AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0036080

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0036080 MtrunA17_Chr1g0198171 96.107 488 11 4 1 485 1 483 0.0 967
Msa0036080 MtrunA17_Chr7g0265831 38.073 436 210 17 10 426 17 411 3.10e-76 247
Msa0036080 MtrunA17_Chr1g0179191 39.012 405 186 16 10 402 27 382 3.65e-74 243
Msa0036080 MtrunA17_Chr1g0201941 32.378 349 179 11 75 383 24 355 3.01e-42 159
Msa0036080 MtrunA17_Chr1g0201941 40.541 111 62 3 98 207 436 543 5.91e-19 90.1
Msa0036080 MtrunA17_Chr1g0201911 30.841 428 222 11 99 481 36 434 4.49e-35 138
Msa0036080 MtrunA17_Chr1g0201911 37.589 141 83 3 73 209 324 463 3.15e-19 90.9
Msa0036080 MtrunA17_Chr6g0463941 61.957 92 34 1 117 208 54 144 1.35e-31 128
Msa0036080 MtrunA17_Chr6g0463941 45.652 92 48 2 114 204 460 550 6.56e-19 90.1
Msa0036080 MtrunA17_Chr7g0273941 42.268 97 45 3 118 204 381 476 1.34e-13 72.8
Msa0036080 MtrunA17_Chr2g0290511 39.785 93 54 2 113 204 257 348 1.77e-13 72.0
Msa0036080 MtrunA17_Chr1g0190851 42.105 95 51 3 110 201 410 503 3.55e-12 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0036080 AT5G03680.1 36.571 525 259 16 5 491 9 497 5.23e-86 276
Msa0036080 AT3G10000.1 39.071 366 191 12 40 399 15 354 2.64e-65 219
Msa0036080 AT3G10000.2 37.158 366 188 13 40 399 15 344 6.18e-57 196
Msa0036080 AT1G76890.2 32.200 441 208 12 114 481 37 459 3.68e-49 178
Msa0036080 AT1G76890.2 40.000 160 78 8 88 233 360 515 1.22e-19 92.4
Msa0036080 AT1G76880.1 54.701 117 46 2 97 213 46 155 1.02e-30 126
Msa0036080 AT1G76880.1 45.361 97 51 2 114 209 404 499 6.55e-18 87.4
Msa0036080 AT1G33240.3 44.318 88 47 2 118 204 435 521 2.01e-15 79.3
Msa0036080 AT1G33240.1 44.318 88 47 2 118 204 435 521 2.06e-15 79.7
Msa0036080 AT1G33240.2 44.318 88 47 2 118 204 396 482 2.91e-15 79.0
Msa0036080 AT5G28300.1 39.216 102 50 4 118 208 459 559 3.55e-13 72.4

Find 115 sgRNAs with CRISPR-Local

Find 165 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTGGCTCTTACTCTTATT+TGG 0.155961 1_1:+64437445 None:intergenic
TTGTTGTATCATTTGCTTCT+TGG 0.177243 1_1:+64437428 None:intergenic
TTATTTATATGTTTGGATTA+AGG 0.199931 1_1:-64436766 None:intergenic
TGCATTTCTTTCCACTTCTT+TGG 0.203428 1_1:+64438136 None:intergenic
AGGATAATGGCTGAGGAATT+TGG 0.204963 1_1:-64438162 Msa0036080:CDS
CCTTGTGGAATGAAATTTCT+AGG 0.236619 1_1:-64439175 Msa0036080:intron
GAACATCAAGTAAGAGAAAA+AGG 0.239292 1_1:-64437316 Msa0036080:CDS
AGGTGAAAGGATGGTTTATA+TGG 0.259177 1_1:-64437263 Msa0036080:CDS
TTTGGTTCTGAGCATGAAAA+AGG 0.260495 1_1:-64437060 Msa0036080:three_prime_UTR
AAACACAAGGCCTTGGTGTT+TGG 0.270837 1_1:-64437154 Msa0036080:CDS
GAGCTGAGTGCAAGAAAATA+TGG 0.284773 1_1:-64437225 Msa0036080:CDS
AACACAAGGCCTTGGTGTTT+GGG 0.305639 1_1:-64437153 Msa0036080:CDS
TCTACTAGCTTTACCTTCCT+TGG 0.314459 1_1:+64438074 None:intergenic
GATCTTCTTGTTTAGGATAA+TGG 0.314988 1_1:-64438175 Msa0036080:intron
AAGTATATTATTTATATGTT+TGG 0.318515 1_1:-64436773 Msa0036080:three_prime_UTR
ATCATATTTATGAACTTTGT+AGG 0.329585 1_1:-64436851 Msa0036080:three_prime_UTR
TGGTTTATATGGGATTTAAT+AGG 0.331668 1_1:-64437252 Msa0036080:CDS
ATAGGGTCATAGGAATAATT+TGG 0.333734 1_1:+64439488 None:intergenic
ATCAAGAAATACATGAACTT+TGG 0.336932 1_1:-64437552 Msa0036080:CDS
GGATAATGGCTGAGGAATTT+GGG 0.344397 1_1:-64438161 Msa0036080:CDS
GGGTCATTGACTTGCAAGTT+TGG 0.355678 1_1:-64437039 Msa0036080:three_prime_UTR
ATGATATTAGTATGTCTCTT+AGG 0.361294 1_1:-64437201 Msa0036080:CDS
AAAACATCACCAACTATCAT+AGG 0.369956 1_1:+64439470 None:intergenic
GAGAAGATGAGTGACCTATA+TGG 0.374450 1_1:-64439617 Msa0036080:exon
TCAAGAAATACATGAACTTT+GGG 0.384682 1_1:-64437551 Msa0036080:CDS
GTAGCAACAAAGGAAGCATT+AGG 0.385971 1_1:+64437955 None:intergenic
GCAGCTATAGTGGTATTTGT+AGG 0.401800 1_1:+64439395 None:intergenic
GAAGTTGTAAGAAAACATAT+TGG 0.406391 1_1:-64437484 Msa0036080:CDS
GATGGAGGTTGGGATGAAAT+AGG 0.410977 1_1:-64437283 Msa0036080:CDS
GGTTTAGAAACTGCTGATAA+AGG 0.416775 1_1:-64439314 Msa0036080:CDS
TCCTATGACCCTATGATAGT+TGG 0.417353 1_1:-64439479 Msa0036080:CDS
AAGTTGTAAGAAAACATATT+GGG 0.418479 1_1:-64437483 Msa0036080:CDS
GATATTTGGATGATGGAGGT+TGG 0.425202 1_1:-64437294 Msa0036080:CDS
ATATTTGGATGATGGAGGTT+GGG 0.432092 1_1:-64437293 Msa0036080:CDS
TAGCAACAAAGGAAGCATTA+GGG 0.434533 1_1:+64437956 None:intergenic
GAGTGACCTATATGGGTTAC+CGG 0.435419 1_1:-64439609 Msa0036080:CDS
GAGGCATGAGAGTTTGTGTT+TGG 0.435521 1_1:+64437997 None:intergenic
GATCCTCCGGTAACCCATAT+AGG 0.435631 1_1:+64439603 None:intergenic
AGAAAGGGCAAAGTGGTCAT+AGG 0.438060 1_1:-64437750 Msa0036080:CDS
ATCAACCGCCGGAGATCCTC+CGG 0.444001 1_1:+64439590 None:intergenic
TATATGGGATTTAATAGGAA+TGG 0.452119 1_1:-64437247 Msa0036080:CDS
ACCAACTATCATAGGGTCAT+AGG 0.456713 1_1:+64439478 None:intergenic
TGCAGCAGCAGCAGCTATAG+TGG 0.457554 1_1:+64439385 None:intergenic
ATATTATCCACACTTTCAAA+AGG 0.458934 1_1:+64437394 None:intergenic
GCTTCTACTGCATTCTGTGT+AGG 0.459654 1_1:-64439335 Msa0036080:CDS
TATTCATAGATGGACAGAAA+TGG 0.466542 1_1:-64437374 Msa0036080:CDS
GAACCACCACCTTGTTTGTA+TGG 0.467332 1_1:-64439533 Msa0036080:CDS
AGAACAAGAGATGGCATCAA+AGG 0.471241 1_1:-64437611 Msa0036080:CDS
TTGATTCTTGTAACAACAGA+TGG 0.472038 1_1:-64439277 Msa0036080:CDS
TTTCAAACTTGATACAACTA+AGG 0.475487 1_1:-64437348 Msa0036080:CDS
AGAAGATGAGTGACCTATAT+GGG 0.480823 1_1:-64439616 Msa0036080:exon
ATTAACCAAGAAAATACTAA+TGG 0.483532 1_1:-64437913 Msa0036080:CDS
TTATTGAGACTCAAGATGCT+TGG 0.485535 1_1:-64437666 Msa0036080:CDS
ATGAAGTCGTCGAAGGAGAA+AGG 0.490837 1_1:-64437766 Msa0036080:CDS
GGTGAAAGGATGGTTTATAT+GGG 0.497182 1_1:-64437262 Msa0036080:CDS
AAACAAGGTGGTGGTTCAGC+TGG 0.498191 1_1:+64439539 None:intergenic
GTGTGGATAATATTCATAGA+TGG 0.500320 1_1:-64437384 Msa0036080:CDS
CTTGTTTAGGATAATGGCTG+AGG 0.502416 1_1:-64438169 Msa0036080:intron
ATAGGAGGGCAAGGAAAAGT+TGG 0.504848 1_1:-64437732 Msa0036080:CDS
TATTATCCACACTTTCAAAA+GGG 0.515340 1_1:+64437395 None:intergenic
ACTGCTGATAAAGGATGGCT+TGG 0.518070 1_1:-64439305 Msa0036080:CDS
ATGGGTTACCGGAGGATCTC+CGG 0.518191 1_1:-64439598 Msa0036080:CDS
CAGCTATAGTGGTATTTGTA+GGG 0.519351 1_1:+64439396 None:intergenic
TGAGACTCAAGATGCTTGGA+TGG 0.522893 1_1:-64437662 Msa0036080:CDS
GGATGAAATAGGTGAAAGGA+TGG 0.528733 1_1:-64437272 Msa0036080:CDS
AGACAGCTTGAAGCAATATG+TGG 0.533898 1_1:-64438024 Msa0036080:CDS
TGAAGTCGTCGAAGGAGAAA+GGG 0.536001 1_1:-64437765 Msa0036080:CDS
TGGCATCAAAGGAAGAAGAA+AGG 0.537348 1_1:-64437600 Msa0036080:CDS
TTAGACTTTGTGAATACTTG+TGG 0.538073 1_1:+64437866 None:intergenic
GGAAGAGAAAAGAGTCAATG+AGG 0.550829 1_1:-64437579 Msa0036080:CDS
AGAGAAAGAGTCTCTTGTCT+AGG 0.553492 1_1:+64439254 None:intergenic
TAGAAACTGCTGATAAAGGA+TGG 0.556731 1_1:-64439310 Msa0036080:CDS
AAACATCACCAACTATCATA+GGG 0.561437 1_1:+64439471 None:intergenic
TTGAGTTTGAGTAGCAACAA+AGG 0.562015 1_1:+64437945 None:intergenic
AAGGGCAAAGTGGTCATAGG+AGG 0.562963 1_1:-64437747 Msa0036080:CDS
GGTATTTGTAGGGTTAACAG+TGG 0.564956 1_1:+64439406 None:intergenic
GGTAAAGCTAGTAGACAAGA+TGG 0.568681 1_1:-64438066 Msa0036080:CDS
AAAGGATATTTGGATGATGG+AGG 0.568757 1_1:-64437298 Msa0036080:CDS
CCTAGAAATTTCATTCCACA+AGG 0.573860 1_1:+64439175 None:intergenic
TCATAGGAATAATTTGGTGG+AGG 0.577820 1_1:+64439494 None:intergenic
GAATTTGGGTACCAAAGAAG+TGG 0.582190 1_1:-64438147 Msa0036080:CDS
CAGTGGAGGAATAGTCATAG+TGG 0.586600 1_1:+64439423 None:intergenic
TTGATGATGTTATCAACCGC+CGG 0.588660 1_1:+64439579 None:intergenic
AGGGCAAGGAAAAGTTGGAG+AGG 0.591096 1_1:-64437727 Msa0036080:CDS
TATTAGTATGTCTCTTAGGA+AGG 0.591430 1_1:-64437197 Msa0036080:CDS
ATTTGTAGGGTTAACAGTGG+AGG 0.594970 1_1:+64439409 None:intergenic
AGCTGAGTGCAAGAAAATAT+GGG 0.595363 1_1:-64437224 Msa0036080:CDS
TATTACAAGAAGACCAAGGA+AGG 0.601931 1_1:-64438087 Msa0036080:CDS
GGGTCATAGGAATAATTTGG+TGG 0.603660 1_1:+64439491 None:intergenic
AAGTTGGAGAGGTAAAGTTG+AGG 0.607935 1_1:-64437716 Msa0036080:CDS
ATAATCAGAAAGCACCCTTG+TGG 0.619380 1_1:-64439190 Msa0036080:CDS
AGGTGTGTAAGACTCTAGCT+AGG 0.621926 1_1:-64437000 Msa0036080:three_prime_UTR
GTTGGGATGAAATAGGTGAA+AGG 0.623102 1_1:-64437276 Msa0036080:CDS
AAAGTGGTCATAGGAGGGCA+AGG 0.623960 1_1:-64437741 Msa0036080:CDS
GGTGAGTGAAGCTACTACGA+GGG 0.624158 1_1:+64439444 None:intergenic
TGAACAAAGAGAACAAGAGA+TGG 0.625612 1_1:-64437620 Msa0036080:CDS
AGCAACAAAGGAAGCATTAG+GGG 0.629023 1_1:+64437957 None:intergenic
AAGAAGTGGAAAGAAATGCA+AGG 0.641850 1_1:-64438133 Msa0036080:CDS
AGGGCAAAGTGGTCATAGGA+GGG 0.643035 1_1:-64437746 Msa0036080:CDS
TTGGTTCATTGAAAATCACA+AGG 0.643246 1_1:-64437020 Msa0036080:three_prime_UTR
TTAGGAAGGACAGTGGACTG+TGG 0.656609 1_1:-64437183 Msa0036080:CDS
ACACACTATGAAGTCGTCGA+AGG 0.660577 1_1:-64437773 Msa0036080:CDS
GCACCATACAAACAAGGTGG+TGG 0.666201 1_1:+64439530 None:intergenic
CTCATCTGAAAACAACGATG+AGG 0.668787 1_1:-64437812 Msa0036080:CDS
AGCTTAAGTCCCAAACACCA+AGG 0.671681 1_1:+64437144 None:intergenic
AACTTGATACAACTAAGGAG+TGG 0.671997 1_1:-64437343 Msa0036080:CDS
TCGAAGGAGAAAGGGCAAAG+TGG 0.682624 1_1:-64437757 Msa0036080:CDS
ATGTCTCTTAGGAAGGACAG+TGG 0.682744 1_1:-64437190 Msa0036080:CDS
GCAGCAGCACCATACAAACA+AGG 0.705312 1_1:+64439524 None:intergenic
CAAGTATTACAAGAAGACCA+AGG 0.709647 1_1:-64438091 Msa0036080:CDS
CTAGAAATTTCATTCCACAA+GGG 0.711484 1_1:+64439176 None:intergenic
TGACCTATATGGGTTACCGG+AGG 0.716927 1_1:-64439606 Msa0036080:CDS
TGGTGAGTGAAGCTACTACG+AGG 0.746302 1_1:+64439443 None:intergenic
GGTTACCGGAGGATCTCCGG+CGG 0.758338 1_1:-64439595 Msa0036080:CDS
GCAGCACCATACAAACAAGG+TGG 0.760932 1_1:+64439527 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATTTCACTTTTTTTTT+TGG - chr1_1:64437316-64437335 Msa0036080:CDS 10.0%
!! TTAAATTTAAATTGTAGTTA+CGG - chr1_1:64437953-64437972 Msa0036080:CDS 10.0%
!! AAGTATATTATTTATATGTT+TGG - chr1_1:64439655-64439674 Msa0036080:five_prime_UTR 10.0%
!! ATCATAATAAAAAAAACACA+TGG + chr1_1:64437429-64437448 None:intergenic 15.0%
!! TTTAAACTTAAAAAAACACA+TGG + chr1_1:64439454-64439473 None:intergenic 15.0%
!!! GTGTTTTTTTAAGTTTAAAT+AGG - chr1_1:64439455-64439474 Msa0036080:CDS 15.0%
!! TTAGTGTCTTAATTAATTAC+TGG - chr1_1:64437282-64437301 Msa0036080:CDS 20.0%
!! GACAAAAAAGAAAATTATCA+TGG + chr1_1:64437381-64437400 None:intergenic 20.0%
!!! TTTTGTTTGAAAATATCATG+TGG - chr1_1:64437788-64437807 Msa0036080:CDS 20.0%
!! ATTAACCAAGAAAATACTAA+TGG - chr1_1:64438515-64438534 Msa0036080:intron 20.0%
!! AAGTTGTAAGAAAACATATT+GGG - chr1_1:64438945-64438964 Msa0036080:intron 20.0%
!!! TTTTACATTAATTGTGTCAA+AGG + chr1_1:64439325-64439344 None:intergenic 20.0%
!! ATCATATTTATGAACTTTGT+AGG - chr1_1:64439577-64439596 Msa0036080:CDS 20.0%
! CAATGAATGATTTAGTATCA+TGG + chr1_1:64437523-64437542 None:intergenic 25.0%
! AGTATATACAAAACAAAGAC+AGG + chr1_1:64437579-64437598 None:intergenic 25.0%
!! ATTTTCTTGGTTAATTGTGA+AGG + chr1_1:64438510-64438529 None:intergenic 25.0%
! ATCAAGAAATACATGAACTT+TGG - chr1_1:64438876-64438895 Msa0036080:intron 25.0%
! TCAAGAAATACATGAACTTT+GGG - chr1_1:64438877-64438896 Msa0036080:intron 25.0%
! GAAGTTGTAAGAAAACATAT+TGG - chr1_1:64438944-64438963 Msa0036080:intron 25.0%
! TATTATCCACACTTTCAAAA+GGG + chr1_1:64439036-64439055 None:intergenic 25.0%
! ATATTATCCACACTTTCAAA+AGG + chr1_1:64439037-64439056 None:intergenic 25.0%
! TTTCAAACTTGATACAACTA+AGG - chr1_1:64439080-64439099 Msa0036080:intron 25.0%
! AGTAAGAGAAAAAGGATATT+TGG - chr1_1:64439120-64439139 Msa0036080:intron 25.0%
! TGGTTTATATGGGATTTAAT+AGG - chr1_1:64439176-64439195 Msa0036080:CDS 25.0%
! TATATGGGATTTAATAGGAA+TGG - chr1_1:64439181-64439200 Msa0036080:CDS 25.0%
! ATGATATTAGTATGTCTCTT+AGG - chr1_1:64439227-64439246 Msa0036080:CDS 25.0%
ATAGGGTCATAGGAATAATT+TGG + chr1_1:64436943-64436962 None:intergenic 30.0%
AAACATCACCAACTATCATA+GGG + chr1_1:64436960-64436979 None:intergenic 30.0%
AAAACATCACCAACTATCAT+AGG + chr1_1:64436961-64436980 None:intergenic 30.0%
TTGATTCTTGTAACAACAGA+TGG - chr1_1:64437151-64437170 Msa0036080:CDS 30.0%
CTAGAAATTTCATTCCACAA+GGG + chr1_1:64437255-64437274 None:intergenic 30.0%
!!! CTGTCATTTTCAATCACAAA+AGG + chr1_1:64437350-64437369 None:intergenic 30.0%
! CTTTTGTGATTGAAAATGAC+AGG - chr1_1:64437348-64437367 Msa0036080:CDS 30.0%
GATACTAAATCATTCATTGC+AGG - chr1_1:64437524-64437543 Msa0036080:CDS 30.0%
! TGTTAGTGTTTTGTACTGAA+AGG + chr1_1:64437730-64437749 None:intergenic 30.0%
AAATCAAGTGATACTGTGAT+AGG + chr1_1:64437764-64437783 None:intergenic 30.0%
TTGAAAATATCATGTGGTCT+TGG - chr1_1:64437794-64437813 Msa0036080:CDS 30.0%
TGATTGTAATTGAAACCTTG+GGG + chr1_1:64437845-64437864 None:intergenic 30.0%
GTGATTGTAATTGAAACCTT+GGG + chr1_1:64437846-64437865 None:intergenic 30.0%
TGTGATTGTAATTGAAACCT+TGG + chr1_1:64437847-64437866 None:intergenic 30.0%
! GATCTTCTTGTTTAGGATAA+TGG - chr1_1:64438253-64438272 Msa0036080:intron 30.0%
!! GGTAATTTTGTCTAAAGTTG+AGG + chr1_1:64438453-64438472 None:intergenic 30.0%
! CAACACCATTAGTATTTTCT+TGG + chr1_1:64438523-64438542 None:intergenic 30.0%
TTAGACTTTGTGAATACTTG+TGG + chr1_1:64438565-64438584 None:intergenic 30.0%
TTGTTGTATCATTTGCTTCT+TGG + chr1_1:64439003-64439022 None:intergenic 30.0%
GTGTGGATAATATTCATAGA+TGG - chr1_1:64439044-64439063 Msa0036080:intron 30.0%
TATTCATAGATGGACAGAAA+TGG - chr1_1:64439054-64439073 Msa0036080:intron 30.0%
GAACATCAAGTAAGAGAAAA+AGG - chr1_1:64439112-64439131 Msa0036080:intron 30.0%
GAAAAAGGATATTTGGATGA+TGG - chr1_1:64439127-64439146 Msa0036080:intron 30.0%
TATTAGTATGTCTCTTAGGA+AGG - chr1_1:64439231-64439250 Msa0036080:CDS 30.0%
TTGGTTCATTGAAAATCACA+AGG - chr1_1:64439408-64439427 Msa0036080:CDS 30.0%
!! AATAGGTTTTATGTGTACGT+AGG - chr1_1:64439472-64439491 Msa0036080:CDS 30.0%
AGAAGATGAGTGACCTATAT+GGG - chr1_1:64436812-64436831 Msa0036080:three_prime_UTR 35.0%
! TCATAGGAATAATTTGGTGG+AGG + chr1_1:64436937-64436956 None:intergenic 35.0%
CAGCTATAGTGGTATTTGTA+GGG + chr1_1:64437035-64437054 None:intergenic 35.0%
GGTTTAGAAACTGCTGATAA+AGG - chr1_1:64437114-64437133 Msa0036080:three_prime_UTR 35.0%
TAGAAACTGCTGATAAAGGA+TGG - chr1_1:64437118-64437137 Msa0036080:three_prime_UTR 35.0%
CCTAGAAATTTCATTCCACA+AGG + chr1_1:64437256-64437275 None:intergenic 35.0%
CCTTGTGGAATGAAATTTCT+AGG - chr1_1:64437253-64437272 Msa0036080:CDS 35.0%
TTCATTGCAGGTGCAAATTA+AGG - chr1_1:64437536-64437555 Msa0036080:CDS 35.0%
! TAGTACAACTTTTTACCCCA+AGG - chr1_1:64437827-64437846 Msa0036080:CDS 35.0%
TATCCCACAAATCTTTGCAT+TGG - chr1_1:64437891-64437910 Msa0036080:CDS 35.0%
GCACAAAGCTGTAATAAATG+TGG - chr1_1:64438062-64438081 Msa0036080:CDS 35.0%
! GCTTTGTGATCTTCTTGTTT+AGG - chr1_1:64438246-64438265 Msa0036080:intron 35.0%
TGCATTTCTTTCCACTTCTT+TGG + chr1_1:64438295-64438314 None:intergenic 35.0%
AAGAAGTGGAAAGAAATGCA+AGG - chr1_1:64438295-64438314 Msa0036080:intron 35.0%
CAAGTATTACAAGAAGACCA+AGG - chr1_1:64438337-64438356 Msa0036080:intron 35.0%
TATTACAAGAAGACCAAGGA+AGG - chr1_1:64438341-64438360 Msa0036080:intron 35.0%
!! TAGCAACAAAGGAAGCATTA+GGG + chr1_1:64438475-64438494 None:intergenic 35.0%
TTGAGTTTGAGTAGCAACAA+AGG + chr1_1:64438486-64438505 None:intergenic 35.0%
TTATTGAGACTCAAGATGCT+TGG - chr1_1:64438762-64438781 Msa0036080:intron 35.0%
TGAACAAAGAGAACAAGAGA+TGG - chr1_1:64438808-64438827 Msa0036080:intron 35.0%
TTTGGGCTAAAGAAAAAGCA+TGG - chr1_1:64438894-64438913 Msa0036080:intron 35.0%
! TTGGGCTAAAGAAAAAGCAT+GGG - chr1_1:64438895-64438914 Msa0036080:intron 35.0%
! TCTTGGCTCTTACTCTTATT+TGG + chr1_1:64438986-64439005 None:intergenic 35.0%
!! TGAATACCCTTTTGAAAGTG+TGG - chr1_1:64439027-64439046 Msa0036080:intron 35.0%
AACTTGATACAACTAAGGAG+TGG - chr1_1:64439085-64439104 Msa0036080:intron 35.0%
! AAAGGATATTTGGATGATGG+AGG - chr1_1:64439130-64439149 Msa0036080:intron 35.0%
!! ATATTTGGATGATGGAGGTT+GGG - chr1_1:64439135-64439154 Msa0036080:intron 35.0%
AGGTGAAAGGATGGTTTATA+TGG - chr1_1:64439165-64439184 Msa0036080:intron 35.0%
GGTGAAAGGATGGTTTATAT+GGG - chr1_1:64439166-64439185 Msa0036080:intron 35.0%
AGCTGAGTGCAAGAAAATAT+GGG - chr1_1:64439204-64439223 Msa0036080:CDS 35.0%
!!! GCACATGAATCAGAGTTTTT+TGG - chr1_1:64439350-64439369 Msa0036080:CDS 35.0%
!! TTTGGTTCTGAGCATGAAAA+AGG - chr1_1:64439368-64439387 Msa0036080:CDS 35.0%
!! TTGGTTCTGAGCATGAAAAA+GGG - chr1_1:64439369-64439388 Msa0036080:CDS 35.0%
GAGAAGATGAGTGACCTATA+TGG - chr1_1:64436811-64436830 Msa0036080:three_prime_UTR 40.0%
!! TTGATGATGTTATCAACCGC+CGG + chr1_1:64436852-64436871 None:intergenic 40.0%
GGGTCATAGGAATAATTTGG+TGG + chr1_1:64436940-64436959 None:intergenic 40.0%
TCCTATGACCCTATGATAGT+TGG - chr1_1:64436949-64436968 Msa0036080:three_prime_UTR 40.0%
ACCAACTATCATAGGGTCAT+AGG + chr1_1:64436953-64436972 None:intergenic 40.0%
ATTTGTAGGGTTAACAGTGG+AGG + chr1_1:64437022-64437041 None:intergenic 40.0%
GGTATTTGTAGGGTTAACAG+TGG + chr1_1:64437025-64437044 None:intergenic 40.0%
GCAGCTATAGTGGTATTTGT+AGG + chr1_1:64437036-64437055 None:intergenic 40.0%
AGAGAAAGAGTCTCTTGTCT+AGG + chr1_1:64437177-64437196 None:intergenic 40.0%
!! ATAATCAGAAAGCACCCTTG+TGG - chr1_1:64437238-64437257 Msa0036080:CDS 40.0%
GTGACTATACTTACTTGCAC+TGG - chr1_1:64437490-64437509 Msa0036080:CDS 40.0%
GCTGAGCGAATTAGGTTAAT+AGG + chr1_1:64437607-64437626 None:intergenic 40.0%
CCTCCAATGCAAAGATTTGT+GGG + chr1_1:64437897-64437916 None:intergenic 40.0%
TCCTCCAATGCAAAGATTTG+TGG + chr1_1:64437898-64437917 None:intergenic 40.0%
TTTGCATTGGAGGAGATCAA+AGG - chr1_1:64437904-64437923 Msa0036080:CDS 40.0%
TTGCATTGGAGGAGATCAAA+GGG - chr1_1:64437905-64437924 Msa0036080:CDS 40.0%
! CTTGTTTAGGATAATGGCTG+AGG - chr1_1:64438259-64438278 Msa0036080:intron 40.0%
! AGGATAATGGCTGAGGAATT+TGG - chr1_1:64438266-64438285 Msa0036080:intron 40.0%
! GGATAATGGCTGAGGAATTT+GGG - chr1_1:64438267-64438286 Msa0036080:intron 40.0%
GAATTTGGGTACCAAAGAAG+TGG - chr1_1:64438281-64438300 Msa0036080:intron 40.0%
!! TCTACTAGCTTTACCTTCCT+TGG + chr1_1:64438357-64438376 None:intergenic 40.0%
GGTAAAGCTAGTAGACAAGA+TGG - chr1_1:64438362-64438381 Msa0036080:intron 40.0%
AGACAGCTTGAAGCAATATG+TGG - chr1_1:64438404-64438423 Msa0036080:intron 40.0%
!! AGCAACAAAGGAAGCATTAG+GGG + chr1_1:64438474-64438493 None:intergenic 40.0%
!! GTAGCAACAAAGGAAGCATT+AGG + chr1_1:64438476-64438495 None:intergenic 40.0%
!! CTCATCGTTGTTTTCAGATG+AGG + chr1_1:64438618-64438637 None:intergenic 40.0%
CTCATCTGAAAACAACGATG+AGG - chr1_1:64438616-64438635 Msa0036080:intron 40.0%
AAGTTGGAGAGGTAAAGTTG+AGG - chr1_1:64438712-64438731 Msa0036080:intron 40.0%
AGAACAAGAGATGGCATCAA+AGG - chr1_1:64438817-64438836 Msa0036080:intron 40.0%
TGGCATCAAAGGAAGAAGAA+AGG - chr1_1:64438828-64438847 Msa0036080:intron 40.0%
GGAAGAGAAAAGAGTCAATG+AGG - chr1_1:64438849-64438868 Msa0036080:intron 40.0%
!! GATATTTGGATGATGGAGGT+TGG - chr1_1:64439134-64439153 Msa0036080:intron 40.0%
GTTGGGATGAAATAGGTGAA+AGG - chr1_1:64439152-64439171 Msa0036080:intron 40.0%
GGATGAAATAGGTGAAAGGA+TGG - chr1_1:64439156-64439175 Msa0036080:intron 40.0%
GAGCTGAGTGCAAGAAAATA+TGG - chr1_1:64439203-64439222 Msa0036080:CDS 40.0%
ACTGTGGAGTCAAAAACACA+AGG - chr1_1:64439261-64439280 Msa0036080:CDS 40.0%
! GAGTGACCTATATGGGTTAC+CGG - chr1_1:64436819-64436838 Msa0036080:three_prime_UTR 45.0%
! GAACCACCACCTTGTTTGTA+TGG - chr1_1:64436895-64436914 Msa0036080:three_prime_UTR 45.0%
CAGTGGAGGAATAGTCATAG+TGG + chr1_1:64437008-64437027 None:intergenic 45.0%
GCTTCTACTGCATTCTGTGT+AGG - chr1_1:64437093-64437112 Msa0036080:three_prime_UTR 45.0%
ACTGCTGATAAAGGATGGCT+TGG - chr1_1:64437123-64437142 Msa0036080:three_prime_UTR 45.0%
AAACTCCAATAGCCCTAGCT+AGG + chr1_1:64437632-64437651 None:intergenic 45.0%
CCCACAAATCTTTGCATTGG+AGG - chr1_1:64437894-64437913 Msa0036080:CDS 45.0%
GAGGCATGAGAGTTTGTGTT+TGG + chr1_1:64438434-64438453 None:intergenic 45.0%
ACACACTATGAAGTCGTCGA+AGG - chr1_1:64438655-64438674 Msa0036080:intron 45.0%
ATGAAGTCGTCGAAGGAGAA+AGG - chr1_1:64438662-64438681 Msa0036080:intron 45.0%
TGAAGTCGTCGAAGGAGAAA+GGG - chr1_1:64438663-64438682 Msa0036080:intron 45.0%
AGAAAGGGCAAAGTGGTCAT+AGG - chr1_1:64438678-64438697 Msa0036080:intron 45.0%
ATAGGAGGGCAAGGAAAAGT+TGG - chr1_1:64438696-64438715 Msa0036080:intron 45.0%
TGAGACTCAAGATGCTTGGA+TGG - chr1_1:64438766-64438785 Msa0036080:intron 45.0%
GATGGAGGTTGGGATGAAAT+AGG - chr1_1:64439145-64439164 Msa0036080:intron 45.0%
ATGTCTCTTAGGAAGGACAG+TGG - chr1_1:64439238-64439257 Msa0036080:CDS 45.0%
GAGTCAAAAACACAAGGCCT+TGG - chr1_1:64439267-64439286 Msa0036080:CDS 45.0%
!! AAACACAAGGCCTTGGTGTT+TGG - chr1_1:64439274-64439293 Msa0036080:CDS 45.0%
!! AACACAAGGCCTTGGTGTTT+GGG - chr1_1:64439275-64439294 Msa0036080:CDS 45.0%
AGCTTAAGTCCCAAACACCA+AGG + chr1_1:64439287-64439306 None:intergenic 45.0%
!! GGGTCATTGACTTGCAAGTT+TGG - chr1_1:64439389-64439408 Msa0036080:CDS 45.0%
AGGTGTGTAAGACTCTAGCT+AGG - chr1_1:64439428-64439447 Msa0036080:CDS 45.0%
! TGACCTATATGGGTTACCGG+AGG - chr1_1:64436822-64436841 Msa0036080:three_prime_UTR 50.0%
GATCCTCCGGTAACCCATAT+AGG + chr1_1:64436828-64436847 None:intergenic 50.0%
! AAACAAGGTGGTGGTTCAGC+TGG + chr1_1:64436892-64436911 None:intergenic 50.0%
GCACCATACAAACAAGGTGG+TGG + chr1_1:64436901-64436920 None:intergenic 50.0%
GCAGCACCATACAAACAAGG+TGG + chr1_1:64436904-64436923 None:intergenic 50.0%
GCAGCAGCACCATACAAACA+AGG + chr1_1:64436907-64436926 None:intergenic 50.0%
GGTGAGTGAAGCTACTACGA+GGG + chr1_1:64436987-64437006 None:intergenic 50.0%
TGGTGAGTGAAGCTACTACG+AGG + chr1_1:64436988-64437007 None:intergenic 50.0%
GCTAGGTAGCTGAGCGAATT+AGG + chr1_1:64437615-64437634 None:intergenic 50.0%
ATTCGCTCAGCTACCTAGCT+AGG - chr1_1:64437616-64437635 Msa0036080:CDS 50.0%
TTCGCTCAGCTACCTAGCTA+GGG - chr1_1:64437617-64437636 Msa0036080:CDS 50.0%
AGCTACCTAGCTAGGGCTAT+TGG - chr1_1:64437624-64437643 Msa0036080:CDS 50.0%
TCGAAGGAGAAAGGGCAAAG+TGG - chr1_1:64438671-64438690 Msa0036080:intron 50.0%
AAGGGCAAAGTGGTCATAGG+AGG - chr1_1:64438681-64438700 Msa0036080:intron 50.0%
AGGGCAAAGTGGTCATAGGA+GGG - chr1_1:64438682-64438701 Msa0036080:intron 50.0%
AAAGTGGTCATAGGAGGGCA+AGG - chr1_1:64438687-64438706 Msa0036080:intron 50.0%
AGGGCAAGGAAAAGTTGGAG+AGG - chr1_1:64438701-64438720 Msa0036080:intron 50.0%
!! TTAGGAAGGACAGTGGACTG+TGG - chr1_1:64439245-64439264 Msa0036080:CDS 50.0%
! ATGGGTTACCGGAGGATCTC+CGG - chr1_1:64436830-64436849 Msa0036080:three_prime_UTR 55.0%
TGCAGCAGCAGCAGCTATAG+TGG + chr1_1:64437046-64437065 None:intergenic 55.0%
ATCAACCGCCGGAGATCCTC+CGG + chr1_1:64436841-64436860 None:intergenic 60.0%
GGTTACCGGAGGATCTCCGG+CGG - chr1_1:64436833-64436852 Msa0036080:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 64436768 64439682 64436768 ID=Msa0036080;Name=Msa0036080
chr1_1 mRNA 64436768 64439682 64436768 ID=Msa0036080-mRNA-1;Parent=Msa0036080;Name=Msa0036080-mRNA-1;_AED=0.00;_eAED=0.00;_QI=49|1|1|1|1|1|2|379|497
chr1_1 exon 64436768 64438182 64436768 ID=Msa0036080-mRNA-1:exon:20648;Parent=Msa0036080-mRNA-1
chr1_1 exon 64439176 64439682 64439176 ID=Msa0036080-mRNA-1:exon:20647;Parent=Msa0036080-mRNA-1
chr1_1 five_prime_UTR 64439634 64439682 64439634 ID=Msa0036080-mRNA-1:five_prime_utr;Parent=Msa0036080-mRNA-1
chr1_1 CDS 64439176 64439633 64439176 ID=Msa0036080-mRNA-1:cds;Parent=Msa0036080-mRNA-1
chr1_1 CDS 64437147 64438182 64437147 ID=Msa0036080-mRNA-1:cds;Parent=Msa0036080-mRNA-1
chr1_1 three_prime_UTR 64436768 64437146 64436768 ID=Msa0036080-mRNA-1:three_prime_utr;Parent=Msa0036080-mRNA-1
Gene Sequence

>Msa0036080

ATGAGTGACCTATATGGGTTACCGGAGGATCTCCGGCGGTTGATAACATCATCAAGAAACACTACACATCTACCAGCTGAACCACCACCTTGTTTGTATGGTGCTGCTGCTACTTTTCCTCCACCAAATTATTCCTATGACCCTATGATAGTTGGTGATGTTTTCTACCCTCGTAGTAGCTTCACTCACCACTATGACTATTCCTCCACTGTTAACCCTACAAATACCACTATAGCTGCTGCTGCTGCAGCAGCTTCTAATTCAGATCATGCTACTGCTTCTACTGCATTCTGTGTAGGTTTAGAAACTGCTGATAAAGGATGGCTTGGTAACTTTGATTCTTGTAACAACAGATGGCCTAGACAAGAGACTCTTTCTCTTCTTGAAATCAGATCTCGTCTTGATTCTAAGTTCAGAGAGAATAATCAGAAAGCACCCTTGTGGAATGAAATTTCTAGGATAATGGCTGAGGAATTTGGGTACCAAAGAAGTGGAAAGAAATGCAAGGAAAAGTTTGAGAATTTGTACAAGTATTACAAGAAGACCAAGGAAGGTAAAGCTAGTAGACAAGATGGTAAGCACTATAGATTTTTCAGACAGCTTGAAGCAATATGTGGAGAACCAAACACAAACTCTCATGCCTCAACTTTAGACAAAATTACCCCTAATGCTTCCTTTGTTGCTACTCAAACTCAAACCTTCACAATTAACCAAGAAAATACTAATGGTGTTGATCACAATTTGATCAATAACCACAAGTATTCACAAAGTCTAAGTTTCTCGAACTCGTCTGAATTTGAGACATCCTCATCTGAAAACAACGATGAGGATCTCTCAACTATTGCACACACTATGAAGTCGTCGAAGGAGAAAGGGCAAAGTGGTCATAGGAGGGCAAGGAAAAGTTGGAGAGGTAAAGTTGAGGAGATTGTAGATTCTCACATGAAAAATATTATTGAGACTCAAGATGCTTGGATGGAGAGAATGTTGAGTGTTGTTGAACAAAGAGAACAAGAGATGGCATCAAAGGAAGAAGAAAGGAAGAGAAAAGAGTCAATGAGGTTTGATCAAGAAATACATGAACTTTGGGCTAAAGAAAAAGCATGGGTTGAAGCAAGAGATTCTGCATTGTTAGAAGTTGTAAGAAAACATATTGGGATACAACTTGAAGCACCAAATAAGAGTAAGAGCCAAGAAGCAAATGATACAACAAATTATGAATACCCTTTTGAAAGTGTGGATAATATTCATAGATGGACAGAAATGGAAATTTCAAACTTGATACAACTAAGGAGTGGTTTTGAACATCAAGTAAGAGAAAAAGGATATTTGGATGATGGAGGTTGGGATGAAATAGGTGAAAGGATGGTTTATATGGGATTTAATAGGAATGGAGCTGAGTGCAAGAAAATATGGGATGATATTAGTATGTCTCTTAGGAAGGACAGTGGACTGTGGAGTCAAAAACACAAGGCCTTGGTGTTTGGGACTTAA
Protein sequence

>Msa0036080

MSDLYGLPEDLRRLITSSRNTTHLPAEPPPCLYGAAATFPPPNYSYDPMIVGDVFYPRSSFTHHYDYSSTVNPTNTTIAAAAAAASNSDHATASTAFCVGLETADKGWLGNFDSCNNRWPRQETLSLLEIRSRLDSKFRENNQKAPLWNEISRIMAEEFGYQRSGKKCKEKFENLYKYYKKTKEGKASRQDGKHYRFFRQLEAICGEPNTNSHASTLDKITPNASFVATQTQTFTINQENTNGVDHNLINNHKYSQSLSFSNSSEFETSSSENNDEDLSTIAHTMKSSKEKGQSGHRRARKSWRGKVEEIVDSHMKNIIETQDAWMERMLSVVEQREQEMASKEEERKRKESMRFDQEIHELWAKEKAWVEARDSALLEVVRKHIGIQLEAPNKSKSQEANDTTNYEYPFESVDNIHRWTEMEISNLIQLRSGFEHQVREKGYLDDGGWDEIGERMVYMGFNRNGAECKKIWDDISMSLRKDSGLWSQKHKALVFGT*