Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0064980 | KEH15532.1 | 74.194 | 62 | 10 | 1 | 1 | 56 | 247 | 308 | 1.38e-18 | 87.4 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0064980 | A0A072TDQ4 | 74.194 | 62 | 10 | 1 | 1 | 56 | 247 | 308 | 6.61e-19 | 87.4 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0064970 | Msa0064980 | 0.814430 | 1.546941e-51 | -8.615850e-47 |
| Msa0064980 | Msa0142220 | 0.800911 | 1.154063e-48 | -8.615850e-47 |
| Msa0064980 | Msa0761470 | 0.802629 | 5.124297e-49 | -8.615850e-47 |
| Msa0064980 | Msa1284680 | 0.806228 | 9.109850e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 8 sgRNAs with CRISPR-Local
Find 11 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTTGAGATAAAGAGGAAAA+TGG | 0.370037 | 1_2:+26460636 | Msa0064980:CDS |
| ACTAAAAGATTATGAACTAG+AGG | 0.493307 | 1_2:+26460759 | Msa0064980:CDS |
| ATACATCAAATATGAATCCA+AGG | 0.538148 | 1_2:+26460734 | Msa0064980:CDS |
| GATGCAGAGCTGTAGAAGGC+TGG | 0.573955 | 1_2:+26460661 | Msa0064980:intron |
| CTAAAAGATTATGAACTAGA+GGG | 0.605139 | 1_2:+26460760 | Msa0064980:CDS |
| TACATCAAATATGAATCCAA+GGG | 0.613150 | 1_2:+26460735 | Msa0064980:CDS |
| TGATTTAGAAATACTCTCGA+AGG | 0.636930 | 1_2:+26460711 | Msa0064980:CDS |
| AGGCTGGTAATGATAGCGAA+AGG | 0.640851 | 1_2:+26460677 | Msa0064980:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATACATCAAATATGAATCCA+AGG | + | chr1_2:26460734-26460753 | Msa0064980:CDS | 25.0% |
| ! | TACATCAAATATGAATCCAA+GGG | + | chr1_2:26460735-26460754 | Msa0064980:CDS | 25.0% |
| ! | ACTAAAAGATTATGAACTAG+AGG | + | chr1_2:26460759-26460778 | Msa0064980:CDS | 25.0% |
| ! | CTAAAAGATTATGAACTAGA+GGG | + | chr1_2:26460760-26460779 | Msa0064980:CDS | 25.0% |
| GTGAAAAAGTTGAGATAAAG+AGG | + | chr1_2:26460629-26460648 | Msa0064980:CDS | 30.0% | |
| AGTTGAGATAAAGAGGAAAA+TGG | + | chr1_2:26460636-26460655 | Msa0064980:CDS | 30.0% | |
| TGATTTAGAAATACTCTCGA+AGG | + | chr1_2:26460711-26460730 | Msa0064980:CDS | 30.0% | |
| !!! | TCATAATCTTTTAGTTCCCT+TGG | - | chr1_2:26460754-26460773 | None:intergenic | 30.0% |
| ! | AGGCTGGTAATGATAGCGAA+AGG | + | chr1_2:26460677-26460696 | Msa0064980:CDS | 45.0% |
| ! | GGTTGATGCAGAGCTGTAGA+AGG | + | chr1_2:26460657-26460676 | Msa0064980:intron | 50.0% |
| GATGCAGAGCTGTAGAAGGC+TGG | + | chr1_2:26460661-26460680 | Msa0064980:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_2 | gene | 26460592 | 26460798 | 26460592 | ID=Msa0064980;Name=Msa0064980 |
| chr1_2 | mRNA | 26460592 | 26460798 | 26460592 | ID=Msa0064980-mRNA-1;Parent=Msa0064980;Name=Msa0064980-mRNA-1;_AED=0.48;_eAED=0.48;_QI=0|0|0|1|0|0|2|0|62 |
| chr1_2 | exon | 26460592 | 26460657 | 26460592 | ID=Msa0064980-mRNA-1:exon:9389;Parent=Msa0064980-mRNA-1 |
| chr1_2 | exon | 26460676 | 26460798 | 26460676 | ID=Msa0064980-mRNA-1:exon:9390;Parent=Msa0064980-mRNA-1 |
| chr1_2 | CDS | 26460592 | 26460657 | 26460592 | ID=Msa0064980-mRNA-1:cds;Parent=Msa0064980-mRNA-1 |
| chr1_2 | CDS | 26460676 | 26460798 | 26460676 | ID=Msa0064980-mRNA-1:cds;Parent=Msa0064980-mRNA-1 |
| Gene Sequence |
| Protein sequence |
