Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0045140 | Msa0045180 | 0.825655 | 4.163069e-54 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0045180 | MtrunA17_Chr2g0301881 | 79.487 | 39 | 8 | 0 | 15 | 53 | 304 | 342 | 2.21e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 14 sgRNAs with CRISPR-Local
Find 102 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTCTTGGTCGTGCTTATTT+TGG | 0.178155 | 1_1:-76880474 | Msa0045180:CDS |
| TCTTTATTACTGTTTGTTCT+TGG | 0.257547 | 1_1:-76880570 | Msa0045180:CDS |
| ATGAAATCACTTGCAAAAGA+TGG | 0.438991 | 1_1:+76881382 | None:intergenic |
| TAGTCAATGAAGACAGCGTT+TGG | 0.456859 | 1_1:-76880594 | Msa0045180:CDS |
| CACTTGCAAAAGATGGAGAC+AGG | 0.478454 | 1_1:+76881389 | None:intergenic |
| ATGCAGGCGAGGTGCTGTCT+CGG | 0.514779 | 1_1:+76881351 | None:intergenic |
| AGAGCTTGGTGTAGTCCTCT+TGG | 0.517761 | 1_1:-76880489 | Msa0045180:CDS |
| CCTTCATTAGCTCTTCATGC+AGG | 0.532164 | 1_1:+76881335 | None:intergenic |
| GTGCAACATACATTTAACCT+TGG | 0.533755 | 1_1:-76880429 | Msa0045180:CDS |
| CCTGCATGAAGAGCTAATGA+AGG | 0.605404 | 1_1:-76881335 | Msa0045180:intron |
| ATTAGCTCTTCATGCAGGCG+AGG | 0.608084 | 1_1:+76881340 | None:intergenic |
| CCAAAATAAGCACGACCAAG+AGG | 0.622212 | 1_1:+76880474 | None:intergenic |
| ACTTGCAAAAGATGGAGACA+GGG | 0.688727 | 1_1:+76881390 | None:intergenic |
| AATTCTAGAGTGATATACCA+AGG | 0.688891 | 1_1:+76880412 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTATATGATATGTTTTAAT+AGG | - | chr1_1:76880880-76880899 | Msa0045180:intron | 10.0% |
| !!! | TTTATATGATATGTTTTAAT+AGG | - | chr1_1:76880880-76880899 | Msa0045180:intron | 10.0% |
| !! | CAAAAAAAGAATACATTATT+TGG | + | chr1_1:76880471-76880490 | None:intergenic | 15.0% |
| !!! | TATTTTACTTATTGAAAAAC+TGG | - | chr1_1:76880779-76880798 | Msa0045180:intron | 15.0% |
| !!! | ATTTTACTTATTGAAAAACT+GGG | - | chr1_1:76880780-76880799 | Msa0045180:intron | 15.0% |
| !!! | AAACTACATAAATTTAAAGT+AGG | - | chr1_1:76880815-76880834 | Msa0045180:intron | 15.0% |
| !! | TGATAATCTTAATTTAAATG+TGG | + | chr1_1:76880974-76880993 | None:intergenic | 15.0% |
| !! | CAAAAAAAGAATACATTATT+TGG | + | chr1_1:76880471-76880490 | None:intergenic | 15.0% |
| !!! | TATTTTACTTATTGAAAAAC+TGG | - | chr1_1:76880779-76880798 | Msa0045180:intron | 15.0% |
| !!! | ATTTTACTTATTGAAAAACT+GGG | - | chr1_1:76880780-76880799 | Msa0045180:intron | 15.0% |
| !!! | AAACTACATAAATTTAAAGT+AGG | - | chr1_1:76880815-76880834 | Msa0045180:intron | 15.0% |
| !! | TGATAATCTTAATTTAAATG+TGG | + | chr1_1:76880974-76880993 | None:intergenic | 15.0% |
| ! | TCTAAACGAAAATATCATAG+GGG | + | chr1_1:76880540-76880559 | None:intergenic | 25.0% |
| ! | GTCTAAACGAAAATATCATA+GGG | + | chr1_1:76880541-76880560 | None:intergenic | 25.0% |
| ! | TGTCTAAACGAAAATATCAT+AGG | + | chr1_1:76880542-76880561 | None:intergenic | 25.0% |
| ! | ATCGAATCTAATCTATCAAA+AGG | + | chr1_1:76880710-76880729 | None:intergenic | 25.0% |
| ! | CATGAAATATATAAAGAGAC+TGG | + | chr1_1:76881102-76881121 | None:intergenic | 25.0% |
| ! | AAAGAAATTGTTGTAGATGA+AGG | + | chr1_1:76881213-76881232 | None:intergenic | 25.0% |
| !! | TATGATTTTTCTCTGAACTT+TGG | - | chr1_1:76881335-76881354 | Msa0045180:intron | 25.0% |
| !! | TCATTGACTAGTAAATTGAA+AGG | + | chr1_1:76881390-76881409 | None:intergenic | 25.0% |
| ! | TCTTTATTACTGTTTGTTCT+TGG | - | chr1_1:76881424-76881443 | Msa0045180:CDS | 25.0% |
| ! | TCTAAACGAAAATATCATAG+GGG | + | chr1_1:76880540-76880559 | None:intergenic | 25.0% |
| ! | GTCTAAACGAAAATATCATA+GGG | + | chr1_1:76880541-76880560 | None:intergenic | 25.0% |
| ! | TGTCTAAACGAAAATATCAT+AGG | + | chr1_1:76880542-76880561 | None:intergenic | 25.0% |
| ! | ATCGAATCTAATCTATCAAA+AGG | + | chr1_1:76880710-76880729 | None:intergenic | 25.0% |
| ! | CATGAAATATATAAAGAGAC+TGG | + | chr1_1:76881102-76881121 | None:intergenic | 25.0% |
| ! | AAAGAAATTGTTGTAGATGA+AGG | + | chr1_1:76881213-76881232 | None:intergenic | 25.0% |
| !! | TATGATTTTTCTCTGAACTT+TGG | - | chr1_1:76881335-76881354 | Msa0045180:intron | 25.0% |
| !! | TCATTGACTAGTAAATTGAA+AGG | + | chr1_1:76881390-76881409 | None:intergenic | 25.0% |
| ! | TCTTTATTACTGTTTGTTCT+TGG | - | chr1_1:76881424-76881443 | Msa0045180:CDS | 25.0% |
| ATGAAATCACTTGCAAAAGA+TGG | + | chr1_1:76880615-76880634 | None:intergenic | 30.0% | |
| ! | CTTTTGCAAGTGATTTCATA+CGG | - | chr1_1:76880616-76880635 | Msa0045180:intron | 30.0% |
| ATACTTAACAGTAAGACGAA+CGG | + | chr1_1:76880754-76880773 | None:intergenic | 30.0% | |
| !! | GTGGTTATCATCTTTAGTTT+TGG | + | chr1_1:76880955-76880974 | None:intergenic | 30.0% |
| GTCATCATTATCTTATGTGA+TGG | - | chr1_1:76881004-76881023 | Msa0045180:intron | 30.0% | |
| TAAATGCAAGCGATAAGAAA+AGG | + | chr1_1:76881187-76881206 | None:intergenic | 30.0% | |
| ! | ATTTCTTTTCCTATGCAACT+AGG | - | chr1_1:76881225-76881244 | Msa0045180:intron | 30.0% |
| TGTAAATCAATTGTTGTACC+AGG | - | chr1_1:76881248-76881267 | Msa0045180:intron | 30.0% | |
| GTAAATCAATTGTTGTACCA+GGG | - | chr1_1:76881249-76881268 | Msa0045180:intron | 30.0% | |
| ! | GCACATTTTCTATAACTTCT+TGG | + | chr1_1:76881549-76881568 | None:intergenic | 30.0% |
| ATGAAATCACTTGCAAAAGA+TGG | + | chr1_1:76880615-76880634 | None:intergenic | 30.0% | |
| ! | CTTTTGCAAGTGATTTCATA+CGG | - | chr1_1:76880616-76880635 | Msa0045180:intron | 30.0% |
| ATACTTAACAGTAAGACGAA+CGG | + | chr1_1:76880754-76880773 | None:intergenic | 30.0% | |
| !! | GTGGTTATCATCTTTAGTTT+TGG | + | chr1_1:76880955-76880974 | None:intergenic | 30.0% |
| GTCATCATTATCTTATGTGA+TGG | - | chr1_1:76881004-76881023 | Msa0045180:intron | 30.0% | |
| TAAATGCAAGCGATAAGAAA+AGG | + | chr1_1:76881187-76881206 | None:intergenic | 30.0% | |
| ! | ATTTCTTTTCCTATGCAACT+AGG | - | chr1_1:76881225-76881244 | Msa0045180:intron | 30.0% |
| TGTAAATCAATTGTTGTACC+AGG | - | chr1_1:76881248-76881267 | Msa0045180:intron | 30.0% | |
| GTAAATCAATTGTTGTACCA+GGG | - | chr1_1:76881249-76881268 | Msa0045180:intron | 30.0% | |
| ! | GCACATTTTCTATAACTTCT+TGG | + | chr1_1:76881549-76881568 | None:intergenic | 30.0% |
| TCAACAGTTGCTAAAGTAAG+AGG | - | chr1_1:76881141-76881160 | Msa0045180:intron | 35.0% | |
| CAACAGTTGCTAAAGTAAGA+GGG | - | chr1_1:76881142-76881161 | Msa0045180:intron | 35.0% | |
| TGATTTACACCTAGTTGCAT+AGG | + | chr1_1:76881237-76881256 | None:intergenic | 35.0% | |
| ! | TTTTGTTGATACAGAGAGCT+TGG | - | chr1_1:76881491-76881510 | Msa0045180:intron | 35.0% |
| GTGCAACATACATTTAACCT+TGG | - | chr1_1:76881565-76881584 | Msa0045180:CDS | 35.0% | |
| TCAACAGTTGCTAAAGTAAG+AGG | - | chr1_1:76881141-76881160 | Msa0045180:intron | 35.0% | |
| CAACAGTTGCTAAAGTAAGA+GGG | - | chr1_1:76881142-76881161 | Msa0045180:intron | 35.0% | |
| TGATTTACACCTAGTTGCAT+AGG | + | chr1_1:76881237-76881256 | None:intergenic | 35.0% | |
| ! | TTTTGTTGATACAGAGAGCT+TGG | - | chr1_1:76881491-76881510 | Msa0045180:intron | 35.0% |
| GTGCAACATACATTTAACCT+TGG | - | chr1_1:76881565-76881584 | Msa0045180:CDS | 35.0% | |
| ACTTGCAAAAGATGGAGACA+GGG | + | chr1_1:76880607-76880626 | None:intergenic | 40.0% | |
| TAGTCAATGAAGACAGCGTT+TGG | - | chr1_1:76881400-76881419 | Msa0045180:CDS | 40.0% | |
| ACTTGCAAAAGATGGAGACA+GGG | + | chr1_1:76880607-76880626 | None:intergenic | 40.0% | |
| TAGTCAATGAAGACAGCGTT+TGG | - | chr1_1:76881400-76881419 | Msa0045180:CDS | 40.0% | |
| TCATAGGGGTGAGCACAAAA+AGG | + | chr1_1:76880526-76880545 | None:intergenic | 45.0% | |
| CACTTGCAAAAGATGGAGAC+AGG | + | chr1_1:76880608-76880627 | None:intergenic | 45.0% | |
| ! | CCTTCATTAGCTCTTCATGC+AGG | + | chr1_1:76880662-76880681 | None:intergenic | 45.0% |
| CCTGCATGAAGAGCTAATGA+AGG | - | chr1_1:76880659-76880678 | Msa0045180:intron | 45.0% | |
| GAAGAGCTAATGAAGGTGAC+TGG | - | chr1_1:76880666-76880685 | Msa0045180:intron | 45.0% | |
| ATATAAACCCACACTCCCAC+AGG | + | chr1_1:76880867-76880886 | None:intergenic | 45.0% | |
| ! | GACTGGAGCATTAGACAATG+AGG | + | chr1_1:76881085-76881104 | None:intergenic | 45.0% |
| ATTCGCATTGCTTCAAACCC+TGG | + | chr1_1:76881269-76881288 | None:intergenic | 45.0% | |
| CCAAAATAAGCACGACCAAG+AGG | + | chr1_1:76881523-76881542 | None:intergenic | 45.0% | |
| ! | CCTCTTGGTCGTGCTTATTT+TGG | - | chr1_1:76881520-76881539 | Msa0045180:intron | 45.0% |
| !!! | CTTGGTCGTGCTTATTTTGG+TGG | - | chr1_1:76881523-76881542 | Msa0045180:intron | 45.0% |
| TCATAGGGGTGAGCACAAAA+AGG | + | chr1_1:76880526-76880545 | None:intergenic | 45.0% | |
| CACTTGCAAAAGATGGAGAC+AGG | + | chr1_1:76880608-76880627 | None:intergenic | 45.0% | |
| ! | CCTTCATTAGCTCTTCATGC+AGG | + | chr1_1:76880662-76880681 | None:intergenic | 45.0% |
| CCTGCATGAAGAGCTAATGA+AGG | - | chr1_1:76880659-76880678 | Msa0045180:intron | 45.0% | |
| GAAGAGCTAATGAAGGTGAC+TGG | - | chr1_1:76880666-76880685 | Msa0045180:intron | 45.0% | |
| ATATAAACCCACACTCCCAC+AGG | + | chr1_1:76880867-76880886 | None:intergenic | 45.0% | |
| ! | GACTGGAGCATTAGACAATG+AGG | + | chr1_1:76881085-76881104 | None:intergenic | 45.0% |
| ATTCGCATTGCTTCAAACCC+TGG | + | chr1_1:76881269-76881288 | None:intergenic | 45.0% | |
| CCAAAATAAGCACGACCAAG+AGG | + | chr1_1:76881523-76881542 | None:intergenic | 45.0% | |
| ! | CCTCTTGGTCGTGCTTATTT+TGG | - | chr1_1:76881520-76881539 | Msa0045180:intron | 45.0% |
| !!! | CTTGGTCGTGCTTATTTTGG+TGG | - | chr1_1:76881523-76881542 | Msa0045180:intron | 45.0% |
| ! | ATTAGCTCTTCATGCAGGCG+AGG | + | chr1_1:76880657-76880676 | None:intergenic | 50.0% |
| AGTAGGCTGAACTGCACTTG+TGG | - | chr1_1:76880832-76880851 | Msa0045180:intron | 50.0% | |
| TTGTGGCAAGCTCTTCCTGT+GGG | - | chr1_1:76880849-76880868 | Msa0045180:intron | 50.0% | |
| !! | AGAGCTTGGTGTAGTCCTCT+TGG | - | chr1_1:76881505-76881524 | Msa0045180:intron | 50.0% |
| ! | ATTAGCTCTTCATGCAGGCG+AGG | + | chr1_1:76880657-76880676 | None:intergenic | 50.0% |
| AGTAGGCTGAACTGCACTTG+TGG | - | chr1_1:76880832-76880851 | Msa0045180:intron | 50.0% | |
| TTGTGGCAAGCTCTTCCTGT+GGG | - | chr1_1:76880849-76880868 | Msa0045180:intron | 50.0% | |
| !! | AGAGCTTGGTGTAGTCCTCT+TGG | - | chr1_1:76881505-76881524 | Msa0045180:intron | 50.0% |
| CTTGTGGCAAGCTCTTCCTG+TGG | - | chr1_1:76880848-76880867 | Msa0045180:intron | 55.0% | |
| AAGCTCTTCCTGTGGGAGTG+TGG | - | chr1_1:76880856-76880875 | Msa0045180:intron | 55.0% | |
| ! | AGCTCTTCCTGTGGGAGTGT+GGG | - | chr1_1:76880857-76880876 | Msa0045180:intron | 55.0% |
| CTTGTGGCAAGCTCTTCCTG+TGG | - | chr1_1:76880848-76880867 | Msa0045180:intron | 55.0% | |
| AAGCTCTTCCTGTGGGAGTG+TGG | - | chr1_1:76880856-76880875 | Msa0045180:intron | 55.0% | |
| ! | AGCTCTTCCTGTGGGAGTGT+GGG | - | chr1_1:76880857-76880876 | Msa0045180:intron | 55.0% |
| !! | ATGCAGGCGAGGTGCTGTCT+CGG | + | chr1_1:76880646-76880665 | None:intergenic | 60.0% |
| !! | ATGCAGGCGAGGTGCTGTCT+CGG | + | chr1_1:76880646-76880665 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_1 | gene | 76880416 | 76881600 | 76880416 | ID=Msa0045180;Name=Msa0045180 |
| chr1_1 | mRNA | 76880416 | 76881600 | 76880416 | ID=Msa0045180-mRNA-1;Parent=Msa0045180;Name=Msa0045180-mRNA-1;_AED=0.63;_eAED=0.81;_QI=0|0|0|0.66|0|0.33|3|0|118 |
| chr1_1 | exon | 76881564 | 76881600 | 76881564 | ID=Msa0045180-mRNA-1:exon:26262;Parent=Msa0045180-mRNA-1 |
| chr1_1 | exon | 76881336 | 76881457 | 76881336 | ID=Msa0045180-mRNA-1:exon:26261;Parent=Msa0045180-mRNA-1 |
| chr1_1 | exon | 76880416 | 76880613 | 76880416 | ID=Msa0045180-mRNA-1:exon:26260;Parent=Msa0045180-mRNA-1 |
| chr1_1 | CDS | 76881564 | 76881600 | 76881564 | ID=Msa0045180-mRNA-1:cds;Parent=Msa0045180-mRNA-1 |
| chr1_1 | CDS | 76881336 | 76881457 | 76881336 | ID=Msa0045180-mRNA-1:cds;Parent=Msa0045180-mRNA-1 |
| chr1_1 | CDS | 76880416 | 76880613 | 76880416 | ID=Msa0045180-mRNA-1:cds;Parent=Msa0045180-mRNA-1 |
| Gene Sequence |
| Protein sequence |
