AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0130160

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0130160 MtrunA17_Chr1g0198171 97.431 506 7 3 1 505 1 501 0.0 1026
Msa0130160 MtrunA17_Chr7g0265831 41.071 392 174 17 10 386 17 366 4.02e-75 244
Msa0130160 MtrunA17_Chr1g0179191 39.012 405 186 16 10 402 27 382 3.48e-74 243
Msa0130160 MtrunA17_Chr1g0201941 34.853 307 153 10 117 383 56 355 5.62e-42 159
Msa0130160 MtrunA17_Chr1g0201941 40.541 111 62 3 98 207 436 543 3.82e-19 90.9
Msa0130160 MtrunA17_Chr1g0201911 30.969 423 218 11 99 476 36 429 1.01e-35 140
Msa0130160 MtrunA17_Chr1g0201911 47.525 101 51 2 110 209 364 463 3.29e-19 90.9
Msa0130160 MtrunA17_Chr6g0463941 61.957 92 34 1 117 208 54 144 2.05e-31 128
Msa0130160 MtrunA17_Chr6g0463941 45.652 92 48 2 114 204 460 550 9.03e-19 89.7
Msa0130160 MtrunA17_Chr2g0290511 27.193 228 116 8 307 492 114 333 1.79e-14 75.1
Msa0130160 MtrunA17_Chr2g0290511 39.785 93 54 2 113 204 257 348 2.07e-13 72.0
Msa0130160 MtrunA17_Chr7g0273941 42.268 97 45 3 118 204 381 476 1.60e-13 72.8
Msa0130160 MtrunA17_Chr1g0190851 45.122 82 42 2 110 189 410 490 5.08e-12 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0130160 AT5G03680.1 42.455 391 176 9 118 481 119 487 1.37e-85 275
Msa0130160 AT3G10000.1 39.071 366 191 12 40 399 15 354 4.61e-65 218
Msa0130160 AT3G10000.2 37.158 366 188 13 40 399 15 344 9.23e-57 196
Msa0130160 AT1G76890.2 31.696 448 215 12 114 488 37 466 9.92e-50 179
Msa0130160 AT1G76890.2 40.000 160 78 8 88 233 360 515 1.48e-19 92.4
Msa0130160 AT1G76880.1 54.701 117 46 2 97 213 46 155 8.16e-31 126
Msa0130160 AT1G76880.1 45.361 97 51 2 114 209 404 499 7.93e-18 87.0
Msa0130160 AT1G33240.1 44.318 88 47 2 118 204 435 521 2.95e-15 79.0
Msa0130160 AT1G33240.3 44.318 88 47 2 118 204 435 521 3.08e-15 79.0
Msa0130160 AT1G33240.2 44.318 88 47 2 118 204 396 482 3.89e-15 78.6
Msa0130160 AT5G28300.1 37.288 118 56 5 102 208 449 559 4.51e-13 72.0

Find 115 sgRNAs with CRISPR-Local

Find 163 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTGGCTCTTACTCTTATT+TGG 0.155961 1_3:+60258326 None:intergenic
TTGTTGTATCATTTGCTTCT+TGG 0.177243 1_3:+60258309 None:intergenic
TTATTTATATGTTTGGATTA+AGG 0.199931 1_3:-60257651 None:intergenic
TGCATTTCTTTCCACTTCTT+TGG 0.203428 1_3:+60259017 None:intergenic
AGGATAATGGCTGAGGAATT+TGG 0.204963 1_3:-60259043 Msa0130160:CDS
CCTTGTGGAATGAAATTTCT+AGG 0.236619 1_3:-60260060 Msa0130160:intron
GAACATCAAGTAAGAGAAAA+AGG 0.239292 1_3:-60258197 Msa0130160:CDS
AGGTGAAAGGATGGTTTATA+TGG 0.259177 1_3:-60258144 Msa0130160:CDS
TTTGGTTCTGAGCATGAAAA+AGG 0.260495 1_3:-60257942 Msa0130160:three_prime_UTR
GATATTTGGATGATGGAGTT+TGG 0.262012 1_3:-60258175 Msa0130160:CDS
AAACACAAGGCCTTGGTGTT+TGG 0.270837 1_3:-60258036 Msa0130160:CDS
AAATATATTATTTATATGTT+TGG 0.272764 1_3:-60257658 Msa0130160:three_prime_UTR
GAGCTGAGTGCAAGAAAATA+TGG 0.284773 1_3:-60258106 Msa0130160:CDS
AACACAAGGCCTTGGTGTTT+GGG 0.305639 1_3:-60258035 Msa0130160:CDS
TCTACTAGCTTTACCTTCCT+TGG 0.314459 1_3:+60258955 None:intergenic
GATCTTCTTGTTTAGGATAA+TGG 0.314988 1_3:-60259056 Msa0130160:intron
ATCATATTTATGAACTTTGT+AGG 0.329585 1_3:-60257736 Msa0130160:three_prime_UTR
TGGTTTATATGGGATTTAAT+AGG 0.331668 1_3:-60258133 Msa0130160:CDS
ATAGGGTCATAGGAATAATT+TGG 0.333734 1_3:+60260373 None:intergenic
ATCAAGAAATACATGAACTT+TGG 0.336932 1_3:-60258433 Msa0130160:CDS
GGATAATGGCTGAGGAATTT+GGG 0.344397 1_3:-60259042 Msa0130160:CDS
GGGTCATTGACTTGCAAGTT+TGG 0.355678 1_3:-60257921 Msa0130160:three_prime_UTR
GGTTTAGAAACTTCTGATAA+AGG 0.359679 1_3:-60260199 Msa0130160:CDS
AAAACATCACCAACTATCAT+AGG 0.369956 1_3:+60260355 None:intergenic
GAGAAGATGAGTGACCTATA+TGG 0.371968 1_3:-60260502 Msa0130160:exon
ATATTTGGATGATGGAGTTT+GGG 0.376772 1_3:-60258174 Msa0130160:CDS
GATGGAGTTTGGGATGAAAT+AGG 0.383462 1_3:-60258164 Msa0130160:CDS
TCAAGAAATACATGAACTTT+GGG 0.384682 1_3:-60258432 Msa0130160:CDS
GTAGCAACAAAGGAAGCATT+AGG 0.385971 1_3:+60258836 None:intergenic
GCAGCTATAGTGGTATTTGT+AGG 0.401800 1_3:+60260280 None:intergenic
GAAGTTGTAAGAAAACATAT+TGG 0.406391 1_3:-60258365 Msa0130160:CDS
TCCTATGACCCTATGATAGT+TGG 0.417353 1_3:-60260364 Msa0130160:CDS
AAGTTGTAAGAAAACATATT+GGG 0.418479 1_3:-60258364 Msa0130160:CDS
TAGCAACAAAGGAAGCATTA+GGG 0.434533 1_3:+60258837 None:intergenic
GAGGCATGAGAGTTTGTGTT+TGG 0.435521 1_3:+60258878 None:intergenic
GAACCACCACCTTGTTTGTA+TGG 0.437886 1_3:-60260418 Msa0130160:CDS
AGAAAGGGCAAAGTGGTCAT+AGG 0.438060 1_3:-60258631 Msa0130160:CDS
ATATTAGTATGTCTCTTAGA+AGG 0.438578 1_3:-60258079 Msa0130160:CDS
TATATGGGATTTAATAGGAA+TGG 0.452119 1_3:-60258128 Msa0130160:CDS
GATCCTCCGGTAGCCCATAT+AGG 0.456344 1_3:+60260488 None:intergenic
ACCAACTATCATAGGGTCAT+AGG 0.456713 1_3:+60260363 None:intergenic
TGCAGCAGCAGCAGCTATAG+TGG 0.457554 1_3:+60260270 None:intergenic
ATATTATCCACACTTTCAAA+AGG 0.458934 1_3:+60258275 None:intergenic
GCTTCTACTGCATTCTGTGT+AGG 0.459654 1_3:-60260220 Msa0130160:CDS
TATTCATAGATGGACAGAAA+TGG 0.466542 1_3:-60258255 Msa0130160:CDS
AGAACAAGAGATGGCATCAA+AGG 0.471241 1_3:-60258492 Msa0130160:CDS
AGAAGATGAGTGACCTATAT+GGG 0.473607 1_3:-60260501 Msa0130160:exon
TTTCAAACTTGATACAACTA+AGG 0.475487 1_3:-60258229 Msa0130160:CDS
ATTAACCAAGAAAATACTAA+TGG 0.483532 1_3:-60258794 Msa0130160:CDS
ATCAACCGCCGGAGATCCTC+CGG 0.484296 1_3:+60260475 None:intergenic
TTATTGAGACTCAAGATGCT+TGG 0.485535 1_3:-60258547 Msa0130160:CDS
ATGAAGTCGTCGAAGGAGAA+AGG 0.490837 1_3:-60258647 Msa0130160:CDS
AAACAAGGTGGTGGTTCTGC+TGG 0.491751 1_3:+60260424 None:intergenic
GGTGAAAGGATGGTTTATAT+GGG 0.497182 1_3:-60258143 Msa0130160:CDS
GGATGAAATAGGTGAAAGGA+TGG 0.499715 1_3:-60258153 Msa0130160:CDS
GTGTGGATAATATTCATAGA+TGG 0.500320 1_3:-60258265 Msa0130160:CDS
CTTGTTTAGGATAATGGCTG+AGG 0.502416 1_3:-60259050 Msa0130160:intron
ATAGGAGGGCAAGGAAAAGT+TGG 0.504848 1_3:-60258613 Msa0130160:CDS
ACTTCTGATAAAGGATGGCT+TGG 0.509259 1_3:-60260190 Msa0130160:CDS
TATTATCCACACTTTCAAAA+GGG 0.515340 1_3:+60258276 None:intergenic
TTGATTCATGTAACAACAGA+TGG 0.515630 1_3:-60260162 Msa0130160:CDS
TAGAAACTTCTGATAAAGGA+TGG 0.515925 1_3:-60260195 Msa0130160:CDS
CAGCTATAGTGGTATTTGTA+GGG 0.519351 1_3:+60260281 None:intergenic
TGAGACTCAAGATGCTTGGA+TGG 0.522893 1_3:-60258543 Msa0130160:CDS
AGACAGCTTGAAGCAATATG+TGG 0.533898 1_3:-60258905 Msa0130160:CDS
TGAAGTCGTCGAAGGAGAAA+GGG 0.536001 1_3:-60258646 Msa0130160:CDS
TGGCATCAAAGGAAGAAGAA+AGG 0.537348 1_3:-60258481 Msa0130160:CDS
TTAGACTTTGTGAATACTTG+TGG 0.538073 1_3:+60258747 None:intergenic
CTATTTAAACTTAAAACACA+TGG 0.546724 1_3:+60257859 None:intergenic
GGAAGAGAAAAGAGTCAATG+AGG 0.550829 1_3:-60258460 Msa0130160:CDS
AGAGAAAGAGTCTCTTGTCT+AGG 0.553492 1_3:+60260139 None:intergenic
ATGGGCTACCGGAGGATCTC+CGG 0.559641 1_3:-60260483 Msa0130160:CDS
AAACATCACCAACTATCATA+GGG 0.561437 1_3:+60260356 None:intergenic
TTGAGTTTGAGTAGCAACAA+AGG 0.562015 1_3:+60258826 None:intergenic
AAGGGCAAAGTGGTCATAGG+AGG 0.562963 1_3:-60258628 Msa0130160:CDS
GAGTGACCTATATGGGCTAC+CGG 0.564245 1_3:-60260494 Msa0130160:CDS
GGTATTTGTAGGGTTAACAG+TGG 0.564956 1_3:+60260291 None:intergenic
GGTAAAGCTAGTAGACAAGA+TGG 0.568681 1_3:-60258947 Msa0130160:CDS
CCTAGAAATTTCATTCCACA+AGG 0.573860 1_3:+60260060 None:intergenic
TCATAGGAATAATTTGGTGG+AGG 0.577820 1_3:+60260379 None:intergenic
GAATTTGGGTACCAAAGAAG+TGG 0.582190 1_3:-60259028 Msa0130160:CDS
CAGTGGAGGAATAGTCATAG+TGG 0.586600 1_3:+60260308 None:intergenic
TTGATGATGTTATCAACCGC+CGG 0.588660 1_3:+60260464 None:intergenic
AGGGCAAGGAAAAGTTGGAG+AGG 0.591096 1_3:-60258608 Msa0130160:CDS
ATTTGTAGGGTTAACAGTGG+AGG 0.594970 1_3:+60260294 None:intergenic
AGCTGAGTGCAAGAAAATAT+GGG 0.595363 1_3:-60258105 Msa0130160:CDS
TTTGGGATGAAATAGGTGAA+AGG 0.600563 1_3:-60258157 Msa0130160:CDS
TATTACAAGAAGACCAAGGA+AGG 0.601931 1_3:-60258968 Msa0130160:CDS
GGGTCATAGGAATAATTTGG+TGG 0.603660 1_3:+60260376 None:intergenic
AAGTTGGAGAGGTAAAGTTG+AGG 0.607935 1_3:-60258597 Msa0130160:CDS
ATAATCAGAAAGCACCCTTG+TGG 0.619380 1_3:-60260075 Msa0130160:CDS
AGGTGTGTAAGACTCTAGCT+AGG 0.621926 1_3:-60257882 Msa0130160:three_prime_UTR
AAAGTGGTCATAGGAGGGCA+AGG 0.623960 1_3:-60258622 Msa0130160:CDS
GGTGAGTGAAGCTACTACGA+GGG 0.624158 1_3:+60260329 None:intergenic
TGAACAAAGAGAACAAGAGA+TGG 0.625612 1_3:-60258501 Msa0130160:CDS
AGCAACAAAGGAAGCATTAG+GGG 0.629023 1_3:+60258838 None:intergenic
CTTAGAAGGACAGTGGACTG+TGG 0.637596 1_3:-60258065 Msa0130160:CDS
AAGAAGTGGAAAGAAATGCA+AGG 0.641850 1_3:-60259014 Msa0130160:CDS
AGGGCAAAGTGGTCATAGGA+GGG 0.643035 1_3:-60258627 Msa0130160:CDS
TTGGTTCATTGAAAATCACA+AGG 0.643246 1_3:-60257902 Msa0130160:three_prime_UTR
CCACCATACAAACAAGGTGG+TGG 0.656823 1_3:+60260415 None:intergenic
ACACACTATGAAGTCGTCGA+AGG 0.660577 1_3:-60258654 Msa0130160:CDS
TATGTCTCTTAGAAGGACAG+TGG 0.662720 1_3:-60258072 Msa0130160:CDS
CTCATCTGAAAACAACGATG+AGG 0.668787 1_3:-60258693 Msa0130160:CDS
CCACCACCTTGTTTGTATGG+TGG 0.671674 1_3:-60260415 Msa0130160:CDS
AGCTTAAGTCCCAAACACCA+AGG 0.671681 1_3:+60258026 None:intergenic
AACTTGATACAACTAAGGAG+TGG 0.671997 1_3:-60258224 Msa0130160:CDS
TCGAAGGAGAAAGGGCAAAG+TGG 0.682624 1_3:-60258638 Msa0130160:CDS
GCAACACCACCATACAAACA+AGG 0.686159 1_3:+60260409 None:intergenic
GGCTACCGGAGGATCTCCGG+CGG 0.699131 1_3:-60260480 Msa0130160:CDS
TGACCTATATGGGCTACCGG+AGG 0.701402 1_3:-60260491 Msa0130160:CDS
CAAGTATTACAAGAAGACCA+AGG 0.709647 1_3:-60258972 Msa0130160:CDS
CTAGAAATTTCATTCCACAA+GGG 0.711484 1_3:+60260061 None:intergenic
TGGTGAGTGAAGCTACTACG+AGG 0.746302 1_3:+60260328 None:intergenic
ACACCACCATACAAACAAGG+TGG 0.760464 1_3:+60260412 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATTTCACTTTTTTTTTT+TGG - chr1_3:60258198-60258217 Msa0130160:CDS 10.0%
!! TTAAATTTAAATTGTAGTTA+CGG - chr1_3:60258836-60258855 Msa0130160:CDS 10.0%
!! ATCATAATAAAAAAAACACA+TGG + chr1_3:60258311-60258330 None:intergenic 15.0%
!! TTAGTGTCTTAATTAATTAC+TGG - chr1_3:60258163-60258182 Msa0130160:CDS 20.0%
!! GACAAAAAAGAAAATTATCA+TGG + chr1_3:60258263-60258282 None:intergenic 20.0%
!!! TTTTGTTTGAAAATATCATG+TGG - chr1_3:60258670-60258689 Msa0130160:CDS 20.0%
!! ATTAACCAAGAAAATACTAA+TGG - chr1_3:60259400-60259419 Msa0130160:intron 20.0%
!! AAGTTGTAAGAAAACATATT+GGG - chr1_3:60259830-60259849 Msa0130160:intron 20.0%
!!! TTTTACATTAATTGTGTCAA+AGG + chr1_3:60260209-60260228 None:intergenic 20.0%
!! CTATTTAAACTTAAAACACA+TGG + chr1_3:60260338-60260357 None:intergenic 20.0%
!!! CATGTGTTTTAAGTTTAAAT+AGG - chr1_3:60260336-60260355 Msa0130160:CDS 20.0%
!! ATCATATTTATGAACTTTGT+AGG - chr1_3:60260458-60260477 Msa0130160:CDS 20.0%
! CAATGAATGATTTAGTATCA+TGG + chr1_3:60258405-60258424 None:intergenic 25.0%
! AGTATATACAAAACAAAGAC+AGG + chr1_3:60258461-60258480 None:intergenic 25.0%
!! ATTTTCTTGGTTAATTGTGA+AGG + chr1_3:60259395-60259414 None:intergenic 25.0%
! ATCAAGAAATACATGAACTT+TGG - chr1_3:60259761-60259780 Msa0130160:intron 25.0%
! TCAAGAAATACATGAACTTT+GGG - chr1_3:60259762-60259781 Msa0130160:intron 25.0%
! GAAGTTGTAAGAAAACATAT+TGG - chr1_3:60259829-60259848 Msa0130160:intron 25.0%
! TATTATCCACACTTTCAAAA+GGG + chr1_3:60259921-60259940 None:intergenic 25.0%
! ATATTATCCACACTTTCAAA+AGG + chr1_3:60259922-60259941 None:intergenic 25.0%
! TTTCAAACTTGATACAACTA+AGG - chr1_3:60259965-60259984 Msa0130160:intron 25.0%
! AGTAAGAGAAAAAGGATATT+TGG - chr1_3:60260005-60260024 Msa0130160:intron 25.0%
! TGGTTTATATGGGATTTAAT+AGG - chr1_3:60260061-60260080 Msa0130160:CDS 25.0%
! TATATGGGATTTAATAGGAA+TGG - chr1_3:60260066-60260085 Msa0130160:CDS 25.0%
! ATATTAGTATGTCTCTTAGA+AGG - chr1_3:60260115-60260134 Msa0130160:CDS 25.0%
ATAGGGTCATAGGAATAATT+TGG + chr1_3:60257824-60257843 None:intergenic 30.0%
AAACATCACCAACTATCATA+GGG + chr1_3:60257841-60257860 None:intergenic 30.0%
AAAACATCACCAACTATCAT+AGG + chr1_3:60257842-60257861 None:intergenic 30.0%
GGTTTAGAAACTTCTGATAA+AGG - chr1_3:60257995-60258014 Msa0130160:exon 30.0%
TAGAAACTTCTGATAAAGGA+TGG - chr1_3:60257999-60258018 Msa0130160:exon 30.0%
TTGATTCATGTAACAACAGA+TGG - chr1_3:60258032-60258051 Msa0130160:CDS 30.0%
CTAGAAATTTCATTCCACAA+GGG + chr1_3:60258136-60258155 None:intergenic 30.0%
!!! CTGTCATTTTCAATCACAAA+AGG + chr1_3:60258232-60258251 None:intergenic 30.0%
! CTTTTGTGATTGAAAATGAC+AGG - chr1_3:60258230-60258249 Msa0130160:CDS 30.0%
GATACTAAATCATTCATTGC+AGG - chr1_3:60258406-60258425 Msa0130160:CDS 30.0%
! TGTTAGTGTTTTGTACTGAA+AGG + chr1_3:60258612-60258631 None:intergenic 30.0%
AAATCAAGTGATACTGTGAT+AGG + chr1_3:60258646-60258665 None:intergenic 30.0%
TTGAAAATATCATGTGGTCT+TGG - chr1_3:60258676-60258695 Msa0130160:CDS 30.0%
TGATTGTAATTGAAACCTTG+GGG + chr1_3:60258728-60258747 None:intergenic 30.0%
GTGATTGTAATTGAAACCTT+GGG + chr1_3:60258729-60258748 None:intergenic 30.0%
TGTGATTGTAATTGAAACCT+TGG + chr1_3:60258730-60258749 None:intergenic 30.0%
! GATCTTCTTGTTTAGGATAA+TGG - chr1_3:60259138-60259157 Msa0130160:intron 30.0%
!! GGTAATTTTGTCTAAAGTTG+AGG + chr1_3:60259338-60259357 None:intergenic 30.0%
! CAACACCATTAGTATTTTCT+TGG + chr1_3:60259408-60259427 None:intergenic 30.0%
TTAGACTTTGTGAATACTTG+TGG + chr1_3:60259450-60259469 None:intergenic 30.0%
TTGTTGTATCATTTGCTTCT+TGG + chr1_3:60259888-60259907 None:intergenic 30.0%
GTGTGGATAATATTCATAGA+TGG - chr1_3:60259929-60259948 Msa0130160:intron 30.0%
TATTCATAGATGGACAGAAA+TGG - chr1_3:60259939-60259958 Msa0130160:intron 30.0%
GAACATCAAGTAAGAGAAAA+AGG - chr1_3:60259997-60260016 Msa0130160:intron 30.0%
GAAAAAGGATATTTGGATGA+TGG - chr1_3:60260012-60260031 Msa0130160:intron 30.0%
!! ATATTTGGATGATGGAGTTT+GGG - chr1_3:60260020-60260039 Msa0130160:intron 30.0%
TTGGTTCATTGAAAATCACA+AGG - chr1_3:60260292-60260311 Msa0130160:CDS 30.0%
AGAAGATGAGTGACCTATAT+GGG - chr1_3:60257693-60257712 Msa0130160:three_prime_UTR 35.0%
! TCATAGGAATAATTTGGTGG+AGG + chr1_3:60257818-60257837 None:intergenic 35.0%
CAGCTATAGTGGTATTTGTA+GGG + chr1_3:60257916-60257935 None:intergenic 35.0%
CCTAGAAATTTCATTCCACA+AGG + chr1_3:60258137-60258156 None:intergenic 35.0%
CCTTGTGGAATGAAATTTCT+AGG - chr1_3:60258134-60258153 Msa0130160:CDS 35.0%
TTCATTGCAGGTGCAAATTA+AGG - chr1_3:60258418-60258437 Msa0130160:CDS 35.0%
! TAGTACAACTTTTTACCCCA+AGG - chr1_3:60258710-60258729 Msa0130160:CDS 35.0%
TATCCCACAAATCTTTGCAT+TGG - chr1_3:60258774-60258793 Msa0130160:CDS 35.0%
GCACAAAGCTGTAATAAATG+TGG - chr1_3:60258945-60258964 Msa0130160:CDS 35.0%
! GCTTTGTGATCTTCTTGTTT+AGG - chr1_3:60259131-60259150 Msa0130160:intron 35.0%
TGCATTTCTTTCCACTTCTT+TGG + chr1_3:60259180-60259199 None:intergenic 35.0%
AAGAAGTGGAAAGAAATGCA+AGG - chr1_3:60259180-60259199 Msa0130160:intron 35.0%
CAAGTATTACAAGAAGACCA+AGG - chr1_3:60259222-60259241 Msa0130160:intron 35.0%
TATTACAAGAAGACCAAGGA+AGG - chr1_3:60259226-60259245 Msa0130160:intron 35.0%
!! TAGCAACAAAGGAAGCATTA+GGG + chr1_3:60259360-60259379 None:intergenic 35.0%
TTGAGTTTGAGTAGCAACAA+AGG + chr1_3:60259371-60259390 None:intergenic 35.0%
TTATTGAGACTCAAGATGCT+TGG - chr1_3:60259647-60259666 Msa0130160:intron 35.0%
TGAACAAAGAGAACAAGAGA+TGG - chr1_3:60259693-60259712 Msa0130160:intron 35.0%
TTTGGGCTAAAGAAAAAGCA+TGG - chr1_3:60259779-60259798 Msa0130160:intron 35.0%
! TTGGGCTAAAGAAAAAGCAT+GGG - chr1_3:60259780-60259799 Msa0130160:intron 35.0%
! TCTTGGCTCTTACTCTTATT+TGG + chr1_3:60259871-60259890 None:intergenic 35.0%
!! TGAATACCCTTTTGAAAGTG+TGG - chr1_3:60259912-60259931 Msa0130160:intron 35.0%
AACTTGATACAACTAAGGAG+TGG - chr1_3:60259970-60259989 Msa0130160:intron 35.0%
!! GATATTTGGATGATGGAGTT+TGG - chr1_3:60260019-60260038 Msa0130160:intron 35.0%
! TTTGGGATGAAATAGGTGAA+AGG - chr1_3:60260037-60260056 Msa0130160:intron 35.0%
AGGTGAAAGGATGGTTTATA+TGG - chr1_3:60260050-60260069 Msa0130160:intron 35.0%
GGTGAAAGGATGGTTTATAT+GGG - chr1_3:60260051-60260070 Msa0130160:intron 35.0%
AGCTGAGTGCAAGAAAATAT+GGG - chr1_3:60260089-60260108 Msa0130160:CDS 35.0%
!!! GCACATGAATCAGAGTTTTT+TGG - chr1_3:60260234-60260253 Msa0130160:CDS 35.0%
!! TTTGGTTCTGAGCATGAAAA+AGG - chr1_3:60260252-60260271 Msa0130160:CDS 35.0%
!! TTGGTTCTGAGCATGAAAAA+GGG - chr1_3:60260253-60260272 Msa0130160:CDS 35.0%
GAGAAGATGAGTGACCTATA+TGG - chr1_3:60257692-60257711 Msa0130160:three_prime_UTR 40.0%
!! TTGATGATGTTATCAACCGC+CGG + chr1_3:60257733-60257752 None:intergenic 40.0%
GGGTCATAGGAATAATTTGG+TGG + chr1_3:60257821-60257840 None:intergenic 40.0%
TCCTATGACCCTATGATAGT+TGG - chr1_3:60257830-60257849 Msa0130160:three_prime_UTR 40.0%
ACCAACTATCATAGGGTCAT+AGG + chr1_3:60257834-60257853 None:intergenic 40.0%
ATTTGTAGGGTTAACAGTGG+AGG + chr1_3:60257903-60257922 None:intergenic 40.0%
GGTATTTGTAGGGTTAACAG+TGG + chr1_3:60257906-60257925 None:intergenic 40.0%
GCAGCTATAGTGGTATTTGT+AGG + chr1_3:60257917-60257936 None:intergenic 40.0%
ACTTCTGATAAAGGATGGCT+TGG - chr1_3:60258004-60258023 Msa0130160:CDS 40.0%
AGAGAAAGAGTCTCTTGTCT+AGG + chr1_3:60258058-60258077 None:intergenic 40.0%
!! ATAATCAGAAAGCACCCTTG+TGG - chr1_3:60258119-60258138 Msa0130160:CDS 40.0%
GTGACTATACTTACTTGCAC+TGG - chr1_3:60258372-60258391 Msa0130160:CDS 40.0%
GCTGAGCGAATTAGGTTAAT+AGG + chr1_3:60258489-60258508 None:intergenic 40.0%
CCTCCAATGCAAAGATTTGT+GGG + chr1_3:60258780-60258799 None:intergenic 40.0%
TCCTCCAATGCAAAGATTTG+TGG + chr1_3:60258781-60258800 None:intergenic 40.0%
TTTGCATTGGAGGAGATCAA+AGG - chr1_3:60258787-60258806 Msa0130160:CDS 40.0%
TTGCATTGGAGGAGATCAAA+GGG - chr1_3:60258788-60258807 Msa0130160:CDS 40.0%
! CTTGTTTAGGATAATGGCTG+AGG - chr1_3:60259144-60259163 Msa0130160:intron 40.0%
! AGGATAATGGCTGAGGAATT+TGG - chr1_3:60259151-60259170 Msa0130160:intron 40.0%
! GGATAATGGCTGAGGAATTT+GGG - chr1_3:60259152-60259171 Msa0130160:intron 40.0%
GAATTTGGGTACCAAAGAAG+TGG - chr1_3:60259166-60259185 Msa0130160:intron 40.0%
!! TCTACTAGCTTTACCTTCCT+TGG + chr1_3:60259242-60259261 None:intergenic 40.0%
GGTAAAGCTAGTAGACAAGA+TGG - chr1_3:60259247-60259266 Msa0130160:intron 40.0%
AGACAGCTTGAAGCAATATG+TGG - chr1_3:60259289-60259308 Msa0130160:intron 40.0%
!! AGCAACAAAGGAAGCATTAG+GGG + chr1_3:60259359-60259378 None:intergenic 40.0%
!! GTAGCAACAAAGGAAGCATT+AGG + chr1_3:60259361-60259380 None:intergenic 40.0%
!! CTCATCGTTGTTTTCAGATG+AGG + chr1_3:60259503-60259522 None:intergenic 40.0%
CTCATCTGAAAACAACGATG+AGG - chr1_3:60259501-60259520 Msa0130160:intron 40.0%
AAGTTGGAGAGGTAAAGTTG+AGG - chr1_3:60259597-60259616 Msa0130160:intron 40.0%
AGAACAAGAGATGGCATCAA+AGG - chr1_3:60259702-60259721 Msa0130160:intron 40.0%
TGGCATCAAAGGAAGAAGAA+AGG - chr1_3:60259713-60259732 Msa0130160:intron 40.0%
GGAAGAGAAAAGAGTCAATG+AGG - chr1_3:60259734-60259753 Msa0130160:intron 40.0%
! GATGGAGTTTGGGATGAAAT+AGG - chr1_3:60260030-60260049 Msa0130160:intron 40.0%
GGATGAAATAGGTGAAAGGA+TGG - chr1_3:60260041-60260060 Msa0130160:intron 40.0%
GAGCTGAGTGCAAGAAAATA+TGG - chr1_3:60260088-60260107 Msa0130160:CDS 40.0%
TATGTCTCTTAGAAGGACAG+TGG - chr1_3:60260122-60260141 Msa0130160:CDS 40.0%
ACTGTGGAGTCAAAAACACA+AGG - chr1_3:60260145-60260164 Msa0130160:CDS 40.0%
! GAACCACCACCTTGTTTGTA+TGG - chr1_3:60257776-60257795 Msa0130160:three_prime_UTR 45.0%
ACACCACCATACAAACAAGG+TGG + chr1_3:60257785-60257804 None:intergenic 45.0%
GCAACACCACCATACAAACA+AGG + chr1_3:60257788-60257807 None:intergenic 45.0%
CAGTGGAGGAATAGTCATAG+TGG + chr1_3:60257889-60257908 None:intergenic 45.0%
GCTTCTACTGCATTCTGTGT+AGG - chr1_3:60257974-60257993 Msa0130160:three_prime_UTR 45.0%
AAACTCCAATAGCCCTAGCT+AGG + chr1_3:60258514-60258533 None:intergenic 45.0%
CCCACAAATCTTTGCATTGG+AGG - chr1_3:60258777-60258796 Msa0130160:CDS 45.0%
GAGGCATGAGAGTTTGTGTT+TGG + chr1_3:60259319-60259338 None:intergenic 45.0%
ACACACTATGAAGTCGTCGA+AGG - chr1_3:60259540-60259559 Msa0130160:intron 45.0%
ATGAAGTCGTCGAAGGAGAA+AGG - chr1_3:60259547-60259566 Msa0130160:intron 45.0%
TGAAGTCGTCGAAGGAGAAA+GGG - chr1_3:60259548-60259567 Msa0130160:intron 45.0%
AGAAAGGGCAAAGTGGTCAT+AGG - chr1_3:60259563-60259582 Msa0130160:intron 45.0%
ATAGGAGGGCAAGGAAAAGT+TGG - chr1_3:60259581-60259600 Msa0130160:intron 45.0%
TGAGACTCAAGATGCTTGGA+TGG - chr1_3:60259651-60259670 Msa0130160:intron 45.0%
GAGTCAAAAACACAAGGCCT+TGG - chr1_3:60260151-60260170 Msa0130160:CDS 45.0%
!! AAACACAAGGCCTTGGTGTT+TGG - chr1_3:60260158-60260177 Msa0130160:CDS 45.0%
!! AACACAAGGCCTTGGTGTTT+GGG - chr1_3:60260159-60260178 Msa0130160:CDS 45.0%
AGCTTAAGTCCCAAACACCA+AGG + chr1_3:60260171-60260190 None:intergenic 45.0%
!! GGGTCATTGACTTGCAAGTT+TGG - chr1_3:60260273-60260292 Msa0130160:CDS 45.0%
AGGTGTGTAAGACTCTAGCT+AGG - chr1_3:60260312-60260331 Msa0130160:CDS 45.0%
!! AAATATATTATTTATATGTT+TGG - chr1_3:60260536-60260555 Msa0130160:five_prime_UTR 5.0%
! GAGTGACCTATATGGGCTAC+CGG - chr1_3:60257700-60257719 Msa0130160:three_prime_UTR 50.0%
! AAACAAGGTGGTGGTTCTGC+TGG + chr1_3:60257773-60257792 None:intergenic 50.0%
CCACCATACAAACAAGGTGG+TGG + chr1_3:60257782-60257801 None:intergenic 50.0%
! CCACCACCTTGTTTGTATGG+TGG - chr1_3:60257779-60257798 Msa0130160:three_prime_UTR 50.0%
GGTGAGTGAAGCTACTACGA+GGG + chr1_3:60257868-60257887 None:intergenic 50.0%
TGGTGAGTGAAGCTACTACG+AGG + chr1_3:60257869-60257888 None:intergenic 50.0%
GCTAGGTAGCTGAGCGAATT+AGG + chr1_3:60258497-60258516 None:intergenic 50.0%
ATTCGCTCAGCTACCTAGCT+AGG - chr1_3:60258498-60258517 Msa0130160:CDS 50.0%
TTCGCTCAGCTACCTAGCTA+GGG - chr1_3:60258499-60258518 Msa0130160:CDS 50.0%
AGCTACCTAGCTAGGGCTAT+TGG - chr1_3:60258506-60258525 Msa0130160:CDS 50.0%
TCGAAGGAGAAAGGGCAAAG+TGG - chr1_3:60259556-60259575 Msa0130160:intron 50.0%
AAGGGCAAAGTGGTCATAGG+AGG - chr1_3:60259566-60259585 Msa0130160:intron 50.0%
AGGGCAAAGTGGTCATAGGA+GGG - chr1_3:60259567-60259586 Msa0130160:intron 50.0%
AAAGTGGTCATAGGAGGGCA+AGG - chr1_3:60259572-60259591 Msa0130160:intron 50.0%
AGGGCAAGGAAAAGTTGGAG+AGG - chr1_3:60259586-60259605 Msa0130160:intron 50.0%
!! CTTAGAAGGACAGTGGACTG+TGG - chr1_3:60260129-60260148 Msa0130160:CDS 50.0%
! TGACCTATATGGGCTACCGG+AGG - chr1_3:60257703-60257722 Msa0130160:three_prime_UTR 55.0%
GATCCTCCGGTAGCCCATAT+AGG + chr1_3:60257709-60257728 None:intergenic 55.0%
TGCAGCAGCAGCAGCTATAG+TGG + chr1_3:60257927-60257946 None:intergenic 55.0%
! ATGGGCTACCGGAGGATCTC+CGG - chr1_3:60257711-60257730 Msa0130160:three_prime_UTR 60.0%
ATCAACCGCCGGAGATCCTC+CGG + chr1_3:60257722-60257741 None:intergenic 60.0%
GGCTACCGGAGGATCTCCGG+CGG - chr1_3:60257714-60257733 Msa0130160:three_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 60257653 60260563 60257653 ID=Msa0130160;Name=Msa0130160
chr1_3 mRNA 60257653 60260563 60257653 ID=Msa0130160-mRNA-1;Parent=Msa0130160;Name=Msa0130160-mRNA-1;_AED=0.00;_eAED=0.00;_QI=45|1|1|1|1|1|2|351|505
chr1_3 exon 60257653 60259063 60257653 ID=Msa0130160-mRNA-1:exon:19836;Parent=Msa0130160-mRNA-1
chr1_3 exon 60260061 60260563 60260061 ID=Msa0130160-mRNA-1:exon:19835;Parent=Msa0130160-mRNA-1
chr1_3 five_prime_UTR 60260519 60260563 60260519 ID=Msa0130160-mRNA-1:five_prime_utr;Parent=Msa0130160-mRNA-1
chr1_3 CDS 60260061 60260518 60260061 ID=Msa0130160-mRNA-1:cds;Parent=Msa0130160-mRNA-1
chr1_3 CDS 60258004 60259063 60258004 ID=Msa0130160-mRNA-1:cds;Parent=Msa0130160-mRNA-1
chr1_3 three_prime_UTR 60257653 60258003 60257653 ID=Msa0130160-mRNA-1:three_prime_utr;Parent=Msa0130160-mRNA-1
Gene Sequence

>Msa0130160

ATGAGTGACCTATATGGGCTACCGGAGGATCTCCGGCGGTTGATAACATCATCAAGAAACACTACACATCTACCAGCAGAACCACCACCTTGTTTGTATGGTGGTGTTGCTACTTTTCCTCCACCAAATTATTCCTATGACCCTATGATAGTTGGTGATGTTTTCTACCCTCGTAGTAGCTTCACTCACCACTATGACTATTCCTCCACTGTTAACCCTACAAATACCACTATAGCTGCTGCTGCTGCAGCAGCTTCTAATTCAGATCATGCTACTGCTTCTACTGCATTCTGTGTAGGTTTAGAAACTTCTGATAAAGGATGGCTTGGTAACTTTGATTCATGTAACAACAGATGGCCTAGACAAGAGACTCTTTCTCTTCTTGAAATCAGATCTCGTCTTGATTCTAAGTTCAGAGAGAATAATCAGAAAGCACCCTTGTGGAATGAAATTTCTAGGATAATGGCTGAGGAATTTGGGTACCAAAGAAGTGGAAAGAAATGCAAGGAAAAGTTTGAGAATTTGTACAAGTATTACAAGAAGACCAAGGAAGGTAAAGCTAGTAGACAAGATGGTAAGCACTATAGATTTTTCAGACAGCTTGAAGCAATATGTGGAGAACCAAACACAAACTCTCATGCCTCAACTTTAGACAAAATTACCCCTAATGCTTCCTTTGTTGCTACTCAAACTCAAACCTTCACAATTAACCAAGAAAATACTAATGGTGTTGATCACAATTTGATCAATAACCACAAGTATTCACAAAGTCTAAGTTTCTCGAACTCGTCTGAATTTGAGACATCCTCATCTGAAAACAACGATGAGGATCTCTCAACTATTGCACACACTATGAAGTCGTCGAAGGAGAAAGGGCAAAGTGGTCATAGGAGGGCAAGGAAAAGTTGGAGAGGTAAAGTTGAGGAGATTGTAGATTCTCACATGAAAAATATTATTGAGACTCAAGATGCTTGGATGGAGAGAATGTTGAGTGTTGTTGAACAAAGAGAACAAGAGATGGCATCAAAGGAAGAAGAAAGGAAGAGAAAAGAGTCAATGAGGTTTGATCAAGAAATACATGAACTTTGGGCTAAAGAAAAAGCATGGGTTGAAGCAAGAGATTCTGCATTGTTAGAAGTTGTAAGAAAACATATTGGGATACAACTTGAAGCACCAAATAAGAGTAAGAGCCAAGAAGCAAATGATACAACAAATTATGAATACCCTTTTGAAAGTGTGGATAATATTCATAGATGGACAGAAATGGAAATTTCAAACTTGATACAACTAAGGAGTGGTTTTGAACATCAAGTAAGAGAAAAAGGATATTTGGATGATGGAGTTTGGGATGAAATAGGTGAAAGGATGGTTTATATGGGATTTAATAGGAATGGAGCTGAGTGCAAGAAAATATGGGATGATATTAGTATGTCTCTTAGAAGGACAGTGGACTGTGGAGTCAAAAACACAAGGCCTTGGTGTTTGGGACTTAAGCTGACAGATGATGACGACCTTTGA
Protein sequence

>Msa0130160

MSDLYGLPEDLRRLITSSRNTTHLPAEPPPCLYGGVATFPPPNYSYDPMIVGDVFYPRSSFTHHYDYSSTVNPTNTTIAAAAAAASNSDHATASTAFCVGLETSDKGWLGNFDSCNNRWPRQETLSLLEIRSRLDSKFRENNQKAPLWNEISRIMAEEFGYQRSGKKCKEKFENLYKYYKKTKEGKASRQDGKHYRFFRQLEAICGEPNTNSHASTLDKITPNASFVATQTQTFTINQENTNGVDHNLINNHKYSQSLSFSNSSEFETSSSENNDEDLSTIAHTMKSSKEKGQSGHRRARKSWRGKVEEIVDSHMKNIIETQDAWMERMLSVVEQREQEMASKEEERKRKESMRFDQEIHELWAKEKAWVEARDSALLEVVRKHIGIQLEAPNKSKSQEANDTTNYEYPFESVDNIHRWTEMEISNLIQLRSGFEHQVREKGYLDDGVWDEIGERMVYMGFNRNGAECKKIWDDISMSLRRTVDCGVKNTRPWCLGLKLTDDDDL*