AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0070470

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0070470 MtrunA17_Chr1g0160181 75.490 102 23 1 66 165 97 198 2.44e-46 155
Msa0070470 MtrunA17_Chr1g0177221 71.569 102 26 2 65 165 96 195 4.10e-43 144
Msa0070470 MtrunA17_Chr3g0114701 57.843 102 41 1 66 165 88 189 2.00e-30 114
Msa0070470 MtrunA17_Chr3g0114731 53.000 100 45 1 66 165 97 194 1.65e-24 98.2
Msa0070470 MtrunA17_Chr3g0114711 49.000 100 51 0 66 165 93 192 6.77e-24 96.3
Msa0070470 MtrunA17_Chr1g0172881 57.647 85 31 2 80 162 6 87 1.07e-22 88.6
Msa0070470 MtrunA17_Chr1g0160171 38.298 94 58 0 71 164 100 193 1.08e-17 79.0
Msa0070470 MtrunA17_Chr7g0255561 33.010 103 65 1 66 164 93 195 1.81e-16 75.9
Msa0070470 MtrunA17_Chr7g0255581 33.010 103 65 1 66 164 93 195 1.81e-16 75.9
Msa0070470 MtrunA17_Chr7g0227921 36.364 99 62 1 66 164 90 187 2.52e-16 75.1
Msa0070470 MtrunA17_Chr3g0114691 38.542 96 57 1 71 164 104 199 2.77e-16 75.1
Msa0070470 MtrunA17_Chr5g0413961 49.275 69 35 0 66 134 92 160 9.64e-16 73.6
Msa0070470 MtrunA17_Chr8g0354121 33.962 106 62 2 66 165 92 195 4.23e-15 72.0
Msa0070470 MtrunA17_Chr1g0182291 49.296 71 36 0 66 136 94 164 5.27e-15 71.2
Msa0070470 MtrunA17_Chr1g0150151 35.577 104 59 2 65 163 90 190 1.26e-14 70.5
Msa0070470 MtrunA17_Chr3g0081511 35.000 100 64 1 66 164 96 195 3.44e-14 69.3
Msa0070470 MtrunA17_Chr6g0451201 37.000 100 60 2 65 164 88 184 3.58e-14 68.9
Msa0070470 MtrunA17_Chr7g0263101 30.714 140 86 2 24 152 42 181 5.82e-14 68.6
Msa0070470 MtrunA17_Chr4g0059311 34.375 96 63 0 70 165 95 190 1.38e-13 67.4
Msa0070470 MtrunA17_Chr3g0126101 36.634 101 60 2 66 165 84 181 2.14e-13 66.6
Msa0070470 MtrunA17_Chr6g0451261 32.323 99 67 0 66 164 90 188 7.57e-13 65.5
Msa0070470 MtrunA17_Chr3g0081421 32.710 107 60 2 66 164 96 198 3.08e-12 63.5
Msa0070470 MtrunA17_Chr5g0413951 37.634 93 56 2 72 163 92 183 6.94e-12 62.4
Msa0070470 MtrunA17_Chr5g0408001 30.476 105 67 2 64 164 87 189 8.82e-12 62.4
Msa0070470 MtrunA17_Chr6g0451211 37.681 69 43 0 66 134 93 161 1.97e-11 61.2
Msa0070470 MtrunA17_Chr1g0164831 34.951 103 62 3 66 165 80 180 2.17e-11 61.2
Msa0070470 MtrunA17_Chr5g0413921 35.922 103 64 2 62 163 82 183 3.75e-11 60.5
Msa0070470 MtrunA17_Chr8g0334761 32.000 100 68 0 66 165 90 189 3.88e-11 60.5
Msa0070470 MtrunA17_Chr1g0155761 27.778 144 83 2 43 165 74 217 5.20e-11 60.1
Msa0070470 MtrunA17_Chr5g0413931 38.710 93 55 2 72 163 91 182 6.03e-11 59.7
Msa0070470 MtrunA17_Chr4g0050891 33.036 112 72 2 40 148 63 174 6.44e-11 59.7
Msa0070470 MtrunA17_Chr3g0142131 29.204 113 67 1 66 165 99 211 7.72e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0070470 AT4G08290.2 44.554 101 54 1 66 164 91 191 6.05e-21 87.4
Msa0070470 AT1G44800.1 37.374 99 62 0 66 164 89 187 2.17e-20 87.0
Msa0070470 AT4G08290.1 44.554 101 54 1 66 164 91 191 2.29e-20 87.0
Msa0070470 AT1G01070.2 41.176 102 58 2 66 165 47 148 8.40e-17 76.6
Msa0070470 AT1G01070.1 41.176 102 58 2 66 165 94 195 1.60e-16 76.3
Msa0070470 AT4G08300.1 36.275 102 62 1 66 164 89 190 7.08e-16 74.3
Msa0070470 AT1G21890.1 45.833 72 39 0 66 137 89 160 9.07e-16 73.9
Msa0070470 AT1G21890.2 45.833 72 39 0 66 137 89 160 1.18e-15 73.6
Msa0070470 AT1G11450.1 36.634 101 62 2 66 165 2 101 2.69e-15 71.6
Msa0070470 AT2G39510.1 39.080 87 53 0 66 152 86 172 4.96e-15 72.0
Msa0070470 AT1G11450.2 36.634 101 62 2 66 165 43 142 5.42e-15 71.2
Msa0070470 AT1G11450.5 36.634 101 62 2 66 165 50 149 6.09e-15 71.2
Msa0070470 AT1G11450.4 36.634 101 62 2 66 165 94 193 7.42e-15 71.2
Msa0070470 AT1G11450.3 36.634 101 62 2 66 165 94 193 9.46e-15 70.9
Msa0070470 AT2G40900.1 42.105 76 44 0 66 141 89 164 1.09e-14 71.2
Msa0070470 AT1G11460.1 35.644 101 64 1 66 165 94 194 1.75e-14 70.1
Msa0070470 AT5G07050.1 41.667 72 42 0 66 137 96 167 3.34e-14 69.7
Msa0070470 AT3G28070.2 38.144 97 56 2 70 163 7 102 1.98e-13 66.6
Msa0070470 AT3G28070.3 38.144 97 56 2 70 163 99 194 3.95e-13 66.2
Msa0070470 AT3G28100.2 35.238 105 66 1 70 172 96 200 4.04e-13 65.9
Msa0070470 AT3G28070.1 38.144 97 56 2 70 163 99 194 4.29e-13 66.2
Msa0070470 AT2G37460.1 43.056 72 41 0 66 137 93 164 5.08e-13 66.2
Msa0070470 AT5G13670.1 34.234 111 64 2 66 167 86 196 8.27e-13 65.5
Msa0070470 AT3G28100.3 35.238 105 66 1 70 172 96 200 9.62e-13 64.7
Msa0070470 AT4G15540.1 44.286 70 39 0 72 141 101 170 9.89e-13 65.5
Msa0070470 AT4G15540.2 44.286 70 39 0 72 141 101 170 9.89e-13 65.5
Msa0070470 AT4G01440.4 32.727 110 58 3 66 167 88 189 1.77e-12 63.9
Msa0070470 AT3G56620.1 32.353 102 61 2 66 165 89 184 1.99e-12 64.7
Msa0070470 AT5G40240.2 35.714 98 56 2 72 164 118 213 2.17e-12 64.3
Msa0070470 AT5G40240.1 35.714 98 56 2 72 164 104 199 2.19e-12 64.3
Msa0070470 AT5G40240.3 35.714 98 56 2 72 164 104 199 2.19e-12 64.3
Msa0070470 AT4G01440.3 33.000 100 65 2 66 164 88 186 2.28e-12 64.3
Msa0070470 AT3G28130.6 46.154 65 35 0 70 134 95 159 2.48e-12 63.2
Msa0070470 AT4G01450.3 44.186 86 41 3 66 145 88 172 2.63e-12 63.2
Msa0070470 AT3G28100.1 35.238 105 66 1 70 172 96 200 2.71e-12 63.9
Msa0070470 AT4G01440.1 33.000 100 65 2 66 164 88 186 2.75e-12 63.9
Msa0070470 AT4G01450.4 44.186 86 41 3 66 145 88 172 3.03e-12 63.2
Msa0070470 AT1G09380.1 31.579 133 88 2 23 152 40 172 3.43e-12 63.9
Msa0070470 AT3G28130.4 46.154 65 35 0 70 134 95 159 4.78e-12 62.8
Msa0070470 AT4G01450.1 44.186 86 41 3 66 145 88 172 5.78e-12 63.2
Msa0070470 AT4G01450.2 44.186 86 41 3 66 145 88 172 6.31e-12 63.2
Msa0070470 AT3G28130.5 46.154 65 35 0 70 134 9 73 7.96e-12 62.4
Msa0070470 AT3G28130.1 46.154 65 35 0 70 134 9 73 7.96e-12 62.4
Msa0070470 AT5G40230.1 46.032 63 34 0 72 134 105 167 8.85e-12 62.8
Msa0070470 AT3G28050.1 31.915 94 60 1 70 163 94 183 9.60e-12 62.4
Msa0070470 AT3G28130.2 46.154 65 35 0 70 134 95 159 1.06e-11 62.4
Msa0070470 AT3G45870.9 30.303 99 69 0 64 162 88 186 1.22e-11 62.0
Msa0070470 AT4G01430.1 37.500 104 60 3 66 165 87 189 2.13e-11 61.6
Msa0070470 AT3G28080.4 36.842 95 58 1 71 163 8 102 2.32e-11 60.5
Msa0070470 AT4G01430.2 37.500 104 60 3 66 165 87 189 2.66e-11 61.2
Msa0070470 AT3G28080.5 36.842 95 58 1 71 163 8 102 3.04e-11 60.5
Msa0070470 AT3G28080.7 36.842 95 58 1 71 163 8 102 3.04e-11 60.5
Msa0070470 AT3G28080.8 36.842 95 58 1 71 163 8 102 3.04e-11 60.5
Msa0070470 AT3G28080.6 36.842 95 58 1 71 163 8 102 3.04e-11 60.5
Msa0070470 AT3G45870.8 30.303 99 69 0 64 162 88 186 3.37e-11 60.8
Msa0070470 AT3G45870.1 30.303 99 69 0 64 162 88 186 4.11e-11 60.8
Msa0070470 AT3G28080.1 36.842 95 58 1 71 163 97 191 5.19e-11 60.5
Msa0070470 AT3G28080.3 36.842 95 58 1 71 163 97 191 5.55e-11 60.1
Msa0070470 AT3G28080.2 36.842 95 58 1 71 163 89 183 5.89e-11 60.1
Msa0070470 AT4G19185.1 31.000 100 67 1 64 161 97 196 8.10e-11 60.1

Find 66 sgRNAs with CRISPR-Local

Find 215 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAACAATTTCTTATAATA+AGG 0.172014 1_2:-41000502 None:intergenic
GCCACTTCTTCTCTATATTT+AGG 0.189515 1_2:+40998801 Msa0070470:five_prime_UTR
ATTATTAACTTCATTCAATA+AGG 0.195450 1_2:-40998339 None:intergenic
TGGTCAATGCTTCTCTTTCT+TGG 0.207912 1_2:+40999977 Msa0070470:CDS
ACAAATGTCCTTAGTGCTTT+TGG 0.229809 1_2:-40998617 None:intergenic
GGTCAATGCTTCTCTTTCTT+GGG 0.242702 1_2:+40999978 Msa0070470:CDS
AGGAATCTAATTTCTAATTC+TGG 0.251850 1_2:+40998530 Msa0070470:five_prime_UTR
TCACAATCTTCATCTAAATA+AGG 0.269539 1_2:+40998506 Msa0070470:five_prime_UTR
AGCATTTGGAAAATCGATTT+AGG 0.277158 1_2:+41000676 Msa0070470:three_prime_UTR
AATCTAACGTCAGCATCTTT+TGG 0.286071 1_2:+41000005 Msa0070470:CDS
AACTTTCAAGTGATAGATAA+TGG 0.317263 1_2:+40998365 Msa0070470:five_prime_UTR
AAACAGACCTGTGAACAATT+TGG 0.334036 1_2:-40998668 None:intergenic
TAAGAATGACTACAAGCATT+TGG 0.368481 1_2:+41000662 Msa0070470:three_prime_UTR
TATCTTCAAAATATTGTGAT+TGG 0.369162 1_2:-40998768 None:intergenic
TTATTAACTTCATTCAATAA+GGG 0.410100 1_2:-40998338 None:intergenic
ATTGGAACGTATGAAGATAA+AGG 0.416123 1_2:+41000306 Msa0070470:CDS
TTGAATTACAACACAACCTC+TGG 0.424153 1_2:+40998742 Msa0070470:five_prime_UTR
TTTATGTACATCAGCATTGT+TGG 0.425034 1_2:+40999943 Msa0070470:intron
GCAAGTATAAAGGTCATAGA+AGG 0.429411 1_2:-41000050 None:intergenic
GGACCTATAGTCAGCCTTGA+AGG 0.444809 1_2:+41000355 Msa0070470:CDS
AGTCTAAGTTCCTCCTACCT+TGG 0.446256 1_2:-41000717 None:intergenic
GACTTAGCAGCTTGAAGAAA+GGG 0.457652 1_2:-41000406 None:intergenic
CGTATGAAGATAAAGGAGAT+TGG 0.458516 1_2:+41000313 Msa0070470:CDS
ACACGACGACAACAGCATTC+CGG 0.465397 1_2:-40998919 None:intergenic
GTCAATGCTTCTCTTTCTTG+GGG 0.468477 1_2:+40999979 Msa0070470:CDS
TCATTAATAGTGCTCCTTCA+AGG 0.469872 1_2:-41000369 None:intergenic
TGTAGAAAGTGATAATGAAC+GGG 0.492193 1_2:-40998834 None:intergenic
GCTCCTTCAAGGCTGACTAT+AGG 0.494342 1_2:-41000358 None:intergenic
CATTGTACCAAATTGTTCAC+AGG 0.496006 1_2:+40998661 Msa0070470:five_prime_UTR
TTGTGAAATAAGAGAAAAGA+TGG 0.509291 1_2:-40998489 None:intergenic
AATAAACAGTCTACATTCAA+AGG 0.515333 1_2:+40999058 Msa0070470:CDS
AGTGGTTCACATTGGCTCGT+TGG 0.524320 1_2:+41000469 Msa0070470:CDS
CATTCCGGTGCGGTAGTTGA+CGG 0.525239 1_2:-40998904 None:intergenic
GGAAGTCAAGTGAAAGCGAT+TGG 0.526618 1_2:+41000334 Msa0070470:CDS
CACTAAGGACATTTGTTCAC+GGG 0.527732 1_2:+40998624 Msa0070470:five_prime_UTR
GAAGGATTGTAGTTTATGAA+AGG 0.536578 1_2:-40998861 None:intergenic
TCCTAAATATAGAGAAGAAG+TGG 0.540737 1_2:-40998802 None:intergenic
TGACTTAGCAGCTTGAAGAA+AGG 0.541265 1_2:-41000407 None:intergenic
ATGTACATCAGCATTGTTGG+AGG 0.542794 1_2:+40999946 Msa0070470:intron
GCAGACGTTGGTGCTTGCGA+AGG 0.543362 1_2:-40998879 None:intergenic
AGTAGTTGAGCCAAGGTAGG+AGG 0.548081 1_2:+41000707 Msa0070470:three_prime_UTR
CATTGTTGGAGGAGTGGTGG+TGG 0.549329 1_2:+40999957 Msa0070470:CDS
TGCCAAACCCAAAAGCACTA+AGG 0.555424 1_2:+40998609 Msa0070470:five_prime_UTR
GAATTTGAGTAGTTGAGCCA+AGG 0.558092 1_2:+41000700 Msa0070470:three_prime_UTR
TATAAAGGTCATAGAAGGAA+CGG 0.579409 1_2:-41000045 None:intergenic
TTGAGTAGTTGAGCCAAGGT+AGG 0.581131 1_2:+41000704 Msa0070470:three_prime_UTR
GCACTAAGGACATTTGTTCA+CGG 0.581319 1_2:+40998623 Msa0070470:five_prime_UTR
TAGTTGACGGCGGCAGACGT+TGG 0.583127 1_2:-40998891 None:intergenic
AATCTTCATCTAAATAAGGA+AGG 0.583600 1_2:+40998510 Msa0070470:five_prime_UTR
TGCACAACATGATAAGAAGA+TGG 0.587404 1_2:+40998452 Msa0070470:five_prime_UTR
TATGCACAATTGCACATGGA+TGG 0.592947 1_2:+40998581 Msa0070470:five_prime_UTR
GCCGCCGTCAACTACCGCAC+CGG 0.593698 1_2:+40998900 Msa0070470:CDS
TTGTAGAAAGTGATAATGAA+CGG 0.596568 1_2:-40998835 None:intergenic
CCAGCAAGGATTATGAAATG+AGG 0.604111 1_2:+40999015 Msa0070470:intron
ACGAGCCAATGTGAACCACT+AGG 0.604666 1_2:-41000466 None:intergenic
CTGTGAACAATTTGGTACAA+TGG 0.605661 1_2:-40998660 None:intergenic
CATCAGCATTGTTGGAGGAG+TGG 0.613229 1_2:+40999951 Msa0070470:intron
CGAGCCAATGTGAACCACTA+GGG 0.623499 1_2:-41000465 None:intergenic
ACGCTATGCACAATTGCACA+TGG 0.634860 1_2:+40998577 Msa0070470:five_prime_UTR
CAGCATTGTTGGAGGAGTGG+TGG 0.646899 1_2:+40999954 Msa0070470:intron
ATATTGTGATTGGTATCCAG+AGG 0.656570 1_2:-40998758 None:intergenic
ATGACCCTAGTGGTTCACAT+TGG 0.672223 1_2:+41000461 Msa0070470:CDS
TCCGGTGCGGTAGTTGACGG+CGG 0.677860 1_2:-40998901 None:intergenic
CTATTAATGATACTCTACAA+AGG 0.689649 1_2:+41000382 Msa0070470:CDS
ACGACAACAGCATTCCGGTG+CGG 0.747692 1_2:-40998914 None:intergenic
GAAAATGTGAATGACCCTAG+TGG 0.771669 1_2:+41000451 Msa0070470:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAATAAAATATAAGTAAAA+CGG - chr1_2:40999273-40999292 None:intergenic 10.0%
!!! ATCATAATGATATTTTTTTA+AGG - chr1_2:40999318-40999337 None:intergenic 10.0%
!! TCAAATATTAGATTTAAATT+TGG + chr1_2:40999409-40999428 Msa0070470:intron 10.0%
!! TTCTTATATTTATCAAAATA+GGG - chr1_2:40999444-40999463 None:intergenic 10.0%
!! TTTCTTATATTTATCAAAAT+AGG - chr1_2:40999445-40999464 None:intergenic 10.0%
!! AGAATAAAATATAAGTAAAA+CGG - chr1_2:40999273-40999292 None:intergenic 10.0%
!!! ATCATAATGATATTTTTTTA+AGG - chr1_2:40999318-40999337 None:intergenic 10.0%
!! TCAAATATTAGATTTAAATT+TGG + chr1_2:40999409-40999428 Msa0070470:intron 10.0%
!! TTCTTATATTTATCAAAATA+GGG - chr1_2:40999444-40999463 None:intergenic 10.0%
!! TTTCTTATATTTATCAAAAT+AGG - chr1_2:40999445-40999464 None:intergenic 10.0%
!! TTATTAACTTCATTCAATAA+GGG - chr1_2:40998341-40998360 None:intergenic 15.0%
!! ATTATTAACTTCATTCAATA+AGG - chr1_2:40998342-40998361 None:intergenic 15.0%
!!! ACTTATATTTTATTCTCATA+AGG + chr1_2:40999277-40999296 Msa0070470:intron 15.0%
!! AATCTAATATTTGACAAATT+AGG - chr1_2:40999403-40999422 None:intergenic 15.0%
!! TATATATTATGTTGAAAGTT+CGG - chr1_2:40999625-40999644 None:intergenic 15.0%
!!! TCTACATTAAATATTTTTTC+CGG + chr1_2:40999697-40999716 Msa0070470:intron 15.0%
!!! TCATTAAAATCAAACATTTT+TGG + chr1_2:40999882-40999901 Msa0070470:intron 15.0%
!! AAAAATTAATGCAAGTATAA+AGG - chr1_2:41000063-41000082 None:intergenic 15.0%
!!! TTATACTTGCATTAATTTTT+CGG + chr1_2:41000063-41000082 Msa0070470:CDS 15.0%
!! CAAAACAATTTCTTATAATA+AGG - chr1_2:41000505-41000524 None:intergenic 15.0%
!! TTATTAACTTCATTCAATAA+GGG - chr1_2:40998341-40998360 None:intergenic 15.0%
!! ATTATTAACTTCATTCAATA+AGG - chr1_2:40998342-40998361 None:intergenic 15.0%
!!! ACTTATATTTTATTCTCATA+AGG + chr1_2:40999277-40999296 Msa0070470:intron 15.0%
!! AATCTAATATTTGACAAATT+AGG - chr1_2:40999403-40999422 None:intergenic 15.0%
!! TATATATTATGTTGAAAGTT+CGG - chr1_2:40999625-40999644 None:intergenic 15.0%
!!! TCTACATTAAATATTTTTTC+CGG + chr1_2:40999697-40999716 Msa0070470:intron 15.0%
!!! TCATTAAAATCAAACATTTT+TGG + chr1_2:40999882-40999901 Msa0070470:intron 15.0%
!! AAAAATTAATGCAAGTATAA+AGG - chr1_2:41000063-41000082 None:intergenic 15.0%
!!! TTATACTTGCATTAATTTTT+CGG + chr1_2:41000063-41000082 Msa0070470:CDS 15.0%
!! CAAAACAATTTCTTATAATA+AGG - chr1_2:41000505-41000524 None:intergenic 15.0%
!! TATCTTCAAAATATTGTGAT+TGG - chr1_2:40998771-40998790 None:intergenic 20.0%
!! TAGATTTAAATTTGGCAAAT+TGG + chr1_2:40999417-40999436 Msa0070470:intron 20.0%
!! TATCTTCAAAATATTGTGAT+TGG - chr1_2:40998771-40998790 None:intergenic 20.0%
!! TAGATTTAAATTTGGCAAAT+TGG + chr1_2:40999417-40999436 Msa0070470:intron 20.0%
!! AACTTTCAAGTGATAGATAA+TGG + chr1_2:40998365-40998384 Msa0070470:five_prime_UTR 25.0%
! TTGTGAAATAAGAGAAAAGA+TGG - chr1_2:40998492-40998511 None:intergenic 25.0%
! TCACAATCTTCATCTAAATA+AGG + chr1_2:40998506-40998525 Msa0070470:five_prime_UTR 25.0%
! AATCTTCATCTAAATAAGGA+AGG + chr1_2:40998510-40998529 Msa0070470:five_prime_UTR 25.0%
! AGGAATCTAATTTCTAATTC+TGG + chr1_2:40998530-40998549 Msa0070470:five_prime_UTR 25.0%
!! TTGTAGAAAGTGATAATGAA+CGG - chr1_2:40998838-40998857 None:intergenic 25.0%
! AATAAACAGTCTACATTCAA+AGG + chr1_2:40999058-40999077 Msa0070470:CDS 25.0%
! TTTATCATATTTGTGCAGAT+TGG + chr1_2:40999470-40999489 Msa0070470:intron 25.0%
!! TTGATCACATTAATCTTTTC+CGG - chr1_2:40999719-40999738 None:intergenic 25.0%
! TTAAGAGATAGCATTATACA+AGG + chr1_2:40999779-40999798 Msa0070470:intron 25.0%
! GTACATAAAACAAATCAAAG+TGG - chr1_2:40999932-40999951 None:intergenic 25.0%
!!! GTATCTAAAACACTTTTAGT+CGG - chr1_2:41000231-41000250 None:intergenic 25.0%
!!! CATTTTATTTTTGAGCAGAT+TGG + chr1_2:41000288-41000307 Msa0070470:intron 25.0%
! CTATTAATGATACTCTACAA+AGG + chr1_2:41000382-41000401 Msa0070470:CDS 25.0%
!! ATATGCATTTCATTTTCACT+CGG - chr1_2:41000605-41000624 None:intergenic 25.0%
!! AACTTTCAAGTGATAGATAA+TGG + chr1_2:40998365-40998384 Msa0070470:five_prime_UTR 25.0%
! TTGTGAAATAAGAGAAAAGA+TGG - chr1_2:40998492-40998511 None:intergenic 25.0%
! TCACAATCTTCATCTAAATA+AGG + chr1_2:40998506-40998525 Msa0070470:five_prime_UTR 25.0%
! AATCTTCATCTAAATAAGGA+AGG + chr1_2:40998510-40998529 Msa0070470:five_prime_UTR 25.0%
! AGGAATCTAATTTCTAATTC+TGG + chr1_2:40998530-40998549 Msa0070470:five_prime_UTR 25.0%
!! TTGTAGAAAGTGATAATGAA+CGG - chr1_2:40998838-40998857 None:intergenic 25.0%
! AATAAACAGTCTACATTCAA+AGG + chr1_2:40999058-40999077 Msa0070470:CDS 25.0%
! TTTATCATATTTGTGCAGAT+TGG + chr1_2:40999470-40999489 Msa0070470:intron 25.0%
!! TTGATCACATTAATCTTTTC+CGG - chr1_2:40999719-40999738 None:intergenic 25.0%
! TTAAGAGATAGCATTATACA+AGG + chr1_2:40999779-40999798 Msa0070470:intron 25.0%
! GTACATAAAACAAATCAAAG+TGG - chr1_2:40999932-40999951 None:intergenic 25.0%
!!! GTATCTAAAACACTTTTAGT+CGG - chr1_2:41000231-41000250 None:intergenic 25.0%
!!! CATTTTATTTTTGAGCAGAT+TGG + chr1_2:41000288-41000307 Msa0070470:intron 25.0%
! CTATTAATGATACTCTACAA+AGG + chr1_2:41000382-41000401 Msa0070470:CDS 25.0%
!! ATATGCATTTCATTTTCACT+CGG - chr1_2:41000605-41000624 None:intergenic 25.0%
TCCTAAATATAGAGAAGAAG+TGG - chr1_2:40998805-40998824 None:intergenic 30.0%
! TGTAGAAAGTGATAATGAAC+GGG - chr1_2:40998837-40998856 None:intergenic 30.0%
GAAGGATTGTAGTTTATGAA+AGG - chr1_2:40998864-40998883 None:intergenic 30.0%
! GCATAAACTACACAATTTTC+TGG - chr1_2:40998956-40998975 None:intergenic 30.0%
!!! AATTGTTTTTTTACCTTGCC+CGG - chr1_2:40999149-40999168 None:intergenic 30.0%
! ATTGGAAGGCATGAAAATTA+AGG + chr1_2:40999488-40999507 Msa0070470:intron 30.0%
! CAACATAAGTCTTTTTGGAT+CGG + chr1_2:40999668-40999687 Msa0070470:intron 30.0%
TTTATGTACATCAGCATTGT+TGG + chr1_2:40999943-40999962 Msa0070470:intron 30.0%
TATAAAGGTCATAGAAGGAA+CGG - chr1_2:41000048-41000067 None:intergenic 30.0%
!! CTTGCATTAATTTTTCGGTA+AGG + chr1_2:41000068-41000087 Msa0070470:intron 30.0%
!! CGTGCTTGTTTCTATTATTA+CGG - chr1_2:41000172-41000191 None:intergenic 30.0%
ATTGGAACGTATGAAGATAA+AGG + chr1_2:41000306-41000325 Msa0070470:CDS 30.0%
! TTTTCACTCGGTTTTCAAAA+TGG - chr1_2:41000593-41000612 None:intergenic 30.0%
TAAGAATGACTACAAGCATT+TGG + chr1_2:41000662-41000681 Msa0070470:three_prime_UTR 30.0%
! AGCATTTGGAAAATCGATTT+AGG + chr1_2:41000676-41000695 Msa0070470:three_prime_UTR 30.0%
TCCTAAATATAGAGAAGAAG+TGG - chr1_2:40998805-40998824 None:intergenic 30.0%
! TGTAGAAAGTGATAATGAAC+GGG - chr1_2:40998837-40998856 None:intergenic 30.0%
GAAGGATTGTAGTTTATGAA+AGG - chr1_2:40998864-40998883 None:intergenic 30.0%
! GCATAAACTACACAATTTTC+TGG - chr1_2:40998956-40998975 None:intergenic 30.0%
!!! AATTGTTTTTTTACCTTGCC+CGG - chr1_2:40999149-40999168 None:intergenic 30.0%
! ATTGGAAGGCATGAAAATTA+AGG + chr1_2:40999488-40999507 Msa0070470:intron 30.0%
! CAACATAAGTCTTTTTGGAT+CGG + chr1_2:40999668-40999687 Msa0070470:intron 30.0%
TTTATGTACATCAGCATTGT+TGG + chr1_2:40999943-40999962 Msa0070470:intron 30.0%
TATAAAGGTCATAGAAGGAA+CGG - chr1_2:41000048-41000067 None:intergenic 30.0%
!! CTTGCATTAATTTTTCGGTA+AGG + chr1_2:41000068-41000087 Msa0070470:intron 30.0%
!! CGTGCTTGTTTCTATTATTA+CGG - chr1_2:41000172-41000191 None:intergenic 30.0%
ATTGGAACGTATGAAGATAA+AGG + chr1_2:41000306-41000325 Msa0070470:CDS 30.0%
! TTTTCACTCGGTTTTCAAAA+TGG - chr1_2:41000593-41000612 None:intergenic 30.0%
TAAGAATGACTACAAGCATT+TGG + chr1_2:41000662-41000681 Msa0070470:three_prime_UTR 30.0%
! AGCATTTGGAAAATCGATTT+AGG + chr1_2:41000676-41000695 Msa0070470:three_prime_UTR 30.0%
TGCACAACATGATAAGAAGA+TGG + chr1_2:40998452-40998471 Msa0070470:five_prime_UTR 35.0%
! CAAATGTCCTTAGTGCTTTT+GGG - chr1_2:40998619-40998638 None:intergenic 35.0%
! ACAAATGTCCTTAGTGCTTT+TGG - chr1_2:40998620-40998639 None:intergenic 35.0%
CTGTGAACAATTTGGTACAA+TGG - chr1_2:40998663-40998682 None:intergenic 35.0%
CATTGTACCAAATTGTTCAC+AGG + chr1_2:40998661-40998680 Msa0070470:five_prime_UTR 35.0%
AAACAGACCTGTGAACAATT+TGG - chr1_2:40998671-40998690 None:intergenic 35.0%
TTGAATTACAACACAACCTC+TGG + chr1_2:40998742-40998761 Msa0070470:five_prime_UTR 35.0%
ATATTGTGATTGGTATCCAG+AGG - chr1_2:40998761-40998780 None:intergenic 35.0%
GCCACTTCTTCTCTATATTT+AGG + chr1_2:40998801-40998820 Msa0070470:five_prime_UTR 35.0%
! TTGATTTGATGTAACCAGCA+AGG + chr1_2:40999001-40999020 Msa0070470:intron 35.0%
CAAAAATGATCCTTCAACTG+TGG + chr1_2:40999097-40999116 Msa0070470:intron 35.0%
CGAAGGATTACTTTAATCTC+CGG + chr1_2:40999127-40999146 Msa0070470:intron 35.0%
GAAGGATTACTTTAATCTCC+GGG + chr1_2:40999128-40999147 Msa0070470:intron 35.0%
TCATATTTGTGCAGATTGGA+AGG + chr1_2:40999474-40999493 Msa0070470:intron 35.0%
GGCATGAAAATTAAGGAGAT+AGG + chr1_2:40999495-40999514 Msa0070470:intron 35.0%
! CGCATCAACATAAGTCTTTT+TGG + chr1_2:40999663-40999682 Msa0070470:intron 35.0%
! AATCTAACGTCAGCATCTTT+TGG + chr1_2:41000005-41000024 Msa0070470:CDS 35.0%
GCAAGTATAAAGGTCATAGA+AGG - chr1_2:41000053-41000072 None:intergenic 35.0%
CGTATGAAGATAAAGGAGAT+TGG + chr1_2:41000313-41000332 Msa0070470:CDS 35.0%
TCATTAATAGTGCTCCTTCA+AGG - chr1_2:41000372-41000391 None:intergenic 35.0%
! TAGGGTCATTCACATTTTCA+GGG - chr1_2:41000450-41000469 None:intergenic 35.0%
TGCACAACATGATAAGAAGA+TGG + chr1_2:40998452-40998471 Msa0070470:five_prime_UTR 35.0%
! CAAATGTCCTTAGTGCTTTT+GGG - chr1_2:40998619-40998638 None:intergenic 35.0%
! ACAAATGTCCTTAGTGCTTT+TGG - chr1_2:40998620-40998639 None:intergenic 35.0%
CTGTGAACAATTTGGTACAA+TGG - chr1_2:40998663-40998682 None:intergenic 35.0%
CATTGTACCAAATTGTTCAC+AGG + chr1_2:40998661-40998680 Msa0070470:five_prime_UTR 35.0%
AAACAGACCTGTGAACAATT+TGG - chr1_2:40998671-40998690 None:intergenic 35.0%
TTGAATTACAACACAACCTC+TGG + chr1_2:40998742-40998761 Msa0070470:five_prime_UTR 35.0%
ATATTGTGATTGGTATCCAG+AGG - chr1_2:40998761-40998780 None:intergenic 35.0%
GCCACTTCTTCTCTATATTT+AGG + chr1_2:40998801-40998820 Msa0070470:five_prime_UTR 35.0%
! TTGATTTGATGTAACCAGCA+AGG + chr1_2:40999001-40999020 Msa0070470:intron 35.0%
CAAAAATGATCCTTCAACTG+TGG + chr1_2:40999097-40999116 Msa0070470:intron 35.0%
CGAAGGATTACTTTAATCTC+CGG + chr1_2:40999127-40999146 Msa0070470:intron 35.0%
GAAGGATTACTTTAATCTCC+GGG + chr1_2:40999128-40999147 Msa0070470:intron 35.0%
TCATATTTGTGCAGATTGGA+AGG + chr1_2:40999474-40999493 Msa0070470:intron 35.0%
GGCATGAAAATTAAGGAGAT+AGG + chr1_2:40999495-40999514 Msa0070470:intron 35.0%
! CGCATCAACATAAGTCTTTT+TGG + chr1_2:40999663-40999682 Msa0070470:intron 35.0%
! AATCTAACGTCAGCATCTTT+TGG + chr1_2:41000005-41000024 Msa0070470:CDS 35.0%
GCAAGTATAAAGGTCATAGA+AGG - chr1_2:41000053-41000072 None:intergenic 35.0%
CGTATGAAGATAAAGGAGAT+TGG + chr1_2:41000313-41000332 Msa0070470:CDS 35.0%
TCATTAATAGTGCTCCTTCA+AGG - chr1_2:41000372-41000391 None:intergenic 35.0%
! TAGGGTCATTCACATTTTCA+GGG - chr1_2:41000450-41000469 None:intergenic 35.0%
TATGCACAATTGCACATGGA+TGG + chr1_2:40998581-40998600 Msa0070470:five_prime_UTR 40.0%
GCACTAAGGACATTTGTTCA+CGG + chr1_2:40998623-40998642 Msa0070470:five_prime_UTR 40.0%
CACTAAGGACATTTGTTCAC+GGG + chr1_2:40998624-40998643 Msa0070470:five_prime_UTR 40.0%
CCTCATTTCATAATCCTTGC+TGG - chr1_2:40999018-40999037 None:intergenic 40.0%
CCAGCAAGGATTATGAAATG+AGG + chr1_2:40999015-40999034 Msa0070470:intron 40.0%
ACAGTCTACATTCAAAGGTG+AGG + chr1_2:40999063-40999082 Msa0070470:intron 40.0%
TCGAATCTTTCCACAGTTGA+AGG - chr1_2:40999110-40999129 None:intergenic 40.0%
TCAACTGTGGAAAGATTCGA+AGG + chr1_2:40999110-40999129 Msa0070470:intron 40.0%
ATTACTTTAATCTCCGGGCA+AGG + chr1_2:40999133-40999152 Msa0070470:intron 40.0%
ATGTACATCAGCATTGTTGG+AGG + chr1_2:40999946-40999965 Msa0070470:intron 40.0%
TGGTCAATGCTTCTCTTTCT+TGG + chr1_2:40999977-40999996 Msa0070470:CDS 40.0%
GGTCAATGCTTCTCTTTCTT+GGG + chr1_2:40999978-40999997 Msa0070470:CDS 40.0%
GTCAATGCTTCTCTTTCTTG+GGG + chr1_2:40999979-40999998 Msa0070470:CDS 40.0%
GACTTAGCAGCTTGAAGAAA+GGG - chr1_2:41000409-41000428 None:intergenic 40.0%
TGACTTAGCAGCTTGAAGAA+AGG - chr1_2:41000410-41000429 None:intergenic 40.0%
! CTAGGGTCATTCACATTTTC+AGG - chr1_2:41000451-41000470 None:intergenic 40.0%
GAAAATGTGAATGACCCTAG+TGG + chr1_2:41000451-41000470 Msa0070470:CDS 40.0%
GAATTTGAGTAGTTGAGCCA+AGG + chr1_2:41000700-41000719 Msa0070470:three_prime_UTR 40.0%
TATGCACAATTGCACATGGA+TGG + chr1_2:40998581-40998600 Msa0070470:five_prime_UTR 40.0%
GCACTAAGGACATTTGTTCA+CGG + chr1_2:40998623-40998642 Msa0070470:five_prime_UTR 40.0%
CACTAAGGACATTTGTTCAC+GGG + chr1_2:40998624-40998643 Msa0070470:five_prime_UTR 40.0%
CCTCATTTCATAATCCTTGC+TGG - chr1_2:40999018-40999037 None:intergenic 40.0%
CCAGCAAGGATTATGAAATG+AGG + chr1_2:40999015-40999034 Msa0070470:intron 40.0%
ACAGTCTACATTCAAAGGTG+AGG + chr1_2:40999063-40999082 Msa0070470:intron 40.0%
TCGAATCTTTCCACAGTTGA+AGG - chr1_2:40999110-40999129 None:intergenic 40.0%
TCAACTGTGGAAAGATTCGA+AGG + chr1_2:40999110-40999129 Msa0070470:intron 40.0%
ATTACTTTAATCTCCGGGCA+AGG + chr1_2:40999133-40999152 Msa0070470:intron 40.0%
ATGTACATCAGCATTGTTGG+AGG + chr1_2:40999946-40999965 Msa0070470:intron 40.0%
TGGTCAATGCTTCTCTTTCT+TGG + chr1_2:40999977-40999996 Msa0070470:CDS 40.0%
GGTCAATGCTTCTCTTTCTT+GGG + chr1_2:40999978-40999997 Msa0070470:CDS 40.0%
GTCAATGCTTCTCTTTCTTG+GGG + chr1_2:40999979-40999998 Msa0070470:CDS 40.0%
GACTTAGCAGCTTGAAGAAA+GGG - chr1_2:41000409-41000428 None:intergenic 40.0%
TGACTTAGCAGCTTGAAGAA+AGG - chr1_2:41000410-41000429 None:intergenic 40.0%
! CTAGGGTCATTCACATTTTC+AGG - chr1_2:41000451-41000470 None:intergenic 40.0%
GAAAATGTGAATGACCCTAG+TGG + chr1_2:41000451-41000470 Msa0070470:CDS 40.0%
GAATTTGAGTAGTTGAGCCA+AGG + chr1_2:41000700-41000719 Msa0070470:three_prime_UTR 40.0%
ACGCTATGCACAATTGCACA+TGG + chr1_2:40998577-40998596 Msa0070470:five_prime_UTR 45.0%
!! TGCCAAACCCAAAAGCACTA+AGG + chr1_2:40998609-40998628 Msa0070470:five_prime_UTR 45.0%
!!! GTCCTTAGTGCTTTTGGGTT+TGG - chr1_2:40998614-40998633 None:intergenic 45.0%
GGAAGTCAAGTGAAAGCGAT+TGG + chr1_2:41000334-41000353 Msa0070470:CDS 45.0%
ATGACCCTAGTGGTTCACAT+TGG + chr1_2:41000461-41000480 Msa0070470:CDS 45.0%
TTGAGTAGTTGAGCCAAGGT+AGG + chr1_2:41000704-41000723 Msa0070470:three_prime_UTR 45.0%
ACGCTATGCACAATTGCACA+TGG + chr1_2:40998577-40998596 Msa0070470:five_prime_UTR 45.0%
!! TGCCAAACCCAAAAGCACTA+AGG + chr1_2:40998609-40998628 Msa0070470:five_prime_UTR 45.0%
!!! GTCCTTAGTGCTTTTGGGTT+TGG - chr1_2:40998614-40998633 None:intergenic 45.0%
GGAAGTCAAGTGAAAGCGAT+TGG + chr1_2:41000334-41000353 Msa0070470:CDS 45.0%
ATGACCCTAGTGGTTCACAT+TGG + chr1_2:41000461-41000480 Msa0070470:CDS 45.0%
!!! AGTGCTTTTGGGTTTGGCAG+TGG - chr1_2:40998608-40998627 None:intergenic 50.0%
ACACGACGACAACAGCATTC+CGG - chr1_2:40998922-40998941 None:intergenic 50.0%
CATCAGCATTGTTGGAGGAG+TGG + chr1_2:40999951-40999970 Msa0070470:intron 50.0%
GGACCTATAGTCAGCCTTGA+AGG + chr1_2:41000355-41000374 Msa0070470:CDS 50.0%
GCTCCTTCAAGGCTGACTAT+AGG - chr1_2:41000361-41000380 None:intergenic 50.0%
CGAGCCAATGTGAACCACTA+GGG - chr1_2:41000468-41000487 None:intergenic 50.0%
ACGAGCCAATGTGAACCACT+AGG - chr1_2:41000469-41000488 None:intergenic 50.0%
!! AGTGGTTCACATTGGCTCGT+TGG + chr1_2:41000469-41000488 Msa0070470:CDS 50.0%
!!! AGTGCTTTTGGGTTTGGCAG+TGG - chr1_2:40998608-40998627 None:intergenic 50.0%
ACACGACGACAACAGCATTC+CGG - chr1_2:40998922-40998941 None:intergenic 50.0%
CATCAGCATTGTTGGAGGAG+TGG + chr1_2:40999951-40999970 Msa0070470:intron 50.0%
GGACCTATAGTCAGCCTTGA+AGG + chr1_2:41000355-41000374 Msa0070470:CDS 50.0%
GCTCCTTCAAGGCTGACTAT+AGG - chr1_2:41000361-41000380 None:intergenic 50.0%
CGAGCCAATGTGAACCACTA+GGG - chr1_2:41000468-41000487 None:intergenic 50.0%
ACGAGCCAATGTGAACCACT+AGG - chr1_2:41000469-41000488 None:intergenic 50.0%
!! AGTGGTTCACATTGGCTCGT+TGG + chr1_2:41000469-41000488 Msa0070470:CDS 50.0%
CATTCCGGTGCGGTAGTTGA+CGG - chr1_2:40998907-40998926 None:intergenic 55.0%
ACGACAACAGCATTCCGGTG+CGG - chr1_2:40998917-40998936 None:intergenic 55.0%
CAGCATTGTTGGAGGAGTGG+TGG + chr1_2:40999954-40999973 Msa0070470:intron 55.0%
CATTGTTGGAGGAGTGGTGG+TGG + chr1_2:40999957-40999976 Msa0070470:CDS 55.0%
CATTCCGGTGCGGTAGTTGA+CGG - chr1_2:40998907-40998926 None:intergenic 55.0%
ACGACAACAGCATTCCGGTG+CGG - chr1_2:40998917-40998936 None:intergenic 55.0%
CAGCATTGTTGGAGGAGTGG+TGG + chr1_2:40999954-40999973 Msa0070470:intron 55.0%
CATTGTTGGAGGAGTGGTGG+TGG + chr1_2:40999957-40999976 Msa0070470:CDS 55.0%
! GCAGACGTTGGTGCTTGCGA+AGG - chr1_2:40998882-40998901 None:intergenic 60.0%
TAGTTGACGGCGGCAGACGT+TGG - chr1_2:40998894-40998913 None:intergenic 60.0%
! GCAGACGTTGGTGCTTGCGA+AGG - chr1_2:40998882-40998901 None:intergenic 60.0%
TAGTTGACGGCGGCAGACGT+TGG - chr1_2:40998894-40998913 None:intergenic 60.0%
TCCGGTGCGGTAGTTGACGG+CGG - chr1_2:40998904-40998923 None:intergenic 65.0%
TCCGGTGCGGTAGTTGACGG+CGG - chr1_2:40998904-40998923 None:intergenic 65.0%
GCCGCCGTCAACTACCGCAC+CGG + chr1_2:40998900-40998919 Msa0070470:CDS 70.0%
GCCGCCGTCAACTACCGCAC+CGG + chr1_2:40998900-40998919 Msa0070470:CDS 70.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 40998332 41000726 40998332 ID=Msa0070470;Name=Msa0070470
chr1_2 mRNA 40998332 41000726 40998332 ID=Msa0070470-mRNA-1;Parent=Msa0070470;Name=Msa0070470-mRNA-1;_AED=0.03;_eAED=0.03;_QI=444|0.75|0.8|1|0.75|0.6|5|201|172
chr1_2 exon 40998332 40998686 40998332 ID=Msa0070470-mRNA-1:exon:12462;Parent=Msa0070470-mRNA-1
chr1_2 exon 40998741 40998939 40998741 ID=Msa0070470-mRNA-1:exon:12463;Parent=Msa0070470-mRNA-1
chr1_2 exon 40999019 40999079 40999019 ID=Msa0070470-mRNA-1:exon:12464;Parent=Msa0070470-mRNA-1
chr1_2 exon 40999957 41000084 40999957 ID=Msa0070470-mRNA-1:exon:12465;Parent=Msa0070470-mRNA-1
chr1_2 exon 41000306 41000726 41000306 ID=Msa0070470-mRNA-1:exon:12466;Parent=Msa0070470-mRNA-1
chr1_2 five_prime_UTR 40998332 40998686 40998332 ID=Msa0070470-mRNA-1:five_prime_utr;Parent=Msa0070470-mRNA-1
chr1_2 five_prime_UTR 40998741 40998829 40998741 ID=Msa0070470-mRNA-1:five_prime_utr;Parent=Msa0070470-mRNA-1
chr1_2 CDS 40998830 40998939 40998830 ID=Msa0070470-mRNA-1:cds;Parent=Msa0070470-mRNA-1
chr1_2 CDS 40999019 40999079 40999019 ID=Msa0070470-mRNA-1:cds;Parent=Msa0070470-mRNA-1
chr1_2 CDS 40999957 41000084 40999957 ID=Msa0070470-mRNA-1:cds;Parent=Msa0070470-mRNA-1
chr1_2 CDS 41000306 41000525 41000306 ID=Msa0070470-mRNA-1:cds;Parent=Msa0070470-mRNA-1
chr1_2 three_prime_UTR 41000526 41000726 41000526 ID=Msa0070470-mRNA-1:three_prime_utr;Parent=Msa0070470-mRNA-1
Gene Sequence

>Msa0070470

ATGACCCGTTCATTATCACTTTCTACAATCACCTTTCATAAACTACAATCCTTCGCAAGCACCAACGTCTGCCGCCGTCAACTACCGCACCGGAATGCTGTTGTCGTCGTCAAGGATTATGAAATGAGGAAGAAGAGAAAACAATGTATAATAAACAGTCTACATTCAAAGCATTGTTGGAGGAGTGGTGGTGGTCAATGCTTCTCTTTCTTGGGGATGAATCTAACGTCAGCATCTTTTGGATCTGCTGTGATGAGCTCCGTTCCTTCTATGACCTTTATACTTGCATTAATTTTTCGATTGGAACGTATGAAGATAAAGGAGATTGGAAGTCAAGTGAAAGCGATTGGACCTATAGTCAGCCTTGAAGGAGCACTATTAATGATACTCTACAAAGGTCCCTTTCTTCAAGCTGCTAAGTCATCAGCACATACTATGTACCCTGAAAATGTGAATGACCCTAGTGGTTCACATTGGCTCGTTGGAGCATTGTATCCTTATTATAAGAAATTGTTTTGA
Protein sequence

>Msa0070470

MTRSLSLSTITFHKLQSFASTNVCRRQLPHRNAVVVVKDYEMRKKRKQCIINSLHSKHCWRSGGGQCFSFLGMNLTSASFGSAVMSSVPSMTFILALIFRLERMKIKEIGSQVKAIGPIVSLEGALLMILYKGPFLQAAKSSAHTMYPENVNDPSGSHWLVGALYPYYKKLF*