AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0038820

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0038820 MtrunA17_Chr7g0252101 63.723 419 129 12 8 416 27 432 7.97e-171 485
Msa0038820 MtrunA17_Chr3g0093911 68.451 355 98 9 71 416 1 350 4.54e-156 444
Msa0038820 MtrunA17_Chr1g0207351 67.324 355 99 9 75 416 16 366 5.00e-152 434
Msa0038820 MtrunA17_Chr4g0075971 57.920 423 161 8 7 416 184 602 3.84e-148 433
Msa0038820 MtrunA17_Chr3g0077801 57.507 353 136 6 71 413 1 349 1.91e-129 377
Msa0038820 MtrunA17_Chr6g0488101 49.315 438 184 11 8 416 27 455 2.41e-127 375
Msa0038820 MtrunA17_Chr6g0488271 50.000 424 175 10 21 416 1 415 7.33e-127 372
Msa0038820 MtrunA17_Chr3g0077901 56.303 357 146 5 67 415 17 371 2.52e-124 364
Msa0038820 MtrunA17_Chr3g0077851 56.677 337 136 5 87 415 1 335 1.30e-117 346
Msa0038820 MtrunA17_Chr1g0209051 66.418 268 81 5 156 415 4 270 4.75e-117 342
Msa0038820 MtrunA17_Chr7g0243331 56.034 348 143 7 76 416 6 350 6.41e-114 337
Msa0038820 MtrunA17_Chr3g0112311 53.611 360 146 8 71 414 1 355 1.70e-111 331
Msa0038820 MtrunA17_Chr6g0452641 49.544 329 140 7 108 416 18 340 4.11e-85 263
Msa0038820 MtrunA17_Chr3g0122991 46.769 325 107 6 73 383 2 274 1.43e-82 254
Msa0038820 MtrunA17_Chr3g0123471 52.453 265 110 7 153 408 2 259 1.37e-77 241
Msa0038820 MtrunA17_Chr1g0202841 66.111 180 50 6 247 416 1 179 2.90e-69 216
Msa0038820 MtrunA17_Chr7g0243391 48.611 216 107 3 67 282 14 225 1.51e-55 183
Msa0038820 MtrunA17_Chr4g0075861 55.208 192 78 3 7 192 184 373 1.38e-52 180
Msa0038820 MtrunA17_Chr1g0196231 64.865 148 46 4 68 214 224 366 1.36e-49 172
Msa0038820 MtrunA17_Chr1g0187941 62.222 135 44 3 250 377 3 137 2.67e-45 153
Msa0038820 MtrunA17_Chr6g0486591 51.875 160 58 5 275 416 1 159 4.22e-42 145
Msa0038820 MtrunA17_Chr1g0206951 59.200 125 49 2 74 197 2 125 5.89e-40 139
Msa0038820 MtrunA17_Chr1g0209191 67.347 98 22 2 328 415 1 98 7.16e-37 130
Msa0038820 MtrunA17_Chr1g0196241 73.864 88 19 2 317 400 1 88 9.69e-35 124
Msa0038820 MtrunA17_Chr6g0459061 27.003 337 216 10 79 401 18 338 1.74e-26 109
Msa0038820 MtrunA17_Chr1g0209061 51.613 124 28 5 1 110 2 107 7.14e-26 100
Msa0038820 MtrunA17_Chr7g0238781 40.741 135 77 2 103 237 27 158 9.70e-24 96.7
Msa0038820 MtrunA17_Chr1g0206781 53.571 56 26 0 7 62 23 78 3.31e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 90 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTTCTTTATTCTTGTAAA+TGG 0.131332 1_1:+68285601 None:intergenic
TCCTACTCCTGTCAAATTAT+TGG 0.135605 1_1:+68284844 None:intergenic
TATGAAATTTGAAGAATTAA+TGG 0.141021 1_1:+68285781 None:intergenic
CTCTCATATCCCCACCTATT+TGG 0.159328 1_1:+68285088 None:intergenic
CTGCCTCGTCATGGTAGTTC+TGG 0.199501 1_1:+68287418 None:intergenic
CCTTTATGTAGTGTTTATTC+AGG 0.216363 1_1:-68285040 Msa0038820:CDS
TTTGAAGAATTAATGGTTCT+TGG 0.256322 1_1:+68285788 None:intergenic
CAAATTATTGGCAAACAAAA+AGG 0.284650 1_1:+68284856 None:intergenic
CTCCAGTTTGACATAATTCA+TGG 0.299341 1_1:+68285171 None:intergenic
TCTCATATCCCCACCTATTT+GGG 0.303333 1_1:+68285089 None:intergenic
GAAGCCGAGTCTTATCATTA+TGG 0.307560 1_1:-68285559 Msa0038820:CDS
ATGCATTGAGCAACCCAAAT+AGG 0.308917 1_1:-68285102 Msa0038820:CDS
AATTTACTGGAGAAATTTCT+TGG 0.311736 1_1:-68285815 Msa0038820:CDS
TGGATTACTTATTCGTCAAT+AGG 0.317482 1_1:-68285430 Msa0038820:CDS
GGTCGACAAAATCTAGTGTT+TGG 0.335860 1_1:+68285884 None:intergenic
CCTAAGGGAAAATGTTTCAT+TGG 0.345717 1_1:+68285276 None:intergenic
GGGGAGGGTGGTTGACTTTG+TGG 0.347817 1_1:-68285398 Msa0038820:CDS
TTGAAGAATTAATGGTTCTT+GGG 0.369399 1_1:+68285789 None:intergenic
AACCATGAATTATGTCAAAC+TGG 0.388496 1_1:-68285173 Msa0038820:CDS
TGCCTCGTCATGGTAGTTCT+GGG 0.393485 1_1:+68287419 None:intergenic
AGAGAACCGTGAACTTCCTT+TGG 0.394473 1_1:-68285926 Msa0038820:CDS
ATTTAAGGTTCGACGAAGTA+AGG 0.397434 1_1:-68285219 Msa0038820:CDS
AGTTTGATTTGATGTTCCTT+TGG 0.399942 1_1:+68287261 None:intergenic
CAAGTTCCTTCATGTTGGAT+TGG 0.401543 1_1:-68284970 Msa0038820:CDS
TTATGTCAAACTGGAGACTT+TGG 0.403683 1_1:-68285164 Msa0038820:CDS
ACACACCGGCAAATTGAAAA+AGG 0.404330 1_1:+68285863 None:intergenic
CTCTGAGGAATTTATCTTGG+TGG 0.409746 1_1:-68285506 Msa0038820:CDS
GTTGTGATCTAAAGGGAAAA+AGG 0.417556 1_1:-68285962 Msa0038820:intron
TTGCCGGTGTGTGCAAGAAT+TGG 0.418875 1_1:-68285852 Msa0038820:CDS
TTCGTCAATAGGAAATGAAC+GGG 0.420738 1_1:-68285419 Msa0038820:CDS
ATGCTCTGAGGAATTTATCT+TGG 0.430555 1_1:-68285509 Msa0038820:CDS
CTAAGGGAAAATGTTTCATT+GGG 0.435162 1_1:+68285277 None:intergenic
CCTGAATAAACACTACATAA+AGG 0.437679 1_1:+68285040 None:intergenic
GTCGACAAAATCTAGTGTTT+GGG 0.443544 1_1:+68285885 None:intergenic
TGATTTGATGTTCCTTTGGC+AGG 0.443659 1_1:+68287265 None:intergenic
TGTGTATGAATTGAATATGT+TGG 0.446075 1_1:-68284694 Msa0038820:three_prime_UTR
TTGTTTGCCAATAATTTGAC+AGG 0.461977 1_1:-68284851 Msa0038820:three_prime_UTR
AAGGAACTTGCTCCTTGAGT+TGG 0.466623 1_1:+68284983 None:intergenic
ATGATAAGACTCGGCTTCAA+AGG 0.473874 1_1:+68285564 None:intergenic
CATCACAATTAACTAACCTA+AGG 0.476886 1_1:+68285260 None:intergenic
GAGTACCCCTATGTTGGCCT+TGG 0.476948 1_1:+68285345 None:intergenic
TATGTCAAACTGGAGACTTT+GGG 0.481376 1_1:-68285163 Msa0038820:CDS
CTTTATGTAGTGTTTATTCA+GGG 0.488519 1_1:-68285039 Msa0038820:CDS
TGATAAGACTCGGCTTCAAA+GGG 0.489177 1_1:+68285565 None:intergenic
TCCATTTACAAGAATAAAGA+AGG 0.500617 1_1:-68285602 Msa0038820:CDS
ATCAATCTCGAAATTGTGGT+TGG 0.504810 1_1:-68285450 Msa0038820:CDS
AGGAGCAAGTTCCTTCATGT+TGG 0.509044 1_1:-68284975 Msa0038820:CDS
AGAGTCAAGGAGCTGCTTGA+AGG 0.509677 1_1:-68287321 Msa0038820:CDS
TTGGTCGGGTAGGCTAATCA+AGG 0.512881 1_1:+68285738 None:intergenic
GCCAATAATTTGACAGGAGT+AGG 0.513461 1_1:-68284845 Msa0038820:three_prime_UTR
GAGCAACTCTATGTATGCAC+TGG 0.514243 1_1:-68285068 Msa0038820:CDS
GTCTATCAATCTCGAAATTG+TGG 0.522717 1_1:-68285454 Msa0038820:CDS
TTGATTACGCTTCCAACTCA+AGG 0.533033 1_1:-68284995 Msa0038820:CDS
AGGTGCTGCGGCAAGAGTCA+AGG 0.534822 1_1:-68287334 Msa0038820:CDS
TTGATGTTCCTTTGGCAGGA+AGG 0.538933 1_1:+68287269 None:intergenic
GAGTCAAGGAGCTGCTTGAA+GGG 0.541631 1_1:-68287320 Msa0038820:CDS
AAAAGTCCTAAAAGATGTGA+AGG 0.543573 1_1:-68287376 Msa0038820:CDS
GTCACTACCAAGGCCAACAT+AGG 0.547612 1_1:-68285352 Msa0038820:CDS
TCACTACCAAGGCCAACATA+GGG 0.550648 1_1:-68285351 Msa0038820:CDS
CGAGGCAGTACAAGCTAATG+AGG 0.552124 1_1:-68287403 Msa0038820:CDS
GCACATCCAAAGGAAGTTCA+CGG 0.562215 1_1:+68285920 None:intergenic
GCCTCGTCATGGTAGTTCTG+GGG 0.562928 1_1:+68287420 None:intergenic
TTGCAACACTATGTTACTTG+TGG 0.567630 1_1:-68284898 Msa0038820:three_prime_UTR
CAAATCAAACTTGAGACCAC+TGG 0.570634 1_1:-68287249 Msa0038820:intron
CAAGCTGAGTACCCCTATGT+TGG 0.578511 1_1:+68285339 None:intergenic
GCATTTGTCAAAAGCAAGCA+AGG 0.584204 1_1:+68285528 None:intergenic
TTTATGTAGTGTTTATTCAG+GGG 0.586406 1_1:-68285038 Msa0038820:CDS
CATTGAGCAACCCAAATAGG+TGG 0.598653 1_1:-68285099 Msa0038820:CDS
AAAACACCAATCCAACATGA+AGG 0.599108 1_1:+68284964 None:intergenic
ATCACAATTAACTAACCTAA+GGG 0.600746 1_1:+68285261 None:intergenic
ATTCGTCAATAGGAAATGAA+CGG 0.603412 1_1:-68285420 Msa0038820:CDS
GGAGTAGGAAAAGCTTGCAA+TGG 0.606769 1_1:-68284830 Msa0038820:three_prime_UTR
CCTCGTCATGGTAGTTCTGG+GGG 0.607116 1_1:+68287421 None:intergenic
ATGATATCAAGCACATCCAA+AGG 0.608076 1_1:+68285910 None:intergenic
ATTCAATACCTTCCTGCCAA+AGG 0.612236 1_1:-68287277 Msa0038820:CDS
ATTGAGCAACCCAAATAGGT+GGG 0.612873 1_1:-68285098 Msa0038820:CDS
GATCAAAGAATTTATACAAG+AGG 0.613874 1_1:-68287354 Msa0038820:CDS
TAGCTTGTACTGCCTCGTCA+TGG 0.617598 1_1:+68287409 None:intergenic
CGGTGTGTGCAAGAATTGGA+AGG 0.623153 1_1:-68285848 Msa0038820:CDS
CTTCCAATTCTTGCACACAC+CGG 0.626272 1_1:+68285849 None:intergenic
AATTTATACAAGAGGTGCTG+CGG 0.636068 1_1:-68287346 Msa0038820:CDS
AGCAACTCTATGTATGCACT+GGG 0.636113 1_1:-68285067 Msa0038820:CDS
ATCTATGCTACATCTTCTAG+CGG 0.636928 1_1:-68285670 Msa0038820:CDS
AATATATGTTGTCACTACCA+AGG 0.650134 1_1:-68285362 Msa0038820:CDS
CACTACCAAGGCCAACATAG+GGG 0.661007 1_1:-68285350 Msa0038820:CDS
TTGAGCAACCCAAATAGGTG+GGG 0.691982 1_1:-68285097 Msa0038820:CDS
ATTACCATAATGATAAGACT+CGG 0.708713 1_1:+68285555 None:intergenic
GATAAGACTCGGCTTCAAAG+GGG 0.717149 1_1:+68285566 None:intergenic
TCGTCAATAGGAAATGAACG+GGG 0.727167 1_1:-68285418 Msa0038820:CDS
CGTCAATAGGAAATGAACGG+GGG 0.729159 1_1:-68285417 Msa0038820:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTTCAGAAATAAATAAAA+AGG - chr1_1:68286071-68286090 Msa0038820:intron 10.0%
!!! TATATATAGATTACTTTTTC+CGG + chr1_1:68285314-68285333 None:intergenic 15.0%
!!! TGAAAAACTATATTTTAATG+TGG - chr1_1:68285385-68285404 Msa0038820:CDS 15.0%
!!! TTATCATTTTTCTTTTTGTT+GGG - chr1_1:68285785-68285804 Msa0038820:CDS 15.0%
!!! AACTTAAAGCATTAAATATT+AGG + chr1_1:68285836-68285855 None:intergenic 15.0%
!! TATGAAATTTGAAGAATTAA+TGG + chr1_1:68286310-68286329 None:intergenic 15.0%
!!! ATTAATAGTTTGTTTTCTTT+TGG - chr1_1:68287419-68287438 Msa0038820:CDS 15.0%
!! TCAAAATAGTCTCTAAATTA+GGG + chr1_1:68284948-68284967 None:intergenic 20.0%
!! ATCAAAATAGTCTCTAAATT+AGG + chr1_1:68284949-68284968 None:intergenic 20.0%
!!! AGTTTTGCACAATTAATTTA+TGG - chr1_1:68284971-68284990 Msa0038820:CDS 20.0%
!!! TTTTTCATGTAATCAATTGA+TGG - chr1_1:68285028-68285047 Msa0038820:CDS 20.0%
!!! TTTTCATGTAATCAATTGAT+GGG - chr1_1:68285029-68285048 Msa0038820:CDS 20.0%
!! CAACAAAAAGAAAAATGATA+AGG + chr1_1:68285786-68285805 None:intergenic 20.0%
!!! CTTATCATTTTTCTTTTTGT+TGG - chr1_1:68285784-68285803 Msa0038820:CDS 20.0%
!!! TATCATTTTTCTTTTTGTTG+GGG - chr1_1:68285786-68285805 Msa0038820:CDS 20.0%
!! TTCACATGAAGAAAAAAAAA+AGG - chr1_1:68285933-68285952 Msa0038820:CDS 20.0%
!! TGATAATATCATAGAAAATG+AGG + chr1_1:68286033-68286052 None:intergenic 20.0%
!!! TCAAGCAATAATAAACTTTT+TGG + chr1_1:68286372-68286391 None:intergenic 20.0%
!!! CAATTTTAGACAAAGTATTA+AGG - chr1_1:68287291-68287310 Msa0038820:CDS 20.0%
!!! GAAAGATTTTTTGTTTGTTT+TGG - chr1_1:68287319-68287338 Msa0038820:CDS 20.0%
! GATCAAAGAATTTATACAAG+AGG - chr1_1:68284734-68284753 Msa0038820:three_prime_UTR 25.0%
! AATGATAAATTACTAACCAG+TGG + chr1_1:68284858-68284877 None:intergenic 25.0%
! AACATAAGTCAAGATAACAA+GGG + chr1_1:68285212-68285231 None:intergenic 25.0%
! AAACATAAGTCAAGATAACA+AGG + chr1_1:68285213-68285232 None:intergenic 25.0%
!! AAAGCATTAAATATTAGGCA+TGG + chr1_1:68285831-68285850 None:intergenic 25.0%
!!! TGTTTGCTATACATTGAAAA+TGG - chr1_1:68285898-68285917 Msa0038820:CDS 25.0%
!!! GTTTGCTATACATTGAAAAT+GGG - chr1_1:68285899-68285918 Msa0038820:CDS 25.0%
! TATACATTGAAAATGGGAAA+AGG - chr1_1:68285905-68285924 Msa0038820:CDS 25.0%
! ATCACAACAATGACAAAATT+AGG + chr1_1:68286114-68286133 None:intergenic 25.0%
! AATTTACTGGAGAAATTTCT+TGG - chr1_1:68286273-68286292 Msa0038820:intron 25.0%
! TTGAAGAATTAATGGTTCTT+GGG + chr1_1:68286302-68286321 None:intergenic 25.0%
! TTTGAAGAATTAATGGTTCT+TGG + chr1_1:68286303-68286322 None:intergenic 25.0%
!!! CAATAATAAACTTTTTGGTC+GGG + chr1_1:68286367-68286386 None:intergenic 25.0%
!!! GCAATAATAAACTTTTTGGT+CGG + chr1_1:68286368-68286387 None:intergenic 25.0%
! TCCATTTACAAGAATAAAGA+AGG - chr1_1:68286486-68286505 Msa0038820:intron 25.0%
! ACCTTCTTTATTCTTGTAAA+TGG + chr1_1:68286490-68286509 None:intergenic 25.0%
! ATTACCATAATGATAAGACT+CGG + chr1_1:68286536-68286555 None:intergenic 25.0%
! ATCACAATTAACTAACCTAA+GGG + chr1_1:68286830-68286849 None:intergenic 25.0%
!!! AACTTTTAGTGGTTGATTTA+AGG - chr1_1:68286854-68286873 Msa0038820:intron 25.0%
!!! CATTGCATTATTTTATGCTT+TGG - chr1_1:68286951-68286970 Msa0038820:intron 25.0%
! CTTTATGTAGTGTTTATTCA+GGG - chr1_1:68287049-68287068 Msa0038820:intron 25.0%
! TTTATGTAGTGTTTATTCAG+GGG - chr1_1:68287050-68287069 Msa0038820:intron 25.0%
!! TTTTAGACATCTACAATATG+AGG - chr1_1:68287143-68287162 Msa0038820:intron 25.0%
! CAAATTATTGGCAAACAAAA+AGG + chr1_1:68287235-68287254 None:intergenic 25.0%
!!! TTTTGTTTGTTTTGGAAACA+AGG - chr1_1:68287327-68287346 Msa0038820:CDS 25.0%
! TGTGTATGAATTGAATATGT+TGG - chr1_1:68287394-68287413 Msa0038820:CDS 25.0%
AAAAGTCCTAAAAGATGTGA+AGG - chr1_1:68284712-68284731 Msa0038820:three_prime_UTR 30.0%
! TTTGATCCTTCACATCTTTT+AGG + chr1_1:68284721-68284740 None:intergenic 30.0%
!! AGTTTGATTTGATGTTCCTT+TGG + chr1_1:68284830-68284849 None:intergenic 30.0%
!! ATGTAATCAATTGATGGGTT+TGG - chr1_1:68285034-68285053 Msa0038820:CDS 30.0%
ATTAAGCTCAATGAAATCCT+AGG + chr1_1:68285591-68285610 None:intergenic 30.0%
GAAAAAAAAATCAAGCACAG+CGG + chr1_1:68285630-68285649 None:intergenic 30.0%
!!! TTTTTTTCTCAGACGATTGT+AGG - chr1_1:68285704-68285723 Msa0038820:CDS 30.0%
!!! TTTTTTCTCAGACGATTGTA+GGG - chr1_1:68285705-68285724 Msa0038820:CDS 30.0%
ATGATAAGGATTAAAGACGT+AGG + chr1_1:68285772-68285791 None:intergenic 30.0%
!!! TTAGAGTGTTATTTTCCATG+AGG - chr1_1:68285854-68285873 Msa0038820:CDS 30.0%
! GATATCTATTTTCATCCTCA+TGG + chr1_1:68285872-68285891 None:intergenic 30.0%
AGATGTTTGTTTGGCAAAAA+TGG - chr1_1:68285986-68286005 Msa0038820:intron 30.0%
TTGTCATTGTTGTGATCTAA+AGG - chr1_1:68286118-68286137 Msa0038820:intron 30.0%
TGTCATTGTTGTGATCTAAA+GGG - chr1_1:68286119-68286138 Msa0038820:intron 30.0%
!! TTCTAGCGGATATTTTATCA+TGG - chr1_1:68286432-68286451 Msa0038820:intron 30.0%
TGGATTACTTATTCGTCAAT+AGG - chr1_1:68286658-68286677 Msa0038820:intron 30.0%
ATTCGTCAATAGGAAATGAA+CGG - chr1_1:68286668-68286687 Msa0038820:intron 30.0%
AATATATGTTGTCACTACCA+AGG - chr1_1:68286726-68286745 Msa0038820:intron 30.0%
CTAAGGGAAAATGTTTCATT+GGG + chr1_1:68286814-68286833 None:intergenic 30.0%
CATCACAATTAACTAACCTA+AGG + chr1_1:68286831-68286850 None:intergenic 30.0%
AACCATGAATTATGTCAAAC+TGG - chr1_1:68286915-68286934 Msa0038820:intron 30.0%
CCTGAATAAACACTACATAA+AGG + chr1_1:68287051-68287070 None:intergenic 30.0%
CCTTTATGTAGTGTTTATTC+AGG - chr1_1:68287048-68287067 Msa0038820:intron 30.0%
TTGGCAAACAAAAAGGAAAT+AGG + chr1_1:68287228-68287247 None:intergenic 30.0%
TTGTTTGCCAATAATTTGAC+AGG - chr1_1:68287237-68287256 Msa0038820:intron 30.0%
!! AATTTATACAAGAGGTGCTG+CGG - chr1_1:68284742-68284761 Msa0038820:three_prime_UTR 35.0%
AGTCTCTAAATTAGGGCATA+TGG + chr1_1:68284941-68284960 None:intergenic 35.0%
! TGGGTTTGGAGTATTATAAC+AGG - chr1_1:68285048-68285067 Msa0038820:CDS 35.0%
! GGGTTTGGAGTATTATAACA+GGG - chr1_1:68285049-68285068 Msa0038820:CDS 35.0%
! GGTTTGGAGTATTATAACAG+GGG - chr1_1:68285050-68285069 Msa0038820:CDS 35.0%
GAGTATTATAACAGGGGTTA+GGG - chr1_1:68285056-68285075 Msa0038820:CDS 35.0%
AGTATTATAACAGGGGTTAG+GGG - chr1_1:68285057-68285076 Msa0038820:CDS 35.0%
! TAGGGGTTCATGCAATTTTA+TGG - chr1_1:68285074-68285093 Msa0038820:CDS 35.0%
AAATAGAGTCCTAGATAGTG+TGG + chr1_1:68285247-68285266 None:intergenic 35.0%
! ATTTTGTCGCTCTATTTAGC+CGG - chr1_1:68285292-68285311 Msa0038820:CDS 35.0%
ACTACTACAGTTGCAATCAA+CGG + chr1_1:68285358-68285377 None:intergenic 35.0%
CTGAAGAACACACTAATTGT+TGG + chr1_1:68285522-68285541 None:intergenic 35.0%
GTGTTCTTCAGTAACAAAAG+TGG - chr1_1:68285531-68285550 Msa0038820:CDS 35.0%
TCAGTAACAAAAGTGGAAAG+TGG - chr1_1:68285538-68285557 Msa0038820:CDS 35.0%
AACAAAAGTGGAAAGTGGAA+AGG - chr1_1:68285543-68285562 Msa0038820:CDS 35.0%
! CTATTAGTTCTTCACTACCT+AGG - chr1_1:68285571-68285590 Msa0038820:CDS 35.0%
! TTTTTCTCAGACGATTGTAG+GGG - chr1_1:68285706-68285725 Msa0038820:CDS 35.0%
TAGGAAACTGTCAATTAGTG+AGG + chr1_1:68285753-68285772 None:intergenic 35.0%
! TTTTCTTTTTGTTGGGGAGT+AGG - chr1_1:68285792-68285811 Msa0038820:CDS 35.0%
! TTTCTTTTTGTTGGGGAGTA+GGG - chr1_1:68285793-68285812 Msa0038820:CDS 35.0%
! TGCTGTTCTAGATGTTTGTT+TGG - chr1_1:68285977-68285996 Msa0038820:intron 35.0%
GTTGTGATCTAAAGGGAAAA+AGG - chr1_1:68286126-68286145 Msa0038820:intron 35.0%
TAAAGGGAAAAAGGTGAAGA+AGG - chr1_1:68286135-68286154 Msa0038820:intron 35.0%
ATGATATCAAGCACATCCAA+AGG + chr1_1:68286181-68286200 None:intergenic 35.0%
GTCGACAAAATCTAGTGTTT+GGG + chr1_1:68286206-68286225 None:intergenic 35.0%
! AGGCTTTTCAGACAATTTAC+TGG - chr1_1:68286260-68286279 Msa0038820:intron 35.0%
!! AATAAACTTTTTGGTCGGGT+AGG + chr1_1:68286363-68286382 None:intergenic 35.0%
ATCTATGCTACATCTTCTAG+CGG - chr1_1:68286418-68286437 Msa0038820:intron 35.0%
!! TAGCGGATATTTTATCATGG+TGG - chr1_1:68286435-68286454 Msa0038820:intron 35.0%
! AGCGGATATTTTATCATGGT+GGG - chr1_1:68286436-68286455 Msa0038820:intron 35.0%
!! ATGCTCTGAGGAATTTATCT+TGG - chr1_1:68286579-68286598 Msa0038820:intron 35.0%
!!! TGGTTTTTTGCTGCTATCTT+TGG - chr1_1:68286602-68286621 Msa0038820:intron 35.0%
GTCTATCAATCTCGAAATTG+TGG - chr1_1:68286634-68286653 Msa0038820:intron 35.0%
ATCAATCTCGAAATTGTGGT+TGG - chr1_1:68286638-68286657 Msa0038820:intron 35.0%
TTCGTCAATAGGAAATGAAC+GGG - chr1_1:68286669-68286688 Msa0038820:intron 35.0%
CCTAAGGGAAAATGTTTCAT+TGG + chr1_1:68286815-68286834 None:intergenic 35.0%
! CCAATGAAACATTTTCCCTT+AGG - chr1_1:68286812-68286831 Msa0038820:intron 35.0%
! TTGTGATGAGCAACTTTTAG+TGG - chr1_1:68286843-68286862 Msa0038820:intron 35.0%
! ATTTAAGGTTCGACGAAGTA+AGG - chr1_1:68286869-68286888 Msa0038820:intron 35.0%
CTCCAGTTTGACATAATTCA+TGG + chr1_1:68286920-68286939 None:intergenic 35.0%
TTATGTCAAACTGGAGACTT+TGG - chr1_1:68286924-68286943 Msa0038820:intron 35.0%
TATGTCAAACTGGAGACTTT+GGG - chr1_1:68286925-68286944 Msa0038820:intron 35.0%
AAAACACCAATCCAACATGA+AGG + chr1_1:68287127-68287146 None:intergenic 35.0%
! TTGCAACACTATGTTACTTG+TGG - chr1_1:68287190-68287209 Msa0038820:intron 35.0%
TCCTACTCCTGTCAAATTAT+TGG + chr1_1:68287247-68287266 None:intergenic 35.0%
ATTCAATACCTTCCTGCCAA+AGG - chr1_1:68284811-68284830 Msa0038820:three_prime_UTR 40.0%
TGATTTGATGTTCCTTTGGC+AGG + chr1_1:68284826-68284845 None:intergenic 40.0%
CAAATCAAACTTGAGACCAC+TGG - chr1_1:68284839-68284858 Msa0038820:three_prime_UTR 40.0%
! ATGGATTAAGTTGCGACACT+CGG - chr1_1:68284990-68285009 Msa0038820:CDS 40.0%
! GGAGTATTATAACAGGGGTT+AGG - chr1_1:68285055-68285074 Msa0038820:CDS 40.0%
TAATTCTTCCCGAGCATCTA+TGG - chr1_1:68285148-68285167 Msa0038820:CDS 40.0%
AATTCTTCCCGAGCATCTAT+GGG - chr1_1:68285149-68285168 Msa0038820:CDS 40.0%
ATTACATCCCCATAGATGCT+CGG + chr1_1:68285160-68285179 None:intergenic 40.0%
AGCATCTATGGGGATGTAAT+TGG - chr1_1:68285160-68285179 Msa0038820:CDS 40.0%
AAGTCAAGATAACAAGGGCT+GGG + chr1_1:68285207-68285226 None:intergenic 40.0%
TAAGTCAAGATAACAAGGGC+TGG + chr1_1:68285208-68285227 None:intergenic 40.0%
GTTTACTGTCCACACTATCT+AGG - chr1_1:68285235-68285254 Msa0038820:CDS 40.0%
AACGTCATGACTGTTTGTGA+TGG - chr1_1:68285427-68285446 Msa0038820:CDS 40.0%
! TTGTGATGGAAAAGAACACG+AGG - chr1_1:68285441-68285460 Msa0038820:CDS 40.0%
! TTCTTTTTGTTGGGGAGTAG+GGG - chr1_1:68285794-68285813 Msa0038820:CDS 40.0%
GGTCGACAAAATCTAGTGTT+TGG + chr1_1:68286207-68286226 None:intergenic 40.0%
!!! GTCGACCTTTTTCAATTTGC+CGG - chr1_1:68286220-68286239 Msa0038820:intron 40.0%
ACACACCGGCAAATTGAAAA+AGG + chr1_1:68286228-68286247 None:intergenic 40.0%
! GCGGATATTTTATCATGGTG+GGG - chr1_1:68286437-68286456 Msa0038820:intron 40.0%
TGATAAGACTCGGCTTCAAA+GGG + chr1_1:68286526-68286545 None:intergenic 40.0%
ATGATAAGACTCGGCTTCAA+AGG + chr1_1:68286527-68286546 None:intergenic 40.0%
! GAAGCCGAGTCTTATCATTA+TGG - chr1_1:68286529-68286548 Msa0038820:intron 40.0%
GCATTTGTCAAAAGCAAGCA+AGG + chr1_1:68286563-68286582 None:intergenic 40.0%
! TGCTTTTGACAAATGCTCTG+AGG - chr1_1:68286567-68286586 Msa0038820:intron 40.0%
! CTCTGAGGAATTTATCTTGG+TGG - chr1_1:68286582-68286601 Msa0038820:intron 40.0%
TCGTCAATAGGAAATGAACG+GGG - chr1_1:68286670-68286689 Msa0038820:intron 40.0%
ATGCATTGAGCAACCCAAAT+AGG - chr1_1:68286986-68287005 Msa0038820:intron 40.0%
ATTGAGCAACCCAAATAGGT+GGG - chr1_1:68286990-68287009 Msa0038820:intron 40.0%
TCTCATATCCCCACCTATTT+GGG + chr1_1:68287002-68287021 None:intergenic 40.0%
AGCAACTCTATGTATGCACT+GGG - chr1_1:68287021-68287040 Msa0038820:intron 40.0%
TTGATTACGCTTCCAACTCA+AGG - chr1_1:68287093-68287112 Msa0038820:intron 40.0%
CAAGTTCCTTCATGTTGGAT+TGG - chr1_1:68287118-68287137 Msa0038820:intron 40.0%
GCCAATAATTTGACAGGAGT+AGG - chr1_1:68287243-68287262 Msa0038820:intron 40.0%
TTGATGTTCCTTTGGCAGGA+AGG + chr1_1:68284822-68284841 None:intergenic 45.0%
ATTCTTCCCGAGCATCTATG+GGG - chr1_1:68285150-68285169 Msa0038820:CDS 45.0%
TTACATCCCCATAGATGCTC+GGG + chr1_1:68285159-68285178 None:intergenic 45.0%
AGTCAAGATAACAAGGGCTG+GGG + chr1_1:68285206-68285225 None:intergenic 45.0%
GGAAAACTTTCTGACTGCAG+AGG + chr1_1:68285337-68285356 None:intergenic 45.0%
GACGCTTTGACACTCTCTTA+TGG + chr1_1:68285664-68285683 None:intergenic 45.0%
ACGATTGTAGGGGAATTGCA+TGG - chr1_1:68285716-68285735 Msa0038820:CDS 45.0%
GTAGGGGAATTGCATGGAAA+TGG - chr1_1:68285722-68285741 Msa0038820:CDS 45.0%
AGAGAACCGTGAACTTCCTT+TGG - chr1_1:68286162-68286181 Msa0038820:intron 45.0%
GCACATCCAAAGGAAGTTCA+CGG + chr1_1:68286171-68286190 None:intergenic 45.0%
CTTCCAATTCTTGCACACAC+CGG + chr1_1:68286242-68286261 None:intergenic 45.0%
GATAAGACTCGGCTTCAAAG+GGG + chr1_1:68286525-68286544 None:intergenic 45.0%
CGTCAATAGGAAATGAACGG+GGG - chr1_1:68286671-68286690 Msa0038820:intron 45.0%
AATAGGAAATGAACGGGGGA+GGG - chr1_1:68286675-68286694 Msa0038820:intron 45.0%
TCACTACCAAGGCCAACATA+GGG - chr1_1:68286737-68286756 Msa0038820:intron 45.0%
CATTGAGCAACCCAAATAGG+TGG - chr1_1:68286989-68287008 Msa0038820:intron 45.0%
TTGAGCAACCCAAATAGGTG+GGG - chr1_1:68286991-68287010 Msa0038820:intron 45.0%
CTCTCATATCCCCACCTATT+TGG + chr1_1:68287003-68287022 None:intergenic 45.0%
GAGCAACTCTATGTATGCAC+TGG - chr1_1:68287020-68287039 Msa0038820:intron 45.0%
! AAGGAACTTGCTCCTTGAGT+TGG + chr1_1:68287108-68287127 None:intergenic 45.0%
AGGAGCAAGTTCCTTCATGT+TGG - chr1_1:68287113-68287132 Msa0038820:intron 45.0%
GGAGTAGGAAAAGCTTGCAA+TGG - chr1_1:68287258-68287277 Msa0038820:CDS 45.0%
TGCCTCGTCATGGTAGTTCT+GGG + chr1_1:68284672-68284691 None:intergenic 50.0%
! TAGCTTGTACTGCCTCGTCA+TGG + chr1_1:68284682-68284701 None:intergenic 50.0%
CGAGGCAGTACAAGCTAATG+AGG - chr1_1:68284685-68284704 Msa0038820:three_prime_UTR 50.0%
AGAGTCAAGGAGCTGCTTGA+AGG - chr1_1:68284767-68284786 Msa0038820:three_prime_UTR 50.0%
GAGTCAAGGAGCTGCTTGAA+GGG - chr1_1:68284768-68284787 Msa0038820:three_prime_UTR 50.0%
! TGTTTTACGCGAGCGAGCAT+TGG - chr1_1:68285112-68285131 Msa0038820:CDS 50.0%
ATAACAAGGGCTGGGGAATG+GGG + chr1_1:68285199-68285218 None:intergenic 50.0%
GATAACAAGGGCTGGGGAAT+GGG + chr1_1:68285200-68285219 None:intergenic 50.0%
AGATAACAAGGGCTGGGGAA+TGG + chr1_1:68285201-68285220 None:intergenic 50.0%
TTGCCGGTGTGTGCAAGAAT+TGG - chr1_1:68286236-68286255 Msa0038820:intron 50.0%
CGGTGTGTGCAAGAATTGGA+AGG - chr1_1:68286240-68286259 Msa0038820:intron 50.0%
TTGGTCGGGTAGGCTAATCA+AGG + chr1_1:68286353-68286372 None:intergenic 50.0%
CAATAGGAAATGAACGGGGG+AGG - chr1_1:68286674-68286693 Msa0038820:intron 50.0%
GTCACTACCAAGGCCAACAT+AGG - chr1_1:68286736-68286755 Msa0038820:intron 50.0%
CACTACCAAGGCCAACATAG+GGG - chr1_1:68286738-68286757 Msa0038820:intron 50.0%
CAAGCTGAGTACCCCTATGT+TGG + chr1_1:68286752-68286771 None:intergenic 50.0%
!! CCTCGTCATGGTAGTTCTGG+GGG + chr1_1:68284670-68284689 None:intergenic 55.0%
! GCCTCGTCATGGTAGTTCTG+GGG + chr1_1:68284671-68284690 None:intergenic 55.0%
CTGCCTCGTCATGGTAGTTC+TGG + chr1_1:68284673-68284692 None:intergenic 55.0%
!! TACGCGAGCGAGCATTGGAA+AGG - chr1_1:68285117-68285136 Msa0038820:CDS 55.0%
!! ACGCGAGCGAGCATTGGAAA+GGG - chr1_1:68285118-68285137 Msa0038820:CDS 55.0%
! GAGTACCCCTATGTTGGCCT+TGG + chr1_1:68286746-68286765 None:intergenic 55.0%
CCCCCAGAACTACCATGACG+AGG - chr1_1:68284667-68284686 Msa0038820:three_prime_UTR 60.0%
! AGGTGCTGCGGCAAGAGTCA+AGG - chr1_1:68284754-68284773 Msa0038820:three_prime_UTR 60.0%
!! CGAGCGAGCATTGGAAAGGG+TGG - chr1_1:68285121-68285140 Msa0038820:CDS 60.0%
AGGAAATGAACGGGGGAGGG+TGG - chr1_1:68286678-68286697 Msa0038820:intron 60.0%
! GGGGAGGGTGGTTGACTTTG+TGG - chr1_1:68286690-68286709 Msa0038820:intron 60.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 68284659 68287451 68284659 ID=Msa0038820;Name=Msa0038820
chr1_1 mRNA 68284659 68287451 68284659 ID=Msa0038820-mRNA-1;Parent=Msa0038820;Name=Msa0038820-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0|1|0.5|1|1|1|2|263|416
chr1_1 exon 68284659 68285970 68284659 ID=Msa0038820-mRNA-1:exon:22201;Parent=Msa0038820-mRNA-1
chr1_1 exon 68287250 68287451 68287250 ID=Msa0038820-mRNA-1:exon:22200;Parent=Msa0038820-mRNA-1
chr1_1 CDS 68287250 68287451 68287250 ID=Msa0038820-mRNA-1:cds;Parent=Msa0038820-mRNA-1
chr1_1 CDS 68284922 68285970 68284922 ID=Msa0038820-mRNA-1:cds;Parent=Msa0038820-mRNA-1
chr1_1 three_prime_UTR 68284659 68284921 68284659 ID=Msa0038820-mRNA-1:three_prime_utr;Parent=Msa0038820-mRNA-1
Gene Sequence

>Msa0038820

ATGAAGATCCCCCAGAACTACCATGACGAGGCAGTACAAGCTAATGAGGTTTTAAAAGTCCTAAAAGATGTGAAGGATCAAAGAATTTATACAAGAGGTGCTGCGGCAAGAGTCAAGGAGCTGCTTGAAGGGCATACTCATTTAATTTTGAGATTCAATACCTTCCTGCCAAAGGAACATCAAATCAAACTTGAGACCACTGGGAAAAAGGTGAAGAAGGTAGAAGAGAACCGTGAACTTCCTTTGGATGTGCTTGATATCATTTCCCAAACACTAGATTTTGTCGACCTTTTTCAATTTGCCGGTGTGTGCAAGAATTGGAAGGCTTTTCAGACAATTTACTGGAGAAATTTCTTGGCATCCCAAGAACCATTAATTCTTCAAATTTCATATTTTTGTACTAGATCAATTTCCTTGATTAGCCTACCCGACCAAAAAGTTTATTATTGCTTGAAGATAAAGAATTACTTCAACGAATATATCTATGCTACATCTTCTAGCGGATATTTTATCATGGTGGGGAGAAATAATTCAGTTTTGCTAATCAATCCATTTACAAGAATAAAGAAGGTAATCAATAACTGCCCCTTTGAAGCCGAGTCTTATCATTATGGTAATCGTGCCTTGCTTGCTTTTGACAAATGCTCTGAGGAATTTATCTTGGTGGTTTTTTGCTGCTATCTTTGGAGTTTACATGTCTATCAATCTCGAAATTGTGGTTGGATTACTTATTCGTCAATAGGAAATGAACGGGGGAGGGTGGTTGACTTTGTGGTCTTGCATAATATAATATATGTTGTCACTACCAAGGCCAACATAGGGGTACTCAGCTTGACTTCTGCAAATATTAAATTTCTAAATTTGAAGAATACTCCCAATGAAACATTTTCCCTTAGGTTAGTTAATTGTGATGAGCAACTTTTAGTGGTTGATTTAAGGTTCGACGAAGTAAGGAATGTGTACAAGATAGATTTCTCAACCATGAATTATGTCAAACTGGAGACTTTGGGCGACATTGCATTATTTTATGCTTTGGACAGAAATTTTTATGCATTGAGCAACCCAAATAGGTGGGGATATGAGAGCAACTCTATGTATGCACTGGGAAATCCTTTATGTAGTGTTTATTCAGGGGATGATAAAAAGTTGCAAAAATTGATTACGCTTCCAACTCAAGGAGCAAGTTCCTTCATGTTGGATTGGTGTTTTAGACATCTACAATATGAGGTAGATTATTCTCTAGTCGAGTGA
Protein sequence

>Msa0038820

MKIPQNYHDEAVQANEVLKVLKDVKDQRIYTRGAAARVKELLEGHTHLILRFNTFLPKEHQIKLETTGKKVKKVEENRELPLDVLDIISQTLDFVDLFQFAGVCKNWKAFQTIYWRNFLASQEPLILQISYFCTRSISLISLPDQKVYYCLKIKNYFNEYIYATSSSGYFIMVGRNNSVLLINPFTRIKKVINNCPFEAESYHYGNRALLAFDKCSEEFILVVFCCYLWSLHVYQSRNCGWITYSSIGNERGRVVDFVVLHNIIYVVTTKANIGVLSLTSANIKFLNLKNTPNETFSLRLVNCDEQLLVVDLRFDEVRNVYKIDFSTMNYVKLETLGDIALFYALDRNFYALSNPNRWGYESNSMYALGNPLCSVYSGDDKKLQKLITLPTQGASSFMLDWCFRHLQYEVDYSLVE*