AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0052430

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 73 sgRNAs with CRISPR-Local

Find 240 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTGCAGGCTACTTTAGT+TGG 0.267777 1_2:-6671131 Msa0052430:intron
TTATGGATTACTCTCTTTGA+TGG 0.288483 1_2:-6670922 Msa0052430:three_prime_UTR
TGTAATCTTCCTATGGCATT+TGG 0.337813 1_2:-6671008 Msa0052430:three_prime_UTR
TCACATATCTCTAGAATTCA+TGG 0.348342 1_2:-6674604 Msa0052430:exon
TGCACATTTATCTTTATTGT+AGG 0.367026 1_2:-6671043 Msa0052430:CDS
TAACTTAATATAAAAGTAGA+TGG 0.386238 1_2:+6675012 None:intergenic
GCGGCTAGGGTTTGTTGCAC+GGG 0.394480 1_2:+6675114 None:intergenic
ACAAAATCTTGTATTATATG+AGG 0.407467 1_2:-6670729 Msa0052430:three_prime_UTR
TATACGATGGCCGAGATCTA+AGG 0.413093 1_2:+6674943 None:intergenic
TTCTGCAGGCTACTTTAGTT+GGG 0.427760 1_2:-6671130 Msa0052430:intron
GGCGGCTAGGGTTTGTTGCA+CGG 0.437937 1_2:+6675113 None:intergenic
TAGCCGCCGTACACTGTTCA+TGG 0.442024 1_2:-6675098 Msa0052430:five_prime_UTR
ATTTCCAGCACGGTGGTCAC+CGG 0.452137 1_2:+6674832 None:intergenic
ATTACCTCATTATTATATGA+AGG 0.457777 1_2:+6671475 None:intergenic
CACCAGCCAGGGATGATTCA+AGG 0.462778 1_2:-6674041 Msa0052430:CDS
ATTTGTTCATCAAATGCAAT+GGG 0.468988 1_2:-6671105 Msa0052430:CDS
TGAGGAAGGTATATGTGGGC+TGG 0.476037 1_2:-6670711 Msa0052430:three_prime_UTR
CCATGTCTCCATCACCAGCC+AGG 0.485470 1_2:-6674053 Msa0052430:CDS
GAATCATCCCTGGCTGGTGA+TGG 0.498880 1_2:+6674045 None:intergenic
ATGAACAGTGTACGGCGGCT+AGG 0.501930 1_2:+6675100 None:intergenic
CATACGTTTCCAACCGCCCT+TGG 0.508531 1_2:-6675069 Msa0052430:five_prime_UTR
TTTCCCGGTGACCACCGTGC+TGG 0.509645 1_2:-6674836 Msa0052430:five_prime_UTR
TATTTGTTCATCAAATGCAA+TGG 0.509786 1_2:-6671106 Msa0052430:CDS
TGAACAGTGTACGGCGGCTA+GGG 0.510818 1_2:+6675101 None:intergenic
GAGGAAGGTATATGTGGGCT+GGG 0.517026 1_2:-6670710 Msa0052430:three_prime_UTR
CTCCTCTACAAAACCCTCAA+AGG 0.517319 1_2:-6674877 Msa0052430:five_prime_UTR
GGAAATCACCATCGCCGTAC+GGG 0.517956 1_2:-6674815 Msa0052430:five_prime_UTR
AACCTTGAATCATCCCTGGC+TGG 0.520221 1_2:+6674039 None:intergenic
GTGTAGAGGAAAGTAAAGAT+TGG 0.524707 1_2:+6671081 None:intergenic
TCTAGAGATATGTGAAGTGA+TGG 0.525699 1_2:+6674612 None:intergenic
CTCAAACCTTGAATCATCCC+TGG 0.531445 1_2:+6674035 None:intergenic
TTTCCAGCACGGTGGTCACC+GGG 0.532021 1_2:+6674833 None:intergenic
TTGCTTGCAGTAGATCCGTC+AGG 0.533615 1_2:-6674573 Msa0052430:intron
AGGGCGGTTGGAAACGTATG+AGG 0.540377 1_2:+6675072 None:intergenic
AAGCACTTGAGATGAGAGGT+AGG 0.543848 1_2:+6674640 None:intergenic
AGTGATTTCCCGTACGGCGA+TGG 0.545931 1_2:+6674807 None:intergenic
TTATATGAGGAAGGTATATG+TGG 0.550491 1_2:-6670716 Msa0052430:three_prime_UTR
TGACTGATGTAATCTTCCTA+TGG 0.552331 1_2:-6671015 Msa0052430:exon
GGAGAGGATGACGACGGTGA+TGG 0.555396 1_2:+6675038 None:intergenic
TAATATAAAAGTAGATGGAA+AGG 0.557946 1_2:+6675017 None:intergenic
GTGGCATAACCAAGGGCGGT+TGG 0.558898 1_2:+6675060 None:intergenic
TCATCTTCTACCTTAGATCT+CGG 0.566041 1_2:-6674953 Msa0052430:five_prime_UTR
TGGAAATCACCATCGCCGTA+CGG 0.566630 1_2:-6674816 Msa0052430:five_prime_UTR
GGAAATCACTTCCACCGTGA+CGG 0.571968 1_2:-6674794 Msa0052430:five_prime_UTR
TGGATTACTCTCTTTGATGG+AGG 0.573975 1_2:-6670919 Msa0052430:three_prime_UTR
TTTCAAAATGAGATATACGA+TGG 0.585630 1_2:+6674930 None:intergenic
AGGTAGCCATGAACAGTGTA+CGG 0.586368 1_2:+6675092 None:intergenic
CCTCAAAGGAAAATCATGAT+CGG 0.587722 1_2:-6674863 Msa0052430:five_prime_UTR
CGGCTAGGGTTTGTTGCACG+GGG 0.591579 1_2:+6675115 None:intergenic
CATGTCTCCATCACCAGCCA+GGG 0.592098 1_2:-6674052 Msa0052430:CDS
AATCTTGTATTATATGAGGA+AGG 0.593918 1_2:-6670725 Msa0052430:three_prime_UTR
GAGGATGACGACGGTGATGG+TGG 0.600793 1_2:+6675041 None:intergenic
GAAGCTAATCCAAATGCCAT+AGG 0.601194 1_2:+6670999 None:intergenic
CCTGGCTGGTGATGGAGACA+TGG 0.601882 1_2:+6674053 None:intergenic
GAAGAAGCACTTGAGATGAG+AGG 0.603758 1_2:+6674636 None:intergenic
ATAAAGATAAATGTGCATCA+AGG 0.605215 1_2:+6671049 None:intergenic
TATATGAGGAAGGTATATGT+GGG 0.606959 1_2:-6670715 Msa0052430:three_prime_UTR
TCAGCCTTCATATAATAATG+AGG 0.609329 1_2:-6671479 Msa0052430:intron
CGGTGATGGTGGCATAACCA+AGG 0.615381 1_2:+6675052 None:intergenic
GGTGGAAGTGATTTCCCGTA+CGG 0.618841 1_2:+6674801 None:intergenic
GGTGATGGTGGCATAACCAA+GGG 0.629833 1_2:+6675053 None:intergenic
TAAAAGTAGATGGAAAGGAG+AGG 0.631548 1_2:+6675022 None:intergenic
CAAGGACATCAAGTGTGTAG+AGG 0.631925 1_2:+6671067 None:intergenic
GGATCTACTGCAAGCAACAA+CGG 0.639621 1_2:+6674579 None:intergenic
GGCGATGGTGATTTCCAGCA+CGG 0.643077 1_2:+6674822 None:intergenic
AGATCTAAGGTAGAAGATGA+CGG 0.648460 1_2:+6674956 None:intergenic
TGGTGATGGAGACATGGAGT+AGG 0.658191 1_2:+6674059 None:intergenic
ACAGTAGCGTTATTCCGTCA+CGG 0.667037 1_2:+6674780 None:intergenic
GATGGTGGCATAACCAAGGG+CGG 0.678558 1_2:+6675056 None:intergenic
TGGAAAGGAGAGGATGACGA+CGG 0.719635 1_2:+6675032 None:intergenic
GATGGTGATTTCCAGCACGG+TGG 0.738379 1_2:+6674825 None:intergenic
TAGCCATGAACAGTGTACGG+CGG 0.781625 1_2:+6675095 None:intergenic
GTAGCGTTATTCCGTCACGG+TGG 0.813893 1_2:+6674783 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGTTCTATTAATTTTTATTT+TGG - chr1_2:6671127-6671146 Msa0052430:intron 10.0%
!!! TTTTTTAAAATACCAAAAAA+TGG + chr1_2:6671407-6671426 None:intergenic 10.0%
!!! AAACTGTTAAATTTAAAATA+GGG + chr1_2:6672472-6672491 None:intergenic 10.0%
!!! AAAACTGTTAAATTTAAAAT+AGG + chr1_2:6672473-6672492 None:intergenic 10.0%
!!! TATTTTAAATTTAACAGTTT+TGG - chr1_2:6672472-6672491 Msa0052430:intron 10.0%
!!! TTAATTTGTTTATTAGATAT+TGG + chr1_2:6673550-6673569 None:intergenic 10.0%
!! TAACTTAATATAAAAGTAGA+TGG + chr1_2:6670832-6670851 None:intergenic 15.0%
!!! TTTCTTTTAAACAAAAATTG+TGG - chr1_2:6671471-6671490 Msa0052430:intron 15.0%
!! ATATAAACAAAAATGACAAA+AGG + chr1_2:6671618-6671637 None:intergenic 15.0%
!!! TTTTGTTTATATCCTATTAT+AGG - chr1_2:6671626-6671645 Msa0052430:intron 15.0%
!! ATTTACATTATTAAGACATT+AGG + chr1_2:6671661-6671680 None:intergenic 15.0%
!! TGTCTTAATAATGTAAATTT+AGG - chr1_2:6671663-6671682 Msa0052430:intron 15.0%
!! GAAAACTAAAATAAACATAA+GGG + chr1_2:6672339-6672358 None:intergenic 15.0%
!! TGAAAACTAAAATAAACATA+AGG + chr1_2:6672340-6672359 None:intergenic 15.0%
!!! AACTGTTAAATTTAAAATAG+GGG + chr1_2:6672471-6672490 None:intergenic 15.0%
!!! ATATTTTAGACAATATTTTG+TGG + chr1_2:6672503-6672522 None:intergenic 15.0%
!!! ATTTTAGTTCAAAAATATGT+AGG - chr1_2:6672641-6672660 Msa0052430:intron 15.0%
!!! TTTTAGTTCAAAAATATGTA+GGG - chr1_2:6672642-6672661 Msa0052430:intron 15.0%
!! TCTTAAGAAAAAAGTTAAAT+TGG - chr1_2:6672783-6672802 Msa0052430:intron 15.0%
!!! TTAAAAGGGATTATTTTTAA+TGG - chr1_2:6673363-6673382 Msa0052430:intron 15.0%
!!! AAATAATCATCCTAAATTTT+AGG - chr1_2:6673453-6673472 Msa0052430:intron 15.0%
!!! TTTTTTGTCATAAGAATAAT+TGG - chr1_2:6673939-6673958 Msa0052430:intron 15.0%
!!! TAGTTTTATTCTTTCTTTAT+TGG - chr1_2:6674428-6674447 Msa0052430:intron 15.0%
!!! AATATTGAGTTTTTCTAATT+TGG - chr1_2:6674528-6674547 Msa0052430:intron 15.0%
!! AAGATAATGTCAAAAAATTA+TGG - chr1_2:6674902-6674921 Msa0052430:five_prime_UTR 15.0%
!!! TTGATCATTTTTTACTATAT+TGG - chr1_2:6675059-6675078 Msa0052430:five_prime_UTR 15.0%
!! TAATATAAAAGTAGATGGAA+AGG + chr1_2:6670827-6670846 None:intergenic 20.0%
!!! AGAAAAAGATTTTCTTCATA+TGG + chr1_2:6671296-6671315 None:intergenic 20.0%
!!! TTACTTTTATCTGATTTTCA+AGG - chr1_2:6671737-6671756 Msa0052430:intron 20.0%
!! AAAATATAGTAATATGATCG+AGG + chr1_2:6671860-6671879 None:intergenic 20.0%
!!! CTGATCATTAATATCTATTT+TGG - chr1_2:6672295-6672314 Msa0052430:intron 20.0%
!!! GTTTATTTTAGTTTTCAAGT+TGG - chr1_2:6672343-6672362 Msa0052430:intron 20.0%
!!! TTGAAACTTCTAAAACATAA+GGG + chr1_2:6672370-6672389 None:intergenic 20.0%
!!! TTGAAACTTTTAAAACGTAA+GGG + chr1_2:6672399-6672418 None:intergenic 20.0%
!! ATTTCAAAATCTTACAGTTT+CGG + chr1_2:6672669-6672688 None:intergenic 20.0%
!!! AAACTGTAAGATTTTGAAAT+AGG - chr1_2:6672669-6672688 Msa0052430:intron 20.0%
!!! AACTGTAAGATTTTGAAATA+GGG - chr1_2:6672670-6672689 Msa0052430:intron 20.0%
!! TATTTAACCAACCAATATTA+GGG + chr1_2:6672744-6672763 None:intergenic 20.0%
!! ATATTTAACCAACCAATATT+AGG + chr1_2:6672745-6672764 None:intergenic 20.0%
!!! TTTTTTTTGTCTAAATTGTG+AGG - chr1_2:6672848-6672867 Msa0052430:intron 20.0%
!! TCTACTTAAATCATATGTAT+AGG + chr1_2:6672911-6672930 None:intergenic 20.0%
!!! AATTGGGTTTTAACATTAAA+AGG - chr1_2:6673348-6673367 Msa0052430:intron 20.0%
!!! ATTGGGTTTTAACATTAAAA+GGG - chr1_2:6673349-6673368 Msa0052430:intron 20.0%
!! CGTATTTAAACTATAGAAAT+GGG + chr1_2:6673432-6673451 None:intergenic 20.0%
!! AATGTTCAAACCTAAAATTT+AGG + chr1_2:6673466-6673485 None:intergenic 20.0%
!!! TTAATGACAATTGATTTGAT+CGG - chr1_2:6673629-6673648 Msa0052430:intron 20.0%
!! AAATTGCTACTCATATATAA+AGG - chr1_2:6673687-6673706 Msa0052430:intron 20.0%
!! AAATAATAAGTTTCCAACAT+TGG + chr1_2:6673800-6673819 None:intergenic 20.0%
!!! TTTTGTCTCTAATTTTTGTT+TGG - chr1_2:6673818-6673837 Msa0052430:intron 20.0%
!! TGAAGAAAATAATAGAACTT+TGG + chr1_2:6673855-6673874 None:intergenic 20.0%
!! ACAAAAAATTGTATGCAAAA+AGG + chr1_2:6673927-6673946 None:intergenic 20.0%
!! GAAATAAAAGAATATTGAAG+AGG + chr1_2:6674053-6674072 None:intergenic 20.0%
!!! ATTCTTTTATTTCTCAGTTT+TGG - chr1_2:6674060-6674079 Msa0052430:CDS 20.0%
!! ATTACCTCATTATTATATGA+AGG + chr1_2:6674369-6674388 None:intergenic 20.0%
!! TTGAGTTCAAAATAGAAATT+GGG + chr1_2:6674600-6674619 None:intergenic 20.0%
!!! TTTGAGTTCAAAATAGAAAT+TGG + chr1_2:6674601-6674620 None:intergenic 20.0%
!! TTAACGATTTAAATAATGAG+AGG + chr1_2:6674627-6674646 None:intergenic 20.0%
!!! CATATTCATTCAAGATTTTT+GGG - chr1_2:6674867-6674886 Msa0052430:five_prime_UTR 20.0%
!! ACAAAATCTTGTATTATATG+AGG - chr1_2:6675112-6675131 Msa0052430:five_prime_UTR 20.0%
! TTTCAAAATGAGATATACGA+TGG + chr1_2:6670914-6670933 None:intergenic 25.0%
! AATGGCACAAAATAGAAAAA+TGG + chr1_2:6671389-6671408 None:intergenic 25.0%
!!! TTCTATTTTGTGCCATTTTT+TGG - chr1_2:6671392-6671411 Msa0052430:intron 25.0%
!!! GCTCTCTAAAATATCAAAAA+TGG + chr1_2:6671535-6671554 None:intergenic 25.0%
! CATGAAAATATTTGCTTTCA+AGG + chr1_2:6671593-6671612 None:intergenic 25.0%
!!! TTAGGTTGTTTCTTCATATA+AGG - chr1_2:6671681-6671700 Msa0052430:intron 25.0%
! AATAATATCGTGTCTATTAG+GGG + chr1_2:6671714-6671733 None:intergenic 25.0%
! CAATAATATCGTGTCTATTA+GGG + chr1_2:6671715-6671734 None:intergenic 25.0%
!!! CTTGAAACTTCTAAAACATA+AGG + chr1_2:6672371-6672390 None:intergenic 25.0%
!!! ATGTTTTAGAAGTTTCAAGT+CGG - chr1_2:6672372-6672391 Msa0052430:intron 25.0%
!!! CTTGAAACTTTTAAAACGTA+AGG + chr1_2:6672400-6672419 None:intergenic 25.0%
!!! ACGTTTTAAAAGTTTCAAGT+TGG - chr1_2:6672401-6672420 Msa0052430:intron 25.0%
!! CAATTATCACCTATTAGTTT+CGG + chr1_2:6672716-6672735 None:intergenic 25.0%
! TAATTGCAATACCCTAATAT+TGG - chr1_2:6672730-6672749 Msa0052430:intron 25.0%
! AAGTAGAAATCATTCTCAAT+TGG - chr1_2:6672924-6672943 Msa0052430:intron 25.0%
! AGTAGAAATCATTCTCAATT+GGG - chr1_2:6672925-6672944 Msa0052430:intron 25.0%
! AAATTTCATGTTGCTCAAAT+TGG - chr1_2:6673331-6673350 Msa0052430:intron 25.0%
! AATTTCATGTTGCTCAAATT+GGG - chr1_2:6673332-6673351 Msa0052430:intron 25.0%
! GCGTATTTAAACTATAGAAA+TGG + chr1_2:6673433-6673452 None:intergenic 25.0%
! TTAATTGCTTTCTAAACTCA+CGG + chr1_2:6673613-6673632 None:intergenic 25.0%
! GAAATGGAAAAAAAAAAGAG+AGG + chr1_2:6673731-6673750 None:intergenic 25.0%
!!! CTCTTTTTTTTTTCCATTTC+AGG - chr1_2:6673731-6673750 Msa0052430:intron 25.0%
!!! TTTTTCGATGGTGAATTTTA+AGG + chr1_2:6673757-6673776 None:intergenic 25.0%
! TGAGTTCAAAATAGAAATTG+GGG + chr1_2:6674599-6674618 None:intergenic 25.0%
! AAATATGATAAATTGCACCT+TGG - chr1_2:6674662-6674681 Msa0052430:five_prime_UTR 25.0%
! TATTTGTTCATCAAATGCAA+TGG - chr1_2:6674735-6674754 Msa0052430:five_prime_UTR 25.0%
! ATTTGTTCATCAAATGCAAT+GGG - chr1_2:6674736-6674755 Msa0052430:five_prime_UTR 25.0%
! ATAAAGATAAATGTGCATCA+AGG + chr1_2:6674795-6674814 None:intergenic 25.0%
! TGCACATTTATCTTTATTGT+AGG - chr1_2:6674798-6674817 Msa0052430:five_prime_UTR 25.0%
!! GCATATTCATTCAAGATTTT+TGG - chr1_2:6674866-6674885 Msa0052430:five_prime_UTR 25.0%
!! TTTTTGACATTATCTTGCTA+AGG + chr1_2:6674898-6674917 None:intergenic 25.0%
! AATCTTGTATTATATGAGGA+AGG - chr1_2:6675116-6675135 Msa0052430:five_prime_UTR 25.0%
!! TCACATATCTCTAGAATTCA+TGG - chr1_2:6671237-6671256 Msa0052430:intron 30.0%
AAAGCTCAGAAAAAATCATG+TGG + chr1_2:6671357-6671376 None:intergenic 30.0%
! TTTTCGTGGTCTTCTAAATT+TGG - chr1_2:6671503-6671522 Msa0052430:CDS 30.0%
TTTCGTGGTCTTCTAAATTT+GGG - chr1_2:6671504-6671523 Msa0052430:CDS 30.0%
AGGCTAGAAAATCCTATAAT+AGG + chr1_2:6671641-6671660 None:intergenic 30.0%
GCAATAATATCGTGTCTATT+AGG + chr1_2:6671716-6671735 None:intergenic 30.0%
! TGAGTTTTACACATCACTAA+TGG - chr1_2:6671825-6671844 Msa0052430:intron 30.0%
TGCCTAATTTACAATTGCTA+TGG - chr1_2:6671919-6671938 Msa0052430:intron 30.0%
CACCATAGCAATTGTAAATT+AGG + chr1_2:6671924-6671943 None:intergenic 30.0%
CTAATTTACAATTGCTATGG+TGG - chr1_2:6671922-6671941 Msa0052430:intron 30.0%
ATGGAAATATTTCACGAATG+AGG + chr1_2:6672213-6672232 None:intergenic 30.0%
TGATCAGAACTTAGAATGAA+AGG + chr1_2:6672282-6672301 None:intergenic 30.0%
! TTTCGGCTTAATTGTAGTTT+TGG + chr1_2:6672699-6672718 None:intergenic 30.0%
ACAATTAAGCCGAAACTAAT+AGG - chr1_2:6672704-6672723 Msa0052430:intron 30.0%
! GGTTAAATATGTTTGAGATC+TGG - chr1_2:6672755-6672774 Msa0052430:intron 30.0%
GAAATTCATAAAGACAAACC+CGG - chr1_2:6672962-6672981 Msa0052430:intron 30.0%
TGTAACGTTCAGTTTATGAT+TGG + chr1_2:6673027-6673046 None:intergenic 30.0%
! TCAAGCTTATGTTTTCTTCA+TGG - chr1_2:6673081-6673100 Msa0052430:intron 30.0%
GATTTATGAATTGACCATGA+AGG - chr1_2:6673151-6673170 Msa0052430:intron 30.0%
! AGTATCAAACTTTTCCTTCA+TGG + chr1_2:6673168-6673187 None:intergenic 30.0%
!!! GTTTGATACTGATTTTTGAC+CGG - chr1_2:6673178-6673197 Msa0052430:intron 30.0%
AAATTCCATGAGTTTGATAC+CGG + chr1_2:6673200-6673219 None:intergenic 30.0%
TTTCATAGTCAAACAAAGCA+AGG - chr1_2:6673496-6673515 Msa0052430:intron 30.0%
TTAGATATTGGTACTAAGCT+CGG + chr1_2:6673538-6673557 None:intergenic 30.0%
! GATCTAAAATGCTTAGACTT+GGG + chr1_2:6673905-6673924 None:intergenic 30.0%
TGGTAGTTTAGTCTCTTAAA+AGG - chr1_2:6673974-6673993 Msa0052430:intron 30.0%
!!! TTCTCAGTTTTGGCTTATTT+AGG - chr1_2:6674070-6674089 Msa0052430:intron 30.0%
TTAGGTGCTTATTGTTTGTA+AGG - chr1_2:6674088-6674107 Msa0052430:intron 30.0%
ATAAGCTTGAACAGTAAAAG+GGG + chr1_2:6674144-6674163 None:intergenic 30.0%
CATAAGCTTGAACAGTAAAA+GGG + chr1_2:6674145-6674164 None:intergenic 30.0%
ACATAAGCTTGAACAGTAAA+AGG + chr1_2:6674146-6674165 None:intergenic 30.0%
TCAGCCTTCATATAATAATG+AGG - chr1_2:6674362-6674381 Msa0052430:intron 30.0%
! TTATGGATTACTCTCTTTGA+TGG - chr1_2:6674919-6674938 Msa0052430:five_prime_UTR 30.0%
! TTATATGAGGAAGGTATATG+TGG - chr1_2:6675125-6675144 Msa0052430:five_prime_UTR 30.0%
! TATATGAGGAAGGTATATGT+GGG - chr1_2:6675126-6675145 Msa0052430:five_prime_UTR 30.0%
TAAAAGTAGATGGAAAGGAG+AGG + chr1_2:6670822-6670841 None:intergenic 35.0%
! AGATCTAAGGTAGAAGATGA+CGG + chr1_2:6670888-6670907 None:intergenic 35.0%
TCATCTTCTACCTTAGATCT+CGG - chr1_2:6670888-6670907 Msa0052430:three_prime_UTR 35.0%
! GATGAAGTGGAGTCTTTTTA+AGG + chr1_2:6670945-6670964 None:intergenic 35.0%
! CGATCATGATTTTCCTTTGA+GGG + chr1_2:6670980-6670999 None:intergenic 35.0%
CCTCAAAGGAAAATCATGAT+CGG - chr1_2:6670978-6670997 Msa0052430:three_prime_UTR 35.0%
! ATCATGATCGGAATTTTTCC+CGG - chr1_2:6670990-6671009 Msa0052430:three_prime_UTR 35.0%
!!! ATAACGCTACTGTGCTTTTT+TGG - chr1_2:6671071-6671090 Msa0052430:CDS 35.0%
!!! TGCTTTTTTGGTTCAGATCT+AGG - chr1_2:6671083-6671102 Msa0052430:CDS 35.0%
!!! TTTTGGTTCAGATCTAGGTT+TGG - chr1_2:6671088-6671107 Msa0052430:CDS 35.0%
TCTAGAGATATGTGAAGTGA+TGG + chr1_2:6671232-6671251 None:intergenic 35.0%
TTTCTTCATATGGTACCTGA+CGG + chr1_2:6671286-6671305 None:intergenic 35.0%
!!! AGAGTCACACAAGTTGATTT+TGG - chr1_2:6671978-6671997 Msa0052430:intron 35.0%
GAAATGCATGAACAACATGA+TGG - chr1_2:6672005-6672024 Msa0052430:intron 35.0%
CTAAAACCCTTCATACTCTT+CGG + chr1_2:6672053-6672072 None:intergenic 35.0%
GAAATATTTCACGAATGAGG+AGG + chr1_2:6672210-6672229 None:intergenic 35.0%
ATGAACCGATAGAAACTGTA+GGG + chr1_2:6672237-6672256 None:intergenic 35.0%
AATGAACCGATAGAAACTGT+AGG + chr1_2:6672238-6672257 None:intergenic 35.0%
! TAAAAACGTGAGCAACGTTT+TGG + chr1_2:6672428-6672447 None:intergenic 35.0%
TGCAATACCCTAATATTGGT+TGG - chr1_2:6672734-6672753 Msa0052430:intron 35.0%
ATATTAAAATGACATCCGCG+AGG - chr1_2:6672814-6672833 Msa0052430:intron 35.0%
AAAAGTTAATAGTCTCCTCG+CGG + chr1_2:6672832-6672851 None:intergenic 35.0%
!! GATTAGTTTGAGAATCAGTC+AGG + chr1_2:6673001-6673020 None:intergenic 35.0%
! GACTTGCATGACATCATTTT+AGG - chr1_2:6673121-6673140 Msa0052430:intron 35.0%
!! TGCATGACATCATTTTAGGT+AGG - chr1_2:6673125-6673144 Msa0052430:intron 35.0%
ATATGACACTTCAAGCAAAG+AGG - chr1_2:6673220-6673239 Msa0052430:intron 35.0%
TAGATATTGGTACTAAGCTC+GGG + chr1_2:6673537-6673556 None:intergenic 35.0%
!!! GTGAATTTTAAGGCCTGAAA+TGG + chr1_2:6673747-6673766 None:intergenic 35.0%
!!! CACGGAAATCATTTTTTCGA+TGG + chr1_2:6673769-6673788 None:intergenic 35.0%
! GGATCTAAAATGCTTAGACT+TGG + chr1_2:6673906-6673925 None:intergenic 35.0%
! ATAATTGGTTCCCTACTTTG+TGG - chr1_2:6673954-6673973 Msa0052430:intron 35.0%
GACTAAACTACCACAAAGTA+GGG + chr1_2:6673967-6673986 None:intergenic 35.0%
AGACTAAACTACCACAAAGT+AGG + chr1_2:6673968-6673987 None:intergenic 35.0%
CTCTTAAAAGGAATGCTTAC+AGG - chr1_2:6673986-6674005 Msa0052430:intron 35.0%
TAAGCTTGAACAGTAAAAGG+GGG + chr1_2:6674143-6674162 None:intergenic 35.0%
AATTCTTGAGCATGACTATG+AGG - chr1_2:6674215-6674234 Msa0052430:intron 35.0%
ATAAATTGCACCTTGGTTGA+TGG - chr1_2:6674669-6674688 Msa0052430:five_prime_UTR 35.0%
GTGTAGAGGAAAGTAAAGAT+TGG + chr1_2:6674763-6674782 None:intergenic 35.0%
TGACTGATGTAATCTTCCTA+TGG - chr1_2:6674826-6674845 Msa0052430:five_prime_UTR 35.0%
TGTAATCTTCCTATGGCATT+TGG - chr1_2:6674833-6674852 Msa0052430:five_prime_UTR 35.0%
! GTTTTGTAGAGGAGATGAAG+TGG + chr1_2:6670958-6670977 None:intergenic 40.0%
!! TTCCTTTGAGGGTTTTGTAG+AGG + chr1_2:6670969-6670988 None:intergenic 40.0%
! CCGATCATGATTTTCCTTTG+AGG + chr1_2:6670981-6671000 None:intergenic 40.0%
! TGTGGTTCTCTTCATTTTCG+TGG - chr1_2:6671489-6671508 Msa0052430:CDS 40.0%
TCGTGTCTATTAGGGGATTA+AGG + chr1_2:6671707-6671726 None:intergenic 40.0%
AAACCACAACAGTGACATGA+AGG + chr1_2:6671953-6671972 None:intergenic 40.0%
! TAAGCTACCGAAGAGTATGA+AGG - chr1_2:6672043-6672062 Msa0052430:intron 40.0%
! AAGCTACCGAAGAGTATGAA+GGG - chr1_2:6672044-6672063 Msa0052430:intron 40.0%
CCATACCCTACAGTTTCTAT+CGG - chr1_2:6672229-6672248 Msa0052430:intron 40.0%
! TCAGGTTAGTTACAAGTTCC+GGG + chr1_2:6672983-6673002 None:intergenic 40.0%
! GTCAGGTTAGTTACAAGTTC+CGG + chr1_2:6672984-6673003 None:intergenic 40.0%
!! TGACATCATTTTAGGTAGGC+AGG - chr1_2:6673129-6673148 Msa0052430:intron 40.0%
TTTGACCGGTATCAAACTCA+TGG - chr1_2:6673192-6673211 Msa0052430:intron 40.0%
CCAACATTGGAGTTTCAACA+CGG + chr1_2:6673787-6673806 None:intergenic 40.0%
! TTCTGCAGGCTACTTTAGTT+GGG - chr1_2:6674711-6674730 Msa0052430:five_prime_UTR 40.0%
GAAGCTAATCCAAATGCCAT+AGG + chr1_2:6674845-6674864 None:intergenic 40.0%
TGGATTACTCTCTTTGATGG+AGG - chr1_2:6674922-6674941 Msa0052430:five_prime_UTR 40.0%
AGGTAGCCATGAACAGTGTA+CGG + chr1_2:6670752-6670771 None:intergenic 45.0%
TATACGATGGCCGAGATCTA+AGG + chr1_2:6670901-6670920 None:intergenic 45.0%
CTCCTCTACAAAACCCTCAA+AGG - chr1_2:6670964-6670983 Msa0052430:three_prime_UTR 45.0%
ACAGTAGCGTTATTCCGTCA+CGG + chr1_2:6671064-6671083 None:intergenic 45.0%
AAGCACTTGAGATGAGAGGT+AGG + chr1_2:6671204-6671223 None:intergenic 45.0%
!! GAAGAAGCACTTGAGATGAG+AGG + chr1_2:6671208-6671227 None:intergenic 45.0%
GGATCTACTGCAAGCAACAA+CGG + chr1_2:6671265-6671284 None:intergenic 45.0%
CTCAAACCTTGAATCATCCC+TGG + chr1_2:6671809-6671828 None:intergenic 45.0%
CAACCTTCATGTCACTGTTG+TGG - chr1_2:6671947-6671966 Msa0052430:intron 45.0%
GACGATTCAGTCTCTTGCAA+AGG - chr1_2:6672077-6672096 Msa0052430:intron 45.0%
CCGATAGAAACTGTAGGGTA+TGG + chr1_2:6672232-6672251 None:intergenic 45.0%
CCGTGTTGAAACTCCAATGT+TGG - chr1_2:6673784-6673803 Msa0052430:intron 45.0%
ATGCTTAGACTTGGGCAAAG+AGG + chr1_2:6673897-6673916 None:intergenic 45.0%
CCAACACTATCCATCAACCA+AGG + chr1_2:6674682-6674701 None:intergenic 45.0%
!! CCTTGGTTGATGGATAGTGT+TGG - chr1_2:6674679-6674698 Msa0052430:five_prime_UTR 45.0%
CAACTAAAGTAGCCTGCAGA+AGG + chr1_2:6674712-6674731 None:intergenic 45.0%
! CTTCTGCAGGCTACTTTAGT+TGG - chr1_2:6674710-6674729 Msa0052430:five_prime_UTR 45.0%
CAAGGACATCAAGTGTGTAG+AGG + chr1_2:6674777-6674796 None:intergenic 45.0%
TAGCCATGAACAGTGTACGG+CGG + chr1_2:6670749-6670768 None:intergenic 50.0%
!! GGTGATGGTGGCATAACCAA+GGG + chr1_2:6670791-6670810 None:intergenic 50.0%
TGGAAAGGAGAGGATGACGA+CGG + chr1_2:6670812-6670831 None:intergenic 50.0%
TGGAAATCACCATCGCCGTA+CGG - chr1_2:6671025-6671044 Msa0052430:exon 50.0%
GGTGGAAGTGATTTCCCGTA+CGG + chr1_2:6671043-6671062 None:intergenic 50.0%
GGAAATCACTTCCACCGTGA+CGG - chr1_2:6671047-6671066 Msa0052430:CDS 50.0%
TTGCTTGCAGTAGATCCGTC+AGG - chr1_2:6671268-6671287 Msa0052430:intron 50.0%
!! TGGTGATGGAGACATGGAGT+AGG + chr1_2:6671785-6671804 None:intergenic 50.0%
AACCTTGAATCATCCCTGGC+TGG + chr1_2:6671805-6671824 None:intergenic 50.0%
CTAAAGTAGCCTGCAGAAGG+TGG + chr1_2:6674709-6674728 None:intergenic 50.0%
! TGAGGAAGGTATATGTGGGC+TGG - chr1_2:6675130-6675149 Msa0052430:five_prime_UTR 50.0%
! GAGGAAGGTATATGTGGGCT+GGG - chr1_2:6675131-6675150 Msa0052430:five_prime_UTR 50.0%
TGAACAGTGTACGGCGGCTA+GGG + chr1_2:6670743-6670762 None:intergenic 55.0%
ATGAACAGTGTACGGCGGCT+AGG + chr1_2:6670744-6670763 None:intergenic 55.0%
TAGCCGCCGTACACTGTTCA+TGG - chr1_2:6670743-6670762 Msa0052430:three_prime_UTR 55.0%
AGGGCGGTTGGAAACGTATG+AGG + chr1_2:6670772-6670791 None:intergenic 55.0%
CATACGTTTCCAACCGCCCT+TGG - chr1_2:6670772-6670791 Msa0052430:three_prime_UTR 55.0%
! GATGGTGGCATAACCAAGGG+CGG + chr1_2:6670788-6670807 None:intergenic 55.0%
!! CGGTGATGGTGGCATAACCA+AGG + chr1_2:6670792-6670811 None:intergenic 55.0%
ATTTCCAGCACGGTGGTCAC+CGG + chr1_2:6671012-6671031 None:intergenic 55.0%
GATGGTGATTTCCAGCACGG+TGG + chr1_2:6671019-6671038 None:intergenic 55.0%
GGCGATGGTGATTTCCAGCA+CGG + chr1_2:6671022-6671041 None:intergenic 55.0%
GGAAATCACCATCGCCGTAC+GGG - chr1_2:6671026-6671045 Msa0052430:exon 55.0%
AGTGATTTCCCGTACGGCGA+TGG + chr1_2:6671037-6671056 None:intergenic 55.0%
GTAGCGTTATTCCGTCACGG+TGG + chr1_2:6671061-6671080 None:intergenic 55.0%
CATGTCTCCATCACCAGCCA+GGG - chr1_2:6671789-6671808 Msa0052430:intron 55.0%
GAATCATCCCTGGCTGGTGA+TGG + chr1_2:6671799-6671818 None:intergenic 55.0%
CACCAGCCAGGGATGATTCA+AGG - chr1_2:6671800-6671819 Msa0052430:intron 55.0%
GTTGGTACTCCACCTTCTGC+AGG - chr1_2:6674697-6674716 Msa0052430:five_prime_UTR 55.0%
!! CGGCTAGGGTTTGTTGCACG+GGG + chr1_2:6670729-6670748 None:intergenic 60.0%
!! GCGGCTAGGGTTTGTTGCAC+GGG + chr1_2:6670730-6670749 None:intergenic 60.0%
!! GGCGGCTAGGGTTTGTTGCA+CGG + chr1_2:6670731-6670750 None:intergenic 60.0%
GTGGCATAACCAAGGGCGGT+TGG + chr1_2:6670784-6670803 None:intergenic 60.0%
! GAGGATGACGACGGTGATGG+TGG + chr1_2:6670803-6670822 None:intergenic 60.0%
GGAGAGGATGACGACGGTGA+TGG + chr1_2:6670806-6670825 None:intergenic 60.0%
TTTCCAGCACGGTGGTCACC+GGG + chr1_2:6671011-6671030 None:intergenic 60.0%
!! CCTGGCTGGTGATGGAGACA+TGG + chr1_2:6671791-6671810 None:intergenic 60.0%
CCATGTCTCCATCACCAGCC+AGG - chr1_2:6671788-6671807 Msa0052430:intron 60.0%
TTTCCCGGTGACCACCGTGC+TGG - chr1_2:6671005-6671024 Msa0052430:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 6670702 6675161 6670702 ID=Msa0052430;Name=Msa0052430
chr1_2 mRNA 6670702 6675161 6670702 ID=Msa0052430-mRNA-1;Parent=Msa0052430;Name=Msa0052430-mRNA-1;_AED=0.37;_eAED=0.37;_QI=554|0.66|0.75|1|0|0.25|4|329|100
chr1_2 exon 6670702 6671144 6670702 ID=Msa0052430-mRNA-1:exon:2201;Parent=Msa0052430-mRNA-1
chr1_2 exon 6671480 6671569 6671480 ID=Msa0052430-mRNA-1:exon:2200;Parent=Msa0052430-mRNA-1
chr1_2 exon 6674017 6674081 6674017 ID=Msa0052430-mRNA-1:exon:2199;Parent=Msa0052430-mRNA-1
chr1_2 exon 6674574 6675161 6674574 ID=Msa0052430-mRNA-1:exon:2198;Parent=Msa0052430-mRNA-1
chr1_2 five_prime_UTR 6674608 6675161 6674608 ID=Msa0052430-mRNA-1:five_prime_utr;Parent=Msa0052430-mRNA-1
chr1_2 CDS 6674574 6674607 6674574 ID=Msa0052430-mRNA-1:cds;Parent=Msa0052430-mRNA-1
chr1_2 CDS 6674017 6674081 6674017 ID=Msa0052430-mRNA-1:cds;Parent=Msa0052430-mRNA-1
chr1_2 CDS 6671480 6671569 6671480 ID=Msa0052430-mRNA-1:cds;Parent=Msa0052430-mRNA-1
chr1_2 CDS 6671031 6671144 6671031 ID=Msa0052430-mRNA-1:cds;Parent=Msa0052430-mRNA-1
chr1_2 three_prime_UTR 6670702 6671030 6670702 ID=Msa0052430-mRNA-1:three_prime_utr;Parent=Msa0052430-mRNA-1
Gene Sequence

>Msa0052430

ATGGATCCGTTGTTGCTTGCAGTAGATCCGTCAGCCTACTCCATGTCTCCATCACCAGCCAGGGATGATTCAAGGTTTGAGTTTTACACATCACTAATGATAAATGATAAAAAGACACAATCTCTTGATTTCACAAGATTTAAGATAGAGCTACTGACAGTTGATGCTCAGCCTTCATATAATAATGAGGCTACTTTAGTTGGGTTATTTGTTCATCAAATGCAATGGGTCCAATCTTTACTTTCCTCTACACACTTGATGTCCTTGATGCACATTTATCTTTATTGTAGGAATGATGACTGA
Protein sequence

>Msa0052430

MDPLLLAVDPSAYSMSPSPARDDSRFEFYTSLMINDKKTQSLDFTRFKIELLTVDAQPSYNNEATLVGLFVHQMQWVQSLLSSTHLMSLMHIYLYCRNDD*