AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0069340

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0069340 MtrunA17_Chr1g0158811 97.188 320 9 0 1 320 1 320 0.0 639
Msa0069340 MtrunA17_Chr1g0158821 61.920 323 114 6 1 319 1 318 8.07e-133 380
Msa0069340 MtrunA17_Chr3g0127471 65.000 280 87 5 43 317 36 309 1.67e-120 348
Msa0069340 MtrunA17_Chr5g0419391 47.036 253 131 3 68 317 7 259 3.09e-77 237
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0069340 AT5G24400.1 62.903 248 87 3 72 317 81 325 5.60e-105 310
Msa0069340 AT3G49360.1 54.286 245 103 4 78 317 11 251 1.28e-85 258
Msa0069340 AT1G13700.2 50.000 242 117 2 80 317 20 261 1.16e-81 248
Msa0069340 AT1G13700.3 50.000 242 117 2 80 317 20 261 1.16e-81 248
Msa0069340 AT1G13700.1 50.000 242 117 2 80 317 20 261 1.16e-81 248
Msa0069340 AT5G24410.1 52.653 245 107 4 78 317 17 257 6.53e-80 244
Msa0069340 AT5G24420.1 51.837 245 110 4 78 317 11 252 7.23e-79 241

Find 67 sgRNAs with CRISPR-Local

Find 236 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTGCTTCAAAACTGTTTA+AGG 0.136078 1_2:-37638576 Msa0069340:CDS
ATTCTCTCTGATGGTGGTTT+TGG 0.280005 1_2:+37638773 Msa0069330:three_prime_UTR
TTACTTTCTAAGTTATCTTT+TGG 0.280021 1_2:+37639444 Msa0069330:three_prime_UTR
AACACTTTATTATGCCTTAG+AGG 0.288583 1_2:+37639969 Msa0069330:three_prime_UTR
AAACCATAGATTGGTCAAAA+TGG 0.294312 1_2:-37639497 Msa0069340:CDS
AGGAGCATGAGATCAAATTT+TGG 0.311450 1_2:+37638890 Msa0069330:three_prime_UTR
ATTGATTTCTTTAGTAATAG+TGG 0.312473 1_2:+37640029 Msa0069330:three_prime_UTR
TATTTCACTGTTGCTTTGTC+TGG 0.315803 1_2:-37639795 Msa0069340:CDS
CAATCTATGGTTTCAGCATA+TGG 0.367849 1_2:+37639507 Msa0069330:three_prime_UTR
GTGAACAAAATAAGCTACTT+TGG 0.384187 1_2:+37640063 Msa0069330:three_prime_UTR
TGATCTCATGCTCCTAGGTA+TGG 0.406465 1_2:-37638882 Msa0069340:CDS
TGAAACAACCCTAAGACGTT+TGG 0.412525 1_2:-37638951 Msa0069340:CDS
GGAACATCTTGCTGTGTCAT+TGG 0.418210 1_2:-37639865 Msa0069340:CDS
TTCTCCAAGAGGATCAGAAA+TGG 0.419423 1_2:-37638817 Msa0069340:CDS
AACAAATTCATCAAAGAAAG+AGG 0.421608 1_2:-37639819 Msa0069340:CDS
TTCACTGTTGCTTTGTCTGG+TGG 0.432382 1_2:-37639792 Msa0069340:CDS
GGCATCACTTAAGTGGGAAA+AGG 0.443000 1_2:-37639916 Msa0069340:CDS
TAAACGTTGAGAGGAGGAAT+TGG 0.449024 1_2:+37639019 Msa0069330:three_prime_UTR
TAACCCATTTCTGATCCTCT+TGG 0.454962 1_2:+37638813 Msa0069330:three_prime_UTR
GAAAAGGTTTCACCTCTTGA+AGG 0.456560 1_2:-37638626 Msa0069340:CDS
CATGTTGCATCTTTATTCCC+AGG 0.457125 1_2:-37638848 Msa0069340:CDS
CTCTTGAAGGGGAATTGAAA+TGG 0.460789 1_2:-37638613 Msa0069340:CDS
AAAGATGCAACATGTCCATC+TGG 0.461834 1_2:+37638857 Msa0069330:three_prime_UTR
TAAACATCAGCTGCTCCATC+AGG 0.473530 1_2:+37638974 Msa0069330:three_prime_UTR
AATTATAAGCTTGCCAGTGA+TGG 0.484242 1_2:-37639423 Msa0069340:CDS
GATCTCATGCTCCTAGGTAT+GGG 0.491824 1_2:-37638881 Msa0069340:CDS
TGTCATGTTGCCTGTTGAAA+AGG 0.494449 1_2:-37638642 Msa0069340:CDS
AAATAGAGGCATCACTTAAG+TGG 0.496228 1_2:-37639923 Msa0069340:CDS
CGCAGTTTACACTGCACTTG+AGG 0.500062 1_2:-37638678 Msa0069340:CDS
GCATCACTTAAGTGGGAAAA+GGG 0.504570 1_2:-37639915 Msa0069340:CDS
TCTCCAAGAGGATCAGAAAT+GGG 0.506655 1_2:-37638816 Msa0069340:CDS
CAGCTGCTCCATCAGGTGGT+AGG 0.508616 1_2:+37638981 Msa0069330:three_prime_UTR
AAATTTGATCTCATGCTCCT+AGG 0.509138 1_2:-37638887 Msa0069340:CDS
AAAAGGTTTCACCTCTTGAA+GGG 0.515655 1_2:-37638625 Msa0069340:CDS
AACAAAATAAGCTACTTTGG+AGG 0.518255 1_2:+37640066 Msa0069330:three_prime_UTR
AGTCACCACCATTGCAACAT+TGG 0.519798 1_2:+37638718 Msa0069330:three_prime_UTR
CATATGCTGAAACCATAGAT+TGG 0.520043 1_2:-37639506 Msa0069340:CDS
GATCATTGATCAAGTACCTA+AGG 0.528998 1_2:-37639770 Msa0069340:intron
AGCTGCTCCATCAGGTGGTA+GGG 0.529718 1_2:+37638982 Msa0069330:three_prime_UTR
GTGAAAGTAATTCTCTCTGA+TGG 0.540458 1_2:+37638764 Msa0069330:three_prime_UTR
TTGGAAGAAGCATTGATCAC+TGG 0.541052 1_2:+37638737 Msa0069330:three_prime_UTR
ATTAGTCAAAGTTGTTGAGA+AGG 0.549232 1_2:+37640097 Msa0069330:three_prime_UTR
GCAATGGTGGTGACTGGCGC+GGG 0.551673 1_2:-37638710 Msa0069340:CDS
CTCCTAGGTATGGGTCCAGA+TGG 0.553952 1_2:-37638872 Msa0069340:CDS
AATAGAGGCATCACTTAAGT+GGG 0.554935 1_2:-37639922 Msa0069340:CDS
TTGAAATGGTACTTAGACAA+AGG 0.555841 1_2:-37638599 Msa0069340:CDS
TGCTTCTTCCAATGTTGCAA+TGG 0.565044 1_2:-37638726 Msa0069340:CDS
CATTTCAATTCCCCTTCAAG+AGG 0.566687 1_2:+37638614 Msa0069330:three_prime_UTR
GATGATTCCCTACCACCTGA+TGG 0.566852 1_2:-37638989 Msa0069340:CDS
AATGTTGCAATGGTGGTGAC+TGG 0.569664 1_2:-37638716 Msa0069340:CDS
AGGTGGTAGGGAATCATCAA+TGG 0.569674 1_2:+37638994 Msa0069330:three_prime_UTR
TTCTTCCAATGTTGCAATGG+TGG 0.573190 1_2:-37638723 Msa0069340:CDS
AAAGGGTTATAAGAATGTTG+AGG 0.580239 1_2:-37639898 Msa0069340:CDS
TGCAATGGTGGTGACTGGCG+CGG 0.584998 1_2:-37638711 Msa0069340:CDS
ACAAAATAAGCTACTTTGGA+GGG 0.598728 1_2:+37640067 Msa0069330:three_prime_UTR
AAAGGTTTCACCTCTTGAAG+GGG 0.598919 1_2:-37638624 Msa0069340:CDS
CTTGGAGAGAAATCCATCAC+TGG 0.601315 1_2:+37639410 Msa0069330:three_prime_UTR
CTTGGAGAAGAGCATGACCT+GGG 0.611662 1_2:+37638831 Msa0069330:three_prime_UTR
AAAGTAATTCTCTCTGATGG+TGG 0.620404 1_2:+37638767 Msa0069330:three_prime_UTR
CAGTGATGGATTTCTCTCCA+AGG 0.622114 1_2:-37639409 Msa0069340:intron
TCTTGGAGAAGAGCATGACC+TGG 0.622800 1_2:+37638830 Msa0069330:three_prime_UTR
TCAATGGCATAAACGTTGAG+AGG 0.637656 1_2:+37639010 Msa0069330:three_prime_UTR
ATGGCATAAACGTTGAGAGG+AGG 0.640971 1_2:+37639013 Msa0069330:three_prime_UTR
ACATCAGCTGCTCCATCAGG+TGG 0.642046 1_2:+37638977 Msa0069330:three_prime_UTR
AGGTCATGCTCTTCTCCAAG+AGG 0.654284 1_2:-37638828 Msa0069340:CDS
GTCCATCTGGACCCATACCT+AGG 0.668407 1_2:+37638870 Msa0069330:three_prime_UTR
CTCAAGTGCAGTGTAAACTG+CGG 0.695035 1_2:+37638679 Msa0069330:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAGTAACATTTATATTAAA+AGG + chr1_2:37639370-37639389 Msa0069330:intron 10.0%
!! ATAGTAACATTTATATTAAA+AGG + chr1_2:37639370-37639389 Msa0069330:intron 10.0%
!! TTTGTTAACCTAATTTAAAT+AGG - chr1_2:37638974-37638993 Msa0069340:CDS 15.0%
!!! ATATGATTTTAATGTTACAT+AGG - chr1_2:37639007-37639026 Msa0069340:CDS 15.0%
!!! CAATTTTATGTTTTTATTTC+AGG - chr1_2:37639127-37639146 Msa0069340:intron 15.0%
!! TTTGTTAACCTAATTTAAAT+AGG - chr1_2:37638974-37638993 Msa0069340:CDS 15.0%
!!! ATATGATTTTAATGTTACAT+AGG - chr1_2:37639007-37639026 Msa0069340:CDS 15.0%
!!! CAATTTTATGTTTTTATTTC+AGG - chr1_2:37639127-37639146 Msa0069340:intron 15.0%
!!! ATTGATTTCTTTAGTAATAG+TGG + chr1_2:37638646-37638665 Msa0069330:three_prime_UTR 20.0%
!!! ATAGGTTGGTATAATTTAAA+TGG - chr1_2:37639025-37639044 Msa0069340:CDS 20.0%
!! ATTTAAATGGAATTGTGATT+AGG - chr1_2:37639038-37639057 Msa0069340:intron 20.0%
!!! CACCTTAATTATTTATTTCT+AGG - chr1_2:37639081-37639100 Msa0069340:intron 20.0%
!! AGCCTAGAAATAAATAATTA+AGG + chr1_2:37639086-37639105 Msa0069330:intron 20.0%
!!! ATGTTTTTATTTCAGGAAAT+TGG - chr1_2:37639134-37639153 Msa0069340:intron 20.0%
!!! TACTTTCTAAGTTATCTTTT+GGG + chr1_2:37639230-37639249 Msa0069330:intron 20.0%
!!! TTACTTTCTAAGTTATCTTT+TGG + chr1_2:37639231-37639250 Msa0069330:intron 20.0%
!!! GAAATTAATCATTTTGATGT+TGG - chr1_2:37639343-37639362 Msa0069340:intron 20.0%
!! ATGATATCGTAATTAGATAA+GGG + chr1_2:37639506-37639525 Msa0069330:three_prime_UTR 20.0%
!! TATGATATCGTAATTAGATA+AGG + chr1_2:37639507-37639526 Msa0069330:three_prime_UTR 20.0%
!! TTATCTAATTACGATATCAT+AGG - chr1_2:37639506-37639525 Msa0069340:CDS 20.0%
!!! ATCAAATTTTGGTAATTCAT+TGG + chr1_2:37639774-37639793 Msa0069330:three_prime_UTR 20.0%
!!! ATTGATTTCTTTAGTAATAG+TGG + chr1_2:37638646-37638665 Msa0069330:three_prime_UTR 20.0%
!!! ATAGGTTGGTATAATTTAAA+TGG - chr1_2:37639025-37639044 Msa0069340:CDS 20.0%
!! ATTTAAATGGAATTGTGATT+AGG - chr1_2:37639038-37639057 Msa0069340:intron 20.0%
!!! CACCTTAATTATTTATTTCT+AGG - chr1_2:37639081-37639100 Msa0069340:intron 20.0%
!! AGCCTAGAAATAAATAATTA+AGG + chr1_2:37639086-37639105 Msa0069330:intron 20.0%
!!! ATGTTTTTATTTCAGGAAAT+TGG - chr1_2:37639134-37639153 Msa0069340:intron 20.0%
!!! TACTTTCTAAGTTATCTTTT+GGG + chr1_2:37639230-37639249 Msa0069330:intron 20.0%
!!! TTACTTTCTAAGTTATCTTT+TGG + chr1_2:37639231-37639250 Msa0069330:intron 20.0%
!!! GAAATTAATCATTTTGATGT+TGG - chr1_2:37639343-37639362 Msa0069340:intron 20.0%
!! ATGATATCGTAATTAGATAA+GGG + chr1_2:37639506-37639525 Msa0069330:three_prime_UTR 20.0%
!! TATGATATCGTAATTAGATA+AGG + chr1_2:37639507-37639526 Msa0069330:three_prime_UTR 20.0%
!! TTATCTAATTACGATATCAT+AGG - chr1_2:37639506-37639525 Msa0069340:CDS 20.0%
!!! ATCAAATTTTGGTAATTCAT+TGG + chr1_2:37639774-37639793 Msa0069330:three_prime_UTR 20.0%
!! AACAATGTTTTTCCCAATTT+GGG + chr1_2:37638677-37638696 Msa0069330:three_prime_UTR 25.0%
!! AAACAATGTTTTTCCCAATT+TGG + chr1_2:37638678-37638697 Msa0069330:three_prime_UTR 25.0%
! AACAAATTCATCAAAGAAAG+AGG - chr1_2:37638853-37638872 Msa0069340:CDS 25.0%
! TTAACCTAATTTAAATAGGC+TGG - chr1_2:37638978-37638997 Msa0069340:CDS 25.0%
!!! GATTTTAATGTTACATAGGT+TGG - chr1_2:37639011-37639030 Msa0069340:CDS 25.0%
! AAATCGAACAACTAATCAAA+TGG + chr1_2:37639464-37639483 Msa0069330:three_prime_UTR 25.0%
! GAATTAACAATCATCAAACT+TGG + chr1_2:37639545-37639564 Msa0069330:intron 25.0%
! TCGACAATAAGATTTATTGA+CGG + chr1_2:37639568-37639587 Msa0069330:intron 25.0%
!! AACAATGTTTTTCCCAATTT+GGG + chr1_2:37638677-37638696 Msa0069330:three_prime_UTR 25.0%
!! AAACAATGTTTTTCCCAATT+TGG + chr1_2:37638678-37638697 Msa0069330:three_prime_UTR 25.0%
! AACAAATTCATCAAAGAAAG+AGG - chr1_2:37638853-37638872 Msa0069340:CDS 25.0%
! TTAACCTAATTTAAATAGGC+TGG - chr1_2:37638978-37638997 Msa0069340:CDS 25.0%
!!! GATTTTAATGTTACATAGGT+TGG - chr1_2:37639011-37639030 Msa0069340:CDS 25.0%
! AAATCGAACAACTAATCAAA+TGG + chr1_2:37639464-37639483 Msa0069330:three_prime_UTR 25.0%
! GAATTAACAATCATCAAACT+TGG + chr1_2:37639545-37639564 Msa0069330:intron 25.0%
! TCGACAATAAGATTTATTGA+CGG + chr1_2:37639568-37639587 Msa0069330:intron 25.0%
ATTAGTCAAAGTTGTTGAGA+AGG + chr1_2:37638578-37638597 Msa0069330:three_prime_UTR 30.0%
! ACAAAATAAGCTACTTTGGA+GGG + chr1_2:37638608-37638627 Msa0069330:three_prime_UTR 30.0%
! AACAAAATAAGCTACTTTGG+AGG + chr1_2:37638609-37638628 Msa0069330:three_prime_UTR 30.0%
GTGAACAAAATAAGCTACTT+TGG + chr1_2:37638612-37638631 Msa0069330:three_prime_UTR 30.0%
! TCAATTTTACCAACCCAAAT+TGG - chr1_2:37638661-37638680 Msa0069340:CDS 30.0%
! CAATTTTACCAACCCAAATT+GGG - chr1_2:37638662-37638681 Msa0069340:CDS 30.0%
!! ACATTGTTTTTCAACCTCTA+AGG - chr1_2:37638689-37638708 Msa0069340:CDS 30.0%
AACACTTTATTATGCCTTAG+AGG + chr1_2:37638706-37638725 Msa0069330:three_prime_UTR 30.0%
! TTTTCACCAAATAGATGTGT+CGG - chr1_2:37638724-37638743 Msa0069340:CDS 30.0%
! ATTTTTCCGACACATCTATT+TGG + chr1_2:37638733-37638752 Msa0069330:three_prime_UTR 30.0%
AAAGGGTTATAAGAATGTTG+AGG - chr1_2:37638774-37638793 Msa0069340:CDS 30.0%
TTGTTAAGATTACACACCTT+AGG + chr1_2:37638921-37638940 Msa0069330:three_prime_UTR 30.0%
! AAACCATAGATTGGTCAAAA+TGG - chr1_2:37639175-37639194 Msa0069340:intron 30.0%
CGACAATAAGATTTATTGAC+GGG + chr1_2:37639567-37639586 Msa0069330:intron 30.0%
ATTTCCATTATTCCTTTGTC+AGG - chr1_2:37639628-37639647 Msa0069340:intron 30.0%
TTGAAATGGTACTTAGACAA+AGG - chr1_2:37640073-37640092 Msa0069340:CDS 30.0%
ATTAGTCAAAGTTGTTGAGA+AGG + chr1_2:37638578-37638597 Msa0069330:three_prime_UTR 30.0%
! ACAAAATAAGCTACTTTGGA+GGG + chr1_2:37638608-37638627 Msa0069330:three_prime_UTR 30.0%
! AACAAAATAAGCTACTTTGG+AGG + chr1_2:37638609-37638628 Msa0069330:three_prime_UTR 30.0%
GTGAACAAAATAAGCTACTT+TGG + chr1_2:37638612-37638631 Msa0069330:three_prime_UTR 30.0%
! TCAATTTTACCAACCCAAAT+TGG - chr1_2:37638661-37638680 Msa0069340:CDS 30.0%
! CAATTTTACCAACCCAAATT+GGG - chr1_2:37638662-37638681 Msa0069340:CDS 30.0%
!! ACATTGTTTTTCAACCTCTA+AGG - chr1_2:37638689-37638708 Msa0069340:CDS 30.0%
AACACTTTATTATGCCTTAG+AGG + chr1_2:37638706-37638725 Msa0069330:three_prime_UTR 30.0%
! TTTTCACCAAATAGATGTGT+CGG - chr1_2:37638724-37638743 Msa0069340:CDS 30.0%
! ATTTTTCCGACACATCTATT+TGG + chr1_2:37638733-37638752 Msa0069330:three_prime_UTR 30.0%
AAAGGGTTATAAGAATGTTG+AGG - chr1_2:37638774-37638793 Msa0069340:CDS 30.0%
TTGTTAAGATTACACACCTT+AGG + chr1_2:37638921-37638940 Msa0069330:three_prime_UTR 30.0%
! AAACCATAGATTGGTCAAAA+TGG - chr1_2:37639175-37639194 Msa0069340:intron 30.0%
CGACAATAAGATTTATTGAC+GGG + chr1_2:37639567-37639586 Msa0069330:intron 30.0%
ATTTCCATTATTCCTTTGTC+AGG - chr1_2:37639628-37639647 Msa0069340:intron 30.0%
TTGAAATGGTACTTAGACAA+AGG - chr1_2:37640073-37640092 Msa0069340:CDS 30.0%
! ATGTTTTTCCCAATTTGGGT+TGG + chr1_2:37638673-37638692 Msa0069330:three_prime_UTR 35.0%
TAGATGTGTCGGAAAAATAG+AGG - chr1_2:37638735-37638754 Msa0069340:CDS 35.0%
!! AAATAGAGGCATCACTTAAG+TGG - chr1_2:37638749-37638768 Msa0069340:CDS 35.0%
!! AATAGAGGCATCACTTAAGT+GGG - chr1_2:37638750-37638769 Msa0069340:CDS 35.0%
! GAATGTTGAGGTTTTCAGTA+AGG - chr1_2:37638786-37638805 Msa0069340:CDS 35.0%
!! TATTTCACTGTTGCTTTGTC+TGG - chr1_2:37638877-37638896 Msa0069340:CDS 35.0%
GATCATTGATCAAGTACCTA+AGG - chr1_2:37638902-37638921 Msa0069340:CDS 35.0%
ACCTAATTTAAATAGGCTGG+TGG - chr1_2:37638981-37639000 Msa0069340:CDS 35.0%
TCCACCAGCCTATTTAAATT+AGG + chr1_2:37638985-37639004 Msa0069330:three_prime_UTR 35.0%
CAATCTATGGTTTCAGCATA+TGG + chr1_2:37639168-37639187 Msa0069330:intron 35.0%
CATATGCTGAAACCATAGAT+TGG - chr1_2:37639166-37639185 Msa0069340:intron 35.0%
!!! ATGCCATTTTGACCAATCTA+TGG + chr1_2:37639181-37639200 Msa0069330:intron 35.0%
! GTCAAAATGGCATGTTTTCT+GGG - chr1_2:37639188-37639207 Msa0069340:intron 35.0%
AATTATAAGCTTGCCAGTGA+TGG - chr1_2:37639249-37639268 Msa0069340:intron 35.0%
TTCAAGCAAAACAATCAACC+TGG + chr1_2:37639304-37639323 Msa0069330:intron 35.0%
TGCTTGAAGAAGAATGATGA+TGG - chr1_2:37639316-37639335 Msa0069340:intron 35.0%
CTTATTGTCGATCTTTAGTG+AGG - chr1_2:37639577-37639596 Msa0069340:intron 35.0%
! GCTTTTAACCAAACGTCTTA+GGG + chr1_2:37639732-37639751 Msa0069330:intron 35.0%
! TGCTTTTAACCAAACGTCTT+AGG + chr1_2:37639733-37639752 Msa0069330:intron 35.0%
!! AGGAGCATGAGATCAAATTT+TGG + chr1_2:37639785-37639804 Msa0069330:three_prime_UTR 35.0%
AAATTTGATCTCATGCTCCT+AGG - chr1_2:37639785-37639804 Msa0069340:CDS 35.0%
AAAGTAATTCTCTCTGATGG+TGG + chr1_2:37639908-37639927 Msa0069330:three_prime_UTR 35.0%
GTGAAAGTAATTCTCTCTGA+TGG + chr1_2:37639911-37639930 Msa0069330:three_prime_UTR 35.0%
AAAAGGTTTCACCTCTTGAA+GGG - chr1_2:37640047-37640066 Msa0069340:CDS 35.0%
! TGCTGCTTCAAAACTGTTTA+AGG - chr1_2:37640096-37640115 Msa0069340:CDS 35.0%
! ATGTTTTTCCCAATTTGGGT+TGG + chr1_2:37638673-37638692 Msa0069330:three_prime_UTR 35.0%
TAGATGTGTCGGAAAAATAG+AGG - chr1_2:37638735-37638754 Msa0069340:CDS 35.0%
!! AAATAGAGGCATCACTTAAG+TGG - chr1_2:37638749-37638768 Msa0069340:CDS 35.0%
!! AATAGAGGCATCACTTAAGT+GGG - chr1_2:37638750-37638769 Msa0069340:CDS 35.0%
! GAATGTTGAGGTTTTCAGTA+AGG - chr1_2:37638786-37638805 Msa0069340:CDS 35.0%
!! TATTTCACTGTTGCTTTGTC+TGG - chr1_2:37638877-37638896 Msa0069340:CDS 35.0%
GATCATTGATCAAGTACCTA+AGG - chr1_2:37638902-37638921 Msa0069340:CDS 35.0%
ACCTAATTTAAATAGGCTGG+TGG - chr1_2:37638981-37639000 Msa0069340:CDS 35.0%
TCCACCAGCCTATTTAAATT+AGG + chr1_2:37638985-37639004 Msa0069330:three_prime_UTR 35.0%
CAATCTATGGTTTCAGCATA+TGG + chr1_2:37639168-37639187 Msa0069330:intron 35.0%
CATATGCTGAAACCATAGAT+TGG - chr1_2:37639166-37639185 Msa0069340:intron 35.0%
!!! ATGCCATTTTGACCAATCTA+TGG + chr1_2:37639181-37639200 Msa0069330:intron 35.0%
! GTCAAAATGGCATGTTTTCT+GGG - chr1_2:37639188-37639207 Msa0069340:intron 35.0%
AATTATAAGCTTGCCAGTGA+TGG - chr1_2:37639249-37639268 Msa0069340:intron 35.0%
TTCAAGCAAAACAATCAACC+TGG + chr1_2:37639304-37639323 Msa0069330:intron 35.0%
TGCTTGAAGAAGAATGATGA+TGG - chr1_2:37639316-37639335 Msa0069340:intron 35.0%
CTTATTGTCGATCTTTAGTG+AGG - chr1_2:37639577-37639596 Msa0069340:intron 35.0%
! GCTTTTAACCAAACGTCTTA+GGG + chr1_2:37639732-37639751 Msa0069330:intron 35.0%
! TGCTTTTAACCAAACGTCTT+AGG + chr1_2:37639733-37639752 Msa0069330:intron 35.0%
!! AGGAGCATGAGATCAAATTT+TGG + chr1_2:37639785-37639804 Msa0069330:three_prime_UTR 35.0%
AAATTTGATCTCATGCTCCT+AGG - chr1_2:37639785-37639804 Msa0069340:CDS 35.0%
AAAGTAATTCTCTCTGATGG+TGG + chr1_2:37639908-37639927 Msa0069330:three_prime_UTR 35.0%
GTGAAAGTAATTCTCTCTGA+TGG + chr1_2:37639911-37639930 Msa0069330:three_prime_UTR 35.0%
AAAAGGTTTCACCTCTTGAA+GGG - chr1_2:37640047-37640066 Msa0069340:CDS 35.0%
! TGCTGCTTCAAAACTGTTTA+AGG - chr1_2:37640096-37640115 Msa0069340:CDS 35.0%
GCATCACTTAAGTGGGAAAA+GGG - chr1_2:37638757-37638776 Msa0069340:CDS 40.0%
! GGTCAAAATGGCATGTTTTC+TGG - chr1_2:37639187-37639206 Msa0069340:intron 40.0%
GGCACATGAGTTAATTACCT+TGG + chr1_2:37639283-37639302 Msa0069330:intron 40.0%
AGGTAATTAACTCATGTGCC+AGG - chr1_2:37639283-37639302 Msa0069340:intron 40.0%
TACTAGCATATCGTGACATG+TGG - chr1_2:37639418-37639437 Msa0069340:CDS 40.0%
ATTACGATATCATAGGTGCG+AGG - chr1_2:37639513-37639532 Msa0069340:CDS 40.0%
TAAACGTTGAGAGGAGGAAT+TGG + chr1_2:37639656-37639675 Msa0069330:intron 40.0%
TCAATGGCATAAACGTTGAG+AGG + chr1_2:37639665-37639684 Msa0069330:intron 40.0%
TGAAACAACCCTAAGACGTT+TGG - chr1_2:37639721-37639740 Msa0069340:intron 40.0%
AAAGATGCAACATGTCCATC+TGG + chr1_2:37639818-37639837 Msa0069330:three_prime_UTR 40.0%
CATGTTGCATCTTTATTCCC+AGG - chr1_2:37639824-37639843 Msa0069340:CDS 40.0%
TTCTCCAAGAGGATCAGAAA+TGG - chr1_2:37639855-37639874 Msa0069340:CDS 40.0%
TCTCCAAGAGGATCAGAAAT+GGG - chr1_2:37639856-37639875 Msa0069340:CDS 40.0%
TAACCCATTTCTGATCCTCT+TGG + chr1_2:37639862-37639881 Msa0069330:three_prime_UTR 40.0%
!!! ATTCTCTCTGATGGTGGTTT+TGG + chr1_2:37639902-37639921 Msa0069330:three_prime_UTR 40.0%
!! TTGGAAGAAGCATTGATCAC+TGG + chr1_2:37639938-37639957 Msa0069330:three_prime_UTR 40.0%
TGCTTCTTCCAATGTTGCAA+TGG - chr1_2:37639946-37639965 Msa0069340:CDS 40.0%
TTCTTCCAATGTTGCAATGG+TGG - chr1_2:37639949-37639968 Msa0069340:CDS 40.0%
TGTCATGTTGCCTGTTGAAA+AGG - chr1_2:37640030-37640049 Msa0069340:CDS 40.0%
! AGAGGTGAAACCTTTTCAAC+AGG + chr1_2:37640043-37640062 Msa0069330:three_prime_UTR 40.0%
GAAAAGGTTTCACCTCTTGA+AGG - chr1_2:37640046-37640065 Msa0069340:CDS 40.0%
AAAGGTTTCACCTCTTGAAG+GGG - chr1_2:37640048-37640067 Msa0069340:CDS 40.0%
CATTTCAATTCCCCTTCAAG+AGG + chr1_2:37640061-37640080 Msa0069330:three_prime_UTR 40.0%
CTCTTGAAGGGGAATTGAAA+TGG - chr1_2:37640059-37640078 Msa0069340:CDS 40.0%
GCATCACTTAAGTGGGAAAA+GGG - chr1_2:37638757-37638776 Msa0069340:CDS 40.0%
! GGTCAAAATGGCATGTTTTC+TGG - chr1_2:37639187-37639206 Msa0069340:intron 40.0%
GGCACATGAGTTAATTACCT+TGG + chr1_2:37639283-37639302 Msa0069330:intron 40.0%
AGGTAATTAACTCATGTGCC+AGG - chr1_2:37639283-37639302 Msa0069340:intron 40.0%
TACTAGCATATCGTGACATG+TGG - chr1_2:37639418-37639437 Msa0069340:CDS 40.0%
ATTACGATATCATAGGTGCG+AGG - chr1_2:37639513-37639532 Msa0069340:CDS 40.0%
TAAACGTTGAGAGGAGGAAT+TGG + chr1_2:37639656-37639675 Msa0069330:intron 40.0%
TCAATGGCATAAACGTTGAG+AGG + chr1_2:37639665-37639684 Msa0069330:intron 40.0%
TGAAACAACCCTAAGACGTT+TGG - chr1_2:37639721-37639740 Msa0069340:intron 40.0%
AAAGATGCAACATGTCCATC+TGG + chr1_2:37639818-37639837 Msa0069330:three_prime_UTR 40.0%
CATGTTGCATCTTTATTCCC+AGG - chr1_2:37639824-37639843 Msa0069340:CDS 40.0%
TTCTCCAAGAGGATCAGAAA+TGG - chr1_2:37639855-37639874 Msa0069340:CDS 40.0%
TCTCCAAGAGGATCAGAAAT+GGG - chr1_2:37639856-37639875 Msa0069340:CDS 40.0%
TAACCCATTTCTGATCCTCT+TGG + chr1_2:37639862-37639881 Msa0069330:three_prime_UTR 40.0%
!!! ATTCTCTCTGATGGTGGTTT+TGG + chr1_2:37639902-37639921 Msa0069330:three_prime_UTR 40.0%
!! TTGGAAGAAGCATTGATCAC+TGG + chr1_2:37639938-37639957 Msa0069330:three_prime_UTR 40.0%
TGCTTCTTCCAATGTTGCAA+TGG - chr1_2:37639946-37639965 Msa0069340:CDS 40.0%
TTCTTCCAATGTTGCAATGG+TGG - chr1_2:37639949-37639968 Msa0069340:CDS 40.0%
TGTCATGTTGCCTGTTGAAA+AGG - chr1_2:37640030-37640049 Msa0069340:CDS 40.0%
! AGAGGTGAAACCTTTTCAAC+AGG + chr1_2:37640043-37640062 Msa0069330:three_prime_UTR 40.0%
GAAAAGGTTTCACCTCTTGA+AGG - chr1_2:37640046-37640065 Msa0069340:CDS 40.0%
AAAGGTTTCACCTCTTGAAG+GGG - chr1_2:37640048-37640067 Msa0069340:CDS 40.0%
CATTTCAATTCCCCTTCAAG+AGG + chr1_2:37640061-37640080 Msa0069330:three_prime_UTR 40.0%
CTCTTGAAGGGGAATTGAAA+TGG - chr1_2:37640059-37640078 Msa0069340:CDS 40.0%
GGCATCACTTAAGTGGGAAA+AGG - chr1_2:37638756-37638775 Msa0069340:CDS 45.0%
! GGAACATCTTGCTGTGTCAT+TGG - chr1_2:37638807-37638826 Msa0069340:CDS 45.0%
!! TTCACTGTTGCTTTGTCTGG+TGG - chr1_2:37638880-37638899 Msa0069340:CDS 45.0%
CTTGGAGAGAAATCCATCAC+TGG + chr1_2:37639265-37639284 Msa0069330:intron 45.0%
!! CAGTGATGGATTTCTCTCCA+AGG - chr1_2:37639263-37639282 Msa0069340:intron 45.0%
GGCACCTGACAAAGGAATAA+TGG + chr1_2:37639635-37639654 Msa0069330:intron 45.0%
ATGGCATAAACGTTGAGAGG+AGG + chr1_2:37639662-37639681 Msa0069330:intron 45.0%
AGGTGGTAGGGAATCATCAA+TGG + chr1_2:37639681-37639700 Msa0069330:intron 45.0%
TAAACATCAGCTGCTCCATC+AGG + chr1_2:37639701-37639720 Msa0069330:intron 45.0%
TGATCTCATGCTCCTAGGTA+TGG - chr1_2:37639790-37639809 Msa0069340:CDS 45.0%
GATCTCATGCTCCTAGGTAT+GGG - chr1_2:37639791-37639810 Msa0069340:CDS 45.0%
AGTCACCACCATTGCAACAT+TGG + chr1_2:37639957-37639976 Msa0069330:three_prime_UTR 45.0%
! AATGTTGCAATGGTGGTGAC+TGG - chr1_2:37639956-37639975 Msa0069340:CDS 45.0%
CTCAAGTGCAGTGTAAACTG+CGG + chr1_2:37639996-37640015 Msa0069330:three_prime_UTR 45.0%
GGCATCACTTAAGTGGGAAA+AGG - chr1_2:37638756-37638775 Msa0069340:CDS 45.0%
! GGAACATCTTGCTGTGTCAT+TGG - chr1_2:37638807-37638826 Msa0069340:CDS 45.0%
!! TTCACTGTTGCTTTGTCTGG+TGG - chr1_2:37638880-37638899 Msa0069340:CDS 45.0%
CTTGGAGAGAAATCCATCAC+TGG + chr1_2:37639265-37639284 Msa0069330:intron 45.0%
!! CAGTGATGGATTTCTCTCCA+AGG - chr1_2:37639263-37639282 Msa0069340:intron 45.0%
GGCACCTGACAAAGGAATAA+TGG + chr1_2:37639635-37639654 Msa0069330:intron 45.0%
ATGGCATAAACGTTGAGAGG+AGG + chr1_2:37639662-37639681 Msa0069330:intron 45.0%
AGGTGGTAGGGAATCATCAA+TGG + chr1_2:37639681-37639700 Msa0069330:intron 45.0%
TAAACATCAGCTGCTCCATC+AGG + chr1_2:37639701-37639720 Msa0069330:intron 45.0%
TGATCTCATGCTCCTAGGTA+TGG - chr1_2:37639790-37639809 Msa0069340:CDS 45.0%
GATCTCATGCTCCTAGGTAT+GGG - chr1_2:37639791-37639810 Msa0069340:CDS 45.0%
AGTCACCACCATTGCAACAT+TGG + chr1_2:37639957-37639976 Msa0069330:three_prime_UTR 45.0%
! AATGTTGCAATGGTGGTGAC+TGG - chr1_2:37639956-37639975 Msa0069340:CDS 45.0%
CTCAAGTGCAGTGTAAACTG+CGG + chr1_2:37639996-37640015 Msa0069330:three_prime_UTR 45.0%
GAGGAATTGGCACCTGACAA+AGG + chr1_2:37639643-37639662 Msa0069330:intron 50.0%
GATGATTCCCTACCACCTGA+TGG - chr1_2:37639683-37639702 Msa0069340:intron 50.0%
! CTTGGAGAAGAGCATGACCT+GGG + chr1_2:37639844-37639863 Msa0069330:three_prime_UTR 50.0%
! TCTTGGAGAAGAGCATGACC+TGG + chr1_2:37639845-37639864 Msa0069330:three_prime_UTR 50.0%
AGGTCATGCTCTTCTCCAAG+AGG - chr1_2:37639844-37639863 Msa0069340:CDS 50.0%
!!! GTGGTTTTGGTGAGTCAGTG+AGG + chr1_2:37639889-37639908 Msa0069330:three_prime_UTR 50.0%
CGCAGTTTACACTGCACTTG+AGG - chr1_2:37639994-37640013 Msa0069340:CDS 50.0%
GAGGAATTGGCACCTGACAA+AGG + chr1_2:37639643-37639662 Msa0069330:intron 50.0%
GATGATTCCCTACCACCTGA+TGG - chr1_2:37639683-37639702 Msa0069340:intron 50.0%
! CTTGGAGAAGAGCATGACCT+GGG + chr1_2:37639844-37639863 Msa0069330:three_prime_UTR 50.0%
! TCTTGGAGAAGAGCATGACC+TGG + chr1_2:37639845-37639864 Msa0069330:three_prime_UTR 50.0%
AGGTCATGCTCTTCTCCAAG+AGG - chr1_2:37639844-37639863 Msa0069340:CDS 50.0%
!!! GTGGTTTTGGTGAGTCAGTG+AGG + chr1_2:37639889-37639908 Msa0069330:three_prime_UTR 50.0%
CGCAGTTTACACTGCACTTG+AGG - chr1_2:37639994-37640013 Msa0069340:CDS 50.0%
AGCTGCTCCATCAGGTGGTA+GGG + chr1_2:37639693-37639712 Msa0069330:intron 55.0%
ACATCAGCTGCTCCATCAGG+TGG + chr1_2:37639698-37639717 Msa0069330:intron 55.0%
CTCCTAGGTATGGGTCCAGA+TGG - chr1_2:37639800-37639819 Msa0069340:CDS 55.0%
GTCCATCTGGACCCATACCT+AGG + chr1_2:37639805-37639824 Msa0069330:three_prime_UTR 55.0%
AGCTGCTCCATCAGGTGGTA+GGG + chr1_2:37639693-37639712 Msa0069330:intron 55.0%
ACATCAGCTGCTCCATCAGG+TGG + chr1_2:37639698-37639717 Msa0069330:intron 55.0%
CTCCTAGGTATGGGTCCAGA+TGG - chr1_2:37639800-37639819 Msa0069340:CDS 55.0%
GTCCATCTGGACCCATACCT+AGG + chr1_2:37639805-37639824 Msa0069330:three_prime_UTR 55.0%
CAGCTGCTCCATCAGGTGGT+AGG + chr1_2:37639694-37639713 Msa0069330:intron 60.0%
! TGCAATGGTGGTGACTGGCG+CGG - chr1_2:37639961-37639980 Msa0069340:CDS 60.0%
CAGCTGCTCCATCAGGTGGT+AGG + chr1_2:37639694-37639713 Msa0069330:intron 60.0%
! TGCAATGGTGGTGACTGGCG+CGG - chr1_2:37639961-37639980 Msa0069340:CDS 60.0%
! GCAATGGTGGTGACTGGCGC+GGG - chr1_2:37639962-37639981 Msa0069340:CDS 65.0%
! GCAATGGTGGTGACTGGCGC+GGG - chr1_2:37639962-37639981 Msa0069340:CDS 65.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 37638571 37640123 37638571 ID=Msa0069340;Name=Msa0069340
chr1_2 mRNA 37638571 37640123 37638571 ID=Msa0069340-mRNA-1;Parent=Msa0069340;Name=Msa0069340-mRNA-1;_AED=0.31;_eAED=0.31;_QI=0|0|0|1|1|1|3|0|320
chr1_2 exon 37639771 37640123 37639771 ID=Msa0069340-mRNA-1:exon:11909;Parent=Msa0069340-mRNA-1
chr1_2 exon 37639410 37639545 37639410 ID=Msa0069340-mRNA-1:exon:11908;Parent=Msa0069340-mRNA-1
chr1_2 exon 37638571 37639044 37638571 ID=Msa0069340-mRNA-1:exon:11907;Parent=Msa0069340-mRNA-1
chr1_2 CDS 37639771 37640123 37639771 ID=Msa0069340-mRNA-1:cds;Parent=Msa0069340-mRNA-1
chr1_2 CDS 37639410 37639545 37639410 ID=Msa0069340-mRNA-1:cds;Parent=Msa0069340-mRNA-1
chr1_2 CDS 37638571 37639044 37638571 ID=Msa0069340-mRNA-1:cds;Parent=Msa0069340-mRNA-1
Gene Sequence

>Msa0069340

ATGGCCTTCTCAACAACTTTGACTAATTCATGCACCCTCCAAAGTAGCTTATTTTGTTCACAAAAATCAAATCCACTATTACTAAAGAAATCAATTTTACCAACCCAAATTGGGAAAAACATTGTTTTTCAACCTCTAAGGCATAATAAAGTGTTTTCACCAAATAGATGTGTCGGAAAAATAGAGGCATCACTTAAGTGGGAAAAGGGTTATAAGAATGTTGAGGTTTTCAGTAAGGAACATCTTGCTGTGTCATTGGCTTATGATGTTGCTCAACTTTCTAACAAATTCATCAAAGAAAGAGGTTATTTCACTGTTGCTTTGTCTGGTGGATCATTGATCAAGTACCTAAGGAAATTGGTTGAGTCTCCATATGCTGAAACCATAGATTGGTCAAAATGGCATGTTTTCTGGGTTGATGAGAGAGTTGTCCCAAAAGATAACTTAGAAAGTAATTATAAGCTTGCCAGTGATGGATTTCTCTCCAAGGTGCCAATTCCTCCTCTCAACGTTTATGCCATTGATGATTCCCTACCACCTGATGGAGCAGCTGATGTTTATGAAACAACCCTAAGACGTTTGGTTAAAAGCAATGTGATATCTACATCAACCAATGAATTACCAAAATTTGATCTCATGCTCCTAGGTATGGGTCCAGATGGACATGTTGCATCTTTATTCCCAGGTCATGCTCTTCTCCAAGAGGATCAGAAATGGGTTACTTTCCTCACTGACTCACCAAAACCACCATCAGAGAGAATTACTTTCACTTTTCCAGTGATCAATGCTTCTTCCAATGTTGCAATGGTGGTGACTGGCGCGGGTAAATCGACCGCAGTTTACACTGCACTTGAGGATGAGAAGAAAACTGTCATGTTGCCTGTTGAAAAGGTTTCACCTCTTGAAGGGGAATTGAAATGGTACTTAGACAAAGGTGCTGCTTCAAAACTGTTTAAGGAGTAA
Protein sequence

>Msa0069340

MAFSTTLTNSCTLQSSLFCSQKSNPLLLKKSILPTQIGKNIVFQPLRHNKVFSPNRCVGKIEASLKWEKGYKNVEVFSKEHLAVSLAYDVAQLSNKFIKERGYFTVALSGGSLIKYLRKLVESPYAETIDWSKWHVFWVDERVVPKDNLESNYKLASDGFLSKVPIPPLNVYAIDDSLPPDGAADVYETTLRRLVKSNVISTSTNELPKFDLMLLGMGPDGHVASLFPGHALLQEDQKWVTFLTDSPKPPSERITFTFPVINASSNVAMVVTGAGKSTAVYTALEDEKKTVMLPVEKVSPLEGELKWYLDKGAASKLFKE*