AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0034170

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 61 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTTTGATTTCTCCAAATT+TGG 0.124200 1_1:-62047646 Msa0034170:three_prime_UTR
AACACTTTCTTCTTGATTAA+TGG 0.164042 1_1:+62047044 None:intergenic
TTTCGTTACTACTTTGTTTC+AGG 0.182375 1_1:+62047284 None:intergenic
TGATGCTAACTTACCAAATT+TGG 0.190980 1_1:+62047633 None:intergenic
AACATTTAAAGAGTGGAAAT+TGG 0.288206 1_1:-62047532 Msa0034170:three_prime_UTR
CCAGAAGAAGGCTGCAATCT+TGG 0.293966 1_1:-62049466 Msa0034170:intron
TGGTTACATATCAGTAAATA+TGG 0.310000 1_1:-62047732 Msa0034170:exon
AAGTGAAGATGAGAGTAAAA+AGG 0.323034 1_1:-62049813 Msa0034170:five_prime_UTR
GAGAAAGTGAAATGAGAAAA+GGG 0.354240 1_1:-62047123 Msa0034170:three_prime_UTR
ATCTCATGTTCTTTCGCCAT+TGG 0.365295 1_1:+62049783 None:intergenic
AGCATACATTTAGAACAATT+AGG 0.372108 1_1:+62047462 None:intergenic
CAGAAGAAGGCTGCAATCTT+GGG 0.389347 1_1:-62049465 Msa0034170:CDS
TGAGAAAGTGAAATGAGAAA+AGG 0.410271 1_1:-62047124 Msa0034170:three_prime_UTR
TCGGCCACTGCTTGCTCCCA+TGG 0.444855 1_1:-62049716 Msa0034170:exon
GCTTTCACAGTCCTGCTGCC+TGG 0.448612 1_1:+62047185 None:intergenic
CTAATCTTCATATCCTGTAT+TGG 0.450990 1_1:+62049758 None:intergenic
TGAGAAAAGGGGAAGGTTTG+TGG 0.479827 1_1:-62047111 Msa0034170:three_prime_UTR
TTGGAGAAACATTTAAAGAG+TGG 0.488211 1_1:-62047539 Msa0034170:three_prime_UTR
TGCAGGCACACAAAAGTGGC+AGG 0.491564 1_1:-62049197 Msa0034170:intron
AAATTAATGTAAAAGTTGTA+AGG 0.519799 1_1:+62047565 None:intergenic
GAACATGAGATGACCAATAC+AGG 0.521790 1_1:-62049771 Msa0034170:five_prime_UTR
TTCTCATTTCACTTTCTCAA+AGG 0.523484 1_1:+62047128 None:intergenic
GATTGTAGCTTCTTGAGGAA+TGG 0.535247 1_1:-62047307 Msa0034170:three_prime_UTR
AGCAAGCAGTGGCCGAGTGT+CGG 0.545578 1_1:+62049723 None:intergenic
TCTCAGTCATAAAAGACATA+CGG 0.549138 1_1:+62050063 None:intergenic
AATTCCATAGTTGTCGCAAA+GGG 0.552910 1_1:-62047785 Msa0034170:intron
TCAAGAAGCTACAATCCCTC+TGG 0.555731 1_1:+62047314 None:intergenic
CGTTTCAATTCAATGACCAT+GGG 0.564107 1_1:+62049700 None:intergenic
TCGTTTCAATTCAATGACCA+TGG 0.564723 1_1:+62049699 None:intergenic
AGAGGGATTGTAGCTTCTTG+AGG 0.568840 1_1:-62047312 Msa0034170:three_prime_UTR
AGAAAGTGAAATGAGAAAAG+GGG 0.570896 1_1:-62047122 Msa0034170:three_prime_UTR
CGACGAAGAGGAAAGGAAGA+TGG 0.574038 1_1:-62047752 Msa0034170:CDS
TAGTTGTCGCAAAGGGTGAA+AGG 0.577451 1_1:-62047778 Msa0034170:CDS
CGTATGCAGAAGTCCGCTTG+TGG 0.584859 1_1:+62049992 None:intergenic
TAATTCCATAGTTGTCGCAA+AGG 0.591523 1_1:-62047786 Msa0034170:intron
TACAGGATATGAAGATTAGA+CGG 0.591621 1_1:-62049754 Msa0034170:five_prime_UTR
CACAAGCGGACTTCTGCATA+CGG 0.594326 1_1:-62049991 Msa0034170:intron
AAGGAAACGACGAAGAGGAA+AGG 0.601511 1_1:-62047759 Msa0034170:CDS
CAGGCACACAAAAGTGGCAG+GGG 0.602763 1_1:-62049195 Msa0034170:CDS
GCAGGCACACAAAAGTGGCA+GGG 0.607604 1_1:-62049196 Msa0034170:intron
GTTCCAACACCGACTCTATG+TGG 0.613390 1_1:+62047163 None:intergenic
ATATGAAGAAATAATATCAA+CGG 0.618034 1_1:-62047261 Msa0034170:three_prime_UTR
ACATTGCAGGCACACAAAAG+TGG 0.622491 1_1:-62049201 Msa0034170:intron
CTCATCAGAGAAGCCACAAG+CGG 0.623400 1_1:-62050005 Msa0034170:five_prime_UTR
AGAGAAATAGGACGAAAGAG+AGG 0.624933 1_1:-62050043 Msa0034170:five_prime_UTR
TAGAGTCGGTGTTGGAACGT+TGG 0.626662 1_1:-62047158 Msa0034170:three_prime_UTR
AAGCCACATAGAGTCGGTGT+TGG 0.626714 1_1:-62047166 Msa0034170:three_prime_UTR
TCTCTGTGTATCTTAGCCGA+TGG 0.628089 1_1:+62047490 None:intergenic
GGTTTGTGGTCCATCACCAT+CGG 0.630906 1_1:-62047097 Msa0034170:three_prime_UTR
AGTGAAATGAGAAAAGGGGA+AGG 0.632610 1_1:-62047118 Msa0034170:three_prime_UTR
AGCGATATATAGATACCAGA+GGG 0.635782 1_1:-62047329 Msa0034170:three_prime_UTR
AGAACTGTGAAATTTAAGCA+AGG 0.639752 1_1:-62049843 Msa0034170:five_prime_UTR
GGTGAAAGGAAACGACGAAG+AGG 0.640858 1_1:-62047764 Msa0034170:CDS
AAATGTGCAGTAGCTGCCAT+CGG 0.645054 1_1:-62047506 Msa0034170:three_prime_UTR
ATGACCATGGGAGCAAGCAG+TGG 0.647526 1_1:+62049712 None:intergenic
TCTTTACTTTGATAAACACA+AGG 0.651085 1_1:-62049153 Msa0034170:intron
GAGCGATATATAGATACCAG+AGG 0.665812 1_1:-62047330 Msa0034170:three_prime_UTR
CTGTGAAAGCCACATAGAGT+CGG 0.666824 1_1:-62047172 Msa0034170:three_prime_UTR
TTAGCATCACTGTTGCAACA+TGG 0.698789 1_1:-62047619 Msa0034170:three_prime_UTR
ATGGTCATTGAATTGAAACG+AGG 0.731445 1_1:-62049697 Msa0034170:CDS
ATCGGCTAAGATACACAGAG+AGG 0.735944 1_1:-62047488 Msa0034170:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTACAAAAAATTTCTTT+TGG + chr1_1:62048202-62048221 None:intergenic 10.0%
!!! AATTTTTTGTAATTAATCAT+TGG - chr1_1:62048207-62048226 Msa0034170:intron 10.0%
!! AAAAAAATTAGTAAGGAAAT+TGG + chr1_1:62047358-62047377 None:intergenic 15.0%
!! GTTTACAAAACATATTAATA+TGG + chr1_1:62048037-62048056 None:intergenic 15.0%
!! AGATAAAAATAAGAAACAAT+AGG + chr1_1:62048437-62048456 None:intergenic 15.0%
!! TAAATTGAAGGAAATTTAAA+AGG + chr1_1:62048709-62048728 None:intergenic 15.0%
!! TGTTTAAGTAAATATAGTAA+TGG + chr1_1:62048732-62048751 None:intergenic 15.0%
!! TAATACAAATTCAGATATAA+TGG - chr1_1:62048924-62048943 Msa0034170:intron 15.0%
!! AATACAAATTCAGATATAAT+GGG - chr1_1:62048925-62048944 Msa0034170:intron 15.0%
!! AAATTAATGTAAAAGTTGTA+AGG + chr1_1:62049295-62049314 None:intergenic 15.0%
!!! TACAACTTTTACATTAATTT+TGG - chr1_1:62049295-62049314 Msa0034170:intron 15.0%
!! ATATGAAGAAATAATATCAA+CGG - chr1_1:62049596-62049615 Msa0034170:intron 15.0%
!! TAAAGGGAAAAAAATTAGTA+AGG + chr1_1:62047365-62047384 None:intergenic 20.0%
!! AGGGTATATAACATATATAA+AGG - chr1_1:62047578-62047597 Msa0034170:three_prime_UTR 20.0%
!!! ATCATTTTTATGCCAAAATT+AGG + chr1_1:62047611-62047630 None:intergenic 20.0%
!! TGTGGAAATAATAAAATTAG+TGG + chr1_1:62047639-62047658 None:intergenic 20.0%
!!! TTCTTTCTTCATGTTTTTAT+TGG + chr1_1:62048550-62048569 None:intergenic 20.0%
!! TAATAGAATAACCTTAGTTT+GGG + chr1_1:62048615-62048634 None:intergenic 20.0%
!! ATATAGTAATGGTAAATTGA+AGG + chr1_1:62048721-62048740 None:intergenic 20.0%
!! AGATAATATGGAGAAAAAAT+TGG + chr1_1:62048999-62049018 None:intergenic 20.0%
!!! ACTTTTACATTAATTTTGGT+TGG - chr1_1:62049299-62049318 Msa0034170:intron 20.0%
!! ACAAAAAAACAAAAAGTTGA+TGG + chr1_1:62049886-62049905 None:intergenic 20.0%
!! TTTTACAATACAGACAGTTA+AGG - chr1_1:62046990-62047009 Msa0034170:three_prime_UTR 25.0%
!! ATTTTTATGCCAAAATTAGG+TGG + chr1_1:62047608-62047627 None:intergenic 25.0%
!! TTTTATTATTTCCACATTGC+AGG - chr1_1:62047643-62047662 Msa0034170:three_prime_UTR 25.0%
! TCTTTACTTTGATAAACACA+AGG - chr1_1:62047704-62047723 Msa0034170:three_prime_UTR 25.0%
! TAAACACAAGGTAAATATAC+TGG - chr1_1:62047716-62047735 Msa0034170:three_prime_UTR 25.0%
! GAAAGATAGATAGAGAAAAA+AGG - chr1_1:62047872-62047891 Msa0034170:intron 25.0%
!! TCATCTTCAAATTTTTCCTT+TGG + chr1_1:62047910-62047929 None:intergenic 25.0%
! AAAAGGTCAGTTTGAATAAT+TGG + chr1_1:62047951-62047970 None:intergenic 25.0%
! TTTGGATGAGAATATGAATT+TGG - chr1_1:62047979-62047998 Msa0034170:intron 25.0%
!! GTGTTTAAAATTTGAGTAGA+AGG + chr1_1:62048072-62048091 None:intergenic 25.0%
! TTGTAAATCGGAATTTGTAA+AGG + chr1_1:62048172-62048191 None:intergenic 25.0%
!!! TTTGGTTTGTGATTGTAAAT+CGG + chr1_1:62048184-62048203 None:intergenic 25.0%
! TTAAACACTTTAATTAGTGG+TGG + chr1_1:62048395-62048414 None:intergenic 25.0%
!! ACCACTAATTAAAGTGTTTA+AGG - chr1_1:62048394-62048413 Msa0034170:intron 25.0%
! ACCTTAAACACTTTAATTAG+TGG + chr1_1:62048398-62048417 None:intergenic 25.0%
! CATCTATACACAAAATTAAC+AGG + chr1_1:62048515-62048534 None:intergenic 25.0%
!! CTGTTAATTTTGTGTATAGA+TGG - chr1_1:62048513-62048532 Msa0034170:intron 25.0%
! AAACATGAAGAAAGAAGTTT+AGG - chr1_1:62048554-62048573 Msa0034170:intron 25.0%
! AGAAGTTTAGGAAATTTGAA+AGG - chr1_1:62048566-62048585 Msa0034170:intron 25.0%
!! AATAGAATAACCTTAGTTTG+GGG + chr1_1:62048614-62048633 None:intergenic 25.0%
! CTAATAGAATAACCTTAGTT+TGG + chr1_1:62048616-62048635 None:intergenic 25.0%
! TAAGGTTATTCTATTAGCAA+GGG - chr1_1:62048619-62048638 Msa0034170:intron 25.0%
! AGAAATTAACACAACAAAGA+AGG + chr1_1:62048798-62048817 None:intergenic 25.0%
! TATTTCCTATTTACTCTTCT+TGG - chr1_1:62048900-62048919 Msa0034170:intron 25.0%
! ATTAACCAAGAAGAGTAAAT+AGG + chr1_1:62048908-62048927 None:intergenic 25.0%
!! TTTTTCTCCATATTATCTCT+CGG - chr1_1:62049001-62049020 Msa0034170:intron 25.0%
! TGGTTACATATCAGTAAATA+TGG - chr1_1:62049125-62049144 Msa0034170:intron 25.0%
!! TTCTTTGATTTCTCCAAATT+TGG - chr1_1:62049211-62049230 Msa0034170:intron 25.0%
! AACATTTAAAGAGTGGAAAT+TGG - chr1_1:62049325-62049344 Msa0034170:intron 25.0%
!! AGCATACATTTAGAACAATT+AGG + chr1_1:62049398-62049417 None:intergenic 25.0%
! AAGAATTCAATTGTACCAAA+AGG - chr1_1:62049420-62049439 Msa0034170:intron 25.0%
! AGAATTCAATTGTACCAAAA+GGG - chr1_1:62049421-62049440 Msa0034170:intron 25.0%
!! AAAAGAAGAAAAAACCCTTT+TGG + chr1_1:62049438-62049457 None:intergenic 25.0%
!!! TCACAACAATGTTTTTGTAA+TGG - chr1_1:62049623-62049642 Msa0034170:intron 25.0%
!!! AGATTGTGATATTTTTATCC+AGG - chr1_1:62049654-62049673 Msa0034170:intron 25.0%
! AACACTTTCTTCTTGATTAA+TGG + chr1_1:62049816-62049835 None:intergenic 25.0%
!!! TGTTTTTTTGTTTGCTTTTG+TGG - chr1_1:62049895-62049914 Msa0034170:intron 25.0%
TCTCAGTCATAAAAGACATA+CGG + chr1_1:62046797-62046816 None:intergenic 30.0%
! CTTTTATGACTGAGAGAAAT+AGG - chr1_1:62046802-62046821 Msa0034170:three_prime_UTR 30.0%
AGAACTGTGAAATTTAAGCA+AGG - chr1_1:62047014-62047033 Msa0034170:three_prime_UTR 30.0%
AAGTGAAGATGAGAGTAAAA+AGG - chr1_1:62047044-62047063 Msa0034170:three_prime_UTR 30.0%
CTAATCTTCATATCCTGTAT+TGG + chr1_1:62047102-62047121 None:intergenic 30.0%
TACAGGATATGAAGATTAGA+CGG - chr1_1:62047103-62047122 Msa0034170:three_prime_UTR 30.0%
!! TGTTCGTGTTTTTATAAGTG+AGG - chr1_1:62047431-62047450 Msa0034170:intron 30.0%
!! GTTCGTGTTTTTATAAGTGA+GGG - chr1_1:62047432-62047451 Msa0034170:intron 30.0%
ACACAAACAATACTTTGCAT+AGG + chr1_1:62047465-62047484 None:intergenic 30.0%
TTGATTACTAAATGCAGTGA+GGG - chr1_1:62047559-62047578 Msa0034170:three_prime_UTR 30.0%
! AGGCTTGGTTAATCTAATTT+AGG - chr1_1:62047757-62047776 Msa0034170:CDS 30.0%
TTTCTCTATCTATCTTTCAC+TGG + chr1_1:62047870-62047889 None:intergenic 30.0%
! AAATTTTTCCTTTGGTATGC+AGG + chr1_1:62047902-62047921 None:intergenic 30.0%
TCATCCAAATCAATAGCAAA+AGG + chr1_1:62047968-62047987 None:intergenic 30.0%
TTTGTGCTTTGTAATTTCGT+TGG - chr1_1:62048286-62048305 Msa0034170:intron 30.0%
TTGTGCTTTGTAATTTCGTT+GGG - chr1_1:62048287-62048306 Msa0034170:intron 30.0%
CTATACACAAAATTAACAGG+TGG + chr1_1:62048512-62048531 None:intergenic 30.0%
CTAAGGTTATTCTATTAGCA+AGG - chr1_1:62048618-62048637 Msa0034170:intron 30.0%
AAGGTTATTCTATTAGCAAG+GGG - chr1_1:62048620-62048639 Msa0034170:intron 30.0%
GAGAAACTCCTCATATATAT+AGG - chr1_1:62048966-62048985 Msa0034170:intron 30.0%
AACTCCTCATATATATAGGA+AGG - chr1_1:62048970-62048989 Msa0034170:intron 30.0%
TGATGCTAACTTACCAAATT+TGG + chr1_1:62049227-62049246 None:intergenic 30.0%
TTGGAGAAACATTTAAAGAG+TGG - chr1_1:62049318-62049337 Msa0034170:intron 30.0%
! TTTCGTTACTACTTTGTTTC+AGG + chr1_1:62049576-62049595 None:intergenic 30.0%
TTCTCATTTCACTTTCTCAA+AGG + chr1_1:62049732-62049751 None:intergenic 30.0%
TGAGAAAGTGAAATGAGAAA+AGG - chr1_1:62049733-62049752 Msa0034170:five_prime_UTR 30.0%
GAGAAAGTGAAATGAGAAAA+GGG - chr1_1:62049734-62049753 Msa0034170:five_prime_UTR 30.0%
AGAAAGTGAAATGAGAAAAG+GGG - chr1_1:62049735-62049754 Msa0034170:five_prime_UTR 30.0%
AATTCCACAGAATCATGAAG+AGG + chr1_1:62046943-62046962 None:intergenic 35.0%
GTAAAAAGGAGTAAGACCAA+TGG - chr1_1:62047058-62047077 Msa0034170:three_prime_UTR 35.0%
CGTTTCAATTCAATGACCAT+GGG + chr1_1:62047160-62047179 None:intergenic 35.0%
TCGTTTCAATTCAATGACCA+TGG + chr1_1:62047161-62047180 None:intergenic 35.0%
ATGGTCATTGAATTGAAACG+AGG - chr1_1:62047160-62047179 Msa0034170:three_prime_UTR 35.0%
! CTTCTTCTGGAGAGTATAAA+GGG + chr1_1:62047381-62047400 None:intergenic 35.0%
CTTGATTACTAAATGCAGTG+AGG - chr1_1:62047558-62047577 Msa0034170:three_prime_UTR 35.0%
! AAAGGCAAACCACCTAATTT+TGG - chr1_1:62047596-62047615 Msa0034170:three_prime_UTR 35.0%
GGTTTGATTACTTTCTTTCG+AGG - chr1_1:62047737-62047756 Msa0034170:exon 35.0%
TTGAAAAGCGATGTTTGACT+AGG - chr1_1:62047795-62047814 Msa0034170:intron 35.0%
! TCTTTCACTGGTTGAATCTA+TGG + chr1_1:62047858-62047877 None:intergenic 35.0%
!!! CTGACCTTTTGCTATTGATT+TGG - chr1_1:62047961-62047980 Msa0034170:intron 35.0%
CACATCCATGTTCTTTCAAT+TGG - chr1_1:62048089-62048108 Msa0034170:intron 35.0%
CCTATCCAATTGAAAGAACA+TGG + chr1_1:62048097-62048116 None:intergenic 35.0%
CCATGTTCTTTCAATTGGAT+AGG - chr1_1:62048094-62048113 Msa0034170:intron 35.0%
GTTCTTTCAATTGGATAGGA+AGG - chr1_1:62048098-62048117 Msa0034170:intron 35.0%
TGTGCTTTGTAATTTCGTTG+GGG - chr1_1:62048288-62048307 Msa0034170:intron 35.0%
TTCATCTCTTTGCTCTCAAT+AGG + chr1_1:62048319-62048338 None:intergenic 35.0%
! AATTAGTGGTGGAGGAAAAT+AGG + chr1_1:62048384-62048403 None:intergenic 35.0%
AACACTTTAATTAGTGGTGG+AGG + chr1_1:62048392-62048411 None:intergenic 35.0%
TACACAAAATTAACAGGTGG+TGG + chr1_1:62048509-62048528 None:intergenic 35.0%
TGTTAATTTCTGTTCCACAG+AGG - chr1_1:62048807-62048826 Msa0034170:intron 35.0%
GAGGAGTTTCTCTATTAATG+CGG + chr1_1:62048958-62048977 None:intergenic 35.0%
GCAACCTTCCTATATATATG+AGG + chr1_1:62048977-62048996 None:intergenic 35.0%
TAAGAGACCGAGAGATAATA+TGG + chr1_1:62049011-62049030 None:intergenic 35.0%
TTTGCGACAACTATGGAATT+AGG + chr1_1:62049072-62049091 None:intergenic 35.0%
TAATTCCATAGTTGTCGCAA+AGG - chr1_1:62049071-62049090 Msa0034170:intron 35.0%
AATTCCATAGTTGTCGCAAA+GGG - chr1_1:62049072-62049091 Msa0034170:intron 35.0%
AGCGATATATAGATACCAGA+GGG - chr1_1:62049528-62049547 Msa0034170:intron 35.0%
!!! TGTTTTATGTGCTCTGAAGT+GGG - chr1_1:62049924-62049943 Msa0034170:intron 35.0%
AGAGAAATAGGACGAAAGAG+AGG - chr1_1:62046814-62046833 Msa0034170:three_prime_UTR 40.0%
CACAGAATCATGAAGAGGAA+GGG + chr1_1:62046938-62046957 None:intergenic 40.0%
ATCTCATGTTCTTTCGCCAT+TGG + chr1_1:62047077-62047096 None:intergenic 40.0%
GAACATGAGATGACCAATAC+AGG - chr1_1:62047086-62047105 Msa0034170:three_prime_UTR 40.0%
! CCTTCTTCTGGAGAGTATAA+AGG + chr1_1:62047382-62047401 None:intergenic 40.0%
CCTTTATACTCTCCAGAAGA+AGG - chr1_1:62047379-62047398 Msa0034170:intron 40.0%
GATTACTTTCTTTCGAGGCT+TGG - chr1_1:62047742-62047761 Msa0034170:CDS 40.0%
AGCGATGTTTGACTAGGAAA+AGG - chr1_1:62047801-62047820 Msa0034170:intron 40.0%
AAGGTTTACCTGCATACCAA+AGG - chr1_1:62047891-62047910 Msa0034170:intron 40.0%
GAGTAGAAGGCTTCATAACA+TGG + chr1_1:62048059-62048078 None:intergenic 40.0%
TGAGAGCAAAGAGATGAAGA+AGG - chr1_1:62048321-62048340 Msa0034170:intron 40.0%
GAGAGCAAAGAGATGAAGAA+GGG - chr1_1:62048322-62048341 Msa0034170:intron 40.0%
AAGAGATGAAGAAGGGCAAA+AGG - chr1_1:62048329-62048348 Msa0034170:intron 40.0%
AGAGATGAAGAAGGGCAAAA+GGG - chr1_1:62048330-62048349 Msa0034170:intron 40.0%
! ACAAAATTAACAGGTGGTGG+AGG + chr1_1:62048506-62048525 None:intergenic 40.0%
GCTTCTTGAAGCGATTGTTT+AGG + chr1_1:62048654-62048673 None:intergenic 40.0%
ATTCGTTCCGTTAGAAGAAG+TGG + chr1_1:62048836-62048855 None:intergenic 40.0%
TTAGCATCACTGTTGCAACA+TGG - chr1_1:62049238-62049257 Msa0034170:intron 40.0%
GAGCGATATATAGATACCAG+AGG - chr1_1:62049527-62049546 Msa0034170:intron 40.0%
GATTGTAGCTTCTTGAGGAA+TGG - chr1_1:62049550-62049569 Msa0034170:intron 40.0%
!! GATATTTTTATCCAGGCAGC+AGG - chr1_1:62049661-62049680 Msa0034170:intron 40.0%
AGTGAAATGAGAAAAGGGGA+AGG - chr1_1:62049739-62049758 Msa0034170:five_prime_UTR 40.0%
!!! GTGTTTTATGTGCTCTGAAG+TGG - chr1_1:62049923-62049942 Msa0034170:intron 40.0%
CCACAGAATCATGAAGAGGA+AGG + chr1_1:62046939-62046958 None:intergenic 45.0%
CCTTCCTCTTCATGATTCTG+TGG - chr1_1:62046936-62046955 Msa0034170:three_prime_UTR 45.0%
!!! ACGGTTTTTTGTCCGACACT+CGG - chr1_1:62047122-62047141 Msa0034170:three_prime_UTR 45.0%
CAGAAGAAGGCTGCAATCTT+GGG - chr1_1:62047392-62047411 Msa0034170:intron 45.0%
! ACTTTTGTGTGCCTGCAATG+TGG + chr1_1:62047657-62047676 None:intergenic 45.0%
ACATTGCAGGCACACAAAAG+TGG - chr1_1:62047656-62047675 Msa0034170:three_prime_UTR 45.0%
GCAGTACAGTGATGAGGAAA+AGG + chr1_1:62048592-62048611 None:intergenic 45.0%
CGACGCATAACTTTCTGCAT+AGG + chr1_1:62048676-62048695 None:intergenic 45.0%
TGCAGAAAGTTATGCGTCGT+TGG - chr1_1:62048677-62048696 Msa0034170:intron 45.0%
GAGGAAGCCACTTCTTCTAA+CGG - chr1_1:62048826-62048845 Msa0034170:intron 45.0%
TTCACCCTTTGCGACAACTA+TGG + chr1_1:62049079-62049098 None:intergenic 45.0%
TAGTTGTCGCAAAGGGTGAA+AGG - chr1_1:62049079-62049098 Msa0034170:intron 45.0%
AAGGAAACGACGAAGAGGAA+AGG - chr1_1:62049098-62049117 Msa0034170:intron 45.0%
AAATGTGCAGTAGCTGCCAT+CGG - chr1_1:62049351-62049370 Msa0034170:intron 45.0%
TCTCTGTGTATCTTAGCCGA+TGG + chr1_1:62049370-62049389 None:intergenic 45.0%
ATCGGCTAAGATACACAGAG+AGG - chr1_1:62049369-62049388 Msa0034170:intron 45.0%
TCAAGAAGCTACAATCCCTC+TGG + chr1_1:62049546-62049565 None:intergenic 45.0%
AGAGGGATTGTAGCTTCTTG+AGG - chr1_1:62049545-62049564 Msa0034170:intron 45.0%
CTGTGAAAGCCACATAGAGT+CGG - chr1_1:62049685-62049704 Msa0034170:intron 45.0%
TGAGAAAAGGGGAAGGTTTG+TGG - chr1_1:62049746-62049765 Msa0034170:five_prime_UTR 45.0%
! ATACTTTTCGCCGATGGTGA+TGG + chr1_1:62049773-62049792 None:intergenic 45.0%
! ACAGTGATACTTTTCGCCGA+TGG + chr1_1:62049779-62049798 None:intergenic 45.0%
!! AAAAAAAAAATATACATAAA+TGG + chr1_1:62046973-62046992 None:intergenic 5.0%
!!! ATTTTATAAAATTCAATAAT+CGG + chr1_1:62047272-62047291 None:intergenic 5.0%
!! AAATGAAAAAAATAATAAAT+AGG - chr1_1:62049970-62049989 Msa0034170:intron 5.0%
CTCATCAGAGAAGCCACAAG+CGG - chr1_1:62046852-62046871 Msa0034170:three_prime_UTR 50.0%
! CACAAGCGGACTTCTGCATA+CGG - chr1_1:62046866-62046885 Msa0034170:three_prime_UTR 50.0%
CCAAGATTGCAGCCTTCTTC+TGG + chr1_1:62047394-62047413 None:intergenic 50.0%
CCAGAAGAAGGCTGCAATCT+TGG - chr1_1:62047391-62047410 Msa0034170:intron 50.0%
GATGAAGAAGGGCAAAAGGG+AGG - chr1_1:62048333-62048352 Msa0034170:intron 50.0%
GGCAAAAGGGAGGAAGAAGA+TGG - chr1_1:62048343-62048362 Msa0034170:intron 50.0%
! TTTGGGGCAGTACAGTGATG+AGG + chr1_1:62048598-62048617 None:intergenic 50.0%
CACTGTACTGCCCCAAACTA+AGG - chr1_1:62048601-62048620 Msa0034170:intron 50.0%
AGAAGAAGTGGCTTCCTCTG+TGG + chr1_1:62048824-62048843 None:intergenic 50.0%
GGTGAAAGGAAACGACGAAG+AGG - chr1_1:62049093-62049112 Msa0034170:intron 50.0%
CGACGAAGAGGAAAGGAAGA+TGG - chr1_1:62049105-62049124 Msa0034170:intron 50.0%
AAGCCACATAGAGTCGGTGT+TGG - chr1_1:62049691-62049710 Msa0034170:CDS 50.0%
!! GTTCCAACACCGACTCTATG+TGG + chr1_1:62049697-62049716 None:intergenic 50.0%
!! TAGAGTCGGTGTTGGAACGT+TGG - chr1_1:62049699-62049718 Msa0034170:CDS 50.0%
GGTTTGTGGTCCATCACCAT+CGG - chr1_1:62049760-62049779 Msa0034170:five_prime_UTR 50.0%
CGTATGCAGAAGTCCGCTTG+TGG + chr1_1:62046868-62046887 None:intergenic 55.0%
ATGACCATGGGAGCAAGCAG+TGG + chr1_1:62047148-62047167 None:intergenic 55.0%
TGCAGGCACACAAAAGTGGC+AGG - chr1_1:62047660-62047679 Msa0034170:three_prime_UTR 55.0%
GCAGGCACACAAAAGTGGCA+GGG - chr1_1:62047661-62047680 Msa0034170:three_prime_UTR 55.0%
CAGGCACACAAAAGTGGCAG+GGG - chr1_1:62047662-62047681 Msa0034170:three_prime_UTR 55.0%
AGCAAGCAGTGGCCGAGTGT+CGG + chr1_1:62047137-62047156 None:intergenic 60.0%
GCTTTCACAGTCCTGCTGCC+TGG + chr1_1:62049675-62049694 None:intergenic 60.0%
TCGGCCACTGCTTGCTCCCA+TGG - chr1_1:62047141-62047160 Msa0034170:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 62046789 62050090 62046789 ID=Msa0034170;Name=Msa0034170
chr1_1 mRNA 62046789 62050090 62046789 ID=Msa0034170-mRNA-1;Parent=Msa0034170;Name=Msa0034170-mRNA-1;_AED=0.27;_eAED=0.27;_QI=256|0.6|0.83|1|0.2|0.16|6|848|58
chr1_1 exon 62046789 62047367 62046789 ID=Msa0034170-mRNA-1:exon:19492;Parent=Msa0034170-mRNA-1
chr1_1 exon 62047469 62047797 62047469 ID=Msa0034170-mRNA-1:exon:19491;Parent=Msa0034170-mRNA-1
chr1_1 exon 62049154 62049214 62049154 ID=Msa0034170-mRNA-1:exon:19490;Parent=Msa0034170-mRNA-1
chr1_1 exon 62049459 62049484 62049459 ID=Msa0034170-mRNA-1:exon:19489;Parent=Msa0034170-mRNA-1
chr1_1 exon 62049690 62049876 62049690 ID=Msa0034170-mRNA-1:exon:19488;Parent=Msa0034170-mRNA-1
chr1_1 exon 62049992 62050090 62049992 ID=Msa0034170-mRNA-1:exon:19487;Parent=Msa0034170-mRNA-1
chr1_1 five_prime_UTR 62049992 62050090 62049992 ID=Msa0034170-mRNA-1:five_prime_utr;Parent=Msa0034170-mRNA-1
chr1_1 five_prime_UTR 62049720 62049876 62049720 ID=Msa0034170-mRNA-1:five_prime_utr;Parent=Msa0034170-mRNA-1
chr1_1 CDS 62049690 62049719 62049690 ID=Msa0034170-mRNA-1:cds;Parent=Msa0034170-mRNA-1
chr1_1 CDS 62049459 62049484 62049459 ID=Msa0034170-mRNA-1:cds;Parent=Msa0034170-mRNA-1
chr1_1 CDS 62049154 62049214 62049154 ID=Msa0034170-mRNA-1:cds;Parent=Msa0034170-mRNA-1
chr1_1 CDS 62047738 62047797 62047738 ID=Msa0034170-mRNA-1:cds;Parent=Msa0034170-mRNA-1
chr1_1 three_prime_UTR 62047469 62047737 62047469 ID=Msa0034170-mRNA-1:three_prime_utr;Parent=Msa0034170-mRNA-1
chr1_1 three_prime_UTR 62046789 62047367 62046789 ID=Msa0034170-mRNA-1:three_prime_utr;Parent=Msa0034170-mRNA-1
Gene Sequence

>Msa0034170

ATGGTCATTGAATTGAAACGAGGCGACGACAAGAAGGCTGCAATCTTGGGAAAAACGCACACAAAAGTGGCAGGGGATGATGACTCAAGACAATGTCTTTACTTTGATAAACACAAGTTGTCGCAAAGGGTGAAAGGAAACGACGAAGAGGAAAGGAAGATGGTTACATATCAGTAA
Protein sequence

>Msa0034170

MVIELKRGDDKKAAILGKTHTKVAGDDDSRQCLYFDKHKLSQRVKGNDEEERKMVTYQ*