AlfalfaGEDB | Msa0034450

M. sativa cultivar zhongmu-4 / Msa0034450

KEGG: -
GO: GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564
PFAM: PF19160.3

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0034450	sp\|P93050\|RKF3_ARATH	61.327	618	220	8	7	618	1	605	0.0	771

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0034450	Msa0102120	0.805140	1.541362e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0034450	MtrunA17_Chr1g0196391	97.393	652	16	1	1	652	1	651	0.0	1316
Msa0034450	MtrunA17_Chr7g0259211	65.528	615	197	7	28	640	21	622	0.0	811
Msa0034450	MtrunA17_Chr2g0277731	38.562	612	338	15	9	588	6	611	2.45e-122	377
Msa0034450	MtrunA17_Chr1g0159611	48.235	340	168	6	285	622	227	560	1.18e-106	334
Msa0034450	MtrunA17_Chr8g0380681	35.552	616	350	19	13	588	4	612	4.73e-99	330
Msa0034450	MtrunA17_Chr8g0380681	34.454	595	344	16	26	585	694	1277	1.31e-98	328
Msa0034450	MtrunA17_Chr2g0333181	42.029	345	188	5	285	623	28	366	2.12e-83	267
Msa0034450	MtrunA17_Chr2g0281971	42.812	313	167	5	267	577	23	325	1.41e-81	262
Msa0034450	MtrunA17_Chr5g0413511	45.151	299	155	2	283	577	45	338	8.13e-80	258
Msa0034450	MtrunA17_Chr4g0069251	42.857	315	171	3	285	596	167	475	1.08e-77	256

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0034450	AT2G48010.1	61.327	618	220	8	7	618	1	605	0.0	771
Msa0034450	AT1G11050.1	38.449	593	328	17	20	588	16	595	1.65e-118	367
Msa0034450	AT3G59110.1	43.686	293	158	2	285	575	178	465	6.32e-78	257
Msa0034450	AT3G24540.1	45.455	297	143	3	285	572	171	457	3.64e-77	254
Msa0034450	AT1G01540.2	38.218	348	203	4	237	577	89	431	1.07e-76	253
Msa0034450	AT3G24550.1	45.890	292	149	2	285	572	268	554	5.83e-76	256
Msa0034450	AT5G18500.2	44.027	293	157	2	285	575	154	441	6.34e-76	251
Msa0034450	AT5G18500.5	44.027	293	157	2	285	575	154	441	6.34e-76	251
Msa0034450	AT5G18500.4	44.027	293	157	2	285	575	154	441	6.34e-76	251
Msa0034450	AT5G18500.1	44.027	293	157	2	285	575	154	441	6.34e-76	251

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Find 185 sgRNAs with CRISPR-Local

Find 404 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTCCTCCACACCATGCT+GGG	0.513464	1_1:-62439801	Msa0034450:CDS
AAACTTTGCAAGTCCAAAAT+CGG	0.407026	1_1:+62438725	None:intergenic
AAAGCTGCAGCATAAATTGA+AGG	0.555519	1_1:+62439503	None:intergenic
AAATTTAATGATAAGGCAAT+TGG	0.332066	1_1:-62439311	Msa0034450:CDS
AACCTTCTGCTTTGACTGAT+TGG	0.277948	1_1:-62438527	Msa0034450:CDS
AAGGATCATGGTGCTAATAT+CGG	0.451882	1_1:-62439281	Msa0034450:CDS
AATAAACACTTAACAGTACC+AGG	0.591065	1_1:+62439455	None:intergenic
AATCACAAACAATAATCCTC+TGG	0.523694	1_1:+62438946	None:intergenic
AATGACTCATATGAGTACTA+GGG	0.620752	1_1:-62438691	Msa0034450:CDS
AATGATAAGGCAATTGGGGA+TGG	0.531225	1_1:-62439305	Msa0034450:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACAATAAGAACGAATGTTGA+AGG	+	chr1_1:62438429-62438448	None:intergenic	30.0%
TTCAACATTCGTTCTTATTG+TGG	-	chr1_1:62438428-62438447	Msa0034450:CDS	30.0%
GTGAGTTATCTTGAATTGAT+TGG	+	chr1_1:62438562-62438581	None:intergenic	30.0%
GGTAATTTATCTGATTGTAC+TGG	-	chr1_1:62438638-62438657	Msa0034450:CDS	30.0%
AATAAACACTTAACAGTACC+AGG	+	chr1_1:62438716-62438735	None:intergenic	30.0%
ATTGTGTTGTCGATAGTTAT+AGG	-	chr1_1:62438818-62438837	Msa0034450:CDS	30.0%
TTGTGTTGTCGATAGTTATA+GGG	-	chr1_1:62438819-62438838	Msa0034450:CDS	30.0%
AGGGATAATATAATTGGGAA+AGG	-	chr1_1:62439010-62439029	Msa0034450:CDS	30.0%
GGGATAATATAATTGGGAAA+GGG	-	chr1_1:62439011-62439030	Msa0034450:CDS	30.0%
GGATAATATAATTGGGAAAG+GGG	-	chr1_1:62439012-62439031	Msa0034450:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_1	gene	62438116	62440074	62438116	ID=Msa0034450;Name=Msa0034450
chr1_1	mRNA	62438116	62440074	62438116	ID=Msa0034450-mRNA-1;Parent=Msa0034450;Name=Msa0034450-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|652
chr1_1	exon	62438116	62440074	62438116	ID=Msa0034450-mRNA-1:exon:19733;Parent=Msa0034450-mRNA-1
chr1_1	CDS	62438116	62440074	62438116	ID=Msa0034450-mRNA-1:cds;Parent=Msa0034450-mRNA-1

Gene Sequence

>Msa0034450

ATGAACATGAACATGAACATGACAATGATTATGAACATCATCCTCGTCCTCATCTTCACCACCCTCTTAACAACTTCCCACTCCGCCACGTGTCCTCTCAACTTCACCCTCTTAACCACCCTCAGCGGCGGCAGAGCCACCTCAAAGCCACCATCTTCCTTCACCTCAACACGATGCCAGTTCGTCCGGCAAGCTCTCCGCCTTGTCCAATCCGACTACCTCCGCCGCACCGACTCTTTCATCCCTCCCTCAAACTCCTCCACACCATGCTGGGATTCTTTCCAATCCTACATCAACCAATTCGCACCAACCTTCAACATTCGTTCTTATTGTGGCTTCCAAACTGAATGGATCTCACAAGGTTGTGTCAACGTTACTACAAGACAACAATTTGAAGCACTTGTTCCTAAATCCACTATTCAAAATATGCAATCAAATTGTAACCAATCAATTCAAGATAACTCACCTTGTGCTCTTTGTACTACTAGTTTATCTGGTTTACCTTCTCTTGGTCAACCCATTGGTAATTTATCTGATTGTACTGGTTATCCTTCAATTTATGCTGCAGCTTTTGCCAATCAATTTGGACCGTCTGATCCTGGTACTGTTAAGTGTTTATTTCAACTTGATTTTACTTCAGGAGGTTCATCTGGTAGTAAGAAAAAGATTGTTGCTATTGTTGTTTCAATTGTTTCTGCTTTGATTGTGTTGTCGATAGTTATAGGGTTTTGGGCTTATAGGAAATTTAATGATAAGGCAATTGGGGATGGTAAGGATCATGGTGCTAATATCGGTGAGTCGGGTTCGGTTTCTGGGTTGGGTTCAATGGATAATAGTACTACTTTGATTAGGTTTTCAATTGATGATATTAAGAAGGCAACGAAGAATTTTCATAGGGATAATATAATTGGGAAAGGGGGTTATGGGAATGTGTATAAAGGGTTGTTGAATGATGGAAGTGAAGTTGCTTTTAAGAGGTTTAAGAATTGTTCTGCTGCTGGTGATACTAGTTTCACTCATGAGGTTGAGGTTATTGCTAGTGTTAGGCATGTGAATCTTGTTGCTTTGAGAGGTTATTGTAGTGCGACAACACGGTTAGAGGGTTACCAGAGGATTATTGTTTGTGATTTGATGAAAAATGGGAGTCTTCATGATCATTTGTTTGAATCTAATGGGGTGAAACTTAGTTGGCCGGCTCGTCAGAAGATTGCTCTTGGAACTGCTAGGGGATTATCTTATTTGCATTATGGAGCACAACCTGCCATCATTCATAGGGATATTAAAGCTAATAATATACTTTTGGATGATAAGTTTGAAGCTAAGGTTGCCGATTTTGGACTTGCAAAGTTTAATCCTGAGGGAATGACTCATATGAGTACTAGGGTTGCTGGAACTATGGGGTATGTTGCTCCTGAGTATGCTTTGTATGGACAATTAACAGAAAGGAGTGATGTGTTTAGTTTTGGTGTTGTGCTTCTTGAGTTATTGAGTGGAAGAAAGGCTCTTCAAACGAACGAAGATGGACAACCTTCTGCTTTGACTGATTGGGCTTGGTCATTGGTTAGAACGGGAAAGGCATTGGATGTTATTGAAGATGGGATGCCAGAACAAGGTTCAGATCAAGTTCTTGAGAAGTATGTGTTGATTGCTGTGCTTTGTTCTCATCCACAGTTATATGCTAGGCCAACAATGGATCAGGTTGTTAAAATGATGGAGACTGATGATGAATTAGTGCCCTCGATACCTGGACGGCCAATTCCTCTTGTTGCGGGGAGGCTTGATATTGAGAGATCTGTTAGCGGTTTTGGCTCCGGTCAGCTCTCTAGTCCTACAGGTTATCAATCGTTCACGTTAGAAATGGGAAGTGAACAAATGGAAAGTGAACGCCATTCTTCTAATCATTCTAGGGATGAAAGAAGCTCTGCCTCTAGGATTTTGAGTTCGGATTTGGATCAATGA

Protein sequence

>Msa0034450

MNMNMNMTMIMNIILVLIFTTLLTTSHSATCPLNFTLLTTLSGGRATSKPPSSFTSTRCQFVRQALRLVQSDYLRRTDSFIPPSNSSTPCWDSFQSYINQFAPTFNIRSYCGFQTEWISQGCVNVTTRQQFEALVPKSTIQNMQSNCNQSIQDNSPCALCTTSLSGLPSLGQPIGNLSDCTGYPSIYAAAFANQFGPSDPGTVKCLFQLDFTSGGSSGSKKKIVAIVVSIVSALIVLSIVIGFWAYRKFNDKAIGDGKDHGANIGESGSVSGLGSMDNSTTLIRFSIDDIKKATKNFHRDNIIGKGGYGNVYKGLLNDGSEVAFKRFKNCSAAGDTSFTHEVEVIASVRHVNLVALRGYCSATTRLEGYQRIIVCDLMKNGSLHDHLFESNGVKLSWPARQKIALGTARGLSYLHYGAQPAIIHRDIKANNILLDDKFEAKVADFGLAKFNPEGMTHMSTRVAGTMGYVAPEYALYGQLTERSDVFSFGVVLLELLSGRKALQTNEDGQPSALTDWAWSLVRTGKALDVIEDGMPEQGSDQVLEKYVLIAVLCSHPQLYARPTMDQVVKMMETDDELVPSIPGRPIPLVAGRLDIERSVSGFGSGQLSSPTGYQSFTLEMGSEQMESERHSSNHSRDERSSASRILSSDLDQ*