AlfalfaGEDB | Msa0084470

M. sativa cultivar zhongmu-4 / Msa0084470

KEGG: K07560|K11644
GO: GO:0000003 GO:0001708 GO:0002119 GO:0002164 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0007275 GO:0007423 GO:0007548 GO:0008150 GO:0009653 GO:0009791 GO:0009887 GO:0009987 GO:0022414 GO:0030154 GO:0030539 GO:0032501 GO:0032502 GO:0035112 GO:0040011 GO:0040025 GO:0045165 GO:0046661 GO:0048513 GO:0048569 GO:0048608 GO:0048731 GO:0048806 GO:0048808 GO:0048856 GO:0048869 GO:0061458 GO:0090596 GO:0090597 GO:0090598
PFAM: PF02671.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0084470	XP_013459511.1	68.732	355	104	4	77	429	1	350	1.73e-159	464

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0084470	sp\|Q9SRH9\|SNL1_ARATH	34.161	161	87	5	8	164	56	201	1.58e-15	82.8

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0084470	A0A072UV92	68.732	355	104	4	77	429	1	350	8.28e-160	464

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TFs/TRs:

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Gene ID	Type	Classification
Msa0084470	TR	Others

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0084470	Msa0176230	0.809903	1.503504e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0084470	MtrunA17_Chr3g0093911	68.732	355	104	4	77	429	1	350	1.59e-163	464
Msa0084470	MtrunA17_Chr7g0252101	60.706	425	152	10	12	429	16	432	8.74e-161	460
Msa0084470	MtrunA17_Chr1g0207351	64.045	356	116	6	81	429	16	366	1.26e-143	414
Msa0084470	MtrunA17_Chr4g0075971	54.110	438	175	8	10	429	173	602	3.77e-133	395
Msa0084470	MtrunA17_Chr6g0488101	48.359	457	187	15	11	429	10	455	9.40e-123	364
Msa0084470	MtrunA17_Chr3g0077801	55.056	356	153	5	77	429	1	352	1.74e-119	352
Msa0084470	MtrunA17_Chr6g0488271	49.522	418	170	13	43	429	8	415	5.83e-117	348
Msa0084470	MtrunA17_Chr3g0077901	50.552	362	166	5	73	428	17	371	6.15e-111	331
Msa0084470	MtrunA17_Chr3g0077851	52.802	339	147	5	96	428	4	335	1.58e-109	326
Msa0084470	MtrunA17_Chr1g0209051	62.545	275	94	4	158	428	1	270	3.39e-107	317

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0084470	AT3G01320.1	34.161	161	87	5	8	164	56	201	1.61e-16	82.8
Msa0084470	AT3G01320.2	34.161	161	87	5	8	164	56	201	1.62e-16	82.8
Msa0084470	AT1G24190.3	41.414	99	51	2	8	106	13	104	1.03e-15	80.1
Msa0084470	AT1G24190.2	41.414	99	51	2	8	106	13	104	1.08e-15	80.1
Msa0084470	AT1G24190.1	41.414	99	51	2	8	106	13	104	1.09e-15	80.1
Msa0084470	AT5G15020.1	37.815	119	63	3	8	125	51	159	1.40e-15	79.7
Msa0084470	AT5G15020.2	37.815	119	63	3	8	125	51	159	1.70e-15	79.7
Msa0084470	AT1G59890.4	46.988	83	41	2	9	89	45	126	1.23e-14	77.0
Msa0084470	AT1G59890.1	46.988	83	41	2	9	89	45	126	1.39e-14	76.6
Msa0084470	AT1G59890.3	46.988	83	41	2	9	89	45	126	1.41e-14	76.6

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Find 93 sgRNAs with CRISPR-Local

Find 167 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTCCTGAAAGATTTGA+GGG	0.424484	1_2:-63947650	Msa0084470:CDS
AACAATAGAAAATAGAGAGT+GGG	0.566020	1_2:-63945564	Msa0084470:CDS
AACTATGCAAATCAGTTCAA+TGG	0.478283	1_2:-63947877	Msa0084470:five_prime_UTR
AAGAGTTACCTTCAGAGGTA+GGG	0.509565	1_2:+63945711	None:intergenic
AAGATGAAGAATTTCCGAAT+CGG	0.560007	1_2:-63947748	Msa0084470:CDS
AAGGAACAAGAAAACACGAC+AGG	0.566522	1_2:+63947979	None:intergenic
AATATATGTCATCACTAACG+AGG	0.731941	1_2:-63945513	Msa0084470:CDS
AATATGTAACATAGCTAGAG+CGG	0.634773	1_2:+63945816	None:intergenic
AATATGTAGCGTTTATTCAG+GGG	0.598392	1_2:-63945168	Msa0084470:CDS
AATCGAGTGAAGGAGGATTG+AGG	0.594289	1_2:+63947848	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACTATGCAAATCAGTTCAA+TGG	-	chr1_2:63944981-63945000	Msa0084470:three_prime_UTR	30.0%
AAGATGAAGAATTTCCGAAT+CGG	-	chr1_2:63945110-63945129	Msa0084470:CDS	30.0%
TAAAACTCCTGAAAGATTTG+AGG	-	chr1_2:63945207-63945226	Msa0084470:CDS	30.0%
AAAACTCCTGAAAGATTTGA+GGG	-	chr1_2:63945208-63945227	Msa0084470:CDS	30.0%
GGAAGAAGAATTGATATAAG+AGG	-	chr1_2:63945233-63945252	Msa0084470:CDS	30.0%
AAAAGTAGTAACTAACCAGT+TGG	+	chr1_2:63945329-63945348	None:intergenic	30.0%
ATCTCACTTTCAATTACGAT+TGG	-	chr1_2:63945432-63945451	Msa0084470:CDS	30.0%
TAACATGATGAACAACTACA+TGG	+	chr1_2:63945754-63945773	None:intergenic	30.0%
ATATACGTATTGTATTGCTG+TGG	-	chr1_2:63945778-63945797	Msa0084470:CDS	30.0%
CTTATTCTACTAAGTGTGAT+CGG	+	chr1_2:63946048-63946067	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_2	gene	63944840	63948040	63944840	ID=Msa0084470;Name=Msa0084470
chr1_2	mRNA	63944840	63948040	63944840	ID=Msa0084470-mRNA-1;Parent=Msa0084470;Name=Msa0084470-mRNA-1;_AED=0.01;_eAED=0.01;_QI=273\|1\|1\|1\|1\|1\|2\|197\|429
chr1_2	exon	63944840	63946106	63944840	ID=Msa0084470-mRNA-1:exon:21470;Parent=Msa0084470-mRNA-1
chr1_2	exon	63947548	63948040	63947548	ID=Msa0084470-mRNA-1:exon:21469;Parent=Msa0084470-mRNA-1
chr1_2	five_prime_UTR	63947768	63948040	63947768	ID=Msa0084470-mRNA-1:five_prime_utr;Parent=Msa0084470-mRNA-1
chr1_2	CDS	63947548	63947767	63947548	ID=Msa0084470-mRNA-1:cds;Parent=Msa0084470-mRNA-1
chr1_2	CDS	63945037	63946106	63945037	ID=Msa0084470-mRNA-1:cds;Parent=Msa0084470-mRNA-1
chr1_2	three_prime_UTR	63944840	63945036	63944840	ID=Msa0084470-mRNA-1:three_prime_utr;Parent=Msa0084470-mRNA-1

Gene Sequence

>Msa0084470

ATGAAGAATTTCCGAATCGGCGACGATAAACTAGGGTTTCTCAAGGCAGTGAAAGCTGCATTTGAATACAACAGAATAAAGTATCATCAGTTTTTAAAACTCCTGAAAGATTTGAGGGCTGGAAGAAGAATTGATATAAGAGGTGTCATGACTAGAGTGAAGAAGCTGTTTAAAGGACATACAGATTTAATTATGGGATTCAAAATTTTCCTTCCAACTGGGAAAAAGGTGAAGAAGGTAGAAGAGAACTATGAACTTCCTTTGGATATGTTTGATATCACTTGCAAAACATTAGATTTTGACGACCTATTTCAGTTTTCCGGCGTGTGCAAGAATTGGAGGACTTTTCATAAATTTTACTGGAGAATTTTTTTGGCATCCCAAGAACCATTACTTCTTCATATATCGCCTTATGCTAAGAGTTATTATTCCTTTATTAGCATACCCAACCAAAAAGTTTATCACTTGGAGATGATGGATTACTTCATCCGCTCTAGCTATGTTACATATTCTAGCGGATATTTTATACTCGCAAAGTGCAATAATTCATTTCTACTAATCAATCCATTTACAAGAATAATGAAATCAATTCCCCCTACCTCTGAAGGTAACTCTTCTTTAACTATGTGTGCCTTGCTTGCTTTTAGCAAATGCTCTGAGGAATTTGTCTTTGTGGTTTTATGCAAACAACTACGTTTGCATGTCTATCAATCTCGAAATTGTGGATGGGTTACTTATTCAACAATAGAAAATAGAGAGTGGGTTGTTGACTTTGTGGTTTTGCATGATATAATATATGTCATCACTAACGAGGCGAAGATAGGGGTATTGAGCTTGAATTCTGCAAATATTAAATTTCTTAATCTGAGGAGTACTCCCGTCATAGGAATTCCTAGTTCCCTTAGGTTGGTTATTTGTGATGAGCAACTTTTAGTAGTTTATTTCTTGGCCAATGAAAGTGCAGATCTGTACAAGATAGACTTCTCAACCATGAATTATGTCAGACTGAAAACTTTGGGCGACATTGCATTATTTTCTATTTCGTTTGGTATCAAGAAAAAATGTTATGCATTGAGCAACCCGAACAAGTGGGGATATGAGAGCAACTCTGTGTATTACATTGGTGCTTCTTCAACAATATGTAGCGTTTATTCAGGGGATGATAAAAAATTGCAAAAATGCATTACGCTCCCATCTCTTCTTGAATCTCATCCTACAATTGTTGGTTGGAACGCCGATTGGTGTTTTAGGCATCTAAAATATGAGGTAGATTATTCTCTAGTTGAGTAA

Protein sequence

>Msa0084470

MKNFRIGDDKLGFLKAVKAAFEYNRIKYHQFLKLLKDLRAGRRIDIRGVMTRVKKLFKGHTDLIMGFKIFLPTGKKVKKVEENYELPLDMFDITCKTLDFDDLFQFSGVCKNWRTFHKFYWRIFLASQEPLLLHISPYAKSYYSFISIPNQKVYHLEMMDYFIRSSYVTYSSGYFILAKCNNSFLLINPFTRIMKSIPPTSEGNSSLTMCALLAFSKCSEEFVFVVLCKQLRLHVYQSRNCGWVTYSTIENREWVVDFVVLHDIIYVITNEAKIGVLSLNSANIKFLNLRSTPVIGIPSSLRLVICDEQLLVVYFLANESADLYKIDFSTMNYVRLKTLGDIALFSISFGIKKKCYALSNPNKWGYESNSVYYIGASSTICSVYSGDDKKLQKCITLPSLLESHPTIVGWNADWCFRHLKYEVDYSLVE*