AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0134280

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0134280 MtrunA17_Chr1g0204501 96.753 154 5 0 74 227 45 198 4.35e-107 306
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0134280 AT5G19910.1 68.790 157 32 4 74 227 51 193 1.99e-57 181
Msa0134280 AT5G19910.2 57.754 187 32 5 74 227 51 223 9.33e-51 165

Find 128 sgRNAs with CRISPR-Local

Find 239 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTAATAGAAGTATGATTT+TGG 0.136395 1_3:+65822616 Msa0134280:exon
GATATCACTATTTACCTTAT+TGG 0.153158 1_3:-65824009 None:intergenic
TGATCGACCTTTGTTATAAT+TGG 0.166073 1_3:+65822339 Msa0134280:five_prime_UTR
GTAGTGCATGCACAGGTGGC+TGG 0.230176 1_3:-65824567 None:intergenic
GTTTCGTCCATTTGTTTCTA+AGG 0.240006 1_3:+65821912 Msa0134280:five_prime_UTR
GGAGTTGAAGTAATAATAAT+AGG 0.256428 1_3:+65824777 Msa0134280:three_prime_UTR
GGATCCTTGTATATATTCTT+TGG 0.257913 1_3:-65822694 None:intergenic
TCAAGCCACCCTGCATAAAT+TGG 0.272984 1_3:+65822483 Msa0134280:five_prime_UTR
GAACCTAGTGCTGCGCTTCC+TGG 0.281659 1_3:+65824526 Msa0134280:CDS
CAGTTTGAAAATTTAATATA+TGG 0.284142 1_3:+65822374 Msa0134280:five_prime_UTR
AGAAAGTGAGTGCTTTATAT+TGG 0.289671 1_3:+65824724 Msa0134280:exon
ACTTAAAGTATCTTCAATAT+TGG 0.293258 1_3:+65823642 Msa0134280:CDS
TTTGTTATAATTGGCATATC+TGG 0.303686 1_3:+65822348 Msa0134280:five_prime_UTR
ATCTATTGTGCAGCACTTGT+TGG 0.309094 1_3:-65822082 None:intergenic
TATTTAAATGGTACAAGCTT+TGG 0.323173 1_3:+65825034 Msa0134280:three_prime_UTR
CATGCTTTGTCATTGCTGCT+TGG 0.324180 1_3:+65823504 Msa0134280:CDS
TGTATCAAAGCTCCCAATCT+TGG 0.330312 1_3:-65821963 None:intergenic
ATGATAAATTTAATATATTC+AGG 0.332776 1_3:-65823671 None:intergenic
GGAGTATGTCTGTATTTAAA+TGG 0.347406 1_3:+65825022 Msa0134280:three_prime_UTR
AACCGTTGTCGCCCATCATC+TGG 0.352900 1_3:-65822715 None:intergenic
GTTTCTTATTCTCCTCGGAT+TGG 0.357717 1_3:+65822263 Msa0134280:five_prime_UTR
ATTAAAGGTGAGAGCTAAAC+AGG 0.361539 1_3:+65822020 Msa0134280:five_prime_UTR
AAATTAAAATCTGTAGTATA+CGG 0.364774 1_3:-65822567 None:intergenic
ATTTCATACTCTCCCAAGAT+TGG 0.365797 1_3:+65821950 Msa0134280:five_prime_UTR
CTAGGTTCTGGAAGTGGTCT+TGG 0.382881 1_3:-65824511 None:intergenic
TAGTGCATGCACAGGTGGCT+GGG 0.384524 1_3:-65824566 None:intergenic
TTTCTTATTCTCCTCGGATT+GGG 0.385548 1_3:+65822264 Msa0134280:five_prime_UTR
AATGGCACATCCTACCAATA+AGG 0.389767 1_3:+65823995 Msa0134280:CDS
GGAAGCGCAGCACTAGGTTC+TGG 0.393441 1_3:-65824523 None:intergenic
TGTGCTACCCAACTTAAAAT+AGG 0.393795 1_3:-65824851 None:intergenic
AGAAGGGACATATGCCAATT+GGG 0.399848 1_3:-65822131 None:intergenic
CAGAAGGGACATATGCCAAT+TGG 0.400766 1_3:-65822132 None:intergenic
TTCTAGTTTCTTATTCTCCT+CGG 0.403178 1_3:+65822258 Msa0134280:five_prime_UTR
TCTACTTCTGGAAGAACTAT+AGG 0.406034 1_3:+65824464 Msa0134280:CDS
GAAATACTTCAGCTGATAGA+AGG 0.409099 1_3:+65824698 Msa0134280:CDS
TTATCGCTCCACTCCCCAAT+TGG 0.410613 1_3:+65822117 Msa0134280:five_prime_UTR
TTTGAGGACGAAGCATTCAT+TGG 0.411048 1_3:+65823617 Msa0134280:CDS
GCGCTTAAGTACAGCTCAAC+AGG 0.415512 1_3:-65822650 None:intergenic
GAATGGAACACAAATACTAT+TGG 0.422824 1_3:-65822512 None:intergenic
GAGACGGATAAAACAGTTAG+AGG 0.430861 1_3:-65822163 None:intergenic
AAATTTGATAAAACAGAGAT+TGG 0.431232 1_3:+65821985 Msa0134280:five_prime_UTR
GGCACACACTTCACGCTGAA+TGG 0.436638 1_3:-65822529 None:intergenic
TGGTTATAACCAATTTATGC+AGG 0.437509 1_3:-65822492 None:intergenic
GTATCAAAGCTCCCAATCTT+GGG 0.440002 1_3:-65821962 None:intergenic
ATGCTTCGTCCTCAAAATAT+CGG 0.443322 1_3:-65823610 None:intergenic
TAGAAACAAATGGACGAAAC+AGG 0.452632 1_3:-65821909 None:intergenic
TAGTGCTGCGCTTCCTGGTC+CGG 0.453206 1_3:+65824531 Msa0134280:CDS
ATAGACAGCAGTTCTACTTC+TGG 0.455964 1_3:+65824452 Msa0134280:CDS
AGTGCATGCACAGGTGGCTG+GGG 0.456575 1_3:-65824565 None:intergenic
GGTTGAGTCGAGGCCGGACC+AGG 0.460454 1_3:-65824544 None:intergenic
TTTCATACTCTCCCAAGATT+GGG 0.461521 1_3:+65821951 Msa0134280:five_prime_UTR
CAGCCGTGGCTTGTCATACA+AGG 0.470099 1_3:-65821829 None:intergenic
GGCTGGGGTTGAGTCGAGGC+CGG 0.471060 1_3:-65824550 None:intergenic
TAAGGAGAGGCACTTTATGC+TGG 0.471146 1_3:+65822407 Msa0134280:five_prime_UTR
GAGTGTAAGCCAACCAGGCC+TGG 0.473699 1_3:+65822439 Msa0134280:five_prime_UTR
TCACTATTTACCTTATTGGT+AGG 0.479734 1_3:-65824005 None:intergenic
AGGGAAGCCTTAGAAACAAA+TGG 0.488857 1_3:-65821919 None:intergenic
TATACGGAAAGCCCTTGAAA+AGG 0.490993 1_3:-65822551 None:intergenic
TGATAAATTTAATATATTCA+GGG 0.495109 1_3:-65823670 None:intergenic
GAAGGATGAACGATGTATCA+AGG 0.498494 1_3:-65822209 None:intergenic
GGAAGAACTATAGGAACAAT+CGG 0.500395 1_3:+65824473 Msa0134280:CDS
TGGGAGAGTATGAAATCTAC+AGG 0.502485 1_3:-65821943 None:intergenic
GAGTTGAAGTAATAATAATA+GGG 0.503829 1_3:+65824778 Msa0134280:three_prime_UTR
TTGAGGACGAAGCATTCATT+GGG 0.512149 1_3:+65823618 Msa0134280:CDS
TCTAACGAGTTTCAACCGTC+TGG 0.512281 1_3:-65824895 None:intergenic
ATACTTTCTTCCATCGCAGA+TGG 0.517896 1_3:+65824755 Msa0134280:three_prime_UTR
GCAACACTTGTAGCAGGTAC+TGG 0.519969 1_3:-65824592 None:intergenic
GAAGAAGCTCAAGAAAATAG+AGG 0.520705 1_3:-65823950 None:intergenic
GAGTGGGGAGTCACAGAGGA+AGG 0.522359 1_3:-65821790 None:intergenic
AAAATGAAATTGATCAAGAC+AGG 0.523673 1_3:-65822592 None:intergenic
ATATACAAGGATCCAGATGA+TGG 0.525951 1_3:+65822703 Msa0134280:CDS
AAGGTAATTGACAGGAAAAG+AGG 0.529830 1_3:-65821886 None:intergenic
AAAAGAGCAGACTGATCAAA+GGG 0.534798 1_3:-65822304 None:intergenic
GGTTATAACCAATTTATGCA+GGG 0.536876 1_3:-65822491 None:intergenic
GTTATGAGTGTAAGCCAACC+AGG 0.539396 1_3:+65822434 Msa0134280:five_prime_UTR
ACAGGAAAAGAGGCTAATGA+TGG 0.543975 1_3:-65821876 None:intergenic
AAGGATGAACGATGTATCAA+GGG 0.547169 1_3:-65822208 None:intergenic
GAGCTGTACTTAAGCGCTGA+TGG 0.548171 1_3:+65822656 Msa0134280:CDS
GGACCAGGAAGCGCAGCACT+AGG 0.549090 1_3:-65824529 None:intergenic
GCAGCACTAGGTTCTGGAAG+TGG 0.553833 1_3:-65824517 None:intergenic
ATACGGAAAGCCCTTGAAAA+GGG 0.555379 1_3:-65822550 None:intergenic
CAAAAGAGCAGACTGATCAA+AGG 0.567861 1_3:-65822305 None:intergenic
ATACCGTATGGCATTGGAGA+AGG 0.568373 1_3:-65824640 None:intergenic
TACTTTCTTCCATCGCAGAT+GGG 0.571187 1_3:+65824756 Msa0134280:three_prime_UTR
GACATATGCCAATTGGGGAG+TGG 0.571738 1_3:-65822125 None:intergenic
GAAAACCATACAGTGGATGT+AGG 0.571854 1_3:-65822779 None:intergenic
GTAAGCCAACCAGGCCTGGT+TGG 0.572231 1_3:+65822443 Msa0134280:five_prime_UTR
AGGTGGCTGGGGTTGAGTCG+AGG 0.574599 1_3:-65824554 None:intergenic
GTTGATCATGACTAATGAGT+GGG 0.575424 1_3:-65821806 None:intergenic
AACCCTTGTATGACAAGCCA+CGG 0.581906 1_3:+65821826 Msa0134280:five_prime_UTR
GAAACAGGAAGGTAATTGAC+AGG 0.583410 1_3:-65821894 None:intergenic
AGAGTATGAAATCTACAGGA+AGG 0.585843 1_3:-65821939 None:intergenic
GTTAGAGGAGGAATGACAGA+AGG 0.586720 1_3:-65822148 None:intergenic
GTCACAGCAACACTTGTAGC+AGG 0.586975 1_3:-65824598 None:intergenic
ACCATACAGTGGATGTAGGT+AGG 0.594759 1_3:-65822775 None:intergenic
ATCCAGATGATGGGCGACAA+CGG 0.595215 1_3:+65822713 Msa0134280:CDS
GCTCCTTCTCCAATGCCATA+CGG 0.595355 1_3:+65824637 Msa0134280:CDS
GCTTGCAGTGAGAGCAGCCG+TGG 0.596439 1_3:-65821843 None:intergenic
ACTTCAACTCCCATCTGCGA+TGG 0.598476 1_3:-65824765 None:intergenic
TTGATGTTACAAATGATGAA+AGG 0.600431 1_3:+65825001 Msa0134280:three_prime_UTR
GGTGGTAGTGCATGCACAGG+TGG 0.607758 1_3:-65824571 None:intergenic
TATACAAGGATCCAGATGAT+GGG 0.614053 1_3:+65822704 Msa0134280:CDS
GAGTATGAAATCTACAGGAA+GGG 0.616379 1_3:-65821938 None:intergenic
AAACAGGAAAGGTGGAATGA+CGG 0.625544 1_3:+65822036 Msa0134280:five_prime_UTR
AGATATGCCAATTATAACAA+AGG 0.628476 1_3:-65822346 None:intergenic
ACGGATAAAACAGTTAGAGG+AGG 0.632952 1_3:-65822160 None:intergenic
TCCATATAGTTAAAGGCGGG+GGG 0.633111 1_3:+65821699 None:intergenic
TGAGAGCTAAACAGGAAAGG+TGG 0.639079 1_3:+65822028 Msa0134280:five_prime_UTR
AACAAATGGACGAAACAGGA+AGG 0.640927 1_3:-65821905 None:intergenic
TAGGCCAAAGAATATATACA+AGG 0.644719 1_3:+65822690 Msa0134280:CDS
GAAGGGACATATGCCAATTG+GGG 0.644738 1_3:-65822130 None:intergenic
TCAAGAAAATAGAGGCAGTG+AGG 0.646000 1_3:-65823942 None:intergenic
TTAGAGGAGGAATGACAGAA+GGG 0.646052 1_3:-65822147 None:intergenic
GGTTGATCATGACTAATGAG+TGG 0.646535 1_3:-65821807 None:intergenic
AGTGGGGAGTCACAGAGGAA+GGG 0.648009 1_3:-65821789 None:intergenic
AGGTGAGAGCTAAACAGGAA+AGG 0.648812 1_3:+65822025 Msa0134280:five_prime_UTR
TATAACCAATTTATGCAGGG+TGG 0.649169 1_3:-65822488 None:intergenic
GGATGTAGGTAGGATTAGCA+AGG 0.649374 1_3:-65822765 None:intergenic
ATTGAAAATACATATCCAGA+CGG 0.649827 1_3:+65824880 Msa0134280:three_prime_UTR
ACTGGTGGTAGTGCATGCAC+AGG 0.653824 1_3:-65824574 None:intergenic
TGCTGTCTAAACCCAATCCG+AGG 0.660940 1_3:-65822275 None:intergenic
AGGGAAAACATTCATCACAA+AGG 0.672318 1_3:-65821770 None:intergenic
AGGATGAACGATGTATCAAG+GGG 0.675967 1_3:-65822207 None:intergenic
TAATGAGTGGGGAGTCACAG+AGG 0.683280 1_3:-65821794 None:intergenic
AGCCGTGGCTTGTCATACAA+GGG 0.688236 1_3:-65821828 None:intergenic
ACACTTGTAGCAGGTACTGG+TGG 0.693490 1_3:-65824589 None:intergenic
TTGATCATGACTAATGAGTG+GGG 0.702989 1_3:-65821805 None:intergenic
TTTGCACTACCGGAGCCAGG+GGG 0.746793 1_3:-65824664 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGATAAATTTAATATATTCA+GGG - chr1_3:65823673-65823692 None:intergenic 10.0%
!! ATGATAAATTTAATATATTC+AGG - chr1_3:65823674-65823693 None:intergenic 10.0%
!!! AATTTCAAATTATCTTTTAA+TGG + chr1_3:65824950-65824969 Msa0134280:three_prime_UTR 10.0%
!! CAGTTTGAAAATTTAATATA+TGG + chr1_3:65822374-65822393 Msa0134280:five_prime_UTR 15.0%
!! AAATTAAAATCTGTAGTATA+CGG - chr1_3:65822570-65822589 None:intergenic 15.0%
!!! ATTAATAGAAGTATGATTTT+GGG + chr1_3:65822617-65822636 Msa0134280:exon 15.0%
!!! TTTATTTAATTTCAAGTACA+TGG + chr1_3:65823001-65823020 Msa0134280:intron 15.0%
!!! GAAATAATTTTACAATTCAT+TGG - chr1_3:65824927-65824946 None:intergenic 15.0%
!! AAATTTGATAAAACAGAGAT+TGG + chr1_3:65821985-65822004 Msa0134280:five_prime_UTR 20.0%
!! CAAAAATAAAAAATCAGAGA+CGG - chr1_3:65822182-65822201 None:intergenic 20.0%
!!! CATTAATAGAAGTATGATTT+TGG + chr1_3:65822616-65822635 Msa0134280:exon 20.0%
!! AATTGTTTAGTTAGAAAACA+AGG + chr1_3:65822890-65822909 Msa0134280:intron 20.0%
!! ATGTTGGAATTATAAGTAAT+CGG + chr1_3:65822931-65822950 Msa0134280:intron 20.0%
!!! ATGTTTTTTTGTCAAAAAAG+GGG - chr1_3:65823482-65823501 None:intergenic 20.0%
!!! CATGTTTTTTTGTCAAAAAA+GGG - chr1_3:65823483-65823502 None:intergenic 20.0%
!!! ACTTAAAGTATCTTCAATAT+TGG + chr1_3:65823642-65823661 Msa0134280:CDS 20.0%
!!! ATGTTTTACAATTCTACTTA+AGG + chr1_3:65823741-65823760 Msa0134280:intron 20.0%
!!! TGTTTTACAATTCTACTTAA+GGG + chr1_3:65823742-65823761 Msa0134280:intron 20.0%
!!! AGAAATCATGATTTTGTAAA+AGG - chr1_3:65824042-65824061 None:intergenic 20.0%
!!! ATTACTTGCATTTTAAGTTT+AGG + chr1_3:65824368-65824387 Msa0134280:intron 20.0%
!! GAGTTGAAGTAATAATAATA+GGG + chr1_3:65824778-65824797 Msa0134280:three_prime_UTR 20.0%
! CATGTTAGAAAAATTGTTAG+TGG + chr1_3:65821734-65821753 Msa0134280:five_prime_UTR 25.0%
! TGGTAAGCCTAAAAAATTAA+AGG + chr1_3:65822005-65822024 Msa0134280:five_prime_UTR 25.0%
! AGATATGCCAATTATAACAA+AGG - chr1_3:65822349-65822368 None:intergenic 25.0%
!! TTTGTTATAATTGGCATATC+TGG + chr1_3:65822348-65822367 Msa0134280:five_prime_UTR 25.0%
! ATATATGGTGCAAAAAGATA+AGG + chr1_3:65822389-65822408 Msa0134280:five_prime_UTR 25.0%
! AAAATGAAATTGATCAAGAC+AGG - chr1_3:65822595-65822614 None:intergenic 25.0%
! ATAAAATGAAAACCATACAG+TGG - chr1_3:65822789-65822808 None:intergenic 25.0%
!!! AGATTATTCTAGAGTAGATT+AGG + chr1_3:65822969-65822988 Msa0134280:intron 25.0%
! AGAAATTACAATGTGTTTCA+AGG - chr1_3:65823441-65823460 None:intergenic 25.0%
! ATTGTAATTTCTCTTAATCC+AGG + chr1_3:65823449-65823468 Msa0134280:intron 25.0%
!!! CCATGTTTTTTTGTCAAAAA+AGG - chr1_3:65823484-65823503 None:intergenic 25.0%
!!! CCTTTTTTGACAAAAAAACA+TGG + chr1_3:65823481-65823500 Msa0134280:CDS 25.0%
! GCACTGAATTATGTTAAATA+GGG + chr1_3:65823764-65823783 Msa0134280:intron 25.0%
!! ACTCTATAAGAATTTTACAC+AGG + chr1_3:65823789-65823808 Msa0134280:intron 25.0%
!! CTCTATAAGAATTTTACACA+GGG + chr1_3:65823790-65823809 Msa0134280:intron 25.0%
! TAGAATAAAAATGACAGAAC+TGG - chr1_3:65823857-65823876 None:intergenic 25.0%
! GATATCACTATTTACCTTAT+TGG - chr1_3:65824012-65824031 None:intergenic 25.0%
! AGTACTATAATATGAAATCC+AGG - chr1_3:65824065-65824084 None:intergenic 25.0%
! AACTTAAAATGCAAGTAATC+TGG - chr1_3:65824367-65824386 None:intergenic 25.0%
! ACCAAAAATTTATCTGTTGT+AGG + chr1_3:65824420-65824439 Msa0134280:intron 25.0%
!! TCCTACAACAGATAAATTTT+TGG - chr1_3:65824424-65824443 None:intergenic 25.0%
! GGAGTTGAAGTAATAATAAT+AGG + chr1_3:65824777-65824796 Msa0134280:three_prime_UTR 25.0%
!! TCTGGATATGTATTTTCAAT+TGG - chr1_3:65824880-65824899 None:intergenic 25.0%
! ATTGAAAATACATATCCAGA+CGG + chr1_3:65824880-65824899 Msa0134280:three_prime_UTR 25.0%
! TTGATGTTACAAATGATGAA+AGG + chr1_3:65825001-65825020 Msa0134280:three_prime_UTR 25.0%
! TATTTAAATGGTACAAGCTT+TGG + chr1_3:65825034-65825053 Msa0134280:three_prime_UTR 25.0%
AAAAATTGTTAGTGGTTGTG+AGG + chr1_3:65821742-65821761 Msa0134280:five_prime_UTR 30.0%
!!! GCTCTCACCTTTAATTTTTT+AGG - chr1_3:65822015-65822034 None:intergenic 30.0%
!! TCTCAAAGTTTTGTACAAGA+AGG - chr1_3:65822230-65822249 None:intergenic 30.0%
!! TTCTAGTTTCTTATTCTCCT+CGG + chr1_3:65822258-65822277 Msa0134280:five_prime_UTR 30.0%
ACAAAGGTCGATCATAAAAA+AGG - chr1_3:65822333-65822352 None:intergenic 30.0%
! TGATCGACCTTTGTTATAAT+TGG + chr1_3:65822339-65822358 Msa0134280:five_prime_UTR 30.0%
GGTTATAACCAATTTATGCA+GGG - chr1_3:65822494-65822513 None:intergenic 30.0%
TGGTTATAACCAATTTATGC+AGG - chr1_3:65822495-65822514 None:intergenic 30.0%
GAATGGAACACAAATACTAT+TGG - chr1_3:65822515-65822534 None:intergenic 30.0%
!!! AGTATGATTTTGGGATTTGT+TGG + chr1_3:65822626-65822645 Msa0134280:exon 30.0%
TAGGCCAAAGAATATATACA+AGG + chr1_3:65822690-65822709 Msa0134280:CDS 30.0%
GGATCCTTGTATATATTCTT+TGG - chr1_3:65822697-65822716 None:intergenic 30.0%
CAAGGAATAAGTTGTGAAAA+AGG + chr1_3:65822908-65822927 Msa0134280:intron 30.0%
TAAGTTGTGAAAAAGGATGT+TGG + chr1_3:65822915-65822934 Msa0134280:intron 30.0%
TGAGAGAATATGATAGATGA+AGG - chr1_3:65823136-65823155 None:intergenic 30.0%
TCCTCAATACATCTATTAAG+CGG - chr1_3:65823408-65823427 None:intergenic 30.0%
TTACAATGTGTTTCAAGGAA+AGG - chr1_3:65823436-65823455 None:intergenic 30.0%
! GTTACTCTTTTGAACAGATT+TGG + chr1_3:65823580-65823599 Msa0134280:intron 30.0%
! GGCACTGAATTATGTTAAAT+AGG + chr1_3:65823763-65823782 Msa0134280:intron 30.0%
!!! GCTCATTTTTTATGAATGCT+AGG + chr1_3:65823916-65823935 Msa0134280:intron 30.0%
TCACTATTTACCTTATTGGT+AGG - chr1_3:65824008-65824027 None:intergenic 30.0%
TACAAAATCATGATTTCTCC+TGG + chr1_3:65824044-65824063 Msa0134280:intron 30.0%
! TACAACAAAATTTGTTCTCG+AGG - chr1_3:65824128-65824147 None:intergenic 30.0%
ATGTAATGTCTGTCACTAAT+AGG + chr1_3:65824299-65824318 Msa0134280:intron 30.0%
! TAATAGGTTCTTTGTTTGCT+TGG + chr1_3:65824315-65824334 Msa0134280:intron 30.0%
AGAAAGTGAGTGCTTTATAT+TGG + chr1_3:65824724-65824743 Msa0134280:exon 30.0%
!! TTCTGTGACCTATTTTAAGT+TGG + chr1_3:65824843-65824862 Msa0134280:three_prime_UTR 30.0%
!!! TCTGTGACCTATTTTAAGTT+GGG + chr1_3:65824844-65824863 Msa0134280:three_prime_UTR 30.0%
GGAGTATGTCTGTATTTAAA+TGG + chr1_3:65825022-65825041 Msa0134280:three_prime_UTR 30.0%
AGGGAAAACATTCATCACAA+AGG - chr1_3:65821773-65821792 None:intergenic 35.0%
TTGATCATGACTAATGAGTG+GGG - chr1_3:65821808-65821827 None:intergenic 35.0%
GTTGATCATGACTAATGAGT+GGG - chr1_3:65821809-65821828 None:intergenic 35.0%
AAGGTAATTGACAGGAAAAG+AGG - chr1_3:65821889-65821908 None:intergenic 35.0%
TAGAAACAAATGGACGAAAC+AGG - chr1_3:65821912-65821931 None:intergenic 35.0%
!! GTTTCGTCCATTTGTTTCTA+AGG + chr1_3:65821912-65821931 Msa0134280:five_prime_UTR 35.0%
GAGTATGAAATCTACAGGAA+GGG - chr1_3:65821941-65821960 None:intergenic 35.0%
AGAGTATGAAATCTACAGGA+AGG - chr1_3:65821942-65821961 None:intergenic 35.0%
ATTTCATACTCTCCCAAGAT+TGG + chr1_3:65821950-65821969 Msa0134280:five_prime_UTR 35.0%
TTTCATACTCTCCCAAGATT+GGG + chr1_3:65821951-65821970 Msa0134280:five_prime_UTR 35.0%
ATTAAAGGTGAGAGCTAAAC+AGG + chr1_3:65822020-65822039 Msa0134280:five_prime_UTR 35.0%
AAGGATGAACGATGTATCAA+GGG - chr1_3:65822211-65822230 None:intergenic 35.0%
TTTCTTATTCTCCTCGGATT+GGG + chr1_3:65822264-65822283 Msa0134280:five_prime_UTR 35.0%
AAAAGAGCAGACTGATCAAA+GGG - chr1_3:65822307-65822326 None:intergenic 35.0%
TATAACCAATTTATGCAGGG+TGG - chr1_3:65822491-65822510 None:intergenic 35.0%
ATATACAAGGATCCAGATGA+TGG + chr1_3:65822703-65822722 Msa0134280:CDS 35.0%
TATACAAGGATCCAGATGAT+GGG + chr1_3:65822704-65822723 Msa0134280:CDS 35.0%
TTAAGCAATAGCTGCATATG+TGG + chr1_3:65822836-65822855 Msa0134280:intron 35.0%
TGTCGAGTCATGTATTTGAA+AGG + chr1_3:65822860-65822879 Msa0134280:intron 35.0%
GTCGAGTCATGTATTTGAAA+GGG + chr1_3:65822861-65822880 Msa0134280:intron 35.0%
! TCTAGAGTAGATTAGGTGTT+TGG + chr1_3:65822976-65822995 Msa0134280:intron 35.0%
CAAGTACATGGTCTGTTTAA+CGG + chr1_3:65823013-65823032 Msa0134280:intron 35.0%
AAGTACATGGTCTGTTTAAC+GGG + chr1_3:65823014-65823033 Msa0134280:intron 35.0%
GACATTGAGTTTAGTGACAT+TGG + chr1_3:65823049-65823068 Msa0134280:intron 35.0%
! ACATTGAGCTTTTGTGACAT+CGG + chr1_3:65823217-65823236 Msa0134280:intron 35.0%
TCCGCTTAATAGATGTATTG+AGG + chr1_3:65823404-65823423 Msa0134280:intron 35.0%
ATGCTTCGTCCTCAAAATAT+CGG - chr1_3:65823613-65823632 None:intergenic 35.0%
! TAGCGATGCTTTGTTCAAAT+GGG + chr1_3:65823875-65823894 Msa0134280:intron 35.0%
GAAGAAGCTCAAGAAAATAG+AGG - chr1_3:65823953-65823972 None:intergenic 35.0%
! TGCAAATTTTCGCAATGCAA+TGG + chr1_3:65823977-65823996 Msa0134280:CDS 35.0%
TTGAGACAGATAGACAGATA+TGG + chr1_3:65824170-65824189 Msa0134280:intron 35.0%
TACCTACTGCATCACAATTT+CGG - chr1_3:65824236-65824255 None:intergenic 35.0%
TTTCTTTGCCTTGTGTTTCT+TGG + chr1_3:65824393-65824412 Msa0134280:intron 35.0%
! TCTACTTCTGGAAGAACTAT+AGG + chr1_3:65824464-65824483 Msa0134280:CDS 35.0%
GGAAGAACTATAGGAACAAT+CGG + chr1_3:65824473-65824492 Msa0134280:CDS 35.0%
!!! CATGTCATTTTTTGCACTAC+CGG - chr1_3:65824677-65824696 None:intergenic 35.0%
GAAATACTTCAGCTGATAGA+AGG + chr1_3:65824698-65824717 Msa0134280:CDS 35.0%
TGTGCTACCCAACTTAAAAT+AGG - chr1_3:65824854-65824873 None:intergenic 35.0%
GGTTGATCATGACTAATGAG+TGG - chr1_3:65821810-65821829 None:intergenic 40.0%
ACAGGAAAAGAGGCTAATGA+TGG - chr1_3:65821879-65821898 None:intergenic 40.0%
GAAACAGGAAGGTAATTGAC+AGG - chr1_3:65821897-65821916 None:intergenic 40.0%
AACAAATGGACGAAACAGGA+AGG - chr1_3:65821908-65821927 None:intergenic 40.0%
AGGGAAGCCTTAGAAACAAA+TGG - chr1_3:65821922-65821941 None:intergenic 40.0%
! TGGGAGAGTATGAAATCTAC+AGG - chr1_3:65821946-65821965 None:intergenic 40.0%
GTATCAAAGCTCCCAATCTT+GGG - chr1_3:65821965-65821984 None:intergenic 40.0%
TGTATCAAAGCTCCCAATCT+TGG - chr1_3:65821966-65821985 None:intergenic 40.0%
AAACAGGAAAGGTGGAATGA+CGG + chr1_3:65822036-65822055 Msa0134280:five_prime_UTR 40.0%
ATCTATTGTGCAGCACTTGT+TGG - chr1_3:65822085-65822104 None:intergenic 40.0%
AGAAGGGACATATGCCAATT+GGG - chr1_3:65822134-65822153 None:intergenic 40.0%
TTAGAGGAGGAATGACAGAA+GGG - chr1_3:65822150-65822169 None:intergenic 40.0%
ACGGATAAAACAGTTAGAGG+AGG - chr1_3:65822163-65822182 None:intergenic 40.0%
GAGACGGATAAAACAGTTAG+AGG - chr1_3:65822166-65822185 None:intergenic 40.0%
AGGATGAACGATGTATCAAG+GGG - chr1_3:65822210-65822229 None:intergenic 40.0%
GAAGGATGAACGATGTATCA+AGG - chr1_3:65822212-65822231 None:intergenic 40.0%
GTTTCTTATTCTCCTCGGAT+TGG + chr1_3:65822263-65822282 Msa0134280:five_prime_UTR 40.0%
CAAAAGAGCAGACTGATCAA+AGG - chr1_3:65822308-65822327 None:intergenic 40.0%
TGGTGCAAAAAGATAAGGAG+AGG + chr1_3:65822394-65822413 Msa0134280:five_prime_UTR 40.0%
! CTTCCTTTGAAAAACCAACC+AGG - chr1_3:65822460-65822479 None:intergenic 40.0%
ATACGGAAAGCCCTTGAAAA+GGG - chr1_3:65822553-65822572 None:intergenic 40.0%
TATACGGAAAGCCCTTGAAA+AGG - chr1_3:65822554-65822573 None:intergenic 40.0%
!!! GCTGATGGTTGTTTTTCTCT+AGG + chr1_3:65822671-65822690 Msa0134280:CDS 40.0%
GAAAACCATACAGTGGATGT+AGG - chr1_3:65822782-65822801 None:intergenic 40.0%
TAAAGTCACCGATGTCACAA+AGG + chr1_3:65823167-65823186 Msa0134280:intron 40.0%
ACATTGAGCCTTTGTGACAT+CGG - chr1_3:65823178-65823197 None:intergenic 40.0%
TCAATGTCACGGAAACTACT+TGG + chr1_3:65823191-65823210 Msa0134280:intron 40.0%
CAATGTCACGGAAACTACTT+GGG + chr1_3:65823192-65823211 Msa0134280:intron 40.0%
CAAAAAAGGGGAATTTCACC+TGG - chr1_3:65823470-65823489 None:intergenic 40.0%
! TTTGAGGACGAAGCATTCAT+TGG + chr1_3:65823617-65823636 Msa0134280:CDS 40.0%
! TTGAGGACGAAGCATTCATT+GGG + chr1_3:65823618-65823637 Msa0134280:CDS 40.0%
CTTATTGTCCCACTAAAGTC+TGG + chr1_3:65823708-65823727 Msa0134280:intron 40.0%
! CTAGCGATGCTTTGTTCAAA+TGG + chr1_3:65823874-65823893 Msa0134280:intron 40.0%
TCAAGAAAATAGAGGCAGTG+AGG - chr1_3:65823945-65823964 None:intergenic 40.0%
AATGGCACATCCTACCAATA+AGG + chr1_3:65823995-65824014 Msa0134280:CDS 40.0%
TACCGAAATTGTGATGCAGT+AGG + chr1_3:65824231-65824250 Msa0134280:intron 40.0%
TGGTCAATCCAAGAAACACA+AGG - chr1_3:65824404-65824423 None:intergenic 40.0%
ATAGACAGCAGTTCTACTTC+TGG + chr1_3:65824452-65824471 Msa0134280:CDS 40.0%
ATACTTTCTTCCATCGCAGA+TGG + chr1_3:65824755-65824774 Msa0134280:three_prime_UTR 40.0%
TACTTTCTTCCATCGCAGAT+GGG + chr1_3:65824756-65824775 Msa0134280:three_prime_UTR 40.0%
AACCCTTGTATGACAAGCCA+CGG + chr1_3:65821826-65821845 Msa0134280:five_prime_UTR 45.0%
AGGTGAGAGCTAAACAGGAA+AGG + chr1_3:65822025-65822044 Msa0134280:five_prime_UTR 45.0%
TGAGAGCTAAACAGGAAAGG+TGG + chr1_3:65822028-65822047 Msa0134280:five_prime_UTR 45.0%
GAAGGGACATATGCCAATTG+GGG - chr1_3:65822133-65822152 None:intergenic 45.0%
CAGAAGGGACATATGCCAAT+TGG - chr1_3:65822135-65822154 None:intergenic 45.0%
GTTAGAGGAGGAATGACAGA+AGG - chr1_3:65822151-65822170 None:intergenic 45.0%
!! TAAGGAGAGGCACTTTATGC+TGG + chr1_3:65822407-65822426 Msa0134280:five_prime_UTR 45.0%
GTTATGAGTGTAAGCCAACC+AGG + chr1_3:65822434-65822453 Msa0134280:five_prime_UTR 45.0%
TTTGAAAAACCAACCAGGCC+TGG - chr1_3:65822455-65822474 None:intergenic 45.0%
!!! AGGCCTGGTTGGTTTTTCAA+AGG + chr1_3:65822454-65822473 Msa0134280:five_prime_UTR 45.0%
TCAAGCCACCCTGCATAAAT+TGG + chr1_3:65822483-65822502 Msa0134280:five_prime_UTR 45.0%
! TGAAGTGTGTGCCCTTTTCA+AGG + chr1_3:65822539-65822558 Msa0134280:five_prime_UTR 45.0%
! GAAGTGTGTGCCCTTTTCAA+GGG + chr1_3:65822540-65822559 Msa0134280:five_prime_UTR 45.0%
! ACAACGGTTTTTGCTGGAAC+TGG + chr1_3:65822729-65822748 Msa0134280:CDS 45.0%
! GGATGTAGGTAGGATTAGCA+AGG - chr1_3:65822768-65822787 None:intergenic 45.0%
TCCTACCTACATCCACTGTA+TGG + chr1_3:65822774-65822793 Msa0134280:intron 45.0%
ACCATACAGTGGATGTAGGT+AGG - chr1_3:65822778-65822797 None:intergenic 45.0%
!! TCTGTTTAACGGGTTGCACT+TGG + chr1_3:65823024-65823043 Msa0134280:intron 45.0%
GTCACAAAGGCTCAATGTCA+CGG + chr1_3:65823180-65823199 Msa0134280:intron 45.0%
TGTGACATCGGTGACTTTAG+TGG + chr1_3:65823229-65823248 Msa0134280:intron 45.0%
GAGTTGCACTTGGATGACAT+TGG - chr1_3:65823339-65823358 None:intergenic 45.0%
GGAAACTACTTGGGTGACAT+TGG - chr1_3:65823201-65823220 None:intergenic 45.0%
ACCCAAGTAGTTTCCCTGTT+GGG + chr1_3:65823366-65823385 Msa0134280:intron 45.0%
ACCCAACAGGGAAACTACTT+GGG - chr1_3:65823370-65823389 None:intergenic 45.0%
AACCCAACAGGGAAACTACT+TGG - chr1_3:65823371-65823390 None:intergenic 45.0%
CATGCTTTGTCATTGCTGCT+TGG + chr1_3:65823504-65823523 Msa0134280:CDS 45.0%
! GGCTCAGAACCGATATTTTG+AGG + chr1_3:65823601-65823620 Msa0134280:CDS 45.0%
TCCCACTAAAGTCTGGAAGA+GGG + chr1_3:65823715-65823734 Msa0134280:intron 45.0%
TCCCTCTTCCAGACTTTAGT+GGG - chr1_3:65823719-65823738 None:intergenic 45.0%
ATCCCTCTTCCAGACTTTAG+TGG - chr1_3:65823720-65823739 None:intergenic 45.0%
! CGATGCTTTGTTCAAATGGG+TGG + chr1_3:65823878-65823897 Msa0134280:intron 45.0%
! ATACCGTATGGCATTGGAGA+AGG - chr1_3:65824643-65824662 None:intergenic 45.0%
TCTAACGAGTTTCAACCGTC+TGG - chr1_3:65824898-65824917 None:intergenic 45.0%
TAATGAGTGGGGAGTCACAG+AGG - chr1_3:65821797-65821816 None:intergenic 50.0%
! AGCCGTGGCTTGTCATACAA+GGG - chr1_3:65821831-65821850 None:intergenic 50.0%
TTATCGCTCCACTCCCCAAT+TGG + chr1_3:65822117-65822136 Msa0134280:five_prime_UTR 50.0%
GACATATGCCAATTGGGGAG+TGG - chr1_3:65822128-65822147 None:intergenic 50.0%
TGCTGTCTAAACCCAATCCG+AGG - chr1_3:65822278-65822297 None:intergenic 50.0%
AAAAACCAACCAGGCCTGGT+TGG - chr1_3:65822451-65822470 None:intergenic 50.0%
GCGCTTAAGTACAGCTCAAC+AGG - chr1_3:65822653-65822672 None:intergenic 50.0%
!! GAGCTGTACTTAAGCGCTGA+TGG + chr1_3:65822656-65822675 Msa0134280:CDS 50.0%
!! ATCCAGATGATGGGCGACAA+CGG + chr1_3:65822713-65822732 Msa0134280:CDS 50.0%
GGTGACATTGGAGTTGCACT+TGG - chr1_3:65823349-65823368 None:intergenic 50.0%
CACCCAAGTAGTTTCCCTGT+TGG + chr1_3:65823365-65823384 Msa0134280:intron 50.0%
GTCCCACTAAAGTCTGGAAG+AGG + chr1_3:65823714-65823733 Msa0134280:intron 50.0%
!! CTAGGTTCTGGAAGTGGTCT+TGG - chr1_3:65824514-65824533 None:intergenic 50.0%
!! ACACTTGTAGCAGGTACTGG+TGG - chr1_3:65824592-65824611 None:intergenic 50.0%
GCAACACTTGTAGCAGGTAC+TGG - chr1_3:65824595-65824614 None:intergenic 50.0%
GTCACAGCAACACTTGTAGC+AGG - chr1_3:65824601-65824620 None:intergenic 50.0%
GCTCCTTCTCCAATGCCATA+CGG + chr1_3:65824637-65824656 Msa0134280:CDS 50.0%
! TTTTTGCACTACCGGAGCCA+GGG - chr1_3:65824669-65824688 None:intergenic 50.0%
!!! TTTTTTGCACTACCGGAGCC+AGG - chr1_3:65824670-65824689 None:intergenic 50.0%
ACTTCAACTCCCATCTGCGA+TGG - chr1_3:65824768-65824787 None:intergenic 50.0%
AGTGGGGAGTCACAGAGGAA+GGG - chr1_3:65821792-65821811 None:intergenic 55.0%
! CAGCCGTGGCTTGTCATACA+AGG - chr1_3:65821832-65821851 None:intergenic 55.0%
GGCACACACTTCACGCTGAA+TGG - chr1_3:65822532-65822551 None:intergenic 55.0%
AACCGTTGTCGCCCATCATC+TGG - chr1_3:65822718-65822737 None:intergenic 55.0%
! TGGGCGACAACGGTTTTTGC+TGG + chr1_3:65822723-65822742 Msa0134280:CDS 55.0%
CCTCTCAGTGCAACCCAACA+GGG - chr1_3:65823382-65823401 None:intergenic 55.0%
! CCTGTTGGGTTGCACTGAGA+GGG + chr1_3:65823380-65823399 Msa0134280:intron 55.0%
!! GCAGCACTAGGTTCTGGAAG+TGG - chr1_3:65824520-65824539 None:intergenic 55.0%
TAGTGCATGCACAGGTGGCT+GGG - chr1_3:65824569-65824588 None:intergenic 55.0%
! ACTGGTGGTAGTGCATGCAC+AGG - chr1_3:65824577-65824596 None:intergenic 55.0%
! GGGGGTATACCGTATGGCAT+TGG - chr1_3:65824649-65824668 None:intergenic 55.0%
ATGCCATACGGTATACCCCC+TGG + chr1_3:65824649-65824668 Msa0134280:CDS 55.0%
! TTTTGCACTACCGGAGCCAG+GGG - chr1_3:65824668-65824687 None:intergenic 55.0%
GAGTGGGGAGTCACAGAGGA+AGG - chr1_3:65821793-65821812 None:intergenic 60.0%
GAGTGTAAGCCAACCAGGCC+TGG + chr1_3:65822439-65822458 Msa0134280:five_prime_UTR 60.0%
GTAAGCCAACCAGGCCTGGT+TGG + chr1_3:65822443-65822462 Msa0134280:five_prime_UTR 60.0%
! CCCTGTTGGGTTGCACTGAG+AGG + chr1_3:65823379-65823398 Msa0134280:intron 60.0%
CCCTCTCAGTGCAACCCAAC+AGG - chr1_3:65823383-65823402 None:intergenic 60.0%
! GGAAGCGCAGCACTAGGTTC+TGG - chr1_3:65824526-65824545 None:intergenic 60.0%
GAACCTAGTGCTGCGCTTCC+TGG + chr1_3:65824526-65824545 Msa0134280:CDS 60.0%
TAGTGCTGCGCTTCCTGGTC+CGG + chr1_3:65824531-65824550 Msa0134280:CDS 60.0%
AGTGCATGCACAGGTGGCTG+GGG - chr1_3:65824568-65824587 None:intergenic 60.0%
GTAGTGCATGCACAGGTGGC+TGG - chr1_3:65824570-65824589 None:intergenic 60.0%
! GGTGGTAGTGCATGCACAGG+TGG - chr1_3:65824574-65824593 None:intergenic 60.0%
! GAGCCAGGGGGTATACCGTA+TGG - chr1_3:65824655-65824674 None:intergenic 60.0%
TACGGTATACCCCCTGGCTC+CGG + chr1_3:65824655-65824674 Msa0134280:CDS 60.0%
TTTGCACTACCGGAGCCAGG+GGG - chr1_3:65824667-65824686 None:intergenic 60.0%
GCTTGCAGTGAGAGCAGCCG+TGG - chr1_3:65821846-65821865 None:intergenic 65.0%
! GGACCAGGAAGCGCAGCACT+AGG - chr1_3:65824532-65824551 None:intergenic 65.0%
AGGTGGCTGGGGTTGAGTCG+AGG - chr1_3:65824557-65824576 None:intergenic 65.0%
GGTTGAGTCGAGGCCGGACC+AGG - chr1_3:65824547-65824566 None:intergenic 70.0%
GGCTGGGGTTGAGTCGAGGC+CGG - chr1_3:65824553-65824572 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 65821714 65825064 65821714 ID=Msa0134280;Name=Msa0134280
chr1_3 mRNA 65821714 65825064 65821714 ID=Msa0134280-mRNA-1;Parent=Msa0134280;Name=Msa0134280-mRNA-1;_AED=0.30;_eAED=0.30;_QI=915|0.5|0.8|0.8|0.5|0.8|5|332|227
chr1_3 exon 65821714 65822790 65821714 ID=Msa0134280-mRNA-1:exon:22387;Parent=Msa0134280-mRNA-1
chr1_3 exon 65823471 65823525 65823471 ID=Msa0134280-mRNA-1:exon:22388;Parent=Msa0134280-mRNA-1
chr1_3 exon 65823597 65823693 65823597 ID=Msa0134280-mRNA-1:exon:22389;Parent=Msa0134280-mRNA-1
chr1_3 exon 65823938 65824016 65823938 ID=Msa0134280-mRNA-1:exon:22390;Parent=Msa0134280-mRNA-1
chr1_3 exon 65824442 65825064 65824442 ID=Msa0134280-mRNA-1:exon:22391;Parent=Msa0134280-mRNA-1
chr1_3 five_prime_UTR 65821714 65822628 65821714 ID=Msa0134280-mRNA-1:five_prime_utr;Parent=Msa0134280-mRNA-1
chr1_3 CDS 65822629 65822790 65822629 ID=Msa0134280-mRNA-1:cds;Parent=Msa0134280-mRNA-1
chr1_3 CDS 65823471 65823525 65823471 ID=Msa0134280-mRNA-1:cds;Parent=Msa0134280-mRNA-1
chr1_3 CDS 65823597 65823693 65823597 ID=Msa0134280-mRNA-1:cds;Parent=Msa0134280-mRNA-1
chr1_3 CDS 65823938 65824016 65823938 ID=Msa0134280-mRNA-1:cds;Parent=Msa0134280-mRNA-1
chr1_3 CDS 65824442 65824732 65824442 ID=Msa0134280-mRNA-1:cds;Parent=Msa0134280-mRNA-1
chr1_3 three_prime_UTR 65824733 65825064 65824733 ID=Msa0134280-mRNA-1:three_prime_utr;Parent=Msa0134280-mRNA-1
Gene Sequence

>Msa0134280

ATGATTTTGGGATTTGTTGGACCTGTTGAGCTGTACTTAAGCGCTGATGGTTGTTTTTCTCTAGGCCAAAGAATATATACAAGGATCCAGATGATGGGCGACAACGGTTTTTGCTGGAACTGGAATTTGTTCAATGCCTTGCTAATCCTACCTACATCCACTGTGAAATTCCCCTTTTTTGACAAAAAAACATGGCATGCTTTGTCATTGCTGCTTGATTTGGCTCAGAACCGATATTTTGAGGACGAAGCATTCATTGGGTACTTAAAGTATCTTCAATATTGGCAACGCCCTGAATATATTAAATTTATCATGTATCCTCACTGCCTCTATTTTCTTGAGCTTCTTCAGAATGCAAATTTTCGCAATGCAATGGCACATCCTACCAATAAGGAATTGACACATAGACAGCAGTTCTACTTCTGGAAGAACTATAGGAACAATCGGCTAAAACACATTTTACCAAGACCACTTCCAGAACCTAGTGCTGCGCTTCCTGGTCCGGCCTCGACTCAACCCCAGCCACCTGTGCATGCACTACCACCAGTACCTGCTACAAGTGTTGCTGTGACAACATCGTCTAGTCAAGCTCCTTCTCCAATGCCATACGGTATACCCCCTGGCTCCGGTAGTGCAAAAAATGACATGAGAAATACTTCAGCTGATAGAAGGAAAAGAAAGTGA
Protein sequence

>Msa0134280

MILGFVGPVELYLSADGCFSLGQRIYTRIQMMGDNGFCWNWNLFNALLILPTSTVKFPFFDKKTWHALSLLLDLAQNRYFEDEAFIGYLKYLQYWQRPEYIKFIMYPHCLYFLELLQNANFRNAMAHPTNKELTHRQQFYFWKNYRNNRLKHILPRPLPEPSAALPGPASTQPQPPVHALPPVPATSVAVTTSSSQAPSPMPYGIPPGSGSAKNDMRNTSADRRKRK*