Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0025910 | Msa0025920 | 0.829051 | 6.391176e-55 | -8.615850e-47 |
| Msa0025910 | Msa0073530 | 0.829702 | 4.440971e-55 | -8.615850e-47 |
| Msa0025910 | Msa0909120 | 0.815416 | 9.351211e-52 | -8.615850e-47 |
| Msa0025910 | Msa1438250 | 0.808967 | 2.387837e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 24 sgRNAs with CRISPR-Local
Find 110 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTATTGAAGTCCTGATTAT+TGG | 0.160633 | 1_1:+49530237 | Msa0025910:CDS |
| TATATCAGGGTCCAATAATC+AGG | 0.326397 | 1_1:-49530248 | None:intergenic |
| GACGGAGCTGCTGCAGAGAT+TGG | 0.357540 | 1_1:+49529501 | Msa0025910:CDS |
| CTGCTAGATCGGAGATGGGC+CGG | 0.378849 | 1_1:+49529478 | Msa0025910:CDS |
| CGGGGCTGCTAGATCGGAGA+TGG | 0.432256 | 1_1:+49529473 | Msa0025910:CDS |
| CTCATGCAAGAAACGGTTGA+TGG | 0.480355 | 1_1:+49530033 | Msa0025910:CDS |
| AAAAGATGTCCAATGTCAAA+TGG | 0.485689 | 1_1:+49529939 | Msa0025910:CDS |
| TGCTAGATCGGAGATGGGCC+GGG | 0.486001 | 1_1:+49529479 | Msa0025910:CDS |
| AGGTGACGGGGCTGCTAGAT+CGG | 0.496214 | 1_1:+49529467 | Msa0025910:CDS |
| GTGCATGATCATAGTATACT+TGG | 0.505626 | 1_1:-49529570 | None:intergenic |
| CTGAATATGTCGAAGTGAAA+GGG | 0.506298 | 1_1:+49529963 | Msa0025910:CDS |
| TTCCTCGCTCATGCAAGAAA+CGG | 0.534516 | 1_1:+49530026 | Msa0025910:CDS |
| TCTGAATATGTCGAAGTGAA+AGG | 0.539318 | 1_1:+49529962 | Msa0025910:CDS |
| ATATTCAGACCATTTGACAT+TGG | 0.548689 | 1_1:-49529948 | None:intergenic |
| AGTTGCGCAAAGGAGGTGAC+GGG | 0.556812 | 1_1:+49529454 | Msa0025910:CDS |
| AAGTTGCGCAAAGGAGGTGA+CGG | 0.559484 | 1_1:+49529453 | Msa0025910:CDS |
| TCTCTGCAGCAGCTCCGTCC+CGG | 0.570077 | 1_1:-49529497 | None:intergenic |
| GCTGCTGCAGAGATTGGAGA+TGG | 0.571294 | 1_1:+49529507 | Msa0025910:CDS |
| CTTCAAAAGTTGCGCAAAGG+AGG | 0.573380 | 1_1:+49529447 | Msa0025910:CDS |
| AACCGTTTCTTGCATGAGCG+AGG | 0.574230 | 1_1:-49530028 | None:intergenic |
| AGATCGGAGATGGGCCGGGA+CGG | 0.593534 | 1_1:+49529483 | Msa0025910:CDS |
| GGGGCTGCTAGATCGGAGAT+GGG | 0.600787 | 1_1:+49529474 | Msa0025910:CDS |
| TACCTTCAAAAGTTGCGCAA+AGG | 0.612577 | 1_1:+49529444 | Msa0025910:CDS |
| GTTGCGCAAAGGAGGTGACG+GGG | 0.703235 | 1_1:+49529455 | Msa0025910:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTCTAAAAAACAATTTTTG+TGG | - | chr1_1:49529618-49529637 | None:intergenic | 15.0% |
| !! | AGATAAATATGAAGTTAAAT+AGG | - | chr1_1:49530133-49530152 | None:intergenic | 15.0% |
| !!! | ATTCTAAAAAACAATTTTTG+TGG | - | chr1_1:49529618-49529637 | None:intergenic | 15.0% |
| !! | AGATAAATATGAAGTTAAAT+AGG | - | chr1_1:49530133-49530152 | None:intergenic | 15.0% |
| !!! | AAAAAACAATTTTTGTGGAT+GGG | - | chr1_1:49529613-49529632 | None:intergenic | 20.0% |
| !!! | TAAAAAACAATTTTTGTGGA+TGG | - | chr1_1:49529614-49529633 | None:intergenic | 20.0% |
| !!! | TAATATAATTGAAGCACTAA+TGG | + | chr1_1:49529708-49529727 | Msa0025910:intron | 20.0% |
| !! | CAATAACACATTTAAAGATA+AGG | - | chr1_1:49529904-49529923 | None:intergenic | 20.0% |
| !!! | AAAAAACAATTTTTGTGGAT+GGG | - | chr1_1:49529613-49529632 | None:intergenic | 20.0% |
| !!! | TAAAAAACAATTTTTGTGGA+TGG | - | chr1_1:49529614-49529633 | None:intergenic | 20.0% |
| !!! | TAATATAATTGAAGCACTAA+TGG | + | chr1_1:49529708-49529727 | Msa0025910:intron | 20.0% |
| !! | CAATAACACATTTAAAGATA+AGG | - | chr1_1:49529904-49529923 | None:intergenic | 20.0% |
| ! | TCGATAAAGATATCTACATT+TGG | - | chr1_1:49529666-49529685 | None:intergenic | 25.0% |
| ! | TGGCAAAATTAAATAAGAGA+AGG | + | chr1_1:49529728-49529747 | Msa0025910:intron | 25.0% |
| !! | GATTAATTACCTTTGAAAGA+AGG | - | chr1_1:49529832-49529851 | None:intergenic | 25.0% |
| !!! | ATAATGATGGAAACTTGTTA+TGG | - | chr1_1:49530206-49530225 | None:intergenic | 25.0% |
| ! | TAACAAGTTTCCATCATTAT+AGG | + | chr1_1:49530206-49530225 | Msa0025910:intron | 25.0% |
| ! | TCGATAAAGATATCTACATT+TGG | - | chr1_1:49529666-49529685 | None:intergenic | 25.0% |
| ! | TGGCAAAATTAAATAAGAGA+AGG | + | chr1_1:49529728-49529747 | Msa0025910:intron | 25.0% |
| !! | GATTAATTACCTTTGAAAGA+AGG | - | chr1_1:49529832-49529851 | None:intergenic | 25.0% |
| !!! | ATAATGATGGAAACTTGTTA+TGG | - | chr1_1:49530206-49530225 | None:intergenic | 25.0% |
| ! | TAACAAGTTTCCATCATTAT+AGG | + | chr1_1:49530206-49530225 | Msa0025910:intron | 25.0% |
| GTAATTGATAGATATGCTCT+TGG | + | chr1_1:49529750-49529769 | Msa0025910:intron | 30.0% | |
| CAGAGTTCAATCGTAATTAA+TGG | - | chr1_1:49529788-49529807 | None:intergenic | 30.0% | |
| ! | TGTGTTATTGAATTGTGTAG+TGG | + | chr1_1:49529914-49529933 | Msa0025910:intron | 30.0% |
| AAAAGATGTCCAATGTCAAA+TGG | + | chr1_1:49529939-49529958 | Msa0025910:CDS | 30.0% | |
| ATATTCAGACCATTTGACAT+TGG | - | chr1_1:49529951-49529970 | None:intergenic | 30.0% | |
| ACTATCTCCTTTGTTAATAG+GGG | - | chr1_1:49530084-49530103 | None:intergenic | 30.0% | |
| CACTATCTCCTTTGTTAATA+GGG | - | chr1_1:49530085-49530104 | None:intergenic | 30.0% | |
| !!! | AAAGGAGATAGTGGAATTTT+AGG | + | chr1_1:49530092-49530111 | Msa0025910:intron | 30.0% |
| AGTTTCCATCATTATAGGAA+TGG | + | chr1_1:49530211-49530230 | Msa0025910:intron | 30.0% | |
| GTAATTGATAGATATGCTCT+TGG | + | chr1_1:49529750-49529769 | Msa0025910:intron | 30.0% | |
| CAGAGTTCAATCGTAATTAA+TGG | - | chr1_1:49529788-49529807 | None:intergenic | 30.0% | |
| ! | TGTGTTATTGAATTGTGTAG+TGG | + | chr1_1:49529914-49529933 | Msa0025910:intron | 30.0% |
| AAAAGATGTCCAATGTCAAA+TGG | + | chr1_1:49529939-49529958 | Msa0025910:CDS | 30.0% | |
| ATATTCAGACCATTTGACAT+TGG | - | chr1_1:49529951-49529970 | None:intergenic | 30.0% | |
| ACTATCTCCTTTGTTAATAG+GGG | - | chr1_1:49530084-49530103 | None:intergenic | 30.0% | |
| CACTATCTCCTTTGTTAATA+GGG | - | chr1_1:49530085-49530104 | None:intergenic | 30.0% | |
| !!! | AAAGGAGATAGTGGAATTTT+AGG | + | chr1_1:49530092-49530111 | Msa0025910:intron | 30.0% |
| AGTTTCCATCATTATAGGAA+TGG | + | chr1_1:49530211-49530230 | Msa0025910:intron | 30.0% | |
| GTGCATGATCATAGTATACT+TGG | - | chr1_1:49529573-49529592 | None:intergenic | 35.0% | |
| ! | TATCGAGTTTGAAGCACTAA+TGG | + | chr1_1:49529680-49529699 | Msa0025910:intron | 35.0% |
| GGCAATTGTAGAAACAGTTA+GGG | - | chr1_1:49529811-49529830 | None:intergenic | 35.0% | |
| AGGCAATTGTAGAAACAGTT+AGG | - | chr1_1:49529812-49529831 | None:intergenic | 35.0% | |
| CTACAATTGCCTTCTTTCAA+AGG | + | chr1_1:49529820-49529839 | Msa0025910:intron | 35.0% | |
| TCTGAATATGTCGAAGTGAA+AGG | + | chr1_1:49529962-49529981 | Msa0025910:CDS | 35.0% | |
| CTGAATATGTCGAAGTGAAA+GGG | + | chr1_1:49529963-49529982 | Msa0025910:CDS | 35.0% | |
| !! | CGGTTGATGGTAAGAAAAAT+AGG | + | chr1_1:49530046-49530065 | Msa0025910:intron | 35.0% |
| CCACTATCTCCTTTGTTAAT+AGG | - | chr1_1:49530086-49530105 | None:intergenic | 35.0% | |
| CCTATTAACAAAGGAGATAG+TGG | + | chr1_1:49530083-49530102 | Msa0025910:intron | 35.0% | |
| GCTATTGAAGTCCTGATTAT+TGG | + | chr1_1:49530237-49530256 | Msa0025910:CDS | 35.0% | |
| GTGCATGATCATAGTATACT+TGG | - | chr1_1:49529573-49529592 | None:intergenic | 35.0% | |
| ! | TATCGAGTTTGAAGCACTAA+TGG | + | chr1_1:49529680-49529699 | Msa0025910:intron | 35.0% |
| GGCAATTGTAGAAACAGTTA+GGG | - | chr1_1:49529811-49529830 | None:intergenic | 35.0% | |
| AGGCAATTGTAGAAACAGTT+AGG | - | chr1_1:49529812-49529831 | None:intergenic | 35.0% | |
| CTACAATTGCCTTCTTTCAA+AGG | + | chr1_1:49529820-49529839 | Msa0025910:intron | 35.0% | |
| TCTGAATATGTCGAAGTGAA+AGG | + | chr1_1:49529962-49529981 | Msa0025910:CDS | 35.0% | |
| CTGAATATGTCGAAGTGAAA+GGG | + | chr1_1:49529963-49529982 | Msa0025910:CDS | 35.0% | |
| !! | CGGTTGATGGTAAGAAAAAT+AGG | + | chr1_1:49530046-49530065 | Msa0025910:intron | 35.0% |
| CCACTATCTCCTTTGTTAAT+AGG | - | chr1_1:49530086-49530105 | None:intergenic | 35.0% | |
| CCTATTAACAAAGGAGATAG+TGG | + | chr1_1:49530083-49530102 | Msa0025910:intron | 35.0% | |
| GCTATTGAAGTCCTGATTAT+TGG | + | chr1_1:49530237-49530256 | Msa0025910:CDS | 35.0% | |
| TACCTTCAAAAGTTGCGCAA+AGG | + | chr1_1:49529444-49529463 | Msa0025910:CDS | 40.0% | |
| ! | GATCGCGACATAACTGTTTT+TGG | + | chr1_1:49529529-49529548 | Msa0025910:CDS | 40.0% |
| TCTCCTTTGTTAATAGGGGT+GGG | - | chr1_1:49530080-49530099 | None:intergenic | 40.0% | |
| ATCTCCTTTGTTAATAGGGG+TGG | - | chr1_1:49530081-49530100 | None:intergenic | 40.0% | |
| GCATGCCATTCCTATAATGA+TGG | - | chr1_1:49530219-49530238 | None:intergenic | 40.0% | |
| TACCTTCAAAAGTTGCGCAA+AGG | + | chr1_1:49529444-49529463 | Msa0025910:CDS | 40.0% | |
| ! | GATCGCGACATAACTGTTTT+TGG | + | chr1_1:49529529-49529548 | Msa0025910:CDS | 40.0% |
| TCTCCTTTGTTAATAGGGGT+GGG | - | chr1_1:49530080-49530099 | None:intergenic | 40.0% | |
| ATCTCCTTTGTTAATAGGGG+TGG | - | chr1_1:49530081-49530100 | None:intergenic | 40.0% | |
| GCATGCCATTCCTATAATGA+TGG | - | chr1_1:49530219-49530238 | None:intergenic | 40.0% | |
| ! | CTCCTTTGCGCAACTTTTGA+AGG | - | chr1_1:49529449-49529468 | None:intergenic | 45.0% |
| CTTCAAAAGTTGCGCAAAGG+AGG | + | chr1_1:49529447-49529466 | Msa0025910:CDS | 45.0% | |
| TTCCTCGCTCATGCAAGAAA+CGG | + | chr1_1:49530026-49530045 | Msa0025910:CDS | 45.0% | |
| CTCATGCAAGAAACGGTTGA+TGG | + | chr1_1:49530033-49530052 | Msa0025910:CDS | 45.0% | |
| GTTCCCACCCCTATTAACAA+AGG | + | chr1_1:49530074-49530093 | Msa0025910:intron | 45.0% | |
| ! | CTCCTTTGCGCAACTTTTGA+AGG | - | chr1_1:49529449-49529468 | None:intergenic | 45.0% |
| CTTCAAAAGTTGCGCAAAGG+AGG | + | chr1_1:49529447-49529466 | Msa0025910:CDS | 45.0% | |
| TTCCTCGCTCATGCAAGAAA+CGG | + | chr1_1:49530026-49530045 | Msa0025910:CDS | 45.0% | |
| CTCATGCAAGAAACGGTTGA+TGG | + | chr1_1:49530033-49530052 | Msa0025910:CDS | 45.0% | |
| GTTCCCACCCCTATTAACAA+AGG | + | chr1_1:49530074-49530093 | Msa0025910:intron | 45.0% | |
| !! | TATCTAAAAATATAAAAAAT+AGG | + | chr1_1:49530148-49530167 | Msa0025910:intron | 5.0% |
| !! | TATCTAAAAATATAAAAAAT+AGG | + | chr1_1:49530148-49530167 | Msa0025910:intron | 5.0% |
| AAGTTGCGCAAAGGAGGTGA+CGG | + | chr1_1:49529453-49529472 | Msa0025910:CDS | 50.0% | |
| AACCGTTTCTTGCATGAGCG+AGG | - | chr1_1:49530031-49530050 | None:intergenic | 50.0% | |
| AAGTTGCGCAAAGGAGGTGA+CGG | + | chr1_1:49529453-49529472 | Msa0025910:CDS | 50.0% | |
| AACCGTTTCTTGCATGAGCG+AGG | - | chr1_1:49530031-49530050 | None:intergenic | 50.0% | |
| AGTTGCGCAAAGGAGGTGAC+GGG | + | chr1_1:49529454-49529473 | Msa0025910:CDS | 55.0% | |
| GCTGCTGCAGAGATTGGAGA+TGG | + | chr1_1:49529507-49529526 | Msa0025910:CDS | 55.0% | |
| AGTTGCGCAAAGGAGGTGAC+GGG | + | chr1_1:49529454-49529473 | Msa0025910:CDS | 55.0% | |
| GCTGCTGCAGAGATTGGAGA+TGG | + | chr1_1:49529507-49529526 | Msa0025910:CDS | 55.0% | |
| GTTGCGCAAAGGAGGTGACG+GGG | + | chr1_1:49529455-49529474 | Msa0025910:CDS | 60.0% | |
| !! | AGGTGACGGGGCTGCTAGAT+CGG | + | chr1_1:49529467-49529486 | Msa0025910:CDS | 60.0% |
| GGGGCTGCTAGATCGGAGAT+GGG | + | chr1_1:49529474-49529493 | Msa0025910:CDS | 60.0% | |
| CTGCTAGATCGGAGATGGGC+CGG | + | chr1_1:49529478-49529497 | Msa0025910:CDS | 60.0% | |
| TGCTAGATCGGAGATGGGCC+GGG | + | chr1_1:49529479-49529498 | Msa0025910:CDS | 60.0% | |
| GACGGAGCTGCTGCAGAGAT+TGG | + | chr1_1:49529501-49529520 | Msa0025910:CDS | 60.0% | |
| GTTGCGCAAAGGAGGTGACG+GGG | + | chr1_1:49529455-49529474 | Msa0025910:CDS | 60.0% | |
| !! | AGGTGACGGGGCTGCTAGAT+CGG | + | chr1_1:49529467-49529486 | Msa0025910:CDS | 60.0% |
| GGGGCTGCTAGATCGGAGAT+GGG | + | chr1_1:49529474-49529493 | Msa0025910:CDS | 60.0% | |
| CTGCTAGATCGGAGATGGGC+CGG | + | chr1_1:49529478-49529497 | Msa0025910:CDS | 60.0% | |
| TGCTAGATCGGAGATGGGCC+GGG | + | chr1_1:49529479-49529498 | Msa0025910:CDS | 60.0% | |
| GACGGAGCTGCTGCAGAGAT+TGG | + | chr1_1:49529501-49529520 | Msa0025910:CDS | 60.0% | |
| CGGGGCTGCTAGATCGGAGA+TGG | + | chr1_1:49529473-49529492 | Msa0025910:CDS | 65.0% | |
| AGATCGGAGATGGGCCGGGA+CGG | + | chr1_1:49529483-49529502 | Msa0025910:CDS | 65.0% | |
| TCTCTGCAGCAGCTCCGTCC+CGG | - | chr1_1:49529500-49529519 | None:intergenic | 65.0% | |
| CGGGGCTGCTAGATCGGAGA+TGG | + | chr1_1:49529473-49529492 | Msa0025910:CDS | 65.0% | |
| AGATCGGAGATGGGCCGGGA+CGG | + | chr1_1:49529483-49529502 | Msa0025910:CDS | 65.0% | |
| TCTCTGCAGCAGCTCCGTCC+CGG | - | chr1_1:49529500-49529519 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_1 | gene | 49529439 | 49530266 | 49529439 | ID=Msa0025910;Name=Msa0025910 |
| chr1_1 | mRNA | 49529439 | 49530266 | 49529439 | ID=Msa0025910-mRNA-1;Parent=Msa0025910;Name=Msa0025910-mRNA-1;_AED=0.63;_eAED=0.83;_QI=0|0|0.33|0.66|0|0.33|3|0|120 |
| chr1_1 | exon | 49529439 | 49529641 | 49529439 | ID=Msa0025910-mRNA-1:exon:14265;Parent=Msa0025910-mRNA-1 |
| chr1_1 | exon | 49529934 | 49530054 | 49529934 | ID=Msa0025910-mRNA-1:exon:14266;Parent=Msa0025910-mRNA-1 |
| chr1_1 | exon | 49530228 | 49530266 | 49530228 | ID=Msa0025910-mRNA-1:exon:14267;Parent=Msa0025910-mRNA-1 |
| chr1_1 | CDS | 49529439 | 49529641 | 49529439 | ID=Msa0025910-mRNA-1:cds;Parent=Msa0025910-mRNA-1 |
| chr1_1 | CDS | 49529934 | 49530054 | 49529934 | ID=Msa0025910-mRNA-1:cds;Parent=Msa0025910-mRNA-1 |
| chr1_1 | CDS | 49530228 | 49530266 | 49530228 | ID=Msa0025910-mRNA-1:cds;Parent=Msa0025910-mRNA-1 |
| Gene Sequence |
| Protein sequence |
