Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0176230 | XP_013459511.1 | 69.014 | 355 | 103 | 4 | 77 | 429 | 1 | 350 | 6.48e-160 | 465 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0176230 | sp|Q9SRH9|SNL1_ARATH | 33.537 | 164 | 90 | 5 | 5 | 164 | 53 | 201 | 1.42e-15 | 82.8 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0176230 | A0A072UV92 | 69.014 | 355 | 103 | 4 | 77 | 429 | 1 | 350 | 3.10e-160 | 465 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0176230 | TR | Others |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0084470 | Msa0176230 | 0.809903 | 1.503504e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0176230 | MtrunA17_Chr3g0093911 | 69.014 | 355 | 103 | 4 | 77 | 429 | 1 | 350 | 5.96e-164 | 465 |
| Msa0176230 | MtrunA17_Chr7g0252101 | 60.185 | 432 | 155 | 11 | 7 | 429 | 9 | 432 | 2.97e-161 | 461 |
| Msa0176230 | MtrunA17_Chr1g0207351 | 64.326 | 356 | 115 | 6 | 81 | 429 | 16 | 366 | 3.26e-144 | 415 |
| Msa0176230 | MtrunA17_Chr4g0075971 | 54.054 | 444 | 175 | 9 | 7 | 429 | 167 | 602 | 1.29e-133 | 397 |
| Msa0176230 | MtrunA17_Chr6g0488101 | 48.261 | 460 | 189 | 15 | 8 | 429 | 7 | 455 | 1.38e-123 | 366 |
| Msa0176230 | MtrunA17_Chr3g0077801 | 55.337 | 356 | 152 | 5 | 77 | 429 | 1 | 352 | 5.67e-120 | 353 |
| Msa0176230 | MtrunA17_Chr6g0488271 | 49.522 | 418 | 170 | 13 | 43 | 429 | 8 | 415 | 5.12e-117 | 348 |
| Msa0176230 | MtrunA17_Chr3g0077901 | 50.829 | 362 | 165 | 5 | 73 | 428 | 17 | 371 | 2.91e-111 | 332 |
| Msa0176230 | MtrunA17_Chr3g0077851 | 53.097 | 339 | 146 | 5 | 96 | 428 | 4 | 335 | 5.69e-110 | 327 |
| Msa0176230 | MtrunA17_Chr1g0209051 | 62.545 | 275 | 94 | 4 | 158 | 428 | 1 | 270 | 2.78e-107 | 318 |
| Msa0176230 | MtrunA17_Chr3g0112311 | 51.657 | 362 | 157 | 9 | 77 | 427 | 1 | 355 | 4.10e-105 | 315 |
| Msa0176230 | MtrunA17_Chr7g0243331 | 52.137 | 351 | 159 | 7 | 82 | 429 | 6 | 350 | 4.52e-102 | 307 |
| Msa0176230 | MtrunA17_Chr6g0452641 | 49.363 | 314 | 142 | 7 | 127 | 429 | 33 | 340 | 9.43e-79 | 247 |
| Msa0176230 | MtrunA17_Chr3g0122991 | 44.308 | 325 | 117 | 7 | 79 | 391 | 2 | 274 | 3.79e-73 | 230 |
| Msa0176230 | MtrunA17_Chr1g0202841 | 63.536 | 181 | 64 | 1 | 249 | 429 | 1 | 179 | 1.64e-71 | 223 |
| Msa0176230 | MtrunA17_Chr3g0123471 | 49.627 | 268 | 122 | 8 | 158 | 421 | 1 | 259 | 1.21e-64 | 208 |
| Msa0176230 | MtrunA17_Chr4g0075861 | 53.202 | 203 | 78 | 3 | 10 | 197 | 173 | 373 | 2.83e-52 | 179 |
| Msa0176230 | MtrunA17_Chr7g0243391 | 45.794 | 214 | 111 | 3 | 73 | 283 | 14 | 225 | 7.36e-51 | 172 |
| Msa0176230 | MtrunA17_Chr1g0206951 | 62.393 | 117 | 43 | 1 | 80 | 195 | 2 | 118 | 2.15e-41 | 143 |
| Msa0176230 | MtrunA17_Chr1g0196231 | 56.410 | 156 | 61 | 4 | 65 | 217 | 215 | 366 | 3.13e-40 | 147 |
| Msa0176230 | MtrunA17_Chr1g0187941 | 56.934 | 137 | 59 | 0 | 249 | 385 | 1 | 137 | 3.89e-40 | 140 |
| Msa0176230 | MtrunA17_Chr6g0486591 | 48.466 | 163 | 71 | 5 | 276 | 429 | 1 | 159 | 4.33e-38 | 135 |
| Msa0176230 | MtrunA17_Chr1g0209191 | 60.396 | 101 | 34 | 2 | 331 | 428 | 1 | 98 | 5.46e-32 | 117 |
| Msa0176230 | MtrunA17_Chr1g0196241 | 70.886 | 79 | 19 | 2 | 322 | 399 | 3 | 78 | 4.17e-29 | 109 |
| Msa0176230 | MtrunA17_Chr1g0209061 | 53.211 | 109 | 39 | 3 | 9 | 116 | 10 | 107 | 4.33e-28 | 107 |
| Msa0176230 | MtrunA17_Chr6g0459061 | 29.143 | 350 | 228 | 11 | 85 | 426 | 18 | 355 | 1.04e-26 | 110 |
| Msa0176230 | MtrunA17_Chr8g0364231 | 39.669 | 121 | 66 | 3 | 5 | 125 | 38 | 151 | 5.19e-18 | 87.0 |
| Msa0176230 | MtrunA17_Chr7g0238781 | 42.222 | 135 | 71 | 5 | 109 | 239 | 27 | 158 | 7.21e-18 | 80.5 |
| Msa0176230 | MtrunA17_Chr3g0077911 | 64.615 | 65 | 21 | 2 | 9 | 72 | 90 | 153 | 5.77e-16 | 75.1 |
| Msa0176230 | MtrunA17_Chr1g0205861 | 44.048 | 84 | 46 | 1 | 2 | 85 | 9 | 91 | 1.40e-15 | 72.8 |
| Msa0176230 | MtrunA17_Chr5g0415031 | 40.187 | 107 | 57 | 3 | 5 | 111 | 39 | 138 | 1.52e-15 | 79.3 |
| Msa0176230 | MtrunA17_Chr1g0209201 | 55.224 | 67 | 28 | 2 | 12 | 78 | 19 | 83 | 3.60e-15 | 72.4 |
| Msa0176230 | MtrunA17_Chr3g0077861 | 63.077 | 65 | 22 | 2 | 9 | 72 | 90 | 153 | 5.67e-15 | 72.4 |
| Msa0176230 | MtrunA17_Chr1g0206781 | 45.000 | 80 | 38 | 2 | 11 | 85 | 13 | 91 | 1.13e-14 | 70.1 |
| Msa0176230 | MtrunA17_Chr6g0452001 | 35.537 | 121 | 71 | 2 | 5 | 125 | 59 | 172 | 2.41e-14 | 75.5 |
| Msa0176230 | MtrunA17_Chr6g0452021 | 35.897 | 117 | 66 | 3 | 9 | 125 | 43 | 150 | 1.20e-13 | 72.4 |
| Msa0176230 | MtrunA17_Chr1g0205811 | 38.679 | 106 | 52 | 3 | 9 | 109 | 14 | 111 | 8.20e-13 | 65.1 |
| Msa0176230 | MtrunA17_Chr7g0218911 | 48.387 | 62 | 31 | 1 | 13 | 74 | 2 | 62 | 1.07e-12 | 64.7 |
| Msa0176230 | MtrunA17_Chr1g0206771 | 48.438 | 64 | 32 | 1 | 11 | 74 | 18 | 80 | 1.75e-12 | 65.9 |
| Msa0176230 | MtrunA17_Chr1g0206771 | 46.377 | 69 | 36 | 1 | 4 | 72 | 90 | 157 | 6.69e-12 | 63.9 |
| Msa0176230 | MtrunA17_Chr5g0431721 | 48.684 | 76 | 35 | 3 | 2 | 74 | 5 | 79 | 4.26e-12 | 62.8 |
| Msa0176230 | MtrunA17_Chr8g0364021 | 37.255 | 102 | 60 | 2 | 5 | 103 | 40 | 140 | 3.12e-11 | 63.2 |
| Msa0176230 | MtrunA17_Chr3g0077811 | 44.304 | 79 | 41 | 3 | 1 | 78 | 6 | 82 | 4.62e-11 | 60.1 |
| Msa0176230 | MtrunA17_Chr3g0077891 | 50.769 | 65 | 30 | 2 | 9 | 72 | 99 | 162 | 6.18e-11 | 61.2 |
| Msa0176230 | MtrunA17_Chr3g0077881 | 45.161 | 93 | 41 | 4 | 1 | 92 | 1 | 84 | 6.48e-11 | 61.2 |
| Msa0176230 | MtrunA17_Chr3g0077941 | 47.887 | 71 | 35 | 2 | 3 | 72 | 92 | 161 | 9.41e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0176230 | AT3G01320.2 | 33.537 | 164 | 90 | 5 | 5 | 164 | 53 | 201 | 1.42e-16 | 82.8 |
| Msa0176230 | AT3G01320.1 | 33.537 | 164 | 90 | 5 | 5 | 164 | 53 | 201 | 1.45e-16 | 82.8 |
| Msa0176230 | AT1G24190.3 | 41.176 | 102 | 53 | 2 | 5 | 106 | 10 | 104 | 4.30e-16 | 81.6 |
| Msa0176230 | AT1G24190.1 | 46.429 | 84 | 44 | 1 | 5 | 88 | 10 | 92 | 4.65e-16 | 81.3 |
| Msa0176230 | AT1G24190.2 | 41.176 | 102 | 53 | 2 | 5 | 106 | 10 | 104 | 4.73e-16 | 81.3 |
| Msa0176230 | AT5G15020.1 | 36.066 | 122 | 67 | 3 | 5 | 125 | 48 | 159 | 7.16e-15 | 77.8 |
| Msa0176230 | AT5G15020.2 | 36.066 | 122 | 67 | 3 | 5 | 125 | 48 | 159 | 7.34e-15 | 77.8 |
| Msa0176230 | AT1G59890.4 | 48.193 | 83 | 40 | 2 | 9 | 89 | 45 | 126 | 7.40e-15 | 77.4 |
| Msa0176230 | AT1G59890.1 | 48.193 | 83 | 40 | 2 | 9 | 89 | 45 | 126 | 8.25e-15 | 77.4 |
| Msa0176230 | AT1G59890.2 | 48.193 | 83 | 40 | 2 | 9 | 89 | 45 | 126 | 8.48e-15 | 77.4 |
| Msa0176230 | AT1G59890.3 | 48.193 | 83 | 40 | 2 | 9 | 89 | 45 | 126 | 8.69e-15 | 77.4 |
| Msa0176230 | AT1G70060.2 | 51.429 | 70 | 33 | 1 | 5 | 74 | 10 | 78 | 1.20e-14 | 77.0 |
| Msa0176230 | AT1G70060.1 | 51.429 | 70 | 33 | 1 | 5 | 74 | 10 | 78 | 1.20e-14 | 77.0 |
| Msa0176230 | AT1G10450.2 | 52.174 | 69 | 32 | 1 | 9 | 77 | 33 | 100 | 5.99e-13 | 71.6 |
| Msa0176230 | AT1G10450.1 | 52.174 | 69 | 32 | 1 | 9 | 77 | 33 | 100 | 5.99e-13 | 71.6 |
| Msa0176230 | AT1G10450.3 | 52.174 | 69 | 32 | 1 | 9 | 77 | 84 | 151 | 6.86e-13 | 71.2 |
| Msa0176230 | AT5G15025.1 | 40.244 | 82 | 48 | 1 | 2 | 83 | 40 | 120 | 1.91e-12 | 64.3 |
| Msa0176230 | AT1G70030.3 | 37.615 | 109 | 56 | 3 | 5 | 111 | 18 | 116 | 5.28e-12 | 63.2 |
| Msa0176230 | AT1G70030.2 | 38.776 | 98 | 52 | 2 | 9 | 106 | 22 | 111 | 6.02e-12 | 63.5 |
| Msa0176230 | AT1G24200.1 | 47.761 | 67 | 34 | 1 | 8 | 74 | 13 | 78 | 1.59e-11 | 63.5 |
| Msa0176230 | AT1G70030.1 | 38.095 | 105 | 53 | 3 | 9 | 111 | 11 | 105 | 1.81e-11 | 62.8 |
Find 93 sgRNAs with CRISPR-Local
Find 155 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTTCACTCGATTTATTTCT+TGG | 0.092423 | 1_4:+62487297 | Msa0176230:five_prime_UTR |
| TTCCAGCCCTCAAATCTTTC+AGG | 0.146764 | 1_4:-62487497 | None:intergenic |
| TTATTTGTAGTTTCTGAATT+AGG | 0.165086 | 1_4:+62487187 | Msa0176230:five_prime_UTR |
| TTCTACATTAGTCAAATTAT+TGG | 0.171653 | 1_4:-62489961 | None:intergenic |
| GATTTCATTATTCTTGTAAA+TGG | 0.220120 | 1_4:-62489172 | None:intergenic |
| ACAATATGTAGCGTTTATTC+AGG | 0.224490 | 1_4:+62489742 | Msa0176230:CDS |
| CTCTCATATCCCCACTTGTT+CGG | 0.274296 | 1_4:-62489688 | None:intergenic |
| TATTTGTAGTTTCTGAATTA+GGG | 0.283688 | 1_4:+62487188 | Msa0176230:five_prime_UTR |
| TTCAGAAACTACAAATAAAA+AGG | 0.297870 | 1_4:-62487182 | None:intergenic |
| CAAATTATTGGCAAACAAAA+AGG | 0.304170 | 1_4:-62489949 | None:intergenic |
| CTCTGAGGAATTTGTCTTTG+TGG | 0.313965 | 1_4:+62489261 | Msa0176230:CDS |
| TTTCAGTCTGACATAATTCA+TGG | 0.325552 | 1_4:-62489596 | None:intergenic |
| TGTTGTGATAAAAGGGAAAA+AGG | 0.329108 | 1_4:+62488814 | Msa0176230:intron |
| AGAGTGAAGAAGCTGTTTAA+AGG | 0.330293 | 1_4:+62487549 | Msa0176230:CDS |
| TATGAAGAAGTAATGGTTCT+TGG | 0.336847 | 1_4:-62488988 | None:intergenic |
| GAATCTCATCCTACAATTGT+TGG | 0.346564 | 1_4:+62489811 | Msa0176230:CDS |
| AATCGAGTGAAGGAGGATTA+AGG | 0.348459 | 1_4:-62487287 | None:intergenic |
| TCTCATATCCCCACTTGTTC+GGG | 0.349130 | 1_4:-62489687 | None:intergenic |
| AGGACATACAGATTTAGTTC+TGG | 0.360154 | 1_4:+62487569 | Msa0176230:CDS |
| GTACACATTTGCACTTTCAT+TGG | 0.360934 | 1_4:-62489557 | None:intergenic |
| AGTTTATCACTTGGAGATGA+TGG | 0.375141 | 1_4:+62489063 | Msa0176230:CDS |
| TTTCCGGCGTGTGCAAGAAT+TGG | 0.380354 | 1_4:+62488924 | Msa0176230:CDS |
| GGACATACAGATTTAGTTCT+GGG | 0.388137 | 1_4:+62487570 | Msa0176230:CDS |
| TTGGTTGGGTATGCTAATAA+AGG | 0.390018 | 1_4:-62489038 | None:intergenic |
| GGATGAGATTCAAGAAGAGA+TGG | 0.394418 | 1_4:-62489799 | None:intergenic |
| AGAGAACTATGAACTTCCTT+TGG | 0.396409 | 1_4:+62488850 | Msa0176230:CDS |
| CTAGTTTATCGTGGCCGATT+AGG | 0.401292 | 1_4:-62487407 | None:intergenic |
| ACACGCCGGAAAACTGAAAT+AGG | 0.402270 | 1_4:-62488913 | None:intergenic |
| ATCACTAACGAGGCGAAGAT+AGG | 0.404482 | 1_4:+62489409 | Msa0176230:CDS |
| CTAATCGGCCACGATAAACT+AGG | 0.408504 | 1_4:+62487408 | Msa0176230:CDS |
| ATTGTACATTGTGTTACTCT+TGG | 0.412290 | 1_4:+62489905 | Msa0176230:three_prime_UTR |
| TAAAACTCCTGAAAGATTTG+AGG | 0.415830 | 1_4:+62487490 | Msa0176230:CDS |
| AAAACTCCTGAAAGATTTGA+GGG | 0.424484 | 1_4:+62487491 | Msa0176230:CDS |
| CTGAGGAGTACTCCCGTCAT+AGG | 0.433963 | 1_4:+62489472 | Msa0176230:CDS |
| TATGTCAGACTGAAAACTTT+GGG | 0.448246 | 1_4:+62489604 | Msa0176230:CDS |
| CCGGCGTGTGCAAGAATTGG+AGG | 0.450628 | 1_4:+62488927 | Msa0176230:CDS |
| TAGGAATTCCTAGTTCCCTT+AGG | 0.453013 | 1_4:+62489491 | Msa0176230:CDS |
| ATGAAGAAGTAATGGTTCTT+GGG | 0.453465 | 1_4:-62488987 | None:intergenic |
| GCGTTCCAACCAACAATTGT+AGG | 0.458516 | 1_4:-62489820 | None:intergenic |
| CTCCTGAAAGATTTGAGGGC+TGG | 0.459564 | 1_4:+62487495 | Msa0176230:CDS |
| TTATGTCAGACTGAAAACTT+TGG | 0.462565 | 1_4:+62489603 | Msa0176230:CDS |
| AACTATGCAAATCAGTTCAA+TGG | 0.478283 | 1_4:+62487258 | Msa0176230:five_prime_UTR |
| GAATAATAACTCTTAGCATA+AGG | 0.482018 | 1_4:-62489016 | None:intergenic |
| AAGATGAAGAACTTCCTAAT+CGG | 0.484584 | 1_4:+62487393 | Msa0176230:exon |
| CGTATCAAAATTAAATGTGT+TGG | 0.485240 | 1_4:+62490072 | Msa0176230:three_prime_UTR |
| GTCTATCAATCTCGAAATTG+TGG | 0.493729 | 1_4:+62489310 | Msa0176230:CDS |
| AGAGTGGGTTGTTGACTTTG+TGG | 0.494217 | 1_4:+62489363 | Msa0176230:CDS |
| ATAACCAACCTAAGGGAACT+AGG | 0.496475 | 1_4:-62489499 | None:intergenic |
| CAATATGTAGCGTTTATTCA+GGG | 0.497622 | 1_4:+62489743 | Msa0176230:CDS |
| CATCACAAATAACCAACCTA+AGG | 0.498767 | 1_4:-62489507 | None:intergenic |
| TCACTAACGAGGCGAAGATA+GGG | 0.506057 | 1_4:+62489410 | Msa0176230:CDS |
| AATTCCTAGTTCCCTTAGGT+TGG | 0.508434 | 1_4:+62489495 | Msa0176230:CDS |
| GGAAGAAGAATTGATATAAG+AGG | 0.508673 | 1_4:+62487516 | Msa0176230:CDS |
| GAAGAGTTACCTTCAGAGGT+AGG | 0.509259 | 1_4:-62489202 | None:intergenic |
| AAGAGTTACCTTCAGAGGTA+GGG | 0.509565 | 1_4:-62489201 | None:intergenic |
| TAGATGCCTAAAACACCAAT+CGG | 0.521949 | 1_4:-62489842 | None:intergenic |
| ATCAATCTCGAAATTGTGGA+TGG | 0.526711 | 1_4:+62489314 | Msa0176230:CDS |
| GTTGAACGAGCAGCCGTGGA+AGG | 0.529951 | 1_4:-62487238 | None:intergenic |
| TAAAGAAGAGTTACCTTCAG+AGG | 0.531919 | 1_4:-62489206 | None:intergenic |
| AGAGTTACCTTCAGAGGTAG+GGG | 0.532859 | 1_4:-62489200 | None:intergenic |
| CTCATCCTACAATTGTTGGT+TGG | 0.536664 | 1_4:+62489815 | Msa0176230:CDS |
| GATGAGATTCAAGAAGAGAT+GGG | 0.537227 | 1_4:-62489798 | None:intergenic |
| CATAGTTGAACGAGCAGCCG+TGG | 0.548445 | 1_4:-62487242 | None:intergenic |
| GGCGATATATGAAGAAGTAA+TGG | 0.551671 | 1_4:-62488995 | None:intergenic |
| ATATTAAATTTCTTAATCTG+AGG | 0.557985 | 1_4:+62489455 | Msa0176230:CDS |
| AACAATAGAAAATAGAGAGT+GGG | 0.566020 | 1_4:+62489348 | Msa0176230:CDS |
| TAATCGGCCACGATAAACTA+GGG | 0.576900 | 1_4:+62487409 | Msa0176230:CDS |
| AGAAATAAATCGAGTGAAGG+AGG | 0.577568 | 1_4:-62487294 | None:intergenic |
| CAACAATAGAAAATAGAGAG+TGG | 0.582774 | 1_4:+62489347 | Msa0176230:CDS |
| GCATTTGCTAAAAGCAAGCA+AGG | 0.585085 | 1_4:-62489239 | None:intergenic |
| CATTGAGCAACCCGAACAAG+TGG | 0.585822 | 1_4:+62489677 | Msa0176230:CDS |
| TTGAACGAGCAGCCGTGGAA+GGG | 0.593904 | 1_4:-62487237 | None:intergenic |
| GAACTAGGAATTCCTATGAC+GGG | 0.594218 | 1_4:-62489484 | None:intergenic |
| CCAAGAAATAAATCGAGTGA+AGG | 0.596858 | 1_4:-62487297 | None:intergenic |
| AATATGTAGCGTTTATTCAG+GGG | 0.598392 | 1_4:+62489744 | Msa0176230:CDS |
| GTGATATCAAACATATCCAA+AGG | 0.599034 | 1_4:-62488866 | None:intergenic |
| TTGTTGGTTGGAACGCCGAT+TGG | 0.602949 | 1_4:+62489827 | Msa0176230:CDS |
| ATCACAAATAACCAACCTAA+GGG | 0.609657 | 1_4:-62489506 | None:intergenic |
| GTAGTAACTAACCAGTTGGA+AGG | 0.611566 | 1_4:-62487605 | None:intergenic |
| CACTCGAATCATCCCTTCCA+CGG | 0.613744 | 1_4:+62487225 | Msa0176230:five_prime_UTR |
| TCAATCTCGAAATTGTGGAT+GGG | 0.618953 | 1_4:+62489315 | Msa0176230:CDS |
| GGAACTAGGAATTCCTATGA+CGG | 0.624231 | 1_4:-62489485 | None:intergenic |
| CACTAACGAGGCGAAGATAG+GGG | 0.627150 | 1_4:+62489411 | Msa0176230:CDS |
| CGATAAACTAGGGTTTCTCA+AGG | 0.627978 | 1_4:+62487419 | Msa0176230:CDS |
| AATATGTAACATAGCTAGAG+CGG | 0.634773 | 1_4:-62489096 | None:intergenic |
| ATTGAGCAACCCGAACAAGT+GGG | 0.652805 | 1_4:+62489678 | Msa0176230:CDS |
| CCTCCAATTCTTGCACACGC+CGG | 0.653779 | 1_4:-62488927 | None:intergenic |
| AGCAACTCTGTGTATTACAT+TGG | 0.654518 | 1_4:+62489709 | Msa0176230:CDS |
| TGAGAAACCCTAGTTTATCG+TGG | 0.656216 | 1_4:-62487416 | None:intergenic |
| TTGAGCAACCCGAACAAGTG+GGG | 0.657706 | 1_4:+62489679 | Msa0176230:CDS |
| AGCTATGTTACATATTCTAG+CGG | 0.658771 | 1_4:+62489103 | Msa0176230:CDS |
| GAGTTACCTTCAGAGGTAGG+GGG | 0.669065 | 1_4:-62489199 | None:intergenic |
| AATATATGTCATCACTAACG+AGG | 0.731941 | 1_4:+62489399 | Msa0176230:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GAAATAAATAAAAAAACATA+TGG | + | chr1_4:62488767-62488786 | Msa0176230:intron | 10.0% |
| !!! | TTGGTTTTATTATTTTGAAT+TGG | + | chr1_4:62487316-62487335 | Msa0176230:five_prime_UTR | 15.0% |
| !!! | TTTTAACAAAATAGTTTTAG+AGG | + | chr1_4:62487714-62487733 | Msa0176230:intron | 15.0% |
| !! | ATTTATGTCTATTATTTATG+TGG | + | chr1_4:62487780-62487799 | Msa0176230:intron | 15.0% |
| !!! | ACTTGATTAATTTTTGTTTT+AGG | + | chr1_4:62488128-62488147 | Msa0176230:intron | 15.0% |
| !!! | CTTGATTAATTTTTGTTTTA+GGG | + | chr1_4:62488129-62488148 | Msa0176230:intron | 15.0% |
| !!! | TTGATTAATTTTTGTTTTAG+GGG | + | chr1_4:62488130-62488149 | Msa0176230:intron | 15.0% |
| !!! | TTTGGTTTTATATATCATAT+TGG | - | chr1_4:62488409-62488428 | None:intergenic | 15.0% |
| !!! | TTTTTGTATATGCTATAATT+TGG | - | chr1_4:62488427-62488446 | None:intergenic | 15.0% |
| !! | ATATTAAATTTCTTAATCTG+AGG | + | chr1_4:62489455-62489474 | Msa0176230:CDS | 15.0% |
| !! | TTCAGAAACTACAAATAAAA+AGG | - | chr1_4:62487185-62487204 | None:intergenic | 20.0% |
| !! | TTATTTGTAGTTTCTGAATT+AGG | + | chr1_4:62487187-62487206 | Msa0176230:five_prime_UTR | 20.0% |
| !! | TATTTGTAGTTTCTGAATTA+GGG | + | chr1_4:62487188-62487207 | Msa0176230:five_prime_UTR | 20.0% |
| !! | TTCATAACTTTCTTATGAAA+TGG | + | chr1_4:62487989-62488008 | Msa0176230:intron | 20.0% |
| !! | TCATTGGATAATTATATCAT+TGG | + | chr1_4:62488033-62488052 | Msa0176230:intron | 20.0% |
| !! | TCATTGGATATAATATATCA+TGG | + | chr1_4:62488049-62488068 | Msa0176230:intron | 20.0% |
| !! | AACTATATGATTGTTTATGA+TGG | + | chr1_4:62488192-62488211 | Msa0176230:intron | 20.0% |
| !!! | TTGTGTATTTGATAATATCA+TGG | - | chr1_4:62488728-62488747 | None:intergenic | 20.0% |
| !!! | ATTTTACTGGAGAATTTTTT+TGG | + | chr1_4:62488961-62488980 | Msa0176230:CDS | 20.0% |
| !! | GATTTCATTATTCTTGTAAA+TGG | - | chr1_4:62489175-62489194 | None:intergenic | 20.0% |
| !!! | ACTTTTAGTAGTTTATTTCT+TGG | + | chr1_4:62489534-62489553 | Msa0176230:CDS | 20.0% |
| !! | TTCTACATTAGTCAAATTAT+TGG | - | chr1_4:62489964-62489983 | None:intergenic | 20.0% |
| !!! | AATGTAGAAAAAACTTTTGA+TGG | + | chr1_4:62489975-62489994 | Msa0176230:three_prime_UTR | 20.0% |
| !!! | CAACACATTTAATTTTGATA+CGG | - | chr1_4:62490074-62490093 | None:intergenic | 20.0% |
| ! | ACTACAAATAAAAAGGAACA+AGG | - | chr1_4:62487178-62487197 | None:intergenic | 25.0% |
| ! | AGAAAAAAGAAATACCTTCA+TGG | - | chr1_4:62487866-62487885 | None:intergenic | 25.0% |
| ! | AAAATGCAATATGCATTTGA+AGG | + | chr1_4:62487908-62487927 | Msa0176230:intron | 25.0% |
| ! | AAATGCAATATGCATTTGAA+GGG | + | chr1_4:62487909-62487928 | Msa0176230:intron | 25.0% |
| ! | CTTATTCTAATAAGTGTGAT+CGG | - | chr1_4:62488108-62488127 | None:intergenic | 25.0% |
| ! | CATGATAAACGTTTATGTAT+AGG | - | chr1_4:62488497-62488516 | None:intergenic | 25.0% |
| !! | AGAATACTCTTCATAGTTTT+GGG | - | chr1_4:62488586-62488605 | None:intergenic | 25.0% |
| ! | AATAACATGAATAACCTACA+AGG | - | chr1_4:62488662-62488681 | None:intergenic | 25.0% |
| ! | TGATAATATCATGGAAAATG+AGG | - | chr1_4:62488719-62488738 | None:intergenic | 25.0% |
| !! | GGACTTTTCATAAATTTTAC+TGG | + | chr1_4:62488948-62488967 | Msa0176230:CDS | 25.0% |
| ! | GAATAATAACTCTTAGCATA+AGG | - | chr1_4:62489019-62489038 | None:intergenic | 25.0% |
| !!! | AAGTGATAAACTTTTTGGTT+GGG | - | chr1_4:62489055-62489074 | None:intergenic | 25.0% |
| ! | AACAATAGAAAATAGAGAGT+GGG | + | chr1_4:62489348-62489367 | Msa0176230:CDS | 25.0% |
| !! | GCATTATTTTCTATTTCGTT+TGG | + | chr1_4:62489634-62489653 | Msa0176230:CDS | 25.0% |
| !!! | TTTTAGGCATCTAAAATATG+AGG | + | chr1_4:62489852-62489871 | Msa0176230:CDS | 25.0% |
| ! | CAAATTATTGGCAAACAAAA+AGG | - | chr1_4:62489952-62489971 | None:intergenic | 25.0% |
| ! | CGTATCAAAATTAAATGTGT+TGG | + | chr1_4:62490072-62490091 | Msa0176230:three_prime_UTR | 25.0% |
| AACTATGCAAATCAGTTCAA+TGG | + | chr1_4:62487258-62487277 | Msa0176230:five_prime_UTR | 30.0% | |
| AAGATGAAGAACTTCCTAAT+CGG | + | chr1_4:62487393-62487412 | Msa0176230:exon | 30.0% | |
| TAAAACTCCTGAAAGATTTG+AGG | + | chr1_4:62487490-62487509 | Msa0176230:CDS | 30.0% | |
| AAAACTCCTGAAAGATTTGA+GGG | + | chr1_4:62487491-62487510 | Msa0176230:CDS | 30.0% | |
| GGAAGAAGAATTGATATAAG+AGG | + | chr1_4:62487516-62487535 | Msa0176230:CDS | 30.0% | |
| ! | TTCAAAATTTTCCTTCCAAC+TGG | + | chr1_4:62487594-62487613 | Msa0176230:CDS | 30.0% |
| AAAAGTAGTAACTAACCAGT+TGG | - | chr1_4:62487612-62487631 | None:intergenic | 30.0% | |
| TAACATCATGAACAACTACA+TGG | - | chr1_4:62487815-62487834 | None:intergenic | 30.0% | |
| AATGCAATATGCATTTGAAG+GGG | + | chr1_4:62487910-62487929 | Msa0176230:intron | 30.0% | |
| GCTATTACTATATGTCTCAT+TGG | + | chr1_4:62488017-62488036 | Msa0176230:intron | 30.0% | |
| ! | TTATGATGGAAAAGTACACA+AGG | + | chr1_4:62488206-62488225 | Msa0176230:intron | 30.0% |
| TAGAAGTTGAATCGTACTTT+AGG | + | chr1_4:62488256-62488275 | Msa0176230:intron | 30.0% | |
| TGAAATAAGTCAATCCAAAC+AGG | - | chr1_4:62488283-62488302 | None:intergenic | 30.0% | |
| ! | GAGAATACTCTTCATAGTTT+TGG | - | chr1_4:62488587-62488606 | None:intergenic | 30.0% |
| AATAACCTACAAGGTGATAA+GGG | - | chr1_4:62488653-62488672 | None:intergenic | 30.0% | |
| ! | ACTATCCTTGTTGTGATAAA+AGG | + | chr1_4:62488806-62488825 | Msa0176230:intron | 30.0% |
| ! | CTATCCTTGTTGTGATAAAA+GGG | + | chr1_4:62488807-62488826 | Msa0176230:intron | 30.0% |
| ! | TTTTCCCTTTTATCACAACA+AGG | - | chr1_4:62488814-62488833 | None:intergenic | 30.0% |
| TGTTGTGATAAAAGGGAAAA+AGG | + | chr1_4:62488814-62488833 | Msa0176230:intron | 30.0% | |
| GTGATATCAAACATATCCAA+AGG | - | chr1_4:62488869-62488888 | None:intergenic | 30.0% | |
| ATGAAGAAGTAATGGTTCTT+GGG | - | chr1_4:62488990-62489009 | None:intergenic | 30.0% | |
| TATGAAGAAGTAATGGTTCT+TGG | - | chr1_4:62488991-62489010 | None:intergenic | 30.0% | |
| !! | CAAGTGATAAACTTTTTGGT+TGG | - | chr1_4:62489056-62489075 | None:intergenic | 30.0% |
| CAACCAAAAAGTTTATCACT+TGG | + | chr1_4:62489054-62489073 | Msa0176230:CDS | 30.0% | |
| ! | TCTCCAAGTGATAAACTTTT+TGG | - | chr1_4:62489060-62489079 | None:intergenic | 30.0% |
| AATATGTAACATAGCTAGAG+CGG | - | chr1_4:62489099-62489118 | None:intergenic | 30.0% | |
| AGCTATGTTACATATTCTAG+CGG | + | chr1_4:62489103-62489122 | Msa0176230:CDS | 30.0% | |
| CAACAATAGAAAATAGAGAG+TGG | + | chr1_4:62489347-62489366 | Msa0176230:CDS | 30.0% | |
| AATATATGTCATCACTAACG+AGG | + | chr1_4:62489399-62489418 | Msa0176230:CDS | 30.0% | |
| ATCACAAATAACCAACCTAA+GGG | - | chr1_4:62489509-62489528 | None:intergenic | 30.0% | |
| TTTCAGTCTGACATAATTCA+TGG | - | chr1_4:62489599-62489618 | None:intergenic | 30.0% | |
| TTATGTCAGACTGAAAACTT+TGG | + | chr1_4:62489603-62489622 | Msa0176230:CDS | 30.0% | |
| TATGTCAGACTGAAAACTTT+GGG | + | chr1_4:62489604-62489623 | Msa0176230:CDS | 30.0% | |
| ACAATATGTAGCGTTTATTC+AGG | + | chr1_4:62489742-62489761 | Msa0176230:CDS | 30.0% | |
| CAATATGTAGCGTTTATTCA+GGG | + | chr1_4:62489743-62489762 | Msa0176230:CDS | 30.0% | |
| AATATGTAGCGTTTATTCAG+GGG | + | chr1_4:62489744-62489763 | Msa0176230:CDS | 30.0% | |
| ! | ATTGTACATTGTGTTACTCT+TGG | + | chr1_4:62489905-62489924 | Msa0176230:three_prime_UTR | 30.0% |
| AGAAATAAATCGAGTGAAGG+AGG | - | chr1_4:62487297-62487316 | None:intergenic | 35.0% | |
| CCAAGAAATAAATCGAGTGA+AGG | - | chr1_4:62487300-62487319 | None:intergenic | 35.0% | |
| ! | CCTTCACTCGATTTATTTCT+TGG | + | chr1_4:62487297-62487316 | Msa0176230:five_prime_UTR | 35.0% |
| AGAGTGAAGAAGCTGTTTAA+AGG | + | chr1_4:62487549-62487568 | Msa0176230:CDS | 35.0% | |
| ! | AGGACATACAGATTTAGTTC+TGG | + | chr1_4:62487569-62487588 | Msa0176230:CDS | 35.0% |
| !! | GGACATACAGATTTAGTTCT+GGG | + | chr1_4:62487570-62487589 | Msa0176230:CDS | 35.0% |
| TGAACAAATGTGAAGTGCAA+TGG | - | chr1_4:62487636-62487655 | None:intergenic | 35.0% | |
| CTTATCTACAAACGTAAGCT+TGG | + | chr1_4:62488304-62488323 | Msa0176230:intron | 35.0% | |
| ! | TGAAGACATTTAGGGTTGTT+TGG | - | chr1_4:62488533-62488552 | None:intergenic | 35.0% |
| GATCTACGTGAAGACATTTA+GGG | - | chr1_4:62488541-62488560 | None:intergenic | 35.0% | |
| AGATCTACGTGAAGACATTT+AGG | - | chr1_4:62488542-62488561 | None:intergenic | 35.0% | |
| AATGTCTTCACGTAGATCTT+TGG | + | chr1_4:62488543-62488562 | Msa0176230:intron | 35.0% | |
| TAAAACCCTTATCACCTTGT+AGG | + | chr1_4:62488645-62488664 | Msa0176230:intron | 35.0% | |
| GAATAACCTACAAGGTGATA+AGG | - | chr1_4:62488654-62488673 | None:intergenic | 35.0% | |
| AAAAGGGAAAAAGGTGAAGA+AGG | + | chr1_4:62488823-62488842 | Msa0176230:intron | 35.0% | |
| AGAGAACTATGAACTTCCTT+TGG | + | chr1_4:62488850-62488869 | Msa0176230:CDS | 35.0% | |
| GGCGATATATGAAGAAGTAA+TGG | - | chr1_4:62488998-62489017 | None:intergenic | 35.0% | |
| TTGGTTGGGTATGCTAATAA+AGG | - | chr1_4:62489041-62489060 | None:intergenic | 35.0% | |
| AGTTTATCACTTGGAGATGA+TGG | + | chr1_4:62489063-62489082 | Msa0176230:CDS | 35.0% | |
| TAAAGAAGAGTTACCTTCAG+AGG | - | chr1_4:62489209-62489228 | None:intergenic | 35.0% | |
| GTCTATCAATCTCGAAATTG+TGG | + | chr1_4:62489310-62489329 | Msa0176230:CDS | 35.0% | |
| ATCAATCTCGAAATTGTGGA+TGG | + | chr1_4:62489314-62489333 | Msa0176230:CDS | 35.0% | |
| TCAATCTCGAAATTGTGGAT+GGG | + | chr1_4:62489315-62489334 | Msa0176230:CDS | 35.0% | |
| CATCACAAATAACCAACCTA+AGG | - | chr1_4:62489510-62489529 | None:intergenic | 35.0% | |
| GTACACATTTGCACTTTCAT+TGG | - | chr1_4:62489560-62489579 | None:intergenic | 35.0% | |
| AGCAACTCTGTGTATTACAT+TGG | + | chr1_4:62489709-62489728 | Msa0176230:CDS | 35.0% | |
| GATGAGATTCAAGAAGAGAT+GGG | - | chr1_4:62489801-62489820 | None:intergenic | 35.0% | |
| GAATCTCATCCTACAATTGT+TGG | + | chr1_4:62489811-62489830 | Msa0176230:CDS | 35.0% | |
| TAGATGCCTAAAACACCAAT+CGG | - | chr1_4:62489845-62489864 | None:intergenic | 35.0% | |
| AATCGAGTGAAGGAGGATTA+AGG | - | chr1_4:62487290-62487309 | None:intergenic | 40.0% | |
| TAATCGGCCACGATAAACTA+GGG | + | chr1_4:62487409-62487428 | Msa0176230:CDS | 40.0% | |
| TGAGAAACCCTAGTTTATCG+TGG | - | chr1_4:62487419-62487438 | None:intergenic | 40.0% | |
| CGATAAACTAGGGTTTCTCA+AGG | + | chr1_4:62487419-62487438 | Msa0176230:CDS | 40.0% | |
| GTAGTAACTAACCAGTTGGA+AGG | - | chr1_4:62487608-62487627 | None:intergenic | 40.0% | |
| TGTATTGCTGTGATCCATGA+AGG | + | chr1_4:62487849-62487868 | Msa0176230:intron | 40.0% | |
| !! | ATCGTACTTTAGGACCTGTT+TGG | + | chr1_4:62488266-62488285 | Msa0176230:intron | 40.0% |
| ! | GACGACCTATTTCAGTTTTC+CGG | + | chr1_4:62488908-62488927 | Msa0176230:CDS | 40.0% |
| AAGAGTTACCTTCAGAGGTA+GGG | - | chr1_4:62489204-62489223 | None:intergenic | 40.0% | |
| ! | GCATTTGCTAAAAGCAAGCA+AGG | - | chr1_4:62489242-62489261 | None:intergenic | 40.0% |
| ! | TGCTTTTAGCAAATGCTCTG+AGG | + | chr1_4:62489246-62489265 | Msa0176230:CDS | 40.0% |
| ! | CTCTGAGGAATTTGTCTTTG+TGG | + | chr1_4:62489261-62489280 | Msa0176230:CDS | 40.0% |
| GAACTAGGAATTCCTATGAC+GGG | - | chr1_4:62489487-62489506 | None:intergenic | 40.0% | |
| GGAACTAGGAATTCCTATGA+CGG | - | chr1_4:62489488-62489507 | None:intergenic | 40.0% | |
| TAGGAATTCCTAGTTCCCTT+AGG | + | chr1_4:62489491-62489510 | Msa0176230:CDS | 40.0% | |
| AATTCCTAGTTCCCTTAGGT+TGG | + | chr1_4:62489495-62489514 | Msa0176230:CDS | 40.0% | |
| ATAACCAACCTAAGGGAACT+AGG | - | chr1_4:62489502-62489521 | None:intergenic | 40.0% | |
| GGATGAGATTCAAGAAGAGA+TGG | - | chr1_4:62489802-62489821 | None:intergenic | 40.0% | |
| CTCATCCTACAATTGTTGGT+TGG | + | chr1_4:62489815-62489834 | Msa0176230:CDS | 40.0% | |
| CTAGTTTATCGTGGCCGATT+AGG | - | chr1_4:62487410-62487429 | None:intergenic | 45.0% | |
| CTAATCGGCCACGATAAACT+AGG | + | chr1_4:62487408-62487427 | Msa0176230:CDS | 45.0% | |
| TTCCAGCCCTCAAATCTTTC+AGG | - | chr1_4:62487500-62487519 | None:intergenic | 45.0% | |
| ACACGCCGGAAAACTGAAAT+AGG | - | chr1_4:62488916-62488935 | None:intergenic | 45.0% | |
| AGAGTTACCTTCAGAGGTAG+GGG | - | chr1_4:62489203-62489222 | None:intergenic | 45.0% | |
| GAAGAGTTACCTTCAGAGGT+AGG | - | chr1_4:62489205-62489224 | None:intergenic | 45.0% | |
| !! | AGAGTGGGTTGTTGACTTTG+TGG | + | chr1_4:62489363-62489382 | Msa0176230:CDS | 45.0% |
| ATCACTAACGAGGCGAAGAT+AGG | + | chr1_4:62489409-62489428 | Msa0176230:CDS | 45.0% | |
| TCACTAACGAGGCGAAGATA+GGG | + | chr1_4:62489410-62489429 | Msa0176230:CDS | 45.0% | |
| ATTGAGCAACCCGAACAAGT+GGG | + | chr1_4:62489678-62489697 | Msa0176230:CDS | 45.0% | |
| TCTCATATCCCCACTTGTTC+GGG | - | chr1_4:62489690-62489709 | None:intergenic | 45.0% | |
| CTCTCATATCCCCACTTGTT+CGG | - | chr1_4:62489691-62489710 | None:intergenic | 45.0% | |
| GCGTTCCAACCAACAATTGT+AGG | - | chr1_4:62489823-62489842 | None:intergenic | 45.0% | |
| CACTCGAATCATCCCTTCCA+CGG | + | chr1_4:62487225-62487244 | Msa0176230:five_prime_UTR | 50.0% | |
| CTCCTGAAAGATTTGAGGGC+TGG | + | chr1_4:62487495-62487514 | Msa0176230:CDS | 50.0% | |
| TTTCCGGCGTGTGCAAGAAT+TGG | + | chr1_4:62488924-62488943 | Msa0176230:CDS | 50.0% | |
| TCAATTCCCCCTACCTCTGA+AGG | + | chr1_4:62489193-62489212 | Msa0176230:CDS | 50.0% | |
| GAGTTACCTTCAGAGGTAGG+GGG | - | chr1_4:62489202-62489221 | None:intergenic | 50.0% | |
| CACTAACGAGGCGAAGATAG+GGG | + | chr1_4:62489411-62489430 | Msa0176230:CDS | 50.0% | |
| CATTGAGCAACCCGAACAAG+TGG | + | chr1_4:62489677-62489696 | Msa0176230:CDS | 50.0% | |
| TTGAGCAACCCGAACAAGTG+GGG | + | chr1_4:62489679-62489698 | Msa0176230:CDS | 50.0% | |
| ! | TTGTTGGTTGGAACGCCGAT+TGG | + | chr1_4:62489827-62489846 | Msa0176230:CDS | 50.0% |
| !! | GGAACGCCGATTGGTGTTTT+AGG | + | chr1_4:62489836-62489855 | Msa0176230:CDS | 50.0% |
| TTGAACGAGCAGCCGTGGAA+GGG | - | chr1_4:62487240-62487259 | None:intergenic | 55.0% | |
| CATAGTTGAACGAGCAGCCG+TGG | - | chr1_4:62487245-62487264 | None:intergenic | 55.0% | |
| CCTCCAATTCTTGCACACGC+CGG | - | chr1_4:62488930-62488949 | None:intergenic | 55.0% | |
| !! | CTGAGGAGTACTCCCGTCAT+AGG | + | chr1_4:62489472-62489491 | Msa0176230:CDS | 55.0% |
| GTTGAACGAGCAGCCGTGGA+AGG | - | chr1_4:62487241-62487260 | None:intergenic | 60.0% | |
| CCGGCGTGTGCAAGAATTGG+AGG | + | chr1_4:62488927-62488946 | Msa0176230:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_4 | gene | 62487118 | 62490096 | 62487118 | ID=Msa0176230;Name=Msa0176230 |
| chr1_4 | mRNA | 62487118 | 62490096 | 62487118 | ID=Msa0176230-mRNA-1;Parent=Msa0176230;Name=Msa0176230-mRNA-1;_AED=0.01;_eAED=0.01;_QI=278|1|1|1|1|1|2|199|429 |
| chr1_4 | exon | 62487118 | 62487615 | 62487118 | ID=Msa0176230-mRNA-1:exon:20120;Parent=Msa0176230-mRNA-1 |
| chr1_4 | exon | 62488828 | 62490096 | 62488828 | ID=Msa0176230-mRNA-1:exon:20121;Parent=Msa0176230-mRNA-1 |
| chr1_4 | five_prime_UTR | 62487118 | 62487395 | 62487118 | ID=Msa0176230-mRNA-1:five_prime_utr;Parent=Msa0176230-mRNA-1 |
| chr1_4 | CDS | 62487396 | 62487615 | 62487396 | ID=Msa0176230-mRNA-1:cds;Parent=Msa0176230-mRNA-1 |
| chr1_4 | CDS | 62488828 | 62489897 | 62488828 | ID=Msa0176230-mRNA-1:cds;Parent=Msa0176230-mRNA-1 |
| chr1_4 | three_prime_UTR | 62489898 | 62490096 | 62489898 | ID=Msa0176230-mRNA-1:three_prime_utr;Parent=Msa0176230-mRNA-1 |
| Gene Sequence |
| Protein sequence |
