AlfalfaGEDB | Msa0013050

M. sativa cultivar zhongmu-4 / Msa0013050

KEGG: -
GO: GO:0000325 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0008150 GO:0009705 GO:0016020 GO:0016032 GO:0019058 GO:0019079 GO:0031090 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046786 GO:0051704 GO:0098588 GO:0098805
PFAM: PF06454.14

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0013050	sp\|Q9ZUM2\|TOM3_ARATH	82.918	281	48	0	17	297	23	303	3.60e-176	491

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000490	Msa0013050	0.830162	3.429550e-55	-8.615850e-47
Msa0004980	Msa0013050	-0.814581	1.432573e-51	-8.615850e-47
Msa0006040	Msa0013050	-0.812862	3.423618e-51	-8.615850e-47
Msa0011960	Msa0013050	-0.816856	4.457682e-52	-8.615850e-47
Msa0012040	Msa0013050	-0.819553	1.093577e-52	-8.615850e-47
Msa0012240	Msa0013050	-0.813703	2.238494e-51	-8.615850e-47
Msa0013050	Msa0017900	0.802712	4.924834e-49	-8.615850e-47
Msa0013050	Msa0023000	-0.834224	3.390926e-56	-8.615850e-47
Msa0013050	Msa0023830	0.823370	1.436743e-53	-8.615850e-47
Msa0013050	Msa0024210	0.842584	2.364816e-58	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0013050	MtrunA17_Chr1g0162201	97.306	297	8	1	297	1	297	0.0	589
Msa0013050	MtrunA17_Chr8g0375261	83.972	287	46	11	297	8	294	0.0	503
Msa0013050	MtrunA17_Chr4g0050391	58.484	277	115	21	297	13	289	7.23e-121	348
Msa0013050	MtrunA17_Chr2g0288191	60.392	255	101	43	297	30	284	2.79e-111	323
Msa0013050	MtrunA17_Chr4g0074691	56.884	276	119	22	297	3	278	2.44e-106	310

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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0013050	AT2G02180.1	82.918	281	48	17	297	23	303	3.66e-177	491
Msa0013050	AT1G14530.2	80.496	282	55	16	297	12	293	8.44e-164	457
Msa0013050	AT1G14530.1	78.855	227	48	16	242	12	238	1.34e-134	381
Msa0013050	AT4G21790.1	59.286	280	114	18	297	12	291	1.31e-123	355

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Find 90 sgRNAs with CRISPR-Local

Find 569 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATGCGAACCTTAATGTGA+TGG	0.567249	1_1:+20198801	Msa0013050:CDS
AACTCACCGATTCCTCAACC+TGG	0.591877	1_1:+20191211	Msa0013050:CDS
AAGATGCGGTCTTGCCAAAC+CGG	0.453837	1_1:-20191255	None:intergenic
AATTTGTAGTTTGCTTGCTA+GGG	0.421160	1_1:-20191044	None:intergenic
ACATCGACGATTCTCCGGTT+TGG	0.476060	1_1:+20191241	Msa0013050:CDS
ACATTAATTGTATAGAAACT+TGG	0.472412	1_1:-20193807	None:intergenic
ACCGCGCCTGTCACCGACGA+CGG	0.629626	1_1:-20191165	None:intergenic
ACCGTAGAGAAGAAAGCCAA+GGG	0.603119	1_1:-20196653	None:intergenic
ACCTCGCCGTCGTCGGTGAC+AGG	0.458409	1_1:+20191159	Msa0013050:CDS
ACTTGGCTTCAGTCCATCAG+TGG	0.611275	1_1:-20193790	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TAAGATTGAATAGGATGAGA+AGG	-	chr1_1:20191330-20191349	None:intergenic	30.0%
TTCTCATCCTATTCAATCTT+AGG	+	chr1_1:20191329-20191348	Msa0013050:intron	30.0%
TCTCATCCTATTCAATCTTA+GGG	+	chr1_1:20191330-20191349	Msa0013050:intron	30.0%
AATTGAGTTACGAATGCATA+GGG	-	chr1_1:20191361-20191380	None:intergenic	30.0%
TAATTGAGTTACGAATGCAT+AGG	-	chr1_1:20191362-20191381	None:intergenic	30.0%
TATATCACATAAAACCCCAT+AGG	-	chr1_1:20191833-20191852	None:intergenic	30.0%
TTTATCGTAGAACCTTAAGA+TGG	+	chr1_1:20191880-20191899	Msa0013050:intron	30.0%
TTATCGTAGAACCTTAAGAT+GGG	+	chr1_1:20191881-20191900	Msa0013050:intron	30.0%
AGCATATCACAAAACATTAG+CGG	-	chr1_1:20192052-20192071	None:intergenic	30.0%
AAAGAGCTTATAGCTTATAG+CGG	+	chr1_1:20192100-20192119	Msa0013050:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_1	gene	20191003	20199345	20191003	ID=Msa0013050;Name=Msa0013050
chr1_1	mRNA	20191003	20199345	20191003	ID=Msa0013050-mRNA-1;Parent=Msa0013050;Name=Msa0013050-mRNA-1;_AED=0.10;_eAED=0.10;_QI=144\|1\|1\|1\|1\|1\|11\|115\|297
chr1_1	exon	20191003	20191323	20191003	ID=Msa0013050-mRNA-1:exon:7194;Parent=Msa0013050-mRNA-1
chr1_1	exon	20191462	20191552	20191462	ID=Msa0013050-mRNA-1:exon:7195;Parent=Msa0013050-mRNA-1
chr1_1	exon	20191674	20191726	20191674	ID=Msa0013050-mRNA-1:exon:7196;Parent=Msa0013050-mRNA-1
chr1_1	exon	20193568	20193660	20193568	ID=Msa0013050-mRNA-1:exon:7197;Parent=Msa0013050-mRNA-1
chr1_1	exon	20193777	20193848	20193777	ID=Msa0013050-mRNA-1:exon:7198;Parent=Msa0013050-mRNA-1
chr1_1	exon	20196464	20196536	20196464	ID=Msa0013050-mRNA-1:exon:7199;Parent=Msa0013050-mRNA-1
chr1_1	exon	20196635	20196680	20196635	ID=Msa0013050-mRNA-1:exon:7200;Parent=Msa0013050-mRNA-1
chr1_1	exon	20197190	20197253	20197190	ID=Msa0013050-mRNA-1:exon:7201;Parent=Msa0013050-mRNA-1
chr1_1	exon	20198641	20198694	20198641	ID=Msa0013050-mRNA-1:exon:7202;Parent=Msa0013050-mRNA-1
chr1_1	exon	20198778	20198855	20198778	ID=Msa0013050-mRNA-1:exon:7203;Parent=Msa0013050-mRNA-1
chr1_1	exon	20199138	20199345	20199138	ID=Msa0013050-mRNA-1:exon:7204;Parent=Msa0013050-mRNA-1
chr1_1	five_prime_UTR	20191003	20191146	20191003	ID=Msa0013050-mRNA-1:five_prime_utr;Parent=Msa0013050-mRNA-1
chr1_1	CDS	20191147	20191323	20191147	ID=Msa0013050-mRNA-1:cds;Parent=Msa0013050-mRNA-1
chr1_1	CDS	20191462	20191552	20191462	ID=Msa0013050-mRNA-1:cds;Parent=Msa0013050-mRNA-1
chr1_1	CDS	20191674	20191726	20191674	ID=Msa0013050-mRNA-1:cds;Parent=Msa0013050-mRNA-1
chr1_1	CDS	20193568	20193660	20193568	ID=Msa0013050-mRNA-1:cds;Parent=Msa0013050-mRNA-1
chr1_1	CDS	20193777	20193848	20193777	ID=Msa0013050-mRNA-1:cds;Parent=Msa0013050-mRNA-1
chr1_1	CDS	20196464	20196536	20196464	ID=Msa0013050-mRNA-1:cds;Parent=Msa0013050-mRNA-1
chr1_1	CDS	20196635	20196680	20196635	ID=Msa0013050-mRNA-1:cds;Parent=Msa0013050-mRNA-1
chr1_1	CDS	20197190	20197253	20197190	ID=Msa0013050-mRNA-1:cds;Parent=Msa0013050-mRNA-1
chr1_1	CDS	20198641	20198694	20198641	ID=Msa0013050-mRNA-1:cds;Parent=Msa0013050-mRNA-1
chr1_1	CDS	20198778	20198855	20198778	ID=Msa0013050-mRNA-1:cds;Parent=Msa0013050-mRNA-1
chr1_1	CDS	20199138	20199230	20199138	ID=Msa0013050-mRNA-1:cds;Parent=Msa0013050-mRNA-1
chr1_1	three_prime_UTR	20199231	20199345	20199231	ID=Msa0013050-mRNA-1:three_prime_utr;Parent=Msa0013050-mRNA-1

Gene Sequence

>Msa0013050

ATGACCAACAACACCTCGCCGTCGTCGGTGACAGGCGCGGTGGTGAACGCGGTGGCAGCAATTGAACTCACCGATTCCTCAACCTGGTGGAACGACATCGACGATTCTCCGGTTTGGCAAGACCGCATCTTCTACACCCTCGCCGGCCTTTACGGCGTCGTTTCCGCCATAGCTTTGATTCAACTTGTTAGAATACAATTGAGAGTTCCAGAATATGGTTGGACTACCCAGAAGGTGTTTCATTTTCTCAATTTTGTGGTGAATGCAGTTCGATGTTTAGTTTTCATCTTCTTTCGGAATGTACAGAGGTTACAGCCAGAGATTGTCCAACATATCTTACTTGATGTGCCAAGTCTTGCTTTCTTTACAACATATGCACTCCTGGTCTTGTTCTGGGCTGAGATTTATTACCAGGCACGTGCTGTATCCACTGATGGACTGAAGCCAAGTTTCTATACAATTAATGTTGTGGTTTATGCTGTTCAGATCATTTTGTGGTTGATATTATGGTGGAAACCAGTCAGCGTACTAGTCATTCTGTCAAAGGTGTTCTTTGCAGCGGTTTCTTTGTTTGCTGCCCTTGGCTTTCTTCTCTACGGTGGAAGACTATTCTTGATGCTGCAACGCTTTCCTGTTGAATCCAAAGGGCGTCGTAAGAAGCTGCAAGAGGTTGGCTATGTGACAACAATATGTTTTCTATGTTTCCTTATCAGAAGTGTTATGATGTGCTTTAATGCATTTGATAAAAATGCGAACCTTAATGTGATGGACCATCCTATTCTAAACTTCATATATTACCTGTTGGTGGAAATATTACCTTCTTCTTTGGTCTTGTTCATTTTGAGGAAGCTTCCACCAAAGCGTGGTATCACACAATATCACCCGATTCGCTGA

Protein sequence

>Msa0013050

MTNNTSPSSVTGAVVNAVAAIELTDSSTWWNDIDDSPVWQDRIFYTLAGLYGVVSAIALIQLVRIQLRVPEYGWTTQKVFHFLNFVVNAVRCLVFIFFRNVQRLQPEIVQHILLDVPSLAFFTTYALLVLFWAEIYYQARAVSTDGLKPSFYTINVVVYAVQIILWLILWWKPVSVLVILSKVFFAAVSLFAALGFLLYGGRLFLMLQRFPVESKGRRKKLQEVGYVTTICFLCFLIRSVMMCFNAFDKNANLNVMDHPILNFIYYLLVEILPSSLVLFILRKLPPKRGITQYHPIR*