AlfalfaGEDB | Msa0083250

M. sativa cultivar zhongmu-4 / Msa0083250

KEGG: K00799
GO: -
PFAM: PF02798.23

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0083250	sp\|Q03662\|GSTX1_TOBAC	45.755	212	110	3	1	211	1	208	5.88e-55	178

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0083250	Q1RSH9	93.333	225	15	0	1	225	1	225	3.44e-155	438

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0037730	Msa0083250	0.812562	3.982388e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0083250	MtrunA17_Chr1g0196711	93.333	225	15	0	1	225	1	225	6.62e-159	438
Msa0083250	MtrunA17_Chr1g0196661	81.778	225	41	0	1	225	1	225	1.10e-139	390
Msa0083250	MtrunA17_Chr1g0196721	82.791	215	37	0	4	218	3	217	5.21e-133	373
Msa0083250	MtrunA17_Chr1g0196691	78.281	221	47	1	1	220	26	246	9.67e-127	358
Msa0083250	MtrunA17_Chr4g0029341	66.977	215	71	0	3	217	4	218	7.24e-105	301
Msa0083250	MtrunA17_Chr1g0212301	47.143	210	108	2	1	208	1	209	4.66e-63	196
Msa0083250	MtrunA17_Chr3g0132581	44.595	222	111	5	1	214	1	218	4.47e-57	180
Msa0083250	MtrunA17_Chr1g0196771	44.076	211	115	2	1	210	1	209	5.52e-57	180
Msa0083250	MtrunA17_Chr4g0008791	41.905	210	118	2	2	210	6	212	9.98e-53	169
Msa0083250	MtrunA17_Chr4g0008781	43.810	210	112	3	3	210	8	213	1.08e-51	166

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0083250	AT2G29470.1	38.914	221	129	5	4	221	8	225	1.25e-50	164
Msa0083250	AT3G09270.1	42.222	225	114	7	4	220	7	223	2.64e-48	158
Msa0083250	AT2G29460.1	36.530	219	136	2	3	220	7	223	2.74e-47	155
Msa0083250	AT2G29490.1	38.813	219	129	4	4	220	8	223	2.95e-46	153
Msa0083250	AT2G29450.1	37.727	220	133	3	3	220	6	223	3.19e-46	153
Msa0083250	AT1G10370.1	39.732	224	130	3	2	220	4	227	3.63e-46	153
Msa0083250	AT1G59670.1	38.496	226	133	4	3	222	6	231	4.68e-46	153
Msa0083250	AT2G29420.1	37.963	216	132	1	3	218	9	222	1.05e-44	149
Msa0083250	AT1G59700.1	39.252	214	124	4	3	210	6	219	2.10e-43	146
Msa0083250	AT2G29480.1	36.986	219	133	4	4	220	8	223	6.43e-43	144

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Find 51 sgRNAs with CRISPR-Local

Find 164 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGTTGTGAAGAGCGAGAGA+AGG	0.595648	1_2:-62307792	Msa0083250:CDS
AATATATTTAACCCAAAATC+GGG	0.438660	1_2:+62308593	None:intergenic
AATGATCATGGACTCACATA+TGG	0.394155	1_2:+62308686	None:intergenic
AATGTATTCAACAATGATCA+TGG	0.484642	1_2:+62308674	None:intergenic
ACTCCAGTGCTAGTTCATGA+TGG	0.573621	1_2:-62308714	Msa0083250:CDS
AGAAGGCTAAAGAGAATTTA+TGG	0.203200	1_2:-62307775	Msa0083250:CDS
AGAATATCTTGCAGAGTGAA+AGG	0.542132	1_2:-62307616	Msa0083250:CDS
AGCATTGTAGACATAACTCT+TGG	0.468767	1_2:-62307683	Msa0083250:CDS
AGGTGAAACTACATGGATTT+TGG	0.314704	1_2:-62308863	Msa0083250:CDS
AGTTGTTGAGGATCGGTGTT+TGG	0.448281	1_2:-62307741	Msa0083250:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CATACAACCCTTAAAGTAAA+AGG	+	chr1_2:62307517-62307536	None:intergenic	30.0%
TATACCATATGAGAACATAG+AGG	-	chr1_2:62307555-62307574	Msa0083250:CDS	30.0%
GGTTATATTGAAGAAGTTGA+GGG	+	chr1_2:62307600-62307619	None:intergenic	30.0%
TTATAGGCAAAAACTACACT+AGG	-	chr1_2:62307871-62307890	Msa0083250:intron	30.0%
CTAAAATCGTTTGTAGTCTT+GGG	+	chr1_2:62308119-62308138	None:intergenic	30.0%
GGATCAATACTTCAAGATTT+CGG	-	chr1_2:62308508-62308527	Msa0083250:intron	30.0%
AGAAGGCTAAAGAGAATTTA+TGG	-	chr1_2:62308573-62308592	Msa0083250:intron	30.0%
GAAGGCTAAAGAGAATTTAT+GGG	-	chr1_2:62308574-62308593	Msa0083250:intron	30.0%
TGATCAGAAGAAATTCTATG+CGG	-	chr1_2:62308631-62308650	Msa0083250:CDS	30.0%
TTAAACCATAAATGCAATCG+GGG	+	chr1_2:62308761-62308780	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_2	gene	62307478	62308892	62307478	ID=Msa0083250;Name=Msa0083250
chr1_2	mRNA	62307478	62308892	62307478	ID=Msa0083250-mRNA-1;Parent=Msa0083250;Name=Msa0083250-mRNA-1;_AED=0.37;_eAED=0.37;_QI=0\|0\|0\|1\|1\|1\|2\|0\|225
chr1_2	exon	62308584	62308892	62308584	ID=Msa0083250-mRNA-1:exon:20772;Parent=Msa0083250-mRNA-1
chr1_2	exon	62307478	62307846	62307478	ID=Msa0083250-mRNA-1:exon:20771;Parent=Msa0083250-mRNA-1
chr1_2	CDS	62308584	62308892	62308584	ID=Msa0083250-mRNA-1:cds;Parent=Msa0083250-mRNA-1
chr1_2	CDS	62307478	62307846	62307478	ID=Msa0083250-mRNA-1:cds;Parent=Msa0083250-mRNA-1

Gene Sequence

>Msa0083250

ATGGCAGAGGTGAAACTACATGGATTTTGGTACAGTCCTTTTACTTTAAGGGTTGTATGGACCCTAAATTTGAAAGGTATACCATATGAGAACATAGAGGAAGATCGTTTCAATAAGAGCCCTCAACTTCTTCAATATAACCCAGTACGCAAAAATACTCCAGTGCTAGTTCATGATGGAAAACCCATATGTGAGTCCATGATCATTGTTGAATACATTGATGAGATATGGCCACACAACTCATTGCTTCCTGCTCATCCCTATGATAAAGCTCAAGCCCGATTTTGGGTTAAATATATTGATGGCATGATTTCGGCGATTGAGGCATTCCGCCATAGCATAAGTTGTGAAGAGCGAGAGAAGGCTAAAGAGAATTTATGGGCGAGACTCAAAGTTGTTGAGGATCGGTGTTTGGATGATCAGAAGAAATTCTATGCGGGAGACAACGTTAGCATTGTAGACATAACTCTTGGATCATTTGTCAAATTTATTGAAGTTCAAGAAGATATATTTGAAGAGAATATCTTGCAGAGTGAAAGGTTCCCCCGATTGCATTTATGGTTTAATAATTTCAAGGATGTTCCAGTCATCAAAGAAAACACCCCTGACCAAGAGAAGTTGGTGGCTTTTCATAATCTTCTAATTGATAAAATCAAGGCAATCAAGACATCTTCTTGA

Protein sequence

>Msa0083250

MAEVKLHGFWYSPFTLRVVWTLNLKGIPYENIEEDRFNKSPQLLQYNPVRKNTPVLVHDGKPICESMIIVEYIDEIWPHNSLLPAHPYDKAQARFWVKYIDGMISAIEAFRHSISCEEREKAKENLWARLKVVEDRCLDDQKKFYAGDNVSIVDITLGSFVKFIEVQEDIFEENILQSERFPRLHLWFNNFKDVPVIKENTPDQEKLVAFHNLLIDKIKAIKTSS*