AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0537720

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0537720 MtrunA17_Chr4g0014311 42.262 168 70 2 104 244 118 285 2.50e-33 122
Msa0537720 MtrunA17_Chr8g0365271 46.358 151 70 3 104 243 42 192 7.52e-32 115
Msa0537720 MtrunA17_Chr5g0444831 45.695 151 71 3 104 243 30 180 2.81e-30 111
Msa0537720 MtrunA17_Chr7g0270411 44.961 129 60 2 126 243 1 129 4.89e-25 95.9
Msa0537720 MtrunA17_Chr3g0138341 68.000 50 16 0 104 153 52 101 8.68e-19 80.1
Msa0537720 MtrunA17_Chr2g0300061 39.286 84 51 0 103 186 4 87 1.19e-17 75.5
Msa0537720 MtrunA17_Chr1g0212881 38.462 65 37 1 113 177 35 96 8.11e-13 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 108 sgRNAs with CRISPR-Local

Find 228 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGCCAAAACCATCAAAATT+AGG 0.132100 4_1:+16428598 None:intergenic
TGGGAAACAAATGTTGTTTA+AGG 0.242210 4_1:-16425912 Msa0537720:CDS
TAAACAAAACAACATATAAA+TGG 0.265264 4_1:+16425208 None:intergenic
GAATATTGGTGTGAGTTTAT+TGG 0.271764 4_1:+16428462 None:intergenic
GAAAGGCTTGCTGCAACTTT+AGG 0.274755 4_1:-16427862 Msa0537720:CDS
TTCCCAACCACTTTGCTACT+TGG 0.290059 4_1:+16425458 None:intergenic
TGTGACTGTCCACAAAATTA+AGG 0.297574 4_1:-16425801 Msa0537720:intron
CATCGACATCGGACATATTT+CGG 0.315042 4_1:+16428150 None:intergenic
ATGGGAACTTAAAGCATAAT+TGG 0.316343 4_1:-16425877 Msa0537720:CDS
GACGGATGTGGCGGGGATAT+TGG 0.329689 4_1:+16428283 None:intergenic
TAATCTCCATTCATCTCATT+TGG 0.330111 4_1:-16428053 Msa0537720:intron
AGCCAAAACCATCAAAATTA+GGG 0.360232 4_1:+16428599 None:intergenic
TGGTGTGAGTTTATTGGATT+AGG 0.362585 4_1:+16428468 None:intergenic
ATCAATGCCAATACGGTTGC+CGG 0.366747 4_1:+16428217 None:intergenic
TCAGTTGCGGCTGATGAATC+TGG 0.378282 4_1:+16428181 None:intergenic
GGAGAATTAGCGGCGGCGTT+AGG 0.378302 4_1:+16428238 None:intergenic
TCGGAATGATCCAGAGAGTT+TGG 0.403460 4_1:+16428310 None:intergenic
CGGTAGAGGTGGGAGAATAT+TGG 0.405262 4_1:+16428448 None:intergenic
TCTCCCACCTCTACCGTCTC+TGG 0.417810 4_1:-16428441 Msa0537720:five_prime_UTR
ATCTTCTTCACCAAACTCTC+TGG 0.418334 4_1:-16428320 Msa0537720:five_prime_UTR
TGTTTAATTGTGCAGGGAAC+CGG 0.424669 4_1:-16425971 Msa0537720:intron
GTGTAGATCCGGCTGTTCTC+AGG 0.427894 4_1:+16428122 None:intergenic
GACTTAAAATTGAAAGACAT+TGG 0.431655 4_1:+16428518 None:intergenic
AACGCTAGGTTTGAGGAATT+AGG 0.434175 4_1:-16428082 Msa0537720:CDS
GTTGTTTAAGGTGGAATTCA+CGG 0.447146 4_1:-16425900 Msa0537720:CDS
ATCCAACGGCCATAATAGTT+TGG 0.449119 4_1:+16428576 None:intergenic
GTCAACTGTGTCAATTTCTC+TGG 0.454068 4_1:-16428263 Msa0537720:five_prime_UTR
TTGGGAACAGATGGATTGTA+TGG 0.456955 4_1:+16428537 None:intergenic
ACTTAAAATTGAAAGACATT+GGG 0.460714 4_1:+16428519 None:intergenic
ATACCGGAGGGAGGCAATGA+AGG 0.462165 4_1:+16428411 None:intergenic
GCTGATGAACGCTAGGTTTG+AGG 0.467581 4_1:-16428089 Msa0537720:CDS
CTCCAAACTATTATGGCCGT+TGG 0.468523 4_1:-16428578 Msa0537720:five_prime_UTR
CGAGGTTGCAAGTGTAGATC+CGG 0.469365 4_1:+16428111 None:intergenic
TAATTCTCCGGCAACCGTAT+TGG 0.469409 4_1:-16428224 Msa0537720:five_prime_UTR
TTGCGAGTATCCAATTGATC+TGG 0.475496 4_1:-16425945 Msa0537720:CDS
GCGTTGATCCACATCGTCTA+AGG 0.475507 4_1:-16425288 Msa0537720:three_prime_UTR
TTTAATAATTGCAGTTCCGT+TGG 0.476199 4_1:-16427015 Msa0537720:intron
GAAACAAATGTTGTTTAAGG+TGG 0.479313 4_1:-16425909 Msa0537720:CDS
TGAAAGACATTGGGAACAGA+TGG 0.481807 4_1:+16428528 None:intergenic
TTGACGCAATGAACTTTGAC+AGG 0.484199 4_1:+16425368 None:intergenic
CTCCCTTCATTGCCTCCCTC+CGG 0.488953 4_1:-16428414 Msa0537720:five_prime_UTR
GAGATTGTTGCAGAGAAGAA+AGG 0.502375 4_1:-16427879 Msa0537720:CDS
TTCGGTGGTTACATCAGTTG+CGG 0.506253 4_1:+16428168 None:intergenic
CATTAAAACAATGATACTAC+TGG 0.507765 4_1:+16425324 None:intergenic
GACATACCAAATGAGATGAA+TGG 0.509003 4_1:+16428047 None:intergenic
CACCAAGTAGCAAAGTGGTT+GGG 0.514111 4_1:-16425460 Msa0537720:CDS
AGTTCCAGAGACGGTAGAGG+TGG 0.514295 4_1:+16428437 None:intergenic
ACGACAGATGGAGGCAATGA+CGG 0.515183 4_1:+16428347 None:intergenic
TACGGTTGCCGGAGAATTAG+CGG 0.516224 4_1:+16428228 None:intergenic
ACACAGTTGACGGATGTGGC+GGG 0.519380 4_1:+16428275 None:intergenic
CGGCATGGAAGATGAGATAC+CGG 0.520453 4_1:+16428395 None:intergenic
CGACAGATGGAGGCAATGAC+GGG 0.520995 4_1:+16428348 None:intergenic
CCCTCTTGAACATGCTCCAA+CGG 0.524368 4_1:+16426999 None:intergenic
TAACGCCGCCGCTAATTCTC+CGG 0.529028 4_1:-16428236 Msa0537720:five_prime_UTR
AGAGAAATTGACACAGTTGA+CGG 0.531615 4_1:+16428265 None:intergenic
CAACCTCGCTGATGAACGCT+AGG 0.533506 4_1:-16428096 Msa0537720:CDS
ATTGCCATAACCAACATCTC+AGG 0.534276 4_1:+16425655 None:intergenic
ACTTTCTCTAGATTGGGTGT+TGG 0.538747 4_1:-16427904 Msa0537720:intron
ATGAGAGACCTGAGAACAGC+CGG 0.541466 4_1:-16428130 Msa0537720:CDS
TAGAACACTTGCAGCTAACA+AGG 0.541533 4_1:-16426937 Msa0537720:intron
GTGGTTGGGAAAAGATTAGC+TGG 0.541972 4_1:-16425446 Msa0537720:CDS
ATTGACACAGTTGACGGATG+TGG 0.544827 4_1:+16428271 None:intergenic
TGGCGGGGATATTGGATGAT+CGG 0.555756 4_1:+16428291 None:intergenic
TTTAAGGTGGAATTCACGGA+TGG 0.557502 4_1:-16425896 Msa0537720:CDS
TGTAGAAGCCATTACTTGAG+TGG 0.560318 4_1:-16425527 Msa0537720:intron
TGGAATCACCACTCAAGTAA+TGG 0.564747 4_1:+16425519 None:intergenic
TCATTGCGTCAAAGTTGAGA+AGG 0.566953 4_1:-16425357 Msa0537720:CDS
GATCGCCGCAGCTGACGGCA+TGG 0.567039 4_1:+16428380 None:intergenic
AGCATCAAACGTACATCGAA+AGG 0.568398 4_1:+16425182 None:intergenic
TAACGGATCGCCGCAGCTGA+CGG 0.568917 4_1:+16428375 None:intergenic
TACCGGAGGGAGGCAATGAA+GGG 0.573825 4_1:+16428412 None:intergenic
GAGAGTTCCAGAGACGGTAG+AGG 0.574827 4_1:+16428434 None:intergenic
TCCGTTGGAGCATGTTCAAG+AGG 0.576223 4_1:-16427000 Msa0537720:CDS
AAGAAGATTGAGACGACAGA+TGG 0.579468 4_1:+16428335 None:intergenic
AAAAGATTAGCTGGCAGCGT+GGG 0.580966 4_1:-16425437 Msa0537720:CDS
CACAGTTGACGGATGTGGCG+GGG 0.583831 4_1:+16428276 None:intergenic
ATCTTCCATGCCGTCAGCTG+CGG 0.586670 4_1:-16428385 Msa0537720:five_prime_UTR
ACACCAAGTAGCAAAGTGGT+TGG 0.586766 4_1:-16425461 Msa0537720:CDS
AATTGGATACTCGCAACTAC+CGG 0.587687 4_1:+16425952 None:intergenic
AAAACCATCAAAATTAGGGT+TGG 0.590286 4_1:+16428603 None:intergenic
CTCAGGTCTCTCATCGACAT+CGG 0.590904 4_1:+16428139 None:intergenic
TTAAGGTGGAATTCACGGAT+GGG 0.591193 4_1:-16425895 Msa0537720:CDS
GACACAGTTGACGGATGTGG+CGG 0.593385 4_1:+16428274 None:intergenic
AACGTGGAAGAAACTGAAGT+TGG 0.595322 4_1:-16425410 Msa0537720:CDS
AACTTTAGGAACATTAATTG+CGG 0.604320 4_1:-16427848 Msa0537720:CDS
AAAACAATCCTTAGACGATG+TGG 0.607051 4_1:+16425280 None:intergenic
AAGATTGAGACGACAGATGG+AGG 0.608758 4_1:+16428338 None:intergenic
AGCTTCTCTTGATAGCATAG+TGG 0.608989 4_1:+16425852 None:intergenic
CCGTTGGAGCATGTTCAAGA+GGG 0.612238 4_1:-16426999 Msa0537720:CDS
TTTAGGAACATTAATTGCGG+TGG 0.612421 4_1:-16427845 Msa0537720:intron
TGAAGGGAGAGTTCCAGAGA+CGG 0.614015 4_1:+16428428 None:intergenic
GTATTGGCATTGATGTTGTA+CGG 0.614046 4_1:-16428208 Msa0537720:exon
GTTCCAGAGACGGTAGAGGT+GGG 0.617227 4_1:+16428438 None:intergenic
ATCTATACAGATGCATCCAA+CGG 0.620179 4_1:+16428562 None:intergenic
AGGCAATGACGGGATTGTAA+CGG 0.626022 4_1:+16428358 None:intergenic
TGCTGACAAGATTTGCAACA+AGG 0.629992 4_1:-16425701 Msa0537720:intron
GTACAACATCAATGCCAATA+CGG 0.633208 4_1:+16428210 None:intergenic
AAATACACCAAGTAGCAAAG+TGG 0.642147 4_1:-16425465 Msa0537720:CDS
TGGCAGCGTGGGTTCGAACG+TGG 0.648721 4_1:-16425426 Msa0537720:CDS
ATGGAAGATGAGATACCGGA+GGG 0.655985 4_1:+16428399 None:intergenic
TAAATGGATCAATCCATACA+CGG 0.666552 4_1:+16425224 None:intergenic
GAAGATGAGATACCGGAGGG+AGG 0.668843 4_1:+16428402 None:intergenic
AACTGAGAGCAAATCAAACG+AGG 0.670294 4_1:-16425492 Msa0537720:CDS
GAAAAGATTAGCTGGCAGCG+TGG 0.680628 4_1:-16425438 Msa0537720:CDS
CATGGAAGATGAGATACCGG+AGG 0.681913 4_1:+16428398 None:intergenic
CGACATCGGACATATTTCGG+TGG 0.691640 4_1:+16428153 None:intergenic
AAACCTAGCGTTCATCAGCG+AGG 0.714258 4_1:+16428093 None:intergenic
GGTTGCCGGAGAATTAGCGG+CGG 0.756792 4_1:+16428231 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGAATTATATTACTTTTTTT+CGG - chr4_1:16426150-16426169 Msa0537720:intron 10.0%
!! AATATAATTCATAGATCAAA+AGG + chr4_1:16426141-16426160 None:intergenic 15.0%
!!! TATTTTTCATCTATTGATAA+TGG - chr4_1:16426582-16426601 Msa0537720:intron 15.0%
!! TTTATATAATCATAGATAGA+AGG + chr4_1:16426969-16426988 None:intergenic 15.0%
!!! TCAAAATTATAATAATGGAA+AGG + chr4_1:16427144-16427163 None:intergenic 15.0%
!! AAAAAAAAAAACGTCAAATT+AGG + chr4_1:16427333-16427352 None:intergenic 15.0%
!!! TTGTAAGCTTTAAATAATTA+TGG - chr4_1:16427568-16427587 Msa0537720:intron 15.0%
!!! TGTAAGCTTTAAATAATTAT+GGG - chr4_1:16427569-16427588 Msa0537720:intron 15.0%
!!! GATTTTTTGTTTTTTGTTTT+TGG - chr4_1:16428511-16428530 Msa0537720:five_prime_UTR 15.0%
!! TAAACAAAACAACATATAAA+TGG + chr4_1:16428558-16428577 None:intergenic 15.0%
!! ACTTAAAATTGAAAGACATT+GGG + chr4_1:16425247-16425266 None:intergenic 20.0%
!! AACATGATAAAAGAATAGAA+GGG + chr4_1:16425761-16425780 None:intergenic 20.0%
!! AAACATGATAAAAGAATAGA+AGG + chr4_1:16425762-16425781 None:intergenic 20.0%
!!! TTTATTCCTAATTTTCTAGA+AGG - chr4_1:16425779-16425798 Msa0537720:intron 20.0%
!! TTATTTACTTTCTCTAGATT+GGG - chr4_1:16425853-16425872 Msa0537720:CDS 20.0%
!! ATAGAAATAAACACAAAGAA+TGG + chr4_1:16426093-16426112 None:intergenic 20.0%
!!! AATTTCTTTTGATTAAGTCA+TGG - chr4_1:16426402-16426421 Msa0537720:intron 20.0%
!! TTATATTTGCATCATATCAA+AGG + chr4_1:16426649-16426668 None:intergenic 20.0%
!! TAACTCACTATAATTTACTT+TGG - chr4_1:16426666-16426685 Msa0537720:intron 20.0%
!! ATATTAGAAAGAGTTAAGTT+TGG + chr4_1:16426710-16426729 None:intergenic 20.0%
!! AAAATACACAGACATTTAAT+TGG + chr4_1:16426919-16426938 None:intergenic 20.0%
!!! ATTTGGTTACAATCATTTTA+CGG - chr4_1:16427298-16427317 Msa0537720:intron 20.0%
!!! AGTGATAATATACATGATTT+TGG + chr4_1:16427393-16427412 None:intergenic 20.0%
!!! CATCTATCTTTTGATTTATT+AGG - chr4_1:16427453-16427472 Msa0537720:intron 20.0%
!!! GTTAATGTTACTGATTTTTA+AGG + chr4_1:16427517-16427536 None:intergenic 20.0%
!!! TTGTTTTCTAAATTCAAATG+TGG + chr4_1:16427743-16427762 None:intergenic 20.0%
!! TTGAATTTAGAAAACAAAGA+AGG - chr4_1:16427746-16427765 Msa0537720:intron 20.0%
!!! GTTTGATGCTTTTATATTAA+TGG - chr4_1:16428594-16428613 Msa0537720:five_prime_UTR 20.0%
!! AGAATAAAAAGTAGAAAACA+TGG + chr4_1:16428622-16428641 None:intergenic 20.0%
! GACTTAAAATTGAAAGACAT+TGG + chr4_1:16425248-16425267 None:intergenic 25.0%
!!! ATTTTAAGTCAGAACTACAT+TGG - chr4_1:16425258-16425277 Msa0537720:three_prime_UTR 25.0%
!! AAAACTCCTTCTAGAAAATT+AGG + chr4_1:16425788-16425807 None:intergenic 25.0%
!! TTTTATGCAGTGATGATTTA+TGG - chr4_1:16425805-16425824 Msa0537720:CDS 25.0%
! GTTATTTACTTTCTCTAGAT+TGG - chr4_1:16425852-16425871 Msa0537720:CDS 25.0%
!! AACTTTAGGAACATTAATTG+CGG - chr4_1:16425915-16425934 Msa0537720:CDS 25.0%
!!! TATATTACTTTTTTTCGGCT+AGG - chr4_1:16426155-16426174 Msa0537720:intron 25.0%
! TATAATCATAGATAGAAGGA+TGG + chr4_1:16426965-16426984 None:intergenic 25.0%
! AATCAAAAGATAGATGAGTT+AGG + chr4_1:16427449-16427468 None:intergenic 25.0%
!!! TAATTGTTGTTTAATTGTGC+AGG - chr4_1:16427785-16427804 Msa0537720:intron 25.0%
!!! AATTGTTGTTTAATTGTGCA+GGG - chr4_1:16427786-16427805 Msa0537720:intron 25.0%
!! TAGTTGTACAAGATTTTGTT+CGG - chr4_1:16427985-16428004 Msa0537720:intron 25.0%
!!! CGAGGTTTTAAGTTTTTAAT+TGG - chr4_1:16428188-16428207 Msa0537720:CDS 25.0%
! CATTAAAACAATGATACTAC+TGG + chr4_1:16428442-16428461 None:intergenic 25.0%
AAAACCATCAAAATTAGGGT+TGG + chr4_1:16425163-16425182 None:intergenic 30.0%
!! AACCCTAATTTTGATGGTTT+TGG - chr4_1:16425162-16425181 Msa0537720:three_prime_UTR 30.0%
AGCCAAAACCATCAAAATTA+GGG + chr4_1:16425167-16425186 None:intergenic 30.0%
GAATATTGGTGTGAGTTTAT+TGG + chr4_1:16425304-16425323 None:intergenic 30.0%
TAATCTCCATTCATCTCATT+TGG - chr4_1:16425710-16425729 Msa0537720:intron 30.0%
TGAAGTCTCTCTAAGTAATA+GGG + chr4_1:16426275-16426294 None:intergenic 30.0%
ATGAAGTCTCTCTAAGTAAT+AGG + chr4_1:16426276-16426295 None:intergenic 30.0%
AAAATGGTTTCATCATAACG+TGG + chr4_1:16426308-16426327 None:intergenic 30.0%
!!! AAACCATTTTTAGTTATGCC+AGG - chr4_1:16426318-16426337 Msa0537720:intron 30.0%
AGAAGTTCCTCAATGATTTA+AGG - chr4_1:16426468-16426487 Msa0537720:intron 30.0%
TTTAATAATTGCAGTTCCGT+TGG - chr4_1:16426748-16426767 Msa0537720:intron 30.0%
GCAAAACACTGATATTCAAT+TGG + chr4_1:16426857-16426876 None:intergenic 30.0%
GATTCAATGAAGAACAATCA+GGG + chr4_1:16426885-16426904 None:intergenic 30.0%
TGATTCAATGAAGAACAATC+AGG + chr4_1:16426886-16426905 None:intergenic 30.0%
AAACTGATTGATGAGAAGTA+TGG + chr4_1:16427107-16427126 None:intergenic 30.0%
!!! TTTGTTTGTTTTAGCACCAT+TGG - chr4_1:16427169-16427188 Msa0537720:intron 30.0%
AATCTAATCTAACTCGGATA+CGG + chr4_1:16427204-16427223 None:intergenic 30.0%
ATTCGCAATCTAATCTAACT+CGG + chr4_1:16427210-16427229 None:intergenic 30.0%
! TTGATTTATTAGGTGCTACA+CGG - chr4_1:16427463-16427482 Msa0537720:intron 30.0%
!!! ACACAATCAGGTAGTTTTAT+GGG - chr4_1:16427692-16427711 Msa0537720:intron 30.0%
AACAACAATTATTGCCACTA+TGG + chr4_1:16427776-16427795 None:intergenic 30.0%
ACAAAAACATCCAGATCAAT+TGG + chr4_1:16427831-16427850 None:intergenic 30.0%
!! ATTGATCTGGATGTTTTTGT+TGG - chr4_1:16427831-16427850 Msa0537720:intron 30.0%
!! TTGATCTGGATGTTTTTGTT+GGG - chr4_1:16427832-16427851 Msa0537720:intron 30.0%
! TGGGAAACAAATGTTGTTTA+AGG - chr4_1:16427851-16427870 Msa0537720:CDS 30.0%
! GAAACAAATGTTGTTTAAGG+TGG - chr4_1:16427854-16427873 Msa0537720:CDS 30.0%
ATGGGAACTTAAAGCATAAT+TGG - chr4_1:16427886-16427905 Msa0537720:CDS 30.0%
! GTACAACTACCTTAATTTTG+TGG + chr4_1:16427974-16427993 None:intergenic 30.0%
GAAAACGTAACATATACTTG+TGG + chr4_1:16428166-16428185 None:intergenic 30.0%
! TATATGTTACGTTTTCAACG+AGG - chr4_1:16428170-16428189 Msa0537720:CDS 30.0%
TAAATGGATCAATCCATACA+CGG + chr4_1:16428542-16428561 None:intergenic 30.0%
GAGCCAAAACCATCAAAATT+AGG + chr4_1:16425168-16425187 None:intergenic 35.0%
!!! GTTTTGGCTCCAAACTATTA+TGG - chr4_1:16425178-16425197 Msa0537720:three_prime_UTR 35.0%
ATCTATACAGATGCATCCAA+CGG + chr4_1:16425204-16425223 None:intergenic 35.0%
TGGTGTGAGTTTATTGGATT+AGG + chr4_1:16425298-16425317 None:intergenic 35.0%
AGAGAAATTGACACAGTTGA+CGG + chr4_1:16425501-16425520 None:intergenic 35.0%
GTACAACATCAATGCCAATA+CGG + chr4_1:16425556-16425575 None:intergenic 35.0%
!! GTATTGGCATTGATGTTGTA+CGG - chr4_1:16425555-16425574 Msa0537720:intron 35.0%
GACATACCAAATGAGATGAA+TGG + chr4_1:16425719-16425738 None:intergenic 35.0%
!! TGTGGTTGATGAAACTTATG+TGG - chr4_1:16425829-16425848 Msa0537720:CDS 35.0%
!! GTGGTTGATGAAACTTATGT+GGG - chr4_1:16425830-16425849 Msa0537720:CDS 35.0%
TTTAGGAACATTAATTGCGG+TGG - chr4_1:16425918-16425937 Msa0537720:CDS 35.0%
AATTCACACGTTGAAGATGA+CGG - chr4_1:16426182-16426201 Msa0537720:intron 35.0%
ATAGGGAAAAGTGAAATACG+TGG + chr4_1:16426258-16426277 None:intergenic 35.0%
AGTCCTGGCATAACTAAAAA+TGG + chr4_1:16426324-16426343 None:intergenic 35.0%
CAATGTTCAAATTGAAGTCC+TGG + chr4_1:16426339-16426358 None:intergenic 35.0%
!! CATGGTTGAATTACTGATTC+AGG - chr4_1:16426420-16426439 Msa0537720:intron 35.0%
TCACTTGCCTTAAATCATTG+AGG + chr4_1:16426478-16426497 None:intergenic 35.0%
TTCGCATCTAATCATGTAGA+TGG - chr4_1:16426536-16426555 Msa0537720:intron 35.0%
!! TCATGTAGATGGTTGATAGT+TGG - chr4_1:16426547-16426566 Msa0537720:intron 35.0%
TAGCACAGATGAGAATAGAT+GGG + chr4_1:16427029-16427048 None:intergenic 35.0%
ATAGCACAGATGAGAATAGA+TGG + chr4_1:16427030-16427049 None:intergenic 35.0%
ATCTATTCTCATCTGTGCTA+TGG - chr4_1:16427029-16427048 Msa0537720:intron 35.0%
TATACCTGTCAAACAATGGT+AGG + chr4_1:16427082-16427101 None:intergenic 35.0%
GGTTTATACCTGTCAAACAA+TGG + chr4_1:16427086-16427105 None:intergenic 35.0%
ATCTAATCTAACTCGGATAC+GGG + chr4_1:16427203-16427222 None:intergenic 35.0%
GAACTTGATGTTGAATACGT+AGG - chr4_1:16427263-16427282 Msa0537720:intron 35.0%
! GTAGGTTATTGCTGCTTATT+TGG - chr4_1:16427281-16427300 Msa0537720:intron 35.0%
CAACGTTTGTTTGTATGACA+GGG - chr4_1:16427354-16427373 Msa0537720:intron 35.0%
!! CACACAATCAGGTAGTTTTA+TGG - chr4_1:16427691-16427710 Msa0537720:intron 35.0%
! GTTGTTTAAGGTGGAATTCA+CGG - chr4_1:16427863-16427882 Msa0537720:CDS 35.0%
TGTGACTGTCCACAAAATTA+AGG - chr4_1:16427962-16427981 Msa0537720:intron 35.0%
TTGCATATATCCTGAGATGT+TGG - chr4_1:16428098-16428117 Msa0537720:CDS 35.0%
! ATATCCTGAGATGTTGGTTA+TGG - chr4_1:16428104-16428123 Msa0537720:CDS 35.0%
!!! TGATTTGCTCTCAGTTTTAG+TGG + chr4_1:16428267-16428286 None:intergenic 35.0%
AAATACACCAAGTAGCAAAG+TGG - chr4_1:16428298-16428317 Msa0537720:five_prime_UTR 35.0%
AAAACAATCCTTAGACGATG+TGG + chr4_1:16428486-16428505 None:intergenic 35.0%
! TGTGCCAACCCTAATTTTGA+TGG - chr4_1:16425156-16425175 Msa0537720:three_prime_UTR 40.0%
ATCCAACGGCCATAATAGTT+TGG + chr4_1:16425190-16425209 None:intergenic 40.0%
!! TTGGGAACAGATGGATTGTA+TGG + chr4_1:16425229-16425248 None:intergenic 40.0%
TGAAAGACATTGGGAACAGA+TGG + chr4_1:16425238-16425257 None:intergenic 40.0%
AAGAAGATTGAGACGACAGA+TGG + chr4_1:16425431-16425450 None:intergenic 40.0%
ATCTTCTTCACCAAACTCTC+TGG - chr4_1:16425443-16425462 Msa0537720:CDS 40.0%
GTCAACTGTGTCAATTTCTC+TGG - chr4_1:16425500-16425519 Msa0537720:CDS 40.0%
CATCGACATCGGACATATTT+CGG + chr4_1:16425616-16425635 None:intergenic 40.0%
AACGCTAGGTTTGAGGAATT+AGG - chr4_1:16425681-16425700 Msa0537720:CDS 40.0%
GCAGTGATGATTTATGGTTG+TGG - chr4_1:16425811-16425830 Msa0537720:CDS 40.0%
ACTTTCTCTAGATTGGGTGT+TGG - chr4_1:16425859-16425878 Msa0537720:CDS 40.0%
GAGATTGTTGCAGAGAAGAA+AGG - chr4_1:16425884-16425903 Msa0537720:CDS 40.0%
!!! GCGGTGGTATTTTTTTGTAG+CGG - chr4_1:16425934-16425953 Msa0537720:CDS 40.0%
ATTAGGATTCAAGCCATTGC+AGG + chr4_1:16425995-16426014 None:intergenic 40.0%
GAGAGCGAGAAACAAACATT+AGG + chr4_1:16426012-16426031 None:intergenic 40.0%
GAAAAGTGAAATACGTGGAG+GGG + chr4_1:16426253-16426272 None:intergenic 40.0%
GGAAAAGTGAAATACGTGGA+GGG + chr4_1:16426254-16426273 None:intergenic 40.0%
GGCAAGTGAAGAAGAAATGA+TGG - chr4_1:16426489-16426508 Msa0537720:intron 40.0%
TAGAAGCTTATGCACGAAGA+AGG - chr4_1:16426613-16426632 Msa0537720:intron 40.0%
TAGAACACTTGCAGCTAACA+AGG - chr4_1:16426826-16426845 Msa0537720:intron 40.0%
! TCTGTGTATTTTCCCATGCA+AGG - chr4_1:16426927-16426946 Msa0537720:intron 40.0%
AATGGTAGGACATCAATGCT+TGG + chr4_1:16427068-16427087 None:intergenic 40.0%
ATGTCCTACCATTGTTTGAC+AGG - chr4_1:16427075-16427094 Msa0537720:intron 40.0%
TCTAATCTAACTCGGATACG+GGG + chr4_1:16427202-16427221 None:intergenic 40.0%
CATCAAGTACAGGACATATG+TGG - chr4_1:16427238-16427257 Msa0537720:intron 40.0%
GCAACGTTTGTTTGTATGAC+AGG - chr4_1:16427353-16427372 Msa0537720:intron 40.0%
TACACTTGAGTCACACAATC+AGG - chr4_1:16427680-16427699 Msa0537720:intron 40.0%
TGTTTAATTGTGCAGGGAAC+CGG - chr4_1:16427792-16427811 Msa0537720:intron 40.0%
AATTGGATACTCGCAACTAC+CGG + chr4_1:16427814-16427833 None:intergenic 40.0%
TTGCGAGTATCCAATTGATC+TGG - chr4_1:16427818-16427837 Msa0537720:intron 40.0%
! TTTAAGGTGGAATTCACGGA+TGG - chr4_1:16427867-16427886 Msa0537720:CDS 40.0%
TTAAGGTGGAATTCACGGAT+GGG - chr4_1:16427868-16427887 Msa0537720:CDS 40.0%
! AGCTTCTCTTGATAGCATAG+TGG + chr4_1:16427914-16427933 None:intergenic 40.0%
TGCTGACAAGATTTGCAACA+AGG - chr4_1:16428062-16428081 Msa0537720:CDS 40.0%
ATTGCCATAACCAACATCTC+AGG + chr4_1:16428111-16428130 None:intergenic 40.0%
CGATGACAACATACATCAAG+TGG + chr4_1:16428141-16428160 None:intergenic 40.0%
TGTAGAAGCCATTACTTGAG+TGG - chr4_1:16428236-16428255 Msa0537720:five_prime_UTR 40.0%
TGGAATCACCACTCAAGTAA+TGG + chr4_1:16428247-16428266 None:intergenic 40.0%
AACTGAGAGCAAATCAAACG+AGG - chr4_1:16428271-16428290 Msa0537720:five_prime_UTR 40.0%
AACGTGGAAGAAACTGAAGT+TGG - chr4_1:16428353-16428372 Msa0537720:five_prime_UTR 40.0%
TTGACGCAATGAACTTTGAC+AGG + chr4_1:16428398-16428417 None:intergenic 40.0%
TCATTGCGTCAAAGTTGAGA+AGG - chr4_1:16428406-16428425 Msa0537720:five_prime_UTR 40.0%
!!! GTTTTTGGTGTTTCCGTGTA+TGG - chr4_1:16428526-16428545 Msa0537720:five_prime_UTR 40.0%
AGCATCAAACGTACATCGAA+AGG + chr4_1:16428584-16428603 None:intergenic 40.0%
CTCCAAACTATTATGGCCGT+TGG - chr4_1:16425185-16425204 Msa0537720:three_prime_UTR 45.0%
ATGGAAGATGAGATACCGGA+GGG + chr4_1:16425367-16425386 None:intergenic 45.0%
! AGGCAATGACGGGATTGTAA+CGG + chr4_1:16425408-16425427 None:intergenic 45.0%
AAGATTGAGACGACAGATGG+AGG + chr4_1:16425428-16425447 None:intergenic 45.0%
TCGGAATGATCCAGAGAGTT+TGG + chr4_1:16425456-16425475 None:intergenic 45.0%
! ATTGACACAGTTGACGGATG+TGG + chr4_1:16425495-16425514 None:intergenic 45.0%
TAATTCTCCGGCAACCGTAT+TGG - chr4_1:16425539-16425558 Msa0537720:intron 45.0%
ATCAATGCCAATACGGTTGC+CGG + chr4_1:16425549-16425568 None:intergenic 45.0%
TTCGGTGGTTACATCAGTTG+CGG + chr4_1:16425598-16425617 None:intergenic 45.0%
!! GAAAGGCTTGCTGCAACTTT+AGG - chr4_1:16425901-16425920 Msa0537720:CDS 45.0%
TGGTGATTTCCTTCCTGCAA+TGG - chr4_1:16425979-16425998 Msa0537720:intron 45.0%
GGGAAAAGTGAAATACGTGG+AGG + chr4_1:16426255-16426274 None:intergenic 45.0%
! TGCACGAAGAAGGTTTTCGA+AGG - chr4_1:16426623-16426642 Msa0537720:intron 45.0%
! ATTTTCCCATGCAAGGCTGT+AGG - chr4_1:16426934-16426953 Msa0537720:intron 45.0%
TCATACCTACAGCCTTGCAT+GGG + chr4_1:16426942-16426961 None:intergenic 45.0%
!! ATAGATGGGCTACACATGCA+TGG + chr4_1:16427015-16427034 None:intergenic 45.0%
ATGAGTTGCGCATCAAGTAC+AGG - chr4_1:16427228-16427247 Msa0537720:intron 45.0%
CAAGTACAGGACATATGTGG+TGG - chr4_1:16427241-16427260 Msa0537720:intron 45.0%
!! ACAAAGAAGGCGTTCCATAG+TGG - chr4_1:16427759-16427778 Msa0537720:intron 45.0%
ACACCAAGTAGCAAAGTGGT+TGG - chr4_1:16428302-16428321 Msa0537720:five_prime_UTR 45.0%
CACCAAGTAGCAAAGTGGTT+GGG - chr4_1:16428303-16428322 Msa0537720:five_prime_UTR 45.0%
TTCCCAACCACTTTGCTACT+TGG + chr4_1:16428308-16428327 None:intergenic 45.0%
GTGGTTGGGAAAAGATTAGC+TGG - chr4_1:16428317-16428336 Msa0537720:five_prime_UTR 45.0%
! AAAAGATTAGCTGGCAGCGT+GGG - chr4_1:16428326-16428345 Msa0537720:five_prime_UTR 45.0%
!! AAAAATCAAAATTATAATAA+TGG + chr4_1:16427149-16427168 None:intergenic 5.0%
CGGTAGAGGTGGGAGAATAT+TGG + chr4_1:16425318-16425337 None:intergenic 50.0%
TGAAGGGAGAGTTCCAGAGA+CGG + chr4_1:16425338-16425357 None:intergenic 50.0%
CATGGAAGATGAGATACCGG+AGG + chr4_1:16425368-16425387 None:intergenic 50.0%
CGGCATGGAAGATGAGATAC+CGG + chr4_1:16425371-16425390 None:intergenic 50.0%
ACGACAGATGGAGGCAATGA+CGG + chr4_1:16425419-16425438 None:intergenic 50.0%
TGGCGGGGATATTGGATGAT+CGG + chr4_1:16425475-16425494 None:intergenic 50.0%
TACGGTTGCCGGAGAATTAG+CGG + chr4_1:16425538-16425557 None:intergenic 50.0%
TCAGTTGCGGCTGATGAATC+TGG + chr4_1:16425585-16425604 None:intergenic 50.0%
CGACATCGGACATATTTCGG+TGG + chr4_1:16425613-16425632 None:intergenic 50.0%
CTCAGGTCTCTCATCGACAT+CGG + chr4_1:16425627-16425646 None:intergenic 50.0%
ATGAGAGACCTGAGAACAGC+CGG - chr4_1:16425633-16425652 Msa0537720:intron 50.0%
CGAGGTTGCAAGTGTAGATC+CGG + chr4_1:16425655-16425674 None:intergenic 50.0%
AAACCTAGCGTTCATCAGCG+AGG + chr4_1:16425673-16425692 None:intergenic 50.0%
GCTGATGAACGCTAGGTTTG+AGG - chr4_1:16425674-16425693 Msa0537720:CDS 50.0%
!!! GCTCACGCTGATGGTTTTGA+TGG - chr4_1:16425956-16425975 Msa0537720:CDS 50.0%
!!! CACGCTGATGGTTTTGATGG+TGG - chr4_1:16425959-16425978 Msa0537720:CDS 50.0%
GGATTCAAGCCATTGCAGGA+AGG + chr4_1:16425991-16426010 None:intergenic 50.0%
TCCGTTGGAGCATGTTCAAG+AGG - chr4_1:16426763-16426782 Msa0537720:intron 50.0%
CCCTCTTGAACATGCTCCAA+CGG + chr4_1:16426767-16426786 None:intergenic 50.0%
CCGTTGGAGCATGTTCAAGA+GGG - chr4_1:16426764-16426783 Msa0537720:intron 50.0%
GTCATACCTACAGCCTTGCA+TGG + chr4_1:16426943-16426962 None:intergenic 50.0%
TCTAACTCGGATACGGGGTT+CGG + chr4_1:16427197-16427216 None:intergenic 50.0%
CGATGAATATCAGACGCGCT+AGG - chr4_1:16427610-16427629 Msa0537720:intron 50.0%
! GAAAAGATTAGCTGGCAGCG+TGG - chr4_1:16428325-16428344 Msa0537720:five_prime_UTR 50.0%
! GCGTTGATCCACATCGTCTA+AGG - chr4_1:16428475-16428494 Msa0537720:five_prime_UTR 50.0%
GTTCCAGAGACGGTAGAGGT+GGG + chr4_1:16425328-16425347 None:intergenic 55.0%
AGTTCCAGAGACGGTAGAGG+TGG + chr4_1:16425329-16425348 None:intergenic 55.0%
GAGAGTTCCAGAGACGGTAG+AGG + chr4_1:16425332-16425351 None:intergenic 55.0%
TACCGGAGGGAGGCAATGAA+GGG + chr4_1:16425354-16425373 None:intergenic 55.0%
ATACCGGAGGGAGGCAATGA+AGG + chr4_1:16425355-16425374 None:intergenic 55.0%
GAAGATGAGATACCGGAGGG+AGG + chr4_1:16425364-16425383 None:intergenic 55.0%
ATCTTCCATGCCGTCAGCTG+CGG - chr4_1:16425378-16425397 Msa0537720:CDS 55.0%
CGACAGATGGAGGCAATGAC+GGG + chr4_1:16425418-16425437 None:intergenic 55.0%
! ACACAGTTGACGGATGTGGC+GGG + chr4_1:16425491-16425510 None:intergenic 55.0%
! GACACAGTTGACGGATGTGG+CGG + chr4_1:16425492-16425511 None:intergenic 55.0%
TAACGCCGCCGCTAATTCTC+CGG - chr4_1:16425527-16425546 Msa0537720:intron 55.0%
GTGTAGATCCGGCTGTTCTC+AGG + chr4_1:16425644-16425663 None:intergenic 55.0%
CAACCTCGCTGATGAACGCT+AGG - chr4_1:16425667-16425686 Msa0537720:intron 55.0%
TTTGTAGCGGCTCACGCTGA+TGG - chr4_1:16425947-16425966 Msa0537720:CDS 55.0%
GATACGGGGTTCGGATCCAA+TGG + chr4_1:16427188-16427207 None:intergenic 55.0%
TCTCCCACCTCTACCGTCTC+TGG - chr4_1:16425322-16425341 Msa0537720:three_prime_UTR 60.0%
CTCCCTTCATTGCCTCCCTC+CGG - chr4_1:16425349-16425368 Msa0537720:exon 60.0%
! TAACGGATCGCCGCAGCTGA+CGG + chr4_1:16425391-16425410 None:intergenic 60.0%
! GACGGATGTGGCGGGGATAT+TGG + chr4_1:16425483-16425502 None:intergenic 60.0%
! CACAGTTGACGGATGTGGCG+GGG + chr4_1:16425490-16425509 None:intergenic 60.0%
GGAGAATTAGCGGCGGCGTT+AGG + chr4_1:16425528-16425547 None:intergenic 60.0%
GGTTGCCGGAGAATTAGCGG+CGG + chr4_1:16425535-16425554 None:intergenic 60.0%
TGGCAGCGTGGGTTCGAACG+TGG - chr4_1:16428337-16428356 Msa0537720:five_prime_UTR 65.0%
GATCGCCGCAGCTGACGGCA+TGG + chr4_1:16425386-16425405 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 16425139 16428646 16425139 ID=Msa0537720;Name=Msa0537720
chr4_1 mRNA 16425139 16428646 16425139 ID=Msa0537720-mRNA-1;Parent=Msa0537720;Name=Msa0537720-mRNA-1;_AED=0.25;_eAED=0.25;_QI=428|0.4|0.66|1|1|1|6|213|244
chr4_1 exon 16425139 16425544 16425139 ID=Msa0537720-mRNA-1:exon:4948;Parent=Msa0537720-mRNA-1
chr4_1 exon 16425670 16425713 16425670 ID=Msa0537720-mRNA-1:exon:4947;Parent=Msa0537720-mRNA-1
chr4_1 exon 16425802 16425978 16425802 ID=Msa0537720-mRNA-1:exon:4946;Parent=Msa0537720-mRNA-1
chr4_1 exon 16426938 16427023 16426938 ID=Msa0537720-mRNA-1:exon:4945;Parent=Msa0537720-mRNA-1
chr4_1 exon 16427846 16427915 16427846 ID=Msa0537720-mRNA-1:exon:4944;Parent=Msa0537720-mRNA-1
chr4_1 exon 16428054 16428646 16428054 ID=Msa0537720-mRNA-1:exon:4943;Parent=Msa0537720-mRNA-1
chr4_1 five_prime_UTR 16428219 16428646 16428219 ID=Msa0537720-mRNA-1:five_prime_utr;Parent=Msa0537720-mRNA-1
chr4_1 CDS 16428054 16428218 16428054 ID=Msa0537720-mRNA-1:cds;Parent=Msa0537720-mRNA-1
chr4_1 CDS 16427846 16427915 16427846 ID=Msa0537720-mRNA-1:cds;Parent=Msa0537720-mRNA-1
chr4_1 CDS 16426938 16427023 16426938 ID=Msa0537720-mRNA-1:cds;Parent=Msa0537720-mRNA-1
chr4_1 CDS 16425802 16425978 16425802 ID=Msa0537720-mRNA-1:cds;Parent=Msa0537720-mRNA-1
chr4_1 CDS 16425670 16425713 16425670 ID=Msa0537720-mRNA-1:cds;Parent=Msa0537720-mRNA-1
chr4_1 CDS 16425352 16425544 16425352 ID=Msa0537720-mRNA-1:cds;Parent=Msa0537720-mRNA-1
chr4_1 three_prime_UTR 16425139 16425351 16425139 ID=Msa0537720-mRNA-1:three_prime_utr;Parent=Msa0537720-mRNA-1
Gene Sequence

>Msa0537720

ATGTTGTACGGCTGTCCAGATTCATCAGCCGCAACTGATGTAACCACCGAAATATGTCCGATGTCGATGAGAGACCTGAGAACAGCCGGATCTACACTTGCAACCTCGCTGATGAACGCTAGGTTTGAGGAATTAGGTCTCTTTAATCTCCATTCATCTCATTTGATTGGGTGTTGGTCGAGATTGTTGCAGAGAAGAAAGGCTTGCTGCAACTTTAGGAACATTAATTGCGGTGTTCCGTTGGAGCATGTTCAAGAGGGAACTAATGATGCTTATCTTTATGATATGAAGATCAAATTTAGAACACTTGCAGCTAACAAGGGAACCGGTAGTTGCGAGTATCCAATTGATCTGGATGTTTTTGTTGGGAAACAAATGTTGTTTAAGGTGGAATTCACGGATGGGAACTTAAAGCATAATTGGCGCCACTATGCTATCAAGAGAAGCTCTGATGACGCGAATCTTATTACTCAATTTGTGACTGTCCACAAAATTAAGATTTGCAACAAGGACAATGAAGCTGATTGCATATATCCTGAGATAAGCCATTACTTGAGTGGTGATTCCACTAAAACTGAGAGCAAATCAAACGAGGATGAAAATACACCAAGTAGCAAAGTGGTTGGGAAAAGATTAGCTGGCAGCGTGGGTTCGAACGTGGAAGAAACTGAAGTTGGTGAAAGATCGATCAACAAACCTGTCAAAGTTCATTGCGTCAAAGTTGAGAAGGATTAG
Protein sequence

>Msa0537720

MLYGCPDSSAATDVTTEICPMSMRDLRTAGSTLATSLMNARFEELGLFNLHSSHLIGCWSRLLQRRKACCNFRNINCGVPLEHVQEGTNDAYLYDMKIKFRTLAANKGTGSCEYPIDLDVFVGKQMLFKVEFTDGNLKHNWRHYAIKRSSDDANLITQFVTVHKIKICNKDNEADCIYPEISHYLSGDSTKTESKSNEDENTPSSKVVGKRLAGSVGSNVEETEVGERSINKPVKVHCVKVEKD*