AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0062850

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0062850 MtrunA17_Chr1g0163311 87.121 528 40 3 1 500 1 528 0.0 890
Msa0062850 MtrunA17_Chr4g0037661 51.543 551 199 9 1 500 1 534 8.64e-178 510
Msa0062850 MtrunA17_Chr4g0023801 32.740 562 281 15 4 499 5 535 1.26e-79 258
Msa0062850 MtrunA17_Chr1g0199631 28.571 413 223 12 36 408 27 407 1.00e-39 151
Msa0062850 MtrunA17_Chr2g0279481 26.996 526 271 18 6 451 47 539 2.60e-39 151
Msa0062850 MtrunA17_Chr2g0303541 24.164 538 308 13 36 497 63 576 8.50e-36 140
Msa0062850 MtrunA17_Chr2g0285511 24.832 447 264 12 6 408 29 447 1.77e-35 139
Msa0062850 MtrunA17_Chr5g0401341 24.895 474 299 9 6 451 30 474 4.11e-35 139
Msa0062850 MtrunA17_Chr2g0280541 28.977 352 211 11 67 409 1 322 4.74e-35 136
Msa0062850 MtrunA17_Chr2g0320691 25.792 442 252 14 17 411 36 448 2.07e-34 137
Msa0062850 MtrunA17_Chr8g0374011 29.194 459 245 15 45 454 85 512 6.90e-34 135
Msa0062850 MtrunA17_Chr8g0382231 25.394 508 311 20 5 475 54 530 1.23e-33 134
Msa0062850 MtrunA17_Chr7g0245201 26.763 482 267 15 13 451 70 508 4.87e-33 132
Msa0062850 MtrunA17_Chr1g0195911 26.337 505 262 20 5 452 38 489 1.07e-29 122
Msa0062850 MtrunA17_Chr4g0010091 26.437 435 236 12 17 408 47 440 6.67e-29 120
Msa0062850 MtrunA17_Chr1g0181181 25.110 454 241 14 6 411 29 431 2.69e-28 118
Msa0062850 MtrunA17_Chr1g0191711 27.209 430 232 14 6 411 61 433 1.46e-27 115
Msa0062850 MtrunA17_Chr3g0108331 26.712 438 230 13 14 415 34 416 2.33e-26 112
Msa0062850 MtrunA17_Chr5g0401451 24.540 489 293 15 6 456 43 493 2.37e-26 112
Msa0062850 MtrunA17_Chr1g0207461 25.523 478 297 14 5 451 30 479 2.82e-26 112
Msa0062850 MtrunA17_Chr4g0076521 24.368 435 264 11 6 411 29 427 4.25e-26 112
Msa0062850 MtrunA17_Chr1g0150191 26.952 397 238 12 42 411 69 440 4.47e-26 112
Msa0062850 MtrunA17_Chr7g0268781 25.200 500 282 19 5 454 38 495 6.15e-26 111
Msa0062850 MtrunA17_Chr5g0432861 23.978 538 285 16 14 483 35 516 2.90e-24 106
Msa0062850 MtrunA17_Chr4g0053841 24.795 488 294 15 5 456 15 465 1.98e-23 103
Msa0062850 MtrunA17_Chr2g0290741 24.826 431 253 12 6 409 29 415 2.20e-21 97.4
Msa0062850 MtrunA17_Chr5g0412591 23.969 509 246 17 17 451 39 480 3.00e-21 97.1
Msa0062850 MtrunA17_Chr4g0003891 30.180 222 133 7 210 411 46 265 5.52e-20 92.4
Msa0062850 MtrunA17_Chr4g0050021 30.303 198 118 4 273 451 336 532 1.82e-18 88.6
Msa0062850 MtrunA17_Chr4g0050021 30.472 233 130 7 5 233 46 250 5.67e-18 87.0
Msa0062850 MtrunA17_Chr2g0332281 28.649 185 126 3 272 451 101 284 9.52e-18 85.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0062850 AT3G19850.1 39.964 553 252 17 1 492 1 534 1.79e-112 344
Msa0062850 AT1G50280.1 44.138 435 202 10 1 411 1 418 1.58e-102 317
Msa0062850 AT1G50280.2 45.573 384 177 8 43 411 19 385 1.24e-94 296
Msa0062850 AT3G22104.1 31.507 511 292 15 6 492 7 483 1.02e-65 221
Msa0062850 AT3G22104.4 30.822 438 254 13 72 492 3 408 1.74e-46 167
Msa0062850 AT3G22104.2 30.822 438 254 13 72 492 3 408 1.74e-46 167
Msa0062850 AT3G22104.3 30.822 438 254 13 72 492 3 408 1.74e-46 167
Msa0062850 AT3G08570.1 25.835 569 296 21 13 499 43 567 9.70e-38 146
Msa0062850 AT1G30440.1 27.234 470 254 14 36 451 64 499 1.01e-36 144
Msa0062850 AT3G08660.1 25.906 552 297 17 9 499 39 539 1.28e-36 143
Msa0062850 AT3G08660.2 28.341 434 234 14 9 415 39 422 4.08e-36 139
Msa0062850 AT5G03250.1 25.179 560 320 19 6 495 29 559 4.12e-36 142
Msa0062850 AT5G66560.3 29.167 432 240 12 44 438 78 480 1.77e-34 137
Msa0062850 AT5G66560.4 29.167 432 240 12 44 438 68 470 1.90e-34 137
Msa0062850 AT5G66560.1 29.167 432 240 12 44 438 107 509 2.11e-34 137
Msa0062850 AT5G66560.2 29.777 403 217 12 44 409 107 480 4.51e-34 134
Msa0062850 AT5G48800.1 27.739 429 245 11 6 408 44 433 6.51e-34 135
Msa0062850 AT3G08660.3 25.379 528 285 16 30 499 3 479 3.52e-33 132
Msa0062850 AT3G44820.1 25.380 461 264 14 6 411 26 461 2.79e-32 131
Msa0062850 AT5G64330.2 25.175 572 272 20 6 451 55 596 7.46e-32 130
Msa0062850 AT5G64330.1 25.175 572 272 20 6 451 55 596 9.20e-32 130
Msa0062850 AT5G64330.3 24.956 565 269 19 13 451 2 537 1.44e-31 128
Msa0062850 AT3G50840.1 27.605 547 306 16 6 500 19 527 2.91e-30 124
Msa0062850 AT3G26490.2 25.658 456 260 15 6 409 29 457 5.41e-30 123
Msa0062850 AT2G47860.1 25.586 469 250 18 6 426 40 457 8.92e-30 123
Msa0062850 AT3G26490.1 25.658 456 260 15 6 409 29 457 9.16e-30 123
Msa0062850 AT3G26490.3 25.658 456 260 15 6 409 29 457 9.16e-30 123
Msa0062850 AT3G26490.4 25.658 456 260 15 6 409 29 457 9.16e-30 123
Msa0062850 AT5G13600.1 26.134 551 318 21 6 495 29 551 1.07e-29 123
Msa0062850 AT3G50840.2 27.533 523 290 15 30 500 6 491 5.97e-29 120
Msa0062850 AT3G50840.3 27.533 523 290 15 30 500 6 491 5.97e-29 120
Msa0062850 AT2G47860.4 25.885 452 240 17 6 409 40 444 6.34e-29 119
Msa0062850 AT5G67440.3 27.296 392 228 11 42 411 72 428 1.87e-28 119
Msa0062850 AT5G67440.1 27.296 392 228 11 42 411 72 428 1.87e-28 119
Msa0062850 AT5G67440.2 27.296 392 228 11 42 411 35 391 2.18e-28 119
Msa0062850 AT2G47860.6 26.364 440 229 17 18 409 75 467 3.36e-28 117
Msa0062850 AT5G47800.3 26.608 451 235 18 6 411 29 428 7.85e-28 117
Msa0062850 AT5G47800.1 26.608 451 235 18 6 411 29 428 7.85e-28 117
Msa0062850 AT5G47800.4 26.608 451 235 18 6 411 29 428 7.85e-28 117
Msa0062850 AT5G47800.2 26.608 451 235 18 6 411 142 541 2.06e-27 116
Msa0062850 AT4G37590.1 25.577 477 276 15 5 451 29 456 1.80e-26 113
Msa0062850 AT5G67385.1 24.380 484 296 15 6 456 28 474 1.82e-25 110
Msa0062850 AT5G10250.2 26.392 485 308 16 6 464 53 514 3.26e-25 109
Msa0062850 AT1G67900.5 24.178 517 290 16 6 456 29 509 4.47e-25 109
Msa0062850 AT1G67900.1 24.178 517 290 16 6 456 29 509 4.47e-25 109
Msa0062850 AT1G67900.3 24.178 517 290 16 6 456 29 509 4.47e-25 109
Msa0062850 AT1G67900.6 24.178 517 290 16 6 456 29 509 4.47e-25 109
Msa0062850 AT1G67900.2 24.178 517 290 16 6 456 29 509 4.47e-25 109
Msa0062850 AT1G67900.4 24.178 517 290 16 6 456 29 509 4.47e-25 109
Msa0062850 AT2G30520.1 26.424 439 233 13 14 415 40 425 1.12e-24 108
Msa0062850 AT2G23050.1 25.659 417 250 12 44 451 76 441 2.22e-24 106
Msa0062850 AT4G31820.1 25.635 433 255 18 5 411 29 420 2.27e-24 107
Msa0062850 AT3G49970.1 23.913 506 285 18 17 483 7 451 5.28e-24 105
Msa0062850 AT3G49970.2 23.913 506 285 18 17 483 7 451 5.28e-24 105
Msa0062850 AT5G10250.1 26.427 473 308 16 6 464 53 499 7.56e-24 105
Msa0062850 AT2G30520.2 26.777 422 219 13 31 415 9 377 8.86e-24 105
Msa0062850 AT2G30520.3 26.777 422 219 13 31 415 9 377 8.86e-24 105
Msa0062850 AT5G67385.4 24.459 462 280 14 28 456 4 429 1.52e-23 104
Msa0062850 AT5G67385.5 24.459 462 280 14 28 456 4 429 1.52e-23 104
Msa0062850 AT5G67385.2 24.459 462 280 14 28 456 4 429 1.52e-23 104
Msa0062850 AT5G67385.3 24.459 462 280 14 28 456 4 429 1.52e-23 104
Msa0062850 AT5G03250.2 23.800 479 264 18 86 495 1 447 1.57e-23 103
Msa0062850 AT4G31820.2 26.154 390 226 15 44 411 18 367 2.03e-23 103
Msa0062850 AT2G14820.2 23.158 475 290 15 17 451 40 479 5.85e-23 102
Msa0062850 AT2G14820.1 23.158 475 290 15 17 451 40 479 5.85e-23 102
Msa0062850 AT3G03510.1 24.390 451 266 12 50 488 4 391 1.02e-19 92.0
Msa0062850 AT1G52770.1 33.544 158 96 3 272 420 111 268 2.97e-18 87.8
Msa0062850 AT1G03010.1 29.570 186 97 5 302 454 316 500 1.02e-15 80.5
Msa0062850 AT1G03010.3 29.570 186 97 5 302 454 315 499 1.06e-15 80.5
Msa0062850 AT1G03010.2 29.570 186 97 5 302 454 289 473 1.07e-15 80.5
Msa0062850 AT3G15570.1 26.804 194 121 6 272 460 116 293 3.83e-15 78.2
Msa0062850 AT2G23050.2 26.210 248 168 6 207 451 90 325 9.24e-14 73.2

Find 74 sgRNAs with CRISPR-Local

Find 133 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAAATAGCTATGGTATTT+TGG 0.166395 1_2:-22598440 Msa0062850:CDS
ATTATTGAGAAGTTTCTTAA+AGG 0.209945 1_2:-22598166 Msa0062850:CDS
TGGAGGTATTCTCTGGTTCT+TGG 0.244287 1_2:+22596620 None:intergenic
AGAAACTTCTCAATAATCTT+TGG 0.264089 1_2:+22598172 None:intergenic
AGAGAATGCAATGGAGAGTT+AGG 0.298590 1_2:-22596436 Msa0062850:CDS
AGAACTTGTCATTCTTATCT+TGG 0.311105 1_2:+22596492 None:intergenic
TTAAGAAGATCTATACATTA+TGG 0.321596 1_2:+22596360 None:intergenic
GCAAACATTCAACAACTTCT+AGG 0.327136 1_2:+22597684 None:intergenic
GAGAAGTTTCTTAAAGGTAT+TGG 0.346347 1_2:-22598160 Msa0062850:CDS
CAAACACAAACATTTCTTGA+TGG 0.351667 1_2:-22598535 Msa0062850:CDS
AGATAAGAATGACAAGTTCT+TGG 0.355695 1_2:-22596489 Msa0062850:CDS
GAATACATACCTCGTGTATA+AGG 0.360979 1_2:+22598909 None:intergenic
TTCTCCGAAGAAAATTGTTC+CGG 0.362858 1_2:-22598281 Msa0062850:CDS
TTCTTGATGGAATCTATGAT+TGG 0.373129 1_2:-22598522 Msa0062850:CDS
GATTTCCCTGGAGGACCATA+TGG 0.375612 1_2:-22598691 Msa0062850:CDS
CAATCCTTGGAGGTATTCTC+TGG 0.380799 1_2:+22596613 None:intergenic
TTTGCAAGGGATTGCTTTGA+TGG 0.383370 1_2:-22597656 Msa0062850:CDS
ACCTCCAAGGATTGCAATGC+TGG 0.392173 1_2:-22596604 Msa0062850:CDS
AAGCAATCCCTTGCAAAATC+AGG 0.395103 1_2:+22597662 None:intergenic
GCAATGGAGAGTTAGGGAAT+TGG 0.398547 1_2:-22596429 Msa0062850:CDS
TAAAGATTGTAGAAATGAAA+TGG 0.401095 1_2:-22597930 Msa0062850:CDS
ACTTCATTATTGACCCTAGT+TGG 0.404424 1_2:+22598245 None:intergenic
ATGGAATCTATGATTGGAAA+TGG 0.424156 1_2:-22598516 Msa0062850:CDS
CTCAAACCACCATACTTTGT+TGG 0.428792 1_2:+22598213 None:intergenic
AAGAACATTGGAAGCATTGA+TGG 0.429738 1_2:+22598624 None:intergenic
TTGTGTATTATTAGCAAAGT+TGG 0.430801 1_2:-22598407 Msa0062850:CDS
CAACAAAGTATGGTGGTTTG+AGG 0.437805 1_2:-22598212 Msa0062850:CDS
TCAAACCACCATACTTTGTT+GGG 0.438745 1_2:+22598214 None:intergenic
TCAAATCCATATGGTCCTCC+AGG 0.443640 1_2:+22598685 None:intergenic
TTGGATGATCTACTAGTTTG+TGG 0.458276 1_2:-22597872 Msa0062850:CDS
GAGATCTCAAGGTTCTGATC+AGG 0.459434 1_2:+22597713 None:intergenic
TTTCATATCAATGATTTCCC+TGG 0.460553 1_2:-22598703 Msa0062850:CDS
TTGCAAGGGATTGCTTTGAT+GGG 0.461233 1_2:-22597655 Msa0062850:CDS
CTCTCCATTGCATTCTCTCA+AGG 0.477243 1_2:+22596441 None:intergenic
AATGAAATGGAGAAATTGAT+TGG 0.478241 1_2:-22597917 Msa0062850:CDS
TTTCTTCGGAGAAGAAACTT+TGG 0.478743 1_2:+22598291 None:intergenic
ATTAGTGAGTATACTAGTCT+TGG 0.480817 1_2:-22598052 Msa0062850:CDS
CTAGGAATTTAGAGATCTCA+AGG 0.493304 1_2:+22597702 None:intergenic
AAAGCTAACTCAAATCCATA+TGG 0.495631 1_2:+22598676 None:intergenic
AGATATTGAAGATGAAGAAG+AGG 0.499263 1_2:+22598354 None:intergenic
AGTGCCAGCATTGCAATCCT+TGG 0.499675 1_2:+22596600 None:intergenic
AACTTCTCAATAATCTTTGG+TGG 0.499996 1_2:+22598175 None:intergenic
GAGAATGCAATGGAGAGTTA+GGG 0.504538 1_2:-22596435 Msa0062850:CDS
GATAAAGAGCACAAGAATGA+AGG 0.505168 1_2:+22598599 None:intergenic
ACTTGTCATTCTTATCTTGG+TGG 0.509052 1_2:+22596495 None:intergenic
TATACATATGCAAATAGCTA+TGG 0.512475 1_2:-22598448 Msa0062850:CDS
TGCAAGGGATTGCTTTGATG+GGG 0.523601 1_2:-22597654 Msa0062850:CDS
CTTGGAGAAACAGCTGCTCA+TGG 0.524694 1_2:-22598034 Msa0062850:CDS
GTTCAGATGACAAAGTTCAA+TGG 0.525733 1_2:-22596383 Msa0062850:CDS
ATATTTCCTCATCATTAATG+TGG 0.530774 1_2:+22598933 None:intergenic
AGTTGGATCTCAGTGCAATC+CGG 0.533486 1_2:+22598262 None:intergenic
AATGTTTGAGAAAGCAACAT+TGG 0.542686 1_2:-22597891 Msa0062850:CDS
GCCAGCATTGCAATCCTTGG+AGG 0.544239 1_2:+22596603 None:intergenic
ACTAGTTTGTGGACATGACA+TGG 0.547450 1_2:-22597861 Msa0062850:CDS
AGAATGAAGGATAAGAACAT+TGG 0.549842 1_2:+22598612 None:intergenic
GATTGCACTGAGATCCAACT+AGG 0.550627 1_2:-22598259 Msa0062850:CDS
GGAATTGGAGAAATTATGCA+AGG 0.572806 1_2:-22596414 Msa0062850:CDS
CACCTCAAGATAGATGTCAA+TGG 0.575131 1_2:+22597622 None:intergenic
GAAATGGAGAAATTGATTGG+TGG 0.586984 1_2:-22597914 Msa0062850:CDS
GCAAATCCACATTAATGATG+AGG 0.595863 1_2:-22598939 Msa0062850:CDS
CATATCAATGATTTCCCTGG+AGG 0.597484 1_2:-22598700 Msa0062850:CDS
AGCCATTGACATCTATCTTG+AGG 0.600004 1_2:-22597624 Msa0062850:intron
TCATCAATTGCATAATCACA+TGG 0.618901 1_2:+22596551 None:intergenic
AAGTTGATATCAAAATATTG+TGG 0.624188 1_2:-22598763 Msa0062850:CDS
TAAACCTTGAGAGAATGCAA+TGG 0.625017 1_2:-22596445 Msa0062850:CDS
TGGTACAAAATGATTTGTGA+AGG 0.625971 1_2:+22596515 None:intergenic
CAAATCCATATGGTCCTCCA+GGG 0.630503 1_2:+22598686 None:intergenic
CTAGTTTGTGGACATGACAT+GGG 0.637079 1_2:-22597860 Msa0062850:CDS
GGTACAAAATGATTTGTGAA+GGG 0.674523 1_2:+22596516 None:intergenic
AGAACCAGAGAATACCTCCA+AGG 0.680980 1_2:-22596617 Msa0062850:CDS
ATTGCACTGAGATCCAACTA+GGG 0.684610 1_2:-22598258 Msa0062850:CDS
GCTCATGGAGTCATAAATGT+TGG 0.685224 1_2:-22598019 Msa0062850:CDS
GGAAATATTCCTTATACACG+AGG 0.713652 1_2:-22598918 Msa0062850:intron
TCAATTGCATAATCACATGG+TGG 0.722848 1_2:+22596554 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTGTTATCATATTCAAAT+TGG - chr1_2:22597824-22597843 Msa0062850:CDS 15.0%
!!! AAATATTAGACTTTTTACAT+AGG - chr1_2:22598365-22598384 Msa0062850:CDS 15.0%
!!! AAGTTGATATCAAAATATTG+TGG - chr1_2:22596566-22596585 Msa0062850:CDS 20.0%
!!! TTGAAATTTTGTTACAACAA+TGG - chr1_2:22596674-22596693 Msa0062850:intron 20.0%
!! ATTATTGAGAAGTTTCTTAA+AGG - chr1_2:22597163-22597182 Msa0062850:intron 20.0%
!! TAAAGATTGTAGAAATGAAA+TGG - chr1_2:22597399-22597418 Msa0062850:intron 20.0%
!!! TTATTATGATGTTAGTTTTG+TGG - chr1_2:22597495-22597514 Msa0062850:intron 20.0%
!! AGAAAGATAATAATGCATAA+AGG + chr1_2:22597762-22597781 None:intergenic 20.0%
!!! GATAGAATTTTAACCATTTA+AGG - chr1_2:22597951-22597970 Msa0062850:CDS 20.0%
!! AAAAAGAAATCATCCTTAAA+TGG + chr1_2:22597967-22597986 None:intergenic 20.0%
!! TTAAATAGCATTGTTCTTTA+AGG - chr1_2:22598156-22598175 Msa0062850:CDS 20.0%
!!! TTTTTACATAGGACAAAATT+TGG - chr1_2:22598376-22598395 Msa0062850:CDS 20.0%
! ATATTTCCTCATCATTAATG+TGG + chr1_2:22596399-22596418 None:intergenic 25.0%
! TGAAGATGAATATATGTCTA+AGG - chr1_2:22596518-22596537 Msa0062850:CDS 25.0%
! TATACATATGCAAATAGCTA+TGG - chr1_2:22596881-22596900 Msa0062850:intron 25.0%
! TTGTGTATTATTAGCAAAGT+TGG - chr1_2:22596922-22596941 Msa0062850:intron 25.0%
! AGAAACTTCTCAATAATCTT+TGG + chr1_2:22597160-22597179 None:intergenic 25.0%
!! CTAAGGATTTTATCAAAGTT+TGG - chr1_2:22597370-22597389 Msa0062850:intron 25.0%
! AATGAAATGGAGAAATTGAT+TGG - chr1_2:22597412-22597431 Msa0062850:intron 25.0%
! GAGAAAATGAAAAAAGTTAG+TGG - chr1_2:22597562-22597581 Msa0062850:intron 25.0%
!! CTTTTCAAACAAATTTGACA+TGG - chr1_2:22598069-22598088 Msa0062850:CDS 25.0%
!! AAGAACAATGCTATTTAACA+TGG + chr1_2:22598154-22598173 None:intergenic 25.0%
!! TCAAAATTAGACTTTTGCTT+TGG - chr1_2:22598342-22598361 Msa0062850:CDS 25.0%
! CACAACAAAAATAGAAGATA+AGG - chr1_2:22598450-22598469 Msa0062850:CDS 25.0%
! AAGATCTTTACAAACTTCAA+AGG + chr1_2:22598688-22598707 None:intergenic 25.0%
TTTCATATCAATGATTTCCC+TGG - chr1_2:22596626-22596645 Msa0062850:CDS 30.0%
AAAGCTAACTCAAATCCATA+TGG + chr1_2:22596656-22596675 None:intergenic 30.0%
AGAATGAAGGATAAGAACAT+TGG + chr1_2:22596720-22596739 None:intergenic 30.0%
CAAACACAAACATTTCTTGA+TGG - chr1_2:22596794-22596813 Msa0062850:intron 30.0%
TTCTTGATGGAATCTATGAT+TGG - chr1_2:22596807-22596826 Msa0062850:intron 30.0%
ATGGAATCTATGATTGGAAA+TGG - chr1_2:22596813-22596832 Msa0062850:intron 30.0%
! TGCAAATAGCTATGGTATTT+TGG - chr1_2:22596889-22596908 Msa0062850:intron 30.0%
AGATATTGAAGATGAAGAAG+AGG + chr1_2:22596978-22596997 None:intergenic 30.0%
!! TTCTTCGCGAAATTACTTTT+AGG + chr1_2:22597004-22597023 None:intergenic 30.0%
AACTTCTCAATAATCTTTGG+TGG + chr1_2:22597157-22597176 None:intergenic 30.0%
! GAGAAGTTTCTTAAAGGTAT+TGG - chr1_2:22597169-22597188 Msa0062850:intron 30.0%
! ATTAGTGAGTATACTAGTCT+TGG - chr1_2:22597277-22597296 Msa0062850:intron 30.0%
! GAGAAGAATTTTTCATGTAG+AGG - chr1_2:22597334-22597353 Msa0062850:intron 30.0%
AAATCCTTAGCACACAAAAA+AGG + chr1_2:22597360-22597379 None:intergenic 30.0%
AATGTTTGAGAAAGCAACAT+TGG - chr1_2:22597438-22597457 Msa0062850:intron 30.0%
!!! ATGATGTTAGTTTTGTGGTA+AGG - chr1_2:22597500-22597519 Msa0062850:intron 30.0%
! TTGAAATCATTGGTTGAGTA+CGG + chr1_2:22597885-22597904 None:intergenic 30.0%
AATAAGAGGGTTGAAATCAT+TGG + chr1_2:22597895-22597914 None:intergenic 30.0%
GCATGTGAATGATAATAAGA+GGG + chr1_2:22597908-22597927 None:intergenic 30.0%
AGCATGTGAATGATAATAAG+AGG + chr1_2:22597909-22597928 None:intergenic 30.0%
! TTCACATGCTATAAACCTTT+TGG - chr1_2:22597919-22597938 Msa0062850:CDS 30.0%
TCTATCATTCTCTATCCAAA+AGG + chr1_2:22597937-22597956 None:intergenic 30.0%
ATTGCATGAAAGATTGTGAT+TGG - chr1_2:22598014-22598033 Msa0062850:CDS 30.0%
TTGCATGAAAGATTGTGATT+GGG - chr1_2:22598015-22598034 Msa0062850:CDS 30.0%
CAAAAATAAGTGCAAAAGGA+GGG + chr1_2:22598119-22598138 None:intergenic 30.0%
ACAAAAATAAGTGCAAAAGG+AGG + chr1_2:22598120-22598139 None:intergenic 30.0%
CACACAAAAATAAGTGCAAA+AGG + chr1_2:22598123-22598142 None:intergenic 30.0%
TGGTTATAACTATTTGACAG+TGG - chr1_2:22598308-22598327 Msa0062850:CDS 30.0%
ATAGTAATGATATTGACACC+TGG + chr1_2:22598514-22598533 None:intergenic 30.0%
AGTCAAAAGCTTATGACATT+TGG + chr1_2:22598549-22598568 None:intergenic 30.0%
! GAGTGACTTTAAGAGAATAT+TGG + chr1_2:22598573-22598592 None:intergenic 30.0%
TCATCAATTGCATAATCACA+TGG + chr1_2:22598781-22598800 None:intergenic 30.0%
! GGTACAAAATGATTTGTGAA+GGG + chr1_2:22598816-22598835 None:intergenic 30.0%
! TGGTACAAAATGATTTGTGA+AGG + chr1_2:22598817-22598836 None:intergenic 30.0%
AGAACTTGTCATTCTTATCT+TGG + chr1_2:22598840-22598859 None:intergenic 30.0%
AGATAAGAATGACAAGTTCT+TGG - chr1_2:22598840-22598859 Msa0062850:intron 30.0%
GCAAATCCACATTAATGATG+AGG - chr1_2:22596390-22596409 Msa0062850:CDS 35.0%
GGAAATATTCCTTATACACG+AGG - chr1_2:22596411-22596430 Msa0062850:CDS 35.0%
GAATACATACCTCGTGTATA+AGG + chr1_2:22596423-22596442 None:intergenic 35.0%
TACACGAGGTATGTATTCTA+AGG - chr1_2:22596425-22596444 Msa0062850:CDS 35.0%
!! AAGAACATTGGAAGCATTGA+TGG + chr1_2:22596708-22596727 None:intergenic 35.0%
! GATAAAGAGCACAAGAATGA+AGG + chr1_2:22596733-22596752 None:intergenic 35.0%
TTTCTTCGGAGAAGAAACTT+TGG + chr1_2:22597041-22597060 None:intergenic 35.0%
TTCTCCGAAGAAAATTGTTC+CGG - chr1_2:22597048-22597067 Msa0062850:intron 35.0%
! CAATCCGGAACAATTTTCTT+CGG + chr1_2:22597055-22597074 None:intergenic 35.0%
ACTTCATTATTGACCCTAGT+TGG + chr1_2:22597087-22597106 None:intergenic 35.0%
! CAAGTTTTCCCAACAAAGTA+TGG - chr1_2:22597107-22597126 Msa0062850:intron 35.0%
TCAAACCACCATACTTTGTT+GGG + chr1_2:22597118-22597137 None:intergenic 35.0%
GAAATGGAGAAATTGATTGG+TGG - chr1_2:22597415-22597434 Msa0062850:intron 35.0%
! TTGGATGATCTACTAGTTTG+TGG - chr1_2:22597457-22597476 Msa0062850:intron 35.0%
CTAGGAATTTAGAGATCTCA+AGG + chr1_2:22597630-22597649 None:intergenic 35.0%
GCAAACATTCAACAACTTCT+AGG + chr1_2:22597648-22597667 None:intergenic 35.0%
! AATGTTTGCCTGATTTTGCA+AGG - chr1_2:22597659-22597678 Msa0062850:CDS 35.0%
! ATGTTTGCCTGATTTTGCAA+GGG - chr1_2:22597660-22597679 Msa0062850:CDS 35.0%
AAGTTATTGAGCTCTCAAAG+AGG + chr1_2:22597857-22597876 None:intergenic 35.0%
TGCATGAAAGATTGTGATTG+GGG - chr1_2:22598016-22598035 Msa0062850:CDS 35.0%
!! CTTTAAGGTTCCATTATGCT+TGG - chr1_2:22598171-22598190 Msa0062850:CDS 35.0%
AATGCCATAACCAAGCATAA+TGG + chr1_2:22598184-22598203 None:intergenic 35.0%
ATAAAATCGTCATGCATGAG+TGG - chr1_2:22598288-22598307 Msa0062850:CDS 35.0%
TCATCCAATGATTGCATTTG+AGG - chr1_2:22598620-22598639 Msa0062850:CDS 35.0%
TCAATTGCATAATCACATGG+TGG + chr1_2:22598778-22598797 None:intergenic 35.0%
ACTTGTCATTCTTATCTTGG+TGG + chr1_2:22598837-22598856 None:intergenic 35.0%
TAAACCTTGAGAGAATGCAA+TGG - chr1_2:22598884-22598903 Msa0062850:intron 35.0%
GGAATTGGAGAAATTATGCA+AGG - chr1_2:22598915-22598934 Msa0062850:intron 35.0%
GTTCAGATGACAAAGTTCAA+TGG - chr1_2:22598946-22598965 Msa0062850:CDS 35.0%
CATATCAATGATTTCCCTGG+AGG - chr1_2:22596629-22596648 Msa0062850:CDS 40.0%
ATTGCACTGAGATCCAACTA+GGG - chr1_2:22597071-22597090 Msa0062850:intron 40.0%
! GTTTTCCCAACAAAGTATGG+TGG - chr1_2:22597110-22597129 Msa0062850:intron 40.0%
CTCAAACCACCATACTTTGT+TGG + chr1_2:22597119-22597138 None:intergenic 40.0%
CAACAAAGTATGGTGGTTTG+AGG - chr1_2:22597117-22597136 Msa0062850:intron 40.0%
GCTCATGGAGTCATAAATGT+TGG - chr1_2:22597310-22597329 Msa0062850:intron 40.0%
! ACTAGTTTGTGGACATGACA+TGG - chr1_2:22597468-22597487 Msa0062850:intron 40.0%
! CTAGTTTGTGGACATGACAT+GGG - chr1_2:22597469-22597488 Msa0062850:intron 40.0%
AAGCAATCCCTTGCAAAATC+AGG + chr1_2:22597670-22597689 None:intergenic 40.0%
TTTGCAAGGGATTGCTTTGA+TGG - chr1_2:22597673-22597692 Msa0062850:CDS 40.0%
TTGCAAGGGATTGCTTTGAT+GGG - chr1_2:22597674-22597693 Msa0062850:CDS 40.0%
AGCCATTGACATCTATCTTG+AGG - chr1_2:22597705-22597724 Msa0062850:CDS 40.0%
CACCTCAAGATAGATGTCAA+TGG + chr1_2:22597710-22597729 None:intergenic 40.0%
! GATTGTGATTGGGGAAGTAT+AGG - chr1_2:22598025-22598044 Msa0062850:CDS 40.0%
GACATGGTACATACCATGTA+TGG - chr1_2:22598085-22598104 Msa0062850:CDS 40.0%
GGTTCCATTATGCTTGGTTA+TGG - chr1_2:22598177-22598196 Msa0062850:CDS 40.0%
TGCTTGGTTATGGCATTATG+TGG - chr1_2:22598187-22598206 Msa0062850:CDS 40.0%
GCTTCATTACCAACTAGACA+GGG + chr1_2:22598483-22598502 None:intergenic 40.0%
AGCTTCATTACCAACTAGAC+AGG + chr1_2:22598484-22598503 None:intergenic 40.0%
GTAATGAAGCTTTACTTGCC+AGG - chr1_2:22598493-22598512 Msa0062850:CDS 40.0%
CTCTCCTCAAATGCAATCAT+TGG + chr1_2:22598627-22598646 None:intergenic 40.0%
AGAGAATGCAATGGAGAGTT+AGG - chr1_2:22598893-22598912 Msa0062850:intron 40.0%
GAGAATGCAATGGAGAGTTA+GGG - chr1_2:22598894-22598913 Msa0062850:intron 40.0%
CAAATCCATATGGTCCTCCA+GGG + chr1_2:22596646-22596665 None:intergenic 45.0%
TCAAATCCATATGGTCCTCC+AGG + chr1_2:22596647-22596666 None:intergenic 45.0%
AGTTGGATCTCAGTGCAATC+CGG + chr1_2:22597070-22597089 None:intergenic 45.0%
GATTGCACTGAGATCCAACT+AGG - chr1_2:22597070-22597089 Msa0062850:intron 45.0%
!!! GAGGCCTTTTTTGTGTGCTA+AGG - chr1_2:22597353-22597372 Msa0062850:intron 45.0%
! GAGATCTCAAGGTTCTGATC+AGG + chr1_2:22597619-22597638 None:intergenic 45.0%
TGCAAGGGATTGCTTTGATG+GGG - chr1_2:22597675-22597694 Msa0062850:CDS 45.0%
GAGGGATGTAACACCATACA+TGG + chr1_2:22598101-22598120 None:intergenic 45.0%
GGTAAATTGCCCTGTCTAGT+TGG - chr1_2:22598471-22598490 Msa0062850:CDS 45.0%
GGATGAGACTGCAGATTCAT+TGG + chr1_2:22598606-22598625 None:intergenic 45.0%
!! TGGAGGTATTCTCTGGTTCT+TGG + chr1_2:22598712-22598731 None:intergenic 45.0%
AGAACCAGAGAATACCTCCA+AGG - chr1_2:22598712-22598731 Msa0062850:CDS 45.0%
!! CAATCCTTGGAGGTATTCTC+TGG + chr1_2:22598719-22598738 None:intergenic 45.0%
CTCTCCATTGCATTCTCTCA+AGG + chr1_2:22598891-22598910 None:intergenic 45.0%
GCAATGGAGAGTTAGGGAAT+TGG - chr1_2:22598900-22598919 Msa0062850:intron 45.0%
GATTTCCCTGGAGGACCATA+TGG - chr1_2:22596638-22596657 Msa0062850:intron 50.0%
CTTGGAGAAACAGCTGCTCA+TGG - chr1_2:22597295-22597314 Msa0062850:intron 50.0%
ACCTCCAAGGATTGCAATGC+TGG - chr1_2:22598725-22598744 Msa0062850:CDS 50.0%
AGTGCCAGCATTGCAATCCT+TGG + chr1_2:22598732-22598751 None:intergenic 50.0%
GCCAGCATTGCAATCCTTGG+AGG + chr1_2:22598729-22598748 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 22596370 22598981 22596370 ID=Msa0062850;Name=Msa0062850
chr1_2 mRNA 22596370 22598981 22596370 ID=Msa0062850-mRNA-1;Parent=Msa0062850;Name=Msa0062850-mRNA-1;_AED=0.06;_eAED=0.09;_QI=0|0|0|1|1|1|3|0|500
chr1_2 exon 22598919 22598981 22598919 ID=Msa0062850-mRNA-1:exon:8337;Parent=Msa0062850-mRNA-1
chr1_2 exon 22597625 22598785 22597625 ID=Msa0062850-mRNA-1:exon:8336;Parent=Msa0062850-mRNA-1
chr1_2 exon 22596370 22596648 22596370 ID=Msa0062850-mRNA-1:exon:8335;Parent=Msa0062850-mRNA-1
chr1_2 CDS 22598919 22598981 22598919 ID=Msa0062850-mRNA-1:cds;Parent=Msa0062850-mRNA-1
chr1_2 CDS 22597625 22598785 22597625 ID=Msa0062850-mRNA-1:cds;Parent=Msa0062850-mRNA-1
chr1_2 CDS 22596370 22596648 22596370 ID=Msa0062850-mRNA-1:cds;Parent=Msa0062850-mRNA-1
Gene Sequence

>Msa0062850

ATGTCAAAATTCTGTGACATGCAAATCCACATTAATGATGAGGAAATATTCCTTATACACGAGAAGTTGATATCAAAATATTGTGGAAAATTGAAGAAAATGTTAAATGAAGAAAAGAGAATATTTCATATCAATGATTTCCCTGGAGGACCATATGGATTTGAGTTAGCTTTGAAATTTTGTTACAACAATGGCAAAATCACCATCAATGCTTCCAATGTTCTTATCCTTCATTCTTGTGCTCTTTATCTTGAAATGAGTGAAGAAGTTTCTAGCAACAATCTCTTACAACAAACACAAACATTTCTTGATGGAATCTATGATTGGAAATGGAATGAAATAATAGCAAGTCTCAAGTGTTGTGAATGTGAGAAATTTTATACATATGCAAATAGCTATGGTATTTTGGAGAAGATTATTTGTGTATTATTAGCAAAGTTGGTTCAAAATTCTGATTTCAATTTCATCACTTCCTCTTCTTCATCTTCAATATCTTCTCCTAAAAGTAATTTCGCGAAGAAACTCTCTTTTTCGACCAAAGTTTCTTCTCCGAAGAAAATTGTTCCGGATTGCACTGAGATCCAACTAGGGTCAATAATGAAGTCAAGTTTTCCCAACAAAGTATGGTGGTTTGAGGATTTGTCTACTTTACCACCAAAGATTATTGAGAAGTTTCTTAAAGGTATTGGAGCTTATAAAAGTGATAACAAAAATTTAATTGTCACAAGATTTCTACTTTATTATATGAAAAAAATTACTTCACATTGCAAAAGTATTAGTGAGTATACTAGTCTTGGAGAAACAGCTGCTCATGGAGTCATAAATGTTGGTGAGAAGAATTTTTCATGTAGAGGCCTTTTTTGTGTGCTAAGGATTTTATCAAAGTTTGGCATAAGTAAAGATTGTAGAAATGAAATGGAGAAATTGATTGGTGGAATGTTTGAGAAAGCAACATTGGATGATCTACTAGTTTGTGGACATGACATGGGACTTTATTATGATGTTAGTTTTGTGGTAAGGTTGATTAAAGTATTTGTTAGTATCAATGAAAATGATGTAGAGAAAATGAAAAAAGTTAGTGGATTAATTGACAAGTATTTGATAGAGATATCTCCTGATCAGAACCTTGAGATCTCTAAATTCCTAGAAGTTGTTGAATGTTTGCCTGATTTTGCAAGGGATTGCTTTGATGGGGTCTATAGAGCCATTGACATCTATCTTGAGATCTTGCCAAGAACCAGAGAATACCTCCAAGGATTGCAATGCTGGCACTTATGTCTCGACAAAAAAAATGTTCCACCATGTGATTATGCAATTGATGAAAGTGAAATGAGCCCTTCACAAATCATTTTGTACCACCAAGATAAGAATGACAAGTTCTTGGAAGATAAAGAAGATATGAGAATAAACCTTGAGAGAATGCAATGGAGAGTTAGGGAATTGGAGAAATTATGCAAGGAAATGAAAGTTCAGATGACAAAGTTCAATGGCCATAATGTATAG
Protein sequence

>Msa0062850

MSKFCDMQIHINDEEIFLIHEKLISKYCGKLKKMLNEEKRIFHINDFPGGPYGFELALKFCYNNGKITINASNVLILHSCALYLEMSEEVSSNNLLQQTQTFLDGIYDWKWNEIIASLKCCECEKFYTYANSYGILEKIICVLLAKLVQNSDFNFITSSSSSSISSPKSNFAKKLSFSTKVSSPKKIVPDCTEIQLGSIMKSSFPNKVWWFEDLSTLPPKIIEKFLKGIGAYKSDNKNLIVTRFLLYYMKKITSHCKSISEYTSLGETAAHGVINVGEKNFSCRGLFCVLRILSKFGISKDCRNEMEKLIGGMFEKATLDDLLVCGHDMGLYYDVSFVVRLIKVFVSINENDVEKMKKVSGLIDKYLIEISPDQNLEISKFLEVVECLPDFARDCFDGVYRAIDIYLEILPRTREYLQGLQCWHLCLDKKNVPPCDYAIDESEMSPSQIILYHQDKNDKFLEDKEDMRINLERMQWRVRELEKLCKEMKVQMTKFNGHNV*