AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0092240

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 85 sgRNAs with CRISPR-Local

Find 269 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACTATAGTCGGAGGGCTTT+TGG 0.140122 1_2:-74639875 Msa0092240:CDS
CACTTCCAACTAATATTTAA+TGG 0.186346 1_2:+74639743 None:intergenic
GACTATAGTTGGAGAGCTTT+TGG 0.218783 1_2:-74639960 Msa0092240:intron
TAGTTACAGAAGGTTATTTA+TGG 0.222425 1_2:-74639538 Msa0092240:three_prime_UTR
TAAATAACCTTCTGTAACTA+TGG 0.262813 1_2:+74639541 None:intergenic
GTAGTAGCTGTAGAACTATT+TGG 0.267786 1_2:+74640264 None:intergenic
AACCTGGAATTTCACGATTC+TGG 0.271733 1_2:+74642913 None:intergenic
ACCTGGATAACAAAGAAATT+TGG 0.288436 1_2:+74639648 None:intergenic
TAGTAGCTGTAGAACTATTT+GGG 0.317347 1_2:+74640265 None:intergenic
TGTATTTAATTCAGGCAATC+TGG 0.329922 1_2:-74640948 Msa0092240:intron
TCTGCAAAGGCAAAACCTTT+TGG 0.329956 1_2:+74640830 None:intergenic
CACTTAATGACTTGACTGAT+TGG 0.343257 1_2:-74639487 Msa0092240:three_prime_UTR
TGCATCAGGGCGACGTGGCC+TGG 0.360741 1_2:+74639826 None:intergenic
TCTGCTATTAAACAGATATC+TGG 0.363071 1_2:-74640303 Msa0092240:five_prime_UTR
GCTTAAAGCACCAGTAGATA+TGG 0.369400 1_2:+74639694 None:intergenic
TGGATAACGCTAGCCGTTCC+AGG 0.375993 1_2:-74639844 Msa0092240:CDS
GAGATTGGGCTAACTGCATC+AGG 0.387744 1_2:+74639812 None:intergenic
AGAACTTCAACTTGTTTACT+TGG 0.393583 1_2:+74643006 None:intergenic
TCACTTTGGTGGCAACCATT+TGG 0.393886 1_2:-74639897 Msa0092240:CDS
ACCAAATTTCTTTGTTATCC+AGG 0.397630 1_2:-74639649 Msa0092240:three_prime_UTR
TTTACTTGGTCTTGTGGGAA+TGG 0.401503 1_2:+74643020 None:intergenic
CGCCAGAATCGTGAAATTCC+AGG 0.411538 1_2:-74642915 Msa0092240:intron
ACTTGTTTACTTGGTCTTGT+GGG 0.415205 1_2:+74643015 None:intergenic
CAAGGAAGGAAATGATAAAC+AGG 0.418150 1_2:+74639608 None:intergenic
AAGAACGCACCGCAAACTTC+AGG 0.422993 1_2:+74640735 None:intergenic
CTATTTATTTGCACAGGGTA+AGG 0.429684 1_2:+74640210 None:intergenic
AGCTTCCATTAAATATTAGT+TGG 0.433482 1_2:-74639748 Msa0092240:exon
TAGTAAGGTGGGTAAGAGAT+TGG 0.436729 1_2:+74639797 None:intergenic
TCTGAACTATTTATTTGCAC+AGG 0.438610 1_2:+74640204 None:intergenic
ATTTGCACAGGGTAAGGCCT+TGG 0.443958 1_2:+74640216 None:intergenic
AACTTGTTTACTTGGTCTTG+TGG 0.450838 1_2:+74643014 None:intergenic
ACCGACCAATCTAATGTGTA+TGG 0.465656 1_2:-74639923 Msa0092240:exon
CATTAGATTGGTCGGTTGCA+CGG 0.466300 1_2:+74639930 None:intergenic
CTTGCACATTCCTAAAGATA+AGG 0.486567 1_2:-74640864 Msa0092240:five_prime_UTR
TTCCTTCCTTGTTATTTGTG+AGG 0.487134 1_2:-74639596 Msa0092240:three_prime_UTR
CCCTCCGACTATAGTCCAAA+TGG 0.489019 1_2:+74639882 None:intergenic
ATTGAAAATATTAGTAAGGT+GGG 0.489596 1_2:+74639786 None:intergenic
GAGATCCATACACATTAGAT+TGG 0.489795 1_2:+74639918 None:intergenic
ATCTATTGAAAATATTAGTA+AGG 0.490677 1_2:+74639782 None:intergenic
TGGATGAGAGGGTGACAGAC+AGG 0.491893 1_2:-74640928 Msa0092240:five_prime_UTR
GCTAGATATTCCATATCTAC+TGG 0.492239 1_2:-74639704 Msa0092240:three_prime_UTR
AGTAAGGTGGGTAAGAGATT+GGG 0.492624 1_2:+74639798 None:intergenic
AAGTTCTACTGACTCAACTT+TGG 0.510618 1_2:-74642990 Msa0092240:five_prime_UTR
TATGATATATTAATTCAAGC+TGG 0.514163 1_2:-74640899 Msa0092240:five_prime_UTR
TCCATACACATTAGATTGGT+CGG 0.515520 1_2:+74639922 None:intergenic
TTCGTTTGTACAGTGTTACA+AGG 0.518910 1_2:+74640759 None:intergenic
CGTGCGTTAAATTATTCTCA+AGG 0.530851 1_2:-74640132 Msa0092240:intron
CAAACGAACCCTGAAGTTTG+CGG 0.533929 1_2:-74640744 Msa0092240:intron
AATTTCTTTGTTATCCAGGT+AGG 0.541561 1_2:-74639645 Msa0092240:three_prime_UTR
TATTGAAAATATTAGTAAGG+TGG 0.542457 1_2:+74639785 None:intergenic
ATACCTCCATGTACTTCTCT+AGG 0.542852 1_2:-74639572 Msa0092240:three_prime_UTR
CTCAGTTTCATATTCTGCAA+AGG 0.546183 1_2:+74640817 None:intergenic
CAGGGCGACGTGGCCTGGAA+CGG 0.550905 1_2:+74639831 None:intergenic
CTGAACTATTTATTTGCACA+GGG 0.556003 1_2:+74640205 None:intergenic
CAACAGAGCTACAACTTGCT+CGG 0.556504 1_2:+74639462 None:intergenic
TACATAAGCATCAACCTACC+TGG 0.562206 1_2:+74639631 None:intergenic
AATGTGTATGGATCTCACTT+TGG 0.572385 1_2:-74639911 Msa0092240:CDS
AGAACGCACCGCAAACTTCA+GGG 0.575012 1_2:+74640736 None:intergenic
TTAACGCACGATCTGAGAAT+CGG 0.575936 1_2:+74640145 None:intergenic
TTGGAAGTGAAAGTAGAATG+AGG 0.584938 1_2:-74639729 Msa0092240:three_prime_UTR
TAAAACTCCATAGTTACAGA+AGG 0.586880 1_2:-74639548 Msa0092240:three_prime_UTR
TTCAGGCAATCTGGATGAGA+GGG 0.588517 1_2:-74640939 Msa0092240:intron
TTCCTCACAAATAACAAGGA+AGG 0.592904 1_2:+74639594 None:intergenic
ATTCAGGCAATCTGGATGAG+AGG 0.594652 1_2:-74640940 Msa0092240:intron
CGGTCGGTGACAAACTTCGC+CGG 0.602665 1_2:+74642936 None:intergenic
TTGGTACACTACAACAACAA+TGG 0.605013 1_2:+74639667 None:intergenic
CTATTAAACAGATATCTGGA+AGG 0.608661 1_2:-74640299 Msa0092240:five_prime_UTR
GCAACCATTTGGACTATAGT+CGG 0.610307 1_2:-74639886 Msa0092240:CDS
GTGTATGGATCTCACTTTGG+TGG 0.612020 1_2:-74639908 Msa0092240:CDS
AATTTCACGATTCTGGCGGT+CGG 0.617900 1_2:+74642920 None:intergenic
CCATTTGGACTATAGTCGGA+GGG 0.622071 1_2:-74639882 Msa0092240:CDS
TATTTATGGCCTGTTTAAAG+CGG 0.628335 1_2:-74639524 Msa0092240:three_prime_UTR
CAAATTCTTCTCAACGACCA+AGG 0.631237 1_2:-74640233 Msa0092240:five_prime_UTR
CTAACTGCATCAGGGCGACG+TGG 0.635502 1_2:+74639821 None:intergenic
ACCATTTGGACTATAGTCGG+AGG 0.638568 1_2:-74639883 Msa0092240:CDS
CTGGAATTTCACGATTCTGG+CGG 0.648034 1_2:+74642916 None:intergenic
AGTAGCTGTAGAACTATTTG+GGG 0.648842 1_2:+74640266 None:intergenic
ATTAATTCAAGCTGGACGAG+TGG 0.649212 1_2:-74640891 Msa0092240:five_prime_UTR
TATTAAACAGATATCTGGAA+GGG 0.655175 1_2:-74640298 Msa0092240:five_prime_UTR
CTTTGCAGAATATGAAACTG+AGG 0.657421 1_2:-74640816 Msa0092240:five_prime_UTR
AGATTGGGCTAACTGCATCA+GGG 0.658192 1_2:+74639813 None:intergenic
GGTATTCCTCACAAATAACA+AGG 0.660496 1_2:+74639590 None:intergenic
TCGTTGAGAAGAATTTGACG+AGG 0.685367 1_2:+74640239 None:intergenic
GTCAGATATTGATTGCCTAG+CGG 0.717548 1_2:+74640167 None:intergenic
TAACCTAGAGAAGTACATGG+AGG 0.753420 1_2:+74639569 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATTTATATTTAGTTTAC+TGG - chr1_2:74640946-74640965 Msa0092240:intron 10.0%
!! TTGAAAAAAGAAAATAAGAA+AGG + chr1_2:74639570-74639589 None:intergenic 15.0%
!! ATTAATTATAAGATACAGTA+TGG - chr1_2:74640109-74640128 Msa0092240:intron 15.0%
!!! TATTTTTAGAAAAACTGATA+TGG - chr1_2:74640419-74640438 Msa0092240:intron 15.0%
!!! TATTTCTATTGAAACAATTA+CGG + chr1_2:74640602-74640621 None:intergenic 15.0%
!!! TAGAAATAAAATTTTATTCG+AGG - chr1_2:74640861-74640880 Msa0092240:five_prime_UTR 15.0%
!!! AGAAATAAAATTTTATTCGA+GGG - chr1_2:74640862-74640881 Msa0092240:five_prime_UTR 15.0%
!! TTTGTATATGAACAAATTTA+AGG - chr1_2:74641405-74641424 Msa0092240:intron 15.0%
!! TTGTATATGAACAAATTTAA+GGG - chr1_2:74641406-74641425 Msa0092240:intron 15.0%
!! ATCTATTGAAAATATTAGTA+AGG + chr1_2:74642677-74642696 None:intergenic 15.0%
!!! TAGAAATAACCTTTTATTAG+AGG - chr1_2:74640614-74640633 Msa0092240:intron 20.0%
!!! GAAATAAAATTTTATTCGAG+GGG - chr1_2:74640863-74640882 Msa0092240:five_prime_UTR 20.0%
!!! ATTTATATTTAGTTTACTGG+TGG - chr1_2:74640949-74640968 Msa0092240:intron 20.0%
!! TAAAGAGTAATATAATGTGA+GGG + chr1_2:74641083-74641102 None:intergenic 20.0%
!! TTAAAGAGTAATATAATGTG+AGG + chr1_2:74641084-74641103 None:intergenic 20.0%
!! TAAAGTTTCAATTCTTCATA+GGG - chr1_2:74641362-74641381 Msa0092240:intron 20.0%
!! TGTATATGAACAAATTTAAG+GGG - chr1_2:74641407-74641426 Msa0092240:intron 20.0%
!! TATGATATATTAATTCAAGC+TGG - chr1_2:74641557-74641576 Msa0092240:intron 20.0%
!!! TTGCATAAAATATTTTCAAC+AGG + chr1_2:74641884-74641903 None:intergenic 20.0%
!! TATTAAACGAAGTTAATAGT+TGG - chr1_2:74641907-74641926 Msa0092240:intron 20.0%
!! TTTAAATTTCTTTCATGTCA+TGG - chr1_2:74642107-74642126 Msa0092240:intron 20.0%
!! ATTGAAAATATTAGTAAGGT+GGG + chr1_2:74642673-74642692 None:intergenic 20.0%
!! TATTGAAAATATTAGTAAGG+TGG + chr1_2:74642674-74642693 None:intergenic 20.0%
!!! CAATTTTCAGTTAATTTGCA+AGG + chr1_2:74639941-74639960 None:intergenic 25.0%
! CTTGCAAATTAACTGAAAAT+TGG - chr1_2:74639939-74639958 Msa0092240:five_prime_UTR 25.0%
!! GATAATTTTCATATGCTATC+TGG + chr1_2:74640164-74640183 None:intergenic 25.0%
! TGAACATATTAAGAACTCTA+GGG - chr1_2:74640195-74640214 Msa0092240:five_prime_UTR 25.0%
! TTATCAAAAGCTCAAATGAT+TGG + chr1_2:74640337-74640356 None:intergenic 25.0%
! TCAATAGATCATTAACCAAT+TGG - chr1_2:74640708-74640727 Msa0092240:intron 25.0%
!! TCTTTTACAGAAGTAAACAT+AGG + chr1_2:74641191-74641210 None:intergenic 25.0%
! AGCAAGAAAAATATAGTTGT+GGG + chr1_2:74641332-74641351 None:intergenic 25.0%
! AAGCAAGAAAAATATAGTTG+TGG + chr1_2:74641333-74641352 None:intergenic 25.0%
! CTAAAGTTTCAATTCTTCAT+AGG - chr1_2:74641361-74641380 Msa0092240:intron 25.0%
! TGGTTACTTGTATTTAATTC+AGG - chr1_2:74641500-74641519 Msa0092240:intron 25.0%
!! TTTTCAGATTCCTTATCTTT+AGG + chr1_2:74641605-74641624 None:intergenic 25.0%
! GAAAGATTAAAAATTGCCAA+TGG + chr1_2:74641856-74641875 None:intergenic 25.0%
! AGCTGTATTTATTACATGAT+TGG + chr1_2:74642027-74642046 None:intergenic 25.0%
!! ATGTCATGGTTATTTGTTAA+TGG - chr1_2:74642121-74642140 Msa0092240:intron 25.0%
! TATTAAACAGATATCTGGAA+GGG - chr1_2:74642158-74642177 Msa0092240:intron 25.0%
! TTAATTCAATCTAATGCGTA+TGG - chr1_2:74642448-74642467 Msa0092240:intron 25.0%
! AGCTTCCATTAAATATTAGT+TGG - chr1_2:74642708-74642727 Msa0092240:intron 25.0%
! CACTTCCAACTAATATTTAA+TGG + chr1_2:74642716-74642735 None:intergenic 25.0%
! TAAATAACCTTCTGTAACTA+TGG + chr1_2:74642918-74642937 None:intergenic 25.0%
! TAGTTACAGAAGGTTATTTA+TGG - chr1_2:74642918-74642937 Msa0092240:five_prime_UTR 25.0%
! AGAACTTCAACTTGTTTACT+TGG + chr1_2:74639453-74639472 None:intergenic 30.0%
AGAAAATAAGAAAGGAAACC+TGG + chr1_2:74639562-74639581 None:intergenic 30.0%
TGTAGATCTATTTCGAAACA+GGG + chr1_2:74639803-74639822 None:intergenic 30.0%
ATGTAGATCTATTTCGAAAC+AGG + chr1_2:74639804-74639823 None:intergenic 30.0%
CCTCTAAATTGAAAAAAGTG+AGG + chr1_2:74639871-74639890 None:intergenic 30.0%
!!! CCTCACTTTTTTCAATTTAG+AGG - chr1_2:74639868-74639887 Msa0092240:CDS 30.0%
AGCATATGAAAATTATCGAG+TGG - chr1_2:74640167-74640186 Msa0092240:five_prime_UTR 30.0%
CTGAACATATTAAGAACTCT+AGG - chr1_2:74640194-74640213 Msa0092240:five_prime_UTR 30.0%
GAACATATTAAGAACTCTAG+GGG - chr1_2:74640196-74640215 Msa0092240:five_prime_UTR 30.0%
AACATATTAAGAACTCTAGG+GGG - chr1_2:74640197-74640216 Msa0092240:five_prime_UTR 30.0%
!! AAGGTTATTGTATTGGTTTC+TGG + chr1_2:74640291-74640310 None:intergenic 30.0%
! AAGCTTTAGCAAAATCCAAT+TGG + chr1_2:74640726-74640745 None:intergenic 30.0%
! ATTGGATTTTGCTAAAGCTT+TGG - chr1_2:74640726-74640745 Msa0092240:intron 30.0%
!! AGTATGTTATGTTTGTTGTC+TGG + chr1_2:74640823-74640842 None:intergenic 30.0%
! AATTTTATTCGAGGGGAAAA+AGG - chr1_2:74640870-74640889 Msa0092240:five_prime_UTR 30.0%
! ATTTTATTCGAGGGGAAAAA+GGG - chr1_2:74640871-74640890 Msa0092240:five_prime_UTR 30.0%
! ATTAAGCAAGTAGATTTTGC+TGG - chr1_2:74640980-74640999 Msa0092240:intron 30.0%
TCATTTCTCATATCTTTGTG+CGG - chr1_2:74641004-74641023 Msa0092240:intron 30.0%
AAACATAGGAAAGAGATCAA+GGG + chr1_2:74641177-74641196 None:intergenic 30.0%
TAAACATAGGAAAGAGATCA+AGG + chr1_2:74641178-74641197 None:intergenic 30.0%
ATGAACAAATTTAAGGGGTT+TGG - chr1_2:74641412-74641431 Msa0092240:intron 30.0%
TGTATTTAATTCAGGCAATC+TGG - chr1_2:74641508-74641527 Msa0092240:intron 30.0%
AAGGAATCTGAAAAACCAAA+AGG - chr1_2:74641611-74641630 Msa0092240:intron 30.0%
! TATTGTGTGACTTACTGATT+TGG - chr1_2:74641763-74641782 Msa0092240:intron 30.0%
TATAAAGCTTTGCTGAATAG+AGG - chr1_2:74641817-74641836 Msa0092240:intron 30.0%
GAAGTTAATAGTTGGATACT+TGG - chr1_2:74641915-74641934 Msa0092240:intron 30.0%
TGCATCTATAAAGTTGAAAG+CGG - chr1_2:74641939-74641958 Msa0092240:intron 30.0%
TGTTAACTTTCGCTATAGTT+GGG + chr1_2:74641977-74641996 None:intergenic 30.0%
TCATGTAATAAATACAGCTC+AGG - chr1_2:74642028-74642047 Msa0092240:intron 30.0%
TCTGCTATTAAACAGATATC+TGG - chr1_2:74642153-74642172 Msa0092240:intron 30.0%
CTATTAAACAGATATCTGGA+AGG - chr1_2:74642157-74642176 Msa0092240:intron 30.0%
TAGTAGCTGTAGAACTATTT+GGG + chr1_2:74642194-74642213 None:intergenic 30.0%
CTGAACTATTTATTTGCACA+GGG + chr1_2:74642254-74642273 None:intergenic 30.0%
TCTGAACTATTTATTTGCAC+AGG + chr1_2:74642255-74642274 None:intergenic 30.0%
! ACCAAATTTCTTTGTTATCC+AGG - chr1_2:74642807-74642826 Msa0092240:intron 30.0%
ACCTGGATAACAAAGAAATT+TGG + chr1_2:74642811-74642830 None:intergenic 30.0%
! AATTTCTTTGTTATCCAGGT+AGG - chr1_2:74642811-74642830 Msa0092240:intron 30.0%
!! TTTTAACCTAGAGAAGTACA+TGG + chr1_2:74642893-74642912 None:intergenic 30.0%
TAAAACTCCATAGTTACAGA+AGG - chr1_2:74642908-74642927 Msa0092240:intron 30.0%
TATTTATGGCCTGTTTAAAG+CGG - chr1_2:74642932-74642951 Msa0092240:five_prime_UTR 30.0%
!! ACTTGTTTACTTGGTCTTGT+GGG + chr1_2:74639444-74639463 None:intergenic 35.0%
!! AACTTGTTTACTTGGTCTTG+TGG + chr1_2:74639445-74639464 None:intergenic 35.0%
!! AAGTTCTACTGACTCAACTT+TGG - chr1_2:74639466-74639485 Msa0092240:three_prime_UTR 35.0%
TTAATCACATAATTGCGGTG+CGG + chr1_2:74639718-74639737 None:intergenic 35.0%
ACCGCAATTATGTGATTAAG+TGG - chr1_2:74639719-74639738 Msa0092240:three_prime_UTR 35.0%
GCCACTTAATCACATAATTG+CGG + chr1_2:74639723-74639742 None:intergenic 35.0%
GATCTACATACAACTATGTC+TGG - chr1_2:74639815-74639834 Msa0092240:CDS 35.0%
AGTGAGGTTACCTATGTAAA+CGG + chr1_2:74639855-74639874 None:intergenic 35.0%
ATCATGTTCGAAAATGTGAG+CGG - chr1_2:74639999-74640018 Msa0092240:intron 35.0%
ATTCTTCTCTTCATGCAGTT+TGG - chr1_2:74640079-74640098 Msa0092240:intron 35.0%
TTCTTCTCTTCATGCAGTTT+GGG - chr1_2:74640080-74640099 Msa0092240:intron 35.0%
CTGGTAATTAACATCACCAA+TGG + chr1_2:74640145-74640164 None:intergenic 35.0%
CCATTATCAAGATATGTTGC+CGG - chr1_2:74640255-74640274 Msa0092240:five_prime_UTR 35.0%
TTGCTGATATTTGACAACTC+TGG + chr1_2:74640381-74640400 None:intergenic 35.0%
GAGTTGTCAAATATCAGCAA+TGG - chr1_2:74640381-74640400 Msa0092240:intron 35.0%
ATATCAGCAATGGCAGATAT+CGG - chr1_2:74640391-74640410 Msa0092240:intron 35.0%
ATATTATCGATAGCAGATGG+CGG + chr1_2:74640537-74640556 None:intergenic 35.0%
CCAATATTATCGATAGCAGA+TGG + chr1_2:74640540-74640559 None:intergenic 35.0%
CCATCTGCTATCGATAATAT+TGG - chr1_2:74640537-74640556 Msa0092240:intron 35.0%
AATATTGGAAACCACCACAT+AGG - chr1_2:74640552-74640571 Msa0092240:intron 35.0%
ATGTTTCTTGTTCTCCTATG+TGG + chr1_2:74640569-74640588 None:intergenic 35.0%
! CTTTTATTAGAGGCGAAAGA+GGG - chr1_2:74640624-74640643 Msa0092240:intron 35.0%
TTGTCTGGAGATAATTTGAC+AGG + chr1_2:74640808-74640827 None:intergenic 35.0%
!! GGAAAAAGGGAAAGTACTAT+AGG - chr1_2:74640884-74640903 Msa0092240:five_prime_UTR 35.0%
! ACCAGAGATGACTTTGTTTA+TGG - chr1_2:74641115-74641134 Msa0092240:intron 35.0%
TCCATAAACAAAGTCATCTC+TGG + chr1_2:74641119-74641138 None:intergenic 35.0%
AGGGAGATCTAACACATTAT+AGG + chr1_2:74641158-74641177 None:intergenic 35.0%
CAATTCTTCATAGGGATAGT+TGG - chr1_2:74641370-74641389 Msa0092240:intron 35.0%
! TAGTATGGTGCATCTACTTT+TGG - chr1_2:74641468-74641487 Msa0092240:intron 35.0%
!! AGTATGGTGCATCTACTTTT+GGG - chr1_2:74641469-74641488 Msa0092240:intron 35.0%
!!! TCTACTTTTGGGTTCTGATT+TGG - chr1_2:74641480-74641499 Msa0092240:intron 35.0%
CTTGCACATTCCTAAAGATA+AGG - chr1_2:74641592-74641611 Msa0092240:intron 35.0%
CTCAGTTTCATATTCTGCAA+AGG + chr1_2:74641642-74641661 None:intergenic 35.0%
CTTTGCAGAATATGAAACTG+AGG - chr1_2:74641640-74641659 Msa0092240:intron 35.0%
TTCGTTTGTACAGTGTTACA+AGG + chr1_2:74641700-74641719 None:intergenic 35.0%
CTGTTAACTTTCGCTATAGT+TGG + chr1_2:74641978-74641997 None:intergenic 35.0%
TTACATGATTGGAAGTTCAC+AGG + chr1_2:74642016-74642035 None:intergenic 35.0%
AGTAGCTGTAGAACTATTTG+GGG + chr1_2:74642193-74642212 None:intergenic 35.0%
GTAGTAGCTGTAGAACTATT+TGG + chr1_2:74642195-74642214 None:intergenic 35.0%
CTATTTATTTGCACAGGGTA+AGG + chr1_2:74642249-74642268 None:intergenic 35.0%
! AAAATTTTCAGCATTCCGCT+AGG - chr1_2:74642274-74642293 Msa0092240:intron 35.0%
CGTGCGTTAAATTATTCTCA+AGG - chr1_2:74642324-74642343 Msa0092240:intron 35.0%
! ACTATAGTTGGAGAGCTTTT+GGG - chr1_2:74642497-74642516 Msa0092240:intron 35.0%
TCCATACACATTAGATTGGT+CGG + chr1_2:74642537-74642556 None:intergenic 35.0%
GAGATCCATACACATTAGAT+TGG + chr1_2:74642541-74642560 None:intergenic 35.0%
AATGTGTATGGATCTCACTT+TGG - chr1_2:74642545-74642564 Msa0092240:intron 35.0%
TTGGAAGTGAAAGTAGAATG+AGG - chr1_2:74642727-74642746 Msa0092240:intron 35.0%
GCTAGATATTCCATATCTAC+TGG - chr1_2:74642752-74642771 Msa0092240:intron 35.0%
TTGGTACACTACAACAACAA+TGG + chr1_2:74642792-74642811 None:intergenic 35.0%
CAAGGAAGGAAATGATAAAC+AGG + chr1_2:74642851-74642870 None:intergenic 35.0%
!! TTCCTTCCTTGTTATTTGTG+AGG - chr1_2:74642860-74642879 Msa0092240:intron 35.0%
TTCCTCACAAATAACAAGGA+AGG + chr1_2:74642865-74642884 None:intergenic 35.0%
GGTATTCCTCACAAATAACA+AGG + chr1_2:74642869-74642888 None:intergenic 35.0%
CACTTAATGACTTGACTGAT+TGG - chr1_2:74642969-74642988 Msa0092240:five_prime_UTR 35.0%
AACCTGGAATTTCACGATTC+TGG + chr1_2:74639546-74639565 None:intergenic 40.0%
CTAATTCAATGGTGCGTTGA+TGG + chr1_2:74639676-74639695 None:intergenic 40.0%
CATCAACGCACCATTGAATT+AGG - chr1_2:74639674-74639693 Msa0092240:three_prime_UTR 40.0%
! TCAAGTTGCACCGTTTACAT+AGG - chr1_2:74639842-74639861 Msa0092240:CDS 40.0%
GTATGGATTCTAGTCACCAT+TGG - chr1_2:74640126-74640145 Msa0092240:intron 40.0%
CCGGCAACATATCTTGATAA+TGG + chr1_2:74640258-74640277 None:intergenic 40.0%
ATGGGGCAAGGTTATTGTAT+TGG + chr1_2:74640298-74640317 None:intergenic 40.0%
GTACCAGCAAGTATGTTATG+GGG + chr1_2:74640315-74640334 None:intergenic 40.0%
GGTACCAGCAAGTATGTTAT+GGG + chr1_2:74640316-74640335 None:intergenic 40.0%
TGGTACCAGCAAGTATGTTA+TGG + chr1_2:74640317-74640336 None:intergenic 40.0%
CTGATATTTGACAACTCTGG+TGG + chr1_2:74640378-74640397 None:intergenic 40.0%
CTGATATGGCCAATATGTGA+AGG - chr1_2:74640433-74640452 Msa0092240:intron 40.0%
TATGGCCAATATGTGAAGGA+TGG - chr1_2:74640437-74640456 Msa0092240:intron 40.0%
TTTCTTGTTCTCCTATGTGG+TGG + chr1_2:74640566-74640585 None:intergenic 40.0%
GAACAAGAAACATAGCACAC+TGG - chr1_2:74640576-74640595 Msa0092240:intron 40.0%
CCTCTTTCGCCTCTAATAAA+AGG + chr1_2:74640626-74640645 None:intergenic 40.0%
! CCTTTTATTAGAGGCGAAAG+AGG - chr1_2:74640623-74640642 Msa0092240:intron 40.0%
!! AGGGAAAGTACTATAGGTGT+AGG - chr1_2:74640890-74640909 Msa0092240:five_prime_UTR 40.0%
! TCTGAGATTTCCTACACTGT+CGG - chr1_2:74641268-74641287 Msa0092240:intron 40.0%
ATAAAATCCACCGACAGTGT+AGG + chr1_2:74641281-74641300 None:intergenic 40.0%
TCTTCATAGGGATAGTTGGT+TGG - chr1_2:74641374-74641393 Msa0092240:intron 40.0%
! TAAGGGGTTTGGAATGTACA+CGG - chr1_2:74641423-74641442 Msa0092240:intron 40.0%
ATTAATTCAAGCTGGACGAG+TGG - chr1_2:74641565-74641584 Msa0092240:intron 40.0%
! TCTGCAAAGGCAAAACCTTT+TGG + chr1_2:74641629-74641648 None:intergenic 40.0%
! TATGCTGTCAGACTTTTCTC+AGG - chr1_2:74641674-74641693 Msa0092240:intron 40.0%
TCAGTAAGTCACACAATACG+TGG + chr1_2:74641761-74641780 None:intergenic 40.0%
TTTGCTGAATAGAGGTTCAC+AGG - chr1_2:74641825-74641844 Msa0092240:intron 40.0%
TCGTTGAGAAGAATTTGACG+AGG + chr1_2:74642220-74642239 None:intergenic 40.0%
CAAATTCTTCTCAACGACCA+AGG - chr1_2:74642223-74642242 Msa0092240:intron 40.0%
! GTCAGATATTGATTGCCTAG+CGG + chr1_2:74642292-74642311 None:intergenic 40.0%
TTAACGCACGATCTGAGAAT+CGG + chr1_2:74642314-74642333 None:intergenic 40.0%
AATGCGTATGGATCTCACTT+TGG - chr1_2:74642460-74642479 Msa0092240:intron 40.0%
GCAACCATTTGGACTATAGT+TGG - chr1_2:74642485-74642504 Msa0092240:intron 40.0%
CTCTCCAACTATAGTCCAAA+TGG + chr1_2:74642492-74642511 None:intergenic 40.0%
! GACTATAGTTGGAGAGCTTT+TGG - chr1_2:74642496-74642515 Msa0092240:intron 40.0%
! CTATAGTTGGAGAGCTTTTG+GGG - chr1_2:74642498-74642517 Msa0092240:intron 40.0%
ACCGACCAATCTAATGTGTA+TGG - chr1_2:74642533-74642552 Msa0092240:intron 40.0%
GCAACCATTTGGACTATAGT+CGG - chr1_2:74642485-74642504 Msa0092240:intron 40.0%
AGTAAGGTGGGTAAGAGATT+GGG + chr1_2:74642661-74642680 None:intergenic 40.0%
TAGTAAGGTGGGTAAGAGAT+TGG + chr1_2:74642662-74642681 None:intergenic 40.0%
! GCTTAAAGCACCAGTAGATA+TGG + chr1_2:74642765-74642784 None:intergenic 40.0%
TACATAAGCATCAACCTACC+TGG + chr1_2:74642828-74642847 None:intergenic 40.0%
ATACCTCCATGTACTTCTCT+AGG - chr1_2:74642884-74642903 Msa0092240:intron 40.0%
! TAACCTAGAGAAGTACATGG+AGG + chr1_2:74642890-74642909 None:intergenic 40.0%
! CAAAAACGACCGCTTTAAAC+AGG + chr1_2:74642944-74642963 None:intergenic 40.0%
!! CTGTTTAAAGCGGTCGTTTT+TGG - chr1_2:74642942-74642961 Msa0092240:five_prime_UTR 40.0%
! GGCGAGCTTTTGCTTTCTTA+CGG + chr1_2:74639492-74639511 None:intergenic 45.0%
AATTTCACGATTCTGGCGGT+CGG + chr1_2:74639539-74639558 None:intergenic 45.0%
CTGGAATTTCACGATTCTGG+CGG + chr1_2:74639543-74639562 None:intergenic 45.0%
! AATTTTCTTTCCCCCTCTGC+TGG - chr1_2:74639589-74639608 Msa0092240:three_prime_UTR 45.0%
TCTGCTGCGACCTAATTCAA+TGG + chr1_2:74639687-74639706 None:intergenic 45.0%
TTCTCTTCATGCAGTTTGGG+AGG - chr1_2:74640083-74640102 Msa0092240:intron 45.0%
TCTCTTCATGCAGTTTGGGA+GGG - chr1_2:74640084-74640103 Msa0092240:intron 45.0%
AGCAAGTATGTTATGGGGCA+AGG + chr1_2:74640310-74640329 None:intergenic 45.0%
TTGCCCCATAACATACTTGC+TGG - chr1_2:74640309-74640328 Msa0092240:intron 45.0%
AAGCCAAAAACCCGACATAC+CGG + chr1_2:74640503-74640522 None:intergenic 45.0%
!!! GGGTTTTTGGCTTTCCGTAA+TGG - chr1_2:74640510-74640529 Msa0092240:intron 45.0%
! CGAAAGAGGGTAAGTACTGT+AGG - chr1_2:74640637-74640656 Msa0092240:intron 45.0%
ACTGACACACAGCTCAAAGA+TGG + chr1_2:74640754-74640773 None:intergenic 45.0%
ATCTTTGAGCTGTGTGTCAG+TGG - chr1_2:74640753-74640772 Msa0092240:five_prime_UTR 45.0%
ATCTTTGTGCGGTGTGTTCA+TGG - chr1_2:74641015-74641034 Msa0092240:intron 45.0%
GATGCACCATACTACGTACA+CGG + chr1_2:74641462-74641481 None:intergenic 45.0%
ATTCAGGCAATCTGGATGAG+AGG - chr1_2:74641516-74641535 Msa0092240:intron 45.0%
TTCAGGCAATCTGGATGAGA+GGG - chr1_2:74641517-74641536 Msa0092240:intron 45.0%
CAAACGAACCCTGAAGTTTG+CGG - chr1_2:74641712-74641731 Msa0092240:intron 45.0%
! TCACTTTGGTGGCAACCATT+TGG - chr1_2:74642474-74642493 Msa0092240:intron 45.0%
! CATTAGATTGGTCGGTTGCA+CGG + chr1_2:74642529-74642548 None:intergenic 45.0%
GTGTATGGATCTCACTTTGG+TGG - chr1_2:74642548-74642567 Msa0092240:intron 45.0%
! TCACTTTGGTGGCAACCATT+TGG - chr1_2:74642474-74642493 Msa0092240:intron 45.0%
ACCATTTGGACTATAGTCGG+AGG - chr1_2:74642573-74642592 Msa0092240:intron 45.0%
CCATTTGGACTATAGTCGGA+GGG - chr1_2:74642574-74642593 Msa0092240:intron 45.0%
! ACTATAGTCGGAGGGCTTTT+GGG - chr1_2:74642582-74642601 Msa0092240:intron 45.0%
AGATTGGGCTAACTGCATCA+GGG + chr1_2:74642646-74642665 None:intergenic 45.0%
! CAACAGAGCTACAACTTGCT+CGG + chr1_2:74642997-74643016 None:intergenic 45.0%
AAAAGCTCGCCGAAAAACGC+CGG - chr1_2:74639501-74639520 Msa0092240:three_prime_UTR 50.0%
! CAAACTTCGCCGGCGTTTTT+CGG + chr1_2:74639513-74639532 None:intergenic 50.0%
CGCCAGAATCGTGAAATTCC+AGG - chr1_2:74639541-74639560 Msa0092240:three_prime_UTR 50.0%
CGAAACAGGGAGATAGTCAG+AGG + chr1_2:74639790-74639809 None:intergenic 50.0%
CTCTTCATGCAGTTTGGGAG+GGG - chr1_2:74640085-74640104 Msa0092240:intron 50.0%
!! CTCTAGGGGGTTAGTTGTCA+TGG - chr1_2:74640210-74640229 Msa0092240:five_prime_UTR 50.0%
! GGTTTCTGGAGAATCTCTGC+CGG + chr1_2:74640277-74640296 None:intergenic 50.0%
TCAGCAATGGCAGATATCGG+TGG - chr1_2:74640394-74640413 Msa0092240:intron 50.0%
CGCTGCCATCCTTCACATAT+TGG + chr1_2:74640445-74640464 None:intergenic 50.0%
GCAGTCGCCATATCCAAGTA+TGG + chr1_2:74640468-74640487 None:intergenic 50.0%
GATATGGCGACTGCCACATA+TGG - chr1_2:74640474-74640493 Msa0092240:intron 50.0%
!! TAGCAGATGGCGGACCATTA+CGG + chr1_2:74640527-74640546 None:intergenic 50.0%
GAGATTTCCTACACTGTCGG+TGG - chr1_2:74641271-74641290 Msa0092240:intron 50.0%
ACACCTCCGTGTACGTAGTA+TGG - chr1_2:74641453-74641472 Msa0092240:intron 50.0%
AGAACGCACCGCAAACTTCA+GGG + chr1_2:74641723-74641742 None:intergenic 50.0%
AAGAACGCACCGCAAACTTC+AGG + chr1_2:74641724-74641743 None:intergenic 50.0%
ACCTATGCAGCCCAGGTATA+CGG + chr1_2:74642057-74642076 None:intergenic 50.0%
CAATCAAACCTATGCAGCCC+AGG + chr1_2:74642064-74642083 None:intergenic 50.0%
ATTTGCACAGGGTAAGGCCT+TGG + chr1_2:74642243-74642262 None:intergenic 50.0%
GCGTATGGATCTCACTTTGG+TGG - chr1_2:74642463-74642482 Msa0092240:intron 50.0%
!! TAGTTGGAGAGCTTTTGGGG+AGG - chr1_2:74642501-74642520 Msa0092240:intron 50.0%
CCCTCCGACTATAGTCCAAA+TGG + chr1_2:74642577-74642596 None:intergenic 50.0%
! GACTATAGTCGGAGGGCTTT+TGG - chr1_2:74642581-74642600 Msa0092240:intron 50.0%
! CTATAGTCGGAGGGCTTTTG+GGG - chr1_2:74642583-74642602 Msa0092240:intron 50.0%
GAGATTGGGCTAACTGCATC+AGG + chr1_2:74642647-74642666 None:intergenic 50.0%
CGCGGTGGAATAGCATGCTA+AGG + chr1_2:74639627-74639646 None:intergenic 55.0%
TGCTATTCCACCGCGACTTG+CGG - chr1_2:74639632-74639651 Msa0092240:three_prime_UTR 55.0%
!! TGCGTTGATGGTCGCTATGG+TGG + chr1_2:74639664-74639683 None:intergenic 55.0%
!! TGGTGCGTTGATGGTCGCTA+TGG + chr1_2:74639667-74639686 None:intergenic 55.0%
AAGGATGGCAGCGCCATACT+TGG - chr1_2:74640452-74640471 Msa0092240:intron 55.0%
GGCAGCGCCATACTTGGATA+TGG - chr1_2:74640458-74640477 Msa0092240:intron 55.0%
ATGGCGACTGCCACATATGG+CGG - chr1_2:74640477-74640496 Msa0092240:intron 55.0%
ACATATGGCGGCCGGTATGT+CGG - chr1_2:74640489-74640508 Msa0092240:intron 55.0%
GCACCATACTACGTACACGG+AGG + chr1_2:74641459-74641478 None:intergenic 55.0%
TGGATGAGAGGGTGACAGAC+AGG - chr1_2:74641528-74641547 Msa0092240:intron 55.0%
AGGTTCACAGGCTGAGCCAT+TGG - chr1_2:74641837-74641856 Msa0092240:intron 55.0%
AGCTCAGGACACCGTATACC+TGG - chr1_2:74642043-74642062 Msa0092240:intron 55.0%
GCTCAGGACACCGTATACCT+GGG - chr1_2:74642044-74642063 Msa0092240:intron 55.0%
ACCGTATACCTGGGCTGCAT+AGG - chr1_2:74642053-74642072 Msa0092240:intron 55.0%
TGGATAACGCTAGCCGTTCC+AGG - chr1_2:74642612-74642631 Msa0092240:intron 55.0%
CGGTCGGTGACAAACTTCGC+CGG + chr1_2:74639523-74639542 None:intergenic 60.0%
TCTTTCCCCCTCTGCTGGTG+CGG - chr1_2:74639594-74639613 Msa0092240:three_prime_UTR 60.0%
GACATACCGGCCGCCATATG+TGG + chr1_2:74640490-74640509 None:intergenic 60.0%
CATATGGCGGCCGGTATGTC+GGG - chr1_2:74640490-74640509 Msa0092240:intron 60.0%
! CGGCCGGTATGTCGGGTTTT+TGG - chr1_2:74640497-74640516 Msa0092240:intron 60.0%
!! TAGTCGGAGGGCTTTTGGGG+AGG - chr1_2:74642586-74642605 Msa0092240:intron 60.0%
!! GAGGGCTTTTGGGGAGGAAC+TGG - chr1_2:74642592-74642611 Msa0092240:intron 60.0%
CTAACTGCATCAGGGCGACG+TGG + chr1_2:74642638-74642657 None:intergenic 60.0%
GTGGTTGCGACCGCAAGTCG+CGG + chr1_2:74639645-74639664 None:intergenic 65.0%
CGACTGCCACATATGGCGGC+CGG - chr1_2:74640481-74640500 Msa0092240:intron 65.0%
AGCTGCCGCACCAGCAGAGG+GGG + chr1_2:74639602-74639621 None:intergenic 70.0%
GAGCTGCCGCACCAGCAGAG+GGG + chr1_2:74639603-74639622 None:intergenic 70.0%
CGAGCTGCCGCACCAGCAGA+GGG + chr1_2:74639604-74639623 None:intergenic 70.0%
GTTGCGACCGCAAGTCGCGG+TGG + chr1_2:74639642-74639661 None:intergenic 70.0%
CAGGGCGACGTGGCCTGGAA+CGG + chr1_2:74642628-74642647 None:intergenic 70.0%
TGCATCAGGGCGACGTGGCC+TGG + chr1_2:74642633-74642652 None:intergenic 70.0%
GCGAGCTGCCGCACCAGCAG+AGG + chr1_2:74639605-74639624 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 74639438 74643040 74639438 ID=Msa0092240;Name=Msa0092240
chr1_2 mRNA 74639438 74643040 74639438 ID=Msa0092240-mRNA-1;Parent=Msa0092240;Name=Msa0092240-mRNA-1;_AED=0.07;_eAED=0.07;_QI=572|0.66|0.75|1|1|1|4|321|57
chr1_2 exon 74639438 74639974 74639438 ID=Msa0092240-mRNA-1:exon:26306;Parent=Msa0092240-mRNA-1
chr1_2 exon 74640133 74640325 74640133 ID=Msa0092240-mRNA-1:exon:26305;Parent=Msa0092240-mRNA-1
chr1_2 exon 74640745 74640956 74640745 ID=Msa0092240-mRNA-1:exon:26304;Parent=Msa0092240-mRNA-1
chr1_2 exon 74642916 74643040 74642916 ID=Msa0092240-mRNA-1:exon:26303;Parent=Msa0092240-mRNA-1
chr1_2 five_prime_UTR 74642916 74643040 74642916 ID=Msa0092240-mRNA-1:five_prime_utr;Parent=Msa0092240-mRNA-1
chr1_2 five_prime_UTR 74640745 74640956 74640745 ID=Msa0092240-mRNA-1:five_prime_utr;Parent=Msa0092240-mRNA-1
chr1_2 five_prime_UTR 74640133 74640325 74640133 ID=Msa0092240-mRNA-1:five_prime_utr;Parent=Msa0092240-mRNA-1
chr1_2 five_prime_UTR 74639933 74639974 74639933 ID=Msa0092240-mRNA-1:five_prime_utr;Parent=Msa0092240-mRNA-1
chr1_2 CDS 74639759 74639932 74639759 ID=Msa0092240-mRNA-1:cds;Parent=Msa0092240-mRNA-1
chr1_2 three_prime_UTR 74639438 74639758 74639438 ID=Msa0092240-mRNA-1:three_prime_utr;Parent=Msa0092240-mRNA-1
Gene Sequence

>Msa0092240

ATGTGTATGGATCTCACTTTGGTGGCAACCATTTGGACTATAGTCGGAGGGCTTTTGGGGAGGAACTGGATAACGCTAGCCGTTCCAGGCCACGTCGCCCTGATGCAGTTAGCCCAATCTCTTACCCACCTTACTAATATTTTCAATAGATCTGAGAGATTAAGCTTCCATTAA
Protein sequence

>Msa0092240

MCMDLTLVATIWTIVGGLLGRNWITLAVPGHVALMQLAQSLTHLTNIFNRSERLSFH*