AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0040800

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0040800 MtrunA17_Chr1g0206091 100.000 96 0 0 1 96 1 96 1.73e-67 197
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0040800 AT1G61780.1 88.776 98 9 1 1 96 1 98 7.77e-60 178

Find 39 sgRNAs with CRISPR-Local

Find 234 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAAGGAACCTGTTCTTCT+TGG 0.284589 1_1:-71070760 None:intergenic
ATACTCGAAGGCTGTCGTTT+TGG 0.306400 1_1:+71070401 Msa0040800:five_prime_UTR
TCAATTTGAATATCAAGATT+TGG 0.309009 1_1:-71070479 None:intergenic
ACCTCCCCTCTCATAAGTTT+TGG 0.331989 1_1:-71073920 None:intergenic
GATCAATTAGACTATATTAA+AGG 0.332949 1_1:-71073701 None:intergenic
AACTTATGAGAGGGGAGGTT+TGG 0.355359 1_1:+71073924 Msa0040800:three_prime_UTR
GCACCTTCTTTCCATTTGTC+TGG 0.356085 1_1:-71070684 None:intergenic
AATTAGCGGAAAAGGGGTAT+AGG 0.363879 1_1:+71070448 Msa0040800:five_prime_UTR
ATTAGCGGAAAAGGGGTATA+GGG 0.384558 1_1:+71070449 Msa0040800:five_prime_UTR
AACTCCTTTCCAAGAAGAAC+AGG 0.405714 1_1:+71070751 Msa0040800:CDS
ACGACAGGAATTAGCGGAAA+AGG 0.420345 1_1:+71070440 Msa0040800:five_prime_UTR
CGACAGGAATTAGCGGAAAA+GGG 0.432678 1_1:+71070441 Msa0040800:five_prime_UTR
CCTTGCAGATGGACCCCTTA+CGG 0.442126 1_1:+71073398 Msa0040800:intron
ATTGCTTTGTTTATAAAACT+TGG 0.455004 1_1:-71073624 None:intergenic
AGGTGATAGTACCAGACAAA+TGG 0.467879 1_1:+71070673 Msa0040800:CDS
CACACGTGTGCTTACTCAAA+AGG 0.468563 1_1:+71073476 Msa0040800:CDS
TGCACTTAGTTGTTCCGTAA+GGG 0.517165 1_1:-71073412 None:intergenic
TTGCAAGCAGCAAGTGCACC+AGG 0.519611 1_1:+71073439 Msa0040800:CDS
GAACAAAAGTTCAAAACGAC+AGG 0.524620 1_1:+71070425 Msa0040800:five_prime_UTR
ATGCACTTAGTTGTTCCGTA+AGG 0.525449 1_1:-71073413 None:intergenic
GGAACCTGTTCTTCTTGGAA+AGG 0.539127 1_1:-71070755 None:intergenic
TGTGACAGTACTTGCCATCC+TGG 0.544753 1_1:-71073457 None:intergenic
GCACTTAGTTGTTCCGTAAG+GGG 0.546613 1_1:-71073411 None:intergenic
TTTGACCAAAACTTATGAGA+GGG 0.549382 1_1:+71073915 Msa0040800:three_prime_UTR
ATAAACAAAGCAATGTGTAG+TGG 0.551489 1_1:+71073633 Msa0040800:CDS
TTCAAAACGACAGGAATTAG+CGG 0.551872 1_1:+71070434 Msa0040800:five_prime_UTR
GTACCAGACAAATGGAAAGA+AGG 0.553949 1_1:+71070681 Msa0040800:CDS
AGGTCAGAAGAAATTATCGA+AGG 0.591221 1_1:+71070653 Msa0040800:intron
TTGACCAAAACTTATGAGAG+GGG 0.600020 1_1:+71073916 Msa0040800:three_prime_UTR
GGTGCAAGCAATACTACTGA+AGG 0.608421 1_1:+71070702 Msa0040800:CDS
GCAGGGGTCTGTGCGATGTG+TGG 0.611108 1_1:+71073584 Msa0040800:intron
ACCAAAACTTATGAGAGGGG+AGG 0.613114 1_1:+71073919 Msa0040800:three_prime_UTR
TTTCTCATTATCGACTGCGA+TGG 0.614991 1_1:+71070535 Msa0040800:exon
AGCAATACTACTGAAGGCGG+TGG 0.615667 1_1:+71070708 Msa0040800:CDS
GACAGGAATTAGCGGAAAAG+GGG 0.616129 1_1:+71070442 Msa0040800:five_prime_UTR
AAGCAGCAAGTGCACCAGGA+TGG 0.627659 1_1:+71073443 Msa0040800:CDS
TAGATCTCACATATACTCGA+AGG 0.633173 1_1:+71070389 Msa0040800:five_prime_UTR
GCAAGCAATACTACTGAAGG+CGG 0.661649 1_1:+71070705 Msa0040800:CDS
GGAGGTTTGGTAGATGAATG+CGG 0.687395 1_1:+71073937 Msa0040800:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTGAAATTAACAATTATTT+TGG - chr1_1:71071700-71071719 None:intergenic 10.0%
!! AAAACAAATAAGAAATTGAA+GGG - chr1_1:71070793-71070812 None:intergenic 15.0%
!! AAAAACAAATAAGAAATTGA+AGG - chr1_1:71070794-71070813 None:intergenic 15.0%
!!! ATATTCTTTGATTTCTTTTT+TGG + chr1_1:71072597-71072616 Msa0040800:intron 15.0%
!! AAAAGAAAACTTAAAAGTTT+GGG - chr1_1:71072920-71072939 None:intergenic 15.0%
!! AAAAAGAAAACTTAAAAGTT+TGG - chr1_1:71072921-71072940 None:intergenic 15.0%
!! TAAACAATTACAAAAAGTAA+TGG - chr1_1:71073242-71073261 None:intergenic 15.0%
!! AACAAGTTACAATATAAAAA+AGG - chr1_1:71073320-71073339 None:intergenic 15.0%
!! TTCAAAAAGTAAATTACATA+TGG - chr1_1:71073792-71073811 None:intergenic 15.0%
!! TCAATTTGAATATCAAGATT+TGG - chr1_1:71070482-71070501 None:intergenic 20.0%
!! ACTAGAAATATTGAAGAAAA+AGG - chr1_1:71070586-71070605 None:intergenic 20.0%
!!! GAAAATTCAATTGATCTTTT+TGG + chr1_1:71070610-71070629 Msa0040800:intron 20.0%
!! AACTAAGAAACAAATTATGA+AGG - chr1_1:71070823-71070842 None:intergenic 20.0%
!!! TTGTTTCTTAGTTTTTAAGT+AGG + chr1_1:71070830-71070849 Msa0040800:intron 20.0%
!! CAAAGTCTATTTATTTGTTT+AGG - chr1_1:71071071-71071090 None:intergenic 20.0%
!!! TTTAAACTTACTCTTTTGTA+GGG + chr1_1:71071208-71071227 Msa0040800:intron 20.0%
!! TAATTGTTAATTTCAATAGC+TGG + chr1_1:71071703-71071722 Msa0040800:intron 20.0%
!! AATTGTTAATTTCAATAGCT+GGG + chr1_1:71071704-71071723 Msa0040800:intron 20.0%
!! ACTAGAAAATTACAGAATAT+GGG - chr1_1:71071744-71071763 None:intergenic 20.0%
!! AACTAGAAAATTACAGAATA+TGG - chr1_1:71071745-71071764 None:intergenic 20.0%
!! AAATATTTGACATGTGAAAT+TGG - chr1_1:71071909-71071928 None:intergenic 20.0%
!! TCTATCATTTACTTTGAATT+CGG - chr1_1:71071970-71071989 None:intergenic 20.0%
!!! AATGTTATTTTTCTTTCAGA+TGG - chr1_1:71072223-71072242 None:intergenic 20.0%
!! AATGACTATTAGTATTAGTA+TGG + chr1_1:71073114-71073133 Msa0040800:intron 20.0%
!!! ATTGCTTTGTTTATAAAACT+TGG - chr1_1:71073627-71073646 None:intergenic 20.0%
!! GATCAATTAGACTATATTAA+AGG - chr1_1:71073704-71073723 None:intergenic 20.0%
!! CTGTAAAAAGTTAATATTGT+GGG + chr1_1:71073748-71073767 Msa0040800:three_prime_UTR 20.0%
! AATAAGAAATTGAAGGGTTT+AGG - chr1_1:71070787-71070806 None:intergenic 25.0%
!! TACTTCAAATTTGAGATACT+TGG + chr1_1:71070964-71070983 Msa0040800:intron 25.0%
!! CTTTAAACTTACTCTTTTGT+AGG + chr1_1:71071207-71071226 Msa0040800:intron 25.0%
! TATTGACAATGGAATTGATA+AGG + chr1_1:71071239-71071258 Msa0040800:intron 25.0%
! ATGGAAAAACTAAGTTCAAA+TGG + chr1_1:71071362-71071381 Msa0040800:intron 25.0%
! ACTAAGTTCAAATGGAAAAT+TGG + chr1_1:71071370-71071389 Msa0040800:intron 25.0%
! AATTGGAAATAGATAACACT+AGG + chr1_1:71071387-71071406 Msa0040800:intron 25.0%
!!! CAATTATTTTGGTACTTTGA+TGG - chr1_1:71071689-71071708 None:intergenic 25.0%
! CTAGAAAATTACAGAATATG+GGG - chr1_1:71071743-71071762 None:intergenic 25.0%
!!! TCTGTAATTTTCTAGTTTAG+TGG + chr1_1:71071748-71071767 Msa0040800:intron 25.0%
! ACAAATTCATACAAAGTTTG+AGG + chr1_1:71071819-71071838 Msa0040800:intron 25.0%
!!! CAAATATTTTTGAGAAAGCA+TGG + chr1_1:71071920-71071939 Msa0040800:intron 25.0%
! CTATCATTTACTTTGAATTC+GGG - chr1_1:71071969-71071988 None:intergenic 25.0%
!! TAATTTTGGATTTCAGTTCA+TGG - chr1_1:71072038-71072057 None:intergenic 25.0%
!! GGTAAATGGTTTTCTAATTT+TGG - chr1_1:71072052-71072071 None:intergenic 25.0%
! ATTAGAAAACCATTTACCAA+TGG + chr1_1:71072054-71072073 Msa0040800:intron 25.0%
! ATAGATACAATGAATTCACT+AGG + chr1_1:71072282-71072301 Msa0040800:intron 25.0%
!!! AGCTTTATTCCTTGAATTAT+TGG + chr1_1:71072490-71072509 Msa0040800:intron 25.0%
! TCAAAGTAAAGTCAACAAAT+TGG - chr1_1:71072545-71072564 None:intergenic 25.0%
! TTGACTTTACTTTGATGAAT+TGG + chr1_1:71072550-71072569 Msa0040800:intron 25.0%
! TTTCAATAGTAGGTTTGATA+TGG + chr1_1:71072630-71072649 Msa0040800:intron 25.0%
!! GAAAGAAACCTAATGTTTTT+AGG + chr1_1:71072673-71072692 Msa0040800:intron 25.0%
! AAAAAAAACTGAATTGTCGA+GGG - chr1_1:71072890-71072909 None:intergenic 25.0%
! AAAAAAAAACTGAATTGTCG+AGG - chr1_1:71072891-71072910 None:intergenic 25.0%
!! ATATCTTTGGTGTATATATG+TGG + chr1_1:71073002-71073021 Msa0040800:intron 25.0%
! GTCATTTAAACAAAAGATCA+AGG - chr1_1:71073287-71073306 None:intergenic 25.0%
! TTTAATTAATTCCTTGCAGA+TGG + chr1_1:71073387-71073406 Msa0040800:intron 25.0%
! GCTGTAAAAAGTTAATATTG+TGG + chr1_1:71073747-71073766 Msa0040800:three_prime_UTR 25.0%
!! TTTTTGGTGATTTGAATTCG+TGG + chr1_1:71070626-71070645 Msa0040800:intron 30.0%
! TGATTTGAATTCGTGGTTTT+AGG + chr1_1:71070633-71070652 Msa0040800:intron 30.0%
TCGTAGTTGTATTGTTTCAT+TGG - chr1_1:71071289-71071308 None:intergenic 30.0%
GTTATATACAGTGAATCACT+AGG + chr1_1:71071317-71071336 Msa0040800:intron 30.0%
! AGTTTTTCCATTCAACGAAT+TGG - chr1_1:71071353-71071372 None:intergenic 30.0%
! GATTTTAAATGGAGAGGTAA+TGG - chr1_1:71071490-71071509 None:intergenic 30.0%
!! AAGGTTGATTTTAAATGGAG+AGG - chr1_1:71071496-71071515 None:intergenic 30.0%
!! AGGACAAGGTTGATTTTAAA+TGG - chr1_1:71071501-71071520 None:intergenic 30.0%
AGTTAATTGGCATATAGCTT+GGG - chr1_1:71071638-71071657 None:intergenic 30.0%
ATGACTATCATGCAGTTAAT+TGG - chr1_1:71071651-71071670 None:intergenic 30.0%
AAACTAGTAAGTAGCAATCA+TGG - chr1_1:71072010-71072029 None:intergenic 30.0%
!! GATTAGTAGCTTTAACTTTC+CGG + chr1_1:71072104-71072123 Msa0040800:intron 30.0%
GAAAGTCATTTCTCAAACTA+AGG - chr1_1:71072149-71072168 None:intergenic 30.0%
GTGAGAATGAGAAAGATATA+AGG - chr1_1:71072171-71072190 None:intergenic 30.0%
!!! CATTCTCACATTTTAACTTG+AGG + chr1_1:71072182-71072201 Msa0040800:intron 30.0%
TCTCTGAACCTTCTATAAAA+GGG - chr1_1:71072460-71072479 None:intergenic 30.0%
AAAGTCAACAAATTGGTTAG+AGG - chr1_1:71072538-71072557 None:intergenic 30.0%
TGAATTGGCAATTTCTCTAT+TGG + chr1_1:71072565-71072584 Msa0040800:intron 30.0%
!!! AGCAATGCTTTTTCAATAGT+AGG + chr1_1:71072620-71072639 Msa0040800:intron 30.0%
!!! AGAAACCTAATGTTTTTAGG+TGG + chr1_1:71072676-71072695 Msa0040800:intron 30.0%
AACTACCACCTAAAAACATT+AGG - chr1_1:71072684-71072703 None:intergenic 30.0%
TGAACAAGTGAATCAAATGT+TGG + chr1_1:71072743-71072762 Msa0040800:intron 30.0%
!!! ATTTTGATTGACACTGTTCT+TGG + chr1_1:71072976-71072995 Msa0040800:intron 30.0%
CTGTTCTTGGAAAATATCTT+TGG + chr1_1:71072989-71073008 Msa0040800:intron 30.0%
ATAAGTGCAGAACTAGTAAA+TGG - chr1_1:71073084-71073103 None:intergenic 30.0%
TCTGAACAAATAACATGAAC+AGG - chr1_1:71073212-71073231 None:intergenic 30.0%
ATAAACAAAGCAATGTGTAG+TGG + chr1_1:71073633-71073652 Msa0040800:CDS 30.0%
!! AACGATAGTGTTTTTCGATA+AGG - chr1_1:71073823-71073842 None:intergenic 30.0%
TTATCGAAAAACACTATCGT+TGG + chr1_1:71073822-71073841 Msa0040800:three_prime_UTR 30.0%
! TTTTGACCAAAACTTATGAG+AGG + chr1_1:71073914-71073933 Msa0040800:three_prime_UTR 30.0%
TTTGACCAAAACTTATGAGA+GGG + chr1_1:71073915-71073934 Msa0040800:three_prime_UTR 30.0%
TAGATCTCACATATACTCGA+AGG + chr1_1:71070389-71070408 Msa0040800:five_prime_UTR 35.0%
GAACAAAAGTTCAAAACGAC+AGG + chr1_1:71070425-71070444 Msa0040800:five_prime_UTR 35.0%
TTCAAAACGACAGGAATTAG+CGG + chr1_1:71070434-71070453 Msa0040800:five_prime_UTR 35.0%
AGGTCAGAAGAAATTATCGA+AGG + chr1_1:71070653-71070672 Msa0040800:intron 35.0%
!!! GTTTGTTCTCGTTGATTTTG+CGG - chr1_1:71070734-71070753 None:intergenic 35.0%
!! ATTGAAGGGTTTAGGTTGAA+AGG - chr1_1:71070779-71070798 None:intergenic 35.0%
TTTGTATGCTGCAGTTTAGA+AGG + chr1_1:71071094-71071113 Msa0040800:intron 35.0%
!! TAGAAGGTGCTATGCATATT+TGG + chr1_1:71071110-71071129 Msa0040800:intron 35.0%
! TGCATATTTGGTCTCTGTTT+TGG + chr1_1:71071122-71071141 Msa0040800:intron 35.0%
! GCATATTTGGTCTCTGTTTT+GGG + chr1_1:71071123-71071142 Msa0040800:intron 35.0%
GGGTTCGAAAATATTGACAA+TGG + chr1_1:71071228-71071247 Msa0040800:intron 35.0%
TATACAGTGAATCACTAGGA+TGG + chr1_1:71071321-71071340 Msa0040800:intron 35.0%
! TGGCTTGACATTTTCTCTAT+TGG + chr1_1:71071457-71071476 Msa0040800:intron 35.0%
TTTATTACCCCTTGTCAAGT+AGG - chr1_1:71071521-71071540 None:intergenic 35.0%
AGGGGTAATAAAAATTTCCC+TGG + chr1_1:71071529-71071548 Msa0040800:intron 35.0%
TTCAGCCCTTACAATACAAA+TGG - chr1_1:71071561-71071580 None:intergenic 35.0%
CTTGGGTTTGGAAAAATGTT+AGG - chr1_1:71071621-71071640 None:intergenic 35.0%
CAGTTAATTGGCATATAGCT+TGG - chr1_1:71071639-71071658 None:intergenic 35.0%
!! GTTTAGTGGATTTAGTTGCT+CGG + chr1_1:71071762-71071781 Msa0040800:intron 35.0%
!! TTTAGTGGATTTAGTTGCTC+GGG + chr1_1:71071763-71071782 Msa0040800:intron 35.0%
GACATGTGAAATTGGAACAT+GGG - chr1_1:71071901-71071920 None:intergenic 35.0%
TGACATGTGAAATTGGAACA+TGG - chr1_1:71071902-71071921 None:intergenic 35.0%
!! ATTTTTGAGAAAGCATGGCA+AGG + chr1_1:71071925-71071944 Msa0040800:intron 35.0%
!! TTTTTGAGAAAGCATGGCAA+GGG + chr1_1:71071926-71071945 Msa0040800:intron 35.0%
TCATTTACTTTGAATTCGGG+AGG - chr1_1:71071966-71071985 None:intergenic 35.0%
AAAACCATTTACCAATGGCA+AGG + chr1_1:71072059-71072078 Msa0040800:intron 35.0%
TCTCTATCACCATTTGTACT+TGG - chr1_1:71072339-71072358 None:intergenic 35.0%
TAATACAGTAGTGAACCCAA+GGG + chr1_1:71072381-71072400 Msa0040800:intron 35.0%
! TCATTGCTCCCTTTTATAGA+AGG + chr1_1:71072449-71072468 Msa0040800:intron 35.0%
CTCTCTGAACCTTCTATAAA+AGG - chr1_1:71072461-71072480 None:intergenic 35.0%
TCAGCTGATCCAATAATTCA+AGG - chr1_1:71072502-71072521 None:intergenic 35.0%
CAACAAATTGGTTAGAGGAA+AGG - chr1_1:71072533-71072552 None:intergenic 35.0%
AGGTTTGATATGGTACTGTA+TGG + chr1_1:71072640-71072659 Msa0040800:intron 35.0%
!! TTTTAGGTGGTAGTTAGTGA+TGG + chr1_1:71072689-71072708 Msa0040800:intron 35.0%
GTACATAACTCAATTCCTGA+TGG + chr1_1:71073551-71073570 Msa0040800:intron 35.0%
! TGCATTTTGCATCTTCTTCT+AGG + chr1_1:71073664-71073683 Msa0040800:three_prime_UTR 35.0%
TTGACCAAAACTTATGAGAG+GGG + chr1_1:71073916-71073935 Msa0040800:three_prime_UTR 35.0%
! AATTAGCGGAAAAGGGGTAT+AGG + chr1_1:71070448-71070467 Msa0040800:five_prime_UTR 40.0%
! ATTAGCGGAAAAGGGGTATA+GGG + chr1_1:71070449-71070468 Msa0040800:five_prime_UTR 40.0%
! TTTCTCATTATCGACTGCGA+TGG + chr1_1:71070535-71070554 Msa0040800:exon 40.0%
AGGTGATAGTACCAGACAAA+TGG + chr1_1:71070673-71070692 Msa0040800:CDS 40.0%
GTACCAGACAAATGGAAAGA+AGG + chr1_1:71070681-71070700 Msa0040800:CDS 40.0%
AACTCCTTTCCAAGAAGAAC+AGG + chr1_1:71070751-71070770 Msa0040800:CDS 40.0%
TGAAAGGAACCTGTTCTTCT+TGG - chr1_1:71070763-71070782 None:intergenic 40.0%
! TGCTACTCATGTTACTTCGT+TGG + chr1_1:71070993-71071012 Msa0040800:intron 40.0%
! GCTACTCATGTTACTTCGTT+GGG + chr1_1:71070994-71071013 Msa0040800:intron 40.0%
! CTACTCATGTTACTTCGTTG+GGG + chr1_1:71070995-71071014 Msa0040800:intron 40.0%
! AGGAAACAGTTGAGCTTGTT+AGG + chr1_1:71071033-71071052 Msa0040800:intron 40.0%
!!! GGTCTCTGTTTTGGGAATAT+TGG + chr1_1:71071131-71071150 Msa0040800:intron 40.0%
TCAACGTAGAGTCGACAAAT+TGG - chr1_1:71071437-71071456 None:intergenic 40.0%
!! ATTTGTCGACTCTACGTTGA+TGG + chr1_1:71071437-71071456 Msa0040800:intron 40.0%
AACCTTGTCCTACTTGACAA+GGG + chr1_1:71071510-71071529 Msa0040800:intron 40.0%
AGCCCACCATTTGTATTGTA+AGG + chr1_1:71071552-71071571 Msa0040800:intron 40.0%
GCCCACCATTTGTATTGTAA+GGG + chr1_1:71071553-71071572 Msa0040800:intron 40.0%
GCCCTTACAATACAAATGGT+GGG - chr1_1:71071557-71071576 None:intergenic 40.0%
AGCCCTTACAATACAAATGG+TGG - chr1_1:71071558-71071577 None:intergenic 40.0%
TATTGTAAGGGCTGAACATG+TGG + chr1_1:71071565-71071584 Msa0040800:intron 40.0%
ATTGTAAGGGCTGAACATGT+GGG + chr1_1:71071566-71071585 Msa0040800:intron 40.0%
!! TTGGCTTTGTACAGAGGATA+TGG + chr1_1:71071594-71071613 Msa0040800:intron 40.0%
ATTGGCATATAGCTTGGGTT+TGG - chr1_1:71071633-71071652 None:intergenic 40.0%
! GTACTTTGATGGCACATCAT+GGG - chr1_1:71071678-71071697 None:intergenic 40.0%
ATCATGGAACATGCACATGA+TGG - chr1_1:71071994-71072013 None:intergenic 40.0%
!! TAGTAGCTTTAACTTTCCGG+CGG + chr1_1:71072107-71072126 Msa0040800:intron 40.0%
TCTTTCAGATGGATCTCTTG+TGG - chr1_1:71072212-71072231 None:intergenic 40.0%
ATGGGAAGACCAAGTACAAA+TGG + chr1_1:71072327-71072346 Msa0040800:intron 40.0%
CTAATACAGTAGTGAACCCA+AGG + chr1_1:71072380-71072399 Msa0040800:intron 40.0%
AAATCCTCTCATTCTCCCTT+GGG - chr1_1:71072399-71072418 None:intergenic 40.0%
GCAATGATTGACATGGAAAG+TGG - chr1_1:71072436-71072455 None:intergenic 40.0%
AAAGGGAGCAATGATTGACA+TGG - chr1_1:71072443-71072462 None:intergenic 40.0%
TTACTCACAGAATCAAGTGC+TGG - chr1_1:71072775-71072794 None:intergenic 40.0%
ATTCTCCCATAACTTCACCA+TGG + chr1_1:71072835-71072854 Msa0040800:intron 40.0%
AGTGACCATGGTGAAGTTAT+GGG - chr1_1:71072843-71072862 None:intergenic 40.0%
AAGTGACCATGGTGAAGTTA+TGG - chr1_1:71072844-71072863 None:intergenic 40.0%
AACATCTCTCCAAACTGTCA+AGG + chr1_1:71073140-71073159 Msa0040800:intron 40.0%
! TCTCCAAACTGTCAAGGTTT+TGG + chr1_1:71073146-71073165 Msa0040800:intron 40.0%
! CTCCAAACTGTCAAGGTTTT+GGG + chr1_1:71073147-71073166 Msa0040800:intron 40.0%
AACCCAAAACCTTGACAGTT+TGG - chr1_1:71073152-71073171 None:intergenic 40.0%
CCACATGATGCAAAAATGCA+GGG - chr1_1:71073355-71073374 None:intergenic 40.0%
TCCACATGATGCAAAAATGC+AGG - chr1_1:71073356-71073375 None:intergenic 40.0%
TGCACTTAGTTGTTCCGTAA+GGG - chr1_1:71073415-71073434 None:intergenic 40.0%
ATGCACTTAGTTGTTCCGTA+AGG - chr1_1:71073416-71073435 None:intergenic 40.0%
!!! CTGATGGTTTTGTACTTGCA+GGG + chr1_1:71073567-71073586 Msa0040800:intron 40.0%
!!! TGATGGTTTTGTACTTGCAG+GGG + chr1_1:71073568-71073587 Msa0040800:intron 40.0%
!! ATACTCGAAGGCTGTCGTTT+TGG + chr1_1:71070401-71070420 Msa0040800:five_prime_UTR 45.0%
ACGACAGGAATTAGCGGAAA+AGG + chr1_1:71070440-71070459 Msa0040800:five_prime_UTR 45.0%
CGACAGGAATTAGCGGAAAA+GGG + chr1_1:71070441-71070460 Msa0040800:five_prime_UTR 45.0%
GACAGGAATTAGCGGAAAAG+GGG + chr1_1:71070442-71070461 Msa0040800:five_prime_UTR 45.0%
GCACCTTCTTTCCATTTGTC+TGG - chr1_1:71070687-71070706 None:intergenic 45.0%
GGTGCAAGCAATACTACTGA+AGG + chr1_1:71070702-71070721 Msa0040800:CDS 45.0%
GCAAGCAATACTACTGAAGG+CGG + chr1_1:71070705-71070724 Msa0040800:CDS 45.0%
GGAACCTGTTCTTCTTGGAA+AGG - chr1_1:71070758-71070777 None:intergenic 45.0%
! AAAGCACCAAACCAAGTACG+AGG - chr1_1:71071191-71071210 None:intergenic 45.0%
CAACCTTGTCCTACTTGACA+AGG + chr1_1:71071509-71071528 Msa0040800:intron 45.0%
ACCTTGTCCTACTTGACAAG+GGG + chr1_1:71071511-71071530 Msa0040800:intron 45.0%
!! AATACAAATGGTGGGCTCCA+GGG - chr1_1:71071549-71071568 None:intergenic 45.0%
TTGTAAGGGCTGAACATGTG+GGG + chr1_1:71071567-71071586 Msa0040800:intron 45.0%
! GGTACTTTGATGGCACATCA+TGG - chr1_1:71071679-71071698 None:intergenic 45.0%
TCGGGATCAAAACAGAAACG+TGG + chr1_1:71071781-71071800 Msa0040800:intron 45.0%
AGAGCCTTGCCATTGGTAAA+TGG - chr1_1:71072066-71072085 None:intergenic 45.0%
AGAGTAGCCAATTCGTGGAA+TGG + chr1_1:71072308-71072327 Msa0040800:intron 45.0%
GAGTAGCCAATTCGTGGAAT+GGG + chr1_1:71072309-71072328 Msa0040800:intron 45.0%
GAAATCCTCTCATTCTCCCT+TGG - chr1_1:71072400-71072419 None:intergenic 45.0%
GTAACGTGAAGAGGGAAACT+GGG - chr1_1:71072868-71072887 None:intergenic 45.0%
GAATGAGTGACGTTGTAGAC+TGG + chr1_1:71073174-71073193 Msa0040800:intron 45.0%
! CCCTGCATTTTTGCATCATG+TGG + chr1_1:71073352-71073371 Msa0040800:intron 45.0%
GCACTTAGTTGTTCCGTAAG+GGG - chr1_1:71073414-71073433 None:intergenic 45.0%
CACACGTGTGCTTACTCAAA+AGG + chr1_1:71073476-71073495 Msa0040800:CDS 45.0%
CCTGCAAGTACAAAACCATC+AGG - chr1_1:71073569-71073588 None:intergenic 45.0%
!!! CCTGATGGTTTTGTACTTGC+AGG + chr1_1:71073566-71073585 Msa0040800:intron 45.0%
ACCAAAACTTATGAGAGGGG+AGG + chr1_1:71073919-71073938 Msa0040800:three_prime_UTR 45.0%
! ACCTCCCCTCTCATAAGTTT+TGG - chr1_1:71073923-71073942 None:intergenic 45.0%
AACTTATGAGAGGGGAGGTT+TGG + chr1_1:71073924-71073943 Msa0040800:three_prime_UTR 45.0%
! GGAGGTTTGGTAGATGAATG+CGG + chr1_1:71073937-71073956 Msa0040800:three_prime_UTR 45.0%
AGCAATACTACTGAAGGCGG+TGG + chr1_1:71070708-71070727 Msa0040800:CDS 50.0%
! CTCATGTTACTTCGTTGGGG+AGG + chr1_1:71070998-71071017 Msa0040800:intron 50.0%
TGGGGAGGATCGATGTTCTT+AGG + chr1_1:71071013-71071032 Msa0040800:intron 50.0%
GTGGTGACATAATGCGTGCA+GGG - chr1_1:71071167-71071186 None:intergenic 50.0%
AGTGGTGACATAATGCGTGC+AGG - chr1_1:71071168-71071187 None:intergenic 50.0%
ATGTCACCACTCCTCGTACT+TGG + chr1_1:71071177-71071196 Msa0040800:intron 50.0%
ACCACTCCTCGTACTTGGTT+TGG + chr1_1:71071182-71071201 Msa0040800:intron 50.0%
ACCAAACCAAGTACGAGGAG+TGG - chr1_1:71071186-71071205 None:intergenic 50.0%
GTGAATCACTAGGATGGCAG+TGG + chr1_1:71071327-71071346 Msa0040800:intron 50.0%
GCAGTGGCCAATTCGTTGAA+TGG + chr1_1:71071343-71071362 Msa0040800:intron 50.0%
ACCCCTTGTCAAGTAGGACA+AGG - chr1_1:71071515-71071534 None:intergenic 50.0%
!! CAATACAAATGGTGGGCTCC+AGG - chr1_1:71071550-71071569 None:intergenic 50.0%
TACCAATGGCAAGGCTCTTC+AGG + chr1_1:71072068-71072087 Msa0040800:intron 50.0%
AACCTGAAGAGCCTTGCCAT+TGG - chr1_1:71072073-71072092 None:intergenic 50.0%
GGATGAGAGTAGCCAATTCG+TGG + chr1_1:71072303-71072322 Msa0040800:intron 50.0%
GGTCTTCCCATTCCACGAAT+TGG - chr1_1:71072318-71072337 None:intergenic 50.0%
TGAACCCAAGGGAGAATGAG+AGG + chr1_1:71072392-71072411 Msa0040800:intron 50.0%
GGAAACTGGGAAAGTGACCA+TGG - chr1_1:71072855-71072874 None:intergenic 50.0%
GGTAACGTGAAGAGGGAAAC+TGG - chr1_1:71072869-71072888 None:intergenic 50.0%
TGTCGAGGGTAACGTGAAGA+GGG - chr1_1:71072876-71072895 None:intergenic 50.0%
TTGTCGAGGGTAACGTGAAG+AGG - chr1_1:71072877-71072896 None:intergenic 50.0%
TCACATGTGTGCACATCGCT+TGG + chr1_1:71073040-71073059 Msa0040800:intron 50.0%
CACATGTGTGCACATCGCTT+GGG + chr1_1:71073041-71073060 Msa0040800:intron 50.0%
TGTGACAGTACTTGCCATCC+TGG - chr1_1:71073460-71073479 None:intergenic 50.0%
TAAGGGCTGAACATGTGGGG+AGG + chr1_1:71071570-71071589 Msa0040800:intron 55.0%
!! GGAGGCTTGGCTTTGTACAG+AGG + chr1_1:71071588-71071607 Msa0040800:intron 55.0%
GAATTCGGGAGGCAGTTTGC+AGG - chr1_1:71071955-71071974 None:intergenic 55.0%
CCTTGCAGATGGACCCCTTA+CGG + chr1_1:71073398-71073417 Msa0040800:intron 55.0%
TTGCAAGCAGCAAGTGCACC+AGG + chr1_1:71073439-71073458 Msa0040800:CDS 55.0%
AAGCAGCAAGTGCACCAGGA+TGG + chr1_1:71073443-71073462 Msa0040800:CDS 55.0%
GCTGAACATGTGGGGAGGCT+TGG + chr1_1:71071575-71071594 Msa0040800:intron 60.0%
CCGTAAGGGGTCCATCTGCA+AGG - chr1_1:71073401-71073420 None:intergenic 60.0%
TCCTACTTGCTCGCGTCCGC+CGG - chr1_1:71072126-71072145 None:intergenic 65.0%
GCAGGGGTCTGTGCGATGTG+TGG + chr1_1:71073584-71073603 Msa0040800:intron 65.0%
TCCGGCGGACGCGAGCAAGT+AGG + chr1_1:71072122-71072141 Msa0040800:intron 70.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 71070365 71074005 71070365 ID=Msa0040800;Name=Msa0040800
chr1_1 mRNA 71070365 71074005 71070365 ID=Msa0040800-mRNA-1;Parent=Msa0040800;Name=Msa0040800-mRNA-1;_AED=0.00;_eAED=0.00;_QI=189|1|1|1|0.66|0.75|4|353|96
chr1_1 exon 71070365 71070569 71070365 ID=Msa0040800-mRNA-1:exon:23472;Parent=Msa0040800-mRNA-1
chr1_1 exon 71070655 71070772 71070655 ID=Msa0040800-mRNA-1:exon:23473;Parent=Msa0040800-mRNA-1
chr1_1 exon 71073406 71073497 71073406 ID=Msa0040800-mRNA-1:exon:23474;Parent=Msa0040800-mRNA-1
chr1_1 exon 71073588 71074005 71073588 ID=Msa0040800-mRNA-1:exon:23475;Parent=Msa0040800-mRNA-1
chr1_1 five_prime_UTR 71070365 71070553 71070365 ID=Msa0040800-mRNA-1:five_prime_utr;Parent=Msa0040800-mRNA-1
chr1_1 CDS 71070554 71070569 71070554 ID=Msa0040800-mRNA-1:cds;Parent=Msa0040800-mRNA-1
chr1_1 CDS 71070655 71070772 71070655 ID=Msa0040800-mRNA-1:cds;Parent=Msa0040800-mRNA-1
chr1_1 CDS 71073406 71073497 71073406 ID=Msa0040800-mRNA-1:cds;Parent=Msa0040800-mRNA-1
chr1_1 CDS 71073588 71073652 71073588 ID=Msa0040800-mRNA-1:cds;Parent=Msa0040800-mRNA-1
chr1_1 three_prime_UTR 71073653 71074005 71073653 ID=Msa0040800-mRNA-1:three_prime_utr;Parent=Msa0040800-mRNA-1
Gene Sequence

>Msa0040800

ATGGTCTGCGAAAAGTGTCAGAAGAAATTATCGAAGGTGATAGTACCAGACAAATGGAAAGAAGGTGCAAGCAATACTACTGAAGGCGGTGGCCGCAAAATCAACGAGAACAAACTCCTTTCCAAGAAGAACAGATGGACCCCTTACGGAACAACTAAGTGCATCATTTGCAAGCAGCAAGTGCACCAGGATGGCAAGTACTGTCACACGTGTGCTTACTCAAAAGGGGTCTGTGCGATGTGTGGCAAGCAAGTTCTTGATACCAAGTTTTATAAACAAAGCAATGTGTAG
Protein sequence

>Msa0040800

MVCEKCQKKLSKVIVPDKWKEGASNTTEGGGRKINENKLLSKKNRWTPYGTTKCIICKQQVHQDGKYCHTCAYSKGVCAMCGKQVLDTKFYKQSNV*