Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0053620 | AES59206.1 | 81.034 | 58 | 11 | 0 | 3 | 60 | 4 | 61 | 2.10e-24 | 96.3 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0053620 | G7I9U5 | 81.034 | 58 | 11 | 0 | 3 | 60 | 4 | 61 | 1.00e-24 | 96.3 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0053620 | Msa1390700 | 0.801897 | 7.249257e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 7 sgRNAs with CRISPR-Local
Find 12 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGTGCGTTGGTGTATTT+TGG | 0.279813 | 1_2:+8097459 | None:intergenic |
| GCTGCAGTGTTGTGGCGTTT+TGG | 0.319085 | 1_2:+8097562 | None:intergenic |
| TGACGTTGTATCTTGCTTGC+TGG | 0.391223 | 1_2:+8097593 | None:intergenic |
| CTAGAACATCAGAGCAGGAC+TGG | 0.484139 | 1_2:-8097535 | Msa0053620:CDS |
| AGCAGGACTGGAACAGCTGA+AGG | 0.570373 | 1_2:-8097523 | Msa0053620:CDS |
| CAGAACTAGAACATCAGAGC+AGG | 0.604354 | 1_2:-8097540 | Msa0053620:CDS |
| CTAGTTCTGCTGCAGTGTTG+TGG | 0.612479 | 1_2:+8097554 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATTTTGGTTGAAGTCAAAG+GGG | + | chr1_2:8097607-8097626 | None:intergenic | 30.0% |
| !!! | GTATTTTGGTTGAAGTCAAA+GGG | + | chr1_2:8097608-8097627 | None:intergenic | 30.0% |
| !!! | TGTATTTTGGTTGAAGTCAA+AGG | + | chr1_2:8097609-8097628 | None:intergenic | 30.0% |
| !! | AAAAGTGCGTTGGTGTATTT+TGG | + | chr1_2:8097622-8097641 | None:intergenic | 35.0% |
| TGACGTTGTATCTTGCTTGC+TGG | + | chr1_2:8097488-8097507 | None:intergenic | 45.0% | |
| CAGAACTAGAACATCAGAGC+AGG | - | chr1_2:8097538-8097557 | Msa0053620:CDS | 45.0% | |
| ! | TGCAGTGTTGTGGCGTTTTG+GGG | + | chr1_2:8097517-8097536 | None:intergenic | 50.0% |
| ! | CTGCAGTGTTGTGGCGTTTT+GGG | + | chr1_2:8097518-8097537 | None:intergenic | 50.0% |
| !! | CTAGTTCTGCTGCAGTGTTG+TGG | + | chr1_2:8097527-8097546 | None:intergenic | 50.0% |
| CTAGAACATCAGAGCAGGAC+TGG | - | chr1_2:8097543-8097562 | Msa0053620:CDS | 50.0% | |
| ! | GCTGCAGTGTTGTGGCGTTT+TGG | + | chr1_2:8097519-8097538 | None:intergenic | 55.0% |
| AGCAGGACTGGAACAGCTGA+AGG | - | chr1_2:8097555-8097574 | Msa0053620:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_2 | gene | 8097450 | 8097650 | 8097450 | ID=Msa0053620;Name=Msa0053620 |
| chr1_2 | mRNA | 8097450 | 8097650 | 8097450 | ID=Msa0053620-mRNA-1;Parent=Msa0053620;Name=Msa0053620-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0|-1|0|1|-1|1|1|0|66 |
| chr1_2 | exon | 8097450 | 8097650 | 8097450 | ID=Msa0053620-mRNA-1:exon:2870;Parent=Msa0053620-mRNA-1 |
| chr1_2 | CDS | 8097450 | 8097650 | 8097450 | ID=Msa0053620-mRNA-1:cds;Parent=Msa0053620-mRNA-1 |
| Gene Sequence |
| Protein sequence |
