AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0066560

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0066560 MtrunA17_Chr1g0170211 98.864 88 1 0 38 125 194 281 7.45e-58 182
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0066560 AT2G26230.1 65.909 88 30 0 32 119 188 275 2.59e-37 130

Find 29 sgRNAs with CRISPR-Local

Find 218 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTAAAATAAAGCTGCTTCT+TGG 0.267652 1_2:-30442965 None:intergenic
GTAAAATAAAGCTGCTTCTT+GGG 0.267918 1_2:-30442964 None:intergenic
GCTGGATTGGGGCTAGAGTT+TGG 0.337886 1_2:-30440274 None:intergenic
TCGTATGAAGAAGCTGGATT+GGG 0.384461 1_2:-30440286 None:intergenic
GGGCTATAGACCCCTTCCTT+TGG 0.385559 1_2:-30443039 None:intergenic
TGGCAAAGGCAGCACTAAAC+AGG 0.427641 1_2:+30443088 Msa0066560:CDS
TTAGTGCTGCCTTTGCCATC+TGG 0.429701 1_2:-30443083 None:intergenic
ACTGGTATGAAATGAATGTT+TGG 0.444035 1_2:-30443662 None:intergenic
TCTTCATACGAGGAAGTTGA+TGG 0.446166 1_2:+30440298 Msa0066560:CDS
CTCGTATGAAGAAGCTGGAT+TGG 0.451969 1_2:-30440287 None:intergenic
CTGCCTTTGCCATCTGGTAG+AGG 0.460839 1_2:-30443077 None:intergenic
TTCTTGTTTGAGAGATTGAC+TGG 0.487296 1_2:-30443680 None:intergenic
TAGAGGGTGGCTTGAACAGA+TGG 0.490129 1_2:-30443060 None:intergenic
AATATTACAAACTCAAGAAA+AGG 0.496524 1_2:-30443793 None:intergenic
GTCAATCTCTCAAACAAGAA+TGG 0.502485 1_2:+30443683 Msa0066560:CDS
ACTTCCTCGTATGAAGAAGC+TGG 0.516760 1_2:-30440292 None:intergenic
AGTTGAATAGAAGCTATGTC+AGG 0.516934 1_2:-30443632 None:intergenic
TGCCTTTGCCATCTGGTAGA+GGG 0.517229 1_2:-30443076 None:intergenic
TGGTTGTATGAAAACATAAC+GGG 0.519658 1_2:-30443762 None:intergenic
CAAGAATGGTCAGTTTGTAA+AGG 0.521979 1_2:+30443697 Msa0066560:CDS
GTGGTTGTATGAAAACATAA+CGG 0.560062 1_2:-30443763 None:intergenic
TAAAATAAAGCTGCTTCTTG+GGG 0.561360 1_2:-30442963 None:intergenic
CTTTGCCATCTGGTAGAGGG+TGG 0.588349 1_2:-30443073 None:intergenic
CTCAAGAAAAGGTGATGCCG+TGG 0.604704 1_2:-30443782 None:intergenic
AGAGGGTGGCTTGAACAGAT+GGG 0.615579 1_2:-30443059 None:intergenic
CGTATGAAGAAGCTGGATTG+GGG 0.620309 1_2:-30440285 None:intergenic
CAATCCAGCTTCTTCATACG+AGG 0.625422 1_2:+30440288 Msa0066560:CDS
TCAAGCCACCCTCTACCAGA+TGG 0.647355 1_2:+30443068 Msa0066560:CDS
CACCCTCTACCAGATGGCAA+AGG 0.652477 1_2:+30443074 Msa0066560:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATGTTATGGAAATTTA+AGG + chr1_2:30441435-30441454 Msa0066560:intron 15.0%
!! ATGACATATTATGAAATATT+TGG + chr1_2:30441602-30441621 Msa0066560:intron 15.0%
!! ATTTAGAAATAATTCAAGTA+CGG + chr1_2:30441704-30441723 Msa0066560:intron 15.0%
!! ATATAATTATCAGAATATCA+AGG + chr1_2:30441914-30441933 Msa0066560:intron 15.0%
!! TATAATTATCAGAATATCAA+GGG + chr1_2:30441915-30441934 Msa0066560:intron 15.0%
!! ATTACGTATAAGAAATTAAT+TGG + chr1_2:30441958-30441977 Msa0066560:intron 15.0%
!!! TTAGTTATTTCATTTTGGTT+TGG - chr1_2:30440395-30440414 None:intergenic 20.0%
!!! TCTTCTTAGTTATTTCATTT+TGG - chr1_2:30440400-30440419 None:intergenic 20.0%
!!! ATATTATTTTAGAAATCACC+AGG - chr1_2:30440537-30440556 None:intergenic 20.0%
!!! AACAGTATTTTACAGATTTA+TGG - chr1_2:30440969-30440988 None:intergenic 20.0%
!! ATAAATCTGTAAAATACTGT+TGG + chr1_2:30440968-30440987 Msa0066560:intron 20.0%
!! TAAATCTGTAAAATACTGTT+GGG + chr1_2:30440969-30440988 Msa0066560:intron 20.0%
!! CTTAAACTTGAAAAGTTTAA+TGG - chr1_2:30441161-30441180 None:intergenic 20.0%
!! TTAAGTTAGAAATGTTTGAT+AGG + chr1_2:30441274-30441293 Msa0066560:intron 20.0%
!! TAAGTTAGAAATGTTTGATA+GGG + chr1_2:30441275-30441294 Msa0066560:intron 20.0%
!! AAGAACATATGACTTATTTA+TGG + chr1_2:30441321-30441340 Msa0066560:intron 20.0%
!! ACTTATTTATGGTTAATTGA+TGG + chr1_2:30441332-30441351 Msa0066560:intron 20.0%
!! TCTTTCATGAATATATGTTA+TGG + chr1_2:30441425-30441444 Msa0066560:intron 20.0%
!! ATGTTCAAATTAACATCAAA+TGG - chr1_2:30441561-30441580 None:intergenic 20.0%
!!! TTTAATTGGTTTGAAAATGA+TGG + chr1_2:30441764-30441783 Msa0066560:intron 20.0%
!! ATAAAAGACAAGAAAGTAAA+AGG + chr1_2:30442110-30442129 Msa0066560:intron 20.0%
!!! GATTTTGAATGAATTGATTT+CGG - chr1_2:30442602-30442621 None:intergenic 20.0%
!!! TTTTCTGTTCATTCATTATT+TGG + chr1_2:30442811-30442830 Msa0066560:intron 20.0%
! TATTTCTTAAGAGAAATACC+TGG + chr1_2:30440516-30440535 Msa0066560:intron 25.0%
! AATTATAAACTTTGCCAAAG+AGG + chr1_2:30440682-30440701 Msa0066560:intron 25.0%
! ATAAACTTGAATCAATGCTA+AGG - chr1_2:30440719-30440738 None:intergenic 25.0%
!!! TTTGACTTGAATCAATGTTA+TGG - chr1_2:30440778-30440797 None:intergenic 25.0%
!! GATTTAGAGTAAGAGAATAA+GGG - chr1_2:30440917-30440936 None:intergenic 25.0%
!! TGATTTAGAGTAAGAGAATA+AGG - chr1_2:30440918-30440937 None:intergenic 25.0%
! AATTGTATATTGGATGCAAT+AGG - chr1_2:30441068-30441087 None:intergenic 25.0%
! TGCATCCAATATACAATTAT+TGG + chr1_2:30441070-30441089 Msa0066560:intron 25.0%
! ATCCAATATACAATTATTGG+TGG + chr1_2:30441073-30441092 Msa0066560:intron 25.0%
! ATTGAACCTAAATGTTAATG+TGG + chr1_2:30441102-30441121 Msa0066560:intron 25.0%
! TGTTAAATATCCTGATTGTT+GGG - chr1_2:30441198-30441217 None:intergenic 25.0%
! TTGTTAAATATCCTGATTGT+TGG - chr1_2:30441199-30441218 None:intergenic 25.0%
! AAGTTAGAAATGTTTGATAG+GGG + chr1_2:30441276-30441295 Msa0066560:intron 25.0%
!!! ATTGATGGTTGTAGTTTTTT+TGG + chr1_2:30441347-30441366 Msa0066560:intron 25.0%
!!! TTGATGGTTGTAGTTTTTTT+GGG + chr1_2:30441348-30441367 Msa0066560:intron 25.0%
!! AATTTGTTTATCGTTCACTA+TGG - chr1_2:30441405-30441424 None:intergenic 25.0%
! GTTTGTGTTAAATTTACATG+TGG + chr1_2:30441478-30441497 Msa0066560:intron 25.0%
! ACGGAATTAGATAAGTATAT+TGG + chr1_2:30441723-30441742 Msa0066560:intron 25.0%
!!! ATATGTGACTCTGTTTTAAT+TGG + chr1_2:30441750-30441769 Msa0066560:intron 25.0%
! TAGAATAAGGACTATACTAT+TGG - chr1_2:30442207-30442226 None:intergenic 25.0%
! TTGCTGATAAACTTAGAATA+AGG - chr1_2:30442220-30442239 None:intergenic 25.0%
! ATGTTAAAATCAATACGAGT+AGG - chr1_2:30442272-30442291 None:intergenic 25.0%
!!! ACTCGTATTGATTTTAACAT+AGG + chr1_2:30442272-30442291 Msa0066560:intron 25.0%
! TTTAAGTCTGATAATCACAA+GGG - chr1_2:30442346-30442365 None:intergenic 25.0%
! ACAAAAAGTAAGAACTAAGT+TGG + chr1_2:30442675-30442694 Msa0066560:intron 25.0%
! TCATTCTTCTAAATTGAATG+TGG + chr1_2:30442907-30442926 Msa0066560:intron 25.0%
!!! TTATTTTACAGAAAAGTACC+TGG + chr1_2:30442978-30442997 Msa0066560:CDS 25.0%
! TTGTCTCTGATGAATAAAAA+AGG + chr1_2:30443159-30443178 Msa0066560:intron 25.0%
! TGTCTCTGATGAATAAAAAA+GGG + chr1_2:30443160-30443179 Msa0066560:intron 25.0%
! ATATGATATCCCTGTTTAAA+TGG + chr1_2:30443483-30443502 Msa0066560:intron 25.0%
AAGTGATGCATTAGAGAAAA+AGG + chr1_2:30440367-30440386 Msa0066560:intron 30.0%
AAATACCTGATGAAGAGATA+TGG + chr1_2:30440473-30440492 Msa0066560:intron 30.0%
TCTCTTAAGAAATAACATCC+AGG - chr1_2:30440510-30440529 None:intergenic 30.0%
TAAGAGTTGTAACTCTTCAT+GGG + chr1_2:30440587-30440606 Msa0066560:intron 30.0%
TGGGAATCATTCATATACAT+TGG + chr1_2:30440606-30440625 Msa0066560:intron 30.0%
TACATTGGAATCTTTGACAA+AGG + chr1_2:30440621-30440640 Msa0066560:intron 30.0%
ACATTGGAATCTTTGACAAA+GGG + chr1_2:30440622-30440641 Msa0066560:intron 30.0%
ATACACTTGAATCAATGCTA+AGG - chr1_2:30440660-30440679 None:intergenic 30.0%
GTTTATATGATCATAACCTG+TGG + chr1_2:30440733-30440752 Msa0066560:intron 30.0%
! ATTAAACATCATTTTGCCAC+AGG - chr1_2:30440752-30440771 None:intergenic 30.0%
CTCCACCAATAATTGTATAT+TGG - chr1_2:30441078-30441097 None:intergenic 30.0%
CAGGATATTTAACAATCTTC+AGG + chr1_2:30441204-30441223 Msa0066560:intron 30.0%
TTATCGTTCACTATGGTAAA+TGG - chr1_2:30441398-30441417 None:intergenic 30.0%
GGTTGTAGATAATATATGCA+TGG + chr1_2:30441499-30441518 Msa0066560:intron 30.0%
TGGATGATAAATATGACACT+AGG + chr1_2:30441784-30441803 Msa0066560:intron 30.0%
!! TAATTGGTGTTCTGAAATTC+TGG + chr1_2:30441974-30441993 Msa0066560:intron 30.0%
GTTTAAGTCTGATAATCACA+AGG - chr1_2:30442347-30442366 None:intergenic 30.0%
TATCATCTTGATACATGCTA+AGG - chr1_2:30442465-30442484 None:intergenic 30.0%
GTATCAAGATGATACAAGAA+AGG + chr1_2:30442471-30442490 Msa0066560:intron 30.0%
!!! GTGTGTTTTGTTTAGTTCTA+CGG - chr1_2:30442636-30442655 None:intergenic 30.0%
TCTGTTCATTCATTATTTGG+TGG + chr1_2:30442814-30442833 Msa0066560:intron 30.0%
TGTACAATGATTTCCTTAGT+TGG - chr1_2:30442858-30442877 None:intergenic 30.0%
TACATGTGTTCTCTTTAACT+AGG + chr1_2:30442874-30442893 Msa0066560:intron 30.0%
TCTAAATTGAATGTGGTTGT+AGG + chr1_2:30442914-30442933 Msa0066560:intron 30.0%
TAAAATAAAGCTGCTTCTTG+GGG - chr1_2:30442966-30442985 None:intergenic 30.0%
GTAAAATAAAGCTGCTTCTT+GGG - chr1_2:30442967-30442986 None:intergenic 30.0%
TGTAAAATAAAGCTGCTTCT+TGG - chr1_2:30442968-30442987 None:intergenic 30.0%
! GTTTTGCTTGACACATTTTT+TGG + chr1_2:30443012-30443031 Msa0066560:CDS 30.0%
AATGAGAGAGGAAAATAGAA+AGG + chr1_2:30443356-30443375 Msa0066560:intron 30.0%
AGAGGAAAATAGAAAGGATA+TGG + chr1_2:30443362-30443381 Msa0066560:intron 30.0%
GATTAGGATCCATTTAAACA+GGG - chr1_2:30443495-30443514 None:intergenic 30.0%
AGATTAGGATCCATTTAAAC+AGG - chr1_2:30443496-30443515 None:intergenic 30.0%
AGAGACTTCAGAATAAGATT+AGG - chr1_2:30443511-30443530 None:intergenic 30.0%
TAGCATATGCACTAAATTTG+TGG - chr1_2:30443569-30443588 None:intergenic 30.0%
ACTGGTATGAAATGAATGTT+TGG - chr1_2:30443665-30443684 None:intergenic 30.0%
TGGTTGTATGAAAACATAAC+GGG - chr1_2:30443765-30443784 None:intergenic 30.0%
GTGGTTGTATGAAAACATAA+CGG - chr1_2:30443766-30443785 None:intergenic 30.0%
! GTTATGTTTTCATACAACCA+CGG + chr1_2:30443765-30443784 Msa0066560:CDS 30.0%
! CTTAGAGACATTTTGTTGCA+TGG - chr1_2:30440236-30440255 None:intergenic 35.0%
GTTTACCATATCTCTTCATC+AGG - chr1_2:30440481-30440500 None:intergenic 35.0%
GATATGGTAAACAAGAAACC+TGG + chr1_2:30440489-30440508 Msa0066560:intron 35.0%
CTAAGAGTTGTAACTCTTCA+TGG + chr1_2:30440586-30440605 Msa0066560:intron 35.0%
CATTGGAATCTTTGACAAAG+GGG + chr1_2:30440623-30440642 Msa0066560:intron 35.0%
TGTGCTTTCACTCATATGAA+AGG + chr1_2:30440800-30440819 Msa0066560:intron 35.0%
AGCAAGCATTAAAGAGTTGA+TGG - chr1_2:30440942-30440961 None:intergenic 35.0%
ATCAACTCTTTAATGCTTGC+TGG + chr1_2:30440941-30440960 Msa0066560:intron 35.0%
AGGTTGCCACATTAACATTT+AGG - chr1_2:30441111-30441130 None:intergenic 35.0%
! ATCTTCAGGTTCGATTTCTA+TGG + chr1_2:30441218-30441237 Msa0066560:intron 35.0%
!! GAGAGTTTGTTAACCCAATT+CGG + chr1_2:30442137-30442156 Msa0066560:intron 35.0%
ATACTATTGGATTTCCTTCC+TGG - chr1_2:30442194-30442213 None:intergenic 35.0%
GGTTGTAGTTGTTTGTTTGA+AGG - chr1_2:30442391-30442410 None:intergenic 35.0%
! TACACGAAAGCTTTTAAGCA+AGG - chr1_2:30442412-30442431 None:intergenic 35.0%
ACCAAACATATGCTAGAGTT+TGG + chr1_2:30442505-30442524 Msa0066560:intron 35.0%
ACCAAACTCTAGCATATGTT+TGG - chr1_2:30442509-30442528 None:intergenic 35.0%
ATCTGAGTGTAAGAATCCAT+TGG - chr1_2:30442542-30442561 None:intergenic 35.0%
! GACTTTTCCACATACATTAC+AGG - chr1_2:30442704-30442723 None:intergenic 35.0%
ACAATGATTTCCTTAGTTGG+AGG - chr1_2:30442855-30442874 None:intergenic 35.0%
GATCTATTTAGACACACAGA+GGG - chr1_2:30443129-30443148 None:intergenic 35.0%
AGATCTATTTAGACACACAG+AGG - chr1_2:30443130-30443149 None:intergenic 35.0%
ATTCATCAGAGACAATAGAG+AGG - chr1_2:30443154-30443173 None:intergenic 35.0%
ATGAATAAAAAAGGGCAACC+CGG + chr1_2:30443168-30443187 Msa0066560:intron 35.0%
!!! TTACCCTGTTTTTACACAAG+AGG + chr1_2:30443255-30443274 Msa0066560:intron 35.0%
ATCAGAGACAATACACCAAA+TGG - chr1_2:30443335-30443354 None:intergenic 35.0%
! CATATAATAAAGTGTCTCCG+TGG - chr1_2:30443411-30443430 None:intergenic 35.0%
ACTAAATTTGTGGCACTTAG+AGG - chr1_2:30443559-30443578 None:intergenic 35.0%
AGTTGAATAGAAGCTATGTC+AGG - chr1_2:30443635-30443654 None:intergenic 35.0%
! TTCTTGTTTGAGAGATTGAC+TGG - chr1_2:30443683-30443702 None:intergenic 35.0%
GTCAATCTCTCAAACAAGAA+TGG + chr1_2:30443683-30443702 Msa0066560:CDS 35.0%
CAAGAATGGTCAGTTTGTAA+AGG + chr1_2:30443697-30443716 Msa0066560:CDS 35.0%
TCGTATGAAGAAGCTGGATT+GGG - chr1_2:30440289-30440308 None:intergenic 40.0%
TCTTCATACGAGGAAGTTGA+TGG + chr1_2:30440298-30440317 Msa0066560:CDS 40.0%
AGGTTCATTAAGCACCTCTT+TGG - chr1_2:30440699-30440718 None:intergenic 40.0%
TTAGACACACAGACTGAAAC+AGG + chr1_2:30440863-30440882 Msa0066560:intron 40.0%
TGGTTGTGCAATACACATCA+AGG + chr1_2:30441006-30441025 Msa0066560:intron 40.0%
AGGAACTCATAGTATCCTGA+AGG + chr1_2:30441026-30441045 Msa0066560:intron 40.0%
GGAACTCATAGTATCCTGAA+GGG + chr1_2:30441027-30441046 Msa0066560:intron 40.0%
GAACTCATAGTATCCTGAAG+GGG + chr1_2:30441028-30441047 Msa0066560:intron 40.0%
TGTAAAACTCAAAGCGCATG+AGG - chr1_2:30441131-30441150 None:intergenic 40.0%
ATATCCTGATTGTTGGGAAG+AGG - chr1_2:30441192-30441211 None:intergenic 40.0%
! GTTTTTCCCTGACACACATA+TGG - chr1_2:30441814-30441833 None:intergenic 40.0%
CAACATCTGTTATCTGTGTC+TGG - chr1_2:30442038-30442057 None:intergenic 40.0%
! TAGGTCTAGAGTTGTAAACG+AGG - chr1_2:30442246-30442265 None:intergenic 40.0%
TCGTTTACAACTCTAGACCT+AGG + chr1_2:30442245-30442264 Msa0066560:intron 40.0%
AATCAATACGAGTAGGACCT+AGG - chr1_2:30442265-30442284 None:intergenic 40.0%
TGACTGTAGGATAGTGAGAT+GGG - chr1_2:30442316-30442335 None:intergenic 40.0%
TTGACTGTAGGATAGTGAGA+TGG - chr1_2:30442317-30442336 None:intergenic 40.0%
!! TTTGGTGTTTGCTTCTCCAA+TGG + chr1_2:30442523-30442542 Msa0066560:intron 40.0%
TTGGCTTCCTGTAATGTATG+TGG + chr1_2:30442694-30442713 Msa0066560:intron 40.0%
! AATCAACCTCTGGAAGATTG+AGG - chr1_2:30442746-30442765 None:intergenic 40.0%
! CTTCCAGAGGTTGATTCTAT+AGG + chr1_2:30442750-30442769 Msa0066560:intron 40.0%
AAGCCTATAGAATCAACCTC+TGG - chr1_2:30442756-30442775 None:intergenic 40.0%
ATTTCCTTAGTTGGAGGATG+TGG - chr1_2:30442849-30442868 None:intergenic 40.0%
! GCAAAACTCTTTTCACATCC+AGG - chr1_2:30442999-30443018 None:intergenic 40.0%
!!! CACATTTTTTGGCTCTCCAA+AGG + chr1_2:30443023-30443042 Msa0066560:CDS 40.0%
!!! TTTTTTGGCTCTCCAAAGGA+AGG + chr1_2:30443027-30443046 Msa0066560:CDS 40.0%
!!! TTTTTGGCTCTCCAAAGGAA+GGG + chr1_2:30443028-30443047 Msa0066560:CDS 40.0%
TATTTAGACACACAGAGGGT+AGG - chr1_2:30443125-30443144 None:intergenic 40.0%
CGTACAATACACCAAATGGT+GGG - chr1_2:30443230-30443249 None:intergenic 40.0%
CAGCCTCTTGTGTAAAAACA+GGG - chr1_2:30443261-30443280 None:intergenic 40.0%
ACAGCCTCTTGTGTAAAAAC+AGG - chr1_2:30443262-30443281 None:intergenic 40.0%
GTTGCTGTCATATGACTGAA+AGG - chr1_2:30443306-30443325 None:intergenic 40.0%
ACAATACACCAAATGGTCAC+GGG - chr1_2:30443328-30443347 None:intergenic 40.0%
GACAATACACCAAATGGTCA+CGG - chr1_2:30443329-30443348 None:intergenic 40.0%
TTGTCTCTGATGAATGAGAG+AGG + chr1_2:30443344-30443363 Msa0066560:intron 40.0%
! CAACTTCTGCAAGCTTTTCA+GGG - chr1_2:30443736-30443755 None:intergenic 40.0%
! TCAACTTCTGCAAGCTTTTC+AGG - chr1_2:30443737-30443756 None:intergenic 40.0%
CGTATGAAGAAGCTGGATTG+GGG - chr1_2:30440288-30440307 None:intergenic 45.0%
CTCGTATGAAGAAGCTGGAT+TGG - chr1_2:30440290-30440309 None:intergenic 45.0%
CAATCCAGCTTCTTCATACG+AGG + chr1_2:30440288-30440307 Msa0066560:CDS 45.0%
ACTTCCTCGTATGAAGAAGC+TGG - chr1_2:30440295-30440314 None:intergenic 45.0%
TTCTCATTCCTGTCCTGCAA+AGG - chr1_2:30440436-30440455 None:intergenic 45.0%
TGCAGGACAGGAATGAGAAA+AGG + chr1_2:30440437-30440456 Msa0066560:intron 45.0%
AGGACAGGAATGAGAAAAGG+AGG + chr1_2:30440440-30440459 Msa0066560:intron 45.0%
ATACTGTTGGGAGATACGCT+TGG + chr1_2:30440981-30441000 Msa0066560:intron 45.0%
AGGTGAACCATATGTGTGTC+AGG + chr1_2:30441804-30441823 Msa0066560:intron 45.0%
GGTGAACCATATGTGTGTCA+GGG + chr1_2:30441805-30441824 Msa0066560:intron 45.0%
CTGTTATCTGTGTCTGGTGT+TGG - chr1_2:30442032-30442051 None:intergenic 45.0%
TAGGTGAGTTGTGCCGAATT+GGG - chr1_2:30442153-30442172 None:intergenic 45.0%
GGCACAACTCACCTACATTT+GGG + chr1_2:30442158-30442177 Msa0066560:intron 45.0%
GCACAACTCACCTACATTTG+GGG + chr1_2:30442159-30442178 Msa0066560:intron 45.0%
CACAACTCACCTACATTTGG+GGG + chr1_2:30442160-30442179 Msa0066560:intron 45.0%
ATTGGATTTCCTTCCTGGCT+TGG - chr1_2:30442189-30442208 None:intergenic 45.0%
TGAGATGGGATCTCAGATCT+AGG - chr1_2:30442302-30442321 None:intergenic 45.0%
CAAGGGTTGTGATTGACTGT+AGG - chr1_2:30442329-30442348 None:intergenic 45.0%
TTTGCCACATCCTCCAACTA+AGG + chr1_2:30442842-30442861 Msa0066560:intron 45.0%
!!! TTTTGGCTCTCCAAAGGAAG+GGG + chr1_2:30443029-30443048 Msa0066560:CDS 45.0%
GCGTACAATACACCAAATGG+TGG - chr1_2:30443231-30443250 None:intergenic 45.0%
GCTGCGTACAATACACCAAA+TGG - chr1_2:30443234-30443253 None:intergenic 45.0%
! TTACACAAGAGGCTGTTTCC+AGG + chr1_2:30443266-30443285 Msa0066560:intron 45.0%
AAGGTCACAAGTTCAAGTCC+TGG - chr1_2:30443287-30443306 None:intergenic 45.0%
TGTGCGTTTCAACTTCACCA+CGG + chr1_2:30443391-30443410 Msa0066560:intron 45.0%
! TTGCAAGCACTGTCCTTTGC+AGG + chr1_2:30440420-30440439 Msa0066560:intron 50.0%
GTATCCTGAAGGGGATTCAC+TGG + chr1_2:30441037-30441056 Msa0066560:intron 50.0%
ATGACCAGTGAATCCCCTTC+AGG - chr1_2:30441044-30441063 None:intergenic 50.0%
TCAGCCTCTTCCCAACAATC+AGG + chr1_2:30441185-30441204 Msa0066560:intron 50.0%
GTAGGTGAGTTGTGCCGAAT+TGG - chr1_2:30442154-30442173 None:intergenic 50.0%
CGGCACAACTCACCTACATT+TGG + chr1_2:30442157-30442176 Msa0066560:intron 50.0%
CAGACTCCTCAATCTTCCAG+AGG + chr1_2:30442737-30442756 Msa0066560:intron 50.0%
AGAGGGTGGCTTGAACAGAT+GGG - chr1_2:30443062-30443081 None:intergenic 50.0%
TAGAGGGTGGCTTGAACAGA+TGG - chr1_2:30443063-30443082 None:intergenic 50.0%
TGCCTTTGCCATCTGGTAGA+GGG - chr1_2:30443079-30443098 None:intergenic 50.0%
TTAGTGCTGCCTTTGCCATC+TGG - chr1_2:30443086-30443105 None:intergenic 50.0%
TGGCAAAGGCAGCACTAAAC+AGG + chr1_2:30443088-30443107 Msa0066560:CDS 50.0%
CTCAAGAAAAGGTGATGCCG+TGG - chr1_2:30443785-30443804 None:intergenic 50.0%
! GCTGGATTGGGGCTAGAGTT+TGG - chr1_2:30440277-30440296 None:intergenic 55.0%
AGCACTGTCCTTTGCAGGAC+AGG + chr1_2:30440425-30440444 Msa0066560:intron 55.0%
! GTTGGGAGATACGCTTGGTG+TGG + chr1_2:30440986-30441005 Msa0066560:intron 55.0%
! GCTTGGTAGCCCCCAAATGT+AGG - chr1_2:30442172-30442191 None:intergenic 55.0%
GGGCTATAGACCCCTTCCTT+TGG - chr1_2:30443042-30443061 None:intergenic 55.0%
TCAAGCCACCCTCTACCAGA+TGG + chr1_2:30443068-30443087 Msa0066560:CDS 55.0%
CTTTGCCATCTGGTAGAGGG+TGG - chr1_2:30443076-30443095 None:intergenic 55.0%
CACCCTCTACCAGATGGCAA+AGG + chr1_2:30443074-30443093 Msa0066560:CDS 55.0%
CTGCCTTTGCCATCTGGTAG+AGG - chr1_2:30443080-30443099 None:intergenic 55.0%
! GTATGCGGCTTTAGTGCACC+GGG - chr1_2:30443189-30443208 None:intergenic 55.0%
! CGTATGCGGCTTTAGTGCAC+CGG - chr1_2:30443190-30443209 None:intergenic 55.0%
GCACTAAAGCCGCATACGCA+AGG + chr1_2:30443192-30443211 Msa0066560:intron 55.0%
AAAGCCGCATACGCAAGGTC+TGG + chr1_2:30443197-30443216 Msa0066560:intron 55.0%
AAGCCGCATACGCAAGGTCT+GGG + chr1_2:30443198-30443217 Msa0066560:intron 55.0%
CTTCCCAGACCTTGCGTATG+CGG - chr1_2:30443204-30443223 None:intergenic 55.0%
GCATACGCAAGGTCTGGGAA+GGG + chr1_2:30443203-30443222 Msa0066560:intron 55.0%
GACAGCAACCCGTGACCATT+TGG + chr1_2:30443317-30443336 Msa0066560:intron 55.0%
CATTTGGGGGCTACCAAGCC+AGG + chr1_2:30442173-30442192 Msa0066560:intron 60.0%
CGCATACGCAAGGTCTGGGA+AGG + chr1_2:30443202-30443221 Msa0066560:intron 60.0%
CTGGGAAGGGTCCCACCATT+TGG + chr1_2:30443216-30443235 Msa0066560:intron 60.0%
TGGGGGCTACCAAGCCAGGA+AGG + chr1_2:30442177-30442196 Msa0066560:intron 65.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 30440229 30443811 30440229 ID=Msa0066560;Name=Msa0066560
chr1_2 mRNA 30440229 30443811 30440229 ID=Msa0066560-mRNA-1;Parent=Msa0066560;Name=Msa0066560-mRNA-1;_AED=0.66;_eAED=0.66;_QI=0|0|0|0.66|1|1|3|0|155
chr1_2 exon 30440229 30440338 30440229 ID=Msa0066560-mRNA-1:exon:10140;Parent=Msa0066560-mRNA-1
chr1_2 exon 30442936 30443109 30442936 ID=Msa0066560-mRNA-1:exon:10141;Parent=Msa0066560-mRNA-1
chr1_2 exon 30443628 30443811 30443628 ID=Msa0066560-mRNA-1:exon:10142;Parent=Msa0066560-mRNA-1
chr1_2 CDS 30440229 30440338 30440229 ID=Msa0066560-mRNA-1:cds;Parent=Msa0066560-mRNA-1
chr1_2 CDS 30442936 30443109 30442936 ID=Msa0066560-mRNA-1:cds;Parent=Msa0066560-mRNA-1
chr1_2 CDS 30443628 30443811 30443628 ID=Msa0066560-mRNA-1:cds;Parent=Msa0066560-mRNA-1
Gene Sequence

>Msa0066560

ATGTCCATGCAACAAAATGTCTCTAAGCAAGATCTTTTATCAAATCCAAACTCTAGCCCCAATCCAGCTTCTTCATACGAGGAAGTTGATGGTTTGAACAACACTCAAATGTATTCATATGAATCGTTATATAGCGTCCCCAAGAAGCAGCTTTATTTTACAGAAAAGTACCTGGATGTGAAAAGAGTTTTGCTTGACACATTTTTTGGCTCTCCAAAGGAAGGGGTCTATAGCCCATCTGTTCAAGCCACCCTCTACCAGATGGCAAAGGCAGCACTAAACAGATTTCCTGACATAGCTTCTATTCAACTAAAGATGCCAAACATTCATTTCATACCAGTCAATCTCTCAAACAAGAATGGTCAGTTTGTAAAGGTATTGAATCTTTTCCCTGAAAAGCTTGCAGAAGTTGATAGAACCCGTTATGTTTTCATACAACCACGGCATCACCTTTTCTTGAGTTTGTAA
Protein sequence

>Msa0066560

MSMQQNVSKQDLLSNPNSSPNPASSYEEVDGLNNTQMYSYESLYSVPKKQLYFTEKYLDVKRVLLDTFFGSPKEGVYSPSVQATLYQMAKAALNRFPDIASIQLKMPNIHFIPVNLSNKNGQFVKVLNLFPEKLAEVDRTRYVFIQPRHHLFLSL*