AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0104560

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0104560 MtrunA17_Chr1g0158811 97.188 320 9 0 1 320 1 320 0.0 639
Msa0104560 MtrunA17_Chr1g0158821 61.920 323 114 6 1 319 1 318 8.07e-133 380
Msa0104560 MtrunA17_Chr3g0127471 65.000 280 87 5 43 317 36 309 1.67e-120 348
Msa0104560 MtrunA17_Chr5g0419391 47.036 253 131 3 68 317 7 259 3.09e-77 237
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0104560 AT5G24400.1 62.903 248 87 3 72 317 81 325 5.60e-105 310
Msa0104560 AT3G49360.1 54.286 245 103 4 78 317 11 251 1.28e-85 258
Msa0104560 AT1G13700.2 50.000 242 117 2 80 317 20 261 1.16e-81 248
Msa0104560 AT1G13700.3 50.000 242 117 2 80 317 20 261 1.16e-81 248
Msa0104560 AT1G13700.1 50.000 242 117 2 80 317 20 261 1.16e-81 248
Msa0104560 AT5G24410.1 52.653 245 107 4 78 317 17 257 6.53e-80 244
Msa0104560 AT5G24420.1 51.837 245 110 4 78 317 11 252 7.23e-79 241

Find 67 sgRNAs with CRISPR-Local

Find 236 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTGCTTCAAAACTGTTTA+AGG 0.136078 1_3:-12504838 Msa0104560:CDS
ATTCTCTCTGATGGTGGTTT+TGG 0.280005 1_3:+12505035 Msa0104550:three_prime_UTR
TTACTTTCTAAGTTATCTTT+TGG 0.280021 1_3:+12505706 Msa0104550:three_prime_UTR
AACACTTTATTATGCCTTAG+AGG 0.288583 1_3:+12506231 Msa0104550:three_prime_UTR
AAACCATAGATTGGTCAAAA+TGG 0.294312 1_3:-12505759 Msa0104560:CDS
AGGAGCATGAGATCAAATTT+TGG 0.311450 1_3:+12505152 Msa0104550:three_prime_UTR
ATTGATTTCTTTAGTAATAG+TGG 0.312473 1_3:+12506291 Msa0104550:three_prime_UTR
TATTTCACTGTTGCTTTGTC+TGG 0.315803 1_3:-12506057 Msa0104560:CDS
CAATCTATGGTTTCAGCATA+TGG 0.367849 1_3:+12505769 Msa0104550:three_prime_UTR
GTGAACAAAATAAGCTACTT+TGG 0.384187 1_3:+12506325 Msa0104550:three_prime_UTR
TGATCTCATGCTCCTAGGTA+TGG 0.406465 1_3:-12505144 Msa0104560:CDS
TGAAACAACCCTAAGACGTT+TGG 0.412525 1_3:-12505213 Msa0104560:CDS
GGAACATCTTGCTGTGTCAT+TGG 0.418210 1_3:-12506127 Msa0104560:CDS
TTCTCCAAGAGGATCAGAAA+TGG 0.419423 1_3:-12505079 Msa0104560:CDS
AACAAATTCATCAAAGAAAG+AGG 0.421608 1_3:-12506081 Msa0104560:CDS
TTCACTGTTGCTTTGTCTGG+TGG 0.432382 1_3:-12506054 Msa0104560:CDS
GGCATCACTTAAGTGGGAAA+AGG 0.443000 1_3:-12506178 Msa0104560:CDS
TAAACGTTGAGAGGAGGAAT+TGG 0.449024 1_3:+12505281 Msa0104550:three_prime_UTR
TAACCCATTTCTGATCCTCT+TGG 0.454962 1_3:+12505075 Msa0104550:three_prime_UTR
GAAAAGGTTTCACCTCTTGA+AGG 0.456560 1_3:-12504888 Msa0104560:CDS
CATGTTGCATCTTTATTCCC+AGG 0.457125 1_3:-12505110 Msa0104560:CDS
CTCTTGAAGGGGAATTGAAA+TGG 0.460789 1_3:-12504875 Msa0104560:CDS
AAAGATGCAACATGTCCATC+TGG 0.461834 1_3:+12505119 Msa0104550:three_prime_UTR
TAAACATCAGCTGCTCCATC+AGG 0.473530 1_3:+12505236 Msa0104550:three_prime_UTR
AATTATAAGCTTGCCAGTGA+TGG 0.484242 1_3:-12505685 Msa0104560:CDS
GATCTCATGCTCCTAGGTAT+GGG 0.491824 1_3:-12505143 Msa0104560:CDS
TGTCATGTTGCCTGTTGAAA+AGG 0.494449 1_3:-12504904 Msa0104560:CDS
AAATAGAGGCATCACTTAAG+TGG 0.496228 1_3:-12506185 Msa0104560:CDS
CGCAGTTTACACTGCACTTG+AGG 0.500062 1_3:-12504940 Msa0104560:CDS
GCATCACTTAAGTGGGAAAA+GGG 0.504570 1_3:-12506177 Msa0104560:CDS
TCTCCAAGAGGATCAGAAAT+GGG 0.506655 1_3:-12505078 Msa0104560:CDS
CAGCTGCTCCATCAGGTGGT+AGG 0.508616 1_3:+12505243 Msa0104550:three_prime_UTR
AAATTTGATCTCATGCTCCT+AGG 0.509138 1_3:-12505149 Msa0104560:CDS
AAAAGGTTTCACCTCTTGAA+GGG 0.515655 1_3:-12504887 Msa0104560:CDS
AACAAAATAAGCTACTTTGG+AGG 0.518255 1_3:+12506328 Msa0104550:three_prime_UTR
AGTCACCACCATTGCAACAT+TGG 0.519798 1_3:+12504980 Msa0104550:three_prime_UTR
CATATGCTGAAACCATAGAT+TGG 0.520043 1_3:-12505768 Msa0104560:CDS
GATCATTGATCAAGTACCTA+AGG 0.528998 1_3:-12506032 Msa0104560:intron
AGCTGCTCCATCAGGTGGTA+GGG 0.529718 1_3:+12505244 Msa0104550:three_prime_UTR
GTGAAAGTAATTCTCTCTGA+TGG 0.540458 1_3:+12505026 Msa0104550:three_prime_UTR
TTGGAAGAAGCATTGATCAC+TGG 0.541052 1_3:+12504999 Msa0104550:three_prime_UTR
ATTAGTCAAAGTTGTTGAGA+AGG 0.549232 1_3:+12506359 Msa0104550:three_prime_UTR
GCAATGGTGGTGACTGGCGC+GGG 0.551673 1_3:-12504972 Msa0104560:CDS
CTCCTAGGTATGGGTCCAGA+TGG 0.553952 1_3:-12505134 Msa0104560:CDS
AATAGAGGCATCACTTAAGT+GGG 0.554935 1_3:-12506184 Msa0104560:CDS
TTGAAATGGTACTTAGACAA+AGG 0.555841 1_3:-12504861 Msa0104560:CDS
TGCTTCTTCCAATGTTGCAA+TGG 0.565044 1_3:-12504988 Msa0104560:CDS
CATTTCAATTCCCCTTCAAG+AGG 0.566687 1_3:+12504876 Msa0104550:three_prime_UTR
GATGATTCCCTACCACCTGA+TGG 0.566852 1_3:-12505251 Msa0104560:CDS
AATGTTGCAATGGTGGTGAC+TGG 0.569664 1_3:-12504978 Msa0104560:CDS
AGGTGGTAGGGAATCATCAA+TGG 0.569674 1_3:+12505256 Msa0104550:three_prime_UTR
TTCTTCCAATGTTGCAATGG+TGG 0.573190 1_3:-12504985 Msa0104560:CDS
AAAGGGTTATAAGAATGTTG+AGG 0.580239 1_3:-12506160 Msa0104560:CDS
TGCAATGGTGGTGACTGGCG+CGG 0.584998 1_3:-12504973 Msa0104560:CDS
ACAAAATAAGCTACTTTGGA+GGG 0.598728 1_3:+12506329 Msa0104550:three_prime_UTR
AAAGGTTTCACCTCTTGAAG+GGG 0.598919 1_3:-12504886 Msa0104560:CDS
CTTGGAGAGAAATCCATCAC+TGG 0.601315 1_3:+12505672 Msa0104550:three_prime_UTR
CTTGGAGAAGAGCATGACCT+GGG 0.611662 1_3:+12505093 Msa0104550:three_prime_UTR
AAAGTAATTCTCTCTGATGG+TGG 0.620404 1_3:+12505029 Msa0104550:three_prime_UTR
CAGTGATGGATTTCTCTCCA+AGG 0.622114 1_3:-12505671 Msa0104560:intron
TCTTGGAGAAGAGCATGACC+TGG 0.622800 1_3:+12505092 Msa0104550:three_prime_UTR
TCAATGGCATAAACGTTGAG+AGG 0.637656 1_3:+12505272 Msa0104550:three_prime_UTR
ATGGCATAAACGTTGAGAGG+AGG 0.640971 1_3:+12505275 Msa0104550:three_prime_UTR
ACATCAGCTGCTCCATCAGG+TGG 0.642046 1_3:+12505239 Msa0104550:three_prime_UTR
AGGTCATGCTCTTCTCCAAG+AGG 0.654284 1_3:-12505090 Msa0104560:CDS
GTCCATCTGGACCCATACCT+AGG 0.668407 1_3:+12505132 Msa0104550:three_prime_UTR
CTCAAGTGCAGTGTAAACTG+CGG 0.695035 1_3:+12504941 Msa0104550:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAGTAACATTTATATTAAA+AGG + chr1_3:12505632-12505651 Msa0104550:intron 10.0%
!! ATAGTAACATTTATATTAAA+AGG + chr1_3:12505632-12505651 Msa0104550:intron 10.0%
!! TTTGTTAACCTAATTTAAAT+AGG - chr1_3:12505236-12505255 Msa0104560:CDS 15.0%
!!! ATATGATTTTAATGTTACAT+AGG - chr1_3:12505269-12505288 Msa0104560:CDS 15.0%
!!! CAATTTTATGTTTTTATTTC+AGG - chr1_3:12505389-12505408 Msa0104560:intron 15.0%
!! TTTGTTAACCTAATTTAAAT+AGG - chr1_3:12505236-12505255 Msa0104560:CDS 15.0%
!!! ATATGATTTTAATGTTACAT+AGG - chr1_3:12505269-12505288 Msa0104560:CDS 15.0%
!!! CAATTTTATGTTTTTATTTC+AGG - chr1_3:12505389-12505408 Msa0104560:intron 15.0%
!!! ATTGATTTCTTTAGTAATAG+TGG + chr1_3:12504908-12504927 Msa0104550:three_prime_UTR 20.0%
!!! ATAGGTTGGTATAATTTAAA+TGG - chr1_3:12505287-12505306 Msa0104560:CDS 20.0%
!! ATTTAAATGGAATTGTGATT+AGG - chr1_3:12505300-12505319 Msa0104560:intron 20.0%
!!! CACCTTAATTATTTATTTCT+AGG - chr1_3:12505343-12505362 Msa0104560:intron 20.0%
!! AGCCTAGAAATAAATAATTA+AGG + chr1_3:12505348-12505367 Msa0104550:intron 20.0%
!!! ATGTTTTTATTTCAGGAAAT+TGG - chr1_3:12505396-12505415 Msa0104560:intron 20.0%
!!! TACTTTCTAAGTTATCTTTT+GGG + chr1_3:12505492-12505511 Msa0104550:intron 20.0%
!!! TTACTTTCTAAGTTATCTTT+TGG + chr1_3:12505493-12505512 Msa0104550:intron 20.0%
!!! GAAATTAATCATTTTGATGT+TGG - chr1_3:12505605-12505624 Msa0104560:intron 20.0%
!! ATGATATCGTAATTAGATAA+GGG + chr1_3:12505768-12505787 Msa0104550:three_prime_UTR 20.0%
!! TATGATATCGTAATTAGATA+AGG + chr1_3:12505769-12505788 Msa0104550:three_prime_UTR 20.0%
!! TTATCTAATTACGATATCAT+AGG - chr1_3:12505768-12505787 Msa0104560:CDS 20.0%
!!! ATCAAATTTTGGTAATTCAT+TGG + chr1_3:12506036-12506055 Msa0104550:three_prime_UTR 20.0%
!!! ATTGATTTCTTTAGTAATAG+TGG + chr1_3:12504908-12504927 Msa0104550:three_prime_UTR 20.0%
!!! ATAGGTTGGTATAATTTAAA+TGG - chr1_3:12505287-12505306 Msa0104560:CDS 20.0%
!! ATTTAAATGGAATTGTGATT+AGG - chr1_3:12505300-12505319 Msa0104560:intron 20.0%
!!! CACCTTAATTATTTATTTCT+AGG - chr1_3:12505343-12505362 Msa0104560:intron 20.0%
!! AGCCTAGAAATAAATAATTA+AGG + chr1_3:12505348-12505367 Msa0104550:intron 20.0%
!!! ATGTTTTTATTTCAGGAAAT+TGG - chr1_3:12505396-12505415 Msa0104560:intron 20.0%
!!! TACTTTCTAAGTTATCTTTT+GGG + chr1_3:12505492-12505511 Msa0104550:intron 20.0%
!!! TTACTTTCTAAGTTATCTTT+TGG + chr1_3:12505493-12505512 Msa0104550:intron 20.0%
!!! GAAATTAATCATTTTGATGT+TGG - chr1_3:12505605-12505624 Msa0104560:intron 20.0%
!! ATGATATCGTAATTAGATAA+GGG + chr1_3:12505768-12505787 Msa0104550:three_prime_UTR 20.0%
!! TATGATATCGTAATTAGATA+AGG + chr1_3:12505769-12505788 Msa0104550:three_prime_UTR 20.0%
!! TTATCTAATTACGATATCAT+AGG - chr1_3:12505768-12505787 Msa0104560:CDS 20.0%
!!! ATCAAATTTTGGTAATTCAT+TGG + chr1_3:12506036-12506055 Msa0104550:three_prime_UTR 20.0%
!! AACAATGTTTTTCCCAATTT+GGG + chr1_3:12504939-12504958 Msa0104550:three_prime_UTR 25.0%
!! AAACAATGTTTTTCCCAATT+TGG + chr1_3:12504940-12504959 Msa0104550:three_prime_UTR 25.0%
! AACAAATTCATCAAAGAAAG+AGG - chr1_3:12505115-12505134 Msa0104560:CDS 25.0%
! TTAACCTAATTTAAATAGGC+TGG - chr1_3:12505240-12505259 Msa0104560:CDS 25.0%
!!! GATTTTAATGTTACATAGGT+TGG - chr1_3:12505273-12505292 Msa0104560:CDS 25.0%
! AAATCGAACAACTAATCAAA+TGG + chr1_3:12505726-12505745 Msa0104550:three_prime_UTR 25.0%
! GAATTAACAATCATCAAACT+TGG + chr1_3:12505807-12505826 Msa0104550:intron 25.0%
! TCGACAATAAGATTTATTGA+CGG + chr1_3:12505830-12505849 Msa0104550:intron 25.0%
!! AACAATGTTTTTCCCAATTT+GGG + chr1_3:12504939-12504958 Msa0104550:three_prime_UTR 25.0%
!! AAACAATGTTTTTCCCAATT+TGG + chr1_3:12504940-12504959 Msa0104550:three_prime_UTR 25.0%
! AACAAATTCATCAAAGAAAG+AGG - chr1_3:12505115-12505134 Msa0104560:CDS 25.0%
! TTAACCTAATTTAAATAGGC+TGG - chr1_3:12505240-12505259 Msa0104560:CDS 25.0%
!!! GATTTTAATGTTACATAGGT+TGG - chr1_3:12505273-12505292 Msa0104560:CDS 25.0%
! AAATCGAACAACTAATCAAA+TGG + chr1_3:12505726-12505745 Msa0104550:three_prime_UTR 25.0%
! GAATTAACAATCATCAAACT+TGG + chr1_3:12505807-12505826 Msa0104550:intron 25.0%
! TCGACAATAAGATTTATTGA+CGG + chr1_3:12505830-12505849 Msa0104550:intron 25.0%
ATTAGTCAAAGTTGTTGAGA+AGG + chr1_3:12504840-12504859 Msa0104550:three_prime_UTR 30.0%
! ACAAAATAAGCTACTTTGGA+GGG + chr1_3:12504870-12504889 Msa0104550:three_prime_UTR 30.0%
! AACAAAATAAGCTACTTTGG+AGG + chr1_3:12504871-12504890 Msa0104550:three_prime_UTR 30.0%
GTGAACAAAATAAGCTACTT+TGG + chr1_3:12504874-12504893 Msa0104550:three_prime_UTR 30.0%
! TCAATTTTACCAACCCAAAT+TGG - chr1_3:12504923-12504942 Msa0104560:CDS 30.0%
! CAATTTTACCAACCCAAATT+GGG - chr1_3:12504924-12504943 Msa0104560:CDS 30.0%
!! ACATTGTTTTTCAACCTCTA+AGG - chr1_3:12504951-12504970 Msa0104560:CDS 30.0%
AACACTTTATTATGCCTTAG+AGG + chr1_3:12504968-12504987 Msa0104550:three_prime_UTR 30.0%
! TTTTCACCAAATAGATGTGT+CGG - chr1_3:12504986-12505005 Msa0104560:CDS 30.0%
! ATTTTTCCGACACATCTATT+TGG + chr1_3:12504995-12505014 Msa0104550:three_prime_UTR 30.0%
AAAGGGTTATAAGAATGTTG+AGG - chr1_3:12505036-12505055 Msa0104560:CDS 30.0%
TTGTTAAGATTACACACCTT+AGG + chr1_3:12505183-12505202 Msa0104550:three_prime_UTR 30.0%
! AAACCATAGATTGGTCAAAA+TGG - chr1_3:12505437-12505456 Msa0104560:intron 30.0%
CGACAATAAGATTTATTGAC+GGG + chr1_3:12505829-12505848 Msa0104550:intron 30.0%
ATTTCCATTATTCCTTTGTC+AGG - chr1_3:12505890-12505909 Msa0104560:intron 30.0%
TTGAAATGGTACTTAGACAA+AGG - chr1_3:12506335-12506354 Msa0104560:CDS 30.0%
ATTAGTCAAAGTTGTTGAGA+AGG + chr1_3:12504840-12504859 Msa0104550:three_prime_UTR 30.0%
! ACAAAATAAGCTACTTTGGA+GGG + chr1_3:12504870-12504889 Msa0104550:three_prime_UTR 30.0%
! AACAAAATAAGCTACTTTGG+AGG + chr1_3:12504871-12504890 Msa0104550:three_prime_UTR 30.0%
GTGAACAAAATAAGCTACTT+TGG + chr1_3:12504874-12504893 Msa0104550:three_prime_UTR 30.0%
! TCAATTTTACCAACCCAAAT+TGG - chr1_3:12504923-12504942 Msa0104560:CDS 30.0%
! CAATTTTACCAACCCAAATT+GGG - chr1_3:12504924-12504943 Msa0104560:CDS 30.0%
!! ACATTGTTTTTCAACCTCTA+AGG - chr1_3:12504951-12504970 Msa0104560:CDS 30.0%
AACACTTTATTATGCCTTAG+AGG + chr1_3:12504968-12504987 Msa0104550:three_prime_UTR 30.0%
! TTTTCACCAAATAGATGTGT+CGG - chr1_3:12504986-12505005 Msa0104560:CDS 30.0%
! ATTTTTCCGACACATCTATT+TGG + chr1_3:12504995-12505014 Msa0104550:three_prime_UTR 30.0%
AAAGGGTTATAAGAATGTTG+AGG - chr1_3:12505036-12505055 Msa0104560:CDS 30.0%
TTGTTAAGATTACACACCTT+AGG + chr1_3:12505183-12505202 Msa0104550:three_prime_UTR 30.0%
! AAACCATAGATTGGTCAAAA+TGG - chr1_3:12505437-12505456 Msa0104560:intron 30.0%
CGACAATAAGATTTATTGAC+GGG + chr1_3:12505829-12505848 Msa0104550:intron 30.0%
ATTTCCATTATTCCTTTGTC+AGG - chr1_3:12505890-12505909 Msa0104560:intron 30.0%
TTGAAATGGTACTTAGACAA+AGG - chr1_3:12506335-12506354 Msa0104560:CDS 30.0%
! ATGTTTTTCCCAATTTGGGT+TGG + chr1_3:12504935-12504954 Msa0104550:three_prime_UTR 35.0%
TAGATGTGTCGGAAAAATAG+AGG - chr1_3:12504997-12505016 Msa0104560:CDS 35.0%
!! AAATAGAGGCATCACTTAAG+TGG - chr1_3:12505011-12505030 Msa0104560:CDS 35.0%
!! AATAGAGGCATCACTTAAGT+GGG - chr1_3:12505012-12505031 Msa0104560:CDS 35.0%
! GAATGTTGAGGTTTTCAGTA+AGG - chr1_3:12505048-12505067 Msa0104560:CDS 35.0%
!! TATTTCACTGTTGCTTTGTC+TGG - chr1_3:12505139-12505158 Msa0104560:CDS 35.0%
GATCATTGATCAAGTACCTA+AGG - chr1_3:12505164-12505183 Msa0104560:CDS 35.0%
ACCTAATTTAAATAGGCTGG+TGG - chr1_3:12505243-12505262 Msa0104560:CDS 35.0%
TCCACCAGCCTATTTAAATT+AGG + chr1_3:12505247-12505266 Msa0104550:three_prime_UTR 35.0%
CAATCTATGGTTTCAGCATA+TGG + chr1_3:12505430-12505449 Msa0104550:intron 35.0%
CATATGCTGAAACCATAGAT+TGG - chr1_3:12505428-12505447 Msa0104560:intron 35.0%
!!! ATGCCATTTTGACCAATCTA+TGG + chr1_3:12505443-12505462 Msa0104550:intron 35.0%
! GTCAAAATGGCATGTTTTCT+GGG - chr1_3:12505450-12505469 Msa0104560:intron 35.0%
AATTATAAGCTTGCCAGTGA+TGG - chr1_3:12505511-12505530 Msa0104560:intron 35.0%
TTCAAGCAAAACAATCAACC+TGG + chr1_3:12505566-12505585 Msa0104550:intron 35.0%
TGCTTGAAGAAGAATGATGA+TGG - chr1_3:12505578-12505597 Msa0104560:intron 35.0%
CTTATTGTCGATCTTTAGTG+AGG - chr1_3:12505839-12505858 Msa0104560:intron 35.0%
! GCTTTTAACCAAACGTCTTA+GGG + chr1_3:12505994-12506013 Msa0104550:intron 35.0%
! TGCTTTTAACCAAACGTCTT+AGG + chr1_3:12505995-12506014 Msa0104550:intron 35.0%
!! AGGAGCATGAGATCAAATTT+TGG + chr1_3:12506047-12506066 Msa0104550:three_prime_UTR 35.0%
AAATTTGATCTCATGCTCCT+AGG - chr1_3:12506047-12506066 Msa0104560:CDS 35.0%
AAAGTAATTCTCTCTGATGG+TGG + chr1_3:12506170-12506189 Msa0104550:three_prime_UTR 35.0%
GTGAAAGTAATTCTCTCTGA+TGG + chr1_3:12506173-12506192 Msa0104550:three_prime_UTR 35.0%
AAAAGGTTTCACCTCTTGAA+GGG - chr1_3:12506309-12506328 Msa0104560:CDS 35.0%
! TGCTGCTTCAAAACTGTTTA+AGG - chr1_3:12506358-12506377 Msa0104560:CDS 35.0%
! ATGTTTTTCCCAATTTGGGT+TGG + chr1_3:12504935-12504954 Msa0104550:three_prime_UTR 35.0%
TAGATGTGTCGGAAAAATAG+AGG - chr1_3:12504997-12505016 Msa0104560:CDS 35.0%
!! AAATAGAGGCATCACTTAAG+TGG - chr1_3:12505011-12505030 Msa0104560:CDS 35.0%
!! AATAGAGGCATCACTTAAGT+GGG - chr1_3:12505012-12505031 Msa0104560:CDS 35.0%
! GAATGTTGAGGTTTTCAGTA+AGG - chr1_3:12505048-12505067 Msa0104560:CDS 35.0%
!! TATTTCACTGTTGCTTTGTC+TGG - chr1_3:12505139-12505158 Msa0104560:CDS 35.0%
GATCATTGATCAAGTACCTA+AGG - chr1_3:12505164-12505183 Msa0104560:CDS 35.0%
ACCTAATTTAAATAGGCTGG+TGG - chr1_3:12505243-12505262 Msa0104560:CDS 35.0%
TCCACCAGCCTATTTAAATT+AGG + chr1_3:12505247-12505266 Msa0104550:three_prime_UTR 35.0%
CAATCTATGGTTTCAGCATA+TGG + chr1_3:12505430-12505449 Msa0104550:intron 35.0%
CATATGCTGAAACCATAGAT+TGG - chr1_3:12505428-12505447 Msa0104560:intron 35.0%
!!! ATGCCATTTTGACCAATCTA+TGG + chr1_3:12505443-12505462 Msa0104550:intron 35.0%
! GTCAAAATGGCATGTTTTCT+GGG - chr1_3:12505450-12505469 Msa0104560:intron 35.0%
AATTATAAGCTTGCCAGTGA+TGG - chr1_3:12505511-12505530 Msa0104560:intron 35.0%
TTCAAGCAAAACAATCAACC+TGG + chr1_3:12505566-12505585 Msa0104550:intron 35.0%
TGCTTGAAGAAGAATGATGA+TGG - chr1_3:12505578-12505597 Msa0104560:intron 35.0%
CTTATTGTCGATCTTTAGTG+AGG - chr1_3:12505839-12505858 Msa0104560:intron 35.0%
! GCTTTTAACCAAACGTCTTA+GGG + chr1_3:12505994-12506013 Msa0104550:intron 35.0%
! TGCTTTTAACCAAACGTCTT+AGG + chr1_3:12505995-12506014 Msa0104550:intron 35.0%
!! AGGAGCATGAGATCAAATTT+TGG + chr1_3:12506047-12506066 Msa0104550:three_prime_UTR 35.0%
AAATTTGATCTCATGCTCCT+AGG - chr1_3:12506047-12506066 Msa0104560:CDS 35.0%
AAAGTAATTCTCTCTGATGG+TGG + chr1_3:12506170-12506189 Msa0104550:three_prime_UTR 35.0%
GTGAAAGTAATTCTCTCTGA+TGG + chr1_3:12506173-12506192 Msa0104550:three_prime_UTR 35.0%
AAAAGGTTTCACCTCTTGAA+GGG - chr1_3:12506309-12506328 Msa0104560:CDS 35.0%
! TGCTGCTTCAAAACTGTTTA+AGG - chr1_3:12506358-12506377 Msa0104560:CDS 35.0%
GCATCACTTAAGTGGGAAAA+GGG - chr1_3:12505019-12505038 Msa0104560:CDS 40.0%
! GGTCAAAATGGCATGTTTTC+TGG - chr1_3:12505449-12505468 Msa0104560:intron 40.0%
GGCACATGAGTTAATTACCT+TGG + chr1_3:12505545-12505564 Msa0104550:intron 40.0%
AGGTAATTAACTCATGTGCC+AGG - chr1_3:12505545-12505564 Msa0104560:intron 40.0%
TACTAGCATATCGTGACATG+TGG - chr1_3:12505680-12505699 Msa0104560:CDS 40.0%
ATTACGATATCATAGGTGCG+AGG - chr1_3:12505775-12505794 Msa0104560:CDS 40.0%
TAAACGTTGAGAGGAGGAAT+TGG + chr1_3:12505918-12505937 Msa0104550:intron 40.0%
TCAATGGCATAAACGTTGAG+AGG + chr1_3:12505927-12505946 Msa0104550:intron 40.0%
TGAAACAACCCTAAGACGTT+TGG - chr1_3:12505983-12506002 Msa0104560:intron 40.0%
AAAGATGCAACATGTCCATC+TGG + chr1_3:12506080-12506099 Msa0104550:three_prime_UTR 40.0%
CATGTTGCATCTTTATTCCC+AGG - chr1_3:12506086-12506105 Msa0104560:CDS 40.0%
TTCTCCAAGAGGATCAGAAA+TGG - chr1_3:12506117-12506136 Msa0104560:CDS 40.0%
TCTCCAAGAGGATCAGAAAT+GGG - chr1_3:12506118-12506137 Msa0104560:CDS 40.0%
TAACCCATTTCTGATCCTCT+TGG + chr1_3:12506124-12506143 Msa0104550:three_prime_UTR 40.0%
!!! ATTCTCTCTGATGGTGGTTT+TGG + chr1_3:12506164-12506183 Msa0104550:three_prime_UTR 40.0%
!! TTGGAAGAAGCATTGATCAC+TGG + chr1_3:12506200-12506219 Msa0104550:three_prime_UTR 40.0%
TGCTTCTTCCAATGTTGCAA+TGG - chr1_3:12506208-12506227 Msa0104560:CDS 40.0%
TTCTTCCAATGTTGCAATGG+TGG - chr1_3:12506211-12506230 Msa0104560:CDS 40.0%
TGTCATGTTGCCTGTTGAAA+AGG - chr1_3:12506292-12506311 Msa0104560:CDS 40.0%
! AGAGGTGAAACCTTTTCAAC+AGG + chr1_3:12506305-12506324 Msa0104550:three_prime_UTR 40.0%
GAAAAGGTTTCACCTCTTGA+AGG - chr1_3:12506308-12506327 Msa0104560:CDS 40.0%
AAAGGTTTCACCTCTTGAAG+GGG - chr1_3:12506310-12506329 Msa0104560:CDS 40.0%
CATTTCAATTCCCCTTCAAG+AGG + chr1_3:12506323-12506342 Msa0104550:three_prime_UTR 40.0%
CTCTTGAAGGGGAATTGAAA+TGG - chr1_3:12506321-12506340 Msa0104560:CDS 40.0%
GCATCACTTAAGTGGGAAAA+GGG - chr1_3:12505019-12505038 Msa0104560:CDS 40.0%
! GGTCAAAATGGCATGTTTTC+TGG - chr1_3:12505449-12505468 Msa0104560:intron 40.0%
GGCACATGAGTTAATTACCT+TGG + chr1_3:12505545-12505564 Msa0104550:intron 40.0%
AGGTAATTAACTCATGTGCC+AGG - chr1_3:12505545-12505564 Msa0104560:intron 40.0%
TACTAGCATATCGTGACATG+TGG - chr1_3:12505680-12505699 Msa0104560:CDS 40.0%
ATTACGATATCATAGGTGCG+AGG - chr1_3:12505775-12505794 Msa0104560:CDS 40.0%
TAAACGTTGAGAGGAGGAAT+TGG + chr1_3:12505918-12505937 Msa0104550:intron 40.0%
TCAATGGCATAAACGTTGAG+AGG + chr1_3:12505927-12505946 Msa0104550:intron 40.0%
TGAAACAACCCTAAGACGTT+TGG - chr1_3:12505983-12506002 Msa0104560:intron 40.0%
AAAGATGCAACATGTCCATC+TGG + chr1_3:12506080-12506099 Msa0104550:three_prime_UTR 40.0%
CATGTTGCATCTTTATTCCC+AGG - chr1_3:12506086-12506105 Msa0104560:CDS 40.0%
TTCTCCAAGAGGATCAGAAA+TGG - chr1_3:12506117-12506136 Msa0104560:CDS 40.0%
TCTCCAAGAGGATCAGAAAT+GGG - chr1_3:12506118-12506137 Msa0104560:CDS 40.0%
TAACCCATTTCTGATCCTCT+TGG + chr1_3:12506124-12506143 Msa0104550:three_prime_UTR 40.0%
!!! ATTCTCTCTGATGGTGGTTT+TGG + chr1_3:12506164-12506183 Msa0104550:three_prime_UTR 40.0%
!! TTGGAAGAAGCATTGATCAC+TGG + chr1_3:12506200-12506219 Msa0104550:three_prime_UTR 40.0%
TGCTTCTTCCAATGTTGCAA+TGG - chr1_3:12506208-12506227 Msa0104560:CDS 40.0%
TTCTTCCAATGTTGCAATGG+TGG - chr1_3:12506211-12506230 Msa0104560:CDS 40.0%
TGTCATGTTGCCTGTTGAAA+AGG - chr1_3:12506292-12506311 Msa0104560:CDS 40.0%
! AGAGGTGAAACCTTTTCAAC+AGG + chr1_3:12506305-12506324 Msa0104550:three_prime_UTR 40.0%
GAAAAGGTTTCACCTCTTGA+AGG - chr1_3:12506308-12506327 Msa0104560:CDS 40.0%
AAAGGTTTCACCTCTTGAAG+GGG - chr1_3:12506310-12506329 Msa0104560:CDS 40.0%
CATTTCAATTCCCCTTCAAG+AGG + chr1_3:12506323-12506342 Msa0104550:three_prime_UTR 40.0%
CTCTTGAAGGGGAATTGAAA+TGG - chr1_3:12506321-12506340 Msa0104560:CDS 40.0%
GGCATCACTTAAGTGGGAAA+AGG - chr1_3:12505018-12505037 Msa0104560:CDS 45.0%
! GGAACATCTTGCTGTGTCAT+TGG - chr1_3:12505069-12505088 Msa0104560:CDS 45.0%
!! TTCACTGTTGCTTTGTCTGG+TGG - chr1_3:12505142-12505161 Msa0104560:CDS 45.0%
CTTGGAGAGAAATCCATCAC+TGG + chr1_3:12505527-12505546 Msa0104550:intron 45.0%
!! CAGTGATGGATTTCTCTCCA+AGG - chr1_3:12505525-12505544 Msa0104560:intron 45.0%
GGCACCTGACAAAGGAATAA+TGG + chr1_3:12505897-12505916 Msa0104550:intron 45.0%
ATGGCATAAACGTTGAGAGG+AGG + chr1_3:12505924-12505943 Msa0104550:intron 45.0%
AGGTGGTAGGGAATCATCAA+TGG + chr1_3:12505943-12505962 Msa0104550:intron 45.0%
TAAACATCAGCTGCTCCATC+AGG + chr1_3:12505963-12505982 Msa0104550:intron 45.0%
TGATCTCATGCTCCTAGGTA+TGG - chr1_3:12506052-12506071 Msa0104560:CDS 45.0%
GATCTCATGCTCCTAGGTAT+GGG - chr1_3:12506053-12506072 Msa0104560:CDS 45.0%
AGTCACCACCATTGCAACAT+TGG + chr1_3:12506219-12506238 Msa0104550:three_prime_UTR 45.0%
! AATGTTGCAATGGTGGTGAC+TGG - chr1_3:12506218-12506237 Msa0104560:CDS 45.0%
CTCAAGTGCAGTGTAAACTG+CGG + chr1_3:12506258-12506277 Msa0104550:three_prime_UTR 45.0%
GGCATCACTTAAGTGGGAAA+AGG - chr1_3:12505018-12505037 Msa0104560:CDS 45.0%
! GGAACATCTTGCTGTGTCAT+TGG - chr1_3:12505069-12505088 Msa0104560:CDS 45.0%
!! TTCACTGTTGCTTTGTCTGG+TGG - chr1_3:12505142-12505161 Msa0104560:CDS 45.0%
CTTGGAGAGAAATCCATCAC+TGG + chr1_3:12505527-12505546 Msa0104550:intron 45.0%
!! CAGTGATGGATTTCTCTCCA+AGG - chr1_3:12505525-12505544 Msa0104560:intron 45.0%
GGCACCTGACAAAGGAATAA+TGG + chr1_3:12505897-12505916 Msa0104550:intron 45.0%
ATGGCATAAACGTTGAGAGG+AGG + chr1_3:12505924-12505943 Msa0104550:intron 45.0%
AGGTGGTAGGGAATCATCAA+TGG + chr1_3:12505943-12505962 Msa0104550:intron 45.0%
TAAACATCAGCTGCTCCATC+AGG + chr1_3:12505963-12505982 Msa0104550:intron 45.0%
TGATCTCATGCTCCTAGGTA+TGG - chr1_3:12506052-12506071 Msa0104560:CDS 45.0%
GATCTCATGCTCCTAGGTAT+GGG - chr1_3:12506053-12506072 Msa0104560:CDS 45.0%
AGTCACCACCATTGCAACAT+TGG + chr1_3:12506219-12506238 Msa0104550:three_prime_UTR 45.0%
! AATGTTGCAATGGTGGTGAC+TGG - chr1_3:12506218-12506237 Msa0104560:CDS 45.0%
CTCAAGTGCAGTGTAAACTG+CGG + chr1_3:12506258-12506277 Msa0104550:three_prime_UTR 45.0%
GAGGAATTGGCACCTGACAA+AGG + chr1_3:12505905-12505924 Msa0104550:intron 50.0%
GATGATTCCCTACCACCTGA+TGG - chr1_3:12505945-12505964 Msa0104560:intron 50.0%
! CTTGGAGAAGAGCATGACCT+GGG + chr1_3:12506106-12506125 Msa0104550:three_prime_UTR 50.0%
! TCTTGGAGAAGAGCATGACC+TGG + chr1_3:12506107-12506126 Msa0104550:three_prime_UTR 50.0%
AGGTCATGCTCTTCTCCAAG+AGG - chr1_3:12506106-12506125 Msa0104560:CDS 50.0%
!!! GTGGTTTTGGTGAGTCAGTG+AGG + chr1_3:12506151-12506170 Msa0104550:three_prime_UTR 50.0%
CGCAGTTTACACTGCACTTG+AGG - chr1_3:12506256-12506275 Msa0104560:CDS 50.0%
GAGGAATTGGCACCTGACAA+AGG + chr1_3:12505905-12505924 Msa0104550:intron 50.0%
GATGATTCCCTACCACCTGA+TGG - chr1_3:12505945-12505964 Msa0104560:intron 50.0%
! CTTGGAGAAGAGCATGACCT+GGG + chr1_3:12506106-12506125 Msa0104550:three_prime_UTR 50.0%
! TCTTGGAGAAGAGCATGACC+TGG + chr1_3:12506107-12506126 Msa0104550:three_prime_UTR 50.0%
AGGTCATGCTCTTCTCCAAG+AGG - chr1_3:12506106-12506125 Msa0104560:CDS 50.0%
!!! GTGGTTTTGGTGAGTCAGTG+AGG + chr1_3:12506151-12506170 Msa0104550:three_prime_UTR 50.0%
CGCAGTTTACACTGCACTTG+AGG - chr1_3:12506256-12506275 Msa0104560:CDS 50.0%
AGCTGCTCCATCAGGTGGTA+GGG + chr1_3:12505955-12505974 Msa0104550:intron 55.0%
ACATCAGCTGCTCCATCAGG+TGG + chr1_3:12505960-12505979 Msa0104550:intron 55.0%
CTCCTAGGTATGGGTCCAGA+TGG - chr1_3:12506062-12506081 Msa0104560:CDS 55.0%
GTCCATCTGGACCCATACCT+AGG + chr1_3:12506067-12506086 Msa0104550:three_prime_UTR 55.0%
AGCTGCTCCATCAGGTGGTA+GGG + chr1_3:12505955-12505974 Msa0104550:intron 55.0%
ACATCAGCTGCTCCATCAGG+TGG + chr1_3:12505960-12505979 Msa0104550:intron 55.0%
CTCCTAGGTATGGGTCCAGA+TGG - chr1_3:12506062-12506081 Msa0104560:CDS 55.0%
GTCCATCTGGACCCATACCT+AGG + chr1_3:12506067-12506086 Msa0104550:three_prime_UTR 55.0%
CAGCTGCTCCATCAGGTGGT+AGG + chr1_3:12505956-12505975 Msa0104550:intron 60.0%
! TGCAATGGTGGTGACTGGCG+CGG - chr1_3:12506223-12506242 Msa0104560:CDS 60.0%
CAGCTGCTCCATCAGGTGGT+AGG + chr1_3:12505956-12505975 Msa0104550:intron 60.0%
! TGCAATGGTGGTGACTGGCG+CGG - chr1_3:12506223-12506242 Msa0104560:CDS 60.0%
! GCAATGGTGGTGACTGGCGC+GGG - chr1_3:12506224-12506243 Msa0104560:CDS 65.0%
! GCAATGGTGGTGACTGGCGC+GGG - chr1_3:12506224-12506243 Msa0104560:CDS 65.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 12504833 12506385 12504833 ID=Msa0104560;Name=Msa0104560
chr1_3 mRNA 12504833 12506385 12504833 ID=Msa0104560-mRNA-1;Parent=Msa0104560;Name=Msa0104560-mRNA-1;_AED=0.32;_eAED=0.32;_QI=0|0|0|1|1|1|3|0|320
chr1_3 exon 12506033 12506385 12506033 ID=Msa0104560-mRNA-1:exon:5010;Parent=Msa0104560-mRNA-1
chr1_3 exon 12505672 12505807 12505672 ID=Msa0104560-mRNA-1:exon:5009;Parent=Msa0104560-mRNA-1
chr1_3 exon 12504833 12505306 12504833 ID=Msa0104560-mRNA-1:exon:5008;Parent=Msa0104560-mRNA-1
chr1_3 CDS 12506033 12506385 12506033 ID=Msa0104560-mRNA-1:cds;Parent=Msa0104560-mRNA-1
chr1_3 CDS 12505672 12505807 12505672 ID=Msa0104560-mRNA-1:cds;Parent=Msa0104560-mRNA-1
chr1_3 CDS 12504833 12505306 12504833 ID=Msa0104560-mRNA-1:cds;Parent=Msa0104560-mRNA-1
Gene Sequence

>Msa0104560

ATGGCCTTCTCAACAACTTTGACTAATTCATGCACCCTCCAAAGTAGCTTATTTTGTTCACAAAAATCAAATCCACTATTACTAAAGAAATCAATTTTACCAACCCAAATTGGGAAAAACATTGTTTTTCAACCTCTAAGGCATAATAAAGTGTTTTCACCAAATAGATGTGTCGGAAAAATAGAGGCATCACTTAAGTGGGAAAAGGGTTATAAGAATGTTGAGGTTTTCAGTAAGGAACATCTTGCTGTGTCATTGGCTTATGATGTTGCTCAACTTTCTAACAAATTCATCAAAGAAAGAGGTTATTTCACTGTTGCTTTGTCTGGTGGATCATTGATCAAGTACCTAAGGAAATTGGTTGAGTCTCCATATGCTGAAACCATAGATTGGTCAAAATGGCATGTTTTCTGGGTTGATGAGAGAGTTGTCCCAAAAGATAACTTAGAAAGTAATTATAAGCTTGCCAGTGATGGATTTCTCTCCAAGGTGCCAATTCCTCCTCTCAACGTTTATGCCATTGATGATTCCCTACCACCTGATGGAGCAGCTGATGTTTATGAAACAACCCTAAGACGTTTGGTTAAAAGCAATGTGATATCTACATCAACCAATGAATTACCAAAATTTGATCTCATGCTCCTAGGTATGGGTCCAGATGGACATGTTGCATCTTTATTCCCAGGTCATGCTCTTCTCCAAGAGGATCAGAAATGGGTTACTTTCCTCACTGACTCACCAAAACCACCATCAGAGAGAATTACTTTCACTTTTCCAGTGATCAATGCTTCTTCCAATGTTGCAATGGTGGTGACTGGCGCGGGTAAATCGACCGCAGTTTACACTGCACTTGAGGATGAGAAGAAAACTGTCATGTTGCCTGTTGAAAAGGTTTCACCTCTTGAAGGGGAATTGAAATGGTACTTAGACAAAGGTGCTGCTTCAAAACTGTTTAAGGAGTAA
Protein sequence

>Msa0104560

MAFSTTLTNSCTLQSSLFCSQKSNPLLLKKSILPTQIGKNIVFQPLRHNKVFSPNRCVGKIEASLKWEKGYKNVEVFSKEHLAVSLAYDVAQLSNKFIKERGYFTVALSGGSLIKYLRKLVESPYAETIDWSKWHVFWVDERVVPKDNLESNYKLASDGFLSKVPIPPLNVYAIDDSLPPDGAADVYETTLRRLVKSNVISTSTNELPKFDLMLLGMGPDGHVASLFPGHALLQEDQKWVTFLTDSPKPPSERITFTFPVINASSNVAMVVTGAGKSTAVYTALEDEKKTVMLPVEKVSPLEGELKWYLDKGAASKLFKE*