Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0022350 | Msa1349090 | 0.934653 | 2.892283e-96 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0022350 | MtrunA17_Chr2g0293221 | 89.091 | 110 | 12 | 0 | 1 | 110 | 1 | 110 | 5.13e-64 | 198 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 25 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAGAATAGGAGAAGAAAAT+AGG | 0.217982 | 1_1:+41915427 | None:intergenic |
| GAATCTGTAGAGCGGGAAAT+TGG | 0.230915 | 1_1:-41915357 | Msa0022350:CDS |
| TTCTGATATTAAACGTTATA+AGG | 0.239982 | 1_1:-41915214 | Msa0022350:CDS |
| GGTTCAGATGATGAAGAATC+AGG | 0.362544 | 1_1:-41915313 | Msa0022350:CDS |
| TCTACGGCATTTGAAGGTTT+CGG | 0.374083 | 1_1:+41915378 | None:intergenic |
| CGCATCGCGTGATGCAGAAT+AGG | 0.405986 | 1_1:+41915414 | None:intergenic |
| CAGAATAGGAGAAGAAAATA+GGG | 0.432768 | 1_1:+41915428 | None:intergenic |
| TTATAAGGATCAAATTTCTC+TGG | 0.442275 | 1_1:-41915199 | Msa0022350:intron |
| AATCTGTAGAGCGGGAAATT+GGG | 0.454600 | 1_1:-41915356 | Msa0022350:CDS |
| ACAGATTCTACGGCATTTGA+AGG | 0.458023 | 1_1:+41915372 | None:intergenic |
| AGCTAAGGCTGATTTGATGA+TGG | 0.458362 | 1_1:-41915041 | Msa0022350:CDS |
| GGTGAAGGTTTCGGATGAAG+AGG | 0.497928 | 1_1:+41915464 | None:intergenic |
| TTCCCGCTCTACAGATTCTA+CGG | 0.498936 | 1_1:+41915362 | None:intergenic |
| TGCCGTAGAATCTGTAGAGC+GGG | 0.500688 | 1_1:-41915364 | Msa0022350:CDS |
| AAAATTGCAGAATCAACGTT+GGG | 0.501820 | 1_1:-41915238 | Msa0022350:CDS |
| GGATGAAGAGGAGGCGGTGA+AGG | 0.514242 | 1_1:+41915476 | None:intergenic |
| GCTAAGGCTGATTTGATGAT+GGG | 0.544049 | 1_1:-41915040 | Msa0022350:CDS |
| TGAAGAGGAGGCGGTGAAGG+AGG | 0.544990 | 1_1:+41915479 | None:intergenic |
| ACGGTTGCTTGAGGAAGCTA+AGG | 0.548023 | 1_1:-41915056 | Msa0022350:CDS |
| TTGTAGAAAGTTCTGTTGAT+CGG | 0.548922 | 1_1:+41915269 | None:intergenic |
| TAAAATTGCAGAATCAACGT+TGG | 0.578447 | 1_1:-41915239 | Msa0022350:CDS |
| GGTTTCGGATGAAGAGGAGG+CGG | 0.608657 | 1_1:+41915470 | None:intergenic |
| GAAGGTTTCGGATGAAGAGG+AGG | 0.626161 | 1_1:+41915467 | None:intergenic |
| AGAGAAGAAACGGTTGCTTG+AGG | 0.641429 | 1_1:-41915065 | Msa0022350:CDS |
| ATGCCGTAGAATCTGTAGAG+CGG | 0.698197 | 1_1:-41915365 | Msa0022350:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTGATTAATTTCATTTTT+AGG | - | chr1_1:41915309-41915328 | Msa0022350:CDS | 10.0% |
| !!! | ATTGATTAATTTCATTTTTA+GGG | - | chr1_1:41915310-41915329 | Msa0022350:CDS | 10.0% |
| !! | AAAAAAAATTGAAAAAAGAA+TGG | - | chr1_1:41915339-41915358 | Msa0022350:CDS | 10.0% |
| !!! | AAAAAGAATGGTTTTTTTAA+TGG | - | chr1_1:41915351-41915370 | Msa0022350:CDS | 15.0% |
| !!! | AAAAGAATGGTTTTTTTAAT+GGG | - | chr1_1:41915352-41915371 | Msa0022350:CDS | 15.0% |
| !!! | AAAATCTTCTATTTTGAAAT+GGG | - | chr1_1:41915478-41915497 | Msa0022350:five_prime_UTR | 15.0% |
| !! | TTCTGATATTAAACGTTATA+AGG | - | chr1_1:41915269-41915288 | Msa0022350:CDS | 20.0% |
| !!! | GAAAATCTTCTATTTTGAAA+TGG | - | chr1_1:41915477-41915496 | Msa0022350:five_prime_UTR | 20.0% |
| !!! | AAATAGGGCTAATTTGTTTT+AGG | + | chr1_1:41915043-41915062 | None:intergenic | 25.0% |
| ! | GAAAAAAATATTCAGCACTT+TGG | - | chr1_1:41915149-41915168 | Msa0022350:intron | 25.0% |
| ! | TTATAAGGATCAAATTTCTC+TGG | - | chr1_1:41915284-41915303 | Msa0022350:CDS | 25.0% |
| !!! | TGATTTTTGAAGAGAAGAAA+CGG | - | chr1_1:41915408-41915427 | Msa0022350:exon | 25.0% |
| !!! | TTTGTTTTAGGTGAAGGTTT+CGG | + | chr1_1:41915031-41915050 | None:intergenic | 30.0% |
| CAGAATAGGAGAAGAAAATA+GGG | + | chr1_1:41915058-41915077 | None:intergenic | 30.0% | |
| TTGTAGAAAGTTCTGTTGAT+CGG | + | chr1_1:41915217-41915236 | None:intergenic | 30.0% | |
| TAAAATTGCAGAATCAACGT+TGG | - | chr1_1:41915244-41915263 | Msa0022350:CDS | 30.0% | |
| AAAATTGCAGAATCAACGTT+GGG | - | chr1_1:41915245-41915264 | Msa0022350:CDS | 30.0% | |
| !!! | GGCTAATTTGTTTTAGGTGA+AGG | + | chr1_1:41915037-41915056 | None:intergenic | 35.0% |
| GCAGAATAGGAGAAGAAAAT+AGG | + | chr1_1:41915059-41915078 | None:intergenic | 35.0% | |
| TCTACGGCATTTGAAGGTTT+CGG | + | chr1_1:41915108-41915127 | None:intergenic | 40.0% | |
| ACAGATTCTACGGCATTTGA+AGG | + | chr1_1:41915114-41915133 | None:intergenic | 40.0% | |
| AATCTGTAGAGCGGGAAATT+GGG | - | chr1_1:41915127-41915146 | Msa0022350:intron | 40.0% | |
| GGTTCAGATGATGAAGAATC+AGG | - | chr1_1:41915170-41915189 | Msa0022350:intron | 40.0% | |
| !! | AGCTAAGGCTGATTTGATGA+TGG | - | chr1_1:41915442-41915461 | Msa0022350:five_prime_UTR | 40.0% |
| !! | GCTAAGGCTGATTTGATGAT+GGG | - | chr1_1:41915443-41915462 | Msa0022350:five_prime_UTR | 40.0% |
| ATGCCGTAGAATCTGTAGAG+CGG | - | chr1_1:41915118-41915137 | Msa0022350:intron | 45.0% | |
| TTCCCGCTCTACAGATTCTA+CGG | + | chr1_1:41915124-41915143 | None:intergenic | 45.0% | |
| GAATCTGTAGAGCGGGAAAT+TGG | - | chr1_1:41915126-41915145 | Msa0022350:intron | 45.0% | |
| AGAGAAGAAACGGTTGCTTG+AGG | - | chr1_1:41915418-41915437 | Msa0022350:exon | 45.0% | |
| GAAGGTTTCGGATGAAGAGG+AGG | + | chr1_1:41915019-41915038 | None:intergenic | 50.0% | |
| GGTGAAGGTTTCGGATGAAG+AGG | + | chr1_1:41915022-41915041 | None:intergenic | 50.0% | |
| TGCCGTAGAATCTGTAGAGC+GGG | - | chr1_1:41915119-41915138 | Msa0022350:intron | 50.0% | |
| ACGGTTGCTTGAGGAAGCTA+AGG | - | chr1_1:41915427-41915446 | Msa0022350:five_prime_UTR | 50.0% | |
| GGTTTCGGATGAAGAGGAGG+CGG | + | chr1_1:41915016-41915035 | None:intergenic | 55.0% | |
| CGCATCGCGTGATGCAGAAT+AGG | + | chr1_1:41915072-41915091 | None:intergenic | 55.0% | |
| TGAAGAGGAGGCGGTGAAGG+AGG | + | chr1_1:41915007-41915026 | None:intergenic | 60.0% | |
| GGATGAAGAGGAGGCGGTGA+AGG | + | chr1_1:41915010-41915029 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_1 | gene | 41915000 | 41915505 | 41915000 | ID=Msa0022350;Name=Msa0022350 |
| chr1_1 | mRNA | 41915000 | 41915505 | 41915000 | ID=Msa0022350-mRNA-1;Parent=Msa0022350;Name=Msa0022350-mRNA-1;_AED=0.01;_eAED=0.01;_QI=87|1|0.5|1|1|1|2|0|110 |
| chr1_1 | exon | 41915000 | 41915110 | 41915000 | ID=Msa0022350-mRNA-1:exon:12343;Parent=Msa0022350-mRNA-1 |
| chr1_1 | exon | 41915200 | 41915505 | 41915200 | ID=Msa0022350-mRNA-1:exon:12342;Parent=Msa0022350-mRNA-1 |
| chr1_1 | five_prime_UTR | 41915419 | 41915505 | 41915419 | ID=Msa0022350-mRNA-1:five_prime_utr;Parent=Msa0022350-mRNA-1 |
| chr1_1 | CDS | 41915200 | 41915418 | 41915200 | ID=Msa0022350-mRNA-1:cds;Parent=Msa0022350-mRNA-1 |
| chr1_1 | CDS | 41915000 | 41915110 | 41915000 | ID=Msa0022350-mRNA-1:cds;Parent=Msa0022350-mRNA-1 |
| Gene Sequence |
| Protein sequence |
