AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0084880

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0084880 MtrunA17_Chr1g0199681 96.800 625 13 3 1 624 76 694 0.0 1249
Msa0084880 MtrunA17_Chr2g0326761 58.115 573 198 12 10 574 2 540 0.0 608
Msa0084880 MtrunA17_Chr4g0028971 57.931 580 208 15 8 576 1 555 0.0 572
Msa0084880 MtrunA17_Chr1g0181211 53.299 591 213 14 13 578 21 573 0.0 561
Msa0084880 MtrunA17_Chr7g0264171 48.710 620 263 16 10 613 22 602 2.42e-176 514
Msa0084880 MtrunA17_Chr7g0264161 54.706 340 111 4 28 363 1 301 1.58e-124 370
Msa0084880 MtrunA17_Chr3g0115271 38.562 612 287 17 16 595 16 570 2.91e-113 351
Msa0084880 MtrunA17_Chr5g0424931 37.736 583 268 22 16 579 16 522 2.41e-99 313
Msa0084880 MtrunA17_Chr5g0417281 37.712 472 215 13 11 464 3 413 2.04e-87 281
Msa0084880 MtrunA17_Chr5g0433301 39.286 392 190 8 9 391 14 366 1.20e-80 271
Msa0084880 MtrunA17_Chr2g0282961 39.474 380 176 8 15 383 51 387 5.45e-80 265
Msa0084880 MtrunA17_Chr4g0070181 40.157 381 180 9 13 385 17 357 5.84e-80 274
Msa0084880 MtrunA17_Chr5g0436761 35.504 476 231 12 8 473 1 410 4.71e-79 258
Msa0084880 MtrunA17_Chr2g0285461 38.107 412 208 8 9 412 13 385 1.17e-78 267
Msa0084880 MtrunA17_Chr3g0109391 38.918 388 189 8 13 391 20 368 1.44e-77 263
Msa0084880 MtrunA17_Chr2g0289221 39.440 393 169 10 16 393 17 355 2.44e-77 250
Msa0084880 MtrunA17_Chr8g0376771 37.713 411 210 8 9 412 13 384 3.80e-77 263
Msa0084880 MtrunA17_Chr4g0046371 39.844 384 179 10 12 383 33 376 6.35e-77 258
Msa0084880 MtrunA17_Chr1g0178011 36.750 400 200 7 14 407 7 359 1.62e-76 251
Msa0084880 MtrunA17_Chr1g0156321 38.750 400 192 11 14 403 38 394 1.22e-74 256
Msa0084880 MtrunA17_Chr8g0385791 37.469 403 203 8 10 404 50 411 2.32e-72 249
Msa0084880 MtrunA17_Chr5g0417211 39.142 373 155 9 16 373 9 324 6.83e-71 234
Msa0084880 MtrunA17_Chr8g0389081 38.660 388 188 8 9 386 22 369 3.98e-70 246
Msa0084880 MtrunA17_Chr4g0029671 37.440 414 180 10 19 404 54 416 4.08e-70 242
Msa0084880 MtrunA17_Chr2g0301251 39.578 379 183 11 14 385 18 357 1.89e-69 244
Msa0084880 MtrunA17_Chr2g0277461 37.437 398 187 14 19 407 13 357 2.71e-69 232
Msa0084880 MtrunA17_Chr2g0326281 37.346 407 182 9 19 393 56 421 4.34e-69 239
Msa0084880 MtrunA17_Chr4g0009751 37.406 401 196 10 15 404 15 371 3.57e-68 233
Msa0084880 MtrunA17_Chr2g0276781 36.869 396 195 7 11 395 15 366 7.12e-68 233
Msa0084880 MtrunA17_Chr7g0239061 35.854 410 205 12 8 405 3 366 1.36e-61 216
Msa0084880 MtrunA17_Chr8g0346711 37.688 398 195 10 16 404 26 379 6.85e-61 214
Msa0084880 MtrunA17_Chr5g0425261 35.417 384 186 14 16 389 9 340 1.88e-56 195
Msa0084880 MtrunA17_Chr5g0424801 34.828 379 186 13 16 384 9 336 6.48e-56 194
Msa0084880 MtrunA17_Chr2g0277531 33.894 357 166 12 19 363 20 318 5.79e-52 181
Msa0084880 MtrunA17_Chr5g0425661 42.000 200 95 4 18 206 36 225 7.35e-47 165
Msa0084880 MtrunA17_Chr5g0424741 30.693 505 247 19 16 461 9 469 1.13e-46 172
Msa0084880 MtrunA17_Chr8g0356211 31.529 314 163 8 126 439 2 263 5.51e-37 140
Msa0084880 MtrunA17_Chr5g0424791 35.526 152 87 4 63 214 11 151 2.23e-20 88.2
Msa0084880 MtrunA17_Chr5g0425651 41.739 115 60 3 277 387 1 112 8.52e-20 85.5
Msa0084880 MtrunA17_Chr1g0178071 35.780 109 67 2 113 218 71 179 1.75e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0084880 AT4G30080.1 53.734 616 230 16 1 603 1 574 0.0 585
Msa0084880 AT2G28350.1 55.690 580 196 16 13 567 4 547 0.0 564
Msa0084880 AT2G28350.2 55.690 580 196 16 13 567 4 547 0.0 564
Msa0084880 AT1G77850.1 38.399 612 303 17 16 599 16 581 3.24e-118 364
Msa0084880 AT1G77850.2 38.399 612 303 17 16 599 16 581 5.57e-118 363
Msa0084880 AT5G37020.2 38.918 388 189 8 13 391 16 364 1.48e-79 268
Msa0084880 AT5G37020.1 38.918 388 189 8 13 391 16 364 4.87e-79 267
Msa0084880 AT1G30330.3 38.517 418 207 10 9 416 14 391 5.72e-78 266
Msa0084880 AT1G30330.2 38.517 418 207 10 9 416 14 391 5.72e-78 266
Msa0084880 AT1G30330.1 38.015 413 207 9 13 416 17 389 1.41e-77 265
Msa0084880 AT1G19850.2 39.119 386 179 9 11 383 35 377 1.14e-74 256
Msa0084880 AT1G19220.1 38.480 408 193 12 13 409 17 377 1.16e-74 259
Msa0084880 AT1G19850.1 39.119 386 179 9 11 383 46 388 1.50e-74 256
Msa0084880 AT2G33860.1 38.636 396 193 9 15 401 52 406 4.51e-74 249
Msa0084880 AT5G60450.2 36.650 412 201 7 8 401 30 399 5.33e-74 252
Msa0084880 AT5G60450.1 37.065 402 193 7 18 401 64 423 6.49e-73 250
Msa0084880 AT5G62000.2 38.155 401 197 8 13 404 55 413 9.36e-72 248
Msa0084880 AT5G62000.5 38.155 401 197 8 13 404 55 413 9.36e-72 248
Msa0084880 AT5G62000.3 38.155 401 197 8 13 404 55 413 9.36e-72 248
Msa0084880 AT5G62000.1 38.155 401 197 8 13 404 55 413 9.36e-72 248
Msa0084880 AT5G62000.4 38.155 401 197 8 13 404 55 413 1.49e-71 247
Msa0084880 AT5G20730.2 33.397 527 271 16 13 507 18 496 1.70e-70 248
Msa0084880 AT5G20730.1 33.523 528 270 17 13 507 18 497 2.97e-69 244
Msa0084880 AT5G20730.3 33.523 528 270 17 13 507 18 497 4.20e-69 244
Msa0084880 AT1G59750.4 38.095 399 197 8 15 404 18 375 1.95e-67 233
Msa0084880 AT1G59750.2 38.095 399 197 8 15 404 18 375 2.11e-67 232
Msa0084880 AT1G59750.1 38.095 399 197 8 15 404 18 375 2.30e-67 232
Msa0084880 AT1G59750.3 38.095 399 197 8 15 404 18 375 2.30e-67 232
Msa0084880 AT2G46530.1 39.211 380 172 13 16 383 18 350 4.09e-66 228
Msa0084880 AT2G46530.3 39.211 380 172 13 16 383 39 371 9.24e-66 227
Msa0084880 AT3G61830.1 36.675 409 197 11 7 404 13 370 2.34e-65 226
Msa0084880 AT4G23980.2 37.313 402 199 11 16 408 9 366 2.17e-64 224
Msa0084880 AT4G23980.1 37.313 402 199 11 16 408 9 366 2.33e-64 224
Msa0084880 AT5G37020.3 37.662 308 151 6 86 391 1 269 5.49e-55 199
Msa0084880 AT1G35520.1 35.897 390 193 12 12 393 20 360 1.91e-54 196
Msa0084880 AT1G34310.1 36.292 383 187 11 19 393 27 360 2.20e-54 195
Msa0084880 AT1G35540.1 35.038 391 203 9 12 396 20 365 5.06e-54 194
Msa0084880 AT3G61830.2 37.536 349 163 10 60 404 22 319 8.51e-54 193
Msa0084880 AT1G35240.1 35.433 381 189 12 12 383 20 352 8.92e-54 194
Msa0084880 AT1G34390.1 34.368 419 209 14 12 422 20 380 4.38e-53 192
Msa0084880 AT2G33860.2 38.732 284 138 4 118 401 2 249 1.68e-52 187
Msa0084880 AT1G34170.1 33.503 394 203 12 19 402 25 369 2.34e-52 188
Msa0084880 AT1G34410.1 35.000 380 192 11 12 383 20 352 4.99e-52 189
Msa0084880 AT2G46530.2 40.370 270 116 8 117 383 36 263 6.11e-48 176
Msa0084880 AT1G34170.2 31.980 394 183 12 19 402 25 343 4.05e-45 167
Msa0084880 AT1G34170.3 31.980 394 183 12 19 402 25 343 3.09e-44 166
Msa0084880 AT1G43950.1 40.654 214 108 7 12 217 20 222 3.43e-38 141
Msa0084880 ATMG00940.1 37.736 159 83 5 205 358 1 148 3.06e-23 97.1
Msa0084880 AT2G36080.3 35.714 112 66 3 112 218 32 142 1.37e-11 65.5
Msa0084880 AT2G36080.1 34.234 111 69 2 112 218 32 142 1.55e-11 65.5
Msa0084880 AT2G36080.4 34.234 111 69 2 112 218 32 142 2.65e-11 63.2
Msa0084880 AT2G36080.2 34.234 111 69 2 112 218 32 142 2.65e-11 63.2
Msa0084880 AT3G25730.1 32.824 131 76 2 118 246 183 303 4.60e-11 65.1
Msa0084880 AT2G46870.1 34.906 106 62 2 110 215 27 125 6.92e-11 64.3

Find 144 sgRNAs with CRISPR-Local

Find 196 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTAGGATTAGTTGGTTTA+TGG 0.157206 1_2:+64586867 Msa0084880:CDS
TTTGAAGAAGATTGTTTCTT+TGG 0.183552 1_2:+64586163 Msa0084880:CDS
ACAAACCTTGGTTTCTTTCT+TGG 0.220376 1_2:-64587704 None:intergenic
AAATCAGATGGATTATTATT+TGG 0.221410 1_2:-64587905 None:intergenic
TATTGCATAATTCTTCTATT+AGG 0.232046 1_2:+64588480 Msa0084880:three_prime_UTR
ACAAAATATGGTTATGGTTA+TGG 0.233884 1_2:-64588147 None:intergenic
GAGATCTTTGTGTTGGTATA+AGG 0.249926 1_2:+64586500 Msa0084880:CDS
ACGTAGACTTCATCAGTTTC+AGG 0.253211 1_2:-64586100 None:intergenic
GAGAGGTGGAACACGGGTTT+TGG 0.257788 1_2:-64586038 None:intergenic
TGATGCAAAAGAACTATTAT+TGG 0.261306 1_2:-64587849 None:intergenic
CTACAACAAGACATGTTTCT+TGG 0.267352 1_2:+64587947 Msa0084880:CDS
TTCTCCTTGGCGCCTTCTTC+AGG 0.267828 1_2:+64586936 Msa0084880:CDS
TTGTTGAGTGTGGGAAATTC+TGG 0.282323 1_2:+64588085 Msa0084880:CDS
CAAAATATGGTTATGGTTAT+GGG 0.304272 1_2:-64588146 None:intergenic
GGAGCCAGGCATGCTCAATT+TGG 0.304628 1_2:+64587881 Msa0084880:CDS
TTGAAACTGAGGATTCTTCT+AGG 0.307810 1_2:+64586851 Msa0084880:CDS
ATTGTTATTGATACTCTTGT+AGG 0.312189 1_2:-64588029 None:intergenic
TCTAGGATTAGTTGGTTTAT+GGG 0.316818 1_2:+64586868 Msa0084880:CDS
CCATAAGGAGGCCTAGTTAT+AGG 0.329666 1_2:-64588004 None:intergenic
AATAGAAGAATTATGCAATA+TGG 0.330857 1_2:-64588477 None:intergenic
CACCATTGTTTGCATCAGAT+TGG 0.343534 1_2:-64586240 None:intergenic
CAAATAACAAAATATGGTTA+TGG 0.347669 1_2:-64588153 None:intergenic
TGATTTATGAGGTGAAAATA+TGG 0.352018 1_2:-64587734 None:intergenic
GCTGTAAACTGTGCTGTTAA+TGG 0.368748 1_2:+64586706 Msa0084880:CDS
TGGAGGCTCTCCTTGGTATA+AGG 0.368842 1_2:+64588297 Msa0084880:CDS
GTGTGGTTGGATGAGGAAAA+AGG 0.376961 1_2:-64585802 None:intergenic
CCTATAACTAGGCCTCCTTA+TGG 0.378633 1_2:+64588004 Msa0084880:CDS
TTCTTTCTTGGAGGTGTGAA+TGG 0.380455 1_2:-64587692 None:intergenic
TTTCTAATTCTTCTAGTACT+TGG 0.397904 1_2:+64586563 Msa0084880:CDS
ATGCAAATTCAATGGTGTTC+TGG 0.400255 1_2:+64586808 Msa0084880:CDS
GGAGAATCAGGCCAACGAAT+TGG 0.402668 1_2:-64586919 None:intergenic
CTTTGGAAACAATGGCTTAG+AGG 0.405587 1_2:+64586180 Msa0084880:CDS
AGGATTCTTCTAGGATTAGT+TGG 0.407748 1_2:+64586860 Msa0084880:CDS
TTCGTTGGCCTGATTCTCCT+TGG 0.409284 1_2:+64586923 Msa0084880:CDS
TTAGCAATAATTGTTTGAAC+AGG 0.414237 1_2:-64586331 None:intergenic
AGAGCAAAGAAAGGTGGAAT+TGG 0.414735 1_2:+64586523 Msa0084880:CDS
ACCATTGTTTGCATCAGATT+GGG 0.415921 1_2:-64586239 None:intergenic
AGAAGGCGCCAAGGAGAATC+AGG 0.419546 1_2:-64586931 None:intergenic
AAATGGTGGTAATGATTTGA+TGG 0.424677 1_2:+64586654 Msa0084880:CDS
CAACACAAGCCTGATCTTGT+TGG 0.424795 1_2:+64588325 Msa0084880:CDS
AACCTTGGTTTCTTTCTTGG+AGG 0.432289 1_2:-64587701 None:intergenic
AAACCCTTTGATGAACTAAA+GGG 0.434794 1_2:+64588575 Msa0084880:three_prime_UTR
CCTTTGTTTGGTGGTGGTGG+TGG 0.436011 1_2:+64586598 Msa0084880:CDS
CCATCAGAAGAATTCTCAAC+TGG 0.442393 1_2:-64588277 None:intergenic
GAAACCTTAACACAAAATTC+AGG 0.450898 1_2:-64586769 None:intergenic
TTAAGAATAATTAGCTAGCT+AGG 0.455608 1_2:-64588417 None:intergenic
TAAACCCTTTGATGAACTAA+AGG 0.456993 1_2:+64588574 Msa0084880:three_prime_UTR
ATAGTCTCAGCACAGTATCT+AGG 0.458408 1_2:-64586277 None:intergenic
TGGTCCAAATTGAGCATGCC+TGG 0.458791 1_2:-64587885 None:intergenic
CTTCTGATGGAGGCTCTCCT+TGG 0.459576 1_2:+64588290 Msa0084880:CDS
CGTAGACTTCATCAGTTTCA+GGG 0.459745 1_2:-64586099 None:intergenic
AGATTAATGTTGGAGATTGA+TGG 0.460974 1_2:-64587782 None:intergenic
TCACCTTTGTTTGGTGGTGG+TGG 0.463200 1_2:+64586595 Msa0084880:CDS
ATGAATGAATTTCTATATCA+AGG 0.463564 1_2:+64588668 Msa0084880:three_prime_UTR
CTTGTCTTGTTTGTTGAGTG+TGG 0.463677 1_2:+64588075 Msa0084880:CDS
AGTGCTTAGATTCACAACTA+TGG 0.464164 1_2:+64585912 Msa0084880:CDS
AATGTCTTTGCAAAAGAAGC+TGG 0.466988 1_2:-64586214 None:intergenic
AACCGTGTATCACCTTTGTT+TGG 0.470136 1_2:+64586586 Msa0084880:CDS
TTGTTATTGATACTCTTGTA+GGG 0.470816 1_2:-64588028 None:intergenic
AAATGTTACTGCATTGTGTA+TGG 0.470847 1_2:+64588354 Msa0084880:CDS
TTGAAGGGAATTAAATCAGA+TGG 0.471043 1_2:-64587917 None:intergenic
GCAATAATTGTTTGAACAGG+TGG 0.472648 1_2:-64586328 None:intergenic
ATAATTGAGAAGATTAATGT+TGG 0.477845 1_2:-64587792 None:intergenic
AAGTTTAGACATATCTATAG+AGG 0.484340 1_2:+64586376 Msa0084880:CDS
AATGGTGAGAGATTGAAGTT+TGG 0.485606 1_2:-64587674 None:intergenic
TACCCCTTTAGTTCATCAAA+GGG 0.485698 1_2:-64588578 None:intergenic
GAAAATTCCTCTTCCACTTG+TGG 0.485724 1_2:+64588633 Msa0084880:three_prime_UTR
CATAATTCCACAAGTGGAAG+AGG 0.488384 1_2:-64588640 None:intergenic
AATCACAAGAAGCTTGTTGC+TGG 0.491007 1_2:+64586445 Msa0084880:CDS
AATCTGCAATGCAAATTCAA+TGG 0.492272 1_2:+64586800 Msa0084880:CDS
ATACTCTTGTAGGGTCCATA+AGG 0.493770 1_2:-64588019 None:intergenic
GAAGAATCCTCAGTTTCAAA+AGG 0.493790 1_2:-64586847 None:intergenic
TTGGTAACTTGTCATTGTCA+AGG 0.495636 1_2:+64587966 Msa0084880:CDS
CATCAAGCATACAGGGAGCC+AGG 0.498276 1_2:+64587867 Msa0084880:CDS
TTGTTGTAGTAGCTTGTTGA+AGG 0.499531 1_2:-64587933 None:intergenic
AATTCAGGAGTACTAGCTCT+TGG 0.500520 1_2:-64586754 None:intergenic
GATTGTTTCTTTGGAAACAA+TGG 0.500964 1_2:+64586172 Msa0084880:CDS
TATGGATCTTGAACAAACCT+TGG 0.501143 1_2:-64587716 None:intergenic
TAACTTGTTTAAGGCAATGA+GGG 0.508770 1_2:+64588611 Msa0084880:three_prime_UTR
GTTTGTGGTTATGGATTCCA+TGG 0.512063 1_2:+64585874 Msa0084880:CDS
CGTGTATCACCTTTGTTTGG+TGG 0.513472 1_2:+64586589 Msa0084880:CDS
TTGTCTTGTTTGTTGAGTGT+GGG 0.513483 1_2:+64588076 Msa0084880:CDS
ATAATATTGTTACCTGAAGA+AGG 0.514309 1_2:-64586948 None:intergenic
CAACTATGGCACGCATGTGC+TGG 0.516615 1_2:+64585926 Msa0084880:CDS
TTTGGAAACAATGGCTTAGA+GGG 0.516902 1_2:+64586181 Msa0084880:CDS
TTACCCCTTTAGTTCATCAA+AGG 0.517930 1_2:-64588579 None:intergenic
GCATTGGAAGTTGATTTATG+AGG 0.518050 1_2:-64587745 None:intergenic
AAGAGTGGTTCTGTTTCTGA+AGG 0.520431 1_2:+64588217 Msa0084880:CDS
AGTTCAAATATTTCAAAGAG+TGG 0.525439 1_2:+64588202 Msa0084880:CDS
AAGATATGCATGGACAGTGT+TGG 0.525936 1_2:+64586353 Msa0084880:CDS
GGTGGTTCTGGTTTCTTGTG+TGG 0.526295 1_2:+64586616 Msa0084880:CDS
GCAAAGAAAGGTGGAATTGG+TGG 0.533246 1_2:+64586526 Msa0084880:CDS
TAATGATTTGATGGGAAAAG+TGG 0.533977 1_2:+64586663 Msa0084880:CDS
CCAGTTGAGAATTCTTCTGA+TGG 0.534523 1_2:+64588277 Msa0084880:CDS
GTATCACCTTTGTTTGGTGG+TGG 0.534706 1_2:+64586592 Msa0084880:CDS
AGAGAAAGTGAAGGTGTGGT+TGG 0.536093 1_2:-64585815 None:intergenic
GAAAATGGAGATCTTTGTGT+TGG 0.538803 1_2:+64586493 Msa0084880:CDS
TTCATAGCAGAGATTCTACA+AGG 0.548674 1_2:-64586064 None:intergenic
TGTTGTTGATCCTTATACCA+AGG 0.550241 1_2:-64588307 None:intergenic
AGTGAAGGTGTGGTTGGATG+AGG 0.550919 1_2:-64585809 None:intergenic
TAGTTACATAATTCCACAAG+TGG 0.556216 1_2:-64588646 None:intergenic
GTTGTCAAAAGATGTCTCCT+TGG 0.557297 1_2:-64586403 None:intergenic
AAGAAAGGTGGAATTGGTGG+TGG 0.559157 1_2:+64586529 Msa0084880:CDS
AAGAGAAAAGAAAAGAAGAG+TGG 0.559701 1_2:-64585771 None:intergenic
GGTGATGATGATGATGGCAT+TGG 0.562514 1_2:-64587761 None:intergenic
TTTAACATTGTGGAATGTGA+AGG 0.564248 1_2:+64588753 Msa0084880:three_prime_UTR
GAGACTAATTCAACTAACCA+AGG 0.569877 1_2:-64587644 None:intergenic
TTAACTTGTTTAAGGCAATG+AGG 0.574265 1_2:+64588610 Msa0084880:three_prime_UTR
GCACGCATGTGCTGGTGCCA+TGG 0.574468 1_2:+64585934 Msa0084880:CDS
ATAAGGAGAGCAAAGAAAGG+TGG 0.575173 1_2:+64586517 Msa0084880:CDS
TGTTGTAGTAGCTTGTTGAA+GGG 0.575894 1_2:-64587932 None:intergenic
GGTATAAGGAGAGCAAAGAA+AGG 0.576664 1_2:+64586514 Msa0084880:CDS
AAAATATGGTTATGGTTATG+GGG 0.588796 1_2:-64588145 None:intergenic
ATTATTGCTAAAGATATGCA+TGG 0.591151 1_2:+64586343 Msa0084880:CDS
ATGTTCAAGATCCAATTCGT+TGG 0.592882 1_2:+64586908 Msa0084880:CDS
GTTGAGAATTCTTCTGATGG+AGG 0.594043 1_2:+64588280 Msa0084880:CDS
GAGATTCTACAAGGGATGAG+AGG 0.595750 1_2:-64586055 None:intergenic
TCTGCTGAATAATCCAACCT+AGG 0.603231 1_2:-64586304 None:intergenic
GCATGTTCTGCATGACCTTG+TGG 0.603875 1_2:-64585995 None:intergenic
AGACTAATTCAACTAACCAA+GGG 0.605653 1_2:-64587643 None:intergenic
ATCATCAACAACCTATAACT+AGG 0.606131 1_2:+64587993 Msa0084880:CDS
ATGTGAAATGTGTAAACCCT+TGG 0.607377 1_2:+64587627 Msa0084880:CDS
AATCTCTGCTATGAAATACA+TGG 0.608031 1_2:+64586072 Msa0084880:CDS
AATGGTGGTAATGATTTGAT+GGG 0.610306 1_2:+64586655 Msa0084880:CDS
TTAACATTGTGGAATGTGAA+GGG 0.613741 1_2:+64588754 Msa0084880:three_prime_UTR
AAGGGATGAGAGGTGGAACA+CGG 0.623921 1_2:-64586045 None:intergenic
CACCAAACAAAGGTGATACA+CGG 0.628457 1_2:-64586588 None:intergenic
ACCCAATCTGATGCAAACAA+TGG 0.629763 1_2:+64586238 Msa0084880:CDS
CTCTTGTAGGGTCCATAAGG+AGG 0.630872 1_2:-64588016 None:intergenic
ATTCTACAAGGGATGAGAGG+TGG 0.633006 1_2:-64586052 None:intergenic
AATGATTTGATGGGAAAAGT+GGG 0.635170 1_2:+64586664 Msa0084880:CDS
ATTGATGGTGATGATGATGA+TGG 0.637177 1_2:-64587767 None:intergenic
CACCTCCAAGAAAGAAACCA+AGG 0.638580 1_2:+64587699 Msa0084880:CDS
AACCCTTTGATGAACTAAAG+GGG 0.646968 1_2:+64588576 Msa0084880:three_prime_UTR
GTTACCTGAAGAAGGCGCCA+AGG 0.651106 1_2:-64586940 None:intergenic
GAGTGGTGGCATTTGAACCA+TGG 0.651177 1_2:-64585951 None:intergenic
TCTGATGCAAACAATGGTGG+TGG 0.656065 1_2:+64586244 Msa0084880:CDS
AGGGATGAGAGGTGGAACAC+GGG 0.668889 1_2:-64586044 None:intergenic
AAAGTCTTCTACTTTCCACA+AGG 0.669890 1_2:+64585980 Msa0084880:CDS
TCATAGCAGAGATTCTACAA+GGG 0.672161 1_2:-64586063 None:intergenic
GTGAAGAGAAAGTGAAGGTG+TGG 0.672989 1_2:-64585819 None:intergenic
CAATCTGATGCAAACAATGG+TGG 0.688240 1_2:+64586241 Msa0084880:CDS
TTGTTGTGAAGAGAAAGTGA+AGG 0.712448 1_2:-64585824 None:intergenic
ATATCTATAGAGGAACACCA+AGG 0.796890 1_2:+64586386 Msa0084880:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTTTATTAACTTGTTTA+AGG + chr1_2:64588602-64588621 Msa0084880:three_prime_UTR 10.0%
!!! TTTTTCCAAATAACAAAATA+TGG - chr1_2:64588162-64588181 None:intergenic 15.0%
!!! ATTTTTTTTATCATTGTCTT+TGG + chr1_2:64588693-64588712 Msa0084880:three_prime_UTR 15.0%
!! TAATTTAATTCTTGCTTAAC+AGG - chr1_2:64587297-64587316 None:intergenic 20.0%
!! TAAGTTTATACCTACTTAAT+TGG + chr1_2:64587499-64587518 Msa0084880:intron 20.0%
!! ATAATTGAGAAGATTAATGT+TGG - chr1_2:64587795-64587814 None:intergenic 20.0%
!!! AAATCAGATGGATTATTATT+TGG - chr1_2:64587908-64587927 None:intergenic 20.0%
!! CAAATAACAAAATATGGTTA+TGG - chr1_2:64588156-64588175 None:intergenic 20.0%
!!! CATAACCATATTTTGTTATT+TGG + chr1_2:64588154-64588173 Msa0084880:CDS 20.0%
!! AATAGAAGAATTATGCAATA+TGG - chr1_2:64588480-64588499 None:intergenic 20.0%
!! TATTGCATAATTCTTCTATT+AGG + chr1_2:64588480-64588499 Msa0084880:three_prime_UTR 20.0%
!!! TTTTTTTTTGGAACAAAATG+AGG - chr1_2:64588526-64588545 None:intergenic 20.0%
!! ATGAATGAATTTCTATATCA+AGG + chr1_2:64588668-64588687 Msa0084880:three_prime_UTR 20.0%
!!! TTTAGCTTTTTTTAACATTG+TGG + chr1_2:64588743-64588762 Msa0084880:three_prime_UTR 20.0%
! TAAAAAGATGTTTGTGGTTA+TGG + chr1_2:64585865-64585884 Msa0084880:exon 25.0%
!! TTTGAAGAAGATTGTTTCTT+TGG + chr1_2:64586163-64586182 Msa0084880:CDS 25.0%
! TTAGCAATAATTGTTTGAAC+AGG - chr1_2:64586334-64586353 None:intergenic 25.0%
! ATTATTGCTAAAGATATGCA+TGG + chr1_2:64586343-64586362 Msa0084880:CDS 25.0%
! AAGTTTAGACATATCTATAG+AGG + chr1_2:64586376-64586395 Msa0084880:CDS 25.0%
! TTTCTAATTCTTCTAGTACT+TGG + chr1_2:64586563-64586582 Msa0084880:CDS 25.0%
! ATAATATTGTTACCTGAAGA+AGG - chr1_2:64586951-64586970 None:intergenic 25.0%
! AACTTAATAACTTTCTCTCA+AGG - chr1_2:64587485-64587504 None:intergenic 25.0%
!!! TTCACATTTTGAAGTAAATC+TGG - chr1_2:64587614-64587633 None:intergenic 25.0%
! TGATTTATGAGGTGAAAATA+TGG - chr1_2:64587737-64587756 None:intergenic 25.0%
! TGATGCAAAAGAACTATTAT+TGG - chr1_2:64587852-64587871 None:intergenic 25.0%
!! TTGTTATTGATACTCTTGTA+GGG - chr1_2:64588031-64588050 None:intergenic 25.0%
!! ATTGTTATTGATACTCTTGT+AGG - chr1_2:64588032-64588051 None:intergenic 25.0%
!! AAATTCTGGTCAAAGTTTTA+AGG + chr1_2:64588099-64588118 Msa0084880:CDS 25.0%
! AAAATATGGTTATGGTTATG+GGG - chr1_2:64588148-64588167 None:intergenic 25.0%
! CAAAATATGGTTATGGTTAT+GGG - chr1_2:64588149-64588168 None:intergenic 25.0%
! ACAAAATATGGTTATGGTTA+TGG - chr1_2:64588150-64588169 None:intergenic 25.0%
! AGTTCAAATATTTCAAAGAG+TGG + chr1_2:64588202-64588221 Msa0084880:CDS 25.0%
!!! AAATGATTTTCAAGACATCA+AGG + chr1_2:64588392-64588411 Msa0084880:three_prime_UTR 25.0%
! TTAAGAATAATTAGCTAGCT+AGG - chr1_2:64588420-64588439 None:intergenic 25.0%
! AAAAACAAATCATACAGCAT+AGG - chr1_2:64588450-64588469 None:intergenic 25.0%
!! TTTTAAGTAGAGATGAAGAC+AGG - chr1_2:64585729-64585748 None:intergenic 30.0%
AAGAGAAAAGAAAAGAAGAG+TGG - chr1_2:64585774-64585793 None:intergenic 30.0%
!!! CATCTTTTTAGAACGAAGTA+GGG - chr1_2:64585855-64585874 None:intergenic 30.0%
!!! ACATCTTTTTAGAACGAAGT+AGG - chr1_2:64585856-64585875 None:intergenic 30.0%
!! TCGTTCTAAAAAGATGTTTG+TGG + chr1_2:64585859-64585878 Msa0084880:exon 30.0%
AATCTCTGCTATGAAATACA+TGG + chr1_2:64586072-64586091 Msa0084880:CDS 30.0%
! TAACTCGTTTTCTCTTAAAG+GGG - chr1_2:64586140-64586159 None:intergenic 30.0%
! CTAACTCGTTTTCTCTTAAA+GGG - chr1_2:64586141-64586160 None:intergenic 30.0%
! TCTAACTCGTTTTCTCTTAA+AGG - chr1_2:64586142-64586161 None:intergenic 30.0%
! GATTGTTTCTTTGGAAACAA+TGG + chr1_2:64586172-64586191 Msa0084880:CDS 30.0%
GGAAATGACAACAGAAAAAA+TGG + chr1_2:64586637-64586656 Msa0084880:CDS 30.0%
AATGACAACAGAAAAAATGG+TGG + chr1_2:64586640-64586659 Msa0084880:CDS 30.0%
! AAATGGTGGTAATGATTTGA+TGG + chr1_2:64586654-64586673 Msa0084880:CDS 30.0%
! AATGGTGGTAATGATTTGAT+GGG + chr1_2:64586655-64586674 Msa0084880:CDS 30.0%
TAATGATTTGATGGGAAAAG+TGG + chr1_2:64586663-64586682 Msa0084880:CDS 30.0%
AATGATTTGATGGGAAAAGT+GGG + chr1_2:64586664-64586683 Msa0084880:CDS 30.0%
! TGTTAATGGAAGATCTTTTG+AGG + chr1_2:64586720-64586739 Msa0084880:CDS 30.0%
! TACTCCTGAATTTTGTGTTA+AGG + chr1_2:64586765-64586784 Msa0084880:CDS 30.0%
GAAACCTTAACACAAAATTC+AGG - chr1_2:64586772-64586791 None:intergenic 30.0%
AATCTGCAATGCAAATTCAA+TGG + chr1_2:64586800-64586819 Msa0084880:CDS 30.0%
!! TTCTAGGATTAGTTGGTTTA+TGG + chr1_2:64586867-64586886 Msa0084880:CDS 30.0%
!! TCTAGGATTAGTTGGTTTAT+GGG + chr1_2:64586868-64586887 Msa0084880:CDS 30.0%
AATTCAGTGCATCAATAAGA+TGG + chr1_2:64587130-64587149 Msa0084880:intron 30.0%
TTACTTCAAGCCAATTAAGT+AGG - chr1_2:64587512-64587531 None:intergenic 30.0%
! TTTATGATGTCATGTTTTCC+AGG + chr1_2:64587574-64587593 Msa0084880:intron 30.0%
AGACTAATTCAACTAACCAA+GGG - chr1_2:64587646-64587665 None:intergenic 30.0%
AGATTAATGTTGGAGATTGA+TGG - chr1_2:64587785-64587804 None:intergenic 30.0%
TTGAAGGGAATTAAATCAGA+TGG - chr1_2:64587920-64587939 None:intergenic 30.0%
ATCATCAACAACCTATAACT+AGG + chr1_2:64587993-64588012 Msa0084880:CDS 30.0%
AAATGTTACTGCATTGTGTA+TGG + chr1_2:64588354-64588373 Msa0084880:CDS 30.0%
TAAACCCTTTGATGAACTAA+AGG + chr1_2:64588574-64588593 Msa0084880:three_prime_UTR 30.0%
AAACCCTTTGATGAACTAAA+GGG + chr1_2:64588575-64588594 Msa0084880:three_prime_UTR 30.0%
!! TTAACTTGTTTAAGGCAATG+AGG + chr1_2:64588610-64588629 Msa0084880:three_prime_UTR 30.0%
!! TAACTTGTTTAAGGCAATGA+GGG + chr1_2:64588611-64588630 Msa0084880:three_prime_UTR 30.0%
TAGTTACATAATTCCACAAG+TGG - chr1_2:64588649-64588668 None:intergenic 30.0%
TTTAACATTGTGGAATGTGA+AGG + chr1_2:64588753-64588772 Msa0084880:three_prime_UTR 30.0%
TTAACATTGTGGAATGTGAA+GGG + chr1_2:64588754-64588773 Msa0084880:three_prime_UTR 30.0%
TTGTTGTGAAGAGAAAGTGA+AGG - chr1_2:64585827-64585846 None:intergenic 35.0%
!! CTTTTCAGAGGTTTTTTCCA+TGG - chr1_2:64585894-64585913 None:intergenic 35.0%
! TGAATCTAAGCACTTTTCAG+AGG - chr1_2:64585906-64585925 None:intergenic 35.0%
AGTGCTTAGATTCACAACTA+TGG + chr1_2:64585912-64585931 Msa0084880:CDS 35.0%
! TAGAAGACTTTTGTGTTGAG+TGG - chr1_2:64585971-64585990 None:intergenic 35.0%
AAAGTCTTCTACTTTCCACA+AGG + chr1_2:64585980-64585999 Msa0084880:CDS 35.0%
TCATAGCAGAGATTCTACAA+GGG - chr1_2:64586066-64586085 None:intergenic 35.0%
TTCATAGCAGAGATTCTACA+AGG - chr1_2:64586067-64586086 None:intergenic 35.0%
TTTGGAAACAATGGCTTAGA+GGG + chr1_2:64586181-64586200 Msa0084880:CDS 35.0%
AATGTCTTTGCAAAAGAAGC+TGG - chr1_2:64586217-64586236 None:intergenic 35.0%
ACCATTGTTTGCATCAGATT+GGG - chr1_2:64586242-64586261 None:intergenic 35.0%
!! TGAGACTATTTTTCCTAGGT+TGG + chr1_2:64586291-64586310 Msa0084880:CDS 35.0%
GCAATAATTGTTTGAACAGG+TGG - chr1_2:64586331-64586350 None:intergenic 35.0%
ATATCTATAGAGGAACACCA+AGG + chr1_2:64586386-64586405 Msa0084880:CDS 35.0%
!! GTGTTTTTGAGAGCAGAAAA+TGG + chr1_2:64586478-64586497 Msa0084880:CDS 35.0%
GAAAATGGAGATCTTTGTGT+TGG + chr1_2:64586493-64586512 Msa0084880:CDS 35.0%
! GAGATCTTTGTGTTGGTATA+AGG + chr1_2:64586500-64586519 Msa0084880:CDS 35.0%
ATGCAAATTCAATGGTGTTC+TGG + chr1_2:64586808-64586827 Msa0084880:CDS 35.0%
!!! CAAAATGCCTTTTGAAACTG+AGG + chr1_2:64586840-64586859 Msa0084880:CDS 35.0%
GAAGAATCCTCAGTTTCAAA+AGG - chr1_2:64586850-64586869 None:intergenic 35.0%
TTGAAACTGAGGATTCTTCT+AGG + chr1_2:64586851-64586870 Msa0084880:CDS 35.0%
!! AGGATTCTTCTAGGATTAGT+TGG + chr1_2:64586860-64586879 Msa0084880:CDS 35.0%
ATGTTCAAGATCCAATTCGT+TGG + chr1_2:64586908-64586927 Msa0084880:CDS 35.0%
GACTCAGAATCAATCAAAGA+TGG - chr1_2:64587004-64587023 None:intergenic 35.0%
CTATGGTAGATAGTACTAGT+AGG - chr1_2:64587436-64587455 None:intergenic 35.0%
ATTCTAGCAAGTAGCAACTA+TGG - chr1_2:64587453-64587472 None:intergenic 35.0%
! AGTTGCTACTTGCTAGAATT+TGG + chr1_2:64587454-64587473 Msa0084880:intron 35.0%
ATGTGAAATGTGTAAACCCT+TGG + chr1_2:64587627-64587646 Msa0084880:CDS 35.0%
GAGACTAATTCAACTAACCA+AGG - chr1_2:64587647-64587666 None:intergenic 35.0%
AATGGTGAGAGATTGAAGTT+TGG - chr1_2:64587677-64587696 None:intergenic 35.0%
ACAAACCTTGGTTTCTTTCT+TGG - chr1_2:64587707-64587726 None:intergenic 35.0%
TATGGATCTTGAACAAACCT+TGG - chr1_2:64587719-64587738 None:intergenic 35.0%
! GCATTGGAAGTTGATTTATG+AGG - chr1_2:64587748-64587767 None:intergenic 35.0%
!! ATTGATGGTGATGATGATGA+TGG - chr1_2:64587770-64587789 None:intergenic 35.0%
! TTCTTTTGCATCAAGCATAC+AGG + chr1_2:64587859-64587878 Msa0084880:CDS 35.0%
! TCTTTTGCATCAAGCATACA+GGG + chr1_2:64587860-64587879 Msa0084880:CDS 35.0%
!! TGTTGTAGTAGCTTGTTGAA+GGG - chr1_2:64587935-64587954 None:intergenic 35.0%
!! TTGTTGTAGTAGCTTGTTGA+AGG - chr1_2:64587936-64587955 None:intergenic 35.0%
CTACAACAAGACATGTTTCT+TGG + chr1_2:64587947-64587966 Msa0084880:CDS 35.0%
! TTGGTAACTTGTCATTGTCA+AGG + chr1_2:64587966-64587985 Msa0084880:CDS 35.0%
!! TTGTCTTGTTTGTTGAGTGT+GGG + chr1_2:64588076-64588095 Msa0084880:CDS 35.0%
TGTTGTTGATCCTTATACCA+AGG - chr1_2:64588310-64588329 None:intergenic 35.0%
! TTTTCTGTTCCAACAAGATC+AGG - chr1_2:64588337-64588356 None:intergenic 35.0%
AACCCTTTGATGAACTAAAG+GGG + chr1_2:64588576-64588595 Msa0084880:three_prime_UTR 35.0%
! TACCCCTTTAGTTCATCAAA+GGG - chr1_2:64588581-64588600 None:intergenic 35.0%
! TTACCCCTTTAGTTCATCAA+AGG - chr1_2:64588582-64588601 None:intergenic 35.0%
GTTTGTGGTTATGGATTCCA+TGG + chr1_2:64585874-64585893 Msa0084880:CDS 40.0%
! AAGACTTTTGTGTTGAGTGG+TGG - chr1_2:64585968-64585987 None:intergenic 40.0%
CGTAGACTTCATCAGTTTCA+GGG - chr1_2:64586102-64586121 None:intergenic 40.0%
ACGTAGACTTCATCAGTTTC+AGG - chr1_2:64586103-64586122 None:intergenic 40.0%
! CTTTGGAAACAATGGCTTAG+AGG + chr1_2:64586180-64586199 Msa0084880:CDS 40.0%
ACCCAATCTGATGCAAACAA+TGG + chr1_2:64586238-64586257 Msa0084880:CDS 40.0%
CACCATTGTTTGCATCAGAT+TGG - chr1_2:64586243-64586262 None:intergenic 40.0%
CAATCTGATGCAAACAATGG+TGG + chr1_2:64586241-64586260 Msa0084880:CDS 40.0%
ATAGTCTCAGCACAGTATCT+AGG - chr1_2:64586280-64586299 None:intergenic 40.0%
!! GTGCTGAGACTATTTTTCCT+AGG + chr1_2:64586287-64586306 Msa0084880:CDS 40.0%
TCTGCTGAATAATCCAACCT+AGG - chr1_2:64586307-64586326 None:intergenic 40.0%
AAGATATGCATGGACAGTGT+TGG + chr1_2:64586353-64586372 Msa0084880:CDS 40.0%
GTTGTCAAAAGATGTCTCCT+TGG - chr1_2:64586406-64586425 None:intergenic 40.0%
! AGGAGACATCTTTTGACAAC+TGG + chr1_2:64586406-64586425 Msa0084880:CDS 40.0%
! GACATCTTTTGACAACTGGT+TGG + chr1_2:64586410-64586429 Msa0084880:CDS 40.0%
!! AATCACAAGAAGCTTGTTGC+TGG + chr1_2:64586445-64586464 Msa0084880:CDS 40.0%
GGTATAAGGAGAGCAAAGAA+AGG + chr1_2:64586514-64586533 Msa0084880:CDS 40.0%
ATAAGGAGAGCAAAGAAAGG+TGG + chr1_2:64586517-64586536 Msa0084880:CDS 40.0%
AGAGCAAAGAAAGGTGGAAT+TGG + chr1_2:64586523-64586542 Msa0084880:CDS 40.0%
AACCGTGTATCACCTTTGTT+TGG + chr1_2:64586586-64586605 Msa0084880:CDS 40.0%
CACCAAACAAAGGTGATACA+CGG - chr1_2:64586591-64586610 None:intergenic 40.0%
! GCTGTAAACTGTGCTGTTAA+TGG + chr1_2:64586706-64586725 Msa0084880:CDS 40.0%
!! AATTCAGGAGTACTAGCTCT+TGG - chr1_2:64586757-64586776 None:intergenic 40.0%
! TCATGTTTTCCAGGTAGTGT+GGG + chr1_2:64587583-64587602 Msa0084880:intron 40.0%
! TTCTTTCTTGGAGGTGTGAA+TGG - chr1_2:64587695-64587714 None:intergenic 40.0%
AACCTTGGTTTCTTTCTTGG+AGG - chr1_2:64587704-64587723 None:intergenic 40.0%
ATACTCTTGTAGGGTCCATA+AGG - chr1_2:64588022-64588041 None:intergenic 40.0%
!! CTTGTCTTGTTTGTTGAGTG+TGG + chr1_2:64588075-64588094 Msa0084880:CDS 40.0%
TTGTTGAGTGTGGGAAATTC+TGG + chr1_2:64588085-64588104 Msa0084880:CDS 40.0%
! GGGTGCTTTTGCTTCAATAT+TGG - chr1_2:64588128-64588147 None:intergenic 40.0%
!! AAGAGTGGTTCTGTTTCTGA+AGG + chr1_2:64588217-64588236 Msa0084880:CDS 40.0%
CCATCAGAAGAATTCTCAAC+TGG - chr1_2:64588280-64588299 None:intergenic 40.0%
CCAGTTGAGAATTCTTCTGA+TGG + chr1_2:64588277-64588296 Msa0084880:CDS 40.0%
GTTGAGAATTCTTCTGATGG+AGG + chr1_2:64588280-64588299 Msa0084880:CDS 40.0%
GAAAATTCCTCTTCCACTTG+TGG + chr1_2:64588633-64588652 Msa0084880:three_prime_UTR 40.0%
CATAATTCCACAAGTGGAAG+AGG - chr1_2:64588643-64588662 None:intergenic 40.0%
GTGTGGTTGGATGAGGAAAA+AGG - chr1_2:64585805-64585824 None:intergenic 45.0%
! AGAGAAAGTGAAGGTGTGGT+TGG - chr1_2:64585818-64585837 None:intergenic 45.0%
GTGAAGAGAAAGTGAAGGTG+TGG - chr1_2:64585822-64585841 None:intergenic 45.0%
ATTCTACAAGGGATGAGAGG+TGG - chr1_2:64586055-64586074 None:intergenic 45.0%
GAGATTCTACAAGGGATGAG+AGG - chr1_2:64586058-64586077 None:intergenic 45.0%
TCTGATGCAAACAATGGTGG+TGG + chr1_2:64586244-64586263 Msa0084880:CDS 45.0%
GCAAAGAAAGGTGGAATTGG+TGG + chr1_2:64586526-64586545 Msa0084880:CDS 45.0%
AAGAAAGGTGGAATTGGTGG+TGG + chr1_2:64586529-64586548 Msa0084880:CDS 45.0%
! CGTGTATCACCTTTGTTTGG+TGG + chr1_2:64586589-64586608 Msa0084880:CDS 45.0%
! GTATCACCTTTGTTTGGTGG+TGG + chr1_2:64586592-64586611 Msa0084880:CDS 45.0%
! GTCATGTTTTCCAGGTAGTG+TGG + chr1_2:64587582-64587601 Msa0084880:intron 45.0%
CACCTCCAAGAAAGAAACCA+AGG + chr1_2:64587699-64587718 Msa0084880:CDS 45.0%
!! GGTGATGATGATGATGGCAT+TGG - chr1_2:64587764-64587783 None:intergenic 45.0%
CCATAAGGAGGCCTAGTTAT+AGG - chr1_2:64588007-64588026 None:intergenic 45.0%
CCTATAACTAGGCCTCCTTA+TGG + chr1_2:64588004-64588023 Msa0084880:CDS 45.0%
CAACACAAGCCTGATCTTGT+TGG + chr1_2:64588325-64588344 Msa0084880:CDS 45.0%
!!! ACATAATATTAATTTTTTTT+TGG - chr1_2:64588538-64588557 None:intergenic 5.0%
! AGTGAAGGTGTGGTTGGATG+AGG - chr1_2:64585812-64585831 None:intergenic 50.0%
GAGTGGTGGCATTTGAACCA+TGG - chr1_2:64585954-64585973 None:intergenic 50.0%
GCATGTTCTGCATGACCTTG+TGG - chr1_2:64585998-64586017 None:intergenic 50.0%
AAGGGATGAGAGGTGGAACA+CGG - chr1_2:64586048-64586067 None:intergenic 50.0%
!! TCACCTTTGTTTGGTGGTGG+TGG + chr1_2:64586595-64586614 Msa0084880:CDS 50.0%
!! GGTGGTTCTGGTTTCTTGTG+TGG + chr1_2:64586616-64586635 Msa0084880:CDS 50.0%
GGAGAATCAGGCCAACGAAT+TGG - chr1_2:64586922-64586941 None:intergenic 50.0%
! TTCGTTGGCCTGATTCTCCT+TGG + chr1_2:64586923-64586942 Msa0084880:CDS 50.0%
TGGTCCAAATTGAGCATGCC+TGG - chr1_2:64587888-64587907 None:intergenic 50.0%
CTCTTGTAGGGTCCATAAGG+AGG - chr1_2:64588019-64588038 None:intergenic 50.0%
TGGAGGCTCTCCTTGGTATA+AGG + chr1_2:64588297-64588316 Msa0084880:CDS 50.0%
CAACTATGGCACGCATGTGC+TGG + chr1_2:64585926-64585945 Msa0084880:CDS 55.0%
! GAGAGGTGGAACACGGGTTT+TGG - chr1_2:64586041-64586060 None:intergenic 55.0%
AGGGATGAGAGGTGGAACAC+GGG - chr1_2:64586047-64586066 None:intergenic 55.0%
CCACCACCACCACCAAACAA+AGG - chr1_2:64586601-64586620 None:intergenic 55.0%
!! CCTTTGTTTGGTGGTGGTGG+TGG + chr1_2:64586598-64586617 Msa0084880:CDS 55.0%
!! TTTGGTGGTGGTGGTGGTTC+TGG + chr1_2:64586604-64586623 Msa0084880:CDS 55.0%
!! AGAAGGCGCCAAGGAGAATC+AGG - chr1_2:64586934-64586953 None:intergenic 55.0%
TTCTCCTTGGCGCCTTCTTC+AGG + chr1_2:64586936-64586955 Msa0084880:CDS 55.0%
!! GTTACCTGAAGAAGGCGCCA+AGG - chr1_2:64586943-64586962 None:intergenic 55.0%
CTGGTTCATCCCACACTACC+TGG - chr1_2:64587595-64587614 None:intergenic 55.0%
CATCAAGCATACAGGGAGCC+AGG + chr1_2:64587867-64587886 Msa0084880:CDS 55.0%
GGAGCCAGGCATGCTCAATT+TGG + chr1_2:64587881-64587900 Msa0084880:CDS 55.0%
CTTCTGATGGAGGCTCTCCT+TGG + chr1_2:64588290-64588309 Msa0084880:CDS 55.0%
! GCACGCATGTGCTGGTGCCA+TGG + chr1_2:64585934-64585953 Msa0084880:CDS 65.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 64585688 64588786 64585688 ID=Msa0084880;Name=Msa0084880
chr1_2 mRNA 64585688 64588786 64585688 ID=Msa0084880-mRNA-1;Parent=Msa0084880;Name=Msa0084880-mRNA-1;_AED=0.00;_eAED=0.00;_QI=184|1|1|1|1|1|2|402|624
chr1_2 exon 64585688 64586957 64585688 ID=Msa0084880-mRNA-1:exon:21796;Parent=Msa0084880-mRNA-1
chr1_2 exon 64587596 64588786 64587596 ID=Msa0084880-mRNA-1:exon:21797;Parent=Msa0084880-mRNA-1
chr1_2 five_prime_UTR 64585688 64585871 64585688 ID=Msa0084880-mRNA-1:five_prime_utr;Parent=Msa0084880-mRNA-1
chr1_2 CDS 64585872 64586957 64585872 ID=Msa0084880-mRNA-1:cds;Parent=Msa0084880-mRNA-1
chr1_2 CDS 64587596 64588384 64587596 ID=Msa0084880-mRNA-1:cds;Parent=Msa0084880-mRNA-1
chr1_2 three_prime_UTR 64588385 64588786 64588385 ID=Msa0084880-mRNA-1:three_prime_utr;Parent=Msa0084880-mRNA-1
Gene Sequence

>Msa0084880

ATGTTTGTGGTTATGGATTCCATGGAAAAAACCTCTGAAAAGTGCTTAGATTCACAACTATGGCACGCATGTGCTGGTGCCATGGTTCAAATGCCACCACTCAACACAAAAGTCTTCTACTTTCCACAAGGTCATGCAGAACATGCTCATAACAAAGTAGATTTTTCCAAAACCCGTGTTCCACCTCTCATCCCTTGTAGAATCTCTGCTATGAAATACATGGCAGACCCTGAAACTGATGAAGTCTACGTCAAAATGAAACTAACCCCTTTAAGAGAAAACGAGTTAGATTTTGAAGAAGATTGTTTCTTTGGAAACAATGGCTTAGAGGGTCAAGAAAAGCCAGCTTCTTTTGCAAAGACATTAACCCAATCTGATGCAAACAATGGTGGTGGTTTTTCTGTTCCTAGATACTGTGCTGAGACTATTTTTCCTAGGTTGGATTATTCAGCAGAACCACCTGTTCAAACAATTATTGCTAAAGATATGCATGGACAGTGTTGGAAGTTTAGACATATCTATAGAGGAACACCAAGGAGACATCTTTTGACAACTGGTTGGAGTAATTTTGTGAATCACAAGAAGCTTGTTGCTGGTGATTCAATTGTGTTTTTGAGAGCAGAAAATGGAGATCTTTGTGTTGGTATAAGGAGAGCAAAGAAAGGTGGAATTGGTGGTGGAACTGATCAGTTTTCTAATTCTTCTAGTACTTGGAACCGTGTATCACCTTTGTTTGGTGGTGGTGGTGGTTCTGGTTTCTTGTGTGGAAATGACAACAGAAAAAATGGTGGTAATGATTTGATGGGAAAAGTGGGAGCTGAATCTGTTGTTGAAGCTGTAAACTGTGCTGTTAATGGAAGATCTTTTGAGGTTGTGTATTATCCAAGAGCTAGTACTCCTGAATTTTGTGTTAAGGTTTCTTCTGTTAAATCTGCAATGCAAATTCAATGGTGTTCTGGTATGAGATTCAAAATGCCTTTTGAAACTGAGGATTCTTCTAGGATTAGTTGGTTTATGGGAACAATTTCTTCTGTTCATGTTCAAGATCCAATTCGTTGGCCTGATTCTCCTTGGCGCCTTCTTCAGGTAGTGTGGGATGAACCAGATTTACTTCAAAATGTGAAATGTGTAAACCCTTGGTTAGTTGAATTAGTCTCAAACATGCCAAACTTCAATCTCTCACCATTCACACCTCCAAGAAAGAAACCAAGGTTTGTTCAAGATCCATATTTTCACCTCATAAATCAACTTCCAATGCCATCATCATCATCACCATCAATCTCCAACATTAATCTTCTCAATTATACAAAATCTTCTCTCTGTAGCATTCAAGACACTACCAATAATAGTTCTTTTGCATCAAGCATACAGGGAGCCAGGCATGCTCAATTTGGACCAAATAATAATCCATCTGATTTAATTCCCTTCAACAAGCTACTACAACAAGACATGTTTCTTGGTAACTTGTCATTGTCAAGGTTTAATCATCAACAACCTATAACTAGGCCTCCTTATGGACCCTACAAGAGTATCAATAACAATAACAACACTAAGAACAATGTTGACTTGTCTTGTTTGTTGAGTGTGGGAAATTCTGGTCAAAGTTTTAAGGAATCCAATATTGAAGCAAAAGCACCCCATAACCATAACCATATTTTGTTATTTGGAAAAATCATTCACACTGAACAAAACAGTTCAAATATTTCAAAGAGTGGTTCTGTTTCTGAAGGAACTTCTTTGAAAACATCAAATGCTTCTTCTTCTGATCCAGTTGAGAATTCTTCTGATGGAGGCTCTCCTTGGTATAAGGATCAACAACACAAGCCTGATCTTGTTGGAACAGAAAATGTTACTGCATTGTGTATGGCTTCATAA
Protein sequence

>Msa0084880

MFVVMDSMEKTSEKCLDSQLWHACAGAMVQMPPLNTKVFYFPQGHAEHAHNKVDFSKTRVPPLIPCRISAMKYMADPETDEVYVKMKLTPLRENELDFEEDCFFGNNGLEGQEKPASFAKTLTQSDANNGGGFSVPRYCAETIFPRLDYSAEPPVQTIIAKDMHGQCWKFRHIYRGTPRRHLLTTGWSNFVNHKKLVAGDSIVFLRAENGDLCVGIRRAKKGGIGGGTDQFSNSSSTWNRVSPLFGGGGGSGFLCGNDNRKNGGNDLMGKVGAESVVEAVNCAVNGRSFEVVYYPRASTPEFCVKVSSVKSAMQIQWCSGMRFKMPFETEDSSRISWFMGTISSVHVQDPIRWPDSPWRLLQVVWDEPDLLQNVKCVNPWLVELVSNMPNFNLSPFTPPRKKPRFVQDPYFHLINQLPMPSSSSPSISNINLLNYTKSSLCSIQDTTNNSSFASSIQGARHAQFGPNNNPSDLIPFNKLLQQDMFLGNLSLSRFNHQQPITRPPYGPYKSINNNNNTKNNVDLSCLLSVGNSGQSFKESNIEAKAPHNHNHILLFGKIIHTEQNSSNISKSGSVSEGTSLKTSNASSSDPVENSSDGGSPWYKDQQHKPDLVGTENVTALCMAS*