AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0087560

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0087560 MtrunA17_Chr1g0204501 96.753 154 5 0 74 227 45 198 4.35e-107 306
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0087560 AT5G19910.1 68.790 157 32 4 74 227 51 193 1.99e-57 181
Msa0087560 AT5G19910.2 57.754 187 32 5 74 227 51 223 9.33e-51 165

Find 128 sgRNAs with CRISPR-Local

Find 239 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTAATAGAAGTATGATTT+TGG 0.136395 1_2:+68530288 Msa0087560:exon
GATATCACTATTTACCTTAT+TGG 0.153158 1_2:-68531681 None:intergenic
TGATCGACCTTTGTTATAAT+TGG 0.166073 1_2:+68530011 Msa0087560:five_prime_UTR
GTAGTGCATGCACAGGTGGC+TGG 0.230176 1_2:-68532239 None:intergenic
GTTTCGTCCATTTGTTTCTA+AGG 0.240006 1_2:+68529584 Msa0087560:five_prime_UTR
GGAGTTGAAGTAATAATAAT+AGG 0.256428 1_2:+68532449 Msa0087560:three_prime_UTR
GGATCCTTGTATATATTCTT+TGG 0.257913 1_2:-68530366 None:intergenic
TCAAGCCACCCTGCATAAAT+TGG 0.272984 1_2:+68530155 Msa0087560:five_prime_UTR
GAACCTAGTGCTGCGCTTCC+TGG 0.281659 1_2:+68532198 Msa0087560:CDS
CAGTTTGAAAATTTAATATA+TGG 0.284142 1_2:+68530046 Msa0087560:five_prime_UTR
AGAAAGTGAGTGCTTTATAT+TGG 0.289671 1_2:+68532396 Msa0087560:exon
ACTTAAAGTATCTTCAATAT+TGG 0.293258 1_2:+68531314 Msa0087560:CDS
TTTGTTATAATTGGCATATC+TGG 0.303686 1_2:+68530020 Msa0087560:five_prime_UTR
ATCTATTGTGCAGCACTTGT+TGG 0.309094 1_2:-68529754 None:intergenic
TATTTAAATGGTACAAGCTT+TGG 0.323173 1_2:+68532706 Msa0087560:three_prime_UTR
CATGCTTTGTCATTGCTGCT+TGG 0.324180 1_2:+68531176 Msa0087560:CDS
TGTATCAAAGCTCCCAATCT+TGG 0.330312 1_2:-68529635 None:intergenic
ATGATAAATTTAATATATTC+AGG 0.332776 1_2:-68531343 None:intergenic
GGAGTATGTCTGTATTTAAA+TGG 0.347406 1_2:+68532694 Msa0087560:three_prime_UTR
AACCGTTGTCGCCCATCATC+TGG 0.352900 1_2:-68530387 None:intergenic
GTTTCTTATTCTCCTCGGAT+TGG 0.357717 1_2:+68529935 Msa0087560:five_prime_UTR
ATTAAAGGTGAGAGCTAAAC+AGG 0.361539 1_2:+68529692 Msa0087560:five_prime_UTR
AAATTAAAATCTGTAGTATA+CGG 0.364774 1_2:-68530239 None:intergenic
ATTTCATACTCTCCCAAGAT+TGG 0.365797 1_2:+68529622 Msa0087560:five_prime_UTR
CTAGGTTCTGGAAGTGGTCT+TGG 0.382881 1_2:-68532183 None:intergenic
TAGTGCATGCACAGGTGGCT+GGG 0.384524 1_2:-68532238 None:intergenic
TTTCTTATTCTCCTCGGATT+GGG 0.385548 1_2:+68529936 Msa0087560:five_prime_UTR
AATGGCACATCCTACCAATA+AGG 0.389767 1_2:+68531667 Msa0087560:CDS
GGAAGCGCAGCACTAGGTTC+TGG 0.393441 1_2:-68532195 None:intergenic
TGTGCTACCCAACTTAAAAT+AGG 0.393795 1_2:-68532523 None:intergenic
AGAAGGGACATATGCCAATT+GGG 0.399848 1_2:-68529803 None:intergenic
CAGAAGGGACATATGCCAAT+TGG 0.400766 1_2:-68529804 None:intergenic
TTCTAGTTTCTTATTCTCCT+CGG 0.403178 1_2:+68529930 Msa0087560:five_prime_UTR
TCTACTTCTGGAAGAACTAT+AGG 0.406034 1_2:+68532136 Msa0087560:CDS
GAAATACTTCAGCTGATAGA+AGG 0.409099 1_2:+68532370 Msa0087560:CDS
TTATCGCTCCACTCCCCAAT+TGG 0.410613 1_2:+68529789 Msa0087560:five_prime_UTR
TTTGAGGACGAAGCATTCAT+TGG 0.411048 1_2:+68531289 Msa0087560:CDS
GCGCTTAAGTACAGCTCAAC+AGG 0.415512 1_2:-68530322 None:intergenic
GAATGGAACACAAATACTAT+TGG 0.422824 1_2:-68530184 None:intergenic
GAGACGGATAAAACAGTTAG+AGG 0.430861 1_2:-68529835 None:intergenic
AAATTTGATAAAACAGAGAT+TGG 0.431232 1_2:+68529657 Msa0087560:five_prime_UTR
GGCACACACTTCACGCTGAA+TGG 0.436638 1_2:-68530201 None:intergenic
TGGTTATAACCAATTTATGC+AGG 0.437509 1_2:-68530164 None:intergenic
GTATCAAAGCTCCCAATCTT+GGG 0.440002 1_2:-68529634 None:intergenic
ATGCTTCGTCCTCAAAATAT+CGG 0.443322 1_2:-68531282 None:intergenic
TAGAAACAAATGGACGAAAC+AGG 0.452632 1_2:-68529581 None:intergenic
TAGTGCTGCGCTTCCTGGTC+CGG 0.453206 1_2:+68532203 Msa0087560:CDS
ATAGACAGCAGTTCTACTTC+TGG 0.455964 1_2:+68532124 Msa0087560:CDS
AGTGCATGCACAGGTGGCTG+GGG 0.456575 1_2:-68532237 None:intergenic
GGTTGAGTCGAGGCCGGACC+AGG 0.460454 1_2:-68532216 None:intergenic
TTTCATACTCTCCCAAGATT+GGG 0.461521 1_2:+68529623 Msa0087560:five_prime_UTR
CAGCCGTGGCTTGTCATACA+AGG 0.470099 1_2:-68529501 None:intergenic
GGCTGGGGTTGAGTCGAGGC+CGG 0.471060 1_2:-68532222 None:intergenic
TAAGGAGAGGCACTTTATGC+TGG 0.471146 1_2:+68530079 Msa0087560:five_prime_UTR
GAGTGTAAGCCAACCAGGCC+TGG 0.473699 1_2:+68530111 Msa0087560:five_prime_UTR
TCACTATTTACCTTATTGGT+AGG 0.479734 1_2:-68531677 None:intergenic
AGGGAAGCCTTAGAAACAAA+TGG 0.488857 1_2:-68529591 None:intergenic
TATACGGAAAGCCCTTGAAA+AGG 0.490993 1_2:-68530223 None:intergenic
TGATAAATTTAATATATTCA+GGG 0.495109 1_2:-68531342 None:intergenic
GAAGGATGAACGATGTATCA+AGG 0.498494 1_2:-68529881 None:intergenic
GGAAGAACTATAGGAACAAT+CGG 0.500395 1_2:+68532145 Msa0087560:CDS
TGGGAGAGTATGAAATCTAC+AGG 0.502485 1_2:-68529615 None:intergenic
GAGTTGAAGTAATAATAATA+GGG 0.503829 1_2:+68532450 Msa0087560:three_prime_UTR
TTGAGGACGAAGCATTCATT+GGG 0.512149 1_2:+68531290 Msa0087560:CDS
TCTAACGAGTTTCAACCGTC+TGG 0.512281 1_2:-68532567 None:intergenic
ATACTTTCTTCCATCGCAGA+TGG 0.517896 1_2:+68532427 Msa0087560:three_prime_UTR
GCAACACTTGTAGCAGGTAC+TGG 0.519969 1_2:-68532264 None:intergenic
GAAGAAGCTCAAGAAAATAG+AGG 0.520705 1_2:-68531622 None:intergenic
GAGTGGGGAGTCACAGAGGA+AGG 0.522359 1_2:-68529462 None:intergenic
AAAATGAAATTGATCAAGAC+AGG 0.523673 1_2:-68530264 None:intergenic
ATATACAAGGATCCAGATGA+TGG 0.525951 1_2:+68530375 Msa0087560:CDS
AAGGTAATTGACAGGAAAAG+AGG 0.529830 1_2:-68529558 None:intergenic
AAAAGAGCAGACTGATCAAA+GGG 0.534798 1_2:-68529976 None:intergenic
GGTTATAACCAATTTATGCA+GGG 0.536876 1_2:-68530163 None:intergenic
GTTATGAGTGTAAGCCAACC+AGG 0.539396 1_2:+68530106 Msa0087560:five_prime_UTR
ACAGGAAAAGAGGCTAATGA+TGG 0.543975 1_2:-68529548 None:intergenic
AAGGATGAACGATGTATCAA+GGG 0.547169 1_2:-68529880 None:intergenic
GAGCTGTACTTAAGCGCTGA+TGG 0.548171 1_2:+68530328 Msa0087560:CDS
GGACCAGGAAGCGCAGCACT+AGG 0.549090 1_2:-68532201 None:intergenic
GCAGCACTAGGTTCTGGAAG+TGG 0.553833 1_2:-68532189 None:intergenic
ATACGGAAAGCCCTTGAAAA+GGG 0.555379 1_2:-68530222 None:intergenic
CAAAAGAGCAGACTGATCAA+AGG 0.567861 1_2:-68529977 None:intergenic
ATACCGTATGGCATTGGAGA+AGG 0.568373 1_2:-68532312 None:intergenic
TACTTTCTTCCATCGCAGAT+GGG 0.571187 1_2:+68532428 Msa0087560:three_prime_UTR
GACATATGCCAATTGGGGAG+TGG 0.571738 1_2:-68529797 None:intergenic
GAAAACCATACAGTGGATGT+AGG 0.571854 1_2:-68530451 None:intergenic
GTAAGCCAACCAGGCCTGGT+TGG 0.572231 1_2:+68530115 Msa0087560:five_prime_UTR
AGGTGGCTGGGGTTGAGTCG+AGG 0.574599 1_2:-68532226 None:intergenic
GTTGATCATGACTAATGAGT+GGG 0.575424 1_2:-68529478 None:intergenic
AACCCTTGTATGACAAGCCA+CGG 0.581906 1_2:+68529498 Msa0087560:five_prime_UTR
GAAACAGGAAGGTAATTGAC+AGG 0.583410 1_2:-68529566 None:intergenic
AGAGTATGAAATCTACAGGA+AGG 0.585843 1_2:-68529611 None:intergenic
GTTAGAGGAGGAATGACAGA+AGG 0.586720 1_2:-68529820 None:intergenic
GTCACAGCAACACTTGTAGC+AGG 0.586975 1_2:-68532270 None:intergenic
ACCATACAGTGGATGTAGGT+AGG 0.594759 1_2:-68530447 None:intergenic
ATCCAGATGATGGGCGACAA+CGG 0.595215 1_2:+68530385 Msa0087560:CDS
GCTCCTTCTCCAATGCCATA+CGG 0.595355 1_2:+68532309 Msa0087560:CDS
GCTTGCAGTGAGAGCAGCCG+TGG 0.596439 1_2:-68529515 None:intergenic
ACTTCAACTCCCATCTGCGA+TGG 0.598476 1_2:-68532437 None:intergenic
TTGATGTTACAAATGATGAA+AGG 0.600431 1_2:+68532673 Msa0087560:three_prime_UTR
GGTGGTAGTGCATGCACAGG+TGG 0.607758 1_2:-68532243 None:intergenic
TATACAAGGATCCAGATGAT+GGG 0.614053 1_2:+68530376 Msa0087560:CDS
GAGTATGAAATCTACAGGAA+GGG 0.616379 1_2:-68529610 None:intergenic
AAACAGGAAAGGTGGAATGA+CGG 0.625544 1_2:+68529708 Msa0087560:five_prime_UTR
AGATATGCCAATTATAACAA+AGG 0.628476 1_2:-68530018 None:intergenic
ACGGATAAAACAGTTAGAGG+AGG 0.632952 1_2:-68529832 None:intergenic
TCCATATAGTTAAAGGCGGG+GGG 0.633111 1_2:+68529371 None:intergenic
TGAGAGCTAAACAGGAAAGG+TGG 0.639079 1_2:+68529700 Msa0087560:five_prime_UTR
AACAAATGGACGAAACAGGA+AGG 0.640927 1_2:-68529577 None:intergenic
TAGGCCAAAGAATATATACA+AGG 0.644719 1_2:+68530362 Msa0087560:CDS
GAAGGGACATATGCCAATTG+GGG 0.644738 1_2:-68529802 None:intergenic
TCAAGAAAATAGAGGCAGTG+AGG 0.646000 1_2:-68531614 None:intergenic
TTAGAGGAGGAATGACAGAA+GGG 0.646052 1_2:-68529819 None:intergenic
GGTTGATCATGACTAATGAG+TGG 0.646535 1_2:-68529479 None:intergenic
AGTGGGGAGTCACAGAGGAA+GGG 0.648009 1_2:-68529461 None:intergenic
AGGTGAGAGCTAAACAGGAA+AGG 0.648812 1_2:+68529697 Msa0087560:five_prime_UTR
TATAACCAATTTATGCAGGG+TGG 0.649169 1_2:-68530160 None:intergenic
GGATGTAGGTAGGATTAGCA+AGG 0.649374 1_2:-68530437 None:intergenic
ATTGAAAATACATATCCAGA+CGG 0.649827 1_2:+68532552 Msa0087560:three_prime_UTR
ACTGGTGGTAGTGCATGCAC+AGG 0.653824 1_2:-68532246 None:intergenic
TGCTGTCTAAACCCAATCCG+AGG 0.660940 1_2:-68529947 None:intergenic
AGGGAAAACATTCATCACAA+AGG 0.672318 1_2:-68529442 None:intergenic
AGGATGAACGATGTATCAAG+GGG 0.675967 1_2:-68529879 None:intergenic
TAATGAGTGGGGAGTCACAG+AGG 0.683280 1_2:-68529466 None:intergenic
AGCCGTGGCTTGTCATACAA+GGG 0.688236 1_2:-68529500 None:intergenic
ACACTTGTAGCAGGTACTGG+TGG 0.693490 1_2:-68532261 None:intergenic
TTGATCATGACTAATGAGTG+GGG 0.702989 1_2:-68529477 None:intergenic
TTTGCACTACCGGAGCCAGG+GGG 0.746793 1_2:-68532336 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGATAAATTTAATATATTCA+GGG - chr1_2:68531345-68531364 None:intergenic 10.0%
!! ATGATAAATTTAATATATTC+AGG - chr1_2:68531346-68531365 None:intergenic 10.0%
!!! AATTTCAAATTATCTTTTAA+TGG + chr1_2:68532622-68532641 Msa0087560:three_prime_UTR 10.0%
!! CAGTTTGAAAATTTAATATA+TGG + chr1_2:68530046-68530065 Msa0087560:five_prime_UTR 15.0%
!! AAATTAAAATCTGTAGTATA+CGG - chr1_2:68530242-68530261 None:intergenic 15.0%
!!! ATTAATAGAAGTATGATTTT+GGG + chr1_2:68530289-68530308 Msa0087560:exon 15.0%
!!! TTTATTTAATTTCAAGTACA+TGG + chr1_2:68530673-68530692 Msa0087560:intron 15.0%
!!! GAAATAATTTTACAATTCAT+TGG - chr1_2:68532599-68532618 None:intergenic 15.0%
!! AAATTTGATAAAACAGAGAT+TGG + chr1_2:68529657-68529676 Msa0087560:five_prime_UTR 20.0%
!! CAAAAATAAAAAATCAGAGA+CGG - chr1_2:68529854-68529873 None:intergenic 20.0%
!!! CATTAATAGAAGTATGATTT+TGG + chr1_2:68530288-68530307 Msa0087560:exon 20.0%
!! AATTGTTTAGTTAGAAAACA+AGG + chr1_2:68530562-68530581 Msa0087560:intron 20.0%
!! ATGTTGGAATTATAAGTAAT+CGG + chr1_2:68530603-68530622 Msa0087560:intron 20.0%
!!! ATGTTTTTTTGTCAAAAAAG+GGG - chr1_2:68531154-68531173 None:intergenic 20.0%
!!! CATGTTTTTTTGTCAAAAAA+GGG - chr1_2:68531155-68531174 None:intergenic 20.0%
!!! ACTTAAAGTATCTTCAATAT+TGG + chr1_2:68531314-68531333 Msa0087560:CDS 20.0%
!!! ATGTTTTACAATTCTACTTA+AGG + chr1_2:68531413-68531432 Msa0087560:intron 20.0%
!!! TGTTTTACAATTCTACTTAA+GGG + chr1_2:68531414-68531433 Msa0087560:intron 20.0%
!!! AGAAATCATGATTTTGTAAA+AGG - chr1_2:68531714-68531733 None:intergenic 20.0%
!!! ATTACTTGCATTTTAAGTTT+AGG + chr1_2:68532040-68532059 Msa0087560:intron 20.0%
!! GAGTTGAAGTAATAATAATA+GGG + chr1_2:68532450-68532469 Msa0087560:three_prime_UTR 20.0%
! CATGTTAGAAAAATTGTTAG+TGG + chr1_2:68529406-68529425 Msa0087560:five_prime_UTR 25.0%
! TGGTAAGCCTAAAAAATTAA+AGG + chr1_2:68529677-68529696 Msa0087560:five_prime_UTR 25.0%
! AGATATGCCAATTATAACAA+AGG - chr1_2:68530021-68530040 None:intergenic 25.0%
!! TTTGTTATAATTGGCATATC+TGG + chr1_2:68530020-68530039 Msa0087560:five_prime_UTR 25.0%
! ATATATGGTGCAAAAAGATA+AGG + chr1_2:68530061-68530080 Msa0087560:five_prime_UTR 25.0%
! AAAATGAAATTGATCAAGAC+AGG - chr1_2:68530267-68530286 None:intergenic 25.0%
! ATAAAATGAAAACCATACAG+TGG - chr1_2:68530461-68530480 None:intergenic 25.0%
!!! AGATTATTCTAGAGTAGATT+AGG + chr1_2:68530641-68530660 Msa0087560:intron 25.0%
! AGAAATTACAATGTGTTTCA+AGG - chr1_2:68531113-68531132 None:intergenic 25.0%
! ATTGTAATTTCTCTTAATCC+AGG + chr1_2:68531121-68531140 Msa0087560:intron 25.0%
!!! CCATGTTTTTTTGTCAAAAA+AGG - chr1_2:68531156-68531175 None:intergenic 25.0%
!!! CCTTTTTTGACAAAAAAACA+TGG + chr1_2:68531153-68531172 Msa0087560:CDS 25.0%
! GCACTGAATTATGTTAAATA+GGG + chr1_2:68531436-68531455 Msa0087560:intron 25.0%
!! ACTCTATAAGAATTTTACAC+AGG + chr1_2:68531461-68531480 Msa0087560:intron 25.0%
!! CTCTATAAGAATTTTACACA+GGG + chr1_2:68531462-68531481 Msa0087560:intron 25.0%
! TAGAATAAAAATGACAGAAC+TGG - chr1_2:68531529-68531548 None:intergenic 25.0%
! GATATCACTATTTACCTTAT+TGG - chr1_2:68531684-68531703 None:intergenic 25.0%
! AGTACTATAATATGAAATCC+AGG - chr1_2:68531737-68531756 None:intergenic 25.0%
! AACTTAAAATGCAAGTAATC+TGG - chr1_2:68532039-68532058 None:intergenic 25.0%
! ACCAAAAATTTATCTGTTGT+AGG + chr1_2:68532092-68532111 Msa0087560:intron 25.0%
!! TCCTACAACAGATAAATTTT+TGG - chr1_2:68532096-68532115 None:intergenic 25.0%
! GGAGTTGAAGTAATAATAAT+AGG + chr1_2:68532449-68532468 Msa0087560:three_prime_UTR 25.0%
!! TCTGGATATGTATTTTCAAT+TGG - chr1_2:68532552-68532571 None:intergenic 25.0%
! ATTGAAAATACATATCCAGA+CGG + chr1_2:68532552-68532571 Msa0087560:three_prime_UTR 25.0%
! TTGATGTTACAAATGATGAA+AGG + chr1_2:68532673-68532692 Msa0087560:three_prime_UTR 25.0%
! TATTTAAATGGTACAAGCTT+TGG + chr1_2:68532706-68532725 Msa0087560:three_prime_UTR 25.0%
AAAAATTGTTAGTGGTTGTG+AGG + chr1_2:68529414-68529433 Msa0087560:five_prime_UTR 30.0%
!!! GCTCTCACCTTTAATTTTTT+AGG - chr1_2:68529687-68529706 None:intergenic 30.0%
!! TCTCAAAGTTTTGTACAAGA+AGG - chr1_2:68529902-68529921 None:intergenic 30.0%
!! TTCTAGTTTCTTATTCTCCT+CGG + chr1_2:68529930-68529949 Msa0087560:five_prime_UTR 30.0%
ACAAAGGTCGATCATAAAAA+AGG - chr1_2:68530005-68530024 None:intergenic 30.0%
! TGATCGACCTTTGTTATAAT+TGG + chr1_2:68530011-68530030 Msa0087560:five_prime_UTR 30.0%
GGTTATAACCAATTTATGCA+GGG - chr1_2:68530166-68530185 None:intergenic 30.0%
TGGTTATAACCAATTTATGC+AGG - chr1_2:68530167-68530186 None:intergenic 30.0%
GAATGGAACACAAATACTAT+TGG - chr1_2:68530187-68530206 None:intergenic 30.0%
!!! AGTATGATTTTGGGATTTGT+TGG + chr1_2:68530298-68530317 Msa0087560:exon 30.0%
TAGGCCAAAGAATATATACA+AGG + chr1_2:68530362-68530381 Msa0087560:CDS 30.0%
GGATCCTTGTATATATTCTT+TGG - chr1_2:68530369-68530388 None:intergenic 30.0%
CAAGGAATAAGTTGTGAAAA+AGG + chr1_2:68530580-68530599 Msa0087560:intron 30.0%
TAAGTTGTGAAAAAGGATGT+TGG + chr1_2:68530587-68530606 Msa0087560:intron 30.0%
TGAGAGAATATGATAGATGA+AGG - chr1_2:68530808-68530827 None:intergenic 30.0%
TCCTCAATACATCTATTAAG+CGG - chr1_2:68531080-68531099 None:intergenic 30.0%
TTACAATGTGTTTCAAGGAA+AGG - chr1_2:68531108-68531127 None:intergenic 30.0%
! GTTACTCTTTTGAACAGATT+TGG + chr1_2:68531252-68531271 Msa0087560:intron 30.0%
! GGCACTGAATTATGTTAAAT+AGG + chr1_2:68531435-68531454 Msa0087560:intron 30.0%
!!! GCTCATTTTTTATGAATGCT+AGG + chr1_2:68531588-68531607 Msa0087560:intron 30.0%
TCACTATTTACCTTATTGGT+AGG - chr1_2:68531680-68531699 None:intergenic 30.0%
TACAAAATCATGATTTCTCC+TGG + chr1_2:68531716-68531735 Msa0087560:intron 30.0%
! TACAACAAAATTTGTTCTCG+AGG - chr1_2:68531800-68531819 None:intergenic 30.0%
ATGTAATGTCTGTCACTAAT+AGG + chr1_2:68531971-68531990 Msa0087560:intron 30.0%
! TAATAGGTTCTTTGTTTGCT+TGG + chr1_2:68531987-68532006 Msa0087560:intron 30.0%
AGAAAGTGAGTGCTTTATAT+TGG + chr1_2:68532396-68532415 Msa0087560:exon 30.0%
!! TTCTGTGACCTATTTTAAGT+TGG + chr1_2:68532515-68532534 Msa0087560:three_prime_UTR 30.0%
!!! TCTGTGACCTATTTTAAGTT+GGG + chr1_2:68532516-68532535 Msa0087560:three_prime_UTR 30.0%
GGAGTATGTCTGTATTTAAA+TGG + chr1_2:68532694-68532713 Msa0087560:three_prime_UTR 30.0%
AGGGAAAACATTCATCACAA+AGG - chr1_2:68529445-68529464 None:intergenic 35.0%
TTGATCATGACTAATGAGTG+GGG - chr1_2:68529480-68529499 None:intergenic 35.0%
GTTGATCATGACTAATGAGT+GGG - chr1_2:68529481-68529500 None:intergenic 35.0%
AAGGTAATTGACAGGAAAAG+AGG - chr1_2:68529561-68529580 None:intergenic 35.0%
TAGAAACAAATGGACGAAAC+AGG - chr1_2:68529584-68529603 None:intergenic 35.0%
!! GTTTCGTCCATTTGTTTCTA+AGG + chr1_2:68529584-68529603 Msa0087560:five_prime_UTR 35.0%
GAGTATGAAATCTACAGGAA+GGG - chr1_2:68529613-68529632 None:intergenic 35.0%
AGAGTATGAAATCTACAGGA+AGG - chr1_2:68529614-68529633 None:intergenic 35.0%
ATTTCATACTCTCCCAAGAT+TGG + chr1_2:68529622-68529641 Msa0087560:five_prime_UTR 35.0%
TTTCATACTCTCCCAAGATT+GGG + chr1_2:68529623-68529642 Msa0087560:five_prime_UTR 35.0%
ATTAAAGGTGAGAGCTAAAC+AGG + chr1_2:68529692-68529711 Msa0087560:five_prime_UTR 35.0%
AAGGATGAACGATGTATCAA+GGG - chr1_2:68529883-68529902 None:intergenic 35.0%
TTTCTTATTCTCCTCGGATT+GGG + chr1_2:68529936-68529955 Msa0087560:five_prime_UTR 35.0%
AAAAGAGCAGACTGATCAAA+GGG - chr1_2:68529979-68529998 None:intergenic 35.0%
TATAACCAATTTATGCAGGG+TGG - chr1_2:68530163-68530182 None:intergenic 35.0%
ATATACAAGGATCCAGATGA+TGG + chr1_2:68530375-68530394 Msa0087560:CDS 35.0%
TATACAAGGATCCAGATGAT+GGG + chr1_2:68530376-68530395 Msa0087560:CDS 35.0%
TTAAGCAATAGCTGCATATG+TGG + chr1_2:68530508-68530527 Msa0087560:intron 35.0%
TGTCGAGTCATGTATTTGAA+AGG + chr1_2:68530532-68530551 Msa0087560:intron 35.0%
GTCGAGTCATGTATTTGAAA+GGG + chr1_2:68530533-68530552 Msa0087560:intron 35.0%
! TCTAGAGTAGATTAGGTGTT+TGG + chr1_2:68530648-68530667 Msa0087560:intron 35.0%
CAAGTACATGGTCTGTTTAA+CGG + chr1_2:68530685-68530704 Msa0087560:intron 35.0%
AAGTACATGGTCTGTTTAAC+GGG + chr1_2:68530686-68530705 Msa0087560:intron 35.0%
GACATTGAGTTTAGTGACAT+TGG + chr1_2:68530721-68530740 Msa0087560:intron 35.0%
! ACATTGAGCTTTTGTGACAT+CGG + chr1_2:68530889-68530908 Msa0087560:intron 35.0%
TCCGCTTAATAGATGTATTG+AGG + chr1_2:68531076-68531095 Msa0087560:intron 35.0%
ATGCTTCGTCCTCAAAATAT+CGG - chr1_2:68531285-68531304 None:intergenic 35.0%
! TAGCGATGCTTTGTTCAAAT+GGG + chr1_2:68531547-68531566 Msa0087560:intron 35.0%
GAAGAAGCTCAAGAAAATAG+AGG - chr1_2:68531625-68531644 None:intergenic 35.0%
! TGCAAATTTTCGCAATGCAA+TGG + chr1_2:68531649-68531668 Msa0087560:CDS 35.0%
TTGAGACAGATAGACAGATA+TGG + chr1_2:68531842-68531861 Msa0087560:intron 35.0%
TACCTACTGCATCACAATTT+CGG - chr1_2:68531908-68531927 None:intergenic 35.0%
TTTCTTTGCCTTGTGTTTCT+TGG + chr1_2:68532065-68532084 Msa0087560:intron 35.0%
! TCTACTTCTGGAAGAACTAT+AGG + chr1_2:68532136-68532155 Msa0087560:CDS 35.0%
GGAAGAACTATAGGAACAAT+CGG + chr1_2:68532145-68532164 Msa0087560:CDS 35.0%
!!! CATGTCATTTTTTGCACTAC+CGG - chr1_2:68532349-68532368 None:intergenic 35.0%
GAAATACTTCAGCTGATAGA+AGG + chr1_2:68532370-68532389 Msa0087560:CDS 35.0%
TGTGCTACCCAACTTAAAAT+AGG - chr1_2:68532526-68532545 None:intergenic 35.0%
GGTTGATCATGACTAATGAG+TGG - chr1_2:68529482-68529501 None:intergenic 40.0%
ACAGGAAAAGAGGCTAATGA+TGG - chr1_2:68529551-68529570 None:intergenic 40.0%
GAAACAGGAAGGTAATTGAC+AGG - chr1_2:68529569-68529588 None:intergenic 40.0%
AACAAATGGACGAAACAGGA+AGG - chr1_2:68529580-68529599 None:intergenic 40.0%
AGGGAAGCCTTAGAAACAAA+TGG - chr1_2:68529594-68529613 None:intergenic 40.0%
! TGGGAGAGTATGAAATCTAC+AGG - chr1_2:68529618-68529637 None:intergenic 40.0%
GTATCAAAGCTCCCAATCTT+GGG - chr1_2:68529637-68529656 None:intergenic 40.0%
TGTATCAAAGCTCCCAATCT+TGG - chr1_2:68529638-68529657 None:intergenic 40.0%
AAACAGGAAAGGTGGAATGA+CGG + chr1_2:68529708-68529727 Msa0087560:five_prime_UTR 40.0%
ATCTATTGTGCAGCACTTGT+TGG - chr1_2:68529757-68529776 None:intergenic 40.0%
AGAAGGGACATATGCCAATT+GGG - chr1_2:68529806-68529825 None:intergenic 40.0%
TTAGAGGAGGAATGACAGAA+GGG - chr1_2:68529822-68529841 None:intergenic 40.0%
ACGGATAAAACAGTTAGAGG+AGG - chr1_2:68529835-68529854 None:intergenic 40.0%
GAGACGGATAAAACAGTTAG+AGG - chr1_2:68529838-68529857 None:intergenic 40.0%
AGGATGAACGATGTATCAAG+GGG - chr1_2:68529882-68529901 None:intergenic 40.0%
GAAGGATGAACGATGTATCA+AGG - chr1_2:68529884-68529903 None:intergenic 40.0%
GTTTCTTATTCTCCTCGGAT+TGG + chr1_2:68529935-68529954 Msa0087560:five_prime_UTR 40.0%
CAAAAGAGCAGACTGATCAA+AGG - chr1_2:68529980-68529999 None:intergenic 40.0%
TGGTGCAAAAAGATAAGGAG+AGG + chr1_2:68530066-68530085 Msa0087560:five_prime_UTR 40.0%
! CTTCCTTTGAAAAACCAACC+AGG - chr1_2:68530132-68530151 None:intergenic 40.0%
ATACGGAAAGCCCTTGAAAA+GGG - chr1_2:68530225-68530244 None:intergenic 40.0%
TATACGGAAAGCCCTTGAAA+AGG - chr1_2:68530226-68530245 None:intergenic 40.0%
!!! GCTGATGGTTGTTTTTCTCT+AGG + chr1_2:68530343-68530362 Msa0087560:CDS 40.0%
GAAAACCATACAGTGGATGT+AGG - chr1_2:68530454-68530473 None:intergenic 40.0%
TAAAGTCACCGATGTCACAA+AGG + chr1_2:68530839-68530858 Msa0087560:intron 40.0%
ACATTGAGCCTTTGTGACAT+CGG - chr1_2:68530850-68530869 None:intergenic 40.0%
TCAATGTCACGGAAACTACT+TGG + chr1_2:68530863-68530882 Msa0087560:intron 40.0%
CAATGTCACGGAAACTACTT+GGG + chr1_2:68530864-68530883 Msa0087560:intron 40.0%
CAAAAAAGGGGAATTTCACC+TGG - chr1_2:68531142-68531161 None:intergenic 40.0%
! TTTGAGGACGAAGCATTCAT+TGG + chr1_2:68531289-68531308 Msa0087560:CDS 40.0%
! TTGAGGACGAAGCATTCATT+GGG + chr1_2:68531290-68531309 Msa0087560:CDS 40.0%
CTTATTGTCCCACTAAAGTC+TGG + chr1_2:68531380-68531399 Msa0087560:intron 40.0%
! CTAGCGATGCTTTGTTCAAA+TGG + chr1_2:68531546-68531565 Msa0087560:intron 40.0%
TCAAGAAAATAGAGGCAGTG+AGG - chr1_2:68531617-68531636 None:intergenic 40.0%
AATGGCACATCCTACCAATA+AGG + chr1_2:68531667-68531686 Msa0087560:CDS 40.0%
TACCGAAATTGTGATGCAGT+AGG + chr1_2:68531903-68531922 Msa0087560:intron 40.0%
TGGTCAATCCAAGAAACACA+AGG - chr1_2:68532076-68532095 None:intergenic 40.0%
ATAGACAGCAGTTCTACTTC+TGG + chr1_2:68532124-68532143 Msa0087560:CDS 40.0%
ATACTTTCTTCCATCGCAGA+TGG + chr1_2:68532427-68532446 Msa0087560:three_prime_UTR 40.0%
TACTTTCTTCCATCGCAGAT+GGG + chr1_2:68532428-68532447 Msa0087560:three_prime_UTR 40.0%
AACCCTTGTATGACAAGCCA+CGG + chr1_2:68529498-68529517 Msa0087560:five_prime_UTR 45.0%
AGGTGAGAGCTAAACAGGAA+AGG + chr1_2:68529697-68529716 Msa0087560:five_prime_UTR 45.0%
TGAGAGCTAAACAGGAAAGG+TGG + chr1_2:68529700-68529719 Msa0087560:five_prime_UTR 45.0%
GAAGGGACATATGCCAATTG+GGG - chr1_2:68529805-68529824 None:intergenic 45.0%
CAGAAGGGACATATGCCAAT+TGG - chr1_2:68529807-68529826 None:intergenic 45.0%
GTTAGAGGAGGAATGACAGA+AGG - chr1_2:68529823-68529842 None:intergenic 45.0%
!! TAAGGAGAGGCACTTTATGC+TGG + chr1_2:68530079-68530098 Msa0087560:five_prime_UTR 45.0%
GTTATGAGTGTAAGCCAACC+AGG + chr1_2:68530106-68530125 Msa0087560:five_prime_UTR 45.0%
TTTGAAAAACCAACCAGGCC+TGG - chr1_2:68530127-68530146 None:intergenic 45.0%
!!! AGGCCTGGTTGGTTTTTCAA+AGG + chr1_2:68530126-68530145 Msa0087560:five_prime_UTR 45.0%
TCAAGCCACCCTGCATAAAT+TGG + chr1_2:68530155-68530174 Msa0087560:five_prime_UTR 45.0%
! TGAAGTGTGTGCCCTTTTCA+AGG + chr1_2:68530211-68530230 Msa0087560:five_prime_UTR 45.0%
! GAAGTGTGTGCCCTTTTCAA+GGG + chr1_2:68530212-68530231 Msa0087560:five_prime_UTR 45.0%
! ACAACGGTTTTTGCTGGAAC+TGG + chr1_2:68530401-68530420 Msa0087560:CDS 45.0%
! GGATGTAGGTAGGATTAGCA+AGG - chr1_2:68530440-68530459 None:intergenic 45.0%
TCCTACCTACATCCACTGTA+TGG + chr1_2:68530446-68530465 Msa0087560:intron 45.0%
ACCATACAGTGGATGTAGGT+AGG - chr1_2:68530450-68530469 None:intergenic 45.0%
!! TCTGTTTAACGGGTTGCACT+TGG + chr1_2:68530696-68530715 Msa0087560:intron 45.0%
GTCACAAAGGCTCAATGTCA+CGG + chr1_2:68530852-68530871 Msa0087560:intron 45.0%
TGTGACATCGGTGACTTTAG+TGG + chr1_2:68530901-68530920 Msa0087560:intron 45.0%
GAGTTGCACTTGGATGACAT+TGG - chr1_2:68531011-68531030 None:intergenic 45.0%
GGAAACTACTTGGGTGACAT+TGG - chr1_2:68530873-68530892 None:intergenic 45.0%
ACCCAAGTAGTTTCCCTGTT+GGG + chr1_2:68531038-68531057 Msa0087560:intron 45.0%
ACCCAACAGGGAAACTACTT+GGG - chr1_2:68531042-68531061 None:intergenic 45.0%
AACCCAACAGGGAAACTACT+TGG - chr1_2:68531043-68531062 None:intergenic 45.0%
CATGCTTTGTCATTGCTGCT+TGG + chr1_2:68531176-68531195 Msa0087560:CDS 45.0%
! GGCTCAGAACCGATATTTTG+AGG + chr1_2:68531273-68531292 Msa0087560:CDS 45.0%
TCCCACTAAAGTCTGGAAGA+GGG + chr1_2:68531387-68531406 Msa0087560:intron 45.0%
TCCCTCTTCCAGACTTTAGT+GGG - chr1_2:68531391-68531410 None:intergenic 45.0%
ATCCCTCTTCCAGACTTTAG+TGG - chr1_2:68531392-68531411 None:intergenic 45.0%
! CGATGCTTTGTTCAAATGGG+TGG + chr1_2:68531550-68531569 Msa0087560:intron 45.0%
! ATACCGTATGGCATTGGAGA+AGG - chr1_2:68532315-68532334 None:intergenic 45.0%
TCTAACGAGTTTCAACCGTC+TGG - chr1_2:68532570-68532589 None:intergenic 45.0%
TAATGAGTGGGGAGTCACAG+AGG - chr1_2:68529469-68529488 None:intergenic 50.0%
! AGCCGTGGCTTGTCATACAA+GGG - chr1_2:68529503-68529522 None:intergenic 50.0%
TTATCGCTCCACTCCCCAAT+TGG + chr1_2:68529789-68529808 Msa0087560:five_prime_UTR 50.0%
GACATATGCCAATTGGGGAG+TGG - chr1_2:68529800-68529819 None:intergenic 50.0%
TGCTGTCTAAACCCAATCCG+AGG - chr1_2:68529950-68529969 None:intergenic 50.0%
AAAAACCAACCAGGCCTGGT+TGG - chr1_2:68530123-68530142 None:intergenic 50.0%
GCGCTTAAGTACAGCTCAAC+AGG - chr1_2:68530325-68530344 None:intergenic 50.0%
!! GAGCTGTACTTAAGCGCTGA+TGG + chr1_2:68530328-68530347 Msa0087560:CDS 50.0%
!! ATCCAGATGATGGGCGACAA+CGG + chr1_2:68530385-68530404 Msa0087560:CDS 50.0%
GGTGACATTGGAGTTGCACT+TGG - chr1_2:68531021-68531040 None:intergenic 50.0%
CACCCAAGTAGTTTCCCTGT+TGG + chr1_2:68531037-68531056 Msa0087560:intron 50.0%
GTCCCACTAAAGTCTGGAAG+AGG + chr1_2:68531386-68531405 Msa0087560:intron 50.0%
!! CTAGGTTCTGGAAGTGGTCT+TGG - chr1_2:68532186-68532205 None:intergenic 50.0%
!! ACACTTGTAGCAGGTACTGG+TGG - chr1_2:68532264-68532283 None:intergenic 50.0%
GCAACACTTGTAGCAGGTAC+TGG - chr1_2:68532267-68532286 None:intergenic 50.0%
GTCACAGCAACACTTGTAGC+AGG - chr1_2:68532273-68532292 None:intergenic 50.0%
GCTCCTTCTCCAATGCCATA+CGG + chr1_2:68532309-68532328 Msa0087560:CDS 50.0%
! TTTTTGCACTACCGGAGCCA+GGG - chr1_2:68532341-68532360 None:intergenic 50.0%
!!! TTTTTTGCACTACCGGAGCC+AGG - chr1_2:68532342-68532361 None:intergenic 50.0%
ACTTCAACTCCCATCTGCGA+TGG - chr1_2:68532440-68532459 None:intergenic 50.0%
AGTGGGGAGTCACAGAGGAA+GGG - chr1_2:68529464-68529483 None:intergenic 55.0%
! CAGCCGTGGCTTGTCATACA+AGG - chr1_2:68529504-68529523 None:intergenic 55.0%
GGCACACACTTCACGCTGAA+TGG - chr1_2:68530204-68530223 None:intergenic 55.0%
AACCGTTGTCGCCCATCATC+TGG - chr1_2:68530390-68530409 None:intergenic 55.0%
! TGGGCGACAACGGTTTTTGC+TGG + chr1_2:68530395-68530414 Msa0087560:CDS 55.0%
CCTCTCAGTGCAACCCAACA+GGG - chr1_2:68531054-68531073 None:intergenic 55.0%
! CCTGTTGGGTTGCACTGAGA+GGG + chr1_2:68531052-68531071 Msa0087560:intron 55.0%
!! GCAGCACTAGGTTCTGGAAG+TGG - chr1_2:68532192-68532211 None:intergenic 55.0%
TAGTGCATGCACAGGTGGCT+GGG - chr1_2:68532241-68532260 None:intergenic 55.0%
! ACTGGTGGTAGTGCATGCAC+AGG - chr1_2:68532249-68532268 None:intergenic 55.0%
! GGGGGTATACCGTATGGCAT+TGG - chr1_2:68532321-68532340 None:intergenic 55.0%
ATGCCATACGGTATACCCCC+TGG + chr1_2:68532321-68532340 Msa0087560:CDS 55.0%
! TTTTGCACTACCGGAGCCAG+GGG - chr1_2:68532340-68532359 None:intergenic 55.0%
GAGTGGGGAGTCACAGAGGA+AGG - chr1_2:68529465-68529484 None:intergenic 60.0%
GAGTGTAAGCCAACCAGGCC+TGG + chr1_2:68530111-68530130 Msa0087560:five_prime_UTR 60.0%
GTAAGCCAACCAGGCCTGGT+TGG + chr1_2:68530115-68530134 Msa0087560:five_prime_UTR 60.0%
! CCCTGTTGGGTTGCACTGAG+AGG + chr1_2:68531051-68531070 Msa0087560:intron 60.0%
CCCTCTCAGTGCAACCCAAC+AGG - chr1_2:68531055-68531074 None:intergenic 60.0%
! GGAAGCGCAGCACTAGGTTC+TGG - chr1_2:68532198-68532217 None:intergenic 60.0%
GAACCTAGTGCTGCGCTTCC+TGG + chr1_2:68532198-68532217 Msa0087560:CDS 60.0%
TAGTGCTGCGCTTCCTGGTC+CGG + chr1_2:68532203-68532222 Msa0087560:CDS 60.0%
AGTGCATGCACAGGTGGCTG+GGG - chr1_2:68532240-68532259 None:intergenic 60.0%
GTAGTGCATGCACAGGTGGC+TGG - chr1_2:68532242-68532261 None:intergenic 60.0%
! GGTGGTAGTGCATGCACAGG+TGG - chr1_2:68532246-68532265 None:intergenic 60.0%
! GAGCCAGGGGGTATACCGTA+TGG - chr1_2:68532327-68532346 None:intergenic 60.0%
TACGGTATACCCCCTGGCTC+CGG + chr1_2:68532327-68532346 Msa0087560:CDS 60.0%
TTTGCACTACCGGAGCCAGG+GGG - chr1_2:68532339-68532358 None:intergenic 60.0%
GCTTGCAGTGAGAGCAGCCG+TGG - chr1_2:68529518-68529537 None:intergenic 65.0%
! GGACCAGGAAGCGCAGCACT+AGG - chr1_2:68532204-68532223 None:intergenic 65.0%
AGGTGGCTGGGGTTGAGTCG+AGG - chr1_2:68532229-68532248 None:intergenic 65.0%
GGTTGAGTCGAGGCCGGACC+AGG - chr1_2:68532219-68532238 None:intergenic 70.0%
GGCTGGGGTTGAGTCGAGGC+CGG - chr1_2:68532225-68532244 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 68529386 68532736 68529386 ID=Msa0087560;Name=Msa0087560
chr1_2 mRNA 68529386 68532736 68529386 ID=Msa0087560-mRNA-1;Parent=Msa0087560;Name=Msa0087560-mRNA-1;_AED=0.30;_eAED=0.30;_QI=915|0.5|0.8|0.8|0.5|0.8|5|332|227
chr1_2 exon 68529386 68530462 68529386 ID=Msa0087560-mRNA-1:exon:23396;Parent=Msa0087560-mRNA-1
chr1_2 exon 68531143 68531197 68531143 ID=Msa0087560-mRNA-1:exon:23397;Parent=Msa0087560-mRNA-1
chr1_2 exon 68531269 68531365 68531269 ID=Msa0087560-mRNA-1:exon:23398;Parent=Msa0087560-mRNA-1
chr1_2 exon 68531610 68531688 68531610 ID=Msa0087560-mRNA-1:exon:23399;Parent=Msa0087560-mRNA-1
chr1_2 exon 68532114 68532736 68532114 ID=Msa0087560-mRNA-1:exon:23400;Parent=Msa0087560-mRNA-1
chr1_2 five_prime_UTR 68529386 68530300 68529386 ID=Msa0087560-mRNA-1:five_prime_utr;Parent=Msa0087560-mRNA-1
chr1_2 CDS 68530301 68530462 68530301 ID=Msa0087560-mRNA-1:cds;Parent=Msa0087560-mRNA-1
chr1_2 CDS 68531143 68531197 68531143 ID=Msa0087560-mRNA-1:cds;Parent=Msa0087560-mRNA-1
chr1_2 CDS 68531269 68531365 68531269 ID=Msa0087560-mRNA-1:cds;Parent=Msa0087560-mRNA-1
chr1_2 CDS 68531610 68531688 68531610 ID=Msa0087560-mRNA-1:cds;Parent=Msa0087560-mRNA-1
chr1_2 CDS 68532114 68532404 68532114 ID=Msa0087560-mRNA-1:cds;Parent=Msa0087560-mRNA-1
chr1_2 three_prime_UTR 68532405 68532736 68532405 ID=Msa0087560-mRNA-1:three_prime_utr;Parent=Msa0087560-mRNA-1
Gene Sequence

>Msa0087560

ATGATTTTGGGATTTGTTGGACCTGTTGAGCTGTACTTAAGCGCTGATGGTTGTTTTTCTCTAGGCCAAAGAATATATACAAGGATCCAGATGATGGGCGACAACGGTTTTTGCTGGAACTGGAATTTGTTCAATGCCTTGCTAATCCTACCTACATCCACTGTGAAATTCCCCTTTTTTGACAAAAAAACATGGCATGCTTTGTCATTGCTGCTTGATTTGGCTCAGAACCGATATTTTGAGGACGAAGCATTCATTGGGTACTTAAAGTATCTTCAATATTGGCAACGCCCTGAATATATTAAATTTATCATGTATCCTCACTGCCTCTATTTTCTTGAGCTTCTTCAGAATGCAAATTTTCGCAATGCAATGGCACATCCTACCAATAAGGAATTGACACATAGACAGCAGTTCTACTTCTGGAAGAACTATAGGAACAATCGGCTAAAACACATTTTACCAAGACCACTTCCAGAACCTAGTGCTGCGCTTCCTGGTCCGGCCTCGACTCAACCCCAGCCACCTGTGCATGCACTACCACCAGTACCTGCTACAAGTGTTGCTGTGACAACATCGTCTAGTCAAGCTCCTTCTCCAATGCCATACGGTATACCCCCTGGCTCCGGTAGTGCAAAAAATGACATGAGAAATACTTCAGCTGATAGAAGGAAAAGAAAGTGA
Protein sequence

>Msa0087560

MILGFVGPVELYLSADGCFSLGQRIYTRIQMMGDNGFCWNWNLFNALLILPTSTVKFPFFDKKTWHALSLLLDLAQNRYFEDEAFIGYLKYLQYWQRPEYIKFIMYPHCLYFLELLQNANFRNAMAHPTNKELTHRQQFYFWKNYRNNRLKHILPRPLPEPSAALPGPASTQPQPPVHALPPVPATSVAVTTSSSQAPSPMPYGIPPGSGSAKNDMRNTSADRRKRK*