 238 3.96e-45 151
Msa0112640 AT2G41240.2 37.600 250 135 6 1 242 1 237 1.57e-42 145
Msa0112640 AT5G04150.2 45.161 155 83 1 65 219 86 238 1.88e-35 126
Msa0112640 AT5G04150.1 43.429 175 94 3 65 237 86 257 2.17e-35 127

Find 35 sgRNAs with CRISPR-Local

Find 122 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAACTTGTTTCTGTAATTC+TGG 0.201065 1_3:-27768714 None:intergenic
TCTTCATCTCAGACCTTTGC+AGG 0.241758 1_3:+27768978 Msa0112640:CDS
TGTAACTATGCTTCTTCCTT+TGG 0.251062 1_3:-27768815 None:intergenic
CTTCTATAATTTGCATTTCC+AGG 0.265122 1_3:+27769007 Msa0112640:CDS
GTAACTATGCTTCTTCCTTT+GGG 0.307422 1_3:-27768814 None:intergenic
AGGACTCTTGAAATTGTTGC+TGG 0.367920 1_3:-27768681 None:intergenic
GATCGCGTTCACTAGCATTA+TGG 0.375108 1_3:-27768370 None:intergenic
TCTTCCTGTTTCTGATCAAA+TGG 0.413993 1_3:+27768436 Msa0112640:CDS
AGTACCATTTGATCAGAAAC+AGG 0.414053 1_3:-27768440 None:intergenic
ATGAATTCTGATGATGGTTA+TGG 0.420513 1_3:-27768229 None:intergenic
AACAATTGGATGGCCCTTTG+AGG 0.464251 1_3:+27768196 Msa0112640:CDS
TGAAGGACTAAGTAAGAGAA+AGG 0.469978 1_3:+27768734 Msa0112640:CDS
CACATCAAGATCTCAGAAAG+TGG 0.497267 1_3:-27768921 None:intergenic
AGAATAACCTCTTCTTCTTG+AGG 0.516991 1_3:-27768780 None:intergenic
TGTAGAATGAATTCTGATGA+TGG 0.522101 1_3:-27768235 None:intergenic
CATGCTAAGGTCACTGCTTG+TGG 0.529098 1_3:-27768333 None:intergenic
ACAAATTATTGATCTTCTTG+CGG 0.529574 1_3:-27768394 None:intergenic
CTTCATCTCAGACCTTTGCA+GGG 0.548412 1_3:+27768979 Msa0112640:CDS
AAGAAGCATAGTTACAACTC+TGG 0.551749 1_3:+27768822 Msa0112640:CDS
ACTTTATCCTCAAGAAGAAG+AGG 0.554683 1_3:+27768773 Msa0112640:CDS
AGTGTAACATGAAATCTGAA+TGG 0.555088 1_3:-27768886 None:intergenic
GAATTACAGAAACAAGTTGA+AGG 0.556198 1_3:+27768717 Msa0112640:CDS
CATCCAATTGTTGAAAACAT+AGG 0.558093 1_3:-27768185 None:intergenic
TCTCAGAAAGTGGAATCTTG+TGG 0.569417 1_3:-27768911 None:intergenic
GCTCTTATCAATAATCGAGA+AGG 0.576892 1_3:+27769579 Msa0112640:CDS
TTGATGTGTTTAGAAAATGA+TGG 0.577722 1_3:+27768936 Msa0112640:CDS
ATAGAAGACTCTCCCTGCAA+AGG 0.579465 1_3:-27768991 None:intergenic
CCAATTGTTGAAAACATAGG+AGG 0.582074 1_3:-27768182 None:intergenic
GTCACTGCTTGTGGATTGTG+AGG 0.596424 1_3:-27768324 None:intergenic
AGGAAGAAGTGAACGAAGTG+AGG 0.599839 1_3:-27768420 None:intergenic
CAAGCTTCTTGACCATGCTA+AGG 0.604223 1_3:-27768346 None:intergenic
CACAAGCAGTGACCTTAGCA+TGG 0.613188 1_3:+27768334 Msa0112640:CDS
TTCTAAGCAAAGAATCCCAA+AGG 0.620328 1_3:+27768799 Msa0112640:CDS
TTATCAATAATCGAGAAGGA+AGG 0.636620 1_3:+27769583 Msa0112640:CDS
TCACTGCTTGTGGATTGTGA+GGG 0.698792 1_3:-27768323 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTCACAAATGTTAAATT+TGG + chr1_3:27769256-27769275 Msa0112640:intron 15.0%
!! TATACAAATAATTGATTACT+TGG + chr1_3:27769400-27769419 Msa0112640:intron 15.0%
!! ATACAAATAATTGATTACTT+GGG + chr1_3:27769401-27769420 Msa0112640:intron 15.0%
!!! ATTTTCACAAATGTTAAATT+TGG + chr1_3:27769256-27769275 Msa0112640:intron 15.0%
!! TATACAAATAATTGATTACT+TGG + chr1_3:27769400-27769419 Msa0112640:intron 15.0%
!! ATACAAATAATTGATTACTT+GGG + chr1_3:27769401-27769420 Msa0112640:intron 15.0%
!! GATCATACATAATTATGATT+TGG + chr1_3:27769177-27769196 Msa0112640:intron 20.0%
!! TTAAAAACATGTAAGAAGTA+AGG + chr1_3:27769339-27769358 Msa0112640:intron 20.0%
!!! ACACAAAAAGCTTTTAATTA+AGG - chr1_3:27769506-27769525 None:intergenic 20.0%
!! GATCATACATAATTATGATT+TGG + chr1_3:27769177-27769196 Msa0112640:intron 20.0%
!! TTAAAAACATGTAAGAAGTA+AGG + chr1_3:27769339-27769358 Msa0112640:intron 20.0%
!!! ACACAAAAAGCTTTTAATTA+AGG - chr1_3:27769506-27769525 None:intergenic 20.0%
!!! TAATTTTCATGATCAAGTTG+AGG + chr1_3:27768280-27768299 Msa0112640:CDS 25.0%
! ACAAATTATTGATCTTCTTG+CGG - chr1_3:27768397-27768416 None:intergenic 25.0%
! ACAATTAGAAACAGAAAGAA+GGG - chr1_3:27768474-27768493 None:intergenic 25.0%
! TCTAATTGTCAATAATGCTT+TGG + chr1_3:27768485-27768504 Msa0112640:intron 25.0%
!! GTAATTCTGGTATGTATTTT+AGG - chr1_3:27768704-27768723 None:intergenic 25.0%
! TTGATGTGTTTAGAAAATGA+TGG + chr1_3:27768936-27768955 Msa0112640:CDS 25.0%
!!! TAATTTTCATGATCAAGTTG+AGG + chr1_3:27768280-27768299 Msa0112640:CDS 25.0%
! ACAAATTATTGATCTTCTTG+CGG - chr1_3:27768397-27768416 None:intergenic 25.0%
! ACAATTAGAAACAGAAAGAA+GGG - chr1_3:27768474-27768493 None:intergenic 25.0%
! TCTAATTGTCAATAATGCTT+TGG + chr1_3:27768485-27768504 Msa0112640:intron 25.0%
!! GTAATTCTGGTATGTATTTT+AGG - chr1_3:27768704-27768723 None:intergenic 25.0%
! TTGATGTGTTTAGAAAATGA+TGG + chr1_3:27768936-27768955 Msa0112640:CDS 25.0%
CATCCAATTGTTGAAAACAT+AGG - chr1_3:27768188-27768207 None:intergenic 30.0%
! CTATGTTTTCAACAATTGGA+TGG + chr1_3:27768186-27768205 Msa0112640:CDS 30.0%
!! ATGAATTCTGATGATGGTTA+TGG - chr1_3:27768232-27768251 None:intergenic 30.0%
TGTAGAATGAATTCTGATGA+TGG - chr1_3:27768238-27768257 None:intergenic 30.0%
GACAATTAGAAACAGAAAGA+AGG - chr1_3:27768475-27768494 None:intergenic 30.0%
!!! TCTAATTGTGATGTCGTTTT+TGG + chr1_3:27768582-27768601 Msa0112640:intron 30.0%
!! TCAACTTGTTTCTGTAATTC+TGG - chr1_3:27768717-27768736 None:intergenic 30.0%
! GAATTACAGAAACAAGTTGA+AGG + chr1_3:27768717-27768736 Msa0112640:CDS 30.0%
!! GTTTTGTAGTTTCAAGTAGT+AGG + chr1_3:27768844-27768863 Msa0112640:CDS 30.0%
AGTGTAACATGAAATCTGAA+TGG - chr1_3:27768889-27768908 None:intergenic 30.0%
! CTTCTATAATTTGCATTTCC+AGG + chr1_3:27769007-27769026 Msa0112640:CDS 30.0%
GATGTATAGACTCATAAAAG+AGG + chr1_3:27769465-27769484 Msa0112640:intron 30.0%
TTATCAATAATCGAGAAGGA+AGG + chr1_3:27769583-27769602 Msa0112640:CDS 30.0%
CATCCAATTGTTGAAAACAT+AGG - chr1_3:27768188-27768207 None:intergenic 30.0%
! CTATGTTTTCAACAATTGGA+TGG + chr1_3:27768186-27768205 Msa0112640:CDS 30.0%
!! ATGAATTCTGATGATGGTTA+TGG - chr1_3:27768232-27768251 None:intergenic 30.0%
TGTAGAATGAATTCTGATGA+TGG - chr1_3:27768238-27768257 None:intergenic 30.0%
GACAATTAGAAACAGAAAGA+AGG - chr1_3:27768475-27768494 None:intergenic 30.0%
!!! TCTAATTGTGATGTCGTTTT+TGG + chr1_3:27768582-27768601 Msa0112640:intron 30.0%
!! TCAACTTGTTTCTGTAATTC+TGG - chr1_3:27768717-27768736 None:intergenic 30.0%
! GAATTACAGAAACAAGTTGA+AGG + chr1_3:27768717-27768736 Msa0112640:CDS 30.0%
!! GTTTTGTAGTTTCAAGTAGT+AGG + chr1_3:27768844-27768863 Msa0112640:CDS 30.0%
AGTGTAACATGAAATCTGAA+TGG - chr1_3:27768889-27768908 None:intergenic 30.0%
! CTTCTATAATTTGCATTTCC+AGG + chr1_3:27769007-27769026 Msa0112640:CDS 30.0%
GATGTATAGACTCATAAAAG+AGG + chr1_3:27769465-27769484 Msa0112640:intron 30.0%
TTATCAATAATCGAGAAGGA+AGG + chr1_3:27769583-27769602 Msa0112640:CDS 30.0%
CCAATTGTTGAAAACATAGG+AGG - chr1_3:27768185-27768204 None:intergenic 35.0%
! CCTCCTATGTTTTCAACAAT+TGG + chr1_3:27768182-27768201 Msa0112640:CDS 35.0%
!!! TGATGATGGTTATGGCTTTT+TGG - chr1_3:27768224-27768243 None:intergenic 35.0%
!! TCTTCCTGTTTCTGATCAAA+TGG + chr1_3:27768436-27768455 Msa0112640:CDS 35.0%
AGTACCATTTGATCAGAAAC+AGG - chr1_3:27768443-27768462 None:intergenic 35.0%
! CTTGAAAACCTTGTGTTTTG+AGG + chr1_3:27768543-27768562 Msa0112640:intron 35.0%
TGAAGGACTAAGTAAGAGAA+AGG + chr1_3:27768734-27768753 Msa0112640:CDS 35.0%
ACTTTATCCTCAAGAAGAAG+AGG + chr1_3:27768773-27768792 Msa0112640:CDS 35.0%
AGAATAACCTCTTCTTCTTG+AGG - chr1_3:27768783-27768802 None:intergenic 35.0%
TTCTAAGCAAAGAATCCCAA+AGG + chr1_3:27768799-27768818 Msa0112640:CDS 35.0%
GTAACTATGCTTCTTCCTTT+GGG - chr1_3:27768817-27768836 None:intergenic 35.0%
TGTAACTATGCTTCTTCCTT+TGG - chr1_3:27768818-27768837 None:intergenic 35.0%
! AAGAAGCATAGTTACAACTC+TGG + chr1_3:27768822-27768841 Msa0112640:CDS 35.0%
! AAGCTTTTTGTGTTTACTGC+AGG + chr1_3:27769513-27769532 Msa0112640:intron 35.0%
GCTCTTATCAATAATCGAGA+AGG + chr1_3:27769579-27769598 Msa0112640:CDS 35.0%
CCAATTGTTGAAAACATAGG+AGG - chr1_3:27768185-27768204 None:intergenic 35.0%
! CCTCCTATGTTTTCAACAAT+TGG + chr1_3:27768182-27768201 Msa0112640:CDS 35.0%
!!! TGATGATGGTTATGGCTTTT+TGG - chr1_3:27768224-27768243 None:intergenic 35.0%
!! TCTTCCTGTTTCTGATCAAA+TGG + chr1_3:27768436-27768455 Msa0112640:CDS 35.0%
AGTACCATTTGATCAGAAAC+AGG - chr1_3:27768443-27768462 None:intergenic 35.0%
! CTTGAAAACCTTGTGTTTTG+AGG + chr1_3:27768543-27768562 Msa0112640:intron 35.0%
TGAAGGACTAAGTAAGAGAA+AGG + chr1_3:27768734-27768753 Msa0112640:CDS 35.0%
ACTTTATCCTCAAGAAGAAG+AGG + chr1_3:27768773-27768792 Msa0112640:CDS 35.0%
AGAATAACCTCTTCTTCTTG+AGG - chr1_3:27768783-27768802 None:intergenic 35.0%
TTCTAAGCAAAGAATCCCAA+AGG + chr1_3:27768799-27768818 Msa0112640:CDS 35.0%
GTAACTATGCTTCTTCCTTT+GGG - chr1_3:27768817-27768836 None:intergenic 35.0%
TGTAACTATGCTTCTTCCTT+TGG - chr1_3:27768818-27768837 None:intergenic 35.0%
! AAGAAGCATAGTTACAACTC+TGG + chr1_3:27768822-27768841 Msa0112640:CDS 35.0%
! AAGCTTTTTGTGTTTACTGC+AGG + chr1_3:27769513-27769532 Msa0112640:intron 35.0%
GCTCTTATCAATAATCGAGA+AGG + chr1_3:27769579-27769598 Msa0112640:CDS 35.0%
!!! ACCTTGTGTTTTGAGGAGTA+TGG + chr1_3:27768550-27768569 Msa0112640:intron 40.0%
ACCATACTCCTCAAAACACA+AGG - chr1_3:27768554-27768573 None:intergenic 40.0%
AGGACTCTTGAAATTGTTGC+TGG - chr1_3:27768684-27768703 None:intergenic 40.0%
TCTCAGAAAGTGGAATCTTG+TGG - chr1_3:27768914-27768933 None:intergenic 40.0%
CACATCAAGATCTCAGAAAG+TGG - chr1_3:27768924-27768943 None:intergenic 40.0%
CCTAGTGAAATTAACACACC+TGG - chr1_3:27769028-27769047 None:intergenic 40.0%
CCAGGTGTGTTAATTTCACT+AGG + chr1_3:27769025-27769044 Msa0112640:intron 40.0%
! CTTTTTGTGTTTACTGCAGG+TGG + chr1_3:27769516-27769535 Msa0112640:intron 40.0%
!!! ACCTTGTGTTTTGAGGAGTA+TGG + chr1_3:27768550-27768569 Msa0112640:intron 40.0%
ACCATACTCCTCAAAACACA+AGG - chr1_3:27768554-27768573 None:intergenic 40.0%
AGGACTCTTGAAATTGTTGC+TGG - chr1_3:27768684-27768703 None:intergenic 40.0%
TCTCAGAAAGTGGAATCTTG+TGG - chr1_3:27768914-27768933 None:intergenic 40.0%
CACATCAAGATCTCAGAAAG+TGG - chr1_3:27768924-27768943 None:intergenic 40.0%
CCTAGTGAAATTAACACACC+TGG - chr1_3:27769028-27769047 None:intergenic 40.0%
CCAGGTGTGTTAATTTCACT+AGG + chr1_3:27769025-27769044 Msa0112640:intron 40.0%
! CTTTTTGTGTTTACTGCAGG+TGG + chr1_3:27769516-27769535 Msa0112640:intron 40.0%
AACAATTGGATGGCCCTTTG+AGG + chr1_3:27768196-27768215 Msa0112640:CDS 45.0%
!!! TGGCTTTTTGGCTCCTCAAA+GGG - chr1_3:27768212-27768231 None:intergenic 45.0%
!!! ATGGCTTTTTGGCTCCTCAA+AGG - chr1_3:27768213-27768232 None:intergenic 45.0%
! TCACTGCTTGTGGATTGTGA+GGG - chr1_3:27768326-27768345 None:intergenic 45.0%
CAAGCTTCTTGACCATGCTA+AGG - chr1_3:27768349-27768368 None:intergenic 45.0%
GATCGCGTTCACTAGCATTA+TGG - chr1_3:27768373-27768392 None:intergenic 45.0%
AGGAAGAAGTGAACGAAGTG+AGG - chr1_3:27768423-27768442 None:intergenic 45.0%
TCTTCATCTCAGACCTTTGC+AGG + chr1_3:27768978-27768997 Msa0112640:CDS 45.0%
CTTCATCTCAGACCTTTGCA+GGG + chr1_3:27768979-27768998 Msa0112640:CDS 45.0%
ATAGAAGACTCTCCCTGCAA+AGG - chr1_3:27768994-27769013 None:intergenic 45.0%
AACAATTGGATGGCCCTTTG+AGG + chr1_3:27768196-27768215 Msa0112640:CDS 45.0%
!!! TGGCTTTTTGGCTCCTCAAA+GGG - chr1_3:27768212-27768231 None:intergenic 45.0%
!!! ATGGCTTTTTGGCTCCTCAA+AGG - chr1_3:27768213-27768232 None:intergenic 45.0%
! TCACTGCTTGTGGATTGTGA+GGG - chr1_3:27768326-27768345 None:intergenic 45.0%
CAAGCTTCTTGACCATGCTA+AGG - chr1_3:27768349-27768368 None:intergenic 45.0%
GATCGCGTTCACTAGCATTA+TGG - chr1_3:27768373-27768392 None:intergenic 45.0%
AGGAAGAAGTGAACGAAGTG+AGG - chr1_3:27768423-27768442 None:intergenic 45.0%
TCTTCATCTCAGACCTTTGC+AGG + chr1_3:27768978-27768997 Msa0112640:CDS 45.0%
CTTCATCTCAGACCTTTGCA+GGG + chr1_3:27768979-27768998 Msa0112640:CDS 45.0%
ATAGAAGACTCTCCCTGCAA+AGG - chr1_3:27768994-27769013 None:intergenic 45.0%
! GTCACTGCTTGTGGATTGTG+AGG - chr1_3:27768327-27768346 None:intergenic 50.0%
CATGCTAAGGTCACTGCTTG+TGG - chr1_3:27768336-27768355 None:intergenic 50.0%
CACAAGCAGTGACCTTAGCA+TGG + chr1_3:27768334-27768353 Msa0112640:CDS 50.0%
! GTCACTGCTTGTGGATTGTG+AGG - chr1_3:27768327-27768346 None:intergenic 50.0%
CATGCTAAGGTCACTGCTTG+TGG - chr1_3:27768336-27768355 None:intergenic 50.0%
CACAAGCAGTGACCTTAGCA+TGG + chr1_3:27768334-27768353 Msa0112640:CDS 50.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 27768167 27769624 27768167 ID=Msa0112640;Name=Msa0112640
chr1_3 mRNA 27768167 27769624 27768167 ID=Msa0112640-mRNA-1;Parent=Msa0112640;Name=Msa0112640-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0|0|0|1|1|1|3|0|247
chr1_3 exon 27768167 27768457 27768167 ID=Msa0112640-mRNA-1:exon:9203;Parent=Msa0112640-mRNA-1
chr1_3 exon 27768666 27769028 27768666 ID=Msa0112640-mRNA-1:exon:9204;Parent=Msa0112640-mRNA-1
chr1_3 exon 27769535 27769624 27769535 ID=Msa0112640-mRNA-1:exon:9205;Parent=Msa0112640-mRNA-1
chr1_3 CDS 27768167 27768457 27768167 ID=Msa0112640-mRNA-1:cds;Parent=Msa0112640-mRNA-1
chr1_3 CDS 27768666 27769028 27768666 ID=Msa0112640-mRNA-1:cds;Parent=Msa0112640-mRNA-1
chr1_3 CDS 27769535 27769624 27769535 ID=Msa0112640-mRNA-1:cds;Parent=Msa0112640-mRNA-1
Gene Sequence

>Msa0112640

ATGTTAGCGATATCTCCTCCTATGTTTTCAACAATTGGATGGCCCTTTGAGGAGCCAAAAAGCCATAACCATCATCAGAATTCATTCTACAAAGACACTGTTGATCAATTATTTAATTTTCATGATCAAGTTGAGGCAGAAATTAATTCAACAGATCCCTCACAATCCACAAGCAGTGACCTTAGCATGGTCAAGAAGCTTGTCCATAATGCTAGTGAACGCGATCGCCGCAAGAAGATCAATAATTTGTATTCCTCACTTCGTTCACTTCTTCCTGTTTCTGATCAAATGAAGAAATTGAGCATTCCAGCAACAATTTCAAGAGTCCTAAAATACATACCAGAATTACAGAAACAAGTTGAAGGACTAAGTAAGAGAAAGGATGAGATCTTATTGAGACTTTATCCTCAAGAAGAAGAGGTTATTCTAAGCAAAGAATCCCAAAGGAAGAAGCATAGTTACAACTCTGGTTTTGTAGTTTCAAGTAGTAGGCTCAATGATAGTGAAATTACCATTCAGATTTCATGTTACACTGTCCACAAGATTCCACTTTCTGAGATCTTGATGTGTTTAGAAAATGATGGTTTTTTGCTTCTTAATCTTTCTTCATCTCAGACCTTTGCAGGGAGAGTCTTCTATAATTTGCATTTCCAGGTGGATAAAGCACAGAGATTAGAATCTCATATTCTAAATGAGAAGCTCTTATCAATAATCGAGAAGGAAGGAGAGTTTTTAAAACAATAA

Protein sequence

>Msa0112640

MLAISPPMFSTIGWPFEEPKSHNHHQNSFYKDTVDQLFNFHDQVEAEINSTDPSQSTSSDLSMVKKLVHNASERDRRKKINNLYSSLRSLLPVSDQMKKLSIPATISRVLKYIPELQKQVEGLSKRKDEILLRLYPQEEEVILSKESQRKKHSYNSGFVVSSSRLNDSEITIQISCYTVHKIPLSEILMCLENDGFLLLNLSSSQTFAGRVFYNLHFQVDKAQRLESHILNEKLLSIIEKEGEFLKQ*