AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1390700

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1390700 MtrunA17_Chr2g0325361 61.378 624 192 5 5 594 469 1077 0.0 620
Msa1390700 MtrunA17_Chr2g0325361 45.961 359 169 2 1 334 349 707 1.14e-65 232
Msa1390700 MtrunA17_Chr2g0325361 39.706 408 188 6 2 356 252 654 1.44e-49 185
Msa1390700 MtrunA17_Chr2g0325361 42.966 263 115 1 2 229 550 812 4.76e-40 157
Msa1390700 MtrunA17_Chr2g0325361 32.627 236 120 4 111 325 239 456 1.07e-15 80.9
Msa1390700 MtrunA17_Chr2g0325661 56.129 620 231 5 9 594 264 876 0.0 562
Msa1390700 MtrunA17_Chr2g0325661 45.249 221 86 3 2 208 397 596 4.67e-27 116
Msa1390700 MtrunA17_Chr2g0325661 35.821 201 107 4 169 355 224 416 6.65e-13 72.0
Msa1390700 MtrunA17_Chr6g0471251 50.891 617 255 8 15 594 162 767 2.75e-165 490
Msa1390700 MtrunA17_Chr6g0471251 40.909 220 102 2 2 207 281 486 1.62e-22 102
Msa1390700 MtrunA17_Chr2g0325641 81.928 332 25 3 14 331 1 311 2.14e-164 471
Msa1390700 MtrunA17_Chr2g0325641 41.063 207 80 3 1 165 100 306 1.76e-26 110
Msa1390700 MtrunA17_Chr2g0325641 39.869 153 71 2 1 153 177 308 1.29e-14 75.5
Msa1390700 MtrunA17_Chr2g0325611 56.746 504 176 6 127 595 95 591 1.22e-163 479
Msa1390700 MtrunA17_Chr2g0325611 37.745 204 104 4 44 235 117 309 1.42e-21 99.0
Msa1390700 MtrunA17_Chr2g0325611 41.579 190 88 3 19 208 144 310 2.23e-21 98.2
Msa1390700 MtrunA17_Chr2g0325611 38.333 180 102 2 1 180 140 310 1.03e-16 83.6
Msa1390700 MtrunA17_Chr2g0325611 40.496 121 72 0 8 128 208 328 1.18e-13 73.9
Msa1390700 MtrunA17_Chr2g0325611 35.119 168 74 3 2 162 181 320 9.77e-11 64.7
Msa1390700 MtrunA17_Chr2g0325351 54.713 541 219 8 77 595 153 689 2.26e-158 469
Msa1390700 MtrunA17_Chr2g0325351 42.211 199 91 4 4 202 229 403 7.34e-20 94.0
Msa1390700 MtrunA17_Chr2g0325351 39.153 189 90 2 2 179 241 415 1.54e-16 83.2
Msa1390700 MtrunA17_Chr2g0325631 90.566 265 19 1 336 594 1 265 3.08e-155 446
Msa1390700 MtrunA17_Chr2g0325231 52.197 569 186 7 2 504 334 882 1.43e-154 466
Msa1390700 MtrunA17_Chr2g0325231 42.136 337 155 5 43 340 204 539 2.04e-42 163
Msa1390700 MtrunA17_Chr2g0325231 41.743 218 117 1 1 208 484 701 4.12e-30 126
Msa1390700 MtrunA17_Chr2g0325231 31.935 310 142 7 87 327 140 449 7.27e-17 84.7
Msa1390700 MtrunA17_Chr2g0325621 52.941 544 200 8 77 594 147 660 8.82e-148 441
Msa1390700 MtrunA17_Chr2g0325621 33.951 162 91 2 3 150 266 425 1.57e-11 67.4
Msa1390700 MtrunA17_Chr2g0325321 58.921 482 163 3 14 495 310 756 9.73e-144 434
Msa1390700 MtrunA17_Chr2g0325321 41.781 292 142 2 2 265 312 603 1.97e-41 160
Msa1390700 MtrunA17_Chr2g0325321 46.725 229 122 0 1 229 367 595 2.86e-40 156
Msa1390700 MtrunA17_Chr2g0325321 33.080 263 144 4 86 334 197 441 7.86e-14 74.7
Msa1390700 MtrunA17_Chr2g0325811 49.149 529 247 4 82 593 307 830 5.85e-139 424
Msa1390700 MtrunA17_Chr2g0325811 33.745 243 123 3 13 217 336 578 1.20e-21 99.8
Msa1390700 MtrunA17_Chr2g0325711 46.630 549 245 4 75 575 270 818 6.67e-138 421
Msa1390700 MtrunA17_Chr2g0325711 36.232 207 128 1 2 208 351 553 5.95e-25 110
Msa1390700 MtrunA17_Chr2g0325711 30.108 279 143 4 101 334 167 438 6.53e-16 81.6
Msa1390700 MtrunA17_Chr5g0434761 75.564 266 59 1 336 595 1 266 6.27e-137 399
Msa1390700 MtrunA17_Chr2g0280141 46.886 578 286 8 27 594 251 817 1.53e-134 412
Msa1390700 MtrunA17_Chr2g0325861 45.102 541 258 6 2 513 311 841 2.58e-112 355
Msa1390700 MtrunA17_Chr2g0325861 39.329 328 174 3 16 336 259 568 1.85e-36 145
Msa1390700 MtrunA17_Chr2g0325861 36.704 267 152 2 82 338 269 528 3.73e-23 104
Msa1390700 MtrunA17_Chr5g0434751 67.029 276 63 2 84 331 1 276 9.21e-97 297
Msa1390700 MtrunA17_Chr5g0434751 41.985 262 117 4 2 228 3 264 2.21e-29 118
Msa1390700 MtrunA17_Chr2g0325701 47.241 453 201 9 69 495 147 587 1.56e-96 307
Msa1390700 MtrunA17_Chr2g0325701 36.863 255 132 5 8 234 158 411 2.82e-24 107
Msa1390700 MtrunA17_Chr2g0325701 38.272 162 86 1 2 149 249 410 1.99e-15 79.7
Msa1390700 MtrunA17_Chr5g0416271 48.295 352 132 4 166 495 157 480 1.57e-84 272
Msa1390700 MtrunA17_Chr5g0416271 41.919 198 71 4 13 181 145 327 4.66e-23 102
Msa1390700 MtrunA17_Chr2g0325691 47.692 390 137 6 2 325 322 710 9.34e-75 253
Msa1390700 MtrunA17_Chr2g0325691 43.085 376 147 6 2 325 294 654 1.31e-52 192
Msa1390700 MtrunA17_Chr2g0325691 38.835 412 167 7 1 328 219 629 2.61e-47 177
Msa1390700 MtrunA17_Chr2g0325691 46.416 293 141 5 39 328 207 486 1.58e-41 160
Msa1390700 MtrunA17_Chr2g0325691 37.681 345 154 10 8 325 146 456 2.06e-28 120
Msa1390700 MtrunA17_Chr2g0325691 42.925 212 93 1 2 213 534 717 5.70e-26 113
Msa1390700 MtrunA17_Chr2g0333341 42.786 402 175 7 136 537 276 622 3.52e-72 243
Msa1390700 MtrunA17_Chr2g0333341 43.429 175 78 5 15 188 295 449 1.67e-18 89.4
Msa1390700 MtrunA17_Chr2g0325331 72.903 155 42 0 336 490 1 155 6.10e-66 213
Msa1390700 MtrunA17_Chr2g0325281 49.235 327 131 3 5 331 301 592 5.72e-65 224
Msa1390700 MtrunA17_Chr2g0325281 40.728 302 116 3 82 334 252 539 2.58e-42 161
Msa1390700 MtrunA17_Chr2g0325281 33.460 263 143 4 86 334 197 441 8.46e-21 96.7
Msa1390700 MtrunA17_Chr2g0325281 42.222 135 64 1 2 136 466 586 5.48e-15 78.2
Msa1390700 MtrunA17_Chr2g0332961 44.000 375 145 9 103 470 37 353 8.35e-64 213
Msa1390700 MtrunA17_Chr2g0325761 47.041 338 151 4 9 325 259 589 2.50e-62 218
Msa1390700 MtrunA17_Chr2g0325761 41.304 276 113 3 2 235 322 590 2.09e-36 144
Msa1390700 MtrunA17_Chr2g0325761 45.815 227 88 3 2 214 392 597 1.63e-33 135
Msa1390700 MtrunA17_Chr2g0325761 59.000 100 39 2 491 590 592 689 1.85e-25 111
Msa1390700 MtrunA17_Chr2g0325761 30.744 309 133 4 107 334 220 528 4.83e-19 91.3
Msa1390700 MtrunA17_Chr2g0325761 32.768 177 91 1 3 179 456 604 4.47e-12 68.9
Msa1390700 MtrunA17_Chr2g0325291 57.143 203 81 1 353 549 4 206 7.68e-61 201
Msa1390700 MtrunA17_Chr2g0332951 48.980 245 80 7 255 495 2 205 1.92e-54 184
Msa1390700 MtrunA17_Chr2g0325771 65.680 169 48 2 329 495 10 170 1.31e-51 175
Msa1390700 MtrunA17_Chr2g0325241 40.122 329 164 3 92 417 121 419 2.87e-51 182
Msa1390700 MtrunA17_Chr2g0325241 37.500 208 127 1 8 212 121 328 4.30e-23 102
Msa1390700 MtrunA17_Chr2g0325241 39.521 167 98 1 1 164 142 308 5.91e-18 86.7
Msa1390700 MtrunA17_Chr2g0325311 73.684 114 26 1 482 595 11 120 1.88e-48 164
Msa1390700 MtrunA17_Chr2g0325271 73.737 99 26 0 496 594 45 143 3.52e-42 148
Msa1390700 MtrunA17_Chr8g0361651 36.896 393 203 10 216 595 50 410 1.59e-40 154
Msa1390700 MtrunA17_Chr3g0140591 54.321 162 73 1 334 495 7 167 6.75e-40 143
Msa1390700 MtrunA17_Chr2g0325221 37.942 311 179 2 29 325 275 585 2.89e-39 153
Msa1390700 MtrunA17_Chr2g0325221 38.567 293 169 3 9 301 308 589 5.33e-31 128
Msa1390700 MtrunA17_Chr2g0325221 37.597 258 150 2 16 262 343 600 1.75e-28 120
Msa1390700 MtrunA17_Chr2g0325221 60.204 98 38 1 496 593 603 699 2.25e-26 114
Msa1390700 MtrunA17_Chr2g0325221 38.914 221 117 4 1 207 370 586 1.36e-24 108
Msa1390700 MtrunA17_Chr2g0325221 35.470 234 140 2 103 325 272 505 2.40e-17 85.9
Msa1390700 MtrunA17_Chr2g0325221 37.281 228 129 4 107 325 269 491 3.62e-17 85.5
Msa1390700 MtrunA17_Chr7g0235901 51.515 198 79 2 53 250 131 311 3.89e-35 134
Msa1390700 MtrunA17_Chr7g0235901 46.409 181 86 2 103 279 132 305 8.05e-26 108
Msa1390700 MtrunA17_Chr7g0235901 40.909 198 99 2 1 194 128 311 1.29e-23 101
Msa1390700 MtrunA17_Chr7g0235901 37.968 187 105 2 145 327 132 311 4.92e-19 88.2
Msa1390700 MtrunA17_Chr7g0235901 40.994 161 78 5 179 327 100 255 3.47e-13 70.9
Msa1390700 MtrunA17_Chr2g0325651 56.494 154 43 2 2 134 238 388 4.69e-35 136
Msa1390700 MtrunA17_Chr2g0325651 39.113 248 135 5 33 265 140 386 9.89e-25 106
Msa1390700 MtrunA17_Chr2g0325651 37.302 252 131 4 3 230 138 386 8.29e-23 100
Msa1390700 MtrunA17_Chr2g0325651 39.779 181 85 3 1 174 223 386 3.58e-20 92.8
Msa1390700 MtrunA17_Chr2g0325651 38.462 143 74 2 196 331 236 371 4.74e-13 71.2
Msa1390700 MtrunA17_Chr4g0024061 38.645 251 111 5 353 589 427 648 2.22e-34 139
Msa1390700 MtrunA17_Chr2g0325721 44.082 245 116 2 33 277 278 501 3.12e-30 124
Msa1390700 MtrunA17_Chr2g0325721 38.596 228 112 1 1 200 274 501 2.14e-23 103
Msa1390700 MtrunA17_Chr2g0325721 39.130 276 143 4 38 305 243 501 6.06e-23 102
Msa1390700 MtrunA17_Chr2g0325721 36.975 238 141 2 108 338 243 478 2.02e-16 82.4
Msa1390700 MtrunA17_Chr2g0325721 34.884 215 122 3 142 342 251 461 1.80e-12 70.1
Msa1390700 MtrunA17_Chr4g0044221 31.746 315 172 8 130 431 6 290 1.12e-24 105
Msa1390700 MtrunA17_Chr2g0325751 48.649 148 56 4 192 338 3 131 2.21e-24 99.8
Msa1390700 MtrunA17_Chr2g0325751 38.636 132 74 1 79 210 2 126 2.50e-14 70.9
Msa1390700 MtrunA17_Chr2g0325751 42.017 119 62 1 110 228 5 116 9.35e-14 69.3
Msa1390700 MtrunA17_Chr2g0325751 42.742 124 57 2 136 259 3 112 1.25e-12 65.9
Msa1390700 MtrunA17_Chr2g0325751 39.316 117 64 1 42 158 7 116 2.64e-12 65.1
Msa1390700 MtrunA17_Chr2g0325751 38.261 115 64 1 14 128 7 114 2.19e-11 62.4
Msa1390700 MtrunA17_Chr7g0235921 67.010 97 25 2 414 504 1 96 1.03e-19 84.7
Msa1390700 MtrunA17_Chr2g0325261 49.091 110 52 1 115 224 201 306 1.09e-17 84.3
Msa1390700 MtrunA17_Chr2g0325261 52.500 80 38 0 5 84 227 306 2.71e-12 68.2
Msa1390700 MtrunA17_Chr2g0325341 49.091 110 52 1 115 224 201 306 1.09e-17 84.3
Msa1390700 MtrunA17_Chr2g0325341 52.500 80 38 0 5 84 227 306 2.71e-12 68.2
Msa1390700 MtrunA17_Chr2g0325211 38.202 178 98 2 149 326 2 167 1.70e-16 78.2
Msa1390700 MtrunA17_Chr2g0325791 39.355 155 82 3 43 193 24 170 4.64e-16 79.7
Msa1390700 MtrunA17_Chr2g0325791 41.791 134 68 1 1 134 37 160 1.78e-15 77.8
Msa1390700 MtrunA17_Chr2g0325791 37.681 138 61 3 145 278 41 157 1.42e-14 75.1
Msa1390700 MtrunA17_Chr2g0325791 38.583 127 73 2 85 207 24 149 2.07e-13 71.6
Msa1390700 MtrunA17_Chr2g0325791 39.552 134 76 2 99 228 24 156 2.42e-11 65.5
Msa1390700 MtrunA17_Chr2g0325791 36.242 149 78 3 72 220 38 169 4.90e-11 64.3
Msa1390700 MtrunA17_Chr2g0325781 48.598 107 50 2 43 145 197 302 2.54e-15 77.0
Msa1390700 MtrunA17_Chr2g0325781 51.685 89 39 1 103 187 214 302 2.63e-14 74.3
Msa1390700 MtrunA17_Chr5g0434741 37.037 189 94 5 32 213 125 295 4.87e-12 67.4
Msa1390700 MtrunA17_Chr2g0325731 58.929 56 23 0 84 139 1 56 1.27e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1390700 AT5G27220.1 26.824 466 298 14 34 494 373 800 1.65e-11 68.2
Msa1390700 AT5G27220.3 26.824 466 298 14 34 494 373 800 1.65e-11 68.2
Msa1390700 AT5G27220.4 26.824 466 298 14 34 494 373 800 2.08e-11 67.8
Msa1390700 AT5G27220.2 26.824 466 298 14 34 494 373 800 2.08e-11 67.8

Find 101 sgRNAs with CRISPR-Local

Find 203 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAGAGTTATCTTTATTTCT+TGG 0.198475 tig0021369:+116969 Msa1390700:three_prime_UTR
GCCATCAAGTTGCTTCTCTT+TGG 0.237832 tig0021369:-113663 None:intergenic
CACAAATCAGTCGGGCATTC+TGG 0.239397 tig0021369:-116499 None:intergenic
GAAAATTCTTTGGCTGTTCT+TGG 0.258100 tig0021369:+115737 Msa1390700:CDS
CGTGGAGCTGTTTGGAAGCC+TGG 0.258716 tig0021369:+115856 Msa1390700:CDS
AGAAAATACTGAAAATTCTT+TGG 0.265178 tig0021369:+115727 Msa1390700:CDS
TCAAAAGATAACAAACTTAT+AGG 0.279836 tig0021369:+113788 Msa1390700:CDS
AGATCCATCAAAATTTGTTT+TGG 0.283657 tig0021369:+115532 Msa1390700:CDS
AACTTGTAACTACTATTGTT+TGG 0.296252 tig0021369:+116857 Msa1390700:three_prime_UTR
TGGACTCAAGCTCCTTCATT+TGG 0.314622 tig0021369:-113475 None:intergenic
TTGACTCAAGCTCCTTCATT+TGG 0.318173 tig0021369:-113895 None:intergenic
TCAATACCTGCTCCTCCATT+TGG 0.333415 tig0021369:-113433 None:intergenic
TTGACTCTAATTCCTTCAAT+TGG 0.338034 tig0021369:-113769 None:intergenic
AAAATTTGATGGACAAATAA+AGG 0.345294 tig0021369:+114156 Msa1390700:CDS
GTCTTCAAGTTGCTTCGCTT+TGG 0.345541 tig0021369:-113495 None:intergenic
ATTTGAGGATCTGCTTCTTA+AGG 0.354835 tig0021369:+116735 Msa1390700:CDS
TTACCCCGTTTCTTTGCATT+AGG 0.361827 tig0021369:-116822 None:intergenic
GTTGTTGTAGCAGATAGATT+TGG 0.368863 tig0021369:-115622 None:intergenic
GACTGTCCTACAATGCATTT+CGG 0.382566 tig0021369:+116699 Msa1390700:CDS
ACTTGTAACTACTATTGTTT+GGG 0.393650 tig0021369:+116858 Msa1390700:three_prime_UTR
AAGAAGAACCTTGATTTCAA+TGG 0.397469 tig0021369:-116546 None:intergenic
ACAACCTAATGCAAAGAAAC+GGG 0.400707 tig0021369:+116818 Msa1390700:three_prime_UTR
TTGAGTCAAGCTCCCTCATT+CGG 0.403790 tig0021369:-113265 None:intergenic
GCTATTGAAAGGACAACTTC+AGG 0.410723 tig0021369:+113631 Msa1390700:CDS
CGTTATTCCACTGTGTCAAA+AGG 0.410783 tig0021369:+115571 Msa1390700:CDS
TTGATTCATGCTCCTGCAAT+CGG 0.412660 tig0021369:-113517 None:intergenic
TCCAAAGAGAAGCAACTTGA+TGG 0.413296 tig0021369:+113662 Msa1390700:CDS
GAAATTTCCGTGGAGCTGTT+TGG 0.418240 tig0021369:+115848 Msa1390700:CDS
TGTAATCCGAAATGCATTGT+AGG 0.425499 tig0021369:-116705 None:intergenic
GAAGCTAGAGCATGATTTGA+AGG 0.429517 tig0021369:+115694 Msa1390700:CDS
ACTAGCAATTTCTTCATCTC+TGG 0.433462 tig0021369:-116671 None:intergenic
GTTGTTTGAAATACAAGTTA+AGG 0.433928 tig0021369:+113337 Msa1390700:CDS
AACAACCTAATGCAAAGAAA+CGG 0.436437 tig0021369:+116817 Msa1390700:three_prime_UTR
TTAAAAGAAAAGCAGTTTGA+AGG 0.444691 tig0021369:+114103 Msa1390700:CDS
AACCTTGATTTCAATGGAGT+TGG 0.448151 tig0021369:-116540 None:intergenic
GCAACTTGAAGACCGATTGC+AGG 0.449322 tig0021369:+113505 Msa1390700:CDS
ATTCAGTTGTGCATATAACT+TGG 0.456342 tig0021369:+116436 Msa1390700:CDS
GAAGTTTGAAACAAAAGAAA+AGG 0.466885 tig0021369:+113820 Msa1390700:CDS
GGAATTTGAATGTCAAATGA+TGG 0.469309 tig0021369:+113841 Msa1390700:CDS
AACCAACTCCATTGAAATCA+AGG 0.469309 tig0021369:+116538 Msa1390700:CDS
TTGATTTGAAGTCCTCAACT+TGG 0.471069 tig0021369:-114000 None:intergenic
CTTGAGCTAAAAGCACATAA+AGG 0.471481 tig0021369:+116775 Msa1390700:CDS
GGAATTTGAAGGCCAATTGA+AGG 0.477761 tig0021369:+113757 Msa1390700:CDS
AATGCAAAGAAACGGGGTAA+TGG 0.480139 tig0021369:+116825 Msa1390700:three_prime_UTR
AGAGTATAAGTTCAAAGTAA+AGG 0.481240 tig0021369:+114066 Msa1390700:CDS
GTGGAGCTGTTTGGAAGCCT+GGG 0.486544 tig0021369:+115857 Msa1390700:CDS
TAAAAGACGATGGAATGAAC+TGG 0.489273 tig0021369:+114036 Msa1390700:CDS
CCATCACTTGTCTTTCAAAG+TGG 0.490789 tig0021369:-113715 None:intergenic
TTGGACACAAGTGAGAAAAC+TGG 0.495029 tig0021369:+115458 Msa1390700:CDS
GGATTACAAACTAGAATTTG+AGG 0.497268 tig0021369:+116720 Msa1390700:CDS
AACTGCTCTCACAAATCAGT+CGG 0.510269 tig0021369:-116508 None:intergenic
TGAATGCCAAATGGAGGAGC+AGG 0.517572 tig0021369:+113427 Msa1390700:CDS
CGATTAAGTCGTACCATTGG+TGG 0.522182 tig0021369:+115422 Msa1390700:CDS
GGAGTTTGAATCAAAAGAGA+GGG 0.527207 tig0021369:+113400 Msa1390700:CDS
TCAAAAGAGAGAAAGTTTGA+AGG 0.531967 tig0021369:+113284 Msa1390700:CDS
CCACTTTGAAAGACAAGTGA+TGG 0.536748 tig0021369:+113715 Msa1390700:CDS
CCAACTTGTAGGCAAAATGA+AGG 0.536838 tig0021369:+113379 Msa1390700:CDS
GGAATTTGAAGAACAAGTGA+AGG 0.541780 tig0021369:+113925 Msa1390700:CDS
TGAAGCTCGAGCGAAGGAGC+TGG 0.541954 tig0021369:+113553 Msa1390700:CDS
GAGGGGATTTGAATGCCAAA+TGG 0.542544 tig0021369:+113418 Msa1390700:CDS
TGGTGGAACAAGTTTGCAGT+TGG 0.544547 tig0021369:+115439 Msa1390700:CDS
TGAAGGTCAAGTGAAGGAGC+AGG 0.546239 tig0021369:+113301 Msa1390700:CDS
ATATGCAGTTGAATCTTACA+TGG 0.546667 tig0021369:+115394 Msa1390700:intron
TAGATTCCAACTCCTTCACT+TGG 0.549470 tig0021369:-113685 None:intergenic
GTTATTCCACTGTGTCAAAA+GGG 0.553830 tig0021369:+115572 Msa1390700:CDS
ACTGCTCTCACAAATCAGTC+GGG 0.558430 tig0021369:-116507 None:intergenic
AGTGAAGGACATTAAATCAA+AGG 0.559641 tig0021369:+113940 Msa1390700:CDS
TAAGTTCAAAGTAAAGGTGA+AGG 0.565515 tig0021369:+114072 Msa1390700:CDS
AGGAGTTTGAATCAAAAGAG+AGG 0.566430 tig0021369:+113399 Msa1390700:CDS
CTGCAAACTTGTTCCACCAA+TGG 0.566622 tig0021369:-115435 None:intergenic
TGATGGCCAAGTGAAGGAGT+TGG 0.567033 tig0021369:+113679 Msa1390700:CDS
GCGTGTTGAAAACCAAATGA+AGG 0.568140 tig0021369:+113883 Msa1390700:CDS
GCATTGTGAAAGCCAAATGA+AGG 0.570742 tig0021369:+113463 Msa1390700:CDS
TGCTATGCACGAAATTTCCG+TGG 0.572144 tig0021369:+115838 Msa1390700:CDS
GGACATTAAATCAAAGGAGG+AGG 0.572694 tig0021369:+113946 Msa1390700:CDS
GGAATTTGAAGCTCGAGCGA+AGG 0.575237 tig0021369:+113547 Msa1390700:CDS
TCAAAATTGAGGCTATTGAA+AGG 0.578553 tig0021369:+113620 Msa1390700:CDS
GATCGATTAAGTCGTACCAT+TGG 0.583636 tig0021369:+115419 Msa1390700:CDS
GAAGCATTTCGAAAGCATGA+TGG 0.585317 tig0021369:+113586 Msa1390700:CDS
TTGTTAAGCATCTTATCAAG+AGG 0.585772 tig0021369:+116390 Msa1390700:intron
GGAGCATGAATCAAAGGAGA+AGG 0.587429 tig0021369:+113526 Msa1390700:CDS
AGGAGCTCAAATCAAAATTG+AGG 0.589256 tig0021369:+113609 Msa1390700:CDS
GGACTTGGTATCAAAACTGA+AGG 0.589908 tig0021369:+113232 Msa1390700:CDS
GAAGGAGCTTGAGTCAAAAG+AGG 0.592567 tig0021369:+113901 Msa1390700:CDS
ATGAAGAAATTGCTAGTCTG+AGG 0.599505 tig0021369:+116677 Msa1390700:CDS
GAGTTTGAATCAAAAGAGAG+GGG 0.602082 tig0021369:+113401 Msa1390700:CDS
GCAACTTGATGGCCAAGTGA+AGG 0.602132 tig0021369:+113673 Msa1390700:CDS
ATTGCAGGAGCATGAATCAA+AGG 0.606422 tig0021369:+113520 Msa1390700:CDS
TGAATGCCAAATGATGGACT+TGG 0.610561 tig0021369:+113217 None:intergenic
AAAGTTTGAAGGTCAAGTGA+AGG 0.611491 tig0021369:+113295 Msa1390700:CDS
AAATAAAGGAGCCCGAGTCA+AGG 0.611737 tig0021369:+114170 Msa1390700:CDS
GAAGGACATTAAATCAAAGG+AGG 0.632909 tig0021369:+113943 Msa1390700:CDS
AGCAGCGATTTAAAAGACGA+TGG 0.641163 tig0021369:+114026 Msa1390700:CDS
GGAGCTTGAGTCAAAAGAGG+AGG 0.641783 tig0021369:+113904 Msa1390700:CDS
CAGGCTTCCAAACAGCTCCA+CGG 0.647974 tig0021369:-115855 None:intergenic
GCATTTCGAAAGCATGATGG+AGG 0.653577 tig0021369:+113589 Msa1390700:CDS
GGGATTTGAATGCCAAATGG+AGG 0.654030 tig0021369:+113421 Msa1390700:CDS
AAACTCTGTTAGCAAAACCC+AGG 0.678318 tig0021369:-115874 None:intergenic
CAACCTAATGCAAAGAAACG+GGG 0.689120 tig0021369:+116819 Msa1390700:three_prime_UTR
GACACAAGTGAGAAAACTGG+CGG 0.706814 tig0021369:+115461 Msa1390700:CDS
TTTGACACAGTGGAATAACG+GGG 0.728582 tig0021369:-115568 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGGTTTTACTATAATATTA+TGG + tig0021369:114498-114517 Msa1390700:intron 15.0%
!! TCATCAAATTATGAAATATA+TGG - tig0021369:114789-114808 None:intergenic 15.0%
!!! TTTACACAAATTTCAATTTT+TGG - tig0021369:115662-115681 None:intergenic 15.0%
!! ATAAGAGTTTATCATATTTA+TGG + tig0021369:116069-116088 Msa1390700:intron 15.0%
!! TCCATATCAAAATAATTATT+CGG + tig0021369:116244-116263 Msa1390700:intron 15.0%
!! TCAAAAGATAACAAACTTAT+AGG + tig0021369:113788-113807 Msa1390700:CDS 20.0%
!!! AAAATTTGATGGACAAATAA+AGG + tig0021369:114156-114175 Msa1390700:CDS 20.0%
!!! AAAATATTTTTTCCTTGACT+CGG - tig0021369:114185-114204 None:intergenic 20.0%
!! AAAATATCATCATCATTAAG+AGG - tig0021369:114277-114296 None:intergenic 20.0%
!!! AAATTAGTTTGAAAGTCATT+GGG - tig0021369:114404-114423 None:intergenic 20.0%
!!! TACATTTTTATGTATCTCTT+AGG + tig0021369:114444-114463 Msa1390700:intron 20.0%
!!! AATTATATTGCAAAAAGTGT+AGG + tig0021369:114592-114611 Msa1390700:intron 20.0%
!! TAAATGTACTTTCTATTGTT+GGG - tig0021369:114828-114847 None:intergenic 20.0%
!! ATAAATGTACTTTCTATTGT+TGG - tig0021369:114829-114848 None:intergenic 20.0%
!!! ATATCCAAAACAAATTTTGA+TGG - tig0021369:115539-115558 None:intergenic 20.0%
!! AGAAAATACTGAAAATTCTT+TGG + tig0021369:115727-115746 Msa1390700:CDS 20.0%
!!! AATTATTTTGATATGGAATG+TGG - tig0021369:116241-116260 None:intergenic 20.0%
!!! TCCGAATAATTATTTTGATA+TGG - tig0021369:116248-116267 None:intergenic 20.0%
!! GTTGTTTGAAATACAAGTTA+AGG + tig0021369:113337-113356 Msa1390700:CDS 25.0%
! TCAAAAAATAACCAACTTGT+AGG + tig0021369:113368-113387 Msa1390700:CDS 25.0%
! TCAAAAAAGAAGGAATTTGA+AGG + tig0021369:113746-113765 Msa1390700:CDS 25.0%
!! GAAGTTTGAAACAAAAGAAA+AGG + tig0021369:113820-113839 Msa1390700:CDS 25.0%
! AGAGTATAAGTTCAAAGTAA+AGG + tig0021369:114066-114085 Msa1390700:CDS 25.0%
! TTAAAAGAAAAGCAGTTTGA+AGG + tig0021369:114103-114122 Msa1390700:CDS 25.0%
! TCAATGCTGAAAAAATTTGA+TGG + tig0021369:114145-114164 Msa1390700:CDS 25.0%
!!! AAATATTTTTTCCTTGACTC+GGG - tig0021369:114184-114203 None:intergenic 25.0%
!! GAGTCAAGGAAAAAATATTT+TGG + tig0021369:114184-114203 Msa1390700:CDS 25.0%
!!! TCAAGGAAAAAATATTTTGG+TGG + tig0021369:114187-114206 Msa1390700:CDS 25.0%
!!! CAAGGAAAAAATATTTTGGT+GGG + tig0021369:114188-114207 Msa1390700:CDS 25.0%
!!! AAGGAAAAAATATTTTGGTG+GGG + tig0021369:114189-114208 Msa1390700:CDS 25.0%
! TAGATGAATAAGACATTTAC+CGG - tig0021369:114224-114243 None:intergenic 25.0%
!! CAAATTAGTTTGAAAGTCAT+TGG - tig0021369:114405-114424 None:intergenic 25.0%
! AGATACATAAAAATGTACAG+GGG - tig0021369:114441-114460 None:intergenic 25.0%
! GAGATACATAAAAATGTACA+GGG - tig0021369:114442-114461 None:intergenic 25.0%
! AGAGATACATAAAAATGTAC+AGG - tig0021369:114443-114462 None:intergenic 25.0%
! AATATTCTGACAAACATTGA+GGG + tig0021369:114618-114637 Msa1390700:intron 25.0%
! TTTATGAGTTCATGTACAAA+AGG + tig0021369:114844-114863 Msa1390700:intron 25.0%
!!! CTATGTAGTTTTGTATAACA+TGG + tig0021369:115012-115031 Msa1390700:intron 25.0%
! TACTAAATATTGGAATGAAC+TGG - tig0021369:115219-115238 None:intergenic 25.0%
!!! AGATCCATCAAAATTTGTTT+TGG + tig0021369:115532-115551 Msa1390700:CDS 25.0%
!!! ACAAATTTCAATTTTTGGTG+AGG - tig0021369:115657-115676 None:intergenic 25.0%
!!! TGCTGCTTTTATCAATTTAT+GGG + tig0021369:115765-115784 Msa1390700:CDS 25.0%
!! TTTTAACGAAGATGAAGTTT+TGG + tig0021369:115799-115818 Msa1390700:CDS 25.0%
! GATTATCAAATCAAATGAGT+TGG + tig0021369:115937-115956 Msa1390700:intron 25.0%
! TATCATATTTATGGTGAACA+TGG + tig0021369:116078-116097 Msa1390700:intron 25.0%
! TCAAAATTTGTCAACCATAT+AGG - tig0021369:116280-116299 None:intergenic 25.0%
! ATAAGATGCTTAACAAAATC+AGG - tig0021369:116386-116405 None:intergenic 25.0%
!! TATATTCCATTTTCACACAT+AGG + tig0021369:116594-116613 Msa1390700:intron 25.0%
! AACTTGTAACTACTATTGTT+TGG + tig0021369:116857-116876 Msa1390700:three_prime_UTR 25.0%
! ACTTGTAACTACTATTGTTT+GGG + tig0021369:116858-116877 Msa1390700:three_prime_UTR 25.0%
! ACTCTGTAAAAAGATGTAAT+TGG + tig0021369:116919-116938 Msa1390700:three_prime_UTR 25.0%
!! ACAGAGTTATCTTTATTTCT+TGG + tig0021369:116969-116988 Msa1390700:three_prime_UTR 25.0%
TCAAAAGAGAGAAAGTTTGA+AGG + tig0021369:113284-113303 Msa1390700:CDS 30.0%
TCAAAATTGAGGCTATTGAA+AGG + tig0021369:113620-113639 Msa1390700:CDS 30.0%
TTGACTCTAATTCCTTCAAT+TGG - tig0021369:113772-113791 None:intergenic 30.0%
GGAATTTGAATGTCAAATGA+TGG + tig0021369:113841-113860 Msa1390700:CDS 30.0%
AGTGAAGGACATTAAATCAA+AGG + tig0021369:113940-113959 Msa1390700:CDS 30.0%
!! GAATTTTAAAAGCCAAGTTG+AGG + tig0021369:113988-114007 Msa1390700:CDS 30.0%
TAAGTTCAAAGTAAAGGTGA+AGG + tig0021369:114072-114091 Msa1390700:CDS 30.0%
!!! AATTTTTTCAGCATTGACTC+AGG - tig0021369:114142-114161 None:intergenic 30.0%
!!! AACTCGTGTTTTTTTATCCA+CGG + tig0021369:114473-114492 Msa1390700:intron 30.0%
CAATATTCTGACAAACATTG+AGG + tig0021369:114617-114636 Msa1390700:intron 30.0%
ATATTCTGACAAACATTGAG+GGG + tig0021369:114619-114638 Msa1390700:intron 30.0%
CTAGAAATAGTAGCACTAAT+AGG - tig0021369:114664-114683 None:intergenic 30.0%
! TTTTTCACTGCAATCATTCT+AGG - tig0021369:114975-114994 None:intergenic 30.0%
! TTTTGTATAACATGGCAGAT+TGG + tig0021369:115020-115039 Msa1390700:intron 30.0%
TGAACGTTACAGATAATCTA+GGG - tig0021369:115164-115183 None:intergenic 30.0%
TTGAACGTTACAGATAATCT+AGG - tig0021369:115165-115184 None:intergenic 30.0%
GCTGCACTTATACTAAATAT+TGG - tig0021369:115229-115248 None:intergenic 30.0%
TGTATGACATTGATGAGTTA+CGG - tig0021369:115251-115270 None:intergenic 30.0%
ATATGCAGTTGAATCTTACA+TGG + tig0021369:115394-115413 Msa1390700:intron 30.0%
!!! CTGCTGCTTTTATCAATTTA+TGG + tig0021369:115764-115783 Msa1390700:CDS 30.0%
TCTAAGAAACTAGCAACTTA+TGG - tig0021369:116037-116056 None:intergenic 30.0%
TATTTATGGTGAACATGGTA+TGG + tig0021369:116083-116102 Msa1390700:intron 30.0%
TTGTTAAGCATCTTATCAAG+AGG + tig0021369:116390-116409 Msa1390700:intron 30.0%
ATTCAGTTGTGCATATAACT+TGG + tig0021369:116436-116455 Msa1390700:CDS 30.0%
AAGAAGAACCTTGATTTCAA+TGG - tig0021369:116549-116568 None:intergenic 30.0%
GGATTACAAACTAGAATTTG+AGG + tig0021369:116720-116739 Msa1390700:CDS 30.0%
AACAACCTAATGCAAAGAAA+CGG + tig0021369:116817-116836 Msa1390700:three_prime_UTR 30.0%
!! AGGATTTTGAAAACCGAATG+AGG + tig0021369:113252-113271 Msa1390700:CDS 35.0%
!! GGATTTTGAAAACCGAATGA+GGG + tig0021369:113253-113272 Msa1390700:CDS 35.0%
AAAGTTTGAAGGTCAAGTGA+AGG + tig0021369:113295-113314 Msa1390700:CDS 35.0%
AGGAGTTTGAATCAAAAGAG+AGG + tig0021369:113399-113418 Msa1390700:CDS 35.0%
GGAGTTTGAATCAAAAGAGA+GGG + tig0021369:113400-113419 Msa1390700:CDS 35.0%
GAGTTTGAATCAAAAGAGAG+GGG + tig0021369:113401-113420 Msa1390700:CDS 35.0%
AGGAGCTCAAATCAAAATTG+AGG + tig0021369:113609-113628 Msa1390700:CDS 35.0%
GGAGTTTGAGTCAAAAAAGA+AGG + tig0021369:113736-113755 Msa1390700:CDS 35.0%
GGAATTTGAAGAACAAGTGA+AGG + tig0021369:113925-113944 Msa1390700:CDS 35.0%
GAAGGACATTAAATCAAAGG+AGG + tig0021369:113943-113962 Msa1390700:CDS 35.0%
GGAGGAACTTGAATCAAAAA+AGG + tig0021369:113964-113983 Msa1390700:CDS 35.0%
! TTGATTTGAAGTCCTCAACT+TGG - tig0021369:114003-114022 None:intergenic 35.0%
TAAAAGACGATGGAATGAAC+TGG + tig0021369:114036-114055 Msa1390700:CDS 35.0%
! TTGGGTGTGATAATGAAATC+TGG - tig0021369:114386-114405 None:intergenic 35.0%
!!! TGTTTTTTTATCCACGGCTA+AGG + tig0021369:114479-114498 Msa1390700:intron 35.0%
TTATAGTAAAACCTTAGCCG+TGG - tig0021369:114493-114512 None:intergenic 35.0%
AGTAGCACTAATAGGACTAA+TGG - tig0021369:114656-114675 None:intergenic 35.0%
GTTTCAACATAGACCTTAAC+TGG - tig0021369:114730-114749 None:intergenic 35.0%
GAGAGTCTAGACATAAATAG+AGG - tig0021369:114911-114930 None:intergenic 35.0%
AGCAAAATAAGCAGTACAAG+TGG + tig0021369:114938-114957 Msa1390700:intron 35.0%
TTGCAAAACAAGCTCTTCAT+TGG - tig0021369:115060-115079 None:intergenic 35.0%
AATTCTGTCCAGTTGTATAG+AGG - tig0021369:115103-115122 None:intergenic 35.0%
AGATAATCTAGGGAAAGTCA+TGG - tig0021369:115154-115173 None:intergenic 35.0%
TATTGGAATGAACTGGATCT+TGG - tig0021369:115212-115231 None:intergenic 35.0%
! TTTTGACACAGTGGAATAAC+GGG - tig0021369:115572-115591 None:intergenic 35.0%
! CTTTTGACACAGTGGAATAA+CGG - tig0021369:115573-115592 None:intergenic 35.0%
GTTATTCCACTGTGTCAAAA+GGG + tig0021369:115572-115591 Msa1390700:CDS 35.0%
GTTGTTGTAGCAGATAGATT+TGG - tig0021369:115625-115644 None:intergenic 35.0%
GAAAATTCTTTGGCTGTTCT+TGG + tig0021369:115737-115756 Msa1390700:CDS 35.0%
!!! TTTCAATGGAGTTGGTTTTC+AGG - tig0021369:116535-116554 None:intergenic 35.0%
AACCAACTCCATTGAAATCA+AGG + tig0021369:116538-116557 Msa1390700:CDS 35.0%
AACCTTGATTTCAATGGAGT+TGG - tig0021369:116543-116562 None:intergenic 35.0%
TGCTATCCTATGTGTGAAAA+TGG - tig0021369:116603-116622 None:intergenic 35.0%
GTTACTGTATACTTGAACTC+AGG + tig0021369:116642-116661 Msa1390700:intron 35.0%
GTATACTTGAACTCAGGATA+AGG + tig0021369:116648-116667 Msa1390700:intron 35.0%
ACTAGCAATTTCTTCATCTC+TGG - tig0021369:116674-116693 None:intergenic 35.0%
! ATGAAGAAATTGCTAGTCTG+AGG + tig0021369:116677-116696 Msa1390700:CDS 35.0%
TGTAATCCGAAATGCATTGT+AGG - tig0021369:116708-116727 None:intergenic 35.0%
ATTTGAGGATCTGCTTCTTA+AGG + tig0021369:116735-116754 Msa1390700:CDS 35.0%
! CTTGAGCTAAAAGCACATAA+AGG + tig0021369:116775-116794 Msa1390700:CDS 35.0%
ACAACCTAATGCAAAGAAAC+GGG + tig0021369:116818-116837 Msa1390700:three_prime_UTR 35.0%
! GGACTTGGTATCAAAACTGA+AGG + tig0021369:113232-113251 Msa1390700:CDS 40.0%
! CCTTCATTTTGCCTACAAGT+TGG - tig0021369:113382-113401 None:intergenic 40.0%
CCAACTTGTAGGCAAAATGA+AGG + tig0021369:113379-113398 Msa1390700:CDS 40.0%
GCATTGTGAAAGCCAAATGA+AGG + tig0021369:113463-113482 Msa1390700:CDS 40.0%
TTGATTCATGCTCCTGCAAT+CGG - tig0021369:113520-113539 None:intergenic 40.0%
! ATTGCAGGAGCATGAATCAA+AGG + tig0021369:113520-113539 Msa1390700:CDS 40.0%
! GAAGCATTTCGAAAGCATGA+TGG + tig0021369:113586-113605 Msa1390700:CDS 40.0%
GCTATTGAAAGGACAACTTC+AGG + tig0021369:113631-113650 Msa1390700:CDS 40.0%
TCCAAAGAGAAGCAACTTGA+TGG + tig0021369:113662-113681 Msa1390700:CDS 40.0%
TAGATTCCAACTCCTTCACT+TGG - tig0021369:113688-113707 None:intergenic 40.0%
CCATCACTTGTCTTTCAAAG+TGG - tig0021369:113718-113737 None:intergenic 40.0%
! CCACTTTGAAAGACAAGTGA+TGG + tig0021369:113715-113734 Msa1390700:CDS 40.0%
GGAATTTGAAGGCCAATTGA+AGG + tig0021369:113757-113776 Msa1390700:CDS 40.0%
GCGTGTTGAAAACCAAATGA+AGG + tig0021369:113883-113902 Msa1390700:CDS 40.0%
TTGACTCAAGCTCCTTCATT+TGG - tig0021369:113898-113917 None:intergenic 40.0%
GGACATTAAATCAAAGGAGG+AGG + tig0021369:113946-113965 Msa1390700:CDS 40.0%
AGCAGCGATTTAAAAGACGA+TGG + tig0021369:114026-114045 Msa1390700:CDS 40.0%
TTTGGTGGGGAAAAAGAATC+CGG + tig0021369:114202-114221 Msa1390700:CDS 40.0%
ACTAACACTGTCTCCAGTTA+AGG + tig0021369:114714-114733 Msa1390700:intron 40.0%
TCAACCTTCCTCTATACAAC+TGG + tig0021369:115092-115111 Msa1390700:intron 40.0%
GATCGATTAAGTCGTACCAT+TGG + tig0021369:115419-115438 Msa1390700:CDS 40.0%
TTGGACACAAGTGAGAAAAC+TGG + tig0021369:115458-115477 Msa1390700:CDS 40.0%
TTTGACACAGTGGAATAACG+GGG - tig0021369:115571-115590 None:intergenic 40.0%
CGTTATTCCACTGTGTCAAA+AGG + tig0021369:115571-115590 Msa1390700:CDS 40.0%
GAAGCTAGAGCATGATTTGA+AGG + tig0021369:115694-115713 Msa1390700:CDS 40.0%
AAACTCTGTTAGCAAAACCC+AGG - tig0021369:115877-115896 None:intergenic 40.0%
! GGGTTTTGCTAACAGAGTTT+CGG + tig0021369:115877-115896 Msa1390700:intron 40.0%
! GGTTTTGCTAACAGAGTTTC+GGG + tig0021369:115878-115897 Msa1390700:intron 40.0%
GACTGCATATAGGAATGGTA+GGG - tig0021369:115979-115998 None:intergenic 40.0%
TGACTGCATATAGGAATGGT+AGG - tig0021369:115980-115999 None:intergenic 40.0%
TACCATTCCTATATGCAGTC+AGG + tig0021369:115979-115998 Msa1390700:intron 40.0%
ATCCTGACTGCATATAGGAA+TGG - tig0021369:115984-116003 None:intergenic 40.0%
!!! TTTGCCCTGTTTTTGTCCTA+CGG + tig0021369:116196-116215 Msa1390700:intron 40.0%
AGAACCGTAGGACAAAAACA+GGG - tig0021369:116203-116222 None:intergenic 40.0%
TAGAACCGTAGGACAAAAAC+AGG - tig0021369:116204-116223 None:intergenic 40.0%
! GTTTTTGTCCTACGGTTCTA+TGG + tig0021369:116204-116223 Msa1390700:intron 40.0%
ATTGTGATCCATAGAACCGT+AGG - tig0021369:116215-116234 None:intergenic 40.0%
TGTCAACCATATAGGCGATT+TGG - tig0021369:116272-116291 None:intergenic 40.0%
AACTGCTCTCACAAATCAGT+CGG - tig0021369:116511-116530 None:intergenic 40.0%
GACTGTCCTACAATGCATTT+CGG + tig0021369:116699-116718 Msa1390700:CDS 40.0%
CAACCTAATGCAAAGAAACG+GGG + tig0021369:116819-116838 Msa1390700:three_prime_UTR 40.0%
TTACCCCGTTTCTTTGCATT+AGG - tig0021369:116825-116844 None:intergenic 40.0%
AATGCAAAGAAACGGGGTAA+TGG + tig0021369:116825-116844 Msa1390700:three_prime_UTR 40.0%
TTGAGTCAAGCTCCCTCATT+CGG - tig0021369:113268-113287 None:intergenic 45.0%
GAGGGGATTTGAATGCCAAA+TGG + tig0021369:113418-113437 Msa1390700:CDS 45.0%
GGGATTTGAATGCCAAATGG+AGG + tig0021369:113421-113440 Msa1390700:CDS 45.0%
TCAATACCTGCTCCTCCATT+TGG - tig0021369:113436-113455 None:intergenic 45.0%
TGGACTCAAGCTCCTTCATT+TGG - tig0021369:113478-113497 None:intergenic 45.0%
!! GTCTTCAAGTTGCTTCGCTT+TGG - tig0021369:113498-113517 None:intergenic 45.0%
GGAGCATGAATCAAAGGAGA+AGG + tig0021369:113526-113545 Msa1390700:CDS 45.0%
! GCATTTCGAAAGCATGATGG+AGG + tig0021369:113589-113608 Msa1390700:CDS 45.0%
! GCCATCAAGTTGCTTCTCTT+TGG - tig0021369:113666-113685 None:intergenic 45.0%
GAAGGAGCTTGAGTCAAAAG+AGG + tig0021369:113901-113920 Msa1390700:CDS 45.0%
AAATAAAGGAGCCCGAGTCA+AGG + tig0021369:114170-114189 Msa1390700:CDS 45.0%
CTGTCCAGTTGTATAGAGGA+AGG - tig0021369:115099-115118 None:intergenic 45.0%
CTAGGGAAAGTCATGGAACT+TGG - tig0021369:115147-115166 None:intergenic 45.0%
CGATTAAGTCGTACCATTGG+TGG + tig0021369:115422-115441 Msa1390700:CDS 45.0%
! CTGCAAACTTGTTCCACCAA+TGG - tig0021369:115438-115457 None:intergenic 45.0%
TGGTGGAACAAGTTTGCAGT+TGG + tig0021369:115439-115458 Msa1390700:CDS 45.0%
GACACAAGTGAGAAAACTGG+CGG + tig0021369:115461-115480 Msa1390700:CDS 45.0%
! TTGTCACCCTTTTGACACAG+TGG - tig0021369:115581-115600 None:intergenic 45.0%
TGCTATGCACGAAATTTCCG+TGG + tig0021369:115838-115857 Msa1390700:CDS 45.0%
GAAATTTCCGTGGAGCTGTT+TGG + tig0021369:115848-115867 Msa1390700:CDS 45.0%
TCCTATATGCAGTCAGGATG+AGG + tig0021369:115985-116004 Msa1390700:intron 45.0%
ACCTCATCCTGACTGCATAT+AGG - tig0021369:115989-116008 None:intergenic 45.0%
TATATGCAGTCAGGATGAGG+TGG + tig0021369:115988-116007 Msa1390700:intron 45.0%
TCGGAACCAAATCGCCTATA+TGG + tig0021369:116263-116282 Msa1390700:intron 45.0%
ACTGCTCTCACAAATCAGTC+GGG - tig0021369:116510-116529 None:intergenic 45.0%
TGAAGGTCAAGTGAAGGAGC+AGG + tig0021369:113301-113320 Msa1390700:CDS 50.0%
TGAATGCCAAATGGAGGAGC+AGG + tig0021369:113427-113446 Msa1390700:CDS 50.0%
GCAACTTGAAGACCGATTGC+AGG + tig0021369:113505-113524 Msa1390700:CDS 50.0%
! GGAATTTGAAGCTCGAGCGA+AGG + tig0021369:113547-113566 Msa1390700:CDS 50.0%
! GCAACTTGATGGCCAAGTGA+AGG + tig0021369:113673-113692 Msa1390700:CDS 50.0%
TGATGGCCAAGTGAAGGAGT+TGG + tig0021369:113679-113698 Msa1390700:CDS 50.0%
GGAGCTTGAGTCAAAAGAGG+AGG + tig0021369:113904-113923 Msa1390700:CDS 50.0%
GATCTTGGCGAGGAAAGATG+TGG - tig0021369:115197-115216 None:intergenic 50.0%
GAATGAACTGGATCTTGGCG+AGG - tig0021369:115207-115226 None:intergenic 50.0%
CACAAATCAGTCGGGCATTC+TGG - tig0021369:116502-116521 None:intergenic 50.0%
GCGAGGAAAGATGTGGATGC+TGG - tig0021369:115190-115209 None:intergenic 55.0%
CAGGCTTCCAAACAGCTCCA+CGG - tig0021369:115858-115877 None:intergenic 55.0%
GTGGAGCTGTTTGGAAGCCT+GGG + tig0021369:115857-115876 Msa1390700:CDS 55.0%
TGAAGCTCGAGCGAAGGAGC+TGG + tig0021369:113553-113572 Msa1390700:CDS 60.0%
CGTGGAGCTGTTTGGAAGCC+TGG + tig0021369:115856-115875 Msa1390700:CDS 60.0%
Chromosome Type Strat End Strand Name
tig0021369 gene 113230 117002 113230 ID=Msa1390700;Name=Msa1390700
tig0021369 mRNA 113230 117002 113230 ID=Msa1390700-mRNA-1;Parent=Msa1390700;Name=Msa1390700-mRNA-1;_AED=0.29;_eAED=0.29;_QI=0|0.66|0.25|1|1|1|4|198|595
tig0021369 exon 113230 114223 113230 ID=Msa1390700-mRNA-1:exon:12353;Parent=Msa1390700-mRNA-1
tig0021369 exon 115402 115892 115402 ID=Msa1390700-mRNA-1:exon:12354;Parent=Msa1390700-mRNA-1
tig0021369 exon 116398 116559 116398 ID=Msa1390700-mRNA-1:exon:12355;Parent=Msa1390700-mRNA-1
tig0021369 exon 116664 117002 116664 ID=Msa1390700-mRNA-1:exon:12356;Parent=Msa1390700-mRNA-1
tig0021369 CDS 113230 114223 113230 ID=Msa1390700-mRNA-1:cds;Parent=Msa1390700-mRNA-1
tig0021369 CDS 115402 115892 115402 ID=Msa1390700-mRNA-1:cds;Parent=Msa1390700-mRNA-1
tig0021369 CDS 116398 116559 116398 ID=Msa1390700-mRNA-1:cds;Parent=Msa1390700-mRNA-1
tig0021369 CDS 116664 116804 116664 ID=Msa1390700-mRNA-1:cds;Parent=Msa1390700-mRNA-1
tig0021369 three_prime_UTR 116805 117002 116805 ID=Msa1390700-mRNA-1:three_prime_utr;Parent=Msa1390700-mRNA-1
Gene Sequence

>Msa1390700

ATGGACTTGGTATCAAAACTGAAGGATTTTGAAAACCGAATGAGGGAGCTTGACTCAAAAGAGAGAAAGTTTGAAGGTCAAGTGAAGGAGCAGGAATCTAAAAAGAAGTTGTTTGAAATACAAGTTAAGGAGTTAGAGTCAAAAAATAACCAACTTGTAGGCAAAATGAAGGAGTTTGAATCAAAAGAGAGGGGATTTGAATGCCAAATGGAGGAGCAGGTATTGAAACAGAAGCATTGTGAAAGCCAAATGAAGGAGCTTGAGTCCAAAGCGAAGCAACTTGAAGACCGATTGCAGGAGCATGAATCAAAGGAGAAGGAATTTGAAGCTCGAGCGAAGGAGCTGGAATCTAAAAAGAAGCATTTCGAAAGCATGATGGAGGAGCTCAAATCAAAATTGAGGCTATTGAAAGGACAACTTCAGGAATTTGACTCCAAAGAGAAGCAACTTGATGGCCAAGTGAAGGAGTTGGAATCTAAAAAGAACCACTTTGAAAGACAAGTGATGGAGTTTGAGTCAAAAAAGAAGGAATTTGAAGGCCAATTGAAGGAATTAGAGTCAAAAGATAACAAACTTATAGGCAAAATAAAGAAGTTTGAAACAAAAGAAAAGGAATTTGAATGTCAAATGATGGACTTGCTATCAAATCAGAAGCGTGTTGAAAACCAAATGAAGGAGCTTGAGTCAAAAGAGGAGGAATTTGAAGAACAAGTGAAGGACATTAAATCAAAGGAGGAGGAACTTGAATCAAAAAAGGAGAATTTTAAAAGCCAAGTTGAGGACTTCAAATCAAAAGAGCAGCGATTTAAAAGACGATGGAATGAACTGGAGTTGAAAGAGTATAAGTTCAAAGTAAAGGTGAAGGAGCTCAACTTAAAAGAAAAGCAGTTTGAAGGACAAGTCAACGATCCTGAGTCAATGCTGAAAAAATTTGATGGACAAATAAAGGAGCCCGAGTCAAGGAAAAAATATTTTGGTGGGGAAAAAGAATCCGTTGAATCTTACATGGATGATCGATTAAGTCGTACCATTGGTGGAACAAGTTTGCAGTTGGACACAAGTGAGAAAACTGGCGGAGTTGAATCGCTTTGCAATAGAATTTTAGTTAGTATGCGAGAATCATCAGATCCATCAAAATTTGTTTTGGATATGATATTGAACCCCGTTATTCCACTGTGTCAAAAGGGTGACAATGCTGTGATTATTGCTGATTACCAAATCTATCTGCTACAACAACTGATGAGAACCTCACCAAAAATTGAAATTTGTGTAAAAAAAGAAGCATTGAAGCTAGAGCATGATTTGAAGGCTAACATGAAAGAAAATACTGAAAATTCTTTGGCTGTTCTTGGTTTTCTGCTGCTTTTATCAATTTATGGGTTGCTTGATTATTTTAACGAAGATGAAGTTTTGGAGCTTTTTGCATTTGTTGCTATGCACGAAATTTCCGTGGAGCTGTTTGGAAGCCTGGGTTTTGCTAACAGACATCTTATCAAGAGGAAGCAATTTGTTGAAGCTGTTAGATTCAGTTGTGCATATAACTTGGATGACAAGAATCGATTAGTTGATATGTTGCGAGAACACATCCAGAATGCCCGACTGATTTGTGAGAGCAGTTGCCTGAAAACCAACTCCATTGAAATCAAGGATAAGGCCAGAGATGAAGAAATTGCTAGTCTGAGGACTGTCCTACAATGCATTTCGGATTACAAACTAGAATTTGAGGATCTGCTTCTTAAGGAGATTGAATATCGCATTCTTGAGCTAAAAGCACATAAAGGCAATTAG
Protein sequence

>Msa1390700

MDLVSKLKDFENRMRELDSKERKFEGQVKEQESKKKLFEIQVKELESKNNQLVGKMKEFESKERGFECQMEEQVLKQKHCESQMKELESKAKQLEDRLQEHESKEKEFEARAKELESKKKHFESMMEELKSKLRLLKGQLQEFDSKEKQLDGQVKELESKKNHFERQVMEFESKKKEFEGQLKELESKDNKLIGKIKKFETKEKEFECQMMDLLSNQKRVENQMKELESKEEEFEEQVKDIKSKEEELESKKENFKSQVEDFKSKEQRFKRRWNELELKEYKFKVKVKELNLKEKQFEGQVNDPESMLKKFDGQIKEPESRKKYFGGEKESVESYMDDRLSRTIGGTSLQLDTSEKTGGVESLCNRILVSMRESSDPSKFVLDMILNPVIPLCQKGDNAVIIADYQIYLLQQLMRTSPKIEICVKKEALKLEHDLKANMKENTENSLAVLGFLLLLSIYGLLDYFNEDEVLELFAFVAMHEISVELFGSLGFANRHLIKRKQFVEAVRFSCAYNLDDKNRLVDMLREHIQNARLICESSCLKTNSIEIKDKARDEEIASLRTVLQCISDYKLEFEDLLLKEIEYRILELKAHKGN*